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Changes in conjunctival mononuclear phagocytes and suppressive activity of regulatory macrophages in desiccation induced dry eye Ocul. Surf. (IF 5.9) Pub Date : 2024-09-19 Jehan Alam, Ebru Yaman, Cintia S. de Paiva, De-Quan Li, Gerda Cristal Villalba Silva, Zhen Zuo, Stephen C. Pflugfelder
To evaluate the effects of dry eye on conjunctival immune cell number and transcriptional profiles with attention to mononuclear phagocytes.
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Epidemiology and risk factors for the development of cicatrizing conjunctivitis in chronic ocular graft-versus-host disease Ocul. Surf. (IF 5.9) Pub Date : 2024-09-12 Nicole B. Kantor, Paula A. Sepulveda-Beltran, David Valdés-Arias, Elyana V.T. Locatelli, Lakshman Mulpuri, Araliya N. Gunawardene, Guillermo Amescua, Victor L. Perez, Rahul Tonk, Trent Wang, Anat Galor
To evaluate the incidence of chronic cicatrizing conjunctivitis (CCC) and its associated risk factors in the context of chronic ocular graft-vs-host disease (coGVHD).
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Detection of SARS-CoV-2 binding receptors and miscellaneous targets as well as mucosal surface area of the human lacrimal drainage system Ocul. Surf. (IF 5.9) Pub Date : 2024-08-31 Anna-Lena Rau, Martin Schicht, Ingrid Zahn, Mohammad Javed Ali, Minas Theodore Coroneo, Friedrich Paulsen
Our aim was to evaluate a potential role for the lacrimal drainage system (LDS) as a portal of entry and conduit for SARS-CoV-2 in human infection. We also investigate the mucosal surface area. The relatively long tear contact time in a closed system raises the possibility that this pathway may contribute to the initiation of systemic infection. We looked for expression of ACE2, the main receptor for
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Readership awareness series – Paper 13: Key concepts of translational research Ocul. Surf. (IF 5.9) Pub Date : 2024-08-30 Mohammad Javed Ali MD PhD FRCS, Ali Djalilian MD
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Development and characterization of a preclinical mouse model of alkali-induced limbal stem cell deficiency Ocul. Surf. (IF 5.9) Pub Date : 2024-08-29 Lina Sprogyte, Mijeong Park, Lamia Nureen, Nicodemus Tedla, Alexander Richardson, Nick Di Girolamo
Limbal stem cell deficiency (LSCD) secondary to ocular surface alkali burn is a blinding condition that features corneal conjunctivalization. Mechanistic insights into its pathophysiology are lacking. Here, we developed a mouse model that recapitulates human disease to comprehensively delineate the clinicopathological features of a conjunctivalized cornea. LSCD was induced in the right eyes of 6-8-week-old
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Distinctive small molecules blend: Promotes lacrimal gland epithelial cell proliferation in vitro and accelerates lacrimal gland injury repair in vivo Ocul. Surf. (IF 5.9) Pub Date : 2024-08-28 Baihui Zeng, Lina Xu, Guoliang Wang, Ruize Shi, Kerui Wang, Shurong Wang, Cheng Li
This study aims to develop a novel serum-free culture strategy containing only two small molecules, Y27632 and SB431542 (2C), for expansion of mouse lacrimal gland epithelial cells (LGECs) and investigate an innovative therapeutic approach for lacrimal gland (LG) injury. LGECs proliferative capacity was assessed by cell counting, crystal violet staining, qRT-PCR and immunofluorescence. Cell differentiation
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β-Catenin gain of function mutant in mouse periocular neural crest-derived mesenchymal cells impairs embryonic eyelid morphogenesis and leads to blepharophimosis syndrome in mice Ocul. Surf. (IF 5.9) Pub Date : 2024-08-26 Yen-Chiao Wang, Yong Yuan, Jianhua Zhang, Yujin Zhang, Winston W.-Y. Kao, Chia-Yang Liu
The aberrant canonical Wnt-β-catenin signaling can cause devastating outcomes of tissue morphogenesis and tumor formation. In this study, we examined the impact of overexpression of constitutive active β-catenin in mouse periocular neural crest-derived mesenchymal cells during embryonic eyelid morphogenesis. We expressed a stabilized β-catenin in which the exon 3 of the 1 gene was deleted in periocular
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Serum-derived extracellular vesicles for the treatment of severe ocular surface disease Ocul. Surf. (IF 5.9) Pub Date : 2024-08-17 Namita Saraf, Rajalakshmy Ayilam Ramachandran, Mou Cao, Andrew Lemoff, Hamid Baniasadi, Danielle M. Robertson
Autologous serum is widely used for the treatment of severe ocular surface disease with mixed efficacy. Extracellular vesicles (EVs) are small membrane bound structures present in all body fluids, including serum. This study compared the proteomic, metabolomic, and inflammatory cytokine composition of serum-derived EVs (SDEVs) to that of the soluble free protein fraction and the subsequent capacity
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Tear neuropeptide Y as a non-invasive marker of peripheral microvascular complications in type 1 diabetes Ocul. Surf. (IF 5.9) Pub Date : 2024-08-15 Alexis Ceecee Britten-Jones, Mengliang Wu, Leslie J. Roberts, Richard J. MacIsaac, Haihan Jiao, Jennifer P. Craig, Holly R. Chinnery, Laura E. Downie
To investigate tear neuropeptide Y (NPY) and substance P concentrations in individuals with type 1 diabetes, comparing those with and without both diabetic retinopathy (DR) and peripheral neuropathy. This cross-sectional study involved 41 participants with type 1 diabetes and none to moderate DR, and 22 healthy controls. Assessments included clinical ocular surface parameters, quantification of corneal
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The clinical outcomes of minor salivary gland transplantation for severe dry eye disease secondary to chronic Stevens-Johnson syndrome Ocul. Surf. (IF 5.9) Pub Date : 2024-08-14 Namrata Sharma, Vishal Kumar, Aafreen Bari, Renu Venugopal, Shivam Sharma, Tushar Agarwal, Tanuj Dada, Neelam Pushker
To study the outcomes of minor salivary gland transplantation for severe dry eye disease secondary to chronic Steven Johnson Syndrome. It was an ambispective, interventional case series conducted at Rajendra Prasad Centre for Ophthalmic Sciences, Delhi, India from 2022 to 2023 evaluating the outcomes of minor salivary gland transplantation with anchorage of the minor salivary glands to superior rectus
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Punctal cautery in dry eye disease: A systematic review Ocul. Surf. (IF 5.9) Pub Date : 2024-08-09 Ashish Ranjan, Sayan Basu, Swati Singh
To critically appraise the evidence on the efficacy and recanalization rates of permanent punctal occlusion via thermal or surgical means in managing dry eye disease (DED). In PubMed, Scopus, and Cochrane databases, two authors systematically reviewed the literature for prospective studies on punctal cautery or surgical occlusion (excluding punctal plugs) for DED. The studied outcomes were changes
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Development and validation of a method to generate phenol red thread tests Ocul. Surf. (IF 5.9) Pub Date : 2024-08-08 Bryan Puentes, Erin A. Hisey, Michelle Ferneding, Vanessa N. Ureno, Maria A.H. Do, Paige M. Karpinen, Chung-Chih Luo, Sara M. Thomasy, Brian C. Leonard
The aim of this study was to develop and validate a method to generate phenol red thread tests (PRTT) due to the lack of availability of commercial PRTT. White cotton thread was dyed with phenol red (pH indicator) for 48 h, dried, cut, and sterilized. To validate its wicking ability, the thread was inserted into solutions of varying pH, flanking the pH of healthy tears, for different time intervals
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Duloxetine enhances PAX6 expression and suppresses innate immune responses in murine LPS-induced corneal inflammation Ocul. Surf. (IF 5.9) Pub Date : 2024-08-08 Petros Moustardas, Mojdeh Abbasi, Dina Javidjam, Cindy Saah Asamoah, Arnaud Schweitzer-Chaput, Salvatore Cisternino, Dominique Bremond-Gignac, Daniel Aberdam, Neil Lagali
is a key regulator of eye development and epithelial homeostasis in the cornea. When deficient, chronic corneal inflammation, neovascularization and limbal stem cell deficiency can occur. Here we investigated the potential of duloxetine, a generic serotonin reuptake inhibitor that can upregulate PAX6 , for its activity in the context of corneal inflammation. Duloxetine tolerance was tested in a human
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The effects of age and dysfunction on meibomian gland population dynamics Ocul. Surf. (IF 5.9) Pub Date : 2024-08-08 Julie Wiedemann, Ghaidaa Kashgari, Shelley Lane, Brian C. Leonard, Kelly E. Knickelbein, Bogi Andersen, James V. Jester
While meibomian gland dysfunction (MGD) is widely recognized as a major cause of evaporative dry eye disease, little is known about normal gland differentiation and lipid synthesis or the mechanism underlying gland atrophy and abnormal lipid secretion. The purpose of this study was to use single-cell and spatial transcriptomics to probe changes in cell composition, differentiation, and gene expression
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Pipeline: Wiley Chambers, MD, FDA Ophthalmology regulator Ocul. Surf. (IF 5.9) Pub Date : 2024-08-08 Gary D. Novack
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Evolution of therapeutic strategy based on oxidant-antioxidant balance for fuchs endothelial corneal dystrophy Ocul. Surf. (IF 5.9) Pub Date : 2024-08-05 Yiming Wu, Yanbo Liu, Yuchong Feng, Xiaoshuang Li, Zhaoxiang Lu, Hao Gu, Wei Li, Lisa J. Hill, Shangkun Ou
Fuchs endothelial corneal dystrophy (FECD) stands as the most prevalent primary corneal endothelial dystrophy worldwide, posing a significant risk to corneal homeostasis and clarity. Corneal endothelial cells exhibit susceptibility to oxidative stress, suggesting a nuanced relationship between oxidant-antioxidant imbalance and FECD pathogenesis, irrespective of FECD genotype. Given the constrained
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Topical anti-glaucoma medications effects on meibomian glands: Systematic review and meta-analysis Ocul. Surf. (IF 5.9) Pub Date : 2024-08-05 Bruna Grici Cascaldi, Antero Pinto Cardoso Filho, Gustavo Viani Arruda, Eduardo Melani Rocha, Jayter Silva Paula
Meibomian gland (MG) dysfunction and glaucoma are very prevalent ocular conditions that significantly impact patients’ quality of life. A growing number of clinical and experimental studies have reported an association between the use of topical eye drops to reduce intraocular pressure and the development or exacerbation of MG dysfunction. Studies investigating the impact of glaucoma eyedrops on MG
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Preservatives and ocular surface disease: A review Ocul. Surf. (IF 5.9) Pub Date : 2024-08-03 Malik Y. Kahook, Christopher J. Rapuano, Elisabeth M. Messmer, Nathan M. Radcliffe, Anat Galor, Christophe Baudouin
Ocular surface disease (OSD) is a complex condition that can cause a range of symptoms (e.g, dryness, irritation, and pain) and can significantly impact the quality of life of affected individuals. Iatrogenic OSD, a common finding in patients with glaucoma who receive chronic therapy with topical ocular antihypertensive drugs containing preservatives such as benzalkonium chloride (BAK), has been linked
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Unraveling the complexity – Insights and interventions of refractory vernal keratoconjunctivitis Ocul. Surf. (IF 5.9) Pub Date : 2024-08-03 Shweta Agarwal, Serge Doan, Bhaskar Srinivasan, Geetha Iyer, Andrea Leonardi
Vernal keratoconjunctivitis (VKC) is a chronic severe ocular allergic inflammation mostly observed in children and young adults. The ocular manifestations are the expression of multifactorial immune mechanisms that generally have a good prognosis, however long-term inflammation may remarkably reduce the visual function due to complications and poor therapeutic responses.
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Deep-learning based analysis of in-vivo confocal microscopy images of the subbasal corneal nerve plexus’ inferior whorl in patients with neuropathic corneal pain and dry eye disease Ocul. Surf. (IF 5.9) Pub Date : 2024-08-02 Raul E. Ruiz-Lozano, Matias Soifer, Zane Z. Zemborain, Nadim S. Azar, Manuel E. Quiroga-Garza, Sofia Murillo, Symon Ma, Seitaro Komai, Anupama Horne, Ali Khodor, Luis A. Rodriguez-Gutierrez, Sandra S. Stinnett, Sina Farsiu, Victor L. Perez
To evaluate and compare subbasal corneal nerve parameters of the inferior whorl in patients with dry eye disease (DED), neuropathic corneal pain (NCP), and controls using a novel deep-learning-based algorithm to analyze in-vivo confocal microscopy (IVCM) images. Subbasal nerve plexus (SNP) images of the inferior whorl of patients with DED (n = 49, 77 eyes), NCP (n = 14, 24 eyes), and controls (n = 41
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Readership awareness series – Paper 12: Clinician-scientist – Threats to the endangered species and preservation strategies Ocul. Surf. (IF 5.9) Pub Date : 2024-07-30 Mohammad Javed Ali, Ali Djalilian
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A novel artificial intelligence model for diagnosing Acanthamoeba keratitis through confocal microscopy Ocul. Surf. (IF 5.9) Pub Date : 2024-07-29 Omar Shareef, Mohammad Soleimani, Elmer Tu, Deborah Jacobs, Joseph Ciolino, Amir Rahdar, Kasra Cheraqpour, Mohammadali Ashraf, Nabiha B. Habib, Jason Greenfield, Siamak Yousefi, Ali R. Djalilian, Hajirah N. Saeed
To develop an artificial intelligence (AI) model to diagnose keratitis (AK) based on confocal microscopy (IVCM) images extracted from the Heidelberg Retinal Tomograph 3 (HRT 3). This retrospective cohort study utilized HRT 3 IVCM images from patients who had received a culture-confirmed diagnosis of AK between 2013 and 2021 at Massachusetts Eye and Ear. Two cornea specialists independently labeled
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Demographic and lifestyle factors associated with dry eye disease in China: A cross-sectional multi-center study Ocul. Surf. (IF 5.9) Pub Date : 2024-07-27 Wanju Yang, Haoyu Wang, Jinghua Li, Yuan Chen, Yu Zhang, Xiaoxia Niu, Xiujun Song, Yuhong Tang, Dan Chen, Honglin Han, Hai Huang, Ying Bao, Xiaofeng Li, Jianzhang Ye, Yu Zheng, Xiangxi Chen, Chang Liu, Congxiang Wang, Tiehong Chen, Bin Yu, Jingyan Yan, Kuiliang Yang, Han Zhang, Qingyan Zeng, Shaowei Li
Associations were assessed between demographic/lifestyle factors and tear film breakup time (TBUT) defined dry eye disease (DED) in China. The cross-sectional study involved 50,280 subjects (54 ± 17 y) in 217 clinics (25 provinces). Data included sleep disorders; digital screen exposure; and use of cosmetics, contact lenses, and eye drops (for asthenopia). Clinical examinations included TBUT; Schirmer
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Remission of corneal and peripheral neuropathy after bariatric surgery in people with diabetes Ocul. Surf. (IF 5.9) Pub Date : 2024-07-23 Stuti L. Misra, James A. Slater, Rahul Makam, Geoffrey D. Braatvedt, Grant Beban, Monika Pradhan, Joseph L. Mankowski, Jonathan D. Oakley, Charles N.J. McGhee
Diabetic peripheral neuropathy can be detected using non-invasive confocal microscopy of the cornea (IVCM) and such abnormalities may precede the development of clinical neuropathy. The current study aimed to assess any progression or remission of corneal and peripheral neuropathy in patients with type 2 diabetes undergoing bariatric surgery. People with known type 2 diabetes for at least five years
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The roles of neural adaptation and sensitization in contact lens discomfort Ocul. Surf. (IF 5.9) Pub Date : 2024-07-22 Ping Situ, Carolyn Begley, Trefford Simpson
To investigate the roles of neural adaptation and sensitization in contact lens discomfort (CLD). Cooling stimuli (20 °C) were applied to the cornea in a group comprising 24 symptomatic and 25 asymptomatic contact lens (CL) wearers as well as 15 non-CL wearing controls, using a computerized Belmonte esthesiometer. The adaptation paradigm consisted of 20 repetitive stimuli at threshold, sub- and supra-threshold
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Dry eye disease adverse reaction of pharmacological treatment for early-stage breast cancer Ocul. Surf. (IF 5.9) Pub Date : 2024-07-20 Jiamu He, Yifan Zhou, Meng Xiu, Yiyun Liu, Baikai Ma, Hongyu Duan, Jiawei Chen, Lu Zhao, Qianqian Lan, Yunke Ma, Xiaoyu Wang, Hong Qi
Breast cancer is the most prevalent cancer worldwide. With advancements in breast cancer diagnosis and treatment, the prognosis of patients with early-stage cancer has significantly improved. Enhancing the long-term quality of life of patients after antineoplastic therapy, including visual quality, has become a crucial research focus. This review aims to comprehensively summarize dry eye disease adverse
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Discriminative performance of ocular surface staining and lid wiper epitheliopathy in dry eye disease: An investigator-masked, prospective registry-based, diagnostic accuracy study Ocul. Surf. (IF 5.9) Pub Date : 2024-07-20 Michael T.M. Wang, Barry Power, Ally L. Xue, Jennifer P. Craig
To evaluate the diagnostic performance of corneal and conjunctival staining, and lid wiper epitheliopathy (LWE) in detecting dry eye disease, as defined by the global consensus Tear Film and Ocular Surface Society Dry Eye Workshop II (TFOS DEWS II) criteria. A total of 2066 community residents (1285 females; mean ± SD age, 40 ± 19 years) were recruited in an investigator-masked, prospective registry-based
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Toxicity and efficacy of type I interferons on the ocular surface: in vitro, animal, and clinical studies Ocul. Surf. (IF 5.9) Pub Date : 2024-07-11 Young In Yun, Jung Hwa Ko, Jin Suk Ryu, Seonghwan Kim, Hyun Sun Jeon, Namju Kim, Mee Kum Kim, Joo Youn Oh
To investigate the toxicity of type I interferons (IFNs) on the ocular surface and assess their efficacy in ocular surface tumors. We examined the effects of IFN-α2a, IFN-α2b and IFN-β on corneal epithelial cells and stromal fibroblasts as well as the impact of IFN-α2a on the ocular surface in mice. Additionally, we analyzed the therapeutic and adverse effects of topically administered IFN-α2a and
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Mpox Virus and its ocular surface manifestations Ocul. Surf. (IF 5.9) Pub Date : 2024-07-05 Nikhil Chakravarty, Darshi Hemani, Ramya Paravastu, Zeeshan Ahmad, Sankara Naynar Palani, Vaithilingaraja Arumugaswami, Ashok Kumar
The Mpox virus (MPXV) is the causative agent of human Mpox disease – a debilitating rash illness similar to smallpox. Although Clade I MPXV has remained endemic to West and Central Africa, Clade II MPXV has been responsible for many outbreaks worldwide. The most recent outbreak in 2022 resulted from the rapid spread of a new clade of MPXV, classified into Clade IIb – a distinct lineage from the previously
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Analysis of the heterogeneity and complexity of murine extraorbital lacrimal gland via single-cell RNA sequencing Ocul. Surf. (IF 5.9) Pub Date : 2024-06-28 Duliurui Huang, Xinwei Jiao, Shenzhen Huang, Jiangman Liu, Hongli Si, Di Qi, Xiaoting Pei, Dingli Lu, Yimian Wang, Zhijie Li
The lacrimal gland is essential for maintaining ocular surface health and avoiding external damage by secreting an aqueous layer of the tear film. However, a healthy lacrimal gland's inventory of cell types and heterogeneity remains understudied. Here 10X Genome-based single-cell RNA sequencing was used to generate an unbiased classification of cellular diversity in the extraorbital lacrimal gland
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Whole mount immunofluorescence analysis of fresh and stored human donor corneas highlights changes in limbal characteristics during storage Ocul. Surf. (IF 5.9) Pub Date : 2024-06-28 Maija Kauppila, Meri Vattulainen, Teemu O. Ihalainen, Anni Mörö, Tanja Ilmarinen, Heli Skottman
Human donor corneas are an essential control tissue for corneal research. We utilized whole mount immunofluorescence (WM-IF) to evaluate how the storage affects the tissue integrity and putative limbal stem cells in human and porcine corneas. Moreover, we compare this information with the marker expression patterns observed in human pluripotent stem cell (hPSC)-derived LSCs. The expression of putative
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Restoration of corneal epithelial barrier function: A possible target for corneal neovascularization Ocul. Surf. (IF 5.9) Pub Date : 2024-06-18 Sitong Shen, Yan Zhang
Corneal neovascularization (CoNV) is the second leading common cause of vision impairment worldwide and is a blinding pathological alteration brought on by ocular trauma, infection, and other factors. There are some limitations in the treatment of CoNV, hence it's critical to look into novel therapeutic targets. The corneal epithelial barrier, which is the initial barrier of the ocular surface, is
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Calcium-sensing receptor (CaSR) modulates ocular surface chloride transport and its inhibition promotes ocular surface hydration Ocul. Surf. (IF 5.9) Pub Date : 2024-06-11 Neel D. Pasricha, Ethan S. Lindgren, Rongshan Yan, Yien-Ming Kuo, Matilda Chan, Alan S. Verkman, Tifany Chu, Pattareeya Yottasan, Livia de Souza Goncalves, Onur Cil
Ocular surface hydration is critical for eye health and its impairment can lead to dry eye disease. Extracellular calcium-sensing receptor (CaSR) is regulator of ion transport in epithelial cells expressing cystic fibrosis transmembrane conductance regulator (CFTR) Cl channel. CFTR is also a major ion channel in ocular surface epithelia, however the roles of CaSR in ocular surface are not well studied
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Filamentary keratitis: A review Ocul. Surf. (IF 5.9) Pub Date : 2024-06-08 Aravind Roy, Smruti Rekha Priyadarshini, Sujata Das
Filamentary keratitis (FK) is a clinical sign of underlying ocular and systemic conditions. FK can cause significant irritation, tearing, and photophobia in the eye. It is a refractory debilitating condition caused by dry eye that affects the day-to-day activities of patients. The etiopathogenesis of FK is not well known; there are numerous predisposing causes. The condition starts as a sub-epithelial
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A novel severity scoring system for murine ocular graft versus host disease and its correlation with CD3+ T cells in the cornea Ocul. Surf. (IF 5.9) Pub Date : 2024-05-31 Fernando Martinez Guasch, Seema Sajjan, Ellis Tibbs, Cassidy Reandeau, Long Wu, Jerry Bohlen, Andrew Li, Amy Plotkin, Xuefang Cao, Sarah B. Sunshine
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Delayed diagnosis of ocular graft-versus-host disease after allogeneic hematopoietic stem cell transplantation Ocul. Surf. (IF 5.9) Pub Date : 2024-05-29 Yinglin Liao, Wenxin Zhao, Jing Yang, Jing Li, Juejing Chen, Ziyan Chen, Ling Jin, Longyue Li, Fen Huang, Lingyi Liang
To investigate the delayed diagnosis of chronic ocular graft-versus-host disease (coGVHD) after allogeneic hematopoietic stem cell transplantation (alloHCT), and further analyze potential confounding factors. This cross-sectional study included 118 patients newly diagnosed as coGVHD after alloHCT at Zhongshan Ophthalmic Center, Sun Yat-sen University. All participants finished the flow path of medical
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Pipeline: Decoding the package insert: Adverse events revisited Ocul. Surf. (IF 5.9) Pub Date : 2024-05-22 Gary D. Novack
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Effects of age on lacrimal gland bioactive lipids Ocul. Surf. (IF 5.9) Pub Date : 2024-05-04 Brandon Ebright, Zhiyuan Yu, Priyal Dave, Dante Dikeman, Sarah Hamm-Alvarez, Cintia S. de Paiva, Stan Louie
Polyunsaturated fatty acids (PUFA) are a source of bioactive lipids regulating inflammation and its resolution. Changes in PUFA metabolism were compared between lacrimal glands (LGs) from young and aged C57BL/6 J mice using a targeted lipidomics assay, as was the gene expression of enzymes involved in the metabolism of these lipids. Global reduction in PUFAs and their metabolites was observed in aged
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Elevated neutrophils and reduced NK cells are associated with altered tear molecular signatures and clinical sequelae of chronic ocular Stevens-Johnson syndrome Ocul. Surf. (IF 5.9) Pub Date : 2024-05-03 Sharon D'Souza, Archana Padmanabhan Nair, Nikhil Ashok, Ramaraj Kannan, Mor M. Dickman, Rudy M.M.A. Nuijts, Rohit Shetty, Swaminathan Sethu, Arkasubhra Ghosh
Stevens-Johnson syndrome (SJS) is characterised as an immuno-inflammatory condition with potentially blinding ocular sequelae. Therefore, we have investigated the ocular surface immune cell profile and correlated it with secreted tear molecular factors and clinical ocular sequelae in SJS patients. 21 patients (42 eyes) with chronic ocular SJS and 16 healthy controls (20 eyes) were included in the study
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Single-cell RNA-sequencing reveals the transcriptional landscape of lacrimal gland in GVHD mouse model Ocul. Surf. (IF 5.9) Pub Date : 2024-05-03 Jingliang He, Fang Zheng, Li Zhang, Jiangxiong Cai, Yoko Ogawa, Kazuo Tsubota, Shan Liu, Xiuming Jin
To investigate the global transcriptional landscape of lacrimal gland cell populations in the GVHD mouse model. Single-cell RNA sequencing and further bioinformatic analysis of dissociated lacrimal gland (LG) cells from the mouse model were performed. Parts of transcriptional results were confirmed by immunofluorescence staining. We identified 23 cell populations belonging to 11 cell types. In GVHD
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Evaluating the efficacy and safety of therapeutic interventions for corneal neuropathy: A systematic review Ocul. Surf. (IF 5.9) Pub Date : 2024-04-28 Rajni Rajan, Eve Makrai, Ji-hyun Lee, Sumeer Singh, Holly R. Chinnery, Laura E. Downie
Corneal neuropathy involves corneal nerve damage that disrupts ocular surface integrity, negatively impacting quality-of-life from pain and impaired vision. Any ocular or systemic condition that damages the trigeminal nerve can lead to corneal neuropathy. However, the condition currently does not have standardized diagnostic criteria or treatment protocols. The primary aim of this systematic review
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Readership awareness series – Paper 11: Copyright licensing for scientific publications Ocul. Surf. (IF 5.9) Pub Date : 2024-04-28 Mohammad Javed Ali, Ali Djalilian
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HDAC1/2 and HDAC3 play distinct roles in controlling adult Meibomian gland homeostasis Ocul. Surf. (IF 5.9) Pub Date : 2024-04-26 Xuming Zhu, Mingang Xu, Sarah E. Millar
To investigate the roles of HDAC1/2 and HDAC3 in adult Meibomian gland (MG) homeostasis. HDAC1/2 or HDAC3 were inducibly deleted in MG epithelial cells of adult mice. The morphology of MG was examined. Proliferation, apoptosis, and expression of MG acinus and duct marker genes, meibocyte differentiation genes, and HDAC target genes, were analyzed via immunofluorescence, TUNEL assay, and RNA in situ
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Corrigendum to “New insights into lacrimal gland anatomy using 7T MRI and electron microscopy: Relevance for lacrimal gland targeted therapies and bioengineering” [Ocul. Surf. 30 (October 2023) 204–212] Ocul. Surf. (IF 5.9) Pub Date : 2024-04-16 Swati Singh, Zoltan Winter, Fabian Necker, Tobias Bäuerle, Michael Scholz, Lars Bräuer, Friedrich Paulsen
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Readership awareness series – Paper 10: Open Access Publishing Ocul. Surf. (IF 5.9) Pub Date : 2024-04-16 Mohammad Javed Ali, Ali Djalilian
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Comparative analysis of human tear fluid and aqueous humor proteomes Ocul. Surf. (IF 5.9) Pub Date : 2024-03-30 August Beisel, Garrett Jones, Joshua Glass, Tae Jin Lee, Marc Töteberg-Harms, Amy Estes, Lane Ulrich, Kathryn Bollinger, Shruti Sharma, Ashok Sharma
Technological advancements allowing for the analysis of low-volume samples have led to the investigation of human tear fluid and aqueous humor (AH) as potential biomarker sources. However, acquiring AH samples poses significant challenges, making human tear fluid a more accessible alternative. This study aims to compare the protein compositions of these two biofluids to evaluate their suitability for
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Predictive performance of corneal and lid margin sensitivity for dry eye disease: An investigator-masked, prospective, prognostic accuracy study Ocul. Surf. (IF 5.9) Pub Date : 2024-03-28 Michael T.M. Wang, Jay J. Meyer, Ally L. Xue, Barry Power, Jennifer P. Craig
To evaluate the prognostic ability of non-contact esthesiometry corneal and lid margin sensitivity measurements in detecting symptoms and signs of dry eye disease, as defined by the global consensus TFOS DEWS II criteria. A total of 87 community residents (58 females; mean ± SD age, 53 ± 16 years) were recruited in an investigator-masked, prospective, prognostic accuracy study. Dry eye symptomology
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New disposable esthesiometer (KeraSenseⓇ) to improve diagnosis and management of neurotrophic keratitis Ocul. Surf. (IF 5.9) Pub Date : 2024-03-21 Francesca Giovannetti, Marta Sacchetti, Marco Marenco, Ludovico Alisi, Giacomo Visioli, Alice Bruscolini, Alessandro Lambiase
To validate the use, repeatability, and reproducibility of a new, cost-effective, disposable, sterile device (KeraSenseⓇ, Dompè farmaceutici SpA, Milan Italy) compared to Cochet-Bonnet (CB) esthesiometer. Secondly, to identify a simple, safe, rapid, and low-cost test to diagnose neurotrophic keratitis (NK). 16 patients with diagnosis of NK stage I, 25 patients with diabetes mellitus (DM), and 26 healthy
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New-onset keratitis associated with epidermal growth factor receptor-based targeted therapies in Han Chinese patients with lung cancer: A multi-center cohort study Ocul. Surf. (IF 5.9) Pub Date : 2024-03-19 Kevin Sheng-Kai Ma, Jui-En Lo, James Chodosh, Reza Dana
To determine the risk and incidence of keratitis following treatment with epidermal growth factor receptor inhibitors (EGFRi) and subtypes of EGFRi-associated keratitis. This multi-center cohort study included EGFRi-treated patients and non-users with lung cancer between 2010 and 2023. EGFRi included first-generation agent gefitinib and erlotinib, second-generation agent afatinib, and third-generation
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Cellular senescence promotes meibomian gland dysfunction in a chronic graft-versus-host disease mouse model Ocul. Surf. (IF 5.9) Pub Date : 2024-03-16 Shinri Sato, Yoko Ogawa, Eisuke Shimizu, Kazuki Asai, Takahiro Okazaki, Robert Rusch, Masatoshi Hirayama, Shigeto Shimmura, Kazuno Negishi, Kazuo Tsubota
Aging is a well-established risk factor for meibomian gland dysfunction (MGD). We previously reported an accelerated cellular senescence phenomenon in the lacrimal glands of a murine model of chronic graft-versus-host disease (cGVHD). Herein, we aimed to elucidate the relationship between cellular senescence and MGD in cGVHD mice, utilizing the senolytic agent ABT-263. A cGVHD mouse model was established
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Optimising subjective grading of corneal staining in Sjögren's syndrome dry eye disease Ocul. Surf. (IF 5.9) Pub Date : 2024-03-14 James S. Wolffsohn, Alberto Recchioni, Olivia A. Hunt, Sònia Travé-Huarte, Giuseppe Giannaccare, Marco Pellegrini, Marc Labetoulle
To assess whether smaller increment and regionalised subjective grading improves the repeatability of corneal fluorescein staining assessment, and to determine the neurological approach adopted for subjective grading by practitioners. Experienced eye-care practitioners (n = 28, aged 45 ± 12 years), graded 20 full corneal staining images of patients with mild to severe Sjögren's syndrome with the Oxford
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Unique cytokine signature in ocular Stevens-Johnson syndrome non-responders Ocul. Surf. (IF 5.9) Pub Date : 2024-03-13 Srividya Gurumurthy, Bhaskar Srinivasan, Shweta Agarwal, Hiren Matai, Narayanasamy Angayarkanni, Geetha Iyer
To clinically define a subset of patients with chronic ocular Stevens-Johnson syndrome non-responders (SJS-NR) and analyze their cytokine profile compared to clinical responders (SJS-CR) A total of 32 SJS cases (n = 32, 64 eyes) managed over a period of three years were segregated into clinical responders (n = 24, 48 eyes) and non-responders (n = 8, 16 eyes). Cases were determined as non-responders
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Tear cytokine levels are reduced in patients treated with intravitreal injections Ocul. Surf. (IF 5.9) Pub Date : 2024-03-13 Agni Malmin, Vilde M. Thomseth, Per T. Førland, Hans C.D. Aass, Sjur Reppe, Markus V.T. Olsen, Birger Lindtjørn, Xiangjun Chen, Inga B.K. Haugen, Tor P. Utheim, Vegard A. Forsaa
To investigate cytokine levels in the tear fluid of patients receiving serial intravitreal injections (IVI) with anti-vascular endothelial growth factor (anti-VEGF) for neovascular age-related macular degeneration (nAMD). Concentrations of six cytokines (IFN-γ, IL-1β, IL-6, IL-8, TNF and VEGF) in tears of patients receiving anti-VEGF in one eye were assayed using multiplex cytometric bead array. The
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Ectoine protects corneal epithelial survival and barrier from hyperosmotic stress by promoting anti-inflammatory cytokine IL-37 Ocul. Surf. (IF 5.9) Pub Date : 2024-03-13 Jin-Miao Li, Na Lin, Yun Zhang, Xin Chen, Zhao Liu, Rong Lu, Fang Bian, Haixia Liu, Stephen C. Pflugfelder, De-Quan Li
To explore novel role and molecular mechanism of a natural osmoprotectant ectoine in protecting corneal epithelial cell survival and barrier from hyperosmotic stress. Primary human corneal epithelial cells (HCECs) were established from donor limbus. The confluent cultures in isosmolar medium were switched to hyperosmotic media (400–500 mOsM), with or without ectoine or rhIL-37 for different time periods
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Models for Meibomian gland dysfunction: In vivo and in vitro Ocul. Surf. (IF 5.9) Pub Date : 2024-03-13 Jinghua Bu, Yuli Guo, Yang Wu, Rongrong Zhang, Jingbin Zhuang, Jiankai Zhao, Le Sun, Andrew J. Quantock, Zuguo Liu, Wei Li
Meibomian gland dysfunction (MGD) is a chronic abnormality of the Meibomian glands (MGs) that is recognized as the leading cause of evaporative dry eye worldwide. Despite its prevalence, however, the pathophysiology of MGD remains elusive, and effective disease management continues to be a challenge. In the past 50 years, different models have been developed to illustrate the pathophysiological nature
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Effect of cosmetics on the measurement of the tear lipid layer thickness by LipiView® Ocul. Surf. (IF 5.9) Pub Date : 2024-03-12 Young Chae Yoon, Sun Kyung Park, Yoo-Sun Sung, Ji Young Park, Woong Joo Whang, Kyung Sun Na, Eun Chul Kim, Hyun Seung Kim, Ho Sik Hwang
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Electroencephalogram-detected stress levels in the frontal lobe region of patients with dry eye Ocul. Surf. (IF 5.9) Pub Date : 2024-03-07 Minako Kaido, Reiko Arita, Yasue Mitsukura, Kazuo Tsubota
To evaluate stress levels extracted from prefrontal electroencephalogram (EEG) signals and investigate their relationship with dry eye symptoms. This prospective, cross-sectional, comparative study included 25 eyes of 25 patients with aqueous tear-deficient dry eye (low Schirmer group), 25 eyes of 25 patients with short tear breakup time dry eye (short breakup time group), and 24 eyes of 24 individuals
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Chronic inflammation as a proposed risk factor for ocular surface squamous neoplasia Ocul. Surf. (IF 5.9) Pub Date : 2024-03-01 Sofia De Arrigunaga, Sarah Wall, Despoina Theotoka, Asaf Friehmann, Matthew Camacho, Sander Dubovy, Anat Galor, Carol L. Karp
Chronic inflammation is a predisposing factor for metaplastic changes and ultimately dysplasia. We describe cases of OSSN occurring in the setting of chronic ocular surface inflammation. Sixteen eyes from 14 individuals were included from one ocular oncology clinic between 2010 and 2023. Patients presented with ocular surface squamous neoplasia (OSSN) in the setting of chronic inflammation. The diagnosis
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Visual outcomes of children undergoing penetrating keratoplasty in the US Ocul. Surf. (IF 5.9) Pub Date : 2024-02-24 Lyvia J. Zhang, Reza Dana, Alice C. Lorch, Tobias Elze, Joan W. Miller, Thomas H. Dohlman, Isdin Oke, IRIS®Registry Analytic Center Consortium
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Immunologic basis for development of keratoconjunctivitis sicca in systemic autoimmune diseases: Role of innate immune sensors Ocul. Surf. (IF 5.9) Pub Date : 2024-02-22 Michael E. Stern, Argyrios N. Theofilopoulos, Philipp Steven, Jerry Y. Niederkorn, Robert Fox, Margarita Calonge, Christof Scheid, Stephen C. Pflugfelder
The literature is filled with citations reporting an increased incidence of chronic dry eye disease, also known as keratoconjunctivitis sicca, in patients with systemic autoimmune diseases such as rheumatoid arthritis, Sjögren's Syndrome, systemic sclerosis and lupus. As the most environmentally exposed mucosal surface of the body, the conjunctiva constantly responds to environmental challenges which