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CCL5 is essential for axonogenesis and neuronal restoration after brain injury J. Biomed. Sci. (IF 9.0) Pub Date : 2024-09-17 Man-Hau Ho, Yih-Jeng Tsai, Chia-Yen Chen, Anastasia Yang, Thierry Burnouf, Yun Wang, Yung-Hsiao Chiang, Barry J. Hoffer, Szu-Yi Chou
Traumatic brain injury (TBI) causes axon tearing and synapse degradation, resulting in multiple neurological dysfunctions and exacerbation of early neurodegeneration; the repair of axonal and synaptic structures is critical for restoring neuronal function. C-C Motif Chemokine Ligand 5 (CCL5) shows many neuroprotective activities. A close-head weight-drop system was used to induce mild brain trauma
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Decreased plasma gelsolin fosters a fibrotic tumor microenvironment and promotes chemoradiotherapy resistance in esophageal squamous cell carcinoma J. Biomed. Sci. (IF 9.0) Pub Date : 2024-09-11 Chih-Hsiung Hsieh, Pei-Shiuan Ho, Wen-Lun Wang, Fu-Hsuan Shih, Chen-Tai Hong, Pei-Wen Wang, Dar-Bin Shieh, Wei-Lun Chang, Yi-Ching Wang
Stromal fibrosis is highly associated with therapeutic resistance and poor survival in esophageal squamous cell carcinoma (ESCC) patients. Low expression of plasma gelsolin (pGSN), a serum abundant protein, has been found to correlate with inflammation and fibrosis. Here, we evaluated pGSN expression in patients with different stages of cancer and therapeutic responses, and delineated the molecular
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Current landscape of mRNA technologies and delivery systems for new modality therapeutics J. Biomed. Sci. (IF 9.0) Pub Date : 2024-09-10 Ruei-Min Lu, Hsiang-En Hsu, Ser John Lynon P. Perez, Monika Kumari, Guan-Hong Chen, Ming-Hsiang Hong, Yin-Shiou Lin, Ching-Hang Liu, Shih-Han Ko, Christian Angelo P. Concio, Yi-Jen Su, Yi-Han Chang, Wen-Shan Li, Han-Chung Wu
Realizing the immense clinical potential of mRNA-based drugs will require continued development of methods to safely deliver the bioactive agents with high efficiency and without triggering side effects. In this regard, lipid nanoparticles have been successfully utilized to improve mRNA delivery and protect the cargo from extracellular degradation. Encapsulation in lipid nanoparticles was an essential
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Tudor-SN exacerbates pathological vascular remodeling by promoting the polyubiquitination of PTEN via NEDD4-1 J. Biomed. Sci. (IF 9.0) Pub Date : 2024-09-05 Yichen Wu, Zilong Chen, Zhe Zheng, Xiaoguang Li, Jiangcheng Shu, Ruiqi Mao, Jie An, Siyuan Fan, Ruijie Luo, Yi Guo, Wenjing Xu, Minglu Liang, Kai Huang, Cheng Wang
Dysregulation of vascular homeostasis can induce cardiovascular diseases and increase global mortality rates. Although lineage tracing studies have confirmed the pivotal role of modulated vascular smooth muscle cells (VSMCs) in the progression of pathological vascular remodeling, the underlying mechanisms are still unclear. The expression of Tudor-SN was determined in VSMCs of artery stenosis, PDGF-BB-treated
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Neuroprotective effects of intranasal extracellular vesicles from human platelet concentrates supernatants in traumatic brain injury and Parkinson’s disease models J. Biomed. Sci. (IF 9.0) Pub Date : 2024-09-05 Liling Delila, Ouada Nebie, Nhi Thao Ngoc Le, Kelly Timmerman, Deng-Yao Lee, Yu-Wen Wu, Ming-Li Chou, Luc Buée, Szu-Yi Chou, David Blum, David Devos, Thierry Burnouf
The burgeoning field of regenerative medicine has significantly advanced with recent findings on biotherapies using human platelet lysates (HPLs), derived from clinical-grade platelet concentrates (PCs), for treating brain disorders. These developments have opened new translational research avenues to explore the neuroprotective effects of platelet-extracellular vesicles (PEVs). Their potential in
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Dengue NS1 interaction with lipids alters its pathogenic effects on monocyte derived macrophages J. Biomed. Sci. (IF 9.0) Pub Date : 2024-09-04 Shashika Dayarathna, Bhagya Senadheera, Chandima Jeewandara, Madushika Dissanayake, Farha Bary, Graham S. Ogg, Gathsaurie Neelika Malavige
While dengue NS1 antigen has been shown to be associated with disease pathogenesis in some studies, it has not been linked in other studies, with the reasons remaining unclear. NS1 antigen levels in acute dengue are often associated with increased disease severity, but there has been a wide variation in results based on past dengue infection and infecting dengue virus (DENV) serotype. As NS1 engages
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Extracellular vesicle therapy in neurological disorders J. Biomed. Sci. (IF 9.0) Pub Date : 2024-08-25 Napasiri Putthanbut, Jea Young Lee, Cesario V. Borlongan
Extracellular vesicles (EVs) are vital for cell-to-cell communication, transferring proteins, lipids, and nucleic acids in various physiological and pathological processes. They play crucial roles in immune modulation and tissue regeneration but are also involved in pathogenic conditions like inflammation and degenerative disorders. EVs have heterogeneous populations and cargo, with numerous subpopulations
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Machine learning enabled classification of lung cancer cell lines co-cultured with fibroblasts with lightweight convolutional neural network for initial diagnosis J. Biomed. Sci. (IF 9.0) Pub Date : 2024-08-23 Adam Germain, Alex Sabol, Anjani Chavali, Giles Fitzwilliams, Alexa Cooper, Sandra Khuon, Bailey Green, Calvin Kong, John Minna, Young-Tae Kim
Identification of lung cancer subtypes is critical for successful treatment in patients, especially those in advanced stages. Many advanced and personal treatments require knowledge of specific mutations, as well as up- and down-regulations of genes, for effective targeting of the cancer cells. While many studies focus on individual cell structures and delve deeper into gene sequencing, the present
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Beyond glycan barriers: non-cognate ligands and protein mimicry approaches to elicit broadly neutralizing antibodies for HIV-1 J. Biomed. Sci. (IF 9.0) Pub Date : 2024-08-21 Stephen Ian Walimbwa, Petr Maly, Leona Raskova Kafkova, Milan Raska
Human immunodeficiency virus type 1 (HIV-1) vaccine immunogens capable of inducing broadly neutralizing antibodies (bNAbs) remain obscure. HIV-1 evades immune responses through enormous diversity and hides its conserved vulnerable epitopes on the envelope glycoprotein (Env) by displaying an extensive immunodominant glycan shield. In elite HIV-1 viremic controllers, glycan-dependent bNAbs targeting
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Plexin C1 influences immune response to intracellular LPS and survival in murine sepsis J. Biomed. Sci. (IF 9.0) Pub Date : 2024-08-21 Alice Bernard, Claudia Eggstein, Linyan Tang, Marius Keller, Andreas Körner, Valbona Mirakaj, Peter Rosenberger
Intracellular sensing of lipopolysaccharide (LPS) is essential for the immune response against gram-negative bacteria and results in activation of caspase-11 and pyroptotic cell death with fatal consequences in sepsis. We found the neuronal guidance receptor plexin C1 (PLXNC1) influences the intracellular response to LPS. We employed a murine model of sepsis via cecal ligation and binding (CLP), using
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EpCAM-targeted betulinic acid analogue nanotherapy improves therapeutic efficacy and induces anti-tumorigenic immune response in colorectal cancer tumor microenvironment J. Biomed. Sci. (IF 9.0) Pub Date : 2024-08-20 Debasmita Dutta, Ashique Al Hoque, Brahamacharry Paul, Jun Hyoung Park, Chinmay Chowdhury, Mohiuddin Quadir, Soumyabrata Banerjee, Arghadip Choudhury, Soumik Laha, Nayim Sepay, Priyanka Boro, Benny Abraham Kaipparettu, Biswajit Mukherjee
Betulinic acid (BA) has been well investigated for its antiproliferative and mitochondrial pathway-mediated apoptosis-inducing effects on various cancers. However, its poor solubility and off-target activity have limited its utility in clinical trials. Additionally, the immune modulatory role of betulinic acid analogue in the tumor microenvironment (TME) is largely unknown. Here, we designed a potential
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Low-level HIV-1 viremia affects T-cell activation and senescence in long-term treated adults in the INSTI era J. Biomed. Sci. (IF 9.0) Pub Date : 2024-08-19 Violeta Lara-Aguilar, Manuel Llamas-Adán, Óscar Brochado-Kith, Celia Crespo-Bermejo, Sergio Grande-García, Sonia Arca-Lafuente, Ignacio de los Santos, Carmen Prado, Mario Alía, Coral Sainz-Pinós, Amanda Fernández-Rodríguez, Luz Martín-Carbonero, Ricardo Madrid, Verónica Briz
Around 10% of people with HIV (PWH) exhibit a low-level viremia (LLV) under antiretroviral therapy (ART). However, its origin and clinical significance are largely unknown, particularly at viremias between 50 and 200 copies/mL and under modern ART based on integrase strand transfer inhibitors (INSTIs). Our aim was to characterize their poor immune response against HIV in comparison to individuals with
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Gene therapy for ultrarare diseases: a geneticist’s perspective J. Biomed. Sci. (IF 9.0) Pub Date : 2024-08-13 Wuh-Liang Hwu
Gene therapy has made considerable strides in recent years. More than 4000 protein-coding genes have been implicated in more than 6000 genetic diseases; next-generation sequencing has dramatically revolutionized the diagnosis of genetic diseases. Most genetic diseases are considered very rare or ultrarare, defined here as having fewer than 1:100,000 cases, but only one of the 12 approved gene therapies
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Targeted nanotherapeutics for the treatment of Helicobacter pylori infection J. Biomed. Sci. (IF 9.0) Pub Date : 2024-08-11 Rute Chitas, Diana R. Fonseca, Paula Parreira, M. Cristina L. Martins
Helicobacter pylori infection is involved in gastric diseases such as peptic ulcer and adenocarcinoma. Approved antibiotherapies still fail in 10 to 40% of the infected patients and, in this scenario, targeted nanotherapeutics emerged as powerful allies for H. pylori eradication. Nano/microparticles conjugated with H. pylori binding molecules were developed to eliminate H. pylori by either (i) blocking
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Variants of human DECTIN-1 rs16910526 are linked to differential reactive oxygen species production and susceptibility to tuberculosis J. Biomed. Sci. (IF 9.0) Pub Date : 2024-08-05 Mónica Cufré, Mercedes Pastorini, Ignacio Martín, Rodrigo Failde, Domingo Palmero, Mercedes Alemán
Dectin-1 is a transmembrane receptor that plays a pivotal role in recognising fungi and Mycobacterium tuberculosis (Mtb). A specific variant, DECTIN-1 rs16910526, results in a truncated receptor that disrupts membrane expression and ligand binding and is clinically associated with recurrent cutaneous mycoses. Previous research has clarified the role of Dectin-1 in boosting immune defenses against mycobacteria
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Retraction Note: Inhibitors of apoptosis proteins in experimental benign prostatic hyperplasia: effects of Serenoa repens, selenium and lycopene J. Biomed. Sci. (IF 9.0) Pub Date : 2024-08-02 Letteria Minutoli, Domenica Altavilla, Herbert Marini, Mariagrazia Rinaldi, Natasha Irrera, Gabriele Pizzino, Alessandra Bitto, Salvatore Arena, Sebastiano Cimino, Francesco Squadrito, Giorgio Ivan Russo, Giuseppe Morgia
Retraction Note: Journal of Biomedical Science (2014) 21:19 http://www.jbiomedsci.com/content/21/1/19 The Editor-in-Chief retracted this article because of concerns regarding a number of figures presented in this work. These concerns call into question the article's overall scientific soundness. An investigation conducted after its publication discovered similarities in the six gels presented in Figure 1
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Development of novel antimicrobials with engineered endolysin LysECD7-SMAP to combat Gram-negative bacterial infections J. Biomed. Sci. (IF 9.0) Pub Date : 2024-07-24 Daria V. Vasina, Nataliia P. Antonova, Vladimir A. Gushchin, Andrey V. Aleshkin, Mikhail V. Fursov, Anastasiia D. Fursova, Petya G. Gancheva, Igor V. Grigoriev, Pavel Grinkevich, Alexey V. Kondratev, Alexey V. Kostarnoy, Anastasiya M. Lendel, Valentine V. Makarov, Maria A. Nikiforova, Andrei A. Pochtovyi, Tatiana Prudnikova, Timofey A. Remizov, Natalia V. Shevlyagina, Andrei E. Siniavin, Nina S. Smirnova
Among the non-traditional antibacterial agents in development, only a few targets critical Gram-negative bacteria such as carbapenem-resistant Pseudomonas aeruginosa, Acinetobacter baumannii or cephalosporin-resistant Enterobacteriaceae. Endolysins and their genetically modified versions meet the World Health Organization criteria for innovation, have a novel mode of antibacterial action, no known
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BUB1B monoallelic germline variants contribute to prostate cancer predisposition by triggering chromosomal instability J. Biomed. Sci. (IF 9.0) Pub Date : 2024-07-16 Maria P. Silva, Luísa T. Ferreira, Natércia F. Brás, Lurdes Torres, Andreia Brandão, Manuela Pinheiro, Marta Cardoso, Adriana Resende, Joana Vieira, Carlos Palmeira, Gabriela Martins, Miguel Silva, Carla Pinto, Ana Peixoto, João Silva, Rui Henrique, Sofia Maia, Helder Maiato, Manuel R. Teixeira, Paula Paulo
Prostate cancer (PrCa) is the most frequently diagnosed cancer in men. Variants in known moderate- to high-penetrance genes explain less than 5% of the cases arising at early-onset (< 56 years) and/or with familial aggregation of the disease. Considering that BubR1 is an essential component of the mitotic spindle assembly checkpoint, we hypothesized that monoallelic BUB1B variants could be sufficient
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Enteroviruses: epidemic potential, challenges and opportunities with vaccines J. Biomed. Sci. (IF 9.0) Pub Date : 2024-07-15 Minne Jartti, Malin Flodström-Tullberg, Minna M. Hankaniemi
Enteroviruses (EVs) are the most prevalent viruses in humans. EVs can cause a range of acute symptoms, from mild common colds to severe systemic infections such as meningitis, myocarditis, and flaccid paralysis. They can also lead to chronic diseases such as cardiomyopathy. Although more than 280 human EV serotypes exist, only four serotypes have licenced vaccines. No antiviral drugs are available
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Intracellular domain of epithelial cell adhesion molecule induces Wnt receptor transcription to promote colorectal cancer progression J. Biomed. Sci. (IF 9.0) Pub Date : 2024-07-15 Sushree Shankar Panda, Chi-Chiu Lee, Khamushavalli Geevimaan, Kai-Chi Chen, Shung-Haur Yang, Chia-Ning Shen, Wei-Chun HuangFu, Han-Chung Wu
Epithelial cell adhesion molecule (EpCAM) has been widely studied as a tumor antigen due to its expression in varieties of solid tumors. Moreover, the glycoprotein contributes to critical cancer-associated cellular functionalities via its extracellular (EpEX) and intracellular (EpICD) domains. In colorectal cancer (CRC), EpCAM has been implicated in the Wnt signaling pathway, as EpICD and β-Catenin
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Unraveling the differential mechanisms of revascularization promoted by MSCs & ECFCs from adipose tissue or umbilical cord in a murine model of critical limb-threatening ischemia J. Biomed. Sci. (IF 9.0) Pub Date : 2024-07-15 Marta Rojas-Torres, Lucía Beltrán-Camacho, Ana Martínez-Val, Ismael Sánchez-Gomar, Sara Eslava-Alcón, Antonio Rosal-Vela, Margarita Jiménez-Palomares, Esther Doiz-Artázcoz, Mario Martínez-Torija, Rafael Moreno-Luna, Jesper V. Olsen, Ma. Carmen Duran-Ruiz
Critical limb-threatening ischemia (CLTI) constitutes the most severe manifestation of peripheral artery disease, usually induced by atherosclerosis. CLTI patients suffer from high risk of amputation of the lower extremities and elevated mortality rates, while they have low options for surgical revascularization due to associated comorbidities. Alternatively, cell-based therapeutic strategies represent
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A glimpse into viral warfare: decoding the intriguing role of highly pathogenic coronavirus proteins in apoptosis regulation J. Biomed. Sci. (IF 9.0) Pub Date : 2024-07-13 Leyi Cheng, Yajuan Rui, Yanpu Wang, Shiqi Chen, Jiaming Su, Xiao-Fang Yu
Coronaviruses employ various strategies for survival, among which the activation of endogenous or exogenous apoptosis stands out, with viral proteins playing a pivotal role. Notably, highly pathogenic coronaviruses such as SARS-CoV-2, SARS-CoV, and MERS-CoV exhibit a greater array of non-structural proteins compared to low-pathogenic strains, facilitating their ability to induce apoptosis via multiple
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CPEB2-activated axonal translation of VGLUT2 mRNA promotes glutamatergic transmission and presynaptic plasticity J. Biomed. Sci. (IF 9.0) Pub Date : 2024-07-11 Wen-Hsin Lu, Tzu-Tung Chang, Yao-Ming Chang, Yi-Hsiang Liu, Chia-Hsuan Lin, Ching-Shu Suen, Ming-Jing Hwang, Yi-Shuian Huang
Local translation at synapses is important for rapidly remodeling the synaptic proteome to sustain long-term plasticity and memory. While the regulatory mechanisms underlying memory-associated local translation have been widely elucidated in the postsynaptic/dendritic region, there is no direct evidence for which RNA-binding protein (RBP) in axons controls target-specific mRNA translation to promote
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Dual inhibition of SUMOylation and MEK conquers MYC-expressing KRAS-mutant cancers by accumulating DNA damage J. Biomed. Sci. (IF 9.0) Pub Date : 2024-07-11 Hiroshi Kotani, Hiroko Oshima, Justin C. Boucher, Tomoyoshi Yamano, Hiroyuki Sakaguchi, Shigeki Sato, Koji Fukuda, Akihiro Nishiyama, Kaname Yamashita, Koushiro Ohtsubo, Shinji Takeuchi, Takumi Nishiuchi, Masanobu Oshima, Marco L. Davila, Seiji Yano
KRAS mutations frequently occur in cancers, particularly pancreatic ductal adenocarcinoma, colorectal cancer, and non-small cell lung cancer. Although KRASG12C inhibitors have recently been approved, effective precision therapies have not yet been established for all KRAS-mutant cancers. Many treatments for KRAS-mutant cancers, including epigenome-targeted drugs, are currently under investigation.
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Exosomes: a review of biologic function, diagnostic and targeted therapy applications, and clinical trials J. Biomed. Sci. (IF 9.0) Pub Date : 2024-07-11 Yi-Fan Chen, Frank Luh, Yuan-Soon Ho, Yun Yen
Exosomes are extracellular vesicles generated by all cells and they carry nucleic acids, proteins, lipids, and metabolites. They mediate the exchange of substances between cells,thereby affecting biological properties and activities of recipient cells. In this review, we briefly discuss the composition of exocomes and exosome isolation. We also review the clinical applications of exosomes in cancer
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Cholestasis-induced phenotypic transformation of neutrophils contributes to immune escape of colorectal cancer liver metastasis J. Biomed. Sci. (IF 9.0) Pub Date : 2024-06-29 Li Sun, Nanyan Yang, Zhihong Liu, Xiandong Ye, Mengting Cheng, Lingjun Deng, Junhao Zhang, Jingjing Wu, Min Shi, Wangjun Liao
Cholestasis is a common yet severe complication that occurs during the advancement of liver metastasis. However, how cholestasis impacts the development, treatment, and tumor microenvironment (TME) of liver metastasis remains to be elucidated. Extrahepatic and intrahepatic cholestatic mouse models with liver metastasis were established to detect the differential expression levels of genes, infiltration
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Enterovirus-A71 exploits RAB11 to recruit chaperones for virus morphogenesis J. Biomed. Sci. (IF 9.0) Pub Date : 2024-06-28 Qing Yong Ng, Vikneswari Mahendran, Ze Qin Lim, Jasmine Hwee Yee Tan, Joel Jie Feng Wong, Justin Jang Hann Chu, Vincent T. K. Chow, Newman Siu Kwan Sze, Sylvie Alonso
Enterovirus 71 (EV-A71) causes Hand, Foot and Mouth Disease (HFMD) in children and has been associated with neurological complications. The molecular mechanisms involved in EV-A71 pathogenesis have remained elusive. A siRNA screen in EV-A71 infected-motor neurons was performed targeting 112 genes involved in intracellular membrane trafficking, followed by validation of the top four hits using deconvoluted
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The double whammy of ER-retention and dominant-negative effects in numerous autosomal dominant diseases: significance in disease mechanisms and therapy J. Biomed. Sci. (IF 9.0) Pub Date : 2024-06-27 Nesrin Gariballa, Feda Mohamed, Sally Badawi, Bassam R. Ali
The endoplasmic reticulum (ER) employs stringent quality control mechanisms to ensure the integrity of protein folding, allowing only properly folded, processed and assembled proteins to exit the ER and reach their functional destinations. Mutant proteins unable to attain their correct tertiary conformation or form complexes with their partners are retained in the ER and subsequently degraded through
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Beyond traditional translation: ncRNA derived peptides as modulators of tumor behaviors J. Biomed. Sci. (IF 9.0) Pub Date : 2024-06-14 Kang Wen, Xin Chen, Jingyao Gu, Zhenyao Chen, Zhaoxia Wang
Within the intricate tapestry of molecular research, noncoding RNAs (ncRNAs) were historically overshadowed by a pervasive presumption of their inability to encode proteins or peptides. However, groundbreaking revelations have challenged this notion, unveiling select ncRNAs that surprisingly encode peptides specifically those nearing a succinct 100 amino acids. At the forefront of this epiphany stand
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Adipocyte pyroptosis occurs in omental tumor microenvironment and is associated with chemoresistance of ovarian cancer J. Biomed. Sci. (IF 9.0) Pub Date : 2024-06-11 Chang-Ni Lin, Yu-Ling Liang, Hsing-Fen Tsai, Pei-Ying Wu, Lan-Yin Huang, Yu-Han Lin, Chieh-Yi Kang, Chao-Ling Yao, Meng-Ru Shen, Keng-Fu Hsu
Ovarian carcinoma (OC) is a fatal malignancy, with most patients experiencing recurrence and resistance to chemotherapy. In contrast to hematogenous metastasizing tumors, ovarian cancer cells disseminate within the peritoneal cavity, especially the omentum. Previously, we reported omental crown-like structure (CLS) number is associated with poor prognosis of advanced-stage OC. CLS that have pathologic
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Correction: Excess glucose alone depress young mesenchymal stromal/stem cell osteogenesis and mitochondria activity within hours/days via NAD+/ SIRT1 axis J. Biomed. Sci. (IF 9.0) Pub Date : 2024-06-07 B. Linju Yen, Li‑Tzu Wang, Hsiu‑Huang Wang, Chin‑Pao Hung, Pei‑Ju Hsu, Chia‑Chi Chang, Chien‑Yu Liao, Huey‑Kang Sytwu, Men‑Luh Yen
Correction: J Biomed Sci 31, 49 (2024) https://doi.org/10.1186/s12929-024-01039-0 After publication of the article [1], it was brought to our attention that: Figures 2 and Fig. 3 in the original paper still have revision red lettering on some legends. The all-black lettering figures are shown below: Figure 2. Figure 3. In the Funding section, we neglected to update the year-based numbering of two grants
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The glycosylation deficiency of flavivirus NS1 attenuates virus replication through interfering with the formation of viral replication compartments J. Biomed. Sci. (IF 9.0) Pub Date : 2024-06-07 Shuhan Huang, Pan-Deng Shi, Xiao-Xuan Fan, Yang Yang, Cheng-Feng Qin, Hui Zhao, Lei Shi, Yali Ci
Flavivirus is a challenge all over the world. The replication of flavivirus takes place within membranous replication compartments (RCs) derived from endoplasmic reticulum (ER). Flavivirus NS1 proteins have been proven essential for the formation of viral RCs by remodeling the ER. The glycosylation of flavivirus NS1 proteins is important for viral replication, yet the underlying mechanism remains unclear
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Osteosarcoma in a ceRNET perspective J. Biomed. Sci. (IF 9.0) Pub Date : 2024-06-05 Nicola Mosca, Nicola Alessio, Alessandra Di Paola, Maria Maddalena Marrapodi, Umberto Galderisi, Aniello Russo, Francesca Rossi, Nicoletta Potenza
Osteosarcoma (OS) is the most prevalent and fatal type of bone tumor. It is characterized by great heterogeneity of genomic aberrations, mutated genes, and cell types contribution, making therapy and patients management particularly challenging. A unifying picture of molecular mechanisms underlying the disease could help to transform those challenges into opportunities. This review deeply explores
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Immunoglobulin and T cell receptor repertoire changes induced by a prototype vaccine against Chagas disease in naïve rhesus macaques J. Biomed. Sci. (IF 9.0) Pub Date : 2024-06-01 Eric Dumonteil, Weihong Tu, Hans Desale, Kelly Goff, Preston Marx, Jaime Ortega-Lopez, Claudia Herrera
A vaccine against Trypanosoma cruzi, the agent of Chagas disease, would be an excellent additional tool for disease control. A recombinant vaccine based on Tc24 and TSA1 parasite antigens was found to be safe and immunogenic in naïve macaques. We used RNA-sequencing and performed a transcriptomic analysis of PBMC responses to vaccination of naïve macaques after each vaccine dose, to shed light on the
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Revolution in sepsis: a symptoms-based to a systems-based approach? J. Biomed. Sci. (IF 9.0) Pub Date : 2024-05-30 Geoffrey P. Dobson, Hayley L. Letson, Jodie L. Morris
Severe infection and sepsis are medical emergencies. High morbidity and mortality are linked to CNS dysfunction, excessive inflammation, immune compromise, coagulopathy and multiple organ dysfunction. Males appear to have a higher risk of mortality than females. Currently, there are few or no effective drug therapies to protect the brain, maintain the blood brain barrier, resolve excessive inflammation
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Development of a highly effective combination monoclonal antibody therapy against Herpes simplex virus J. Biomed. Sci. (IF 9.0) Pub Date : 2024-05-28 Narges Seyfizadeh, David Kalbermatter, Thomas Imhof, Moritz Ries, Christian Müller, Leonie Jenner, Elisabeth Blumenschein, Alexandra Yendrzheyevskiy, Frank Grün, Kevin Moog, Daniel Eckert, Ronja Engel, Philipp Diebolder, Mohamed Chami, Jürgen Krauss, Torsten Schaller, Michaela Arndt
Infections with Herpes simplex virus (HSV)-1 or -2 usually present as mild chronic recurrent disease, however in rare cases can result in life-threatening conditions with a large spectrum of pathology. Monoclonal antibody therapy has great potential especially to treat infections with virus resistant to standard therapies. HDIT101, a humanized IgG targeting HSV-1/2 gB was previously investigated in
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USP9X-mediated REV1 deubiquitination promotes lung cancer radioresistance via the action of REV1 as a Rad18 molecular scaffold for cystathionine γ-lyase J. Biomed. Sci. (IF 9.0) Pub Date : 2024-05-28 Yunshang Chen, Xue Feng, Zilong Wu, Yongqiang Yang, Xinrui Rao, Rui Meng, Sheng Zhang, Xiaorong Dong, Shuangbing Xu, Gang Wu, Xiaohua Jie
Radioresistance is a key clinical constraint on the efficacy of radiotherapy in lung cancer patients. REV1 DNA directed polymerase (REV1) plays an important role in repairing DNA damage and maintaining genomic stability. However, its role in the resistance to radiotherapy in lung cancer is not clear. This study aims to clarify the role of REV1 in lung cancer radioresistance, identify the intrinsic
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Tumor necrosis factor-inducible gene 6 protein and its derived peptide ameliorate liver fibrosis by repressing CD44 activation in mice with alcohol-related liver disease J. Biomed. Sci. (IF 9.0) Pub Date : 2024-05-24 Jinsol Han, Chanbin Lee, Hayeong Jeong, Seunghee Jeon, Myunggyo Lee, Haeseung Lee, Yung Hyun Choi, Youngmi Jung
Alcohol-related liver disease (ALD) is a major health concern worldwide, but effective therapeutics for ALD are still lacking. Tumor necrosis factor-inducible gene 6 protein (TSG-6), a cytokine released from mesenchymal stem cells, was shown to reduce liver fibrosis and promote successful liver repair in mice with chronically damaged livers. However, the effect of TSG-6 and the mechanism underlying
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Characterization of the genetic variation and evolutionary divergence of the CLEC18 family J. Biomed. Sci. (IF 9.0) Pub Date : 2024-05-20 Che-Mai Chang, Wei-Chiao Chang, Shie‐Liang Hsieh
The C-type lectin family 18 (CLEC18) with lipid and glycan binding capabilities is important to metabolic regulation and innate immune responses against viral infection. However, human CLEC18 comprises three paralogous genes with highly similar sequences, making it challenging to distinguish genetic variations, expression patterns, and biological functions of individual CLEC18 paralogs. Additionally
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Pivotal functions and impact of long con-coding RNAs on cellular processes and genome integrity J. Biomed. Sci. (IF 9.0) Pub Date : 2024-05-14 Siddhant Sharma, Aicha Asma Houfani, Leonard J. Foster
Recent advances in uncovering the mysteries of the human genome suggest that long non-coding RNAs (lncRNAs) are important regulatory components. Although lncRNAs are known to affect gene transcription, their mechanisms and biological implications are still unclear. Experimental research has shown that lncRNA synthesis, subcellular localization, and interactions with macromolecules like DNA, other RNAs
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Somatic PDGFRB activating variants promote smooth muscle cell phenotype modulation in intracranial fusiform aneurysm J. Biomed. Sci. (IF 9.0) Pub Date : 2024-05-13 Li Hao, Xiaolong Ya, Jiaye Wu, Chuming Tao, Ruochen Ma, Zhiyao Zheng, Siqi Mou, Yiming Ling, Yingxi Yang, Jiguang Wang, Yan Zhang, Qing Lin, Jizong Zhao
The fusiform aneurysm is a nonsaccular dilatation affecting the entire vessel wall over a short distance. Although PDGFRB somatic variants have been identified in fusiform intracranial aneurysms, the molecular and cellular mechanisms driving fusiform intracranial aneurysms due to PDGFRB somatic variants remain poorly understood. In this study, single-cell sequencing and immunofluorescence were employed
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A G-quadruplex-binding platinum complex induces cancer mitochondrial dysfunction through dual-targeting mitochondrial and nuclear G4 enriched genome J. Biomed. Sci. (IF 9.0) Pub Date : 2024-05-13 Keli Kuang, Chunyan Li, Fatlinda Maksut, Deepanjan Ghosh, Robin Vinck, Maolin Wang, Joël Poupon, Run Xiang, Wen Li, Fei Li, Zhu Wang, Junrong Du, Marie-Paule Teulade-Fichou, Gilles Gasser, Sophie Bombard, Tao Jia
G-quadruplex DNA (G4) is a non-canonical structure forming in guanine-rich regions, which play a vital role in cancer biology and are now being acknowledged in both nuclear and mitochondrial (mt) genome. However, the impact of G4-based targeted therapy on both nuclear and mt genome, affecting mt function and its underlying mechanisms remain largely unexplored. The mechanisms of action and therapeutic
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Excess glucose alone depress young mesenchymal stromal/stem cell osteogenesis and mitochondria activity within hours/days via NAD+/SIRT1 axis J. Biomed. Sci. (IF 9.0) Pub Date : 2024-05-13 B. Linju Yen, Li-Tzu Wang, Hsiu-Huang Wang, Chin-Pao Hung, Pei-Ju Hsu, Chia-Chi Chang, Chien-Yu Liao, Huey-Kang Sytwu, Men-Luh Yen
The impact of global overconsumption of simple sugars on bone health, which peaks in adolescence/early adulthood and correlates with osteoporosis (OP) and fracture risk decades, is unclear. Mesenchymal stromal/stem cells (MSCs) are the progenitors of osteoblasts/bone-forming cells, and known to decrease their osteogenic differentiation capacity with age. Alarmingly, while there is correlative evidence
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Contribution of extracellular vesicles for the pathogenesis of retinal diseases: shedding light on blood-retinal barrier dysfunction J. Biomed. Sci. (IF 9.0) Pub Date : 2024-05-10 Beatriz Martins, Maria Pires, António Francisco Ambrósio, Henrique Girão, Rosa Fernandes
Retinal degenerative diseases, including diabetic retinopathy (DR) and age-related macular degeneration (AMD), loom as threats to vision, causing detrimental effects on the structure and function of the retina. Central to understanding these diseases, is the compromised state of the blood-retinal barrier (BRB), an effective barrier that regulates the influx of immune and inflammatory components. Whether
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Exploiting urine-derived induced pluripotent stem cells for advancing precision medicine in cell therapy, disease modeling, and drug testing J. Biomed. Sci. (IF 9.0) Pub Date : 2024-05-09 Xiya Yin, Qingfeng Li, Yan Shu, Hongbing Wang, Biju Thomas, Joshua T. Maxwell, Yuanyuan Zhang
The field of regenerative medicine has witnessed remarkable advancements with the emergence of induced pluripotent stem cells (iPSCs) derived from a variety of sources. Among these, urine-derived induced pluripotent stem cells (u-iPSCs) have garnered substantial attention due to their non-invasive and patient-friendly acquisition method. This review manuscript delves into the potential and application
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Targeting cathepsin S promotes activation of OLF1-BDNF/TrkB axis to enhance cognitive function J. Biomed. Sci. (IF 9.0) Pub Date : 2024-05-09 Hao-Wei Lee, Szu-Jung Chen, Kuen-Jer Tsai, Kuei-Sen Hsu, Yi-Fan Chen, Chih-Hua Chang, Hsiao-Han Lin, Wen-Yun Hsueh, Hsing-Pang Hsieh, Yueh-Feng Lee, Huai-Chueh Chiang, Jang-Yang Chang
Cathepsin S (CTSS) is a cysteine protease that played diverse roles in immunity, tumor metastasis, aging and other pathological alterations. At the cellular level, increased CTSS levels have been associated with the secretion of pro-inflammatory cytokines and disrupted the homeostasis of Ca2+ flux. Once CTSS was suppressed, elevated levels of anti-inflammatory cytokines and changes of Ca2+ influx were
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Campylobacter jejuni virulence factors: update on emerging issues and trends J. Biomed. Sci. (IF 9.0) Pub Date : 2024-05-01 Alexandra Tikhomirova, Emmylee R. McNabb, Luca Petterlin, Georgia L. Bellamy, Kyaw H. Lin, Christopher A. Santoso, Ella S. Daye, Fatimah M. Alhaddad, Kah Peng Lee, Anna Roujeinikova
Campylobacter jejuni is a very common cause of gastroenteritis, and is frequently transmitted to humans through contaminated food products or water. Importantly, C. jejuni infections have a range of short- and long-term sequelae such as irritable bowel syndrome and Guillain Barre syndrome. C. jejuni triggers disease by employing a range of molecular strategies which enable it to colonise the gut, invade
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Membrane lipid remodeling eradicates Helicobacter pylori by manipulating the cholesteryl 6'-acylglucoside biosynthesis J. Biomed. Sci. (IF 9.0) Pub Date : 2024-04-29 Lih-Lih Ong, Hau-Ming Jan, Hong-Hanh Thi Le, Tsai-Chen Yang, Chou-Yu Kuo, Ai-Feng Feng, Kwok-Kong Tony Mong, Chun-Hung Lin
Helicobacter pylori, the main cause of various gastric diseases, infects approximately half of the human population. This pathogen is auxotrophic for cholesterol which it converts to various cholesteryl α-glucoside derivatives, including cholesteryl 6’-acyl α-glucoside (CAG). Since the related biosynthetic enzymes can be translocated to the host cells, the acyl chain of CAG likely comes from its precursor
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Dengue virus pathogenesis and host molecular machineries J. Biomed. Sci. (IF 9.0) Pub Date : 2024-04-22 Saumya Sinha, Kinjal Singh, Y. S. Ravi Kumar, Riya Roy, Sushant Phadnis, Varsha Meena, Sankar Bhattacharyya, Bhupendra Verma
Dengue viruses (DENV) are positive-stranded RNA viruses belonging to the Flaviviridae family. DENV is the causative agent of dengue, the most rapidly spreading viral disease transmitted by mosquitoes. Each year, millions of people contract the virus through bites from infected female mosquitoes of the Aedes species. In the majority of individuals, the infection is asymptomatic, and the immune system
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Targeting NLRP3 signaling reduces myocarditis-induced arrhythmogenesis and cardiac remodeling J. Biomed. Sci. (IF 9.0) Pub Date : 2024-04-22 Chye-Gen Chin, Yao-Chang Chen, Fong-Jhih Lin, Yung-Kuo Lin, Yen-Yu Lu, Tzu-Yu Cheng, Shih-Ann Chen, Yi-Jen Chen
Myocarditis substantially increases the risk of ventricular arrhythmia. Approximately 30% of all ventricular arrhythmia cases in patients with myocarditis originate from the right ventricular outflow tract (RVOT). However, the role of NLRP3 signaling in RVOT arrhythmogenesis remains unclear. Rats with myosin peptide–induced myocarditis (experimental group) were treated with an NLRP3 inhibitor (MCC950;
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T cell expressions of aberrant gene signatures and Co-inhibitory receptors (Co-IRs) as predictors of renal damage and lupus disease activity J. Biomed. Sci. (IF 9.0) Pub Date : 2024-04-22 Chin-Man Wang, Yeong-Jian Jan Wu, Jian-Wen Zheng, Li Yu Huang, Keng Poo Tan, Ji-Yih Chen
Systemic lupus erythematosus (SLE) is distinguished by an extensive range of clinical heterogeneity with unpredictable disease flares and organ damage. This research investigates the potential of aberrant signatures on T cell genes, soluble Co-IRs/ligands, and Co-IRs expression on T cells as biomarkers for lupus disease parameters. Comparative transcriptome profiling analysis of non-renal and end-stage
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Applications of peptides in nanosystems for diagnosing and managing bacterial sepsis J. Biomed. Sci. (IF 9.0) Pub Date : 2024-04-19 Mohammed A. Gafar, Calvin A. Omolo, Eman Elhassan, Usri H. Ibrahim, Thirumala Govender
Sepsis represents a critical medical condition stemming from an imbalanced host immune response to infections, which is linked to a significant burden of disease. Despite substantial efforts in laboratory and clinical research, sepsis remains a prominent contributor to mortality worldwide. Nanotechnology presents innovative opportunities for the advancement of sepsis diagnosis and treatment. Due to
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Enhancement of NETosis by ACE2-cross-reactive anti-SARS-CoV-2 RBD antibodies in patients with COVID-19 J. Biomed. Sci. (IF 9.0) Pub Date : 2024-04-18 Kun-Han Hsieh, Chiao-Hsuan Chao, Yi-Ling Cheng, Yen-Chung Lai, Yung-Chun Chuang, Jen-Ren Wang, Sui-Yuan Chang, Yuan-Pin Hung, Yi-Ming Arthur Chen, Wei-Lun Liu, Woei-Jer Chuang, Trai-Ming Yeh
High levels of neutrophil extracellular trap (NET) formation or NETosis and autoantibodies are related to poor prognosis and disease severity of COVID-19 patients. Human angiotensin-converting enzyme 2 (ACE2) cross-reactive anti-severe acute respiratory syndrome coronavirus 2 spike protein receptor-binding domain (SARS-CoV-2 RBD) antibodies (CR Abs) have been reported as one of the sources of anti-ACE2
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Attenuating mitochondrial dysfunction and morphological disruption with PT320 delays dopamine degeneration in MitoPark mice J. Biomed. Sci. (IF 9.0) Pub Date : 2024-04-17 Vicki Wang, Kuan-Yin Tseng, Tung-Tai Kuo, Eagle Yi-Kung Huang, Kuo-Lun Lan, Zi-Rong Chen, Kuo-Hsing Ma, Nigel H. Greig, Jin Jung, Ho-II Choi, Lars Olson, Barry J. Hoffer, Yuan-Hao Chen
Mitochondria are essential organelles involved in cellular energy production. Changes in mitochondrial function can lead to dysfunction and cell death in aging and age-related disorders. Recent research suggests that mitochondrial dysfunction is closely linked to neurodegenerative diseases. Glucagon-like peptide-1 receptor (GLP-1R) agonist has gained interest as a potential treatment for Parkinson's
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Longitudinal alterations in brain perfusion and vascular reactivity in the zQ175DN mouse model of Huntington’s disease J. Biomed. Sci. (IF 9.0) Pub Date : 2024-04-16 Tamara Vasilkovska, Somaie Salajeghe, Verdi Vanreusel, Johan Van Audekerke, Marlies Verschuuren, Lydiane Hirschler, Jan Warnking, Isabel Pintelon, Dorian Pustina, Roger Cachope, Ladislav Mrzljak, Ignacio Muñoz-Sanjuan, Emmanuel L. Barbier, Winnok H. De Vos, Annemie Van der Linden, Marleen Verhoye
Huntington’s disease (HD) is marked by a CAG-repeat expansion in the huntingtin gene that causes neuronal dysfunction and loss, affecting mainly the striatum and the cortex. Alterations in the neurovascular coupling system have been shown to lead to dysregulated energy supply to brain regions in several neurological diseases, including HD, which could potentially trigger the process of neurodegeneration
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Antimicrobial peptide thanatin fused endolysin PA90 (Tha-PA90) for the control of Acinetobacter baumannii infection in mouse model J. Biomed. Sci. (IF 9.0) Pub Date : 2024-04-15 Jeonghyun Lim, Heejoon Myung, Daejin Lim, Miryoung Song
This study addresses the urgent need for infection control agents driven by the rise of drug-resistant pathogens such as Acinetobacter baumannii. Our primary aim was to develop and assess a novel endolysin, Tha-PA90, designed to combat these challenges. Tha-PA90 incorporates an antimicrobial peptide (AMP) called thanatin at its N-terminus, enhancing bacterial outer membrane permeability and reducing
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Correction: A novel HIF1α-STIL-FOXM1 axis regulates tumor metastasis J. Biomed. Sci. (IF 9.0) Pub Date : 2024-04-09 Yi-Wei Wang, Shu-Chuan Chen, De-Leung Gu, Yi-Chen Yeh, Jhih-Jie Tsai, Kuo-Tai Yang, Yuh-Shan Jou, Teh-Ying Chou, Tang K. Tang
Correction: J Biomed Sci 29, 24 (2022) https://doi.org/10.1186/s12929-022-00807-0 After publication of this article [1], it was brought to our attention that the figure 6, supplementary figure s5, Supplementary Figure S8 need to be corrected. The Incorrect figure 6 is: The correct figure 6 is: The Incorrect supplementary figure s5 is: The correct supplementary figure s5 is: The Incorrect Supplementary
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Significance of hepatitis B virus capsid dephosphorylation via polymerase J. Biomed. Sci. (IF 9.0) Pub Date : 2024-04-01 Chih-Hsu Chang, Chiaho Shih
It is generally believed that hepatitis B virus (HBV) core protein (HBc) dephosphorylation (de-P) is important for viral DNA synthesis and virion secretion. HBV polymerase contains four domains for terminal protein, spacer, reverse transcriptase, and RNase H activities. HBV Polymerase mutants were transfected into HuH-7 cells and assayed for replication and HBc de-P by the Phos-tag gel analysis. Infection
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Association of TRAIL receptor with phosphatase SHP-1 enables repressing T cell receptor signaling and T cell activation through inactivating Lck J. Biomed. Sci. (IF 9.0) Pub Date : 2024-03-27 I-Tsu Chyuan, Hsiu-Jung Liao, Tse-Hua Tan, Huai-Chia Chuang, Yu-Chuan Chu, Meng-Hsun Pan, Chien-Sheng Wu, Ching-Liang Chu, Bor-Ching Sheu, Ping-Ning Hsu
T cell receptor (TCR) signaling and T cell activation are tightly regulated by gatekeepers to maintain immune tolerance and avoid autoimmunity. The TRAIL receptor (TRAIL-R) is a TNF-family death receptor that transduces apoptotic signals to induce cell death. Recent studies have indicated that TRAIL-R regulates T cell-mediated immune responses by directly inhibiting T cell activation without inducing
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Improving CRISPR–Cas9 directed faithful transgene integration outcomes by reducing unwanted random DNA integration J. Biomed. Sci. (IF 9.0) Pub Date : 2024-03-26 Rio Hermantara, Laura Richmond, Aqeel Faisal Taqi, Sabari Chilaka, Valentine Jeantet, Ileana Guerrini, Katherine West, Adam West
The field of genome editing has been revolutionized by the development of an easily programmable editing tool, the CRISPR–Cas9. Despite its promise, off-target activity of Cas9 posed a great disadvantage for genome editing purposes by causing DNA double strand breaks at off-target locations and causing unwanted editing outcomes. Furthermore, for gene integration applications, which introduce transgene