With the rise of fluorescence-guided surgery, it has become evident that different types of fluorescence signals can provide value in the surgical setting. Hereby a different range of targets have been pursued in a great variety of surgical indications. One of the future challenges lies in combining complementary fluorescent readouts during one and the same surgical procedure, so-called multi-wavelength fluorescence guidance. In this review we summarize the current clinical state-of-the-art in multi-wavelength fluorescence guidance, basic technical concepts, possible future extensions of existing clinical indications and impact that the technology can bring to clinical care.

随着荧光引导手术的兴起，很明显不同类型的荧光信号可以在手术环境中提供价值。因此，在各种各样的手术适应症中追求了不同范围的目标。未来的挑战之一在于在同一个手术过程中结合互补荧光读数，即所谓的多波长荧光引导。在这篇综述中，我们总结了多波长荧光引导的当前临床最新技术、基本技术概念、现有临床适应症的未来可能扩展以及该技术对临床护理的影响。