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Circulating Tumor DNA as a Biomarker for Monitoring Patients with Solid Cancers: Comparison with Standard Protein Biomarkers Clin. Chem. (IF 12.167) Pub Date : 2022-08-13 Michael J Duffy, John Crown
Background Protein-based biomarkers are widely used in monitoring patients with diagnosed cancer. These biomarkers however, lack specificity for cancer and have poor sensitivity in detecting early recurrences and monitoring therapy effectiveness. Emerging data suggest that the use of circulating tumor DNA (ctDNA) has several advantages over standard biomarkers. Content Following curative-intent surgery
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Detection of Circulating Tumor Cells in Cerebrospinal Fluid of Patients with Suspected Breast Cancer Leptomeningeal Metastases: A Prospective Study Clin. Chem. (IF 12.167) Pub Date : 2022-08-12 Amélie Darlix, Laure Cayrefourcq, Stéphane Pouderoux, Nicolas Menjot de Champfleur, Alexis Bievelez, William Jacot, Cristina Leaha, Simon Thezenas, Catherine Alix-Panabières
Background The diagnosis of breast cancer (BC)-related leptomeningeal metastases (LM) relies on the detection of tumor cells in cerebrospinal fluid (CSF) using conventional cytology (gold standard). However, the sensitivity of this technique is low. Our goal was to evaluate whether circulating tumor cell (CTC) detection in CSF using the CellSearch® system could be used for LM diagnosis. Methods This
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Differences between Educational and Regulatory External Quality Assurance/Proficiency Testing Schemes. Clin. Chem. (IF 12.167) Pub Date : 2022-08-12 Tony Badrick,Graham Jones,W Greg Miller,Mauro Panteghini,Andrew Quintenz,Sverre Sandberg,Michael Spannagl
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An Update on Laboratory-Based Diagnostic Biomarkers for Multiple Sclerosis and Beyond. Clin. Chem. (IF 12.167) Pub Date : 2022-08-08 Ruba S Saadeh,Paola A Ramos,Alicia Algeciras-Schimnich,Eoin P Flanagan,Sean J Pittock,Maria Alice Willrich
BACKGROUND Multiple sclerosis (MS) is an immune-mediated central nervous system (CNS) inflammatory demyelinating disease in which analysis of clinical presentation, imaging studies, and laboratory tests aid in diagnosis. CONTENT This review discusses laboratory tests ordered to rule out and rule in MS, such as the traditional measurement of cerebrospinal fluid (CSF) IgG index and oligoclonal bands
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Assays Specific for BNP1-32 and NT-proBNP Exhibit a Similar Performance to Two Widely Used Assays in the Diagnosis of Heart Failure Clin. Chem. (IF 12.167) Pub Date : 2022-08-06 Lynley K Lewis, Sara D Raudsepp, Joanna C Whitlow, Sarah Appleby, Christopher J Pemberton, Timothy G Yandle, A Mark Richards
Background Secretion of cardioprotective B-type natriuretic peptide 1–32 (BNP1-32) is increased proportionately with cardiac dysfunction, but its measurement in plasma is difficult. Therefore, less specific BNP and amino-terminal proBNP (NT-proBNP) assays that detect the precursor molecule proBNP alongside BNP or NT-proBNP metabolites were developed to reflect BNP1-32 secretion and are now mandated
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Cell-Free RNA Transcriptome and Prediction of Adverse Pregnancy Outcomes. Clin. Chem. (IF 12.167) Pub Date : 2022-08-05 Kathryn J Gray,Martin Hemberg,S Ananth Karumanchi
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Effect of Macrotroponin in a Cohort of Community Patients with Elevated Cardiac Troponin Clin. Chem. (IF 12.167) Pub Date : 2022-08-05 Leo Lam, Rexson Tse, Patrick Gladding, Campbell Kyle
Background Macrotroponin is an important cause of discrepancy between current high-sensitivity cardiac troponin (hs-cTn) assays, however, its clinical significance is unclear. This study examined the effects of macrotroponin and repeat testing by different hs-cTnI assays in a cohort of community patients with elevated hs-cTnI. Methods The first residual serum specimen from each patient in the community
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A Targeted Liquid Chromatography–Tandem Mass Spectrometry Method for Simultaneous Quantification of Peptides from the Carboxyl-terminal Region of Type III Procollagen, Biomarkers of Collagen Turnover Clin. Chem. (IF 12.167) Pub Date : 2022-07-30 Huu-Hien Huynh, Katrina Forrest, Jessica O Becker, Michelle A Emrick, Geoffrey D Miller, Danielle Moncrieffe, David A Cowan, Andreas Thomas, Mario Thevis, Michael J MacCoss, Ben Hoffstrom, Peter H Byers, Daniel Eichner, Andrew N Hoofnagle
Background The development of analytical approaches to help reduce the risk of growth hormone (GH) doping is important to fair competition and the health of athletes. However, the reliable detection of GH use remains challenging. The identification of novel biomarkers of GH administration could lead to a better understanding of the physiological response to GH, more sensitive detection of the illicit
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Diabetes Duration and Subclinical Myocardial Injury: The Atherosclerosis Risk in Communities Study (ARIC) Clin. Chem. (IF 12.167) Pub Date : 2022-07-29 Carine E Hamo, Justin B Echouffo-Tcheugui, Sui Zhang, Roberta Florido, James S Pankow, Erin D Michos, Ronald Goldberg, Vijay Nambi, Gary Gerstenblith, Wendy S Post, Roger S Blumenthal, Christie Ballantyne, Elizabeth Selvin, Josef Coresh, Chiadi E Ndumele
Background Diabetes exerts adverse effects on the heart, and a longer diabetes duration is associated with greater heart failure risk. We studied diabetes duration and subclinical myocardial injury, as reflected by high-sensitivity cardiac troponin (hs-cTnT). Methods We analyzed 9052 participants without heart failure or coronary heart disease (mean age 63 years, 58% female, 21% Black, 15% with diabetes)
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Assessment of LDL-C Calculation Using the Newly Adopted NIH LDL-C Equation in a Pediatric Population. Clin. Chem. (IF 12.167) Pub Date : 2022-07-28 Ka Keung Chan,Jane A Dickerson
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Cardiac Troponin as a Marker of Heart Failure Risk in Diabetes. Clin. Chem. (IF 12.167) Pub Date : 2022-07-28 Eric S Kilpatrick
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Estimated Atherosclerotic Cardiovascular Disease Risk Score: An Automated Decision Aid for Statin Therapy Clin. Chem. (IF 12.167) Pub Date : 2022-07-28 Maureen Sampson, Anna Wolska, Marcelo Amar, Masako Ueda, Richard Dunbar, Daniel Soffer, Alan T Remaley
Background Estimation of atherosclerotic cardiovascular disease (ASCVD) risk is a key step in cardiovascular disease (CVD) prevention, but it requires entering additional risk factor information into a computer. We developed a simplified ASCVD risk score that can be automatically calculated by the clinical laboratory when a fasting standard lipid panel is reported. Methods Equations for an estimated
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Macrotroponin Complex as a Cause for Cardiac Troponin Increase after COVID-19 Vaccination and Infection. Clin. Chem. (IF 12.167) Pub Date : 2022-07-27 Anda Bularga,Ellen Oskoui,Takeshi Fujisawa,Sara Jenks,Rachel Sutherland,Fred S Apple,Ola Hammarsten,Nicholas L Mills
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Commentary on Macrotroponin Complex as a Cause for Cardiac Troponin Increase after COVID-19 Vaccination and Infection. Clin. Chem. (IF 12.167) Pub Date : 2022-07-27 Peter A Kavsak
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Commentary on Macrotroponin Complex as a Cause for Cardiac Troponin Increase after COVID-19 Vaccination and Infection. Clin. Chem. (IF 12.167) Pub Date : 2022-07-27 Bernard Croal
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Commentary on Questionable High Free T4 Concentrations: When Confirming against an Alternative Method Is Not Enough. Clin. Chem. (IF 12.167) Pub Date : 2022-07-23 Robert D Nerenz
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Clinical Validation of Genome Reference Consortium Human Build 38 in a Laboratory Utilizing Next-Generation Sequencing Technologies Clin. Chem. (IF 12.167) Pub Date : 2022-07-23 Lisa A Lansdon, Maxime Cadieux-Dion, John C Herriges, Jeffrey Johnston, Byunggil Yoo, Joseph T Alaimo, Isabelle Thiffault, Neil Miller, Ana S A Cohen, Elena A Repnikova, Lei Zhang, Midhat S Farooqi, Emily G Farrow, Carol J Saunders
Background Laboratories utilizing next-generation sequencing align sequence data to a standardized human reference genome (HRG). Several updated versions, or builds, have been released since the original HRG in 2001, including the Genome Reference Consortium Human Build 38 (GRCh38) in 2013. However, most clinical laboratories still use GRCh37, which was released in 2009. We report our laboratory’s
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Recovery Efficiency of Cell-Free DNA After Bisulfite Conversion. Clin. Chem. (IF 12.167) Pub Date : 2022-07-20 Teagan Fisher,Caroline E Ford,Kristina Warton
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Can Rapid Nanopore Sequencing Bring Genomic Testing to the Bedside? Clin. Chem. (IF 12.167) Pub Date : 2022-07-15 Sebastian Lunke,Zornitza Stark
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Estimating Analytical Errors of Glomerular Filtration Rate Measurement Clin. Chem. (IF 12.167) Pub Date : 2022-07-08 Damiano Ognissanti, Moa Andresen Bergström, Elvar Theodorsson, Anders Larsson, Gunnar Nordin, Ola Hammarsten
Background Few studies are available on how to optimize time points for sampling and how to estimate effects of analytical uncertainty when glomerular filtration rate (GFR) is calculated. Methods We explored the underlying regression mathematics of how analytical variation of a kidney filtration marker affects 1-compartment, slope-and-intercept GFR calculations, using 2 or 3 time points following a
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Harmonization Status of Serum Ferritin Measurements and Implications for Use as Marker of Iron-Related Disorders Clin. Chem. (IF 12.167) Pub Date : 2022-07-07 Federica Braga, Sara Pasqualetti, Erika Frusciante, Francesca Borrillo, Mariia Chibireva, Mauro Panteghini
Background Serum ferritin is considered a suitable biomarker of iron-related disorders. However, data about the comparability of results among commercial measuring systems (MSs) are contradictory. We performed an intercomparison study aimed at verifying the current interassay variability and its impact on clinical application of the test. Obtaining this information is vital because manufacturers continue
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A 6-Month-Old Infant with Severe Failure to Thrive during COVID-19 Pandemic. Clin. Chem. (IF 12.167) Pub Date : 2022-07-03 Xinying Hong,Hana Alharbi,Daniah Albokhari,Andrew C Edmondson,Miao He
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Development of a Rapid Assay for On-Site THC Detection in Oral Fluid. Clin. Chem. (IF 12.167) Pub Date : 2022-07-03 Kang Xiong-Hang,Sara A Love
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Clinicians at Crossroads for a Dangerous Interference in Neonatal Bilirubin Determination at the Point-of-Care. Clin. Chem. (IF 12.167) Pub Date : 2022-07-03 Giulia Colombo,Dominika Szoke,Elena Aloisio,Francesco Cavigioli,Alberto Dolci,Mauro Panteghini
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Commentary on Clinicians at Crossroads for a Dangerous Interference in Neonatal Bilirubin Determination at the Point-of-Care. Clin. Chem. (IF 12.167) Pub Date : 2022-07-03 Stanley Lo
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Cell-Free DNA as Biomarker for Sepsis by Integration of Microbial and Host Information Clin. Chem. (IF 12.167) Pub Date : 2022-06-30 Qiuyu Jing, Chi Hung Czarina Leung, Angela Ruohao Wu
Background Cell-free DNA (cfDNA) is emerging as a biomarker for sepsis. Previous studies have been focused mainly on identifying blood infections or simply quantifying cfDNA. We propose that by characterizing multifaceted unexplored components, cfDNA could be more informative for assessing this complex syndrome. Methods We explored multiple aspects of cfDNA in septic and nonseptic intensive care unit
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Pan-Cancer Detection and Typing by Mining Patterns in Large Genome-Wide Cell-Free DNA Sequencing Datasets Clin. Chem. (IF 12.167) Pub Date : 2022-06-30 Huiwen Che, Tatjana Jatsenko, Liesbeth Lenaerts, Luc Dehaspe, Leen Vancoillie, Nathalie Brison, Ilse Parijs, Kris Van Den Bogaert, Daniela Fischerova, Ruben Heremans, Chiara Landolfo, Antonia Carla Testa, Adriaan Vanderstichele, Lore Liekens, Valentina Pomella, Agnieszka Wozniak, Christophe Dooms, Els Wauters, Sigrid Hatse, Kevin Punie, Patrick Neven, Hans Wildiers, Sabine Tejpar, Diether Lambrechts
Background Cell-free DNA (cfDNA) analysis holds great promise for non-invasive cancer screening, diagnosis, and monitoring. We hypothesized that mining the patterns of cfDNA shallow whole-genome sequencing datasets from patients with cancer could improve cancer detection. Methods By applying unsupervised clustering and supervised machine learning on large cfDNA shallow whole-genome sequencing datasets
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Growth Differentiation Factor 15 and the Subsequent Risk of Atrial Fibrillation: The Atherosclerosis Risk in Communities Study Clin. Chem. (IF 12.167) Pub Date : 2022-06-28 Mengkun Chen, Ning Ding, Yejin Mok, Lena Mathews, Ron C Hoogeveen, Christie M Ballantyne, Lin Yee Chen, Josef Coresh, Kunihiro Matsushita
Background Growth differentiation factor 15 (GDF-15) is a stress-responsive biomarker associated with several types of cardiovascular diseases. However, conflicting results have been reported regarding its association with incident atrial fibrillation (AF) in the general population. Methods In 10 234 White and Black Atherosclerosis Risk in Communities (ARIC) Study participants (mean age 60 years, 20
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First Trimester Noninvasive Prenatal Diagnosis of Maternally Inherited Beta-Thalassemia Mutations. Clin. Chem. (IF 12.167) Pub Date : 2022-07-27 Tracey E Madgett
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The Curious Case of Synergy between Lipoprotein (a), Coronary Calcification, and Cardiovascular Disease Risk. Clin. Chem. (IF 12.167) Pub Date : 2022-06-18 Zaid I Almarzooq,Samia Mora
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Recent Developments in Mutation Enrichment and Detection Technologies Clin. Chem. (IF 12.167) Pub Date : 2022-06-18 Farzaneh Darbeheshti, Fangyan Yu, Farzana Ahmed, Viktor A Adalsteinsson, G Mike Makrigiorgos
Background Presence of excess unaltered, wild-type DNA (wtDNA) providing information of little clinical value may often mask low-level mutations containing important diagnostic or therapeutic clues. This is a recurring hurdle in biotechnology and medicine, including cancer, prenatal diagnosis, infectious diseases, and organ transplantation. Mutation enrichment techniques that allow reduction of unwanted
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Analytical Considerations in Deriving 99th Percentile Upper Reference Limits for High-Sensitivity Cardiac Troponin Assays: Educational Recommendations from the IFCC Committee on Clinical Application of Cardiac Bio-Markers Clin. Chem. (IF 12.167) Pub Date : 2022-06-18 Kristin M Aakre, Amy K Saenger, Rick Body, Paul Collinson, Ola Hammarsten, Allan S Jaffe, Pete Kavsak, Torbjørn Omland, Jordi Ordonez-Lianos, Fred S Apple
The International Federation of Clinical Chemistry Committee on Clinical Application of Cardiac Bio-Markers provides evidence-based educational documents to facilitate uniform interpretation and utilization of cardiac biomarkers in clinical laboratories and practice. The committee’s goals are to improve the understanding of certain key analytical and clinical aspects of cardiac biomarkers and how these
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Single-Cell Identification, Drug Susceptibility Test, and Whole-genome Sequencing of Helicobacter pylori Directly from Gastric Biopsy by Clinical Antimicrobial Susceptibility Test Ramanometry. Clin. Chem. (IF 12.167) Pub Date : 2022-07-27 Min Liu,Pengfei Zhu,Lei Zhang,Yanhai Gong,Chen Wang,Lu Sun,Lili Wang,Rongze Chen,Yuli Mao,Xiaoting Fu,Lili Zhang,Teng Xu,Yuetong Ji,Quanjiang Dong,Bo Ma,Jianzhong Zhang,Jian Xu
BACKGROUND The battle against Helicobacter pylori (H. pylori) infections demands fast, reliable, and sensitive methods for pathogen identification (ID), antimicrobial susceptibility tests (ASTs) based on metabolic response, and genome-wide mutation profiling that reveals resistance mechanisms. METHODS Here we introduce Clinical Antimicrobial Susceptibility Test Ramanometry for H. pylori (CAST-R-HP)
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Clinical Metagenomics-from Proof-of-Concept to Routine Use. Clin. Chem. (IF 12.167) Pub Date : 2022-07-27 Robert Schlaberg
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Comprehensive Analysis of Congenital Adrenal Hyperplasia Using Long-Read Sequencing. Clin. Chem. (IF 12.167) Pub Date : 2022-07-03 Yingdi Liu,Miaomiao Chen,Jing Liu,Aiping Mao,Yanling Teng,Huiming Yan,Huimin Zhu,Zhuo Li,Desheng Liang,Lingqian Wu
BACKGROUND Congenital adrenal hyperplasia (CAH) is an autosomal recessive disorder that has been included in newborn screening programs. Current approaches to gene testing for CAH are facing challenges because of the complexity of the CYP21A2 locus and genetic heterogeneity of the disease. METHODS A comprehensive analysis of CAH (CACAH) combining long-range locus-specific PCR and long-read sequencing
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A Practical Guide to Bayesian Statistics in Laboratory Medicine. Clin. Chem. (IF 12.167) Pub Date : 2022-07-03 Edmund H Wilkes
Statistical analyses form a fundamental part of causal inference in the experimental sciences. The statistical paradigm most commonly taught to science students around the world is that of frequentism, with a particular emphasis on the null hypothesis significance testing borne by the work of Neyman and Pearson in the early 20th century. This paradigm is often lauded as being the most objective of
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A Lipid Signature with Perturbed Triacylglycerol Co-Regulation, Identified from Targeted Lipidomics, Predicts Risk for Type 2 Diabetes and Mediates the Risk from Adiposity in Two Prospective Cohorts of Chinese Adults Clin. Chem. (IF 12.167) Pub Date : 2022-06-16 Gaokun Qiu, Hao Wang, Qi Yan, Hongxia Ma, Rundong Niu, Yanshou Lei, Yang Xiao, Lue Zhou, Handong Yang, Chengwei Xu, Xiaomin Zhang, Meian He, Huiru Tang, Zhibin Hu, An Pan, Hongbing Shen, Tangchun Wu
Background The roles of individual and co-regulated lipid molecular species in the development of type 2 diabetes (T2D) and mediation from metabolic risk factors remain unknown. Methods We conducted profiling of 166 plasma lipid species in 2 nested case-control studies within 2 independent cohorts of Chinese adults, the Dongfeng-Tongji and the Jiangsu non-communicable disease cohorts. After 4.61 (0
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Diagnostic Performance of Metagenomic Next-Generation Sequencing in Pediatric Patients: A Retrospective Study in a Large Children's Medical Center. Clin. Chem. (IF 12.167) Pub Date : 2022-07-27 Yue Tao,Hui Yan,Yujie Liu,Fang Zhang,Lijuan Luo,Yajuan Zhou,Kang An,Ruwen Yang,Bin Yang,Teng Xu,Li Xie,Hong Ren,Zhuoming Xu,Qing Cao,Xi Mo
BACKGROUND Metagenomic next-generation sequencing (mNGS) has the potential to become a complementary, if not essential, test in some clinical settings. However, the clinical application of mNGS in a large population of children with various types of infectious diseases (IDs) has not been previously evaluated. METHODS From April 2019 to April 2021, 640 samples were collected at a single pediatric hospital
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The Need to Develop Clinical Guidance for the Use of High-Sensitivity Cardiac Troponin in Pediatric and Neonatal Patients. Clin. Chem. (IF 12.167) Pub Date : 2022-07-03 Alan Wang,Yader Sandoval,Fred S Apple,James Homme,Allan S Jaffe,
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Improving Harmonization and Standardization of Expanded Newborn Screening Results by Optimization of the Legacy Flow Injection Analysis Tandem Mass Spectrometry Methods and Application of a Standardized Calibration Approach. Clin. Chem. (IF 12.167) Pub Date : 2022-07-27 Rachel S Carling,Emily Whyte,Catharine John,Rachelle Garstone,Philippa Goddard,Toby Greenfield,Sarah L Hogg,Clare Le Masurier,Simon Cowen,Stuart J Moat,Christopher Hopley
BACKGROUND Newborn screening (NBS) laboratories in the United Kingdom adhere to common protocols based on single analyte cutoff values (COVs); therefore, interlaboratory harmonization is of paramount importance. Interlaboratory variation for screening analytes in UK NBS laboratories ranges from 17% to 59%. While using common stable isotope internal standards has been shown to significantly reduce interlaboratory
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Continual Improvement of the Reliability of Next-Generation Sequencing-Based ctDNA Analysis: A Long-Term Comparison of ctDNA Detection in China. Clin. Chem. (IF 12.167) Pub Date : 2022-07-03 Rongxue Peng,Rui Zhang,Jinming Li
BACKGROUND Since circulating tumor DNA (ctDNA) sequencing is increasingly being applied in clinical management of patients with cancer, its testing accuracy has become a matter of serious concern. To address this issue, a long-term ctDNA analysis proficiency testing (PT) scheme for next-generation sequencing (NGS) was launched in China in 2018, serving as an educational tool for assessing and improving
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Public Misinformation and Science Communication in Times of Public Health Crises. Clin. Chem. (IF 12.167) Pub Date : 2022-07-27 Michael A Vera,Joe M El-Khoury,Holden Thorp,Richard J Tofel,Joseph S Ross,Apoorva Mandavilli,Eric Topol
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PCR-Based Screening Tests for SARS-CoV-2 Mutations: What Is the Best Way to Identify Variants? Clin. Chem. (IF 12.167) Pub Date : 2022-07-27 Akira Aoki,Yoko Mori,Yoshinori Okamoto,Hideto Jinno
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Optimized Droplet Digital PCR Assay on Cell-Free DNA Samples for Non-Invasive Prenatal Diagnosis: Application to Beta-Thalassemia. Clin. Chem. (IF 12.167) Pub Date : 2022-07-27 Constantina G Constantinou,Eleni Karitzi,Stefania Byrou,Coralea Stephanou,Kyriaki Michailidou,Christiana Makariou,Georgia Hadjilambi,Agathoklis Christofides,Marina Kleanthous,Thessalia Papasavva
BACKGROUND Thalassemias are inherited blood disorders and by far one of the most common monogenic diseases globally. Beta-thalassemia has a particularly high prevalence in Cyprus, with the IVSI-110 G>A (HBB:c.93-21G>A) pathogenic variation representing almost 79% of the total carriers. The discovery that 3% to 20% of cell-free fetal DNA (cffDNA) is present in the maternal plasma allowed the development
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Expression Patterns and Corepressor Function of Retinoic Acid-induced 2 in Prostate Cancer. Clin. Chem. (IF 12.167) Pub Date : 2022-07-03 Katharina Besler,Aleksandra Węglarz,Laura Keller,Gunhild von Amsberg,Natalia Bednarz-Knoll,Anne Offermann,Sara Stoupiec,Elke Eltze,Axel Semjonow,Lena Boettcher,Svenja Schneegans,Sven Perner,Siegfried Hauch,Tilman Todenhöfer,Sven Peine,Klaus Pantel,Harriet Wikman,Stefan Werner
BACKGROUND Revealing molecular mechanisms linked to androgen receptor activity can help to improve diagnosis and treatment of prostate cancer. Retinoic acid-induced 2 (RAI2) protein is thought to act as a transcriptional coregulator involved in hormonal responses and epithelial differentiation. We evaluated the clinical relevance and biological function of the RAI2 protein in prostate cancer. METHODS
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Rh Blood Group D Antigen Genotyping Using a Portable Nanopore-based Sequencing Device: Proof of Principle. Clin. Chem. (IF 12.167) Pub Date : 2022-06-02 Wajnat A Tounsi,Vasileios P Lenis,Silja M Tammi,Susanna Sainio,Katri Haimila,Neil D Avent,Tracey E Madgett
BACKGROUND Nanopore sequencing is direct sequencing of a single-stranded DNA molecule using biological pores. A portable nanopore-based sequencing device from Oxford Nanopore Technologies (MinION) depends on driving a DNA molecule through nanopores embedded in a membrane using a voltage. Changes in current are then measured by a sensor, thousands of times per second and translated to nucleobases. METHODS
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Commentary on Perplexingly High Tacrolimus Concentrations in a Renal Transplant Patient with HIV. Clin. Chem. (IF 12.167) Pub Date : 2022-06-01 Nicholas E Heger
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Perplexingly High Tacrolimus Concentrations in a Renal Transplant Patient with HIV. Clin. Chem. (IF 12.167) Pub Date : 2022-06-01 Victoria Higgins,Bhushan M Kapur,Daniel R Beriault,Sarah R Delaney
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Commentary on Perplexingly High Tacrolimus Concentrations in a Renal Transplant Patient with HIV. Clin. Chem. (IF 12.167) Pub Date : 2022-06-01 Nitender Goyal
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Pain, Pain, Go Away: Exploring the Role of the Immune System in Regulating Chronic Pain. Clin. Chem. (IF 12.167) Pub Date : 2022-06-01 Kai J Rogers,Anna E Merrill
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Are Anti-Receptor-Binding Domain Antibodies Still a Relevant Proxy for Monitoring SARS-CoV-2 Neutralizing Activity in the Omicron Era? Clin. Chem. (IF 12.167) Pub Date : 2022-07-03 Kahina Saker,Bruno Pozzetto,Antonin Bal,Martine Valette,Jean Baptiste Fassier,Carla Saade,Mary Anne Trabaud,Sophie Trouillet-Assant,
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Multiplex Fragment Analysis for Flexible Detection of All SARS-CoV-2 Variants of Concern. Clin. Chem. (IF 12.167) Pub Date : 2022-07-27 Andrew E Clark,Zhaohui Wang,Emily Ostman,Hui Zheng,Huiyu Yao,Brandi Cantarel,Mohammed Kanchwala,Chao Xing,Li Chen,Pei Irwin,Yan Xu,Dwight Oliver,Francesca M Lee,Jeffrey R Gagan,Laura Filkins,Alagarraju Muthukumar,Jason Y Park,Ravi Sarode,Jeffrey A SoRelle
BACKGROUND Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants continue to emerge, and effective tracking requires rapid return of results. Surveillance of variants is typically performed by whole genome sequencing (WGS), which can be financially prohibitive and requires specialized equipment and bioinformatic expertise. Genotyping approaches are rapid methods for monitoring SARS-CoV-2
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Critical Roles of Clinical Laboratorians in Translational Research and Applied Sciences-Experts' Insights and Experiences. Clin. Chem. (IF 12.167) Pub Date : 2022-07-03 Moderators Christopher Farnsworth,Zhen Zhao,Experts Fred Apple,Paul Collinson,Mari L DeMarco,Ann Gronowski,David B Sacks,Yusheng Zhu
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Dutch National Round Robin Trial on Plasma-Derived Circulating Cell-Free DNA Extraction Methods Routinely Used in Clinical Pathology for Molecular Tumor Profiling. Clin. Chem. (IF 12.167) Pub Date : 2022-07-03 Paul van der Leest,Emma M Ketelaar,Carel J M van Noesel,Daan van den Broek,Robert A A van Boerdonk,Birgit Deiman,Naomi Rifaela,Robert van der Geize,Cornelis J J Huijsmans,Ernst Jan M Speel,Maartje J Geerlings,Ron H N van Schaik,Maurice P H M Jansen,Ria Dane-Vogelaar,Else Driehuis,Mathie P G Leers,Grigory Sidorenkov,Menno Tamminga,Léon C van Kempen,Ed Schuuring
BACKGROUND Efficient recovery of circulating tumor DNA (ctDNA) depends on the quantity and quality of circulating cell-free DNA (ccfDNA). Here, we evaluated whether various ccfDNA extraction methods routinely applied in Dutch laboratories affect ccfDNA yield, ccfDNA integrity, and mutant ctDNA detection, using identical lung cancer patient-derived plasma samples. METHODS Aliquots of 4 high-volume diagnostic
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Toward a Personal Drug Detection Assistant: Machine Learning for Detection of Synthetic Cannabinoid Receptor Agonists. Clin. Chem. (IF 12.167) Pub Date : 2022-07-03 Shannon Haymond
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Women in Laboratory Medicine: A Q&A on Diversity and Inclusion. Clin. Chem. (IF 12.167) Pub Date : 2022-06-01 Nadia Ayala-Lopez,Octavia M Peck Palmer,M Laura Parnas,Lakshmi V Ramanathan,Zahra Shajani-Yi
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Repeat Expansion Disorders: Bridging the Gap. Clin. Chem. (IF 12.167) Pub Date : 2022-06-01 Edwin P Kirk
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Partial Postponement of the Application of the In Vitro Diagnostic Medical Devices Regulation in the European Union. Clin. Chem. (IF 12.167) Pub Date : 2022-06-01 Michael Vogeser,Monika Brüggemann,Jochen Lennerz,Albrecht Stenzinger,Ulrich M Gassner
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Jagged Ends on Multinucleosomal Cell-Free DNA Serve as a Biomarker for Nuclease Activity and Systemic Lupus Erythematosus. Clin. Chem. (IF 12.167) Pub Date : 2022-07-03 Spencer C Ding,Rebecca W Y Chan,Wenlei Peng,Liangbo Huang,Ze Zhou,Xi Hu,Stefano Volpi,Linda T Hiraki,Augusto Vaglio,Paride Fenaroli,Paola Bocca,Lai Shan Tam,Priscilla C H Wong,Lydia H P Tam,Peiyong Jiang,Rossa W K Chiu,K C Allen Chan,Y M Dennis Lo
BACKGROUND Jagged ends of plasma DNA are a recently recognized class of fragmentomic markers for cell-free DNA, reflecting the activity of nucleases. A number of recent studies have also highlighted the importance of jagged ends in the context of pregnancy and oncology. However, knowledge regarding the generation of jagged ends is incomplete. METHODS Jaggedness of plasma DNA was analyzed based on Jag-seq