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Optical and MRI Multimodal Tracing of Stem Cells In Vivo Mol. Imaging (IF 2.8) Pub Date : 2023-12-19 Jia Yang, Min Yan, Zhong Wang, Cong Zhang, Miao Guan, Zhenglong Sun
Stem cell therapy has shown great clinical potential in oncology, injury, inflammation, and cardiovascular disease. However, due to the technical limitations of the in vivo visualization of transplanted stem cells, the therapeutic mechanisms and biosafety of stem cells in vivo are poorly defined, which limits the speed of clinical translation. The commonly used methods for the in vivo tracing of stem
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Targeted Imaging of Endometriosis and Image-Guided Resection of Lesions Using Gonadotropin-Releasing Hormone Analogue-Modified Indocyanine Green Mol. Imaging (IF 2.8) Pub Date : 2023-12-4 Jing Peng, Qiyu Liu, Tao Pu, Mingxing Zhang, Meng Zhang, Ming Du, Guiling Li, Xiaoyan Zhang, Congjian Xu
Objective. In this study, we utilized gonadotropin-releasing hormone analogue-modified indocyanine green (GnRHa-ICG) to improve the accuracy of intraoperative recognition and resection of endometriotic lesions. Methods. Gonadotropin-releasing hormone receptor (GnRHR) expression was detected in endometriosis tissues and cell lines via immunohistochemistry and western blotting. The in vitro binding capacities
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First-in-Human PET Imaging of [18F]SDM-4MP3: A Cautionary Tale Mol. Imaging (IF 2.8) Pub Date : 2023-9-8 Kimberly L. Desmond, Anton Lindberg, Armando Garcia, Junchao Tong, Michael B. Harkness, Elena Dobrota, Kelly Smart, Carme Uribe, Jeffrey H. Meyer, Sylvain Houle, Antonio P. Strafella, Songye Li, Yiyun Huang, Neil Vasdev
[18F]SynVesT-1 is a PET radiopharmaceutical that binds to the synaptic vesicle protein 2A (SV2A) and serves as a biomarker of synaptic density with widespread clinical research applications in psychiatry and neurodegeneration. The initial goal of this study was to concurrently conduct PET imaging studies with [18F]SynVesT-1 at our laboratories. However, the data in the first two human PET studies had
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PET and SPECT Imaging of ALS: An Educational Review Mol. Imaging (IF 2.8) Pub Date : 2023-8-19 Ayaan M. Jamali, Manasa Kethamreddy, Brian J. Burkett, John D. Port, Mukesh K. Pandey
Amyotrophic lateral sclerosis (ALS) is a disease leading to progressive motor degeneration and ultimately death. It is a complex disease that can take a significantly long time to be diagnosed, as other similar pathological conditions must be ruled out for a definite diagnosis of ALS. Noninvasive imaging of ALS has shed light on disease pathology and altered biochemistry in the ALS brain. Other than
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Preliminary Assessment of Reference Region Quantification and Reduced Scanning Times for [18F]SynVesT-1 PET in Parkinson’s Disease Mol. Imaging (IF 2.8) Pub Date : 2023-8-11 Kelly Smart, Carme Uribe, Kimberly L. Desmond, Sarah L. Martin, Neil Vasdev, Antonio P. Strafella
Synaptic density in the central nervous system can be measured in vivo using PET with [18F]SynVesT-1. While [18F]SynVesT-1 has been proven to be a powerful radiopharmaceutical for PET imaging of neurodegenerative disorders such as Parkinson’s disease (PD), its currently validated acquisition and quantification protocols are invasive and technically challenging in these populations due to the arterial
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Magnetic Particle Imaging in Vascular Imaging, Immunotherapy, Cell Tracking, and Noninvasive Diagnosis Mol. Imaging (IF 2.8) Pub Date : 2023-3-15 Prashant Chandrasekharan, Renesmee Kuo, K. L. Barry Fung, Chinmoy Saayujya, Jacob Bryan, Mariam Yousuf, Benjamin Fellows, Caylin Colson, Quincy Huynh, Owen Doyle, Allison Hartley, Khadija Yousuf, Patrick Goodwill, Steven Conolly
Magnetic particle imaging (MPI) is a new tracer-based imaging modality that is useful in diagnosing various pathophysiology related to the vascular system and for sensitive tracking of cytotherapies. MPI uses nonradioactive and easily assimilated nanometer-sized iron oxide particles as tracers. MPI images the nonlinear Langevin behavior of the iron oxide particles and has allowed for the sensitive
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Development and Evaluation of a Novel Radiotracer 125I-rIL-27 to Monitor Allotransplant Rejection by Specifically Targeting IL-27Rα Mol. Imaging (IF 2.8) Pub Date : 2023-2-23 Shanshan Zhao, Qian Liu, Feng Gao, Guihua Hou
Noninvasive monitoring of allograft rejection is beneficial for the prognosis of patients with organ transplantation. Recently, IL-27/IL-27Rα was proved in close relation with inflammatory diseases, and 125I-anti-IL-27Rα mAb our group developed demonstrated high accumulation in the rejection of the allograft. However, antibody imaging has limitations in the imaging background due to its large molecular
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13-cis-Retinoic Acid Affects Brain Perfusion and Function: In Vivo Study Mol. Imaging (IF 2.8) Pub Date : 2023-2-13 Fatma Al-Saeedi, Peramaiyan Rajendran
Purpose. Study the effects of 13-cis-retinoic acid (13-RA), a synthetic analogue of a vitamin A used for the treatment of severe acne, on the blood flow in the rat brain using technetium-99m hexamethyl propylene amine oxime (99mTc-HMPAO) imaging. Methods. A total of 30 adult male Wistar rats were divided into the control (C), low-dose (L), and high-dose (H) groups. The L and H rats were exposed subcutaneously
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Immuno-PET Imaging of Siglec-15 Using the Zirconium-89-Labeled Therapeutic Antibody, NC318 Mol. Imaging (IF 2.8) Pub Date : 2023-2-9 Elaine M. Jagoda, Falguni Basuli, Colleen Olkowski, Ido Weiss, Tim E. Phelps, Karen Wong, Anita Thien Ton, Kelly C. Lane, Steve Adler, Donna Butcher, Elijah F. Edmondson, Sol Langermann, Peter L. Choyke
Objective. Sialic acid-binding immunoglobulin-like lectin 15 (Siglec-15) is overexpressed in various cancers which has led to the development of therapeutic anti-Siglec-15 monoconal antibodies (mAbs). In these preclinical studies, the therapeutic mAb, NC318 (antihuman/murine Siglec-15 mAb), was labeled with zirconium-89 and evaluated in human Siglec-15 expressing cancer cells and mouse xenografts for
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Compressed Sensing Photoacoustic Imaging Reconstruction Using Elastic Net Approach Mol. Imaging (IF 2.8) Pub Date : 2022-12-20 Xueyan Liu, Shuo Dai, Mengyu Wang, Yining Zhang
Photoacoustic imaging involves reconstructing an estimation of the absorbed energy density distribution from measured ultrasound data. The reconstruction task based on incomplete and noisy experimental data is usually an ill-posed problem that requires regularization to obtain meaningful solutions. The purpose of the work is to propose an elastic network (EN) model to improve the quality of reconstructed
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Evaluation of Intraperitoneal [18F]-FDOPA Administration for Micro-PET Imaging in Mice and Assessment of the Effect of Subchronic Ketamine Dosing on Dopamine Synthesis Capacity Mol. Imaging (IF 2.8) Pub Date : 2022-10-28 Els F. Halff, Sridhar Natesan, David R. Bonsall, Mattia Veronese, Anna Garcia-Hidalgo, Michelle Kokkinou, Sac-Pham Tang, Laura J. Riggall, Roger N. Gunn, Elaine E. Irvine, Dominic J. Withers, Lisa A. Wells, Oliver D. Howes
Positron emission tomography (PET) using the radiotracer [18F]-FDOPA provides a tool for studying brain dopamine synthesis capacity in animals and humans. We have previously standardised a micro-PET methodology in mice by intravenously administering [18F]-FDOPA via jugular vein cannulation and assessment of striatal dopamine synthesis capacity, indexed as the influx rate constant of [18F]-FDOPA, using
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Biodistribution of a Mitochondrial Metabolic Tracer, [18F]F-AraG, in Healthy Volunteers Mol. Imaging (IF 2.8) Pub Date : 2022-08-08 Jelena Levi, Heying Duan, Shahriar Yaghoubi, Juliet Packiasamy, Lyna Huynh, Tina Lam, Faiq Shaikh, Deepak Behera, Hong Song, Joseph Blecha, Salma Jivan, Youngho Seo, Henry F. VanBrocklin
Purpose. [18F]F-AraG is a radiolabeled nucleoside analog that shows relative specificity for activated T cells. The aim of this study was to investigate the biodistribution of [18F]F-AraG in healthy volunteers and assess the preliminary safety and radiation dosimetry. Methods. Six healthy subjects (three female and three male) between the ages of 24 and 60 participated in the study. Each subject received
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[18F]-(2S,4R)4-Fluoroglutamine PET Imaging of Glutamine Metabolism in Murine Models of Hepatocellular Carcinoma (HCC) Mol. Imaging (IF 2.8) Pub Date : 2022-07-25 Youngho Seo, Miranda C. Craig, Stephanie T. Murphy, Jinjin Feng, Xin Chen, Mariia Yuneva
Purpose. Quantitative in vivo [18F]-(2S,4R)4-fluoroglutamine ([18F]4-FGln or more simply [18F]FGln) metabolic kinetic parameters are compared with activity levels of glutamine metabolism in different types of hepatocellular carcinoma (HCC). Methods. For this study, we used two transgenic mouse models of HCC induced by protooncogenes, MYC, and MET. Biochemical data have shown that tumors induced by
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Performance Evaluation of a Preclinical SPECT Scanner with a Collimator Designed for Medium-Sized Animals Mol. Imaging (IF 2.8) Pub Date : 2022-07-16 Yohji Matsusaka, Rudolf A. Werner, Paula Arias-Loza, Naoko Nose, Takanori Sasaki, Xinyu Chen, Constantin Lapa, Takahiro Higuchi
Background. Equipped with two stationary detectors, a large bore collimator for medium-sized animals has been recently introduced for dedicated preclinical single-photon emission computed tomography (SPECT) imaging. We aimed to evaluate the basic performance of the system using phantoms and healthy rabbits. Methods. A general-purpose medium-sized animal (GP-MSA) collimator with 135 mm bore diameter
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Preclinical Evaluation of an Activity-Based Probe for Intraoperative Imaging of Esophageal Cancer Mol. Imaging (IF 2.8) Pub Date : 2022-07-14 Gregory T. Kennedy, Feredun S. Azari, Bilal Nadeem, Ashley Chang, Alix Segil, Elizabeth Bernstein, Charuhas Desphande, John C. Kucharczuk, Edward J. Delikatny, Sunil Singhal
Background. Early detection and complete resection are important prognostic factors for esophageal cancer (EC). Intraoperative molecular imaging (IMI) using tumor-targeted tracers is effective in many cancer types. However, there are no EC-specific IMI tracers. We sought to test a cathepsin activity-based tracer (VGT-309) for EC resection. Methods. Murine (AKR, HNM007) and human (OE19) EC cell lines
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In Vivo Distribution and Therapeutic Efficacy of Radioiodine-Labeled pH-Low Insertion Peptide Variant 3 in a Mouse Model of Breast Cancer Mol. Imaging (IF 2.8) Pub Date : 2022-07-04 Min Zhang, Yue Xi, Hong Chen, Wangxi Hai, Biao Li
Purpose. Extracellular acidity is a marker of highly aggressive breast cancer (BC). pH-low insertion peptides (pHLIPs) target the acidic tumor microenvironment. This study evaluates the distribution and therapeutic efficacy of radioiodine-labeled pHLIP variant 3 (Var3) in a mouse model of BC. Methods. The binding of fluorescein isothiocyanate (FITC)- or radioiodine-125 (125I) labeled Var3-pHLIP to
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Application of Dynamic 18F-FDG PET/CT for Distinguishing Intrapulmonary Metastases from Synchronous Multiple Primary Lung Cancer Mol. Imaging (IF 2.8) Pub Date : 2022-06-30 Weize Lv, Min Yang, Hongcheng Zhong, Xiaojin Wang, Shuai Yang, Lei Bi, Jianzhong Xian, Xiaofeng Pei, Xinghua He, Ying Wang, Zhong Lin, Qingdong Cao, Hongjun Jin, Hong Shan
It has been a big challenge to distinguish synchronous multiple primary lung cancer (sMPLC) from primary lung cancer with intrapulmonary metastases (IPM). We aimed to assess the clinical application of dynamic 18F-FDG PET/CT in patients with multiple lung cancer nodules. We enrolled patients with multiple pulmonary nodules who had undergone dynamic 18F-FDG PET/CT and divided them into sMPLC and IPM
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Noninvasive Evaluation of EGFR Expression of Digestive Tumors Using 99mTc-MAG3-Cet-F(ab)2-Based SPECT/CT Imaging Mol. Imaging (IF 2.8) Pub Date : 2022-06-24 Dai Shi, Yiqiu Zhang, Zhan Xu, Zhan Si, Yuan Cheng, Dengfeng Cheng, Guobing Liu
Purpose. This study is aimed at investigating the feasibility of cetuximab (Cet) F(ab)2 fragment- (Cet-F(ab)2-) based single photon emission tomography/computed tomography (SPECT/CT) for assessing the epidermal growth factor receptor (EGFR) expression in digestive tumor mouse models. Methods. Cet-F(ab)2 was synthesized using immunoglobulin G-degrading enzyme of Streptococcus pyogenes (IdeS) protease
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In Vivo Functional Assessment of Sodium-Glucose Cotransporters (SGLTs) Using [18F]Me4FDG PET in Rats Mol. Imaging (IF 2.8) Pub Date : 2022-06-21 Yohji Matsusaka, Xinyu Chen, Paula Arias-Loza, Rudolf A. Werner, Naoko Nose, Takanori Sasaki, Steven P. Rowe, Martin G. Pomper, Constantin Lapa, Takahiro Higuchi
Background. Mediating glucose absorption in the small intestine and renal clearance, sodium glucose cotransporters (SGLTs) have emerged as an attractive therapeutic target in diabetic patients. A substantial fraction of patients, however, only achieve inadequate glycemic control. Thus, we aimed to assess the potential of the SGLT-targeting PET radiotracer alpha-methyl-4-deoxy-4-[18F]fluoro-D-glucopyranoside
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Neuroinflammation in Low-Level PM2.5-Exposed Rats Illustrated by PET via an Improved Automated Produced [18F]FEPPA: A Feasibility Study Mol. Imaging (IF 2.8) Pub Date : 2022-06-07 Mei-Fang Cheng, Tsun-Jen Cheng, Yue Leon Guo, Ching-Hung Chiu, Hung-Ming Wu, Ruoh-Fang Yen, Ya-Yao Huang, Wen-Sheng Huang, Chyng-Yann Shiue
Background. [18F]FEPPA is a potent TSPO imaging agent that has been found to be a potential tracer for imaging neuroinflammation. In order to fulfill the demand of this tracer for preclinical and clinical studies, we have developed a one-pot automated synthesis with simplified HPLC purification of this tracer, which was then used for PET imaging of neuroinflammation in fine particulate matter- (PM2
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Pattern of F-18 FDG Uptake in Colon Cancer after Bacterial Cancer Therapy Using Engineered Salmonella Typhimurium: A Preliminary In Vivo Study Mol. Imaging (IF 2.8) Pub Date : 2022-04-19 Ari Chong, Dinh-Huy Nguyen, Hyeon Sik Kim, June-Key Chung, Jung-Joon Min
Purpose. Bacterial cancer therapy (BCT) research using engineered Salmonella typhimurium has increased in recent years. 2-Deoxy-2[18F] fluoro-D-glucose positron emission tomography (FDG PET) is widely used in cancer patients to detect cancer, monitor treatment responses, and predict prognoses. The aim of this pilot study was to investigate FDG uptake patterns in a mouse tumor model after BCT. Procedures
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Prostate-Specific Membrane Antigen as Target for Neuroimaging of Central Nervous System Tumors Mol. Imaging (IF 2.8) Pub Date : 2022-04-15 Brittany M. Stopa, James Crowley, Csaba Juhász, Cara M. Rogers, Mark R. Witcher, Jackson W. Kiser
Introduction. Positron emission tomography (PET) imaging with prostate-specific membrane antigen- (PSMA-) binding tracers has been found incidentally to demonstrate uptake in CNS tumors. Following the encouraging findings of several such case reports, there is a growing interest in the potential application of PSMA-targeted PET imaging for diagnostics, theranostics, and monitoring of CNS tumors. This
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Near-Infrared Fluorescence Imaging of EGFR-Overexpressing Tumors in the Mouse Xenograft Model Using scFv-IRDye800CW and Cetuximab-IRDye800CW Mol. Imaging (IF 2.8) Pub Date : 2022-04-14 Abolfazl Amini, Yaghoub Safdari, Fatemeh Tash Shamsabadi
EGFR (epidermal growth factor receptor) is overexpressed in a variety of human cancers (including squamous cell carcinoma of head and neck, colon cancer, and some breast cancers) and therefore is regarded as an ideal target for cancer therapy or imaging purposes. In the current study, we produced a scFv-based near-infrared probe (called cet.Hum.scFv-IRDye-800CW) and evaluated its ability in recognizing
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The Probe for Renal Organic Cation Secretion (4-Dimethylaminostyryl)-N-Methylpyridinium (ASP+)) Shows Amplified Fluorescence by Binding to Albumin and Is Accumulated In Vivo Mol. Imaging (IF 2.8) Pub Date : 2022-03-22 Jacob Schade Engbjerg, Vincenzo Costanzo, Donato Sardella, Luca Bordoni, Steen Jakobsen, Luciano D'Apolito, Jørgen Frøkiær, Francesco Trepiccione, Giovambattista Capasso, Sebastian Frische
Accumulation of uremic toxins may lead to the life-threatening condition “uremic syndrome” in patients with advanced chronic kidney disease (CKD) requiring renal replacement therapy. Clinical evaluation of proximal tubular secretion of organic cations (OC), of which some are uremic toxins, is desired, but difficult. The biomedical knowledge on OC secretion and cellular transport partly relies on studies
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The Potential Prognostic Value of Dual-Imaging PET Parameters Based on 18F-FDG and 18F-OC for Neuroendocrine Neoplasms Mol. Imaging (IF 2.8) Pub Date : 2022-03-17 Jiale Hou, Tingting Long, Yi Yang, Dengming Chen, Shuo Hu
Background. To identify parameters based on dual-imaging 18F-AlF-NOTA-octreotide (18F-OC) and 18-fluorodeoxyglucose (18F-FDG) positron emission tomography (PET) for predicting the prognosis of neuroendocrine neoplasms (NENs). Materials and Methods. Sixty-six patients (age: (SD): years) who underwent both 18F-OC and 18F-FDG PET/CT imaging were enrolled in our retrospective study. The following PET parameters
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Evaluation of [18F]tetrafluoroborate as a Potential PET Imaging Agent in a Sodium Iodide Symporter-Transfected Cell Line A549 and Endogenous NIS-Expressing Cell Lines MKN45 and K1 Mol. Imaging (IF 2.8) Pub Date : 2022-02-27 Mengda Niu, Jingjing Qin, Liang Wang, Yujia He, Chuanhuizi Tian, Yanyan Chen, Pufeng Huang, Zhiping Peng
[18F]tetrafluoroborate (TFB) has been introduced as the 18F-labeled PET imaging probe for the human sodium iodide symporter (NIS). Noninvasive NIS imaging using [18F]TFB has received much interest in recent years for evaluating various NIS-expressing tumors. Cancers are a global concern with enormous implications; therefore, improving diagnostic methods for accurate detection of cancer is extremely
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High SUVs Have More Robust Repeatability in Patients with Metastatic Prostate Cancer: Results from a Prospective Test-Retest Cohort Imaged with 18F-DCFPyL Mol. Imaging (IF 2.8) Pub Date : 2022-02-23 Rudolf A. Werner, Bilêl Habacha, Susanne Lütje, Lena Bundschuh, Takahiro Higuchi, Philipp Hartrampf, Sebastian E. Serfling, Thorsten Derlin, Constantin Lapa, Andreas K. Buck, Markus Essler, Kenneth J. Pienta, Mario A. Eisenberger, Mark C. Markowski, Laura Shinehouse, Rehab AbdAllah, Ali Salavati, Martin A. Lodge, Martin G. Pomper, Michael A. Gorin, Ralph A. Bundschuh, Steven P. Rowe
Objectives. In patients with prostate cancer (PC) receiving prostate-specific membrane antigen- (PSMA-) targeted radioligand therapy (RLT), higher baseline standardized uptake values (SUVs) are linked to improved outcome. Thus, readers deciding on RLT must have certainty on the repeatability of PSMA uptake metrics. As such, we aimed to evaluate the test-retest repeatability of lesion uptake in a large
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89Zr Immuno-PET Imaging of Tumor PD-1 Reveals That PMA Upregulates Lymphoma PD-1 through NFκB and JNK Signaling Mol. Imaging (IF 2.8) Pub Date : 2022-02-12 Kyung-Ho Jung, Jin Hee Lee, Mina Kim, Young Seok Cho, Kyung-Han Lee
Immune therapy of T-cell lymphoma requires assessment of tumor-expressed programmed cell death protein-1 (PD-1). Herein, we developed an immuno-PET technique that quantitatively images and monitors regulation of PD-1 expression on T-cell lymphomas. Methods. Anti-PD-1 IgG underwent sulfhydryl moiety-specific conjugation with maleimide-deferoxamine and 89Zr labeling. Binding assays and Western blotting
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Positron Emission Tomography (PET) with 18F-FGA for Diagnosis of Myocardial Infarction in a Coronary Artery Ligation Model Mol. Imaging (IF 2.8) Pub Date : 2022-02-09 Vibhudutta Awasthi, Hariprasad Gali, Andria F. Hedrick, Huining Da, Venkateswararao Eeda, Diwakar Jain
Location and extent of necrosis are valuable information in the management of myocardial infarction (MI). Methods. We investigated 2-deoxy-2-18F-fluoro glucaric acid (FGA), a novel infarct-avid agent, for positron emission tomography (PET) of MI. We synthesized FGA from commercially available 18F-fluoro-2-deoxy-2-D-glucose (FDG). MI was induced in mice by permanently occluding the left anterior descending
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Celecoxib-Induced Modulation of Colon Cancer CD133 Expression Occurs through AKT Inhibition and Is Monitored by 89Zr Immuno-PET Mol. Imaging (IF 2.8) Pub Date : 2022-01-07 Kyung-Ho Jung, Jin Hee Lee, Mina Kim, Eun Ji Lee, Young Seok Cho, Kyung-Han Lee
We developed an immuno-PET technique that monitors modulation of tumor CD133 expression, which is required for the success of CD133-targeted therapies. Methods. Anti-CD133 antibodies were subjected to sulfhydryl moiety-specific 89Zr conjugation. 89Zr-CD133 IgG was evaluated for specific activity and radiolabel stability. Colon cancer cells underwent binding assays and Western blotting. Biodistribution
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Granzyme B PET Imaging Stratifies Immune Checkpoint Inhibitor Response in Hepatocellular Carcinoma Mol. Imaging (IF 2.8) Pub Date : 2021-12-09 Julian L. Goggi, Boominathan Ramasamy, Yun Xuan Tan, Siddesh V. Hartimath, Jun Rong Tang, Peter Cheng, Rasha Msallam, Ann-Marie Chacko, You Yi Hwang, Edward G. Robins
Hepatocellular carcinoma (HCC) is a notoriously difficult cancer to treat. The recent development of immune checkpoint inhibitors has revolutionised HCC therapy; however, successful response is only observed in a small percentage of patients. Biomarkers typically used to predict treatment response in other tumour types are ineffective in HCC, which arises in an immune-suppressive environment. However
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The Number of Frames on ECG-Gated 18F-FDG Small Animal PET Has a Significant Impact on LV Systolic and Diastolic Functional Parameters Mol. Imaging (IF 2.8) Pub Date : 2021-12-06 Christoph Eissler, Rudolf A. Werner, Paula Arias-Loza, Naoko Nose, Xinyu Chen, Martin G. Pomper, Steven P. Rowe, Constantin Lapa, Andreas K. Buck, Takahiro Higuchi
Objectives. This study is aimed at investigating the impact of frame numbers in preclinical electrocardiogram- (ECG-) gated 18F-fluorodeoxyglucose (18F-FDG) positron emission tomography (PET) on systolic and diastolic left ventricular (LV) parameters in rats. Methods. 18F-FDG PET imaging using a dedicated small animal PET system with list mode data acquisition and continuous ECG recording was performed
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Comparison of 18F-FDG, 18F-Fluoroacetate, and 18F-FEPPA for Imaging Liver Fibrosis in a Bile Duct-Ligated Rat Model Mol. Imaging (IF 2.8) Pub Date : 2021-11-27 Chun-Yi Wu, Hsin-Hua Hsieh, Pei-An Chu, Wen-Hsiang Hong, Ting-Yu Chang, Chia-Fang Hsu, Siao-Ting Lin, Po-Hsun Su, Shin-Lei Peng
Developing sensitive diagnostic methods for a longitudinal evaluation of the status of liver fibrosis is a priority. This study is aimed at assessing the significance of longitudinal positron emission tomography (PET) imaging with 18F-labeling tracers for assessing liver fibrosis in a rat model with bile duct ligation (BDL). Twenty-one 6-week-old Sprague-Dawley male rats were used in this study. Longitudinal
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PET Study of Sphingosine-1-phosphate Receptor 1 Expression in Response to S. aureus Infection Mol. Imaging (IF 2.8) Pub Date : 2021-10-04 Hao Jiang, Jiwei Gu, Haiyang Zhao, Sumit Joshi, Joel S. Perlmutter, Robert J. Gropler, Robyn S. Klein, Tammie L. S. Benzinger, Zhude Tu
Sphingosine-1-phosphate receptor 1 (S1PR1) plays a crucial role in infectious diseases. Targeting S1PR1 provides protection against pathogens, such as influenza viruses. This study is aimed at investigating S1PR1 in response to bacterial infection by assessing S1PR1 expression in S. aureus-infected mice. A rodent local muscle bacterial infection model was developed by injecting S. aureus to the lower
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The Evaluation of Tau Deposition with [18F]PI-2620 by Using a Semiquantitative Method in Cognitively Normal Subjects and Patients with Mild Cognitive Impairment and Alzheimer’s Disease Mol. Imaging (IF 2.8) Pub Date : 2021-07-10 Attapon Jantarato, Sira Vachatimanont, Natphimol Boonkawin, Sukanya Yaset, Anchisa Kunawudhi, Chetsadaporn Promteangtrong, Jintana Assanasen, Nithi Mahanonda, Chanisa Chotipanich
Background. Some studies have reported the effectiveness of [18F]PI-2620 as an effective tau-binding radiotracer; however, few reports have applied semiquantitative analysis to the tracer. Therefore, this study’s aim was to perform a semiquantitative analysis of [18F]PI-2620 in individuals with normal cognition and patients with mild cognitive impairment (MCI) and Alzheimer’s disease (AD). Methods
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Automated Synthesis and Initial Evaluation of (4-Amino-5,8-difluoro-1H-spiro[piperidine-4,2-quinazolin]-1-yl)(4-[18F]fluorophenyl)methanone for PET/MR Imaging of Inducible Nitric Oxide Synthase Mol. Imaging (IF 2.8) Pub Date : 2021-07-08 Skye Hsin-Hsien Yeh, Wen-Sheng Huang, Chuang-Hsin Chiu, Chuan-Lin Chen, Hui-Ting Chen, Dae Yoon Chi, Zhengxing Ge, Tsung-Hsun Yu, Pao-Yeh Wang, Yu-Yeh Kuo, Chun-Tse Hung, Geng-Ying Li, Chi-Wei Chang
Background. Inducible nitric oxide synthase (iNOS) plays a crucial role in neuroinflammation, especially microglial activity, and may potentially represent a useful biomarker of neuroinflammation. In this study, we carefully defined a strategic plan to develop iNOS-targeted molecular PET imaging using (4-amino-5,8-difluoro-1H-spiro[piperidine-4,2-quinazolin]-1-yl)(4-fluorophenyl)methanone ([18F]FBAT)
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A Photoacoustic Imaging Algorithm Based on Regularized Smoothed L0 Norm Minimization Mol. Imaging (IF 2.8) Pub Date : 2021-06-01 Xueyan Liu, Limei Zhang, Yining Zhang, Lishan Qiao
The recently emerging technique of sparse reconstruction has received much attention in the field of photoacoustic imaging (PAI). Compressed sensing (CS) has large potential in efficiently reconstructing high-quality PAI images with sparse sampling signal. In this article, we propose a CS-based error-tolerant regularized smooth L0 (ReSL0) algorithm for PAI image reconstruction, which has the same computational
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Early Changes in [18F]FDG Uptake as a Readout for PI3K/Akt/mTOR Targeted Drugs in HER-2-Positive Cancer Xenografts Mol. Imaging (IF 2.8) Pub Date : 2021-05-25 Yanina Dockx, Christel Vangestel, Tim Van den Wyngaert, Manon Huizing, Sven De Bruycker, Patrick Pauwels, Steven Staelens, Sigrid Stroobants
We investigated the potential use of [18F]FDG PET as a response biomarker for PI3K pathway targeting therapies in two HER-2-overexpressing cancer models. Methods. CD-1 nude mice were inoculated with HER-2-overexpressing JIMT1 (trastuzumab-resistant) or SKOV3 (trastuzumab-sensitive) human cancer cells. Animals were treated with trastuzumab, everolimus (mTOR inhibitor), PIK90 (PI3K inhibitor), saline
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In Vivo Evaluation of the Combined Anticancer Effects of Cisplatin and SAHA in Nonsmall Cell Lung Carcinoma Using [18F]FAHA and [18F]FDG PET/CT Imaging Mol. Imaging (IF 2.8) Pub Date : 2021-04-01 Skye Hsin-Hsien Yeh, Ming Hsien Lin, I. I. Leo Garcia Flores, Uday Mukhopadhyay, Danial Young, Kazuma Ogawa, Jeong-Hwan Jeong, William Tong, Juri G. Gelovani, Nobuyoshi Fukumitsu
Combining standard drugs with low doses of histone deacetylase inhibitors (HDACIs) is a promising strategy to increase the efficacy of chemotherapy. The ability of well-tolerated doses of HDACIs that act as chemosensitizers for platinum-based chemotherapeutics has recently been proven in many types and stages of cancer in vitro and in vivo. Detection of changes in HDAC activity/expression may provide
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In Vivo Evaluation of the Combined Anticancer Effects of Cisplatin and SAHA in Nonsmall Cell Lung Carcinoma Using [18F]FAHA and [18F]FDG PET/CT Imaging. Mol. Imaging (IF 2.8) Pub Date : 2021-03-31 Skye Hsin-Hsien Yeh,Ming Hsien Lin,I I Leo Garcia Flores,Uday Mukhopadhyay,Danial Young,Kazuma Ogawa,Jeong-Hwan Jeong,William Tong,Juri G Gelovani,Nobuyoshi Fukumitsu
Combining standard drugs with low doses of histone deacetylase inhibitors (HDACIs) is a promising strategy to increase the efficacy of chemotherapy. The ability of well-tolerated doses of HDACIs that act as chemosensitizers for platinum-based chemotherapeutics has recently been proven in many types and stages of cancer in vitro and in vivo. Detection of changes in HDAC activity/expression may provide
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Use of Innovative SPECT Techniques in the Presurgical Evaluation of Patients with Nonlesional Extratemporal Drug-Resistant Epilepsy Mol. Imaging (IF 2.8) Pub Date : 2021-03-04 Ahmed Yassin, Khalid El-Salem, Abdel-Hameed Al-Mistarehi, Aiman Momani, Anas M. Zein Alaabdin, Palak Shah, James Michael Mountz, Anto I. Bagić
Up to 30% of patients with epilepsy may not respond to antiepileptic drugs. Patients with drug-resistant epilepsy (DRE) should undergo evaluation for seizure onset zone (SOZ) localization to consider surgical treatment. Cases of drug-resistant nonlesional extratemporal lobe epilepsy (ETLE) pose the biggest challenge in localizing the SOZ and require multiple noninvasive diagnostic investigations before
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An Experimental Study on [125I]I-pHLIP (Var7) for SPECT/CT Imaging of an MDA-MB-231 Triple-Negative Breast Cancer Mouse Model by Targeting the Tumor Microenvironment Mol. Imaging (IF 2.8) Pub Date : 2021-02-16 Mingming Yu, Yanqin Sun, Guangjie Yang, Zhenguang Wang
Objective. To evaluate the diagnostic efficacy of MDA-MB-231 triple-negative breast cancer with 125I-labeled pHLIP (Var7) by single-photon emission computed tomography/computed tomography (SPECT/CT) imaging. Methods. The binding fraction of [125I]I-pHLIP (Var7) and MDA-MB-231 cells was measured at pH 7.4 and pH 6.0, and tumor-bearing mice were subjected to small-animal SPECT/CT imaging studies. Results
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Comparison of HER2-Targeted Antibodies for Fluorescence-Guided Surgery in Breast Cancer Mol. Imaging (IF 2.8) Pub Date : 2021-02-02 Solmaz AghaAmiri, Jo Simien, Alastair M. Thompson, Julie Voss, Sukhen C. Ghosh, Servando Hernandez Vargas, Sarah Kim, Ali Azhdarinia, Hop S. Tran Cao
Background. Although therapeutic advances have led to enhanced survival in patients with human epidermal growth factor receptor 2 (HER2)-positive breast cancer, detection of residual disease remains challenging. Here, we examine two approved anti-HER2 monoclonal antibodies (mAbs), trastuzumab and pertuzumab, as potential candidates for the development of immunoconjugates for fluorescence-guided surgery
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Comparison of Amino Acid PET to Advanced and Emerging MRI Techniques for Neurooncology Imaging: A Systematic Review of the Recent Studies Mol. Imaging (IF 2.8) Pub Date : 2021-01-20 Brittany M. Stopa, Csaba Juhász, Sandeep Mittal
Introduction. Standard neuroimaging protocols for brain tumors have well-known limitations. The clinical use of additional modalities including amino acid PET (aaPET) and advanced MRI (aMRI) techniques (including DWI, PWI, and MRS) is emerging in response to the need for more accurate detection of brain tumors. In this systematic review of the past 2 years of the literature, we discuss the most recent
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[18F]PARPi Imaging Is Not Affected by HPV Status In Vitro. Mol. Imaging (IF 2.8) Pub Date : 2021-01-20 Navjot Guru,Paula Demétrio De Souza França,Giacomo Pirovano,Cien Huang,Snehal G Patel,Thomas Reiner
Background Human papillomavirus- (HPV-) associated oropharyngeal squamous cell carcinomas (OPSCCs) are clinically and pathologically distinct from HPV-negative tumors. Here, we explore whether HPV affects functional biomarkers, including γH2AX, RAD51, and PARP1. Moreover, the role of [18F]PARPi as a broadly applicable imaging tool for head and neck carcinomas is investigated. Methods HPV-positive and
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Advances in Quantitative Analysis of 18F-Sodium Fluoride Coronary Imaging. Mol. Imaging (IF 2.8) Pub Date : 2021-01-15 Jacek Kwiecinski,Martin Lyngby Lassen,Piotr J Slomka
18F-sodium fluoride (18F-NaF) positron emission tomography (PET) has emerged as a promising noninvasive imaging tool for the assessment of active calcification processes in coronary artery disease. 18F-NaF uptake colocalizes to high-risk and ruptured atherosclerotic plaques. Most recently, 18F-NaF coronary uptake was shown to be a robust and independent predictor of myocardial infarction in patients
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Optimization of In Vivo Studies by Combining Planar Dynamic and Tomographic Imaging: Workflow Evaluation on a Superparamagnetic Nanoparticles System. Mol. Imaging (IF 2.8) Pub Date : 2021-01-15 Maritina Rouchota,Alessio Adamiano,Michele Iafisco,Eirini Fragogeorgi,Irineos Pilatis,Gilles Doumont,Sébastien Boutry,Daniele Catalucci,Argyro Zacharioudaki,George C Kagadis
Molecular imaging holds great promise in the noninvasive monitoring of several diseases with nanoparticles (NPs) being considered an efficient imaging tool for cancer, central nervous system, and heart- or bone-related diseases and for disorders of the mononuclear phagocytic system (MPS). In the present study, we used an iron-based nanoformulation, already established as an MRI/SPECT probe, as well
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Sound Out the Deep Colors: Photoacoustic Molecular Imaging at New Depths Mol. Imaging (IF 2.8) Pub Date : 2020-12-18 Mucong Li, Nikhila Nyayapathi, Hailey I. Kilian, Jun Xia, Jonathan F. Lovell, Junjie Yao
Photoacoustic tomography (PAT) has become increasingly popular for molecular imaging due to its unique optical absorption contrast, high spatial resolution, deep imaging depth, and high imaging speed. Yet, the strong optical attenuation of biological tissues has traditionally prevented PAT from penetrating more than a few centimeters and limited its application for studying deeply seated targets. A
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High-Efficiency Production of Radiopharmaceuticals via Droplet Radiochemistry: A Review of Recent Progress Mol. Imaging (IF 2.8) Pub Date : 2020-12-09 Jia Wang, R. Michael van Dam
New platforms are enabling radiochemistry to be carried out in tiny, microliter-scale volumes, and this capability has enormous benefits for the production of radiopharmaceuticals. These droplet-based technologies can achieve comparable or better yields compared to conventional methods, but with vastly reduced reagent consumption, shorter synthesis time, higher molar activity (even for low activity
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Targeted Imaging Agent to HSP70 Induced In Vivo Mol. Imaging (IF 2.8) Pub Date : 2020-11-20 Pradip Ghosh, Brian E. O’Neill, King C. Li
Heat shock protein expression can be induced by heat shock making it possible to artificially modulate their levels noninvasively in vivo in a spatially and temporally controlled manner. Here, we report the use of the major heat shock protein 70 (HSP70) as an inducible target by using the small molecule deoxyspergualin (DSG) conjugated to the near-infrared fluorophore (Cy5.5). We demonstrate that heat
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Molecular MR-Imaging for Noninvasive Quantification of the Anti-Inflammatory Effect of Targeting Interleukin-1β in a Mouse Model of Aortic Aneurysm Mol. Imaging (IF 2.8) Pub Date : 2020-11-20 Julia Brangsch, Carolin Reimann, Jan Ole Kaufmann, Lisa Christine Adams, David Onthank, Christa Thöne-Reineke, Simon Robinson, Marco Wilke, Michael Weller, Rebecca Buchholz, Uwe Karst, Rene Botnar, Bernd Hamm, Marcus Richard Makowski
Background: Molecular-MRI is a promising imaging modality for the assessment of abdominal aortic aneurysms (AAAs). Interleukin-1β (IL-1β) represents a new therapeutic tool for AAA-treatment, since pro-inflammatory cytokines are key-mediators of inflammation. This study investigates the potential of molecular-MRI to evaluate therapeutic effects of an anti-IL-1β-therapy on AAA-formation in a mouse-model
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[11C]Methionine and [11C]PBR28 as PET Imaging Tracers to Differentiate Metastatic Tumor Recurrence or Radiation Necrosis Mol. Imaging (IF 2.8) Pub Date : 2020-11-04 Thuy T. Tran, Jean-Dominique Gallezot, Lucia B. Jilaveanu, Christopher Zito, Gabriela Turcu, Keunpoong Lim, Nabeel Nabulsi, Henry Huang, Anita Huttner, Harriet M. Kluger, Veronica L. Chiang, Richard Carson
Purpose: As stereotactic radiosurgery (SRS) and immunotherapy are increasingly used to treat brain metastases, incidence of radiation necrosis (RN) is consequently rising. Differentiating tumor regrowth (TR) from RN is vital in management but difficult to assess using MRI. We hypothesized that tumor methionine levels would be elevated given increased metabolism and high amino acid uptake, whereas RN
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Multi-Wavelength Fluorescence in Image-Guided Surgery, Clinical Feasibility and Future Perspectives Mol. Imaging (IF 2.8) Pub Date : 2020-10-30 Florian van Beurden, Danny M. van Willigen, Borivoj Vojnovic, Matthias N. van Oosterom, Oscar R. Brouwer, Henk G. van der Poel, Hisataka Kobayashi, Fijs W.B. van Leeuwen, Tessa Buckle
With the rise of fluorescence-guided surgery, it has become evident that different types of fluorescence signals can provide value in the surgical setting. Hereby a different range of targets have been pursued in a great variety of surgical indications. One of the future challenges lies in combining complementary fluorescent readouts during one and the same surgical procedure, so-called multi-wavelength
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From the Outside in: An Overview of Positron Imaging of Plant and Soil Processes Mol. Imaging (IF 2.8) Pub Date : 2020-10-29 Michael P. Schmidt, Steven D. Mamet, Richard A. Ferrieri, Derek Peak, Steven D. Siciliano
Positron-emitting nuclides have long been used as imaging agents in medical science to spatially trace processes non-invasively, allowing for real-time molecular imaging using low tracer concentrations. This ability to non-destructively visualize processes in real time also makes positron imaging uniquely suitable for probing various processes in plants and porous environmental media, such as soils
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Magnetofluorescent Nanoprobe for Multimodal and Multicolor Bioimaging Mol. Imaging (IF 2.8) Pub Date : 2020-10-28 Aditya Yadav, Chethana Rao, Navneet Chandra Verma, Pushpendra Mani Mishra, Chayan Kanti Nandi
Although, superparamagnetic iron oxide nanoparticles (SPIONs) have extensively been used as a contrasting agent for magnetic resonance imaging (MRI), the lack of intrinsic fluorescence restricted their application as a multimodal probe, especially in combination with light microscopy. In Addition, the bigger size of the particle renders them incompetent for bioimaging of small organelles. Herein, we
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Two Patient Studies of a Companion Diagnostic Immuno-Positron Emission Tomography (PET) Tracer for Measuring Human CA6 Expression in Cancer for Antibody Drug Conjugate (ADC) Therapy Mol. Imaging (IF 2.8) Pub Date : 2020-10-26 Arutselvan Natarajan, Shyam M. Srinivas, Carmen Azevedo, Lacey Greene, Anne-Laure Bauchet, Erwan Jouannot, Anne-Sophie Lacoste-Bourgeacq, Isabelle Guizon, Patrick Cohen, Anne-Laure Naneix, Ohad Ilovich, Jordan Cisneros, Krithika Rupanarayan, Frederick T. Chin, Andrei Iagaru, Frederick M. Dirbas, Amer Karam, Sanjiv S. Gambhir
An antigen binding fragment (BFab) derived from a tumor-associated mucin 1–sialoglycotope antigen (CA6) targeting antibody (huDS6) was engineered. We synthesized a companion diagnostic positron emission tomography (PET) tracer by radiolabeling BFab with [64Cu] to measure CA6 expression on cancer tissues prior to anti-human CA6 (huDS6-DM4 antibody-drug conjugate) therapy for ovarian and breast cancer
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A Decade’s Progress in the Development of Molecular Imaging Agents Targeting the Growth Hormone Secretagogue Receptor Mol. Imaging (IF 2.8) Pub Date : 2020-10-26 Marina D. Childs, Leonard G. Luyt
The growth hormone secretagogue receptor 1a (GHSR), also called the ghrelin receptor, is a G protein-coupled receptor known to play an important metabolic role in the regulation of various physiological processes, including energy expenditure, growth hormone secretion, and cell proliferation. This receptor has been implicated in numerous health issues including obesity, gastrointestinal disorders,
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Peptide Based Imaging Agents for HER2 Imaging in Oncology. Mol. Imaging (IF 2.8) Pub Date : 2020-09-22 Maxwell Ducharme,Suzanne E Lapi
Breast cancer continues to be the most lethal cancer type in women and one of the most diagnosed. Understanding Breast cancer receptor status is one of the most vital processes for determining treatment options. One type of breast cancer, human epidermal growth factor receptor 2 (HER2) positive, has approved receptor-based therapies including trastuzumab and pertuzumab that can significantly increase
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Radionuclide Imaging for Neuroscience: Current Opinion and Future Directions. Mol. Imaging (IF 2.8) Pub Date : 2020-09-09 Antony D Gee,Matthias M Herth,Michelle L James,Aruna Korde,Peter J H Scott,Neil Vasdev
This meeting report summarizes a Consultants Meeting that was held at International Atomic Energy Agency headquarters in Vienna to provide an update on radionuclide imaging for neuroscience applications.
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Research Progress of Radiolabeled Asn-Gly-Arg (NGR) Peptides for Imaging and Therapy. Mol. Imaging (IF 2.8) Pub Date : 2020-08-31 Liqin Zhu,Zhikai Ding,Xingliang Li,Hongyuan Wei,Yue Chen
Asn-Gly-Arg (NGR) motifs have vasculature-homing properties via interactions with the aminopeptidase N (CD13) expressed on tumor neovasculature. Numerous NGR peptides with different molecular scaffolds have been exploited for targeted delivery of different compounds for imaging and therapy. When conjugated with NGR, complexes recognize the CD13 receptor expressed on the tumor vasculature, which improves