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Personalized additive manufacturing of devices for the management of enteroatmospheric fistulas Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-09-26 Francisco José Calero Castro, Andrés Padillo Eguía, Virginia Durán Muñoz-Cruzado, Luis Tallón Aguilar, José Tinoco González, Imán Laga, Fernando de la Portilla de Juan, Felipe Pareja Ciuró, Javier Padillo Ruiz
Additive manufacturing techniques allow the customized design of medical devices according to the patient's requirements. Enteroatmospheric fistula is a pathology that benefits from this personalization due to its extensive clinical variability since the size and morphology of the wound differ extensively among patients. Standard prosthetics do not achieve proper isolation of the wound, leading to
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Rapid, label-free enrichment of lymphocytes in a closed system using a flow-through microfluidic device Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-09-25 Anton Mukhamedshin, Riley C. Reddington, Mai T. P. Dinh, Kumar Abhishek, Mubasher Iqbal, Marc Manheim, Sean C. Gifford, Sergey S. Shevkoplyas
The majority of adoptive cellular therapies are produced from peripheral mononuclear cells obtained via leukapheresis and further enriched for the cells of interest (e.g., T cells). Here, we present a first-of-its-kind closed system, which effectively removes ~85% of monocytes and ~88% of platelets, while recovering ~88% of concentrated T cells in a separate output stream, as the leukapheresis sample
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PEGylated therapeutics in the clinic Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-09-22 Yongsheng Gao, Maithili Joshi, Zongmin Zhao, Samir Mitragotri
The covalent attachment of polyethylene glycol (PEG) to therapeutic agents, termed PEGylation, is a well-established and clinically proven drug delivery approach to improve the pharmacokinetics and pharmacodynamics of drugs. Specifically, PEGylation can improve the parent drug's solubility, extend its circulation time, and reduce its immunogenicity, with minimal undesirable properties. PEGylation technology
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Tissue adhesive indocyanine green-locking granular gel-mediated photothermal therapy combined with checkpoint inhibitor for preventing postsurgical recurrence and metastasis of colorectal cancer Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-09-18 Yuan Zeting, Ma Shuli, Li Yue, Fang Haowei, Shang Jing, Zhan Yueping, Wang Jie, Chen Teng, Deng Wanli, Kunxi Zhang, Yin Peihao
Developing effective therapy to inhibit postoperative recurrence and metastasis of colorectal cancer (CRC) is challenging and significant to reduce mortality and morbidity. Here, a granular hydrogel, assembled from gelatin microgels by dialdehyde starch and interpenetrated with in situ polymerized poly(sulfobetaine methacrylate-co-N-isopropylacrylamide) (P(SBMA-co-NIPAM)), is prepared to load and lock
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Intratumoral nanofluidic system enhanced tumor biodistribution of PD-L1 antibody in triple-negative breast cancer Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-09-15 Hsuan-Chen Liu, Simone Capuani, Andrew A. Badachhape, Nicola Di Trani, Daniel Davila Gonzalez, Robin S. Vander Pol, Dixita I. Viswanath, Shani Saunders, Nathanael Hernandez, Ketan B. Ghaghada, Shu-Hsia Chen, Elizabeth Nance, Ananth V. Annapragada, Corrine Ying Xuan Chua, Alessandro Grattoni
Immune checkpoint inhibitors (ICI), pembrolizumab and atezolizumab, were recently approved for treatment-refractory triple-negative breast cancer (TNBC), where those with Programmed death-ligand 1 (PD-L1) positive early-stage disease had improved responses. ICIs are administered systemically in the clinic, however, reaching effective therapeutic dosing is challenging due to severe off-tumor toxicities
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Using biomaterials to improve mesenchymal stem cell therapies for chronic, nonhealing wounds Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-09-13 Romina Keshavarz, Sara Olsen, Bethany Almeida
Historically, treatment of chronic, nonhealing wounds has focused on managing symptoms using biomaterial-based wound dressings, which do not adequately address the underlying clinical issue. Mesenchymal stem cells (MSCs) are a promising cell-based therapy for the treatment of chronic, nonhealing wounds, yet inherent cellular heterogeneity and susceptibility to death during injection limit their clinical
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Sound conditioning strategy promoting paracellular permeability of the blood-labyrinth-barrier benefits inner ear drug delivery Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-09-10 Xueling Wang, Jiayi Gu, Ke Xu, Baoying Xu, Dehong Yu, Hao Wu
The therapeutic effects of pharmaceuticals depend on their drug concentrations in the cochlea. Efficient drug delivery from the systemic circulation into the inner ear is limited by the blood-labyrinth-barrier (BLB). This study investigated a novel noninvasive sound conditioning (SC) strategy (90 dB SPL, 8–16 kHz, 2 h sound exposure) to temporally enhance BLB permeability in a controllable way, contributing
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Novel metal peroxide nanoboxes restrain Clostridioides difficile infection beyond the bactericidal and sporicidal activity Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-09-05 Li-Xing Yang, Yi-Hsin Lai, Chun In Cheung, Zhi Ye, Tzu-Chi Huang, Yu-Chin Wang, Yu-Cheng Chin, Zi-Chun Chia, Ya-Jyun Chen, Meng-Jia Li, Hsiu-Ying Tseng, Yi-Tseng Tsai, Zhi-Bin Zhang, Kuan-Hsu Chen, Bo-Yang Tsai, Dar-Bin Shieh, Nan-Yao Lee, Pei-Jane Tsai, Chih-Chia Huang
Clostridioides difficile spores are considered as the major source responsible for the development of C. difficile infection (CDI), which is associated with an increased risk of death in patients and has become an important issue in infection control of nosocomial infections. Current treatment against CDI still relies on antibiotics, which also damage normal flora and increase the risk of CDI recurrence
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Distinguished biomimetic dECM system facilitates early detection of metastatic breast cancer cells Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-09-01 Bowen Weng, Mei Li, Weilai Zhu, Jing Peng, Xufeng Mao, Yanan Zheng, Chi Zhang, Senhao Pan, Haijiao Mao, Jiyuan Zhao
Breast cancer is the most prevalent malignant tumor affecting women's health. Bone is the most common distant metastatic organ, worsening the quality of life and increasing the mortality of patients. Early detection of breast cancer bone metastasis is urgent for halting disease progression and improving tumor prognosis. Recently, extracellular matrix (ECM) with biomimetic tissue niches opened a new
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Predicting the onset of preeclampsia by longitudinal monitoring of metabolic changes throughout pregnancy with Raman spectroscopy Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-08-31 Saman Ghazvini, Saji Uthaman, Lilly Synan, Eugene C. Lin, Soumik Sarkar, Mark K. Santillan, Donna A. Santillan, Rizia Bardhan
Preeclampsia is a life-threatening pregnancy disorder. Current clinical assays cannot predict the onset of preeclampsia until the late 2nd trimester, which often leads to poor maternal and neonatal outcomes. Here we show that Raman spectroscopy combined with machine learning in pregnant patient plasma enables rapid, highly sensitive maternal metabolome screening that predicts preeclampsia as early
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Modeling diabetic endothelial dysfunction with patient-specific induced pluripotent stem cells Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-08-30 Rayyan Gorashi, Nancy Rivera-Bolanos, Caitlyn Dang, Cedric Chai, Beatrix Kovacs, Sara Alharbi, Syeda Subia Ahmed, Yogesh Goyal, Guillermo Ameer, Bin Jiang
Diabetes is a known risk factor for various cardiovascular complications, mediated by endothelial dysfunction. Despite the high prevalence of this metabolic disorder, there is a lack of in vitro models that recapitulate the complexity of genetic and environmental factors associated with diabetic endothelial dysfunction. Here, we utilized human induced pluripotent stem cell (iPSC)-derived endothelial
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4D-CT deformable image registration using unsupervised recursive cascaded full-resolution residual networks Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-08-22 Lei Xu, Ping Jiang, Tiffany Tsui, Junyan Liu, Xiping Zhang, Lequan Yu, Tianye Niu
A novel recursive cascaded full-resolution residual network (RCFRR-Net) for abdominal four-dimensional computed tomography (4D-CT) image registration was proposed. The entire network was end-to-end and trained in the unsupervised approach, which meant that the deformation vector field, which presented the ground truth, was not needed during training. The network was designed by cascading three full-resolution
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Optimization of ionizable lipids for aerosolizable mRNA lipid nanoparticles Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-08-21 Mae M. Lewis, Melissa R. Soto, Esther Y. Maier, Steven D. Wulfe, Sandy Bakheet, Hannah Obregon, Debadyuti Ghosh
Although mRNA lipid nanoparticles (LNPs) are highly effective as vaccines, their efficacy for pulmonary delivery has not yet fully been established. A major barrier to this therapeutic goal is their instability during aerosolization for local delivery. This imparts a shear force that degrades the mRNA cargo and therefore reduces cell transfection. In addition to remaining stable upon aerosolization
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Biodegradable scaffolds for enhancing vaccine delivery Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-08-21 Matthew D. Kerr, Wade T. Johnson, David A. McBride, Arun K. Chumber, Nisarg J. Shah
Sustained release of vaccine components is a potential method to boost efficacy compared with traditional bolus injection. Here, we show that a biodegradable hyaluronic acid (HA)-scaffold, termed HA cryogel, mediates sustained antigen and adjuvant release in vivo leading to a durable immune response. Delivery from subcutaneously injected HA cryogels was assessed and a formulation which enhanced the
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Role of human dural fibroblasts in the angiogenic responses of human endothelial cells: An in vitro dural model and the application of lab-on-a-chip for EDAS Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-08-19 Woo-Keun Kwon, Chang-Min Yoo, Jang Hun Kim, Tae-Won Kim, An-Gi Kim, Min-Ho Hwang, Hyuk Choi
Encephaloduroarteriosynangiosis (EDAS), an indirect anastomosis procedure, is widely accepted as a primary treatment for moyamoya disease (MMD) to improve collateral blood flow. During surgical intervention, dural fibroblasts (DuF) are thought to produce various proteins that create an angiogenic microenvironment. However, the biophysiological evidence supporting the angiogenic properties of this surgical
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Remote limb ischemic postconditioning inhibits microglia pyroptosis by modulating HGF after acute ischemia stroke Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-08-19 Lu Yu, Zuohui Zhang, Hao Chen, Miao Wang, Wenqi Mao, Jinxia Hu, Dandan Zuo, Bingchen Lv, Weifeng Wu, Suhua Qi, Guiyun Cui
The repetitive inflation–deflation of a blood pressure cuff on a limb is known as remote limb ischemic postconditioning (RIPostC). It prevents brain damage induced by acute ischemia stroke (AIS). Pyroptosis, executed by the pore-forming protein gasdermin D (GSDMD), is a type of regulated cell death triggered by proinflammatory signals. It contributes to the pathogenesis of ischemic brain injury. However
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Use of patient-derived tumor organoid platform to predict the benefit of postoperative adjuvant chemotherapy for poor responders to neoadjuvant chemoradiotherapy in locally advanced rectal cancer Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-08-16 Weisong Xue, Ting Wang, Jiaxin Yao, Wei Wu, Dexin Chen, Botao Yan, Xiaoyu Dong, Yuting Tang, Yunli Zeng, Yueyu He, Peihua Cao, Fangyuan Shao, Wenhua Huang, Chuxia Deng, Jun Yan
Postoperative adjuvant chemotherapy (AC) for poor responders to neoadjuvant chemoradiotherapy (nCRT) remains debatable among patients with locally advanced rectal cancer (LARC), necessitating biomarkers to accurately predict the benefits of AC. This study aimed to develop a patient-derived tumor organoid (PDTO) platform to predict the benefit of AC in LARC patients showing poor nCRT response. PDTOs
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The emerging role of heart-on-a-chip systems in delineating mechanisms of SARS-CoV-2-induced cardiac dysfunction Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-08-08 Rick Xing Ze Lu, Yimu Zhao, Milica Radisic
Coronavirus disease 2019 (COVID-19) has been a major global health concern since its emergence in 2019, with over 680 million confirmed cases as of April 2023. While COVID-19 has been strongly associated with the development of cardiovascular complications, the specific mechanisms by which viral infection induces myocardial dysfunction remain largely controversial as studies have shown that the severe
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Membrane fusogenic nanoparticle-based HLA-peptide-addressing universal T cell receptor-engineered T (HAUL TCR-T) cell therapy in solid tumor Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-08-07 Ruihan Xu, Qin Wang, Junmeng Zhu, Yuncheng Bei, Yanhong Chu, Zhichen Sun, Shiyao Du, Shujuan Zhou, Naiqing Ding, Fanyan Meng, Baorui Liu
T cell receptor-engineered T (TCR-T) cell therapy has demonstrated therapeutic effects in basic research and clinical trials for treating solid tumors. Due to the peptide-dependent recognition and the human leukocyte antigen (HLA)-restriction, TCR-T cell therapy is generally custom designed to target individual antigens. The lack of suitable universal targets for tumor cells significantly limits its
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A soft, bioinspired artificial lymphatic system for interactive ascites transfer Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-08-03 Peng Wang, Ji Fu, Peng Jin, Jin Zeng, Xiaohui Miao, Heling Wang, Yinji Ma, Xue Feng
Low-flow removal of refractory ascites is critical to treating cirrhosis and digestive system tumor, and thus, commercial ascites pump emerged lately. The rigid structure of clinically available pumps rises complication rate and lack of flow rate monitoring hinders early warning of abnormalities. Herein, a soft artificial system was proposed inspired by lymph for interactive ascites transfer with great
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Prospective therapeutics for intestinal and hepatic fibrosis Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-08-02 Xin Li, Mengli Yu, Qingwei Zhao, Yang Yu
Currently, there are no effective therapies for intestinal and hepatic fibrosis representing a considerable unmet need. Breakthroughs in pathogenesis have accelerated the development of anti-fibrotic therapeutics in recent years. Particularly, with the development of nanotechnology, the harsh environment of the gastrointestinal tract and inaccessible microenvironment of fibrotic lesions seem to be
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A drug-selectable acoustic reporter gene system for human cell ultrasound imaging Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-08-02 Alessandro R. Howells, Phoebe J. Welch, John Kim, Craig R. Forest, Chengzhi Shi, Xiaojun Lance Lian
A promising new field of genetically encoded ultrasound contrast agents in the form of gas vesicles has recently emerged, which could extend the specificity of medical ultrasound imaging. However, given the delicate genetic nature of how these genes are integrated and expressed, current methods of producing gas vesicle-expressing mammalian cell lines requires significant cell processing time to establish
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Bioadhesive microneedle patches for tissue sealing Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-08-01 Eden Freundlich, Neta Shimony, Adi Gross, Boaz Mizrahi
Sealing of soft tissues prevents leakage of gas and liquid, closes wounds, and promotes healing and is, therefore, of great significance in the clinical and medical fields. Although various formulations have been developed for reliable sealing of soft tissue, tradeoffs between adhesive properties, degradation profile, and tissue toxicity limit their clinical use. Hydrogel-based adhesives, for example
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Machine learning chained neural network analysis of oxygen transport amplifies the physiological relevance of vascularized microphysiological systems Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-08-01 James J. Tronolone, Tanmay Mathur, Christopher P. Chaftari, Yuxiang Sun, Abhishek Jain
Since every biological system requires capillaries to support its oxygenation, design of engineered preclinical models of such systems, for example, vascularized microphysiological systems (vMPS) have gained attention enhancing the physiological relevance of human biology and therapies. But the physiology and function of formed vessels in the vMPS is currently assessed by non-standardized, user-dependent
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Electrophysiological mapping of the epicardium via 3D-printed flexible arrays Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-07-19 Terrence Pong, Kevin J. Cyr, Cody Carlton, Joy Aparicio-Valenzuela, Hanjay Wang, Meghedi Babakhanian, Alessandro Maiuolo, Haley Lucian, Paul J. Wang, Y. Joseph Woo, Anson M. Lee
Cardiac electrophysiology mapping and ablation are widely used to treat heart rhythm disorders such as atrial fibrillation (AF) and ventricular tachycardia (VT). Here, we describe an approach for rapid production of three dimensional (3D)-printed mapping devices derived from magnetic resonance imaging. The mapping devices are equipped with flexible electronic arrays that are shaped to match the epicardial
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An engineered interleukin-1 decoy cytokine inhibits receptor signaling and proliferation in lung adenocarcinoma Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-07-18 Brianna J. McIntosh, Griffin G. Hartmann, Sean A. Yamada-Hunter, Phillip Liu, Camille F. Williams, Julien Sage, Jennifer R. Cochran
The cytokine interleukin (IL)-11 has been shown to play a role in promoting fibrosis and cancer, including lung adenocarcinoma, garnering interest as an attractive target for therapeutic intervention. We used combinatorial methods to engineer an IL-11 variant that binds with higher affinity to the IL-11 receptor and stimulates enhanced receptor-mediated cell signaling. Introduction of two additional
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Injection of a PMMA-doped MSC spheroid gel for the treatment of painful osteoporotic vertebral compression fractures Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-07-16 Wan-Kyu Ko, Daye Lee, Seong Jun Kim, Gong Ho Han, Donghyun Lee, Seung Hun Sheen, Seil Sohn
We aimed to develop a biocompatible treatment to overcome the limitations of polymethyl methacrylate (PMMA) vertebroplasty for osteoporotic compression fracture patients. We synthesized an injectable hydrogel containing PMMA. Mesenchymal stem cell (MSC) spheroids were included in the injectable PMMA-doped gel (= PMMA-doped spheroid gel). In vitro, the osteogenic/anti-inflammatory effects of the embedded
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Pluronic F127 “nanoarmor” for stabilization of Cowpea mosaic virus immunotherapy Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-07-14 Matthew D. Shin, Eunkyeong Jung, Miguel A. Moreno-Gonzalez, Oscar A. Ortega-Rivera, Nicole F. Steinmetz
Our lab demonstrated that intratumoral Cowpea mosaic virus (CPMV) is a potent antitumor immunotherapy when used as in situ vaccine. As we pave the way for human clinical translation, formulation chemistry needs to be optimized for long-term storage of the drug candidate. In this work, CPMV was nanoengineered with Pluronic F127 to realize liquid and gel formulations which mitigate structural changes
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Erratum to “Cell loaded hydrogel containing Ag-doped bioactive glass–ceramic nanoparticles as skin substitute: Antibacterial properties, immune response, and scarless cutaneous wound regeneration” Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-07-14
Sharifi, E, Sadati, SA, Yousefiasl, S, Sartorius, R, Zafari, M, Rezakhani, L, Alizadeh, M, Nazarzadeh Zare, E, Omidghaemi, S, Ghanavatinejad, F, Jami, M-S, Salahinejad, E, Samadian, H, Paiva-Santos, AC, De Berardinis, P, Shafiee, A, Tay, FR, Pourmotabed, S, and Makvandi, P. (2022). Cell loaded hydrogel containing Ag-doped bioactive glass–ceramic nanoparticles as skin substitute: antibacterial properties
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COVID severity test (CoST sensor)—An electrochemical immunosensing approach to stratify disease severity Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-07-11 Sasya Madhurantakam, Jayanth Babu Karnam, Sriram Muthukumar, Shalini Prasad
With the evolution of the COVID-19 pandemic, there is now a need for point-of-care devices for the quantification of disease biomarkers toward disease severity assessment. Disease progression has been determined as a multifactor phenomenon and can be treated based on the host immune response within each individual. CoST is an electrochemical immunosensor point-of-care device that can determine disease
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Engineering T cell receptor fusion proteins using nonviral CRISPR/Cas9 genome editing for cancer immunotherapy Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-07-10 Runzhe Shu, Maree Hammett, Vera Evtimov, Aleta Pupovac, Nhu-Y Nguyen, Rasa Islam, Junli Zhuang, Seyeong Lee, Tae-hun Kang, Kyujun Lee, Ian Nisbet, Peter Hudson, Jae Young Lee, Richard Boyd, Alan Trounson
Manufacture of chimeric antigen receptor (CAR)-T cells usually involves the use of viral delivery systems to achieve high transgene expression. However, it can be costly and may result in random integration of the CAR into the genome, creating several disadvantages including variation in transgene expression, functional gene silencing and potential oncogenic transformation. Here, we optimized the method
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Low-intensity pulsed ultrasound alleviates doxorubicin-induced cardiotoxicity via inhibition of S100a8/a9-mediated cardiac recruitment of neutrophils Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-07-07 Hong Zhu, Min He, Yong-Li Wang, Yuanxin Zhang, Jingsong Dong, Bo-Yan Chen, Yu-Lin Li, Lu-Jun Zhou, Lin-Juan Du, Yuan Liu, Wu-Chang Zhang, Dean Ta, Sheng-Zhong Duan
Doxorubicin (DOX)-induced cardiotoxicity limits its broad use as a chemotherapy agent. The development of effective and non-invasive strategies to prevent DOX-associated adverse cardiac events is urgently needed. We aimed to examine whether and how low-intensity pulsed ultrasound (LIPUS) plays a protective role in DOX-induced cardiotoxicity. Male C57BL/6J mice were used to establish models of both
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Histological assessment of nanostructured fibrin-agarose skin substitutes grafted in burnt patients. A time-course study Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-07-07 Miguel Angel Martin-Piedra, Gloria Carmona, Fernando Campos, Víctor Carriel, Ana Fernández-González, Antonio Campos, Natividad Cuende, Ingrid Garzón, Purificación Gacto, Miguel Alaminos
A previously developed fibrin-agarose skin model—UGRSKIN—showed promising clinical results in severely burnt patients. To determine the histological parameters associated to the biocompatibility and therapeutic effects of this model, we carried out a comprehensive structural and ultrastructural study of UGRSKIN grafted in severely burnt patients after 3 months of follow-up. The grafted epidermis was
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Mesenchymal stem cells paracrine proteins from three-dimensional dynamic culture system promoted wound healing in third-degree burn models Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-07-02 Yingwei Wang, Jiaxin Wu, Jiamin Chen, Cheng Lu, Jinchao Liang, Yingyi Shan, Jie Liu, Qi Li, Liang Miao, Mu He, Xiaoying Wang, Jianhua Zhang, Zheng Wu
Recovery of skin function remains a significant clinical challenge for deep burns owing to the severe scar formation and poor appendage regeneration, and stem cell therapy has shown great potential for injured tissue regeneration. Here, a cell-free therapy system for deep burn skin was explored using mesenchymal stem cell paracrine proteins (MSC-PP) and polyethylene glycol (PEG) temperature-sensitive
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Ultrasound-targeted microbubble destruction mediates PDE5i/NO integration for cavernosum remodeling and penile rehabilitation Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-07-02 Wende Yang, Chen Qiu, Jiancheng Zhai, Wei Zhang, Chengwu Huang, Jun Shao, Jingke Zhang, Shigao Chen, Xiaoyan Miao, Peng Chen, Bo Wei, Jie Ren, Hongbo Wei
Erectile dysfunction (ED) caused by cavernous nerve injury (CNI) is refractory to heal mainly ascribed to the adverse remodeling of the penis induced by ineffectual microvascular perfusion, fibrosis, and neurotrophins scarcity in cavernosum. Phosphodiesterase type V inhibitors (PDE5i) have been regarded as an alternative candidate drug for avoiding penile neuropathy. However, the therapeutic efficacy
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Techniques for navigating postsurgical adhesions: Insights into mechanisms and future directions Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-06-26 Jiahui Chen, Xiaoqi Tang, Ziyu Wang, Arielle Perez, Benjamin Yao, Ke Huang, Yang Zhang, Martin W. King
Postsurgical adhesions are a common complication of surgical procedures that can lead to postoperative pain, bowel obstruction, infertility, as well as complications with future procedures. Several agents have been developed to prevent adhesion formation, such as barriers, anti-inflammatory and fibrinolytic agents. The Food and Drug Administration (FDA) has approved the use of physical barrier agents
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Dual gene-activated dermal scaffolds regulate angiogenesis and wound healing by mediating the coexpression of VEGF and angiopoietin-1 Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-06-25 Tingting Weng, Min Yang, Wei Zhang, Ronghua Jin, Sizhan Xia, Manjia Zhang, Pan Wu, Xiaojie He, Chunmao Han, Xiong Zhao, Xingang Wang
The vascularization of dermal substitutes is a key challenge in efforts to heal deep skin defects. In this study, dual gene-activated dermal scaffolds (DGADSs-1) were fabricated by loading nanocomposite particles of polyethylenimine (PEI)/multiple plasmid DNAs (pDNAs) encoding vascular endothelial growth factor and angiopoietin-1 at a ratio of 1:1. In a similar manner, DGADSs-2 were loaded with a chimeric
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Secure reversal of immune evasion from refractory NSCLC and highly contagious CoV-2 mutants by using 3D-engineered multifunctional biologics Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-06-24 Yanna Zhang, Qian Li, Nanxi Liu, Jianchuan Hu, Xiaojuan Lin, Meijuan Huang, Yuquan Wei, Xiaorong Qi, Xiancheng Chen
There is an imperative choice to develop a secure feasible strategy to address evasion dynamics of refractory tumors and SARS-CoV-2-variants, while stem cell-based protocol may be more reliable as its unique ability for resetting multifunctional immunity to address progressive tumor and the constantly-evolving virus. In this study, spheroid-embryonoid stem cells from mature somatic cells were engineered
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Similar but distinct: The impact of biomechanical forces and culture age on the production, cargo loading, and biological efficacy of human megakaryocytic extracellular vesicles for applications in cell and gene therapies Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-06-22 Will Thompson, Eleftherios Terry Papoutsakis
Megakaryocytic extracellular vesicles (MkEVs) promote the growth and megakaryopoiesis of hematopoietic stem and progenitor cells (HSPCs) largely through endogenous miR-486-5p and miR-22-3p cargo. Here, we examine the impact of biomechanical force and culture age/differentiation on the formation, properties, and biological efficacy of MkEVs. We applied biomechanical force to Mks using two methods: shake
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From lab to society: Fostering clinical translation of molecular systems engineering Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-06-22 Renan Gonçalves Leonel da Silva, Alessandro Blasimme
Over the last decade, bioengineering has seen a sustained growth in scientific publications, patents, and clinical trials. As the field attempts to bridge the gap between discovery and clinical application, a broader societal dialogue is needed to build public trust and address potential ethical, societal, and regulatory challenges. In this essay, we discuss societal aspects linked to the clinical
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Automated microarray platform for single-cell sorting and collection of lymphocytes following HIV reactivation Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-06-21 Belén Cortés-Llanos, Vaibhav Jain, Alicia Cooper-Volkheimer, Edward P. Browne, David M. Murdoch, Nancy L. Allbritton
A promising strategy to cure HIV-infected individuals is to use latency reversing agents (LRAs) to reactivate latent viruses, followed by host clearance of infected reservoir cells. However, reactivation of latent proviruses within infected cells is heterogeneous and often incomplete. This fact limits strategies to cure HIV which may require complete elimination of viable virus from all cellular reservoirs
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A three-dimensional method for morphological analysis and flow velocity estimation in microvasculature on-a-chip Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-06-11 Alberto Rota, Luca Possenti, Giovanni S. Offeddu, Martina Senesi, Adelaide Stucchi, Irene Venturelli, Tiziana Rancati, Paolo Zunino, Roger D. Kamm, Maria Laura Costantino
Three-dimensional (3D) imaging techniques (e.g., confocal microscopy) are commonly used to visualize in vitro models, especially microvasculature on-a-chip. Conversely, 3D analysis is not the standard method to extract quantitative information from those models. We developed the μVES algorithm to analyze vascularized in vitro models leveraging 3D data. It computes morphological parameters (geometry
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Flourishing tumor organoids: History, emerging technology, and application Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-06-07 Qian Yang, Mengmeng Li, Xinming Yang, Zian Xiao, Xinying Tong, Ayinuer Tuerdi, Shisheng Li, Lanjie Lei
Malignant tumors are one of the leading causes of death which impose an increasingly heavy burden on all countries. Therefore, the establishment of research models that closely resemble original tumor characteristics is crucial to further understanding the mechanisms of malignant tumor development, developing safer and more effective drugs, and formulating personalized treatment plans. Recently, organoids
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Enhancing viability and angiogenic efficacy of mesenchymal stem cells via HSP90α and HSP27 regulation based on ROS stimulation for wound healing Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-06-07 Inwoo Seo, Sung-Won Kim, Jiyu Hyun, Yu-Jin Kim, Hyun Su Park, Jeong-Kee Yoon, Suk Ho Bhang
Light-based therapy has been reported as a potential preconditioning strategy to induce intracellular reactive oxygen species (ROS) signaling and improve the angiogenic properties of various types of cells. However, bio-stimulation mechanisms of light therapy in terms of ROS-heat shock proteins (HSPs) mediated anti-apoptotic and angiogenic pathways in human adult stem cells have not been fully delineated
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Light controlled self-escape capability of non-cationic carbon nitride-based nanosheets in lysosomes for hepatocellular carcinoma targeting stimulus-responsive gene delivery Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-06-06 Ming-Xuan Liu, Li Xu, Jia-Yi Jiang, Hai-Chen Dong, Peng-Fei Zhu, Lei Cao, Jing Chen, Xiao-Ling Zhang
High positive charge-induced toxicity, easy lysosomal degradation of nucleic acid drugs, and poor lesion sites targeting are major problems faced in the development of gene carriers. Herein, we proposed the concept of self-escape non-cationic gene carriers for targeted delivery and treatment of photocontrolled hepatocellular carcinoma (HCC) with sufficient lysosome escape and multiple response capacities
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Thermosensitive hydrogel-based GPR124 delivery strategy for rebuilding blood-spinal cord barrier Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-06-06 Jiawei Shu, Chenggui Wang, Yiqing Tao, Shaoke Wang, Feng Cheng, Yuang Zhang, Kesi Shi, Kaishun Xia, Ronghao Wang, Jingkai Wang, Chao Yu, Jiangjie Chen, Xianpeng Huang, Haibin Xu, Xiaopeng Zhou, Haobo Wu, Chengzhen Liang, Qixin Chen, Shigui Yan, Fangcai Li
Spinal cord injury (SCI) causes blood-spinal cord barrier (BSCB) disruption, leading to secondary damage, such as hemorrhagic infiltration, inflammatory response, and neuronal cell death. It is of great significance to rebuild the BSCB at the early stage of SCI to alleviate the secondary injury for better prognosis. Yet, current research involved in the reconstruction of BSCB is insufficient. Accordingly
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A simple and efficient strategy for cell-based and cell-free-based therapies in acute liver failure: hUCMSCs bioartificial liver Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-06-02 Lei Feng, Yi Wang, Yu Fu, Adilijiang Yimamu, Zeyi Guo, Chenjie Zhou, Shao Li, Linya Zhang, Jiasheng Qin, Shusong Liu, Xiaoping Xu, Zesheng Jiang, Shaoru Cai, Jianmin Zhang, Yang Li, Qing Peng, Xiao Yi, Guolin He, Ting Li, Yi Gao
Acute liver failure (ALF) is a life-threatening condition. Cell-based and cell-free-based therapies have proven to be effective in treating ALF; however, their clinical application is limited by cell tumorigenicity and extracellular vesicle (EV) isolation in large doses. Here, we explored the effectiveness and mechanism of umbilical cord mesenchymal stem cells (hUCMSCs)-based bioartificial liver (hUCMSC-BAL)
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Macrophage-based delivery of anti-fibrotic proteins alleviates bleomycin-induced pulmonary fibrosis in mice Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-06-02 Huiying Liu, Cuiping Yang, Yun Gao, Xueli Zhang, Min Wang, Xinting Yu, Weidong Wang, Lixin Xie, Ping Tang, Xiushan Yin, Changqing Bai, Luo Zhang
Idiopathic pulmonary fibrosis (IPF) is a fatal interstitial lung disease characterized by chronic, progressive, and fibrotic lung injury. Although remarkable progress has been made toward understanding the pathogenesis of PF, finding more effective treatments for this fatal disease remains a challenge. In this study, we describe an innovative macrophage-based approach to deliver anti-fibrotic protein
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Deep learning in precision medicine and focus on glioma Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-05-31 Yihao Liu, Minghua Wu
Deep learning (DL) has been successfully applied to different fields for a range of tasks. In medicine, DL methods have been also used to improve the efficiency of disease diagnosis. In this review, we first summarize the history of the development of artificial intelligence models, demonstrate the features of the subtypes of machine learning and different DL networks, and then explore their application
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Utilizing induced neural stem cell-based delivery of a cytokine cocktail to enhance chimeric antigen receptor-modified T-cell therapy for brain cancer Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-05-29 Alex S. Woodell, Elisa Landoni, Alain Valdivia, Andrew Buckley, Edikan A. Ogunnaike, Gianpietro Dotti, Shawn D. Hingtgen
Chimeric antigen receptor (CAR)-modified T-cell therapy has shown enormous clinical promise against blood cancers, yet efficacy against solid tumors remains a challenge. Here, we investigated the potential of a new combination cell therapy, where tumor-homing induced neural stem cells (iNSCs) are used to enhance CAR-T-cell therapy and achieve efficacious suppression of brain tumors. Using in vitro
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Novel piperazine-based ionizable lipid nanoparticles allow the repeated dose of mRNA to fibrotic lungs with improved potency and safety Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-05-28 Minjeong Kim, Michaela Jeong, Gyeongseok Lee, Yeji Lee, Jeongeun Park, Hyein Jung, Seongeun Im, Joo-Sung Yang, Kyungjin Kim, Hyukjin Lee
mRNA-based protein replacement therapy has received much attention as a novel intervention in clinical disease treatment. Lipid nanoparticles (LNPs) are widely used for their therapeutic potential to efficiently deliver mRNA. However, clinical translation has been hampered by the immunogenicity of LNPs that may aggravate underlying disease states. Here, we report a novel ionizable LNP with enhanced
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Basic research and clinical exploration of cold atmospheric plasma for skin wounds Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-05-26 Miaomiao Li, Jing Gao, Liyun Wang, Jia Liu, Chuyu Fu, Xingyu Yang, Shengquan Zhang, Xinwei Li, Shengyong Luo, Chunjun Yang
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Replenishable prevascularized cell encapsulation devices increase graft survival and function in the subcutaneous space Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-05-19 Gauree S. Chendke, Bhushan N. Kharbikar, Sudipta Ashe, Gaetano Faleo, Julie B. Sneddon, Qizhi Tang, Matthias Hebrok, Tejal A. Desai
Beta cell replacement therapy (BCRT) for patients with type 1 diabetes (T1D) improves blood glucose regulation by replenishing the endogenous beta cells destroyed by autoimmune attack. Several limitations, including immune isolation, prevent this therapy from reaching its full potential. Cell encapsulation devices used for BCRT provide a protective physical barrier for insulin-producing beta cells
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A high-throughput microfluidic mechanoporation platform to enable intracellular delivery of cyclic peptides in cell-based assays Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-05-13 Stephen H. Kasper, Stephanie Otten, Brian Squadroni, Cionna Orr-Terry, Yi Kuang, Lily Mussallem, Lan Ge, Lin Yan, Srinivasaraghavan Kannan, Chandra S. Verma, Christopher J. Brown, Charles W. Johannes, David P. Lane, Arun Chandramohan, Anthony W. Partridge, Lee R. Roberts, Hubert Josien, Alex G. Therien, Erik C. Hett, Bonnie J. Howell, Andrea Peier, Xi Ai, Jason Cassaday
Cyclic peptides are poised to target historically difficult to drug intracellular protein–protein interactions, however, their general cell impermeability poses a challenge for characterizing function. Recent advances in microfluidics have enabled permeabilization of the cytoplasmic membrane by physical cell deformation (i.e., mechanoporation), resulting in intracellular delivery of impermeable macromolecules
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Quatramer™ encapsulation of dual-targeted PI3-Kδ/HDAC6 inhibitor, HSB-510, suppresses growth of breast cancer Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-05-12 Sachchidanand Tiwari, Suiyang Liu, Mohd Anees, Neha Mehrotra, Ashish Thakur, Gregory J. Tawa, Gurmit Grewal, Richard Stone, Surender Kharbanda, Harpal Singh
Multiple studies have shown that the progression of breast cancer depends on multiple signaling pathways, suggesting that therapies with multitargeted anticancer agents will offer improved therapeutic benefits through synergistic effects in inhibiting cancer growth. Dual-targeted inhibitors of phosphoinositide 3-kinase (PI3-K) and histone deacetylase (HDAC) have emerged as promising cancer therapy
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An AIE-active bacterial inhibitor and photosensitizer for selective imaging, killing, and photodynamic inactivation of bacteria over mammalian cells Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-05-11 Fei Wang, Yupeng Shi, Po-Yu Ho, Engui Zhao, Chuen Kam, Qiang Zhang, Xin Zhao, Yue Pan, Sijie Chen
Photodynamic therapy is becoming increasingly popular for combat of bacteria. In the clinical photodynamic combat of bacteria, one critical issue is to avoid the potential damage to the host since the reactive oxygen species produced by photosensitizers are also harmful to mammalian cells. In this work, we report an aggregation-induced-emission-active bacterial inhibitor and photosensitizer, OEO-TPE-MEM
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Development and characterization of a recombinant silk network for 3D culture of immortalized and fresh tumor-derived breast cancer cells Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-05-11 Caterina Collodet, Kelly Blust, Savvini Gkouma, Emmy Ståhl, Xinsong Chen, Johan Hartman, My Hedhammar
Traditional cancer models rely on 2D cell cultures or 3D spheroids, which fail to recapitulate cell-extracellular matrix (ECM) interactions, a key element of tumor development. Existing hydrogel-based 3D alternatives lack mechanical support for cell growth and often suffer from low reproducibility. Here we report a novel strategy to make 3D models of breast cancer using a tissue-like, well-defined
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Decellularized squid mantle scaffolds as tissue-engineered corneal stroma for promoting corneal regeneration Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-05-09 Honghua Kang, Yi Han, Mengyi Jin, Lan Zheng, Zhen Liu, Yuhua Xue, Zuguo Liu, Cheng Li
Corneal blindness is a worldwide major cause of vision loss, and corneal transplantation remains to be the most effective way to restore the vision. However, often there is a shortage of the donor corneas for transplantation. Therefore, it is urgent to develop a novel tissue-engineered corneal substitute. The present study envisaged the development of a novel and efficient method to prepare the corneal
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Preclinical assessment of IRDye800CW-labeled gastrin-releasing peptide receptor-targeting peptide for near infrared-II imaging of brain malignancies Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-05-09 Yuan Zhang, Li Wang, Chengkai Zhang, Jingjing Zhang, Linhao Yuan, Shucheng Jin, Wenjianlong Zhou, Xiudong Guan, Peng Kang, Chuanbao Zhang, Jie Tian, Xiaoyuan Chen, Deling Li, Wang Jia
We aimed to develop a new biocompatible gastrin-releasing peptide receptor (GRPR) targeted optical probe, IRDye800-RM26, for fluorescence image-guided surgery (FGS) of brain malignancies in near-infrared window II (NIR-II) imaging. We developed a novel GRPR targeting probe using a nine-amino-acid bombesin antagonist analog RM26 combined with IRDye800CW, and explored the fluorescent probe according
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Drug cross-linking electrospun fiber for effective infected wound healing Bioeng. Transl. Med. (IF 7.4) Pub Date : 2023-05-05 Yuting Luo, Sen Zheng, Kun Wang, Hangqi Luo, Huiling Shi, Yanna Cui, Bingxin Li, Huacheng He, Jiang Wu
The management of infected wounds is still an intractable challenge in clinic. Development of antibacterial wound dressing is of great practical significance for wound management. Herein, a natural-derived antibacterial drug, tannic acid (TA), was incorporated into the electrospun polyvinyl alcohol (PVA) fiber (TA/PVA fiber, 952 ± 40 nm in diameter). TA worked as a cross-linker via hydrogen bonding