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Salmonella utilizes L-arabinose to silence virulence gene expression for accelerated pathogen growth within the host. Gut Microbes (IF 12.2) Pub Date : 2025-02-15 Jingchen Yu,Huang Tang,Yana Chen,Zuoqiang Wang,Wanqiu Huang,Tao Zhou,Bingjie Wen,Chengyue Wang,Shuang Gu,Jinjing Ni,Jing Tao,Danni Wang,Jie Lu,Qing Xie,Yu-Feng Yao
Carbon source is an important nutrient for bacteria to sustain growth and often acts as a signal that modulates virulence expression. L-arabinose is produced by plants and plays an important role in regulating the global gene expression of bacteria. Previously, we have shown that L-arabinose induces a more severe systemic infection in Salmonella-infected mice with normal microbiota, but does not affect
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Time-restricted feeding promotes glucagon-like peptide-1 secretion and regulates appetite via tryptophan metabolism of gut Lactobacillus in pigs. Gut Microbes (IF 12.2) Pub Date : 2025-02-14 Qiuke Li,Ding Tan,Shijie Xiong,Kaifan Yu,Yong Su,Weiyun Zhu
Previous clinical trials have shown that time-restricted feeding can be involved in regulating the metabolic health of humans and animals. However, the underlying mechanism has not been fully explored. In this study, the pig model was employed to simulate four prevalent human eating habits, with the aim of investigating the impact of gut microbiota and microbial metabolites on gut hormone secretion
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Probiotic supplementation mitigates sex-dependent nociceptive changes and gut dysbiosis induced by prenatal opioid exposure. Gut Microbes (IF 12.2) Pub Date : 2025-02-14 Salma Singh,Yaa Abu,Danielle Antoine,Daniel Gomez,Junyi Tao,Bridget Truitt,Sabita Roy
The gut microbiome has emerged as a promising target for modulating adverse effects of opioid exposure due to its significant role in health and disease. Opioid use disorder (OUD) has become increasingly prevalent, specifically in women of reproductive age, contributing to an increased incidence of offspring exposed to opioids in utero. Recent studies have shown that prenatal opioid exposure (POE)
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IgG-seq identifies immune-reactive enteric bacteria in Crohn's disease with spondyloarthritis. Gut Microbes (IF 12.2) Pub Date : 2025-02-13 Grace A Maldarelli,Maeva Metz,Seun Oguntunmibi,Nancy Tran,Grace Xiang,Dana Lukin,Ellen J Scherl,Randy S Longman
Joint inflammation is the most common extraintestinal manifestation of Crohn's disease (CD). Although alterations in the enteric microbiota are described in CD with spondyloarthritis (CD-SpA), it is not known whether distinct taxa serve as markers for clinical subtypes of axial (AxSpA) or peripheral SpA (pSpA) in CD. Moreover, it is not yet known whether these taxa generate a specific systemic IgG
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Long-term yogurt intake and colorectal cancer incidence subclassified by Bifidobacterium abundance in tumor. Gut Microbes (IF 12.2) Pub Date : 2025-02-12 Satoko Ugai,Li Liu,Keisuke Kosumi,Hidetaka Kawamura,Tsuyoshi Hamada,Kosuke Mima,Kota Arima,Kazuo Okadome,Qian Yao,Kosuke Matsuda,Yuxue Zhong,Hiroki Mizuno,Andrew T Chan,Wendy S Garrett,Mingyang Song,Marios Giannakis,Edward L Giovannucci,Xuehong Zhang,Shuji Ogino,Tomotaka Ugai
Evidence suggests a tumor-suppressive effect of the intake of yogurt, which typically contains Bifidobacterium. We hypothesized that long-term yogurt intake might be associated with colorectal cancer incidence differentially by tumor subgroups according to the amount of tissue Bifidobacterium. We utilized the prospective cohort incident-tumor biobank method and resources of two prospective cohort studies
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Maternal-infant probiotic transmission mitigates early-life stress-induced autism in mice. Gut Microbes (IF 12.2) Pub Date : 2025-02-11 Li Qing,Xin Qian,Huiyue Zhu,Jingyu Wang,Jingge Sun,Zhiying Jin,Xinyu Tang,Yingqi Zhao,Gang Wang,Jianxin Zhao,Wei Chen,Peijun Tian
Autism, a disorder influenced by both genetic and environmental factors, presents significant challenges for prevention and treatment. While maternal-infant gut microbiota has been a focus in autism research, preventive strategies targeting maternal gut microbiota remain underexplored. This study demonstrates that prenatal probiotic intake can effectively prevent maternal separation-induced autistic-like
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Helicobacter pylori infection induces DNA double-strand breaks through the ACVR1/IRF3/POLD1 signaling axis to drive gastric tumorigenesis. Gut Microbes (IF 12.2) Pub Date : 2025-02-09 Xinbo Xu,Xiao Fei,Huan Wang,Xidong Wu,Yuan Zhan,Xin Li,Yan'an Zhou,Chunxi Shu,Cong He,Yi Hu,Jianping Liu,Nonghua Lv,Nianshuang Li,Yin Zhu
Helicobacter pylori (H. pylori) infection plays a pivotal role in gastric carcinogenesis through inflammation-related mechanisms. Activin A receptor type I (ACVR1), known for encoding the type I receptor for bone morphogenetic proteins (BMPs), has been identified as a cancer diver gene across various tumors. However, the specific role of AVCR1 in H. pylori-induced gastric tumorigenesis remains incompletely
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Bifidobacterium inhibits the progression of colorectal tumorigenesis in mice through fatty acid isomerization and gut microbiota modulation. Gut Microbes (IF 12.2) Pub Date : 2025-02-09 Yang Chen,Huiting Fang,Haiqin Chen,Xiaoming Liu,Jianxin Zhao,Catherine Stanton,R Paul Ross,Wei Chen,Bo Yang
Colorectal cancer (CRC) represents the third most common cancer worldwide. Consequently, there is an urgent need to identify novel preventive and therapeutic strategies for CRC. This study aimed to screen for beneficial bacteria that have a preventive effect on CRC and to elucidate the potential mechanisms. Initially, we compared gut bacteria and bacterial metabolites of healthy volunteers and CRC
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Uncovering de novo polyamine biosynthesis in the gut microbiome and its alteration in inflammatory bowel disease. Gut Microbes (IF 12.2) Pub Date : 2025-02-09 Xinwei Li,Xia Xiao,Shengnan Wang,Biyu Wu,Yixuan Zhou,Pan Deng
Polyamines are important gut microbial metabolites known to affect host physiology, yet the mechanisms behind their microbial production remain incompletely understood. In this study, we developed a stable isotope-resolved metabolomic (SIRM) approach to track polyamine biosynthesis in the gut microbiome. Viable microbial cells were extracted from fresh human and mouse feces and incubated anaerobically
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Alterations in mucosa branched N-glycans lead to dysbiosis and downregulation of ILC3: a key driver of intestinal inflammation. Gut Microbes (IF 12.2) Pub Date : 2025-02-07 Cláudia S Rodrigues,Joana Gaifem,Márcia S Pereira,Maria Francisca Alves,Mariana Silva,Nuno Padrão,Bruno Cavadas,Catarina Moreira-Barbosa,Inês Alves,Ricardo Marcos-Pinto,Joana Torres,Aonghus Lavelle,Jean-Frederic Colombel,Harry Sokol,Salomé S Pinho
The perturbation of the symbiotic relationship between microbes and intestinal immune system contributes to gut inflammation and Inflammatory Bowel Disease (IBD) development. The host mucosa glycans (glycocalyx) creates a major biological interface between gut microorganisms and host immunity that remains ill-defined. Glycans are essential players in IBD immunopathogenesis, even years before disease
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Early life gut microbiome and its impact on childhood health and chronic conditions. Gut Microbes (IF 12.2) Pub Date : 2025-02-07 Harold Nunez,Pamela A Nieto,Ruben A Mars,Maryam Ghavami,Cheryl Sew Hoy,Kimberley Sukhum
The development of the gut microbiome is crucial to human health, particularly during the first three years of life. Given its role in immune development, disturbances in the establishment process of the gut microbiome may have long term consequences. This review summarizes evidence for these claims, highlighting compositional changes of the gut microbiome during this critical period of life as well
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Post sleeve gastrectomy-enriched gut commensal Clostridia promotes secondary bile acid increase and weight loss. Gut Microbes (IF 12.2) Pub Date : 2025-02-06 Shaoqian Zhao,Huibin Lin,Wen Li,Xiaoqiang Xu,Qihan Wu,Zhifeng Wang,Juan Shi,Yufei Chen,Lingxia Ye,Liuqing Xi,Lijia Chen,Mingyang Yuan,Junlei Su,Aibo Gao,Jiabin Jin,Xiayang Ying,Xiaolin Wang,Yaorui Ye,Yingkai Sun,Yifei Zhang,Xiaxing Deng,Baiyong Shen,Weiqiong Gu,Guang Ning,Weiqing Wang,Jie Hong,Jiqiu Wang,Ruixin Liu
The gut microbiome is altered after bariatric surgery and is associated with weight loss. However, the commensal bacteria involved and the underlying mechanism remain to be determined. We performed shotgun metagenomic sequencing in obese subjects before and longitudinally after sleeve gastrectomy (SG), and found a significant enrichment in microbial species in Clostridia and bile acid metabolizing
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Archaea methanogens are associated with cognitive performance through the shaping of gut microbiota, butyrate and histidine metabolism. Gut Microbes (IF 12.2) Pub Date : 2025-02-05 Andrea Fumagalli,Anna Castells-Nobau,Dakshat Trivedi,Josep Garre-Olmo,Josep Puig,Rafel Ramos,Lluís Ramió-Torrentà,Vicente Pérez-Brocal,Andrés Moya,Jonathan Swann,Elena Martin-Garcia,Rafael Maldonado,José Manuel Fernández-Real,Jordi Mayneris-Perxachs
The relationship between bacteria, cognitive function and obesity is well established, yet the role of archaeal species remains underexplored. We used shotgun metagenomics and neuropsychological tests to identify microbial species associated with cognition in a discovery cohort (IRONMET, n = 125). Interestingly, methanogen archaeas exhibited the strongest positive associations with cognition, particularly
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Biofilm-associated proteins: from the gut biofilms to neurodegeneration. Gut Microbes (IF 12.2) Pub Date : 2025-02-03 Jaione Valle
Human microbiota form a biofilm with substantial consequences for health and disease. Numerous studies have indicated that microbial communities produce functional amyloids as part of their biofilm extracellular scaffolds. The overlooked interplay between bacterial amyloids and the host may have detrimental consequences for the host, including neurodegeneration. This work gives an overview of the biofilm-associated
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AhR Activation Transcriptionally Induces Anti-Microbial Peptide Alpha-Defensin 1 Leading to Reversal of Gut Microbiota Dysbiosis and Colitis. Gut Microbes (IF 12.2) Pub Date : 2025-02-02 Manikandan Palrasu,Khadija Kakar,Amarnath Marudamuthu,Hamida Hamida,Shruthi Thada,Yin Zhong,Shanieka Staley,Philip Brandon Busbee,Jie Li,Monica Garcia-Buitrago,Mitzi Nagarkatti,Prakash Nagarkatti
Alpha-defensin 1 is a small antimicrobial peptide that acts as the first line of defense against pathogens. It is induced following microbial cues and inflammatory signals in neutrophils and Paneth cells in the small intestine, which suggests that it plays a role in microbial homeostasis in the gut. The gut microbial products also serve as ligands for the aryl hydrocarbon receptor (AhR), an environmental
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PMA1-containing extracellular vesicles of Candida albicans triggers immune responses and colitis progression. Gut Microbes (IF 12.2) Pub Date : 2025-01-31 Zhen Xu,Shuping Qiao,Zelin Wang,Chen Peng,Yayi Hou,Baorui Liu,Guochun Cao,Tingting Wang
Candida albicans (C. albicans) exhibits aberrant changes in patients with colitis, and it has been reported to dominate the colonic mucosal immune response. Here, we found that PMA1 expression was significantly increased in C. albicans from patients with IBD compared to that in healthy controls. A Crispr-Cas9-based fungal strain editing system was then used to knock out PMA1 expression in C. albicans
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Lachnospiraceae-bacterium alleviates ischemia-reperfusion injury in steatotic donor liver by inhibiting ferroptosis via the Foxo3-Alox15 signaling pathway. Gut Microbes (IF 12.2) Pub Date : 2025-01-30 Shenghe Deng,Huan Cao,Tongxi Li,Xueling Wang,Junpeng Meng,Teng Zeng,Di Zhang,Shuhua Zhang,Guoliang Wang,Ran Liu,Tianhao Zou,Mao Cai,Ren Lang,Di Lu,Jinyang Gu
Ischemia-reperfusion injury (IRI) is a major obstacle in liver transplantation, especially with steatotic donor livers. Dysbiosis of the gut microbiota has been implicated in modulating IRI, and Lachnospiraceae plays a pivotal role in regulating host inflammatory and immune responses, but its specific role in liver transplantation IRI remains unclear. This study explores whether Lachnospiraceae can
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The role of the early-life gut microbiome in childhood asthma. Gut Microbes (IF 12.2) Pub Date : 2025-01-30 Ulrika Boulund,Jonathan Thorsen,Urvish Trivedi,Kaare Tranæs,Jie Jiang,Shiraz A Shah,Jakob Stokholm
Asthma is a chronic disease affecting millions of children worldwide, and in severe cases requires hospitalization. The etiology of asthma is multifactorial, caused by both genetic and environmental factors. In recent years, the role of the early-life gut microbiome in relation to asthma has become apparent, supported by an increasing number of population studies, in vivo research, and intervention
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Fusobacterium nucleatum upregulates the immune inhibitory receptor PD-L1 in colorectal cancer cells via the activation of ALPK1. Gut Microbes (IF 12.2) Pub Date : 2025-01-29 Coco Duizer,Moniek Salomons,Merel van Gogh,Sanne Gräve,Freke A Schaafsma,Maaike J Stok,Merel Sijbranda,Raghuvandhanan Kumarasamy Sivasamy,Rob J L Willems,Marcel R de Zoete
Fusobacterium nucleatum is a Gram-negative oncobacterium that is associated with colorectal cancer. The molecular mechanisms utilized by F. nucleatum to promote colorectal tumor development have largely focused on adhesin-mediated binding to the tumor tissue and on the pro-inflammatory capacity of F. nucleatum. However, the exact manner in which F. nucleatum promotes inflammation in the tumor microenvironment
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Pharmacomicrobiomics: a new field contributing to optimizing drug therapy in Parkinson's disease. Gut Microbes (IF 12.2) Pub Date : 2025-01-28 Yi Zhang,Chengjun Mo,Penghui Ai,Xiaoqin He,Qin Xiao,Xiaodong Yang
Gut microbiota, which act as a determinant of pharmacokinetics, have long been overlooked. In recent years, a growing body of evidence indicates that the gut microbiota influence drug metabolism and efficacy. Conversely, drugs also exert a substantial influence on the function and composition of the gut microbiota. Pharmacomicrobiomics, an emerging field focusing on the interplay of drugs and gut microbiota
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Targeted modulation of intestinal barrier and mucosal immune-related microbiota attenuates IgA nephropathy progression. Gut Microbes (IF 12.2) Pub Date : 2025-01-28 Ran Zhang,Yuyan Tang,Xiangru Feng,Xiaoxuan Lu,Mengyao Zhao,Jiayang Jin,Xiaoguo Ji,Haidong He,Liming Zhao
IgA nephropathy (IgAN) is related to the balance of gut microbiota. However, it is unclear whether changes in the gut microbiota can cause IgAN or attenuate its progression. This study employed IgAN and human microbiota-associated (HMA)-IgAN models to investigate the impact of IgAN on gut microbiota alteration and the mechanisms by which gut microbiota might trigger IgAN. Furthermore, this study examined
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Gut microbiota modulation via fecal microbiota transplantation mitigates hyperoxaluria and calcium oxalate crystal depositions induced by high oxalate diet. Gut Microbes (IF 12.2) Pub Date : 2025-01-28 Lingyue An,Shujue Li,Zhenglin Chang,Min Lei,Zhican He,Peng Xu,Shike Zhang,Zheng Jiang,Muhammad Sarfaraz Iqbal,Xinyuan Sun,Hongxing Liu,Xiaolu Duan,Wenqi Wu
Hyperoxaluria, including primary and secondary hyperoxaluria, is a disorder characterized by increased urinary oxalate excretion and could lead to recurrent calcium oxalate kidney stones, nephrocalcinosis and eventually end stage renal disease. For secondary hyperoxaluria, high dietary oxalate (HDOx) or its precursors intake is a key reason. Recently, accumulated studies highlight the important role
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The neonatal gut microbiome in health and disease. Gut Microbes (IF 12.2) Pub Date : 2025-01-27 Geoffrey A Preidis
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Mucus-penetrating microbiota drive chronic low-grade intestinal inflammation and metabolic dysregulation. Gut Microbes (IF 12.2) Pub Date : 2025-01-26 Melissa C Kordahi,Noëmie Daniel,Andrew T Gewirtz,Benoit Chassaing
Metabolic syndrome is, in humans, associated with alterations in the composition and localization of the intestinal microbiota, including encroachment of bacteria within the colon's inner mucus layer. Possible promoters of these events include dietary emulsifiers, such as carboxymethylcellulose (CMC) and polysorbate-80 (P80), which, in mice, result in altered microbiota composition, encroachment, low-grade
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Long COVID and gut microbiome: insights into pathogenesis and therapeutics. Gut Microbes (IF 12.2) Pub Date : 2025-01-24 Raphaela I Lau,Qi Su,Siew C Ng
Post-acute coronavirus disease 2019 syndrome (PACS), following severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection or coronavirus disease 2019 (COVID-19), is typically characterized by long-term debilitating symptoms affecting multiple organs and systems. Unfortunately, there is currently a lack of effective treatment strategies. Altered gut microbiome has been proposed as one of
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Novel role of FTO in regulation of gut-brain communication via Desulfovibrio fairfieldensis-produced hydrogen sulfide under arsenic exposure. Gut Microbes (IF 12.2) Pub Date : 2025-01-24 Ruonan Chen,Xiaoqin Chai,Yunxiao Zhang,Tianxiu Zhou,Yinyin Xia,Xuejun Jiang,Bo Lv,Jun Zhang,Lixiao Zhou,Xin Tian,Ruonan Wang,Lejiao Mao,Feng Zhao,Hongyang Zhang,Jun Hu,Jingfu Qiu,Zhen Zou,Chengzhi Chen
Fat mass and obesity-associated protein (FTO) is the key demethylase that reverses the abnormally altered N6-methyladenosine (m6A) modification in eukaryotic cells under environmental pollutants exposure. Arsenic is an environmental metalloid and can cause severe symptoms in human mainly through drinking water. However, there is no specific treatment for its toxic effects due to the uncovered mechanisms
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Interkingdom signaling between gastrointestinal hormones and the gut microbiome. Gut Microbes (IF 12.2) Pub Date : 2025-01-24 Xinyu Zhao,Ye Qiu,Lanfan Liang,Xiangsheng Fu
The interplay between the gut microbiota and gastrointestinal hormones plays a pivotal role in the health of the host and the development of diseases. As a vital component of the intestinal microecosystem, the gut microbiota influences the synthesis and release of many gastrointestinal hormones through mechanisms such as modulating the intestinal environment, producing metabolites, impacting mucosal
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The gut microbiota predicts and time-restricted feeding delays experimental colitis. Gut Microbes (IF 12.2) Pub Date : 2025-01-22 Hannah K Ruple,Eva Haasis,Anna Bettenburg,Carina Maier,Carolin Fritz,Laura Schüle,Sarah Löcker,Yvonne Soltow,Lynn Schintgen,Nina S Schmidt,Celine Schneider,Axel Lorentz,W Florian Fricke
The etiology of inflammatory bowel disease (IBD) remains unclear, treatment options unsatisfactory and disease development difficult to predict for individual patients. Dysbiosis of the gastrointestinal microbiota and disruption of the biological clock have been implicated and studied as diagnostic and therapeutic targets. Here, we examine the relationship of IBD to biological clock and gut microbiota
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Bacterial extracellular vesicles in the initiation, progression and treatment of atherosclerosis. Gut Microbes (IF 12.2) Pub Date : 2025-01-22 Yuling Lin,Jingyu Wang,Fan Bu,Ruyi Zhang,Junhui Wang,Yubing Wang,Mei Huang,Yiyi Huang,Lei Zheng,Qian Wang,Xiumei Hu
Atherosclerosis is the primary cause of cardiovascular and cerebrovascular diseases. However, current anti-atherosclerosis drugs have shown conflicting therapeutic outcomes, thereby spurring the search for novel and effective treatments. Recent research indicates the crucial involvement of oral and gastrointestinal microbiota in atherosclerosis. While gut microbiota metabolites, such as choline derivatives
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Stevioside mitigates metabolic dysregulation in offspring induced by maternal high-fat diet: the role of gut microbiota-driven thermogenesis. Gut Microbes (IF 12.2) Pub Date : 2025-01-21 Jin Ye,Renjie Shi,Xiaoning Wu,Hua Fan,Yapei Zhao,Xinyun Hu,Lulu Wang,Xiaowei Bo,Dongning Li,Yunshu Ge,Danna Wang,Bing Xia,Zhenting Zhao,Chunxia Xiao,Beita Zhao,Yutang Wang,Xuebo Liu
Maternal obesity poses a significant threat to the metabolic profiles of offspring. Microorganisms acquired from the mother early in life critically affect the host's metabolic functions. Natural non-nutritive sweeteners, particularly stevioside (STV), play a crucial role in reducing obesity and affecting gut microbiota composition. Based on this, we hypothesized that maternal STV supplementation could
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Comparing Campylobacter jejuni to three other enteric pathogens in OligoMM12 mice reveals pathogen-specific host and microbiota responses. Gut Microbes (IF 12.2) Pub Date : 2025-01-21 Mathias K-M Herzog,Audrey Peters,Nizar Shayya,Monica Cazzaniga,Kardokh Kaka Bra,Trisha Arora,Manja Barthel,Ersin Gül,Luca Maurer,Patrick Kiefer,Philipp Christen,Katharina Endhardt,Julia A Vorholt,Gad Frankel,Markus M Heimesaat,Stefan Bereswill,Cormac G M Gahan,Marcus J Claesson,Xavier Domingo-Almenara,Wolf-Dietrich Hardt
Campylobacter jejuni, non-typhoidal Salmonella spp., Listeria monocytogenes and enteropathogenic/enterohemorrhagic Escherichia coli (EPEC/EHEC) are leading causes of food-borne illness worldwide. Citrobacter rodentium has been used to model EPEC and EHEC infection in mice. The gut microbiome is well-known to affect gut colonization and host responses to many food-borne pathogens. Recent progress has
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Dysbiosis of infant gut microbiota is related to the altered fatty acid composition of human milk from mothers with gestational diabetes mellitus: a prospective cohort study. Gut Microbes (IF 12.2) Pub Date : 2025-01-21 Kelei Li,Jin Jin,Zhizuo Liu,Chuanjing Chen,Ludi Huang,Yongye Sun
Gestational diabetes mellitus (GDM) is known to be associated with dysbiosis of offspring gut microbiota, but the mechanism remains unclear. The present prospective study explored the role of human milk fatty acid composition in this association. Mothers with GDM and normal controls were recruited at 24-28 gestational weeks. Follow-up was conducted at 1-3 days postpartum and 1 month postpartum to collect
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Reinvestigation into the role of lipopolysaccharide Glycosyltransferases in Helicobacter pylori protein glycosylation. Gut Microbes (IF 12.2) Pub Date : 2025-01-20 Hong Li,Xiaoqiong Tang,Tiandi Yang,Tingting Liao,Aleksandra W Debowski,Tiankuo Yang,Yalin Shen,Hans-Olof Nilsson,Stuart M Haslam,Barbara Mulloy,Anne Dell,Keith A Stubbs,Wolfgang Fischer,Rainer Haas,Hong Tang,Barry J Marshall,Mohammed Benghezal
Protein glycosylation has been considered as a fundamental phenomenon shared by all domains of life. In Helicobacter pylori, glycosylation of flagellins A and B with pseudaminic acid have been rigorously confirmed and shown to be essential for flagella assembly and bacterial colonization. In addition to flagellins, several other proteins including RecA, AlpA/B, and BabA/B in H. pylori have also been
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Epidemiology of bacterial biofilms on polyps and normal tissues in a screening colonoscopy cohort. Gut Microbes (IF 12.2) Pub Date : 2025-01-18 Julia L Drewes,Samara B Rifkin,Madison McMann,Sara Glass,Emma Spence,Caroline R Wensel,Abby L Geis,Courtney Stevens,Joell J Gills,Hao Wang,Linda M Hylind,Gerard Mullin,David Kafonek,David Cromwell,Louis La Luna,Francis M Giardiello,Cynthia L Sears,,
BACKGROUND Invasive bacterial biofilms are implicated in colorectal cancer. However, their prevalence on histologically normal tissues and polyps is not well established, and risk factors of biofilms have not been previously investigated. Here we evaluated potential procedural and demographic risk factors associated with biofilm status using a cross-sectional observational cohort. METHODS Histologically
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From chaos to order: optimizing fecal microbiota transplantation for enhanced immune checkpoint inhibitors efficacy. Gut Microbes (IF 12.2) Pub Date : 2025-01-18 Anqi Lin,Aimin Jiang,Lihaoyun Huang,Yu Li,Chunyanx Zhang,Lingxuan Zhu,Weiming Mou,Zaoqu Liu,Jian Zhang,Quan Cheng,Ting Wei,Peng Luo
The integration of fecal microbiota transplantation (FMT) with immune checkpoint inhibitors (ICIs) presents a promising approach for enhancing cancer treatment efficacy and overcoming therapeutic resistance. This review critically examines the controversial effects of FMT on ICIs outcomes and elucidates the underlying mechanisms. We investigate how FMT modulates gut microbiota composition, microbial
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Digging deeper into necrotizing enterocolitis: bridging clinical, microbial, and molecular perspectives. Gut Microbes (IF 12.2) Pub Date : 2025-01-18 Deshuang Zhang,Dongke Xie,Yi Qu,Dezhi Mu,Shaopu Wang
Necrotizing Enterocolitis (NEC) is a severe, life-threatening inflammatory condition of the gastrointestinal tract, especially affecting preterm infants. This review consolidates evidence from various biomedical disciplines to elucidate the complex pathogenesis of NEC, integrating insights from clinical, microbial, and molecular perspectives. It emphasizes the modulation of NEC-associated inflammatory
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Limosilactobacillus reuteri - a probiotic gut commensal with contextual impact on immunity. Gut Microbes (IF 12.2) Pub Date : 2025-01-17 Amanda H Lee,Daphne Michelle Rodriguez Jimenez,Marlies Meisel
The gut microbiome plays a key role in human health, influencing various biological processes and disease outcomes. The historical roots of probiotics are traced back to Nobel Laureate Élie Metchnikoff, who linked the longevity of Bulgarian villagers to their consumption of sour milk fermented by Lactobacilli. His pioneering work led to the global recognition of probiotics as beneficial supplements
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Inactivation of glutathione S-transferase alpha 4 blocks Enterococcus faecalis-induced bystander effect by promoting macrophage ferroptosis. Gut Microbes (IF 12.2) Pub Date : 2025-01-16 Yuanyuan Ju,Chunhua Ma,Lin Huang,Yumei Tao,Tianqi Li,Haibo Li,Mark M Huycke,Yonghong Yang,Xingmin Wang
Enterococcus faecalis-infected macrophages produce 4-hydroxynonenal (4-HNE) that mediates microbiota-induced bystander effect (MIBE) leading to colorectal cancer (CRC). Glutathione S-transferase alpha 4 (Gsta4), a specific detoxifying enzyme for 4-HNE, is overexpressed in human CRC and E. faecalis-induced murine CRC. However, the roles of Gsta4 in E. faecalis-induced colitis and CRC remain unclear
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Synergistic and off-target effects of bacteriocins in a simplified human intestinal microbiome: implications for Clostridioides difficile infection control. Gut Microbes (IF 12.2) Pub Date : 2025-01-16 Natalia S Ríos Colombo,R Paul Ross,Colin Hill
Clostridioides difficile is a major cause of nosocomial diarrhea. As current antibiotic treatment failures and recurrence of infections are highly frequent, alternative strategies are needed for the treatment of this disease. This study explores the use of bacteriocins, specifically lacticin 3147 and pediocin PA-1, which have reported inhibitory activity against C. difficile. We engineered Lactococcus
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Lactiplantibacillus plantarum (CECT7484 and CECT7485) and Pedioccoccus acidilactici (CECT7483) enhance actin cytoskeleton and CYP1A1 expression restoring epithelial permeability alterations induced by irritable bowel syndrome mediators. Gut Microbes (IF 12.2) Pub Date : 2025-01-16 Maria Raffaella Barbaro,Francesca Bianco,Cesare Cremon,Giovanni Marasco,Francesca Bonomini,Marta Palombo,Cecilia Delprete,Marta Perez,Jordi Espadaler-Mazo,Vincenzo Stanghellini,Simone Guglielmetti,Giovanni Barbara
Irritable bowel syndrome (IBS) is a multifactorial condition with heterogeneous pathophysiology, including intestinal permeability alterations. The aim of the present study was to assess the ability of a probiotic blend (PB) consisting of two Lactiplantibacillus plantarum strains (CECT7484 and CECT7485) and one strain of Pediococcus acidilactici (CECT7483) to recover the permeability increase induced
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Single-cell microbiota phenotyping reveals distinct disease and therapy-associated signatures in Crohn's disease. Gut Microbes (IF 12.2) Pub Date : 2025-01-15 Lisa Budzinski,Gi-Ung Kang,René Riedel,Toni Sempert,Leonie Lietz,René Maier,Janine Büttner,Bettina Bochow,Marcell T Tordai,Aayushi Shah,Amro Abbas,Tanisha Momtaz,Jannike L Krause,Robin Kempkens,Katrin Lehman,Gitta A Heinz,Anne E Benken,Stefanie Bartsch,Kathleen Necke,Ute Hoffmann,Mir-Farzin Mashreghi,Robert Biesen,Tilmann Kallinich,Tobias Alexander,Bosse Jessen,Carl Weidinger,Britta Siegmund,Andreas
IgA-coated fractions of the intestinal microbiota of Crohn's disease (CD) patients have been shown to contain taxa that hallmark the compositional dysbiosis in CD microbiomes. However, the correlation between other cellular properties of intestinal bacteria and disease has not been explored further, especially for features that are not directly driven by the host immune-system, e.g. the expression
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Host-microbe multi-omics and succinotype profiling have prognostic value for future relapse in patients with inflammatory bowel disease. Gut Microbes (IF 12.2) Pub Date : 2025-01-15 Jill O'Sullivan,Shriram Patel,Gabriel E Leventhal,Rachel S Fitzgerald,Emilio J Laserna-Mendieta,Chloe E Huseyin,Nina Konstantinidou,Erica Rutherford,Aonghus Lavelle,Karim Dabbagh,Todd Z DeSantis,Fergus Shanahan,Andriy Temko,Shoko Iwai,Marcus J Claesson
Crohn's disease (CD) and ulcerative colitis (UC) are chronic relapsing inflammatory bowel disorders (IBD), the pathogenesis of which is uncertain but includes genetic susceptibility factors, immune-mediated tissue injury and environmental influences, most of which appear to act via the gut microbiome. We hypothesized that host-microbe alterations could be used to prognostically stratify patients experiencing
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Microbial succinate promotes the response to metformin by upregulating secretory immunoglobulin a in intestinal immunity. Gut Microbes (IF 12.2) Pub Date : 2025-01-15 Ying Zhang,Aiting Wang,Wei Zhao,Jia'an Qin,Yu Zhang,Bing Liu,Chengcheng Yao,Jianglan Long,Mingxia Yuan,Dan Yan
Metformin is the first-line pharmacotherapy for type 2 diabetes mellitus; however, many patients respond poorly to this drug in clinical practice. The potential involvement of microbiota-mediated intestinal immunity and related signals in metformin responsiveness has not been previously investigated. In this study, we successfully constructed a humanized mouse model by fecal transplantation of the
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Gut-derived lactic acid enhances tryptophan to 5-hydroxytryptamine in regulation of anxiety via Akkermansia muciniphila. Gut Microbes (IF 12.2) Pub Date : 2025-01-09 Miaomiao Pan,Chenglang Qian,Shaoye Huo,Yuchen Wu,Xinyi Zhao,Yueming Ying,Boyu Wang,Hao Yang,Anaguli Yeerken,Tongyao Wang,Mengwei Fu,Lihong Wang,Yuhuan Wei,Yunhua Zhao,Chunhai Shao,Huijing Wang,Chao Zhao
The gut microbiota plays a pivotal role in anxiety regulation through pathways involving neurotransmitter production, immune signaling, and metabolic interactions. Among these, gut-derived serotonin (5-hydroxytryptamine, 5-HT), synthesized from tryptophan metabolism, has been identified as a key mediator. However, it remains unclear whether specific microbial factors regulate tryptophan metabolism
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The microbiota-derived bile acid taurodeoxycholic acid improves hepatic cholesterol levels in mice with cancer cachexia. Gut Microbes (IF 12.2) Pub Date : 2025-01-08 Morgane M Thibaut,Martin Roumain,Edwige Piron,Justine Gillard,Axelle Loriot,Audrey M Neyrinck,Julie Rodriguez,Isabelle Massart,Jean-Paul Thissen,Joshua R Huot,Fabrizio Pin,Andrea Bonetto,Nathalie M Delzenne,Giulio G Muccioli,Laure B Bindels
Alterations in bile acid profile and pathways contribute to hepatic inflammation in cancer cachexia, a syndrome worsening the prognosis of cancer patients. As the gut microbiota impinges on host metabolism through bile acids, the current study aimed to explore the functional contribution of gut microbial dysbiosis to bile acid dysmetabolism and associated disorders in cancer cachexia. Using three mouse
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Lactobacillus intestinalis facilitates tumor-derived CCL5 to recruit dendritic cell and suppress colorectal tumorigenesis. Gut Microbes (IF 12.2) Pub Date : 2025-01-08 Yong Sun,Qiwen Wang,Yao Jiang,Jiamin He,Dingjiacheng Jia,Man Luo,Wentao Shen,Qingyi Wang,Yadong Qi,Yifeng Lin,Ying Zhang,Lan Wang,Liangjing Wang,Shujie Chen,Lina Fan
Gut microbes play a crucial role in regulating the tumor microenvironment (TME) of colorectal cancer (CRC). Nevertheless, the deep mechanism between the microbiota-TME interaction has not been well explored. In this study, we for the first time discovered that Lactobacillus intestinalis (L. intestinalis) effectively suppressed tumor growth both in the AOM/DSS-induced CRC model and the ApcMin/+ spontaneous
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Nutritional optimization of fecal microbiota transplantation in humans: a scoping review. Gut Microbes (IF 12.2) Pub Date : 2025-01-08 Levi M Teigen,Austin Hoeg,Hijab Zehra,Priyali Shah,Remy Johnson,Kristen Hutchison,Megan Kocher,Annie W Lin,Abigail J Johnson,Byron P Vaughn
Diet constitutes a major source of nutrient flow to the gut microbes. As such, it can be used to help shape the gut microbiome. Fecal microbiota transplantation (FMT) is an increasingly promising therapy in disease states beyond recurrent Clostridioides difficile infection, but diet is largely overlooked for its potential to help optimize this therapy. Therefore, the aim of this scoping review is to
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Mouse strain-specific responses along the gut-brain axis upon fecal microbiota transplantation from children with autism. Gut Microbes (IF 12.2) Pub Date : 2025-01-07 Naika Prince,Lucia N Peralta Marzal,Léa Roussin,Magali Monnoye,Catherine Philippe,Elise Maximin,Sabbir Ahmed,Karoliina Salenius,Jake Lin,Reija Autio,Youri Adolfs,R Jeroen Pasterkamp,Johan Garssen,Laurent Naudon,Sylvie Rabot,Aletta D Kraneveld,Paula Perez-Pardo
Several factors are linked to the pathophysiology of autism spectrum disorders (ASD); however, the molecular mechanisms of the condition remain unknown. As intestinal problems and gut microbiota dysbiosis are associated with ASD development and severity, recent studies have focused on elucidating the microbiota-gut-brain axis' involvement. This study aims to explore mechanisms through which gut microbiota
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Gut dysbiosis-induced vitamin B6 metabolic disorder contributes to chronic stress-related abnormal behaviors in a cortisol-independent manner. Gut Microbes (IF 12.2) Pub Date : 2025-01-07 Wenxiang Qing,Huimin Chen,Xin Ma,Jie Chen,Yuan Le,Hui Chen,Jianhua Tong,Kaiming Duan,Daqing Ma,Wen Ouyang,Jianbin Tong
Chronic stress can result in various conditions, including psychological disorders, neurodegenerative diseases, and accelerated brain aging. Gut dysbiosis potentially contributes to stress-related brain disorders in individuals with chronic stress. However, the causal relationship and key factors between gut dysbiosis and brain disorders in chronic stress remain elusive, particularly under non-sterile
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Gut microbes metabolize strawberry phytochemicals and mediate their beneficial effects on vascular inflammation. Gut Microbes (IF 12.2) Pub Date : 2025-01-06 Chrissa Petersen,Adhini Kuppuswamy Satheesh Babu,Ceres Mattos Della Lucia,Henry A Paz,Lisard Iglesias-Carres,Ying Zhong,Thunder Jalili,J David Symons,Kartik Shankar,Andrew P Neilson,Umesh D Wankhade,Pon Velayutham Anandh Babu
Evidence suggests that a healthy gut microbiome is essential for metabolizing dietary phytochemicals. However, the microbiome's role in metabolite production and the influence of gut dysbiosis on this process remain unclear. Further, studies on the relationship among gut microbes, metabolites, and biological activities of phytochemicals are limited. We addressed this knowledge gap using strawberry
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Hypoglycemic effect of C. butyricum-pMTL007-GLP-1 engineered probiotics on type 2 diabetes mellitus. Gut Microbes (IF 12.2) Pub Date : 2025-01-02 Dexi Zhou,Shengjie Li,Gang Hu,Yufan Wang,Zhanghua Qi,Xuan Xu,Jing Wei,Qiong Liu,Tingtao Chen
Diabetes mellitus (DM) is a complex metabolic disease characterized by hyperglycemia. Recently, the incidence of diabetes has increased exponentially, and it is estimated to become the seventh leading cause of global mortality by 2030. Glucagon-like peptide-1 (GLP-1), a hormone derived from the intestine, has been demonstrated to exert remarkable hypoglycemic effects. However, its limitation lies in
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Breastfeeding and infant gut microbiota: influence of bioactive components. Gut Microbes (IF 12.2) Pub Date : 2024-12-27 Dongqing Xu,Fengyi Wan
Establishment of the gut microbiota during infancy is critical for host health with long-lasting implications. In this orchestrated process, microbial assembly is influenced by an increasing number of genetic and environmental factors, among which breastfeeding is considered as one of the most significant drivers for infant gut microbiota development. As the optimal diet for the infants, maternal milk
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Gut microbiota-derived 4-hydroxyphenylacetic acid from resveratrol supplementation prevents obesity through SIRT1 signaling activation. Gut Microbes (IF 12.2) Pub Date : 2024-12-26 Pan Wang,Ruiqi Wang,Wenting Zhao,Yuanyuan Zhao,Dan Wang,Shuang Zhao,Zhiwen Ge,Yue Ma,Xiaoyan Zhao
Resveratrol (RSV), a natural polyphenol, has been suggested to influence glucose and lipid metabolism. However, the underlying molecular mechanism of its action remains largely unknown due to its multiple biological targets and low bioavailability. In this study, we demonstrate that RSV supplementation ameliorates high-fat-diet (HFD)-induced gut microbiota dysbiosis, enhancing the abundance of anti-obesity
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Gut microbes with the gbu genes determine TMAO production from L-carnitine intake and serve as a biomarker for precision nutrition. Gut Microbes (IF 12.2) Pub Date : 2024-12-26 Wei-Kai Wu,Yi-Ling Lo,Jian-Ying Chiu,Chia-Lang Hsu,I-Hsuan Lo,Suraphan Panyod,Yu-Chieh Liao,Tina H T Chiu,Yu-Tang Yang,Han-Chun Kuo,Hsin-Bai Zou,Yi-Hsun Chen,Hsiao-Li Chuang,Jeffrey J Y Yen,Jin-Town Wang,Han-Mo Chiu,Cheng-Chih Hsu,Ching-Hua Kuo,Lee-Yan Sheen,Hsien-Li Kao,Ming-Shiang Wu
Gut microbial metabolism of L-carnitine, which leads to the production of detrimental trimethylamine N-oxide (TMAO), offers a plausible link between red meat consumption and cardiovascular risks. Several microbial genes, including cntA/B, the cai operon, and the recently identified gbu gene cluster, have been implicated in the conversion of dietary L-carnitine into TMA(O). However, the key microbial
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Desulfovibrio vulgaris flagellin exacerbates colorectal cancer through activating LRRC19/TRAF6/TAK1 pathway. Gut Microbes (IF 12.2) Pub Date : 2024-12-24 Yue Dong,Fanyi Meng,Jingyi Wang,Jingge Wei,Kexin Zhang,Siqi Qin,Mengfan Li,Fucheng Wang,Bangmao Wang,Tianyu Liu,Weilong Zhong,Hailong Cao
The initiation and progression of colorectal cancer (CRC) are intimately associated with genetic, environmental and biological factors. Desulfovibrio vulgaris (DSV), a sulfate-reducing bacterium, has been found excessive growth in CRC patients, suggesting a potential role in carcinogenesis. However, the precise mechanisms underlying this association remain incompletely understood. We have found Desulfovibrio
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Identifying the location-dependent adipose tissue bacterial DNA signatures in obese patients that predict body weight loss. Gut Microbes (IF 12.2) Pub Date : 2024-12-23 Matthieu Minty,Alberic Germain,Jiuwen Sun,Gracia Kaglan,Florence Servant,Benjamin Lelouvier,Emiri Misselis,Radu Mircea Neagoe,Menghini Rossella,Marina Cardellini,Rémy Burcelin,Massimo Federici,José Manuel Fernandez-Real,Vincent Blasco-Baque
Recent sets of evidence have described profiles of 16S rDNA sequences in host tissues, notably in fat pads that are significantly overrepresented and can serve as signatures of metabolic disease. However, these recent and original observations need to be further detailed and functionally defined. Here, using state-of-the-art targeted DNA sequencing and discriminant predictive approaches, we describe
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Development of a Caco-2-based intestinal mucosal model to study intestinal barrier properties and bacteria-mucus interactions. Gut Microbes (IF 12.2) Pub Date : 2024-12-23 Evelien Floor,Jinyi Su,Maitrayee Chatterjee,Elise S Kuipers,Noortje IJssennagger,Faranak Heidari,Laura Giordano,Richard W Wubbolts,Silvia M Mihăilă,Daphne A C Stapels,Yvonne Vercoulen,Karin Strijbis
The intestinal mucosal barrier is a dynamic system that allows nutrient uptake, stimulates healthy microbe-host interactions, and prevents invasion by pathogens. The mucosa consists of epithelial cells connected by cellular junctions that regulate the passage of nutrients covered by a mucus layer that plays an important role in host-microbiome interactions. Mimicking the intestinal mucosa for in vitro
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A modelling framework to characterize the impact of antibiotics on the gut microbiota diversity. Gut Microbes (IF 12.2) Pub Date : 2024-12-22 Carlos Olivares,Etienne Ruppé,Stéphanie Ferreira,Tanguy Corbel,Antoine Andremont,Jean de Gunzburg,Jeremie Guedj,Charles Burdet
Metagenomic sequencing deepened our knowledge about the role of the intestinal microbiota in human health, and several studies with various methodologies explored its dynamics during antibiotic treatments. We compared the impact of four widely used antibiotics on the gut bacterial diversity. We used plasma and fecal samples collected during and after treatment from healthy volunteers assigned to a
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Maternal probiotic exposure enhances CD8 T cell protective neonatal immunity and modulates offspring metabolome to control influenza virus infection. Gut Microbes (IF 12.2) Pub Date : 2024-12-22 Clara Valentin,Patricia Brito Rodrigues,Marko Verce,Sandrine Delbauve,Léa La Palombara,Florine Demaret,Justine Allard,Isabelle Salmon,Patrice D Cani,Arnaud Köhler,Amandine Everard,Véronique Flamand
Maternal gut microbiota composition contributes to the status of the neonatal immune system and could influence the early life higher susceptibility to viral respiratory infections. Using a novel protocol of murine maternal probiotic supplementation, we report that perinatal exposure to Lacticaseibacillus rhamnosus (L.rh) or Bifidobacterium animalis subsp. lactis (B.lac) increases the influenza A/PR8
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Fusobacterium nucleatum-driven CX3CR1+ PD-L1+ phagocytes route to tumor tissues and reshape tumor microenvironment. Gut Microbes (IF 12.2) Pub Date : 2024-12-22 Fangfang Chen,Songhe Guo,Yiqiu Li,Yongfan Lu,Le Liu,Shengxin Chen,Jun An,Ge Zhang
The intracellular bacterium Fusobacterium nucleatum (Fn) mediates tumorigenesis and progression in colorectal cancer (CRC). However, the origin of intratumoral Fn and the role of Fn-infected immunocytes in the tumor microenvironment remain unclear. Here, we observed that Fn-infected neutrophils/macrophages (PMNs/MΦs), especially PMNs, accumulate in tumor tissues and fecal Fn abundance correlates positively