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TSHR Variant Screening and Phenotype Analysis in 367 Chinese Patients With Congenital Hypothyroidism. Ann. Lab. Med. (IF 4.9) Pub Date : 2024-03-04 Hai-Yang Zhang, Feng-Yao Wu, Xue-Song Li, Ping-Hui Tu, Cao-Xu Zhang, Rui-Meng Yang, Ren-Jie Cui, Chen-Yang Wu, Ya Fang, Liu Yang, Huai-Dong Song, Shuang-Xia Zhao
Genetic defects in the human thyroid-stimulating hormone (TSH) receptor (TSHR) gene can cause congenital hypothyroidism (CH). However, the biological functions and comprehensive genotype-phenotype relationships for most TSHR variants associated with CH remain unexplored. We aimed to identify TSHR variants in Chinese patients with CH, analyze the functions of the variants, and explore the relationships
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Comparison of Optical Genome Mapping With Conventional Diagnostic Methods for Structural Variant Detection in Hematologic Malignancies. Ann. Lab. Med. (IF 4.9) Pub Date : 2024-03-04 Yeeun Shim, Yu-Kyung Koo, Saeam Shin, Seung-Tae Lee, Kyung-A Lee, Jong Rak Choi
Structural variants (SVs) are currently analyzed using a combination of conventional methods; however, this approach has limitations. Optical genome mapping (OGM), an emerging technology for detecting SVs using a single-molecule strategy, has the potential to replace conventional methods. We compared OGM with conventional diagnostic methods for detecting SVs in various hematologic malignancies.
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Clonal Distribution and Its Association With the Carbapenem Resistance Mechanisms of Carbapenem-Non-Susceptible Pseudomonas aeruginosa Isolates From Korean Hospitals. Ann. Lab. Med. (IF 4.9) Pub Date : 2024-03-04 Nayeong Kim, Seo Yeon Ko, Seong Yong Park, Seong Yeob Kim, Da Eun Lee, Ki Tae Kwon, Yu Kyung Kim, Je Chul Lee
Carbapenem resistance in Pseudomonas aeruginosa is a serious global health problem. We investigated the clonal distribution and its association with the carbapenem resistance mechanisms of carbapenem-non-susceptible P. aeruginosa isolates from three Korean hospitals.
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Guide to Rho(D) Immune Globulin in Women With Molecularly Defined Asian-type DEL (c.1227G>A). Ann. Lab. Med. (IF 4.9) Pub Date : 2024-02-22 In Hwa Jeong, SooHo Yu, Tae Yeul Kim, Soo-Young Oh, Duck Cho
Rh hemolytic disease of the fetus and newborn is a potential risk for D-negative mothers who produce anti-D during pregnancy, which can lead to morbidity and mortality in subsequent pregnancies. To prevent this hemolytic disease, Rho(D) immune globulin (RhIG) is generally administered to D-negative mothers without anti-D at 28 weeks of gestation and shortly after delivery. However, current guidelines
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Evaluating the TaqMan Jra-Genotyping Method for Rapidly Predicting the Presence of Anti-Jra Antibodies. Ann. Lab. Med. (IF 4.9) Pub Date : 2024-02-20 Yu-Kyung Koo, Soon Sung Kwon, Eun Jung Suh, Na Hyeong Kim, Hyun Kyung Kim, Youn Keong Cho, Seung Jun Choi, Sinyoung Kim, Kyung-A Lee
The Jra antigen is a high-prevalence red blood cell (RBC) antigen. Reports on cases of fatal hemolytic disease of the fetus and newborn and acute hemolytic transfusion reactions suggest that antibodies against Jra (anti-Jra) have potential clinical significance. Identifying anti-Jra is challenging owing to a lack of commercially available antisera. We developed an alternative approach to rapidly predict
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Educational Outcomes and Perception Changes in Medical Students After Visiting a Blood Donation Center. Ann. Lab. Med. (IF 4.9) Pub Date : 2024-02-20 Junseo Lee, Seryeong Kim, Sun Young Jeong, Seug Yun Yoon, Namsu Lee, Jong-Ho Won, Jeong Won Shin, Soon Hyo Kwon, Min-Young Lee, Kyoung Ha Kim
Educating primary care physicians about blood donation and transfusion is critical. The Division of Hematology and Oncology at Soonchunhyang University Seoul Hospital in Korea introduced an on-site educational program termed the Blood Donation Center Visiting Program in the clerkship education for final-year medical students. We evaluated the educational outcomes and changes in perception among medical
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Comparative Analysis of AB vs. ABO-specific Plasma for Desensitization in Blood Group O Recipients: An In Vitro Study. Ann. Lab. Med. (IF 4.9) Pub Date : 2024-01-19 Young Ae Lim
Neutralizing capacity measurement (NCM) of soluble ABH substances (SAS) in plasma was assessed to guide the selection of the appropriate ABO group of fresh-frozen plasma (FFP) for plasma exchange (PE) in blood group O recipients with ABO-incompatible transplantations. Neutralizing capacity was assessed by measuring anti-A and/or anti-B titers in samples comprising one unit of O FFP and 10 O EDTA plasma
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Comparison of Measurable Residual Disease in Pediatric B-Lymphoblastic Leukemia Using Multiparametric Flow Cytometry and Next-Generation Sequencing. Ann. Lab. Med. (IF 4.9) Pub Date : 2024-01-19 Sang Mee Hwang, Inseong Oh, Seok Ryun Kwon, Jee-Soo Lee, Moon-Woo Seong
Measurable residual disease (MRD) testing, a standard procedure in B-lymphoblastic leukemia (B-ALL) diagnostics, is assessed using multiparametric flow cytometry (MFC) and next-generation sequencing (NGS) analysis of immunoglobulin gene rearrangements. We evaluated the concordance between eight-color, two-tube MFC-MRD the LymphoTrack NGS-MRD assays using 139 follow-up samples from 54 pediatric patients
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Clinical Practice Guideline for Blood-Based Circulating Tumor DNA Assays. Ann. Lab. Med. (IF 4.9) Pub Date : 2024-01-15 Jee-Soo Lee, Eun Hye Cho, Boram Kim, Jinyoung Hong, Young-Gon Kim, Yoonjung Kim, Ja-Hyun Jang, Seung-Tae Lee, Sun-Young Kong, Woochang Lee, Saeam Shin, Eun Young Song
Circulating tumor DNA (ctDNA) has emerged as a promising tool for various clinical applications, including early diagnosis, therapeutic target identification, treatment response monitoring, prognosis evaluation, and minimal residual disease detection. Consequently, ctDNA assays have been incorporated into clinical practice. In this review, we offer an in-depth exploration of the clinical implementation
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Optimization of a Protocol for Isolating Cell-free DNA from Cerebrospinal Fluid. Ann. Lab. Med. (IF 4.9) Pub Date : 2023-12-28 Ho Hyun Song, Hyeran Park, Doohwan Cho, Hae In Bang, Hyuk-Jin Oh, Jieun Kim
A standardized protocol for the isolation of cell-free DNA (cfDNA) from cerebrospinal fluid (CSF) is lacking. Therefore, we established a cfDNA isolation protocol optimized for clinical CSF specimens, integrating acceptable modifications and using artificial CSF generated from remnant CSF spiked with reference cell-free tumor DNA (ctDNA). We compared the isolation yields of in vitro diagnostic (IVD)-certified
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Current Status of Flow Cytometric Immunophenotyping of Hematolymphoid Neoplasms in Korea. Ann. Lab. Med. (IF 4.9) Pub Date : 2023-12-26 Mikyoung Park, Jihyang Lim, Ari Ahn, Eun-Jee Oh, Jaewoo Song, Kyeong-Hee Kim, Jin-Yeong Han, Hyun-Woo Choi, Joo-Heon Park, Kyung-Hwa Shin, Hyerim Kim, Miyoung Kim, Sang-Hyun Hwang, Hyun-Young Kim, Duck Cho, Eun-Suk Kang
Flow cytometric immunophenotyping of hematolymphoid neoplasms (FCI-HLN) is essential for diagnosis, classification, and minimal residual disease (MRD) monitoring. FCI-HLN is typically performed using in-house protocols, raising the need for standardization. Therefore, we surveyed the current status of FCI-HLN in Korea to obtain fundamental data for quality improvement and standardization.
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NUP214 Rearrangements in Leukemia Patients: A Case Series From a Single Institution. Ann. Lab. Med. (IF 4.9) Pub Date : 2023-12-26 Yu Jeong Choi, Young Kyu Min, Seung-Tae Lee, Jong Rak Choi, Saeam Shin
The three best-known NUP214 rearrangements found in leukemia (SET::NUP214, NUP214::ABL1, and DEK::NUP214) are associated with treatment resistance and poor prognosis. Mouse experiments have shown that NUP214 rearrangements alone are insufficient for leukemogenesis; therefore, the identification of concurrent mutations is important for accurate assessment and tailored patient management. Here, we characterized
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Optimizing the Hospital Blood Bank Stock in Korea: a Comparative Analysis of the Uniform 5-Day Stock Index and a Novel Blood Stock Index. Ann. Lab. Med. (IF 4.9) Pub Date : 2023-12-15 Seo-Jin Park, Young Ae Lim, Kyung-Hee Kim
Maintaining optimal blood inventory levels in hospitals is important to prevent blood shortage and wastage. We aimed to provide an efficient blood inventory management strategy for hospital blood banks nation-wide by comparing the current use of 5-day issuable stock (IS) with Lim's IS as a novel target IS.
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Performance Evaluation of the Roche Cobas 5800 HBV and HCV Tests: Comparison of the 200 and 500 μL Protocols. Ann. Lab. Med. (IF 4.9) Pub Date : 2023-12-15 Seri Jeong, Su Kyung Lee, Eun-Jung Cho, Han-Sung Kim, Young Kyung Lee, Jae-Seok Kim, Wonkeun Song, Hyun Soo Kim
Clinical management of patients infected with hepatitis B virus (HBV) or hepatitis C virus (HCV) relies on the viral load (VL). The Cobas 5800 system (Roche Diagnostics) can determine VLs in 200 and 500 μL samples, but the performance of each protocol has not been compared. We evaluated the performance of both protocols for the HBV and HCV tests.
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Evaluation of Coefficients of Variation for Clinical Chemistry Analytes Based on Internal Quality Control Data Across 5,425 Laboratories in China From 2013 to 2022. Ann. Lab. Med. (IF 4.9) Pub Date : 2023-11-28 Wei Wang,Zhixin Zhang,Chuanbao Zhang,Haijian Zhao,Shuai Yuan,Jiali Liu,Na Dong,Zhiguo Wang,Fengfeng Kang
Background Clinical chemistry tests are most widely used in clinical laboratories, and diverse measurement systems for these analyses are available in China. We evaluated the imprecision of clinical chemistry measurement systems based on internal QC (IQC) data. Methods IQC data for 27 general chemistry analytes were collected in February each year from 2013 to 2022. Four performance specifications
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Exploring Renal Function Assessment: Creatinine, Cystatin C, and Estimated Glomerular Filtration Rate Focused on the European Kidney Function Consortium Equation. Ann. Lab. Med. (IF 4.9) Pub Date : 2023-11-01 Hans Pottel,Pierre Delanaye,Etienne Cavalier
Serum creatinine and serum cystatin C are the most widely used renal biomarkers for calculating the estimated glomerular filtration rate (eGFR), which is used to estimate the severity of kidney damage. In this review, we present the basic characteristics of these biomarkers, their advantages and disadvantages, some basic history, and current laboratory measurement practices with state-of-the-art methodology
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Primary Hyperoxaluria Screening and Monitoring: Quantitative Measurement of Plasma Oxalate by Gas Chromatography-Mass Spectrometry With High Sensitivity. Ann. Lab. Med. (IF 4.9) Pub Date : 2023-10-31 Mehrdad Yazdanpanah,Jessie Cameron,Chandra Chappel,Libin Yuan
Background Plasma oxalate measurements can be used for the screening and therapeutic monitoring of primary hyperoxaluria. We developed a gas chromatography-mass spectrometry (GC-MS) assay for plasma oxalate measurements with high sensitivity and suitable testing volumes for pediatric populations. Methods Plasma oxalate was extracted, derivatized, and analyzed by GC-MS. We measured the ion at m/z 261
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Evaluation of a Modified Protocol for the SepsiPrep Kit for Direct Identification and Antimicrobial Susceptibility Testing From Positive Blood Culture Using BACTEC Plus and BacT/Alert Blood Culture Bottles. Ann. Lab. Med. (IF 4.9) Pub Date : 2023-10-30 In Young Yoo,Sung Il Ha,Hee Jae Huh,Tae Yeul Kim,Hyang Jin Shim,Hyeyoung Lee,Jayoung Kim,Nam Yong Lee,Yeon-Joon Park
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Identification of Potential Genomic Alterations Using Pan-Cancer Cell-Free DNA Next-Generation Sequencing in Patients With Gastric Cancer. Ann. Lab. Med. (IF 4.9) Pub Date : 2023-10-30 Boyeon Kim,Yoonjung Kim,Jae Yong Cho,Kyung-A Lee
Background Molecular cancer profiling may lead to appropriate trials for molecularly targeted therapies. Cell-free DNA (cfDNA) is a promising diagnostic and/or prognostic biomarker in gastric cancer (GC). We characterized somatic genomic alterations in cfDNA of patients with GC. Methods Medical records and cfDNA data of 81 patients diagnosed as having GC were reviewed. Forty-nine and 32 patients were
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Head-to-Head Comparison of Nine Assays for the Detection of Anti-Echinococcus Antibodies: A Retrospective Evaluation. Ann. Lab. Med. (IF 4.9) Pub Date : 2023-10-26 Carolina Mattwich,Kristina Huber,Gisela Bretzel,Sebastian Suerbaum,Andreas Wieser,Karl Dichtl
Background Echinococcosis is a neglected tropical disease that is severely underdiagnosed in resource-limited settings. In developed countries, diagnosing echinococcosis is challenging, and reliable serological assays are urgently needed. In the Central European Alps, EM is more common than EG; however, data on the diagnostic performance of assays for EM cases are scarce. We evaluated the suitability
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The Use of Bone-Turnover Markers in Asia-Pacific Populations. Ann. Lab. Med. (IF 4.9) Pub Date : 2023-10-23 Samuel Vasikaran,Subashini C Thambiah,Rui Zhen Tan,Tze Ping Loh,
Bone-turnover marker (BTM) measurements in the blood or urine reflect the bone-remodeling rate and may be useful for studying and clinically managing metabolic bone diseases. Substantial evidence supporting the diagnostic use of BTMs has accumulated in recent years, together with the publication of several guidelines. Most clinical trials and observational and reference-interval studies have been performed
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A Liquid Chromatography-Tandem Mass Spectrometry Method for Simultaneously Determining Meropenem and Linezolid in Blood and Cerebrospinal Fluid. Ann. Lab. Med. (IF 4.9) Pub Date : 2023-10-23 Joanna Berska,Jolanta Bugajska,Krystyna Sztefko
Antibiotic therapy requires appropriate dosage of drugs for effective treatment. Too low antibiotic concentrations may lead to treatment failure and the development of resistant pathogens, whereas overdosing may cause neurological side effects or hemolytic diseases. Meropenem and linezolid are used only in the treatment of serious infections or when other antibiotics are no longer effective as well
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Concurrent Pleural and Pericardial Involvement in a Patient With De Novo Pure Erythroid Leukemia. Ann. Lab. Med. (IF 4.9) Pub Date : 2023-10-16 Yun Zhang,Kechao Li,Xue Li,Hui Wang,Ting Li,Fang Long
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Re-evaluation of a Fibrillin-1 Gene Variant of Uncertain Significance Using the ClinGen Guidelines. Ann. Lab. Med. (IF 4.9) Pub Date : 2023-10-16 Seo Wan Kim,Boyeon Kim,Yoonjung Kim,Kyung-A Lee
Background Marfan syndrome (MFS) is caused by fibrillin-1 gene (FBN1) variants. Mutational hotspots and/or well-established critical functional domains of FBN1 include cysteine residues, calcium-binding consensus sequences, and amino acids related to interdomain packaging. Previous guidelines for variant interpretation do not reflect the features of genes or related diseases. Using the Clinical Genome
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Reverse Transcription-PCR-based Sanger Sequencing-confirmed Exon-skipping Effect of a Novel GEN1 Intronic Variant (c.1408+4A>G). Ann. Lab. Med. (IF 4.9) Pub Date : 2023-10-16 Jiyeon Kim,Joonsang Yu,Seunghoo Lee,Sollip Kim,In-Seob Lee,Woochang Lee,Sail Chun
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Predictive Performance of Neutrophil Gelatinase Associated Lipocalin, Liver Type Fatty Acid Binding Protein, and Cystatin C for Acute Kidney Injury and Mortality in Severely Ill Patients. Ann. Lab. Med. (IF 4.9) Pub Date : 2023-09-26 Ayu Asakage, Shiro Ishihara, Louis Boutin, François Dépret, Takeshi Sugaya, Naoki Sato, Etienne Gayat, Alexandre Mebazaa, Benjamin Deniau
Acute kidney injury (AKI) is a common condition in severely ill patients associated with poor outcomes. We assessed the associations between urinary neutrophil gelatinase-associated lipocalin (uNGAL), urinary liver-type fatty acid-binding protein (uLFABP), and urinary cystatin C (uCysC) concentrations and patient outcomes.
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Identification of Two Novel ANKRD11 Mutations: Highlighting Incomplete Penetrance in KBG Syndrome. Ann. Lab. Med. (IF 4.9) Pub Date : 2023-09-04 Nada Amllal,Siham Chafai Elalaoui,Maria Zerkaoui,Amal Chiguer,Lamia Afif,Amal Thimou Izgua,Abdelaziz Sefiani,Jaber Lyahyai
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Effectiveness of Unified Computerized Reporting of Point-of-Care Glucose Meter Test. Ann. Lab. Med. (IF 4.9) Pub Date : 2023-09-04 Sooin Choi,Dughyun Choi,Soo Jeong Choi,Jin Kuk Kim,You Kyoung Lee,Yong-Wha Lee
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In Vitro Activity of Benzimidazole (SPR719) Against Clinical Isolates of Nontuberculous Mycobacteria With and Without Clarithromycin or Amikacin Resistance. Ann. Lab. Med. (IF 4.9) Pub Date : 2023-09-04 Dae Hun Kim,Sungmin Zo,Su-Young Kim,Byung Woo Jhun
Limited data are available regarding the in vitro activity of SPR719, a derivative of benzimidazole, against diverse nontuberculous mycobacteria (NTM) species. We investigated the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of SPR719 against clinical NTM isolates, including clarithromycin- and amikacin-resistant strains. NTM isolates were obtained from patients
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The First Korean Hemoglobinopathy With Unique Hemoglobin Electrophoresis Results Diagnosed as Hemoglobin Boras. Ann. Lab. Med. (IF 4.9) Pub Date : 2023-09-04 Jeongyun Bae,Won Kee Ahn,Jaehyeok Jang,Hanmil Jang,Hyein Kang,John Hoon Rim,Seung Min Hahn,Jung Woo Han,Chuhl Joo Lyu,Jong-Baeck Lim
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Skeletal Muscle Mass Measurement via Bioelectrical Impedance Analysis as an Aid to Screen for Chronic Kidney Disease in Routine Health Checkups. Ann. Lab. Med. (IF 4.9) Pub Date : 2023-09-04 Seunghoo Lee,Sangpil Yoon,Sollip Kim,Woochang Lee,Sail Chun,Won-Ki Min
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Preventing Blood Transfusion Errors: How Personal Digital Assistants Can Improve Patient Safety. Ann. Lab. Med. (IF 4.9) Pub Date : 2023-09-04 Eunhui Ji,Kae Lyang Koo,Hyun Ki Min,Sun Min Lee,Seung-Hwan Oh,Hyun Ji Lee
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Clinical Usefulness of a Cell-based Assay for Detecting Myelin Oligodendrocyte Glycoprotein Antibodies in Central Nervous System Inflammatory Disorders. Ann. Lab. Med. (IF 4.9) Pub Date : 2023-09-04 Jin Myoung Seok,Patrick Waters,Mi Young Jeon,Hye Lim Lee,Seol-Hee Baek,Jin-Sung Park,Sa-Yoon Kang,Ohyun Kwon,Jeeyoung Oh,Byung-Jo Kim,Kyung-Ah Park,Sei Yeul Oh,Byoung Joon Kim,Ju-Hong Min
Background The clinical implications of myelin oligodendrocyte glycoprotein autoantibodies (MOG-Abs) are increasing. Establishing MOG-Ab assays is essential for effectively treating patients with MOG-Abs. We established an in-house cell-based assay (CBA) to detect MOG-Abs to identify correlations with patients' clinical characteristics. Methods We established the CBA using HEK 293 cells transiently
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Prediction of Mid-term Platelet Transfusion in Stable Trauma Patients Using Rotational Thromboelastometry. Ann. Lab. Med. (IF 4.9) Pub Date : 2023-09-04 Ha Jin Lim,Hyunseok Jang,Naa Lee,Euisung Jeong,Yunchul Park,Younggoun Jo,Jungchul Kim,Young Eun Lee,Hyun-Jung Choi,Seung-Jung Kee,Jong Hee Shin,Myung Geun Shin
Background Rotational thromboelastometry (ROTEM; TEM International GmbH, Munich, Germany) is a global coagulation test that guides evidence-based platelet transfusion in trauma patients. We evaluated ROTEM parameters for predicting mid-term (five days) platelet transfusion in trauma patients. Methods Maximum clot firmness and clot amplitudes after 5, 10, and 15 mins (A5, A10, and A15, respectively)
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Association Between Low Anti-spike Antibody Levels After the Third Dose of SARS-CoV-2 Vaccination and Hospitalization due to Symptomatic Breakthrough Infection in Kidney Transplant Recipients. Ann. Lab. Med. (IF 4.9) Pub Date : 2023-09-04 Ahram Han,Sangil Min,Eun-Ah Jo,Hajeong Lee,Yong Chul Kim,Seung Seok Han,Hee Gyung Kang,Yo Han Ahn,Inseong Oh,Eun Young Song,Jongwon Ha
Background Whether anti-severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibody levels post-third coronavirus disease (COVID-19) vaccination correlate with worse outcomes due to breakthrough infection is unclear. We evaluated the association between anti-SARS-CoV-2 antibody levels and symptomatic breakthrough infection or hospitalization during the Omicron surge in kidney transplant recipients
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Comparative Genomic Analysis of Staphylococcal Cassette Chromosome mec Type V Staphylococcus aureus Strains and Estimation of the Emergence of SCCmec V Clinical Isolates in Korea. Ann. Lab. Med. (IF 4.9) Pub Date : 2023-09-04 Takashi Takahashi,Hyaekang Kim,Han-Sung Kim,Hyun Soo Kim,Wonkeun Song,Jae-Seok Kim
Background Staphylococcal cassette chromosome mec type V (SCCmec V) methicillin-resistant Staphylococcus aureus (MRSA) has been recovered from patients and livestock. Using comparative genomic analyses, we evaluated the phylogenetic emergence of SCCmec V after transmission from overseas donor strains to Korean recipient strains. Methods Sixty-three complete MRSA SCCmec V genomes (including six Korean
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Changing Genotypic Distribution, Antimicrobial Susceptibilities, and Risk Factors of Urinary Tract Infection Caused by Carbapenemase-Producing Pseudomonas aeruginosa. Ann. Lab. Med. (IF 4.9) Pub Date : 2023-09-04 Seri Jeong,Kibum Jeon,Nuri Lee,Min-Jeong Park,Wonkeun Song
Background Carbapenem-resistant Pseudomonas aeruginosa (CrPA) is a leading cause of healthcare-associated urinary tract infections (UTIs). Carbapenemase production is an important mechanism that significantly alters the efficacy of frequently used anti-pseudomonal agents. Reporting the current genotypic distribution of carbapenemase-producing P. aeruginosa (CPPA) isolates in relation to antimicrobial
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Bias in Laboratory Medicine: The Dark Side of the Moon. Ann. Lab. Med. (IF 4.9) Pub Date : 2023-09-04 Abdurrahman Coskun
Physicians increasingly use laboratory-produced information for disease diagnosis, patient monitoring, treatment planning, and evaluations of treatment effectiveness. Bias is the systematic deviation of laboratory test results from the actual value, which can cause misdiagnosis or misestimation of disease prognosis and increase healthcare costs. Properly estimating and treating bias can help to reduce
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Evaluation of Group Genetic Counseling Sessions via a Metaverse-based Application. Ann. Lab. Med. (IF 4.9) Pub Date : 2023-09-04 Boeun Yoo,Arang Kim,Hye Sung Moon,Min-Kyung So,Tae-Dong Jeong,Kyoung Eun Lee,Byung-In Moon,Jungwon Huh
Background Group genetic counseling has been implemented to meet growing demand. A metaverse platform, in which a society is built and activities are carried out in the virtual world, has not yet been implemented in group genetic counseling. We investigated whether a metaverse platform could be an alternative service-delivery model for group genetic counseling. Methods Participants (N=131) were divided
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Ionized Magnesium Correlates With Total Blood Magnesium in Pediatric Patients Following Kidney Transplant. Ann. Lab. Med. (IF 4.9) Pub Date : 2023-09-04 Denise C Hasson,Shruthi Mohan,James E Rose,Kyle A Merrill,Stuart L Goldstein,Stefanie W Benoit,Charles D Varnell
Background Abnormal serum magnesium (Mg) concentrations are common and associated with worse mortality in kidney-transplant recipients. Many kidney and transplant-related factors affect Mg homeostasis. The concentration of the active form, ionized Mg (iMg), is not measured clinically, and total Mg (tMg) and iMg correlations have conflicted. We hypothesized that iMg and tMg concentrations show poor
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Challenges of Carbapenem-resistant Pseudomonas aeruginosa in Infection Control and Antibiotic Management. Ann. Lab. Med. (IF 4.9) Pub Date : 2023-09-04 Young Jin Kim,Hee Jae Huh,Heungsup Sung
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New Insights Into SARS-CoV-2-specific Antibody Levels in Kidney Transplantation Recipients After Three Vaccination Doses. Ann. Lab. Med. (IF 4.9) Pub Date : 2023-09-04 Hyunhye Kang,Eun-Jee Oh
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Comparison of Direct and Extraction Immunoassay Methods With Liquid Chromatography-Tandem Mass Spectrometry Measurement of Urinary Free Cortisol for the Diagnosis of Cushing's Syndrome. Ann. Lab. Med. (IF 4.9) Pub Date : 2023-09-04 Danni Mu,Jiadan Fang,Songlin Yu,Yichen Ma,Jin Cheng,Yingying Hu,Ailing Song,Fang Zhao,Qi Zhang,Zhihong Qi,Kui Zhang,Liangyu Xia,Ling Qiu,Huijuan Zhu,Xinqi Cheng
Background Twenty-four-hour urinary free cortisol (UFC) measurement is the initial diagnostic test for Cushing's syndrome (CS). We compared UFC determination by both direct and extraction immunoassays using Abbott Architect, Siemens Atellica Solution, and Beckman DxI800 with liquid chromatography-tandem mass spectrometry (LC-MS/MS). In addition, we evaluated the value of 24-hr UFC measured by six methods
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ABO Blood Group Antigen Changes in Acute Myeloid Leukemia and No Significant Association With RUNX1 and GATA2 Somatic Variants. Ann. Lab. Med. (IF 4.9) Pub Date : 2023-06-30 In Hwa Jeong,Ji Young Seo,Sooin Choi,Hyun-Young Kim,Duck Cho
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Variant Allele Frequency of Pseudogene-Related Variants in Short-read Next-Generation Sequencing Data May Mislead Genetic Diagnosis: A Case of Shwachman-Diamond Syndrome. Ann. Lab. Med. (IF 4.9) Pub Date : 2023-06-30 Heerah Lee,Jung Ae Lee,Hyesu Lee,Jee-Soo Lee,Jung Min Ko,Man Jin Kim,Moon-Woo Seong
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No Observed Interference by Nucleated Red Blood Cells in Total Bilirubin Measurement by Point-of-Care Testing: Six Years of Experience. Ann. Lab. Med. (IF 4.9) Pub Date : 2023-06-30 Estéfani Martínez Chávez,Paloma Oliver,Antonio Buño,Pilar Fernández-Calle
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Estradiol Testing in the Exploration of Early Onset Puberty: Comparison of RIA and Chemiluminescence Immunoassay. Ann. Lab. Med. (IF 4.9) Pub Date : 2023-06-30 Justine Blin,Clarisse Hochman,Nolwenn Graveline,Damien Masson,Sabine Baron,Kalyane Bach-Ngohou
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New Issues With Neutrophil Gelatinase-associated Lipocalin in Acute Kidney Injury. Ann. Lab. Med. (IF 4.9) Pub Date : 2023-06-30 Sun Young Cho,Mina Hur
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Improving the Work Efficiency of Therapeutic Drug Monitoring Reports Through the Establishment of an Automated Data Transfer System. Ann. Lab. Med. (IF 4.9) Pub Date : 2023-06-30 Seunghoo Lee,Sangpil Yoon,Hyun-Ki Kim,Sollip Kim,Woochang Lee,Sail Chun,Won-Ki Min
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Validation of High-sensitivity Flow Cytometry for Reliable Immune Cell Analysis in Real-world Laboratory Settings. Ann. Lab. Med. (IF 4.9) Pub Date : 2023-06-30 Hyunji Kim, Sung Shin, Sang-Hyun Hwang, Miyoung Kim, Young-Uk Cho, Seongsoo Jang
The adoption of high-sensitivity flow cytometry (HSFC) in routine laboratory settings has been slow owing to concerns regarding the reliability and reproducibility of results. Validation is an essential prerequisite for conducting assays, and implementing the CLSI guidelines has been confusing, primarily because many aspects are not yet established. We aimed to validate an HSFC protocol for detecting
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Cost-Effectiveness Analysis of Three Diagnostic Strategies for the Detection of EGFR Mutation in Advanced Non-Small Cell Lung Cancer. Ann. Lab. Med. (IF 4.9) Pub Date : 2023-06-30 Sun Mi Cho, Hye Sun Lee, Soyoung Jeon, Yoonjung Kim, Sun-Young Kong, Jin Kyung Lee, Kyung-A Lee
In non-small cell lung cancer (NSCLC), epidermal growth factor receptor (EGFR) mutation testing of tumor tissue should be conducted at diagnosis. Alternatively, circulating tumor DNA can be used to detect EGFR mutation. We compared the cost and clinical effect of three strategies according to the application of the EGFR test.
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Molecular and Clinical Features of Fluconazole Non-susceptible Candida albicans Bloodstream Isolates Recovered in Korean Multicenter Surveillance Studies. Ann. Lab. Med. (IF 4.9) Pub Date : 2023-06-30 Min Ji Choi, Yong Jun Kwon, Seung A Byun, Mi-Na Kim, Wee Gyo Lee, Jaehyeon Lee, Dongeun Yong, Chulhun L Chang, Eun Jeong Won, Soo Hyun Kim, Seung Yeob Lee, Jong Hee Shin
Acquired fluconazole resistance (FR) in bloodstream infection (BSI) isolates of Candida albicans is rare. We investigated the FR mechanisms and clinical features of 14 fluconazole non-susceptible (FNS; FR and fluconazole-susceptible dose-dependent) BSI isolates of C. albicans recovered from Korean multicenter surveillance studies during 2006-2021. Mutations causing amino acid substitutions (AASs) in
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Effect of Two Cystatin C Reagents and Four Equations on Glomerular Filtration Rate Estimations After Standardization. Ann. Lab. Med. (IF 4.9) Pub Date : 2023-06-30 Hyun-Seung Lee, Go-Eun Bae, Jung Eun Lee, Hyung-Doo Park
Serum cystatin C (cysC), which is less affected by sex, race, and muscle mass than creatinine, is a useful biomarker of the estimated glomerular filtration rate (eGFR). The standardization of cysC measurements remains controversial, although a certified reference material (ERM-DA471/IFCC) is available. Moreover, the effect of combinations of cysC reagents and equations for eGFR is unclear.
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Comparison of the New VITEK MS PRIME System with the Matrix-Assisted Laser Desorption Ionization Biotyper Microflex LT for the Identification of Microorganisms. Ann. Lab. Med. (IF 4.9) Pub Date : 2023-06-30 Patrick Grohs, Elodie Remaud, Cybill Lath, Kim Vuong, Marie-Lize Parolini, Eric Dannaoui, Isabelle Podglajen
Matrix-assisted laser desorption ionization (MALDI) is the cornerstone of bacterial identification. The performance of a new MALDI time-of-flight mass spectrometry VITEK MS PRIME (VMS-P) system was compared with that of the MALDI Biotyper Microflex LT (MBT) system, which is routinely used in our laboratory.
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Clot Waveform Analysis for Hemostatic Abnormalities. Ann. Lab. Med. (IF 4.9) Pub Date : 2023-06-30 Hideo Wada, Katsuya Shiraki, Takeshi Matsumoto, Hideto Shimpo, Motomu Shimaoka
Clot waveform analysis (CWA) observes changes in transparency in a plasma sample based on clotting tests such as activated partial thromboplastin time (APTT), prothrombin time (PT), and thrombin time (TT). Evidence indicates that not only an abnormal waveform but also peak times and heights in derivative curves of CWA are useful for the evaluation of hemostatic abnormalities. Modified CWA, including
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Neutrophil Gelatinase-Associated Lipocalin Cutoff Value Selection and Acute Kidney Injury Classification System Determine Phenotype Allocation and Associated Outcomes. Ann. Lab. Med. (IF 4.9) Pub Date : 2023-06-30 Annemarie Albert, Sebastian Radtke, Louisa Blume, Rinaldo Bellomo, Michael Haase, Philipp Stieger, Ulrich Paul Hinkel, Rüdiger C Braun-Dullaeus, Christian Albert
We explored the extent to which neutrophil gelatinase-associated lipocalin (NGAL) cutoff value selection and the acute kidney injury (AKI) classification system determine clinical AKI-phenotype allocation and associated outcomes.
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Comparison of Four T-cell Assays and Two Binding Antibody Assays in SARS-CoV-2 Vaccinees With or Without Omicron Breakthrough Infection. Ann. Lab. Med. (IF 4.9) Pub Date : 2023-06-30 Yeon Ju Seo, Inseong Oh, Minjeong Nam, Sue Shin, Eun Youn Roh, Eun Young Song
Several T-cell response assays for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are available; however, their comparability and correlations with antibody responses remain unclear. We compared four SARS-CoV-2 T-cell response assays and two anti-SARS-CoV-2 spike antibody assays.
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Evaluation of Vancomycin Area Under the Concentration-Time Curve Predictive Performance Using Bayesian Modeling Software With and Without Peak Concentration: An Academic Hospital Experience for Adult Patients Without Renal Impairment. Ann. Lab. Med. (IF 4.9) Pub Date : 2023-06-30 Hyun-Ki Kim, Tae-Dong Jeong
The revised U.S. consensus guidelines on vancomycin therapeutic drug monitoring (TDM) recommend obtaining trough and peak samples to estimate the area under the concentration-time curve (AUC) using the Bayesian approach; however, the benefit of such two-point measurements has not been demonstrated in a clinical setting. We evaluated Bayesian predictive performance with and without peak concentration
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Soluble Suppression of Tumorigenicity-2 as a Candidate Prognostic Marker for Stroke: A Systematic Review. Ann. Lab. Med. (IF 4.9) Pub Date : 2023-06-30 Koohyar Ahmadzadeh, Shayan Roshdi Dizaji, Mohammad Balabandian, Hamzah Adel Ramawad, Mahmoud Yousefifard
Risk stratification of patients for incidence of stroke and its outcomes can aid in decision-making regarding treatment options and rehabilitative care. We systematically reviewed the literature to provide comprehensive evidence for the value of serum soluble suppression of tumorigenicity-2 (sST-2) in the prediction of stroke incidence and the evaluation of post-stroke outcomes.