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Metabolic and proteomic signatures differentiate inflammatory phenotypes from cancer and predict treatment response in patient sera Bioeng. Transl. Med. (IF 6.1) Pub Date : 2025-05-15 Gabriel Cutshaw, Elena V. Demidova, Philip Czyzewicz, Elizabeth Quam, Nicole Lorang, AL Warith AL Siyabi, Surinder Batra, Sanjeevani Arora, Rizia Bardhan
Tumors shift their metabolic needs to enable uncontrolled proliferation. Therefore, metabolic assessment of cancer patient sera provides a significant opportunity to noninvasively monitor disease progression and enable mechanistic understanding of the pathways that lead to response. Here, we show Raman spectroscopy (RS), a highly sensitive and label‐free analytical tool, is effective in metabolic profiling
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Synergizing photodynamic therapy and ethanol ablation: Light‐activatable sustained‐exposure ethanol injection technology for enhanced tumor ablation Bioeng. Transl. Med. (IF 6.1) Pub Date : 2025-05-15 Chen‐Hua Ma, Jeffrey Yang, John A. Quinlan, Kathryn McNaughton, Michele L. Kaluzienski, Tessa Hauser, Matthew F. Starost, Jenna L. Mueller, Huang‐Chiao Huang
Chemical ablative therapies offer effective alternatives for tumor treatment, particularly when surgical resection or heat‐based ablation therapies are unsuitable due to the tumor's stage, location, or extent. Photodynamic therapy (PDT), which involves delivering light‐activated, tumor‐killing photosensitizers, and percutaneous ethanol injection (PEI), which involves the direct injection of pure ethanol
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An in‐cell helicase reporter system for quantifying DDX3X and DDX3Y activities Bioeng. Transl. Med. (IF 6.1) Pub Date : 2025-05-15 Zhi Sheng Poh, James Chia Wei Tan, Brandon Han Siang Wong, Kottaiswamy Amuthavalli, Holy Kristanti, Suat Hoon Tan, Nicholas Francis Grigoropoulos, Navin Kumar Verma
Genome sequencing has identified numerous mutations in the DEAD‐box RNA helicases, DDX3X and DDX3Y, associated with cancer and other diseases, but monitoring of their functional consequences remains a challenge. Conventional helicase assays are laborious, often technically difficult, and are performed in cell‐free systems that do not address biologically relevant questions. Here, we developed an engineered
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Self‐assembly of verteporfin dimers into folate receptor‐targeted lipid nanoparticles for photodynamic therapy of ovarian cancer Bioeng. Transl. Med. (IF 6.1) Pub Date : 2025-05-14 Carla Arnau del Valle, Payal Srivastava, Kathryn McNaughton, Huang‐Chiao Huang
Photodynamic therapy (PDT) is a minimally invasive treatment that involves the administration of a light‐activatable drug followed by light activation of the lesion to produce reactive oxygen species that kill cancer cells. Visudyne®, a liposomal formulation of benzoporphyrin derivative (BPD) photosensitizer, is clinically approved for PDT of ocular diseases and is now being tested for PDT and imaging
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Characterizing a novel adult erythroid cell line for red blood cell manufacture Bioeng. Transl. Med. (IF 6.1) Pub Date : 2025-05-06 David Phillips, Marianne J. Ellis, Jan Frayne, Sandhya Moise
One way to address the growing shortage of donated blood required for routine and emergency transfusion is to mass‐manufacture red blood cells (RBCs) in vitro. However, numerous biological and bioprocessing challenges must be overcome to realize this goal. In addressing these challenges, a promising solution is the erythroid progenitor cell line, BEL‐A (Bristol Erythroid Line—Adult). Despite this,
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Early nanoparticle intervention preserves motor function following cervical spinal cord injury Bioeng. Transl. Med. (IF 6.1) Pub Date : 2025-04-30 Sarah E. Hocevar, Brian C. Ross, Yinghao Wang, Cecelia R. Crowther, Samantha R. Schwartz, Brain J. Cummings, Aileen J. Anderson, Lonnie D. Shea
Spinal cord injury (SCI) triggers an immediate influx of immune cells that secrete pro‐inflammatory cytokines and reactive oxygen species that cause tissue damage that is secondary to the initial physical trauma. We aim to reprogram these immune cells to promote a less inflammatory and more pro‐regenerative environment. Herein, we investigated the window in time during which poly(lactide‐co‐glycolide)
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Mitomycin C‐immobilized silver nanoparticle‐loaded polycaprolactone membrane for temporary scalp expansion after decompressive craniectomy to prevent wound infection Bioeng. Transl. Med. (IF 6.1) Pub Date : 2025-04-30 Kai‐Lun Liu, Ya‐Jyun Liang, Kuo‐Hsiang Hung, Yu‐Ning Chen, Feng‐Huei Lin
Malignant cerebral edema (MCE) represents a significant medical emergency characterized by unmanageable intracranial pressure (ICP), frequently arising as a consequence of traumatic brain injury (TBI) or ischemic stroke. Decompressive craniectomy (DC) is a prevalent surgical procedure employed to mitigate elevated ICP by excising a segment of the skull to enhance intracranial volume. Nevertheless,
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In vivo immunogenicity assessment of a multiepitope‐displayed phage vaccine against Brucella species infection in BALB/c mice Bioeng. Transl. Med. (IF 6.1) Pub Date : 2025-04-26 Mahmood Fadaie, Mahsa Esmaeilifallah, Anis Khalafiyan, Ilnaz Rahimmanesh, Seyed Hossein Hejazi, Hossein Khanahmad
Bacteriophages are considered ideal vaccine platforms owing to their safety, intrinsic adjuvant properties, stability, and low‐cost production. One of the best strategies to prevent brucellosis in humans and animals is vaccination. For several years, researchers have dedicated their efforts to enhance the effectiveness and safety of the Brucella vaccine. This study was designed to evaluate the immunogenicity
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Bionic scaffolds with integrated structural components based on low‐temperature deposition manufacturing 3D printing technology for the treatment of meniscus defects Bioeng. Transl. Med. (IF 6.1) Pub Date : 2025-04-25 Shi Shen, Yujie Li, Mingxue Chen, Weimin Guo, Shuang Gao, Zengzeng Zhang, Naiqiang Zhuo, Shuyun Liu, Quanyi Guo
Tissue engineering provides a promising avenue for treating meniscus defects. In this study, a novel polycaprolactone (PCL)/collagen type I (COL I) meniscus scaffold was fabricated using low temperature deposition manufacturing (LDM) 3D printing technology. The scaffold had a ring and radial fiber structure, and its composition and structure were double bionic of the natural meniscus. In vitro experiments
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Cell therapies against brain tumors: Clinical development and emerging prospects Bioeng. Transl. Med. (IF 6.1) Pub Date : 2025-04-16 Tatsuya Fukuta, Suyog Shaha, Andres da Silva‐Candal, Zongmin Zhao, Samir Mitragotri
Malignant brain tumors, particularly glioblastoma multiforme (GBM), are aggressive and fatal cancers. The clinical efficacy of current standard‐of‐care treatments against brain tumors has been minimal, with no significant improvement over the past 30 years. Driven by the success of chimeric antigen receptor (CAR)‐T cells in the clinic for treating certain types of cancer, adoptive cell therapies have
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Engineered lung cell targeting and SLC7A11 siRNA expressing bacterial extracellular vesicles impair the progression of none‐small cell lung cancer Bioeng. Transl. Med. (IF 6.1) Pub Date : 2025-04-16 Xiao‐dan Wan, Xue‐liang Zhou, Jin‐long Liu, Hua Xu
Non‐small cell lung cancer (NSCLC) presents significant therapeutic challenges, often characterized by aggressive proliferation and metastasis. This study investigates the role of SLC7A11, a ferroptosis‐related gene, in NSCLC progression and the potential of engineered bacterial extracellular vesicles (BEVs) expressing SLC7A11‐targeting siRNA as a therapeutic strategy. Using TCGA and GEO databases
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Developing a process of lentivirus purification from recombinant fluids using peptide affinity ligands Bioeng. Transl. Med. (IF 6.1) Pub Date : 2025-04-08 Eduardo Barbieri, Gina N. Mollica, Sobhana A. Sripada, Shrirarjun Shastry, Yuxuan Wu, Arianna Minzoni, Will Smith, Elena Wuestenhagen, Annika Aldinger, Heiner Graalfs, Michael S. Crapanzano, Oliver Rammo, Michael M. Schulte, Michael A. Daniele, Stefano Menegatti
Lentiviral vectors (LVVs) are emerging as an enabling tool in gene and cell therapies, yet the toolkit for purifying them at scale is still immature. A pivoting moment in LVV isolation technology was marked by the introduction of affinity ligands for LVVs pseudo‐typed with Vesicular Stomatitis Virus G (VSV‐G) protein. Camelid antibody ligands were initially discovered and utilized to functionalize
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Optimizing design parameters of 3D‐printed poly‐4‐hydroxybutyrate nipple scaffolds for nipple reconstruction Bioeng. Transl. Med. (IF 6.1) Pub Date : 2025-04-07 Xue Dong, Sophia Salingaros, Timothy Butler, Skander Limem, Jason A. Spector
Nipple reconstruction in patients who undergo total mastectomy or nipple‐sparing mastectomy is currently limited by a consistent and significant loss of nipple projection over time, which can negatively affect patient satisfaction and quality of life. To address this issue, we have previously shown that 3D‐printed poly‐4‐hydroxybutyrate (P4HB) nipple‐shaped scaffolds promote long‐term maintenance of
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Spatial‐hindrance‐based pro‐Adalimumab prevents anti‐idiotypic antibody interference in pharmacokinetic and therapeutic efficacy Bioeng. Transl. Med. (IF 6.1) Pub Date : 2025-04-04 Bo‐Cheng Huang, Yu‐Tung Chen, Yun‐Chi Lu, Kai‐Wen Ho, Shih‐Ting Hong, Tzu‐Yi Liao, I‐Hsuan Wu, En‐Shuo Liu, Jun‐Min Liao, Fang‐Ming Chen, Chia‐Ching Li, Chih‐Hung Chuang, Chiao‐Yun Chen, Tian‐Lu Cheng
Adalimumab (Humira) represents a major advance in rheumatoid arthritis (RA) therapy. However, with long‐term administration of Adalimumab, anti‐idiotypic antibody (anti‐Id Ab) accelerates the Adalimumab clearance rate and reduces the therapeutic effect. To avoid the interference of anti‐Id Ab, we used an autologous hinge region as a spatial‐hindrance‐based Ab lock and connected it to the N‐terminal
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Machine learning‐based SERS serum detection platform for high‐sensitive and high‐throughput diagnosis of colorectal precancerous lesions Bioeng. Transl. Med. (IF 6.1) Pub Date : 2025-03-31 Qunshan Zhu, Gaoyang Chen, Lei Fu, Dawei Cao, Zhenguang Wang, Yan Yang, Wei Wei
Colorectal precancerous lesions (CRP) are early signs of cancer development, and early detection helps prevent progression to colorectal cancer (CRC), reducing incidence and mortality rates. This study developed a serum detection platform integrating surface‐enhanced Raman scattering (SERS) with machine learning (ML) for early detection of CRP. Specifically, a microarray chip with Au/SnO2 nanorope
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Efficacy and safety assessment of homotopical transplantation of iPSCs‐derived midbrain organoids into the substantia nigra of Parkinsonian rats Bioeng. Transl. Med. (IF 6.1) Pub Date : 2025-03-30 Xin Zheng, Jianwei Chen, Zhengzheng Huang, Youcheng Zhang, Liping Zhou
Current ectopic implantation has shown limited efficacy in promoting reinnervation of the nigrostriatal pathway, which is critically affected in Parkinson's disease (PD). Homotopic transplantation, on the other hand, may facilitate physiological cell rewiring of the basal ganglia, potentially improving PD symptoms. This study aimed to evaluate the efficacy and safety of homotopically engrafting human
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Key parameters for designing robust 2D and 3D spheroid models for in vitro atherosclerosis research Bioeng. Transl. Med. (IF 6.1) Pub Date : 2025-03-23 Ibukunoluwa Naiyeju, Stephanie Lehoux, Maryam Tabrizian
Atherosclerosis is a chronic, systemic, inflammatory disease associated with the build‐up of fatty deposits (“plaques”) in the arteries. A major global health burden, severe atherosclerosis progresses to ischemic heart disease, an underlying condition which can exacerbate the occurrence of fatal events such as heart attack and stroke. Over the past two decades, the use of in vitro models to study atherosclerotic
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Correction to “A stretchable, electroconductive tissue adhesive for the treatment of neural injury” Bioeng. Transl. Med. (IF 6.1) Pub Date : 2025-03-19
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Transport physics‐informed reinforcement learning agents deployed in standalone infusion pumps for managing multidrug delivery in critical care Bioeng. Transl. Med. (IF 6.1) Pub Date : 2025-03-18 V. Chandran Suja, A. L. H. S. Detry, N. M. Sims, D. E. Arney, S. Mitragotri, R. A. Peterfreund
Managing delivery of complex multidrug infusions in anesthesia and critical care presents a significant clinical challenge. Current practices relying on manual control of infusion pumps often result in unpredictable drug delivery profiles and dosing errors—key issues highlighted by the United States Food and Drug Administration (FDA). To address these issues, we introduce the SMART (synchronized‐pump
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Correction to “Drug cross‐linking electrospun fiber for effective infected wound healing” Bioeng. Transl. Med. (IF 6.1) Pub Date : 2025-03-17
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Paquinimod‐hydrogel hybrid microneedle array patch alleviates hypertrophic scar via inhibiting M1 polarization Bioeng. Transl. Med. (IF 6.1) Pub Date : 2025-03-15 Zihui Zhang, Peng Wang, Hengdeng Liu, Hanwen Wang, Miao Zhen, Xuefeng He, Suyue Gao, Juntao Xie, Julin Xie
Hypertrophic scar (HS) is one of the most common complications of skin injuries, with a lack of effective therapeutic approaches to date. Most current research has focused on the dysfunction of hypertrophic scar fibroblasts (HSFBs) and dermal vascular endothelial cells (HDVECs), neglecting the crucial role of the inflammatory microenvironment that causes them to be abnormal. In this study, we first
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Micrometer‐scale tPA beads amplify plasmin generation for enhanced thrombolytic therapy Bioeng. Transl. Med. (IF 6.1) Pub Date : 2025-03-12 Matthew J. Osmond, Fabrice Dabertrand, Nidia Quillinan, Enming J. Su, Daniel A. Lawrence, David W. M. Marr, Keith B. Neeves
Rapid restoration of blood flow is critical in treating acute ischemic stroke. Current thrombolytic therapies using tissue plasminogen activator (tPA) are limited by low recanalization rates and risks of off‐target bleeding. Here, we demonstrate that a remarkably simple adjustment—using micrometer‐scale rather than sub‐micrometer particles to immobilize tPA—fundamentally improves thrombolysis. By merely
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In vivo evaluation of decellularized skeletal muscle matrices for skeletal muscle repair: A systematic review Bioeng. Transl. Med. (IF 6.1) Pub Date : 2025-03-12 Ina Hennion, Charlot Philips, Chong Jiang, Nele Van De Winkel, Laurens J. Ceulemans, Lieven Thorrez
Volumetric muscle loss is the significant loss of skeletal muscle volume beyond the innate regenerative capacity, resulting in functional impairment. The current standard of care combines muscle autografting with physical therapy but is often insufficient to reach full recovery. Decellularized skeletal muscle (DSM) provides an interesting alternative to repair volumetric muscle loss. The native structure
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Engineered microvascular basement membrane mimetic for real‐time neutrophil tracking in the microvascular wall Bioeng. Transl. Med. (IF 6.1) Pub Date : 2025-03-12 Laura C. Morales, Catherine D. Kim, Yangang Pan, Simon Scheuring, Anjelica L. Gonzalez
The microvascular basement membrane (mvBM) is crucial in maintaining vascular integrity and function and, therefore, key to the health of major organs. However, the complex nature and the intricate interplay of biochemical and biomechanical factors that regulate the mvBM functional dynamics make it difficult to study. Here, we present a novel and highly tunable in vitro model of the human mvBM, enabling
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Synergistic drug combination screening using a nanodroplet processing platform to enhance neuroblastoma treatment in TH‐MYCN transgenic mice Bioeng. Transl. Med. (IF 6.1) Pub Date : 2025-03-12 Yen‐Tzu Liao, Zhi‐Kai Yu, Yi‐Xun Huang, Kuan‐Hung Lin, Ching‐Te Kuo, Tsai‐Shan Yang, Pei‐Yi Wu, Chi‐Tai Yeh, Yen‐Lin Liu, Chien‐Chin Chen, Chiung‐Nien Chen, Wen‐Ming Hsu, Hsinyu Lee
Neuroblastoma is a highly aggressive pediatric cancer with a poor prognosis, particularly in high‐risk (HR) cases characterized by MYCN amplification. The severe side effects associated with high‐dose chemotherapy further complicate treatment. Despite significant advancements in drug screening, traditional platforms remain limited due to their requirement for large cell quantities and their low translational
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Bisulfite‐free PCDHGB7 methylation in urine enables early noninvasive detection of urothelial carcinoma Bioeng. Transl. Med. (IF 6.1) Pub Date : 2025-03-11 Zhicong Yang, Qing Chen, Shihua Dong, Peng Xu, Zhanrui Mao, Yaping Dong, Wei Li, Wenxuan Li, Yang Han, Lihe Dai, Gehong Dong, Yong Zhang, Yinshan Li, Liang Cheng, Weimin Ci, Wenqiang Yu, Chuanliang Xu
Urothelial carcinomas (UCs) are the fourth most common male malignancies. However, currently implemented detection methods for UC are usually invasive and/or show passable sensitivity and specificity. An accurate, practical, and effective approach is urgently needed for UC clinical detection. Based on the observation that PCDHGB7 was hypermethylated in UC, we developed a bisulfite‐free quantitative
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Integrated ATAC‐seq and RNA‐seq analysis identifies key regulatory elements in NK cells activated with feeder cells and IL‐2 Bioeng. Transl. Med. (IF 6.1) Pub Date : 2025-03-05 Pedram Motallebnejad, Zion Lee, Jennifer L. One, Frank Cichocki, Wei‐Shou Hu, Samira M. Azarin
Natural killer (NK) cells are in development for allogeneic immunotherapy; however, for such use as off‐the‐shelf medicines, NK cells need to undergo ex vivo expansion, typically through activation with feeder cells and cytokines, to generate sufficient cells for clinical applications. Upon stimulation with feeder cells in the presence of cytokines, NK cells undergo profound changes in gene expression
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Capture of Group A Streptococcus by open‐microfluidic CandyCollect device in pediatric patients Bioeng. Transl. Med. (IF 6.1) Pub Date : 2025-03-04 Wan‐chen Tu, Ingrid H. Robertson, Andrea Blom, Elena Alfaro, Victoria A. M. Shinkawa, Daniel B. Hatchett, Juan C. Sanchez, Anika M. McManamen, Xiaojing Su, Erwin Berthier, Sanitta Thongpang, Ellen R. Wald, Gregory P. DeMuri, Ashleigh B. Theberge
State the PurposeObtaining high‐quality samples to diagnose streptococcal pharyngitis in pediatric patients is challenging due to discomfort associated with traditional pharyngeal swabs. This may cause reluctance to go to the clinic, inaccurate diagnosis, or inappropriate treatment for children with sore throats. Here, we determined the efficacy of CandyCollect, a lollipop‐inspired open‐microfluidic
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Biomembrane‐coated nanosystems as next‐generation delivery systems for the treatment of gastrointestinal cancers Bioeng. Transl. Med. (IF 6.1) Pub Date : 2025-03-02 Joana Lopes, Daniela Lopes, Mahzad Motallebi, Mengguang Ye, Yuxiang Xue, Amélia C. F. Vieira, Sachin Kumar Singh, Kamal Dua, Francisco Veiga, Gautam Sethi, Ana Cláudia Paiva‐Santos, Pooyan Makvandi
Gastrointestinal cancers, a major global cause of cancer‐related mortality and disease burden, are a heterogeneous group of malignant aliments involving different organs of the digestive system. The late clinical diagnosis, genomic tumor heterogeneity, high complexity of the gastrointestinal tumor microenvironment, along with increasing treatment resistance have been recognized as the main contributing
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Noninvasive monitoring of vascular alterations in mice with acute lower limb ischemia using multimodal photoacoustic imaging Bioeng. Transl. Med. (IF 6.1) Pub Date : 2025-02-18 Heng Wang, Keyi Fan, Yijie Ning, Chuanlong Lu, Yuhang Zhang, Qian Wang, Hongjiu Zhang, Yaling Li, Zeyu Zhang, Xiaohua Jia, Sheng Yan, Ruijing Zhang, Honglin Dong
Acute limb ischemia (ALI), especially acute lower limb ischemia (ALLI), is a common clinical vascular emergency with high amputation and mortality rates. However, major challenges exist in rapidly diagnosing and assessing collateral vascular compensatory capacity, leading to appropriate clinical treatment strategies to improve limb preservation and reduce recurrence rates. Traditional imaging methods
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Carboxymethyl cellulose‐polylactic acid particles for inhibiting anoikis and enhancing wound healing efficacy of human mesenchymal stem cells Bioeng. Transl. Med. (IF 6.1) Pub Date : 2025-02-12 Dong‐Hyun Lee, You Bin Lee, Hyun Su Park, Young‐Ju Jang, Youn Chul Kim, Suk Ho Bhang
Adult human mesenchymal stem cells (hMSCs) injection into the wound site promotes angiogenesis and the wound‐closing process by secreting various growth and immune‐modulating factors. However, lower cell attachment sites and the hypoxic microenvironment in the wound site limit their viability and engraftment rate, leading to programmed cell death, anoikis. We synthesized carboxymethyl cellulose‐coated
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Stem cell therapies in the clinic Bioeng. Transl. Med. (IF 6.1) Pub Date : 2025-02-04 Shrinivas Acharya, Suyog Shaha, Michael Griffith Bibbey, Malini Mukherji, Zongmin Zhao, Samir Mitragotri
Stem cell therapies have emerged as a transformative approach in modern medicine, with the potential to address and possibly cure a broad spectrum of diseases. These therapies utilize living stem cells that can perform complex biological functions not replicable by traditional drugs. Stem cell therapies have an expanding therapeutic landscape, with several products already approved and numerous clinical
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Multimodal near‐infrared molecular imaging of ex vivo endometrial carcinoma via CD47‐based targeted tracer Bioeng. Transl. Med. (IF 6.1) Pub Date : 2025-02-04 Jing Lei, Dianfeng Tian, Bo Zhang, Hongrui Guo, Huancheng Su, Jinzheng Wei, Shuai Li, Sufen Li, Chao Liu, Xiaofeng Yang, Sanyuan Zhang
The detection and complete eradication of early‐stage small tumors during hysteroscopy remains a significant clinical challenge in preserving fertility for young women with endometrial cancer (EC). The purpose of this study is to verify the feasibility of CD47 as an optical molecular imaging (OMI) target for human EC and to achieve precise localization and identification in hysteroscopic surgery. The
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Machine learning‐assisted point‐of‐care diagnostics for cardiovascular healthcare Bioeng. Transl. Med. (IF 6.1) Pub Date : 2025-02-04 Kaidong Wang, Bing Tan, Xinfei Wang, Shicheng Qiu, Qiuping Zhang, Shaolei Wang, Ying‐Tzu Yen, Nan Jing, Changming Liu, Xuxu Chen, Shichang Liu, Yan Yu
Cardiovascular diseases (CVDs) continue to drive global mortality rates, underscoring an urgent need for advancements in healthcare solutions. The development of point‐of‐care (POC) devices that provide rapid diagnostic services near patients has garnered substantial attention, especially as traditional healthcare systems face challenges such as delayed diagnoses, inadequate care, and rising medical
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AI‐assisted warfarin dose optimisation with CURATE.AI for clinical impact: Retrospective data analysis Bioeng. Transl. Med. (IF 6.1) Pub Date : 2025-02-04 Tiffany Rui Xuan Gan, Lester W. J. Tan, Mathias Egermark, Anh T. L. Truong, Kirthika Kumar, Shi‐Bei Tan, Sarah Tang, Agata Blasiak, Boon Cher Goh, Kee Yuan Ngiam, Dean Ho
BackgroundStandard‐of‐care for warfarin dose titration is conventionally based on physician‐guided drug dosing. This may lead to frequent deviations from target international normalized ratio (INR) due to inter‐ and intra‐patient variability and may potentially result in adverse events including recurrent thromboembolism and life‐threatening hemorrhage.ObjectivesWe aim to employ CURATE.AI, a small‐data
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Establishing a scalable perfusion strategy for the manufacture of CAR‐T cells in stirred‐tank bioreactors using a quality‐by‐design approach Bioeng. Transl. Med. (IF 6.1) Pub Date : 2025-01-29 Tiffany Hood, Pierre Springuel, Fern Slingsby, Viktor Sandner, Winfried Geis, Timo Schmidberger, Nicola Bevan, Quentin Vicard, Julia Hengst, Noushin Dianat, Qasim A. Rafiq
Chimeric antigen receptor T cell (CAR‐T) therapies show high remission rates for relapsed and refractory leukemia and lymphoma. However, manufacturing challenges hinder their commercial viability and patient accessibility. This study applied quality‐by‐design principles to identify perfusion critical process parameters for CAR‐T expansion in stirred tank bioreactors to maximize yields. A design of
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A non‐surgical method for subretinal delivery by trans‐scleral microneedle injection Bioeng. Transl. Med. (IF 6.1) Pub Date : 2025-01-28 Amir Hejri, Micah A. Chrenek, Nolan T. Goehring, Isabella I. Bowland, Richard Noel, Jiong Yan, John M. Nickerson, Mark R. Prausnitz
Novel therapeutics have emerged for treating neurodegenerative eye diseases but are limited by non‐optimal methods of ocular administration. Subretinal injection is the preferred method of delivery for retinal gene and stem‐cell therapies, but its invasive and complex surgical procedure is a major limiting factor in clinical investigations and practice. Here, we engineered a novel trans‐scleral injection
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Investigating the impact of synonymous gene recoding on a recombinantly expressed monoclonal antibody under different process parameters Bioeng. Transl. Med. (IF 6.1) Pub Date : 2025-01-28 Nayiri M. Kaissarian, Stephanie L. Sandefur, Arnab Ghosh, Upendra K. Katneni, Wendy Walton, Christopher C. Frye, Anton A. Komar, Chava Kimchi‐Sarfaty
Monoclonal antibodies (mAbs) are commonly used biologic therapeutics with a wide variety of clinical applications. During the development process, manufacturers consider different production parameters to improve protein yield and achieve appropriate quality of the product. Synonymous gene recoding is one of such attributes that is often considered and implemented to enhance protein expression. However
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Amelioration of biased neuronal differentiation in humanized mouse model of valproic acid‐induced autism by precisely targeted transcranial magnetic stimulation Bioeng. Transl. Med. (IF 6.1) Pub Date : 2025-01-24 Yilin Hou, Youyi Zhao, Dingding Yang, Tingwei Feng, Yuqian Li, Xiang Li, Zhou'an Liu, Xiao Yan, Hui Zhang, Shengxi Wu, Xufeng Liu, Yazhou Wang
Autism spectrum disorder (ASD) is a group of developmental diseases, which still lacks effective treatments. Pregnant exposure of Valproic acid (VPA) is an important environmental risk factor for ASD, but it's long‐term effects on the development of human neural cells, particularly in vivo, and the corresponding treatment have yet been fully investigated. In the present study, we first made a humanized
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Milk extracellular vesicles: A burgeoning new presence in nutraceuticals and drug delivery Bioeng. Transl. Med. (IF 6.1) Pub Date : 2025-01-23 Spencer R. Marsh, Claire E. Beard, Robert G. Gourdie
Mammalian milk, a multifaceted developmental biofluid, has attracted new attention due to its diverse constituents and their implications for health and disease. Among these constituents, extracellular vesicles (EVs) have emerged as focal points of investigation. EVs, including exosomes and small EVs, have demonstrated biological activity in preclinical studies—including reports of enhancement of cognition
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Establishment of a chemoresistant laryngeal cancer cell model to study chemoresistance and chemosensitization responses via transcriptomic analysis and a tumor‐on‐a‐chip platform Bioeng. Transl. Med. (IF 6.1) Pub Date : 2025-01-22 Christian R. Moya‐Garcia, Meghana Munipalle, Alain Pacis, Nader Sadeghi, Maryam Tabrizian, Nicole Y. K. Li‐Jessen
Tumor resistance to chemotherapy is a common cause of cancer recurrence in patients with head and neck squamous cell carcinoma. The goal of this study is to establish and characterize a chemoresistant laryngeal cancer cell model and test its potential utility for chemosensitizing therapy. At the genotypic level, RNA sequencing confirmed that the cells acquired putative resistance with upregulated docetaxel‐resistant
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Microbead‐based synthetic niches for in vitro expansion and differentiation of human naïve B‐cells Bioeng. Transl. Med. (IF 6.1) Pub Date : 2025-01-17 Pearlson Prashanth Austin Suthanthiraraj, Sydney Bone, Kyung‐Ho Roh
As the prospect of engineering primary B‐cells for cellular therapies in cancer, autoimmune diseases, and infectious diseases grows, there is an increasing demand for robust in vitro culture systems that effectively activate human B‐cells isolated from peripheral blood for consistent and efficient expansion and differentiation into various effector phenotypes. Feeder cell‐based systems have shown promise
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The BAM‐GelMA‐ADSCs bilayer patch promotes tissue regeneration and functional recovery after large‐area bladder defects in beagles Bioeng. Transl. Med. (IF 6.1) Pub Date : 2025-01-15 Ziyan An, Pengchao Wang, Zhengyun Ling, Kaipeng Bi, Zheng Wang, Jinpeng Shao, Jian Zhao, Zhouyang Fu, Meng Huang, Wenjie Wei, Shuwei Xiao, Jin Zhou, Weijun Fu
Previous studies of bladder tissue engineering simply seeded cells onto the surface of the material, which makes the cells lack protection and makes it difficult to face the complex in vivo environment. The gelatin methacryloyl (GelMA) hydrogel possesses outstanding biocompatibility and distinctive photo‐crosslinking characteristics and is capable of offering a suitable three‐dimensional growth environment
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Convective forces contribute to post‐traumatic degeneration after spinal cord injury Bioeng. Transl. Med. (IF 6.1) Pub Date : 2025-01-14 Hoi Y. Kwon, Christopher Streilein, R. Chase Cornelison
Spinal cord injury (SCI) initiates a complex cascade of chemical and biophysical phenomena that result in tissue swelling, progressive neural degeneration, and formation of a fluid‐filled cavity. Previous studies show fluid pressure above the spinal cord (supraspinal) is elevated for at least 3 days after injury and contributes to a phase of damage called secondary injury. Currently, it is unknown
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Artificial intelligence‐enabled innovations in cochlear implant technology: Advancing auditory prosthetics for hearing restoration Bioeng. Transl. Med. (IF 6.1) Pub Date : 2025-01-10 Guodao Zhang, Rui Chen, Hamzeh Ghorbani, Wanqing Li, Arsen Minasyan, Yideng Huang, Sen Lin, Minmin Shao
This comprehensive review explores the implications of artificial intelligence (AI) in addressing cochlear implant (CI) issues and revolutionizing the landscape of auditory prosthetics. It begins with an overview of ear anatomy and hearing loss, then explores a review of CI technology and its current challenges. The review emphasizes how advanced AI algorithms and data‐driven approaches enhance CI
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Mesenchymal stem cell extracellular vesicle vascularization bioactivity and production yield are responsive to cell culture substrate stiffness Bioeng. Transl. Med. (IF 6.1) Pub Date : 2025-01-07 Emily H. Powsner, Stephanie M. Kronstadt, Kristin Nikolov, Amaya Aranda, Steven M. Jay
Mesenchymal stem cell‐derived extracellular vesicles (MSC EVs) are an attractive therapeutic option for regenerative medicine applications due to their inherently pro‐angiogenic and anti‐inflammatory properties. However, reproducible and cost‐effective production of highly potent therapeutic MSC EVs is challenging, limiting their translational potential. Here, we investigated whether the well‐characterized
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Ultrasound‐guided interstitial photothermal therapy generates improved treatment responses in a 9464D model of neuroblastoma Bioeng. Transl. Med. (IF 6.1) Pub Date : 2025-01-06 Grace E. Olsson, Rohan V. Patil, Samantha J. Chin, Katharine N. Rus, Elizabeth E. Sweeney, Karun V. Sharma, Rohan Fernandes
We describe the use of ultrasound image guidance to improve treatment outcomes when administering interstitial photothermal therapy (I‐PTT), an experimental cancer treatment modality. I‐PTT is a promising thermal therapy for tumors using intratumorally injected nanoparticle‐based photothermal agents activated by an interstitially placed laser diffuser. We hypothesized that ultrasound‐based image guidance
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Mpox disease, diagnosis, and point of care platforms Bioeng. Transl. Med. (IF 6.1) Pub Date : 2025-01-03 Nazente Atceken, Ikra Bayaki, Berk Can, Defne Yigci, Savas Tasoglu
Human Mpox disease (MPX) is an endemic zoonotic disease that develops when patients are infected with the Mpox virus (MPXV). MPXV shares a high level of genetic similarity to other poxviruses and the clinical presentation of MPX is similar to other poxvirus infections which can result in a delay in diagnosis. In addition, the MPXV virus is phylogenetically divided into two different clades which affects
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Ultrasound‐mediated mechanical forces activate selective tumor cell apoptosis Bioeng. Transl. Med. (IF 6.1) Pub Date : 2024-12-31 Ajay Tijore, Felix Margadant, Nehal Dwivedi, Leslie Morgan, Mingxi Yao, Anushya Hariharan, Claire Alexandra Zhen Chew, Simon Powell, Glenn Kunnath Bonney, Michael Sheetz
Recent studies show that tumor cells undergo apoptosis after mechanical stretching, which promotes normal cell growth. Since ultrasound can produce similar sub‐cellular mechanical stresses on the nanoscale, here we test the effect of ultrasound‐mediated mechanical forces on tumors and normal cell survival. Surprisingly, tumor cells undergo apoptosis through a calpain‐dependent mitochondrial pathway
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Catalpol promotes the generation of cerebral organoids with oRGs through activation of STAT3 signaling Bioeng. Transl. Med. (IF 6.1) Pub Date : 2024-12-30 Yoo‐Jung Lee, Byounggook Cho, Daeyeol Kwon, Yunkyung Kim, Saemin An, Soi Kang, Jongpil Kim
The generation of human cortical organoids containing outer radial glia (oRG) cells is crucial for modeling neocortical development. Here we show that Catalpol, an iridoid glucoside derived from Rehmannia glutinosa, significantly enhances the generation of cerebral organoids with expanded oRG populations and increased neurogenic potential. Catalpol‐treated organoids exhibited thicker ventricular zone/subventricular
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Recent developments in microwire‐structured intracortical electrode arrays for brain–machine interfaces Bioeng. Transl. Med. (IF 6.1) Pub Date : 2024-12-20 Sorel E. De León, Simon Higham, Young Jun Jung, Wei Tong, David J. Garrett
Brain–machine interfaces (BMIs) have experienced remarkable advancements in recent years, marked by multiple companies initiating human trials. Consequently, the interface between the brain and electrodes has become more critical than ever, requiring implanted electrodes to be not only biocompatible and minimally invasive but also capable of remaining functioning in the brain for a lifetime. While
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Wearable photobiomodulation halts thyroid cancer growth by leveraging thyroid photosensitivity Bioeng. Transl. Med. (IF 6.1) Pub Date : 2024-12-20 Changrui Zhao, Kun Fu, Jiameng Tian, Tian Long, Jianzhong Song, Siyu Chen, Chang Liu
With papillary thyroid carcinoma (PTC) rates rising significantly, concerns about conventional treatments like thyroidectomy and radiotherapy highlight the need for non‐invasive options. Our study explores photobiomodulation therapy (PBMT), which uses specific light wavelengths to evoke cellular responses in PTC treatment. Our research utilized a custom‐designed optical system to investigate PBMT,
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The potential of low‐intensity pulsed ultrasound to apply the long‐term ovary protection from injury induced by 4‐vinylcyclohexene diepoxide through inhibiting granulosa cell apoptosis Bioeng. Transl. Med. (IF 6.1) Pub Date : 2024-12-17 Juan Deng, Juan Qin, Guolin Song, Chenghai Li, Wentao Tang, Yilin Tang, Xinfang Xiao, Liu Wu, Sicheng He, Yiqing Zhou, Junfen Li, Yan Wang
The potential of low‐intensity pulsed ultrasound (LIPUS) in regulating ovarian function has been demonstrated; however, there is a lack of scientific evidence regarding the long‐term efficacy of LIPUS in treating ovarian injury and understanding its regulatory mechanisms. In this study, 4‐vinylcyclohexene diepoxide (VCD) was used to induce ovarian injury in rats, and LIPUS was applied to target the
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Nanoparticle contrast‐enhanced computed tomography and magnetic resonance imaging of vascularization of a subcutaneous niche for islet transplantation Bioeng. Transl. Med. (IF 6.1) Pub Date : 2024-12-14 Simone Capuani, Jocelyn Nikita Campa‐Carranza, Nathanael Hernandez, Renuka T. R. Menon, Rohan Bhavane, Gabrielle E. Rome, Laxman Devkota, Ketan B. Ghaghada, Ananth V. Annapragada, Corrine Ying Xuan Chua, Andrew A. Badachhape, Alessandro Grattoni
Revascularization plays a critical role in the successful engraftment of transplanted pancreatic islets, which are inherently rich in capillaries to meet their high metabolic demands. Innovative islet encapsulation strategies such as the NICHE (neovascularized implantable cell homing and encapsulation), generate a prevascularized transplantation site that allows for direct integration of the graft
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Assessing the impact of airborne particulate pollution on human skin utilizing a novel human skin equivalent containing MUTZ‐3‐derived Langerhans cells Bioeng. Transl. Med. (IF 6.1) Pub Date : 2024-12-14 Amy Simpson, Teresa DiColandrea, Stefan Przyborski
Air pollution is an exogenous stressor known to have a detrimental impact on skin health through the induction of inflammation; however, the direct effect of topical pollution exposure is still being elucidated. Human skin equivalents (HSE) aim to reproduce in vitro the structure and function of the native skin tissue. However, HSEs typically lack skin‐resident immune cells, which could play a key
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Reduced glutathione enhances adipose tissue‐derived mesenchymal stem cell engraftment efficiency for liver fibrosis by targeting TGFβ1/SMAD3/NOX4 pathway Bioeng. Transl. Med. (IF 6.1) Pub Date : 2024-12-10 Shaoxiong Yu, Yingchao Wang, Yingjun Shi, Saihua Yu, Bixing Zhao, Naishun Liao, Xiaolong Liu
Reduced glutathione (GSH) could reduce oxidative stress to improve adipose tissue‐derived mesenchymal stem cell (ADSC) engraftment efficiency in vivo. However, the underlying mechanisms remain unclear. Our goal is to investigate whether GSH enhances ADSC engraftment through targeting the TGFβ/SMAD3/NOX4 pathway. Liver fibrotic male mice were administrated GSH, setanaxib (STX), and SIS3 during ADSC
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Green/red fluorescent protein disrupting drugs for real‐time permeability tracking in three‐dimensional tumor spheroids Bioeng. Transl. Med. (IF 6.1) Pub Date : 2024-12-09 Maytal Avrashami, Danna Niezni, Dana Meron Azagury, Hagit Sason, Yosi Shamay
Three‐dimensional (3D) spheroid models offer a more physiologically relevant and complex environment compared to traditional two‐dimensional cultures, making them a promising tool for studying tumor biology and drug response. However, these models often face challenges in real‐time monitoring of drug diffusion, penetration, and target engagement, limiting their predictive power for in vivo and clinical