Objective. To evaluate the diagnostic efficacy of MDA-MB-231 triple-negative breast cancer with ¹²⁵I-labeled pHLIP (Var7) by single-photon emission computed tomography/computed tomography (SPECT/CT) imaging. Methods. The binding fraction of [¹²⁵I]I-pHLIP (Var7) and MDA-MB-231 cells was measured at pH 7.4 and pH 6.0, and tumor-bearing mice were subjected to small-animal SPECT/CT imaging studies. Results. At , the binding fractions of [¹²⁵I]I-pHLIP (Var7) and MDA-MB-231 cells at 10 min, 40 min, 1 h, and 2 h were 1%, %, %, and %, respectively. At , there was no measured binding between [¹²⁵I]I-pHLIP (Var7) and MDA-MB-231 cells. Small-animal SPECT/CT imaging showed clearly visible tumors at 1 and 2 h after injection. Conclusions. [¹²⁵I]I-pHLIP (Var7) could bind to MDA-MB-231 cells in an acidic environment, and small-animal SPECT/CT imaging showed clear tumors at 1 and 2 h after probe injection.

中文翻译：

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[125I]I-pHLIP (Var7) 通过靶向肿瘤微环境对 MDA-MB-231 三阴性乳腺癌小鼠模型进行 SPECT/CT 成像的实验研究

客观。通过单光子发射计算机断层扫描/计算机断层扫描 (SPECT/CT) 成像评估 MDA-MB-231 三阴性乳腺癌与^{125 I 标记的 pHLIP (Var7) 的诊断效果。}方法。^{在 pH 7.4 和 pH 6.0 下测量 [ 125} I]I-pHLIP (Var7) 和 MDA-MB-231 细胞的结合分数，并对荷瘤小鼠进行小动物 SPECT/CT 成像研究。结果。在， [ ¹²⁵ I]I-pHLIP (Var7) 和 MDA-MB-231 细胞在 10 min、40 min、1 h 和 2 h 的结合分数为 1%,%,％， 和％， 分别。在， ^{[ 125} I]I-pHLIP (Var7) 和 MDA-MB-231 细胞之间没有测量到的结合小动物 SPECT/CT 成像在注射后 1 和 2 小时显示清晰可见的肿瘤。结论。[ ¹²⁵ I]I-pHLIP (Var7) 在酸性环境中可与 MDA-MB-231 细胞结合，小动物 SPECT/CT 成像在探针注射后 1 小时和 2 小时显示清晰的肿瘤。