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Magnetofluorescent Nanoprobe for Multimodal and Multicolor Bioimaging
Molecular Imaging ( IF 2.8 ) Pub Date : 2020-10-28 , DOI: 10.1177/1536012120969477
Aditya Yadav 1 , Chethana Rao 1 , Navneet Chandra Verma 1 , Pushpendra Mani Mishra 1, 2 , Chayan Kanti Nandi 1, 2, 3
Affiliation  

Although, superparamagnetic iron oxide nanoparticles (SPIONs) have extensively been used as a contrasting agent for magnetic resonance imaging (MRI), the lack of intrinsic fluorescence restricted their application as a multimodal probe, especially in combination with light microscopy. In Addition, the bigger size of the particle renders them incompetent for bioimaging of small organelles. Herein, we report, not only the synthesis of ultrasmall carbon containing magneto-fluorescent SPIONs with size ∼5 nm, but also demonstrate its capability as a multicolor imaging probe. Using MCF-7 and HeLa cell lines, we show that the SPIONs can provide high contrast mulicolor images of the cytoplasm from blue to red region. Further, single particle level photon count data revealed that the SPIONs could efficaciously be utilized in localization based super resolution microscopy in future.



中文翻译:

用于多模式和多色生物成像的磁荧光纳米探针

尽管超顺磁性氧化铁纳米颗粒 (SPIONs) 已广泛用作磁共振成像 (MRI) 的造影剂,但缺乏固有荧光限制了它们作为多模式探针的应用,特别是与光学显微镜结合使用。此外,较大尺寸的颗粒使它们无法对小细胞器进行生物成像。在此,我们报告了不仅合成了尺寸约为 5 nm 的超小含碳磁荧光 SPION,而且还展示了其作为多色成像探针的能力。使用 MCF-7 和 HeLa 细胞系,我们表明 SPION 可以提供从蓝色到红色区域的细胞质的高对比度多色图像。进一步,

更新日期:2020-10-29
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