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Parallel Purification of Microscale Libraries via Automated Solid Phase Extraction SLAS Technol. (IF 2.7) Pub Date : 2024-02-28 Michael Wleklinski, Paige M. Carpenter, Kevin D. Dykstra, Anthony Donofrio, Timothy Nowak, Shane W. Krska, Ronald D. Ferguson
High-throughput experimentation (HTE) has become more widely utilized in drug discovery for rapid reaction optimization and generation of large synthetic compound arrays. While this has accelerated medicinal chemistry design, make, test (DMT) iterations, the bottleneck of purification persists, consuming time and resources. Herein we describe a general parallel purification approach based on solid
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Validating core therapeutic targets for osteoporosis treatment based on integrating network pharmacology and informatics SLAS Technol. (IF 2.7) Pub Date : 2024-02-14 Shiyang Weng, Huichao Fu, Shengxiang Xu, Jieruo Li
Our goal was to find metabolism-related lncRNAs that were associated with osteoporosis (OP) and construct a model for predicting OP progression using these lncRNAs. The GEO database was employed to obtain gene expression profiles. The WGCNA technique and differential expression analysis were used to identify hypoxia-related lncRNAs. A Lasso regression model was applied to select 25 hypoxia-related
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Employing synthetic biology to expand antibiotic discovery SLAS Technol. (IF 2.7) Pub Date : 2024-02-08 Greta D. Cook, Nikolas M. Stasulli
Antimicrobial-resistant (AMR) bacterial pathogens are a continually growing threat as our methods for combating these infections continue to be overcome by the evolution of resistance mechanisms. Recent therapeutic methods have not staved off the concern of AMR infections, so continued research focuses on new ways of identifying small molecules to treat AMR pathogens. While chemical modification of
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Engineering transcriptional regulation for cell-based therapies SLAS Technol. (IF 2.7) Pub Date : 2024-02-08 Matthias Recktenwald, Evan Hutt, Leah Davis, James MacAulay, Nichole M. Daringer, Peter A. Galie, Mary M. Staehle, Sebastián L. Vega
A major aim in the field of synthetic biology is developing tools capable of responding to user-defined inputs by activating therapeutically relevant cellular functions. Gene transcription and regulation in response to external stimuli are some of the most powerful and versatile of these cellular functions being explored. Motivated by the success of chimeric antigen receptor (CAR) T-cell therapies
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Magnetically Localized and wash-free Fluorescent Immuno-Assay: From a research platform (MLFIA) to a multiplexed POC system (MagIA) SLAS Technol. (IF 2.7) Pub Date : 2024-01-30 M. Fratzl, P. Bigotte, R. Gorbenkov, G. Goubet, P. Halfon, P. Kauffmann, D. Kirk, V. Masse, X. Payet-Burin, O. Ramel, S. Delshadi
Sexually transmitted infections (STI) remain one of the world's public health priorities: Nearly 400 million people are infected not only in emerging, but also in western countries. HIV, HBV and HCV share common infection pathways; thus these 3 diseases are recommended to be tested at the same time. However, this combined approach is currently mainly available in laboratories, and seldomly at the Point-of-care
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ANDeS: An Automated Nanoliter Droplet Selection and Collection Device SLAS Technol. (IF 2.7) Pub Date : 2023-11-18 Joaquín E. Urrutia Gómez, Razan El Khaled El Faraj, Moritz Braun, Pavel A. Levkin, Anna A. Popova
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A High-Throughput Quality Control Method for Assessing the Serial Dilution Performance of Dose–Response Plates with Acoustic Ejection Mass Spectrometry SLAS Technol. (IF 2.7) Pub Date : 2023-11-02 Mary Ashley Rimmer, Nathaniel Twarog, Yong Li, Anang A. Shelat, Zoran Rankovic, Lei Yang
This study aimed to develop a streamlined method for evaluating the dilution ratio of drug dose–response plates created by automated liquid handlers in the early stages of drug discovery. The quantitative techniques commonly used for this purpose have restrictions due to their limited linear dynamic range and inaccuracies in assessing serial dilution performance. To address this challenge, we describe
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Nanoparticles-based technologies for cholera detection and therapy SLAS Technol. (IF 2.7) Pub Date : 2023-11-03 Nathan Ho, Kaitlyn Tang, Vy Ngo, Isabella Livits, Alayne Morrel, Bari Noor, Kaylee Tseng, Eun Ji Chung
Cholera is a waterborne disease caused by Vibrio cholerae bacteria generally transmitted through contaminated food or water sources. Although it has been eradicated in most Western countries, cholera continues to be a highly transmitted and lethal disease in several African and Southeast Asian countries. Unfortunately, current diagnostic methods for cholera have challenges including high cost or delayed
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Automated Measurement of Transepithelial Electrical Resistance (TEER) in 96-Well Transwells using ECIS TEER96: Single and Multiple Time Point Assessments SLAS Technol. (IF 2.7) Pub Date : 2023-11-02 Jacob Schimetz, Pranav Shah, Charles Keese, Chris Dehnert, Michael Detweiler, Sam Michael, Catherine Toniatti-Yanulavich, Xin Xu, Elias Carvalho Padilha
Transepithelial electrical resistance (TEER) is a widely used technique for quantifying the permeability of epithelial and endothelial cell layers. However, traditional methods of measuring TEER are limited to single timepoint measurements and can subject cells to an altered environment during the measurement. Here, we assessed the validity of TEER measurements by the ECIS TEER96 device, which is designed
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State-of-the-art in engineering small molecule biosensors and their applications in metabolic engineering SLAS Technol. (IF 2.7) Pub Date : 2023-10-31 Patarasuda Chaisupa, R. Clay Wright
Genetically encoded biosensors are crucial for enhancing our understanding of how molecules regulate biological systems. Small molecule biosensors, in particular, help us understand the interaction between chemicals and biological processes. They also accelerate metabolic engineering by increasing screening throughput and eliminating the need for sample preparation through traditional chemical analysis
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Acoustic Droplet Ejection Facilitates Cell-based High-Throughput Screenings using Natural Products SLAS Technol. (IF 2.7) Pub Date : 2023-10-26 Thomas A. Mackenzie, José R. Tormo, Bastien Cautain, Germán Martínez, Isabel Sánchez, Olga Genilloud, Francisca Vicente, Maria C. Ramos
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Robotic cell processing facility for clinical research of retinal cell therapy SLAS Technol. (IF 2.7) Pub Date : 2023-10-29 Motoki Terada, Yu Kogawa, Yumiko Shibata, Michinori Kitagawa, Shinya Kato, Tomomitsu Iida, Tsuyoshi Yorimitsu, Akari Kato, Kenji Matsukuma, Tadao Maeda, Masayo Takahashi, Genki N. Kanda
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Point-of-care biomarker assay for rapid multiplexed detection of CRP and IP-10 SLAS Technol. (IF 2.7) Pub Date : 2023-10-14 Claire S. Wilson, Bhavya Vashi, Pavol Genzor, Melissa K. Gregory, Jason Yau, Lauren Wolfe, Michael J. Lochhead, Phil Papst, Kristen Pettrone, Paul W. Blair, Subramaniam Krishnan, Josh G. Chenoweth, Danielle V. Clark
Rapid and accurate measurements of immune protein markers are essential for diagnosis and treatment in all clinical settings. The recent pandemic has revealed a stark need for developing new tools and assays that could be rapidly used in diverse settings and provide useful information to clinicians. Here, we describe the development and test application of a novel one-step CRP/IP-10 duplex assay for
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Life sciences discovery and technology highlights SLAS Technol. (IF 2.7) Pub Date : 2023-10-12 Tal Murthy, Jamien Lim
Abstract not available
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TidyTron: Reducing lab waste using validated wash-and-reuse protocols for common plasticware in Opentrons OT-2 lab robots SLAS Technol. (IF 2.7) Pub Date : 2023-09-09 John A. Bryant, Cameron Longmire, Sriya Sridhar, Samuel Janousek, Mason Kellinger, R. Clay Wright
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COVID-19 diagnosis using clinical markers and multiple explainable artificial intelligence approaches: A case study from Ecuador SLAS Technol. (IF 2.7) Pub Date : 2023-09-09 Krishnaraj Chadaga, Srikanth Prabhu, Vivekananda Bhat, Niranjana Sampathila, Shashikiran Umakanth, Sudhakara Upadya P
The COVID-19 pandemic erupted at the beginning of 2020 and proved fatal, causing many casualties worldwide. Immediate and precise screening of affected patients is critical for disease control. COVID-19 is often confused with various other respiratory disorders since the symptoms are similar. As of today, the reverse transcription-polymerase chain reaction (RT-PCR) test is utilized for diagnosing COVID-19
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A Semi-Automated Pipeline for Quantifying Drusen-Like Deposits in Human Induced Pluripotent Stem Cell-Derived Retinal Pigmented Epithelial Cells SLAS Technol. (IF 2.7) Pub Date : 2023-08-30 Jenna Hall, Maciej Daniszewski, Shane Cheung, Kalyan Shobhana, Himeesh Kumar, Helena H Liang, Henry Beetham, Ellie Cho, Carla Abbott, Alex W Hewitt, Kaylene J Simpson, Robyn H Guymer, Daniel Paull, Alice Pébay, Grace E. Lidgerwood
Age-Related Macular Degeneration (AMD) is a highly prevalent form of retinal disease amongst Western communities over 50 years of age. A hallmark of AMD pathogenesis is the accumulation of drusen underneath the retinal pigment epithelium (RPE), a biological process also observable in vitro. The accumulation of drusen has been shown to predict the progression to advanced AMD, making accurate characterisation
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Multiplex digital microfluidics using serial controls and its applications in glucose sensing SLAS Technol. (IF 2.7) Pub Date : 2023-08-29 Xinyu Liu, Jinying Cai, Wenjia Wang, Yujuan Chai
Digital microfluidics (DMF) has found great applications in in vitro diagnostics (IVD). Compared to the microfabrication-based DMF, printed circuit board (PCB)-based DMF is more economical and compatible with existing IVD instruments. Despite that, current PCB-based DMF is oftentimes limited by the available droplets that can be controlled simultaneously, compromising their throughput and applications
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High-throughput differential scanning fluorimetry (DSF) and cellular thermal shift assays (CETSA): Shifting from manual to automated screening SLAS Technol. (IF 2.7) Pub Date : 2023-08-19 Catherine S. Hansel, Alice Lanne, Hannah Rowlands, Joseph Shaw, Matthew J. Collier, Helen Plant
Biophysical affinity screening is increasingly being adopted as a high-throughput hit finding technique in drug discovery. Automation is highly beneficial to high-throughput screening (HTS) since a large number of compounds need to be reproducibly tested against a biological target. Herein, we describe how we have automated two biophysical affinity screening methods that rely on a thermal shift in
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Automated Glycan-Bead Coupling for High Throughput, Highly Reproducible Anti-Glycan Antibody Analysis SLAS Technol. (IF 2.7) Pub Date : 2023-08-16 Antonia Katharina Hefermehl, Sanne Maria Mathias Hensen, Carina Versantvoort, Andrée Rothermel, Uğur Şahin
Automation of diagnostic assays generally aims to increase reproducibility and throughput while decreasing human errors and hands-on time. Here, we introduce a protocol for the automated chemical conjugation of glycans to color-coded magnetic beads using the KingFisher Flex magnetic particle processor. The resulting glycan-coupled magnetic beads allow the detection of anti-glycan antibodies of different
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Automated cell culture system for the production of cell aggregates with growth plate-like structure from induced pluripotent stem cells. SLAS Technol. (IF 2.7) Pub Date : 2023-08-09 Akira Ohta, Shunsuke Kawai, Yann Pretemer, Megumi Nishio, Sanae Nagata, Hiromitsu Fuse, Yukiko Yamagishi, Junya Toguchida
Programmable liquid handling devices for cell culture systems have dramatically enhanced scalability and reproducibility. We previously reported a protocol to produce cell aggregates demonstrating growth plate-like structures containing hypertrophic chondrocytes from human induced pluripotent stem cells (hiPSCs). To apply this protocol to large-scale drug screening for growth plate-related diseases
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Identification of m6A-related lncRNAs-based signature for predicting the prognosis of patients with skin cutaneous melanoma SLAS Technol. (IF 2.7) Pub Date : 2023-08-02 Wentao Lin, Zhou-yong Tan, Xi-chi Fang
Background : Skin cutaneous melanoma (SKCM) is one of the fastest developing malignancies with strong aggressive ability and no proper curative treatments. Numerous studies illustrated the importance of N6-methyladenosine (m6A) RNA modification to tumorigenesis. The aim of this study was to identify novel prognostic signature by using m6A-related lncRNAs, thus to improve the survival for SKCM patients
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The Tecan SiLA2 SDK: A royalty-free, open-source framework to develop SiLA2 servers and clients SLAS Technol. (IF 2.7) Pub Date : 2023-07-20 Georg Hinkel, Jörg Kunert, Jason Meredith
Many devices in a laboratory only have proprietary interfaces. Integrating them into an automation platform such as a Robotic Liquid Handler (RLH) thus usually requires upfront technical configuration and often dedicated software development. In this paper, we present the Tecan SiLA2 SDK, a BSD-3 licensed, royalty-free open-source framework on the.NET platform that makes it very easy for developers
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Improving protein therapeutic development through cloud-based data integration SLAS Technol. (IF 2.7) Pub Date : 2023-07-16 George A. Van Den Driessche, Devin Bailey, Evan O. Anderson, Michael A Tarselli, Len Blackwell
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Automated human induced pluripotent stem cell colony segmentation for use in cell culture automation applications SLAS Technol. (IF 2.7) Pub Date : 2023-07-15 Kimerly A. Powell, Laura R. Bohrer, Nicholas E. Stone, Bradley Hittle, Kristin R. Anfinson, Viviane Luangphakdy, George Muschler, Robert F. Mullins, Edwin M. Stone, Budd A. Tucker
Human induced pluripotent stem cells (hiPSCs) have demonstrated great promise for a variety of applications that include cell therapy and regenerative medicine. Production of clinical grade hiPSCs requires reproducible manufacturing methods with stringent quality-controls such as those provided by image-controlled robotic processing systems. In this paper we present an automated image analysis method
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A rapid, high-throughput, viral infectivity assay using automated brightfield microscopy with machine learning SLAS Technol. (IF 2.7) Pub Date : 2023-07-13 Rupert Dodkins, John R. Delaney, Tess Overton, Frank Scholle, Alba Frias-De-Diego, Elisa Crisci, Nafisa Huq, Ingo Jordan, Jason T. Kimata, Teresa Findley, Ilya G. Goldberg
Infectivity assays are essential for the development of viral vaccines, antiviral therapies, and the manufacture of biologicals. Traditionally, these assays take 2–7 days and require several manual processing steps after infection. We describe an automated viral infectivity assay (AVIATM), using convolutional neural networks (CNNs) and high-throughput brightfield microscopy on 96-well plates that can
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Capillary electrophoresis methods for determining the IVT mRNA critical quality attributes of size and purity SLAS Technol. (IF 2.7) Pub Date : 2023-07-02 Denise A Warzak, Whitney A Pike, Kyle D Luttgeharm
One result of the Covid-19 pandemic has been an increased awareness of IVT mRNA vaccines and the speed at which they can be produced for disease outbreaks. Currently the only approved IVT mRNA therapeutics are the Covid-19 vaccines, however IVT mRNA is being investigated for other non-Covid prophylactic vaccines, therapeutics, and therapeutic vaccines. IVT mRNAs can range from less than 100 nt in length
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Development of mechanosensitive synthetic cells for biomedical applications SLAS Technol. (IF 2.7) Pub Date : 2023-06-28 Yen-Yu Hsu, Sung-Won Hwang, Samuel J. Chen, Eben Alsberg, Allen P. Liu
The ability of cells to sense and respond to their physical environment plays a fundamental role in a broad spectrum of biological processes. As one of the most essential molecular force sensors and transducers found in cell membranes, mechanosensitive (MS) ion channels can convert mechanical inputs into biochemical or electrical signals to mediate a variety of sensations. The bottom-up construction
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Greening automation: Wash and Re-use of disposable 384-well liquid handling tips to enable sustainable high-throughput vaccine development SLAS Technol. (IF 2.7) Pub Date : 2023-06-14 Brittney Pedrazzi, Aleksandr Treyer, Rachael Cohen, Amy Bowman, Jillian Acevedo-Skrip, Kristine Kearns, David Westover, John W. Loughney
Laboratory automation uses large amounts of plastic consumables, generating substantial single-use plastic waste. Automated ELISAs are an indispensable analytical tool in vaccine formulation and process development. Current workflows, however, rely on disposable liquid handling tips. In progress toward sustainability, we developed workflows for washing 384-well format liquid handling tips, using nontoxic
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Sample–to-answer sensing technologies for nucleic acid preparation and detection in the field SLAS Technol. (IF 2.7) Pub Date : 2023-06-09 Chia-Wei Liu, Hideaki Tsutsui
Efficient sample preparation and accurate disease diagnosis under field conditions are of great importance for the early intervention of diseases in humans, animals, and plants. However, in-field preparation of high-quality nucleic acids from various specimens for downstream analyses, such as amplification and sequencing, is challenging. Thus, developing and adapting sample lysis and nucleic acid extraction
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Bioprinting the future SLAS Technol. (IF 2.7) Pub Date : 2023-05-29 Roman Voronov, Murat Guvendiren
Abstract not available
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Recent Advances of Droplet-based Microfluidics for Engineering Artificial Cells SLAS Technol. (IF 2.7) Pub Date : 2023-05-26 Samantha Fasciano, Shue Wang
Artificial cells, synthetic cells, or minimal cells are microengineered cell-like structures that mimic the biological functions of cells. Artificial cells are typically biological or polymeric membranes where biologically active components, including proteins, genes, and enzymes, are encapsulated. The goal of engineering artificial cells is to build a living cell with the least amount of parts and
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A sticky-end probe biosensor for homogeneous detection of transcription factor binding activity SLAS Technol. (IF 2.7) Pub Date : 2023-05-21 Joao Paulo Pera Mendes, Ninghao Zhu, Pak Kin Wong
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Carboxymethyl cellulose-agarose-gelatin: A thermoresponsive triad bioink composition to fabricate volumetric soft tissue constructs SLAS Technol. (IF 2.7) Pub Date : 2023-05-04 Muthu Parkkavi Sekar, Harshavardhan Budharaju, Swaminathan Sethuraman, Dhakshinamoorthy Sundaramurthi
Polysaccharide based hydrogels have been predominantly utilized as ink materials for 3D bioprinting due to biocompatibility and cell responsive features. However, most hydrogels require extensive crosslinking due to poor mechanical properties leading to limited printability. To improve printability without using cytotoxic crosslinkers, thermoresponsive bioinks could be developed. Agarose is a thermoresponsive
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Biomimetic scaffolds using triply periodic minimal surface-based porous structures for biomedical applications SLAS Technol. (IF 2.7) Pub Date : 2023-04-29 Raffaele Pugliese, Serena Graziosi
The design of biomimetic porous scaffolds has been gaining attention in the biomedical sector lately. Shells, marine sponges, shark teeth, cancellous bone, sea urchin spine, and the armadillo armor structure are examples of biological systems that have already been studied to drive the design of innovative, porous, and multifunctional structures. Among these, triply periodic minimal surfaces (TPMSs)
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Development and validation of an automated assay for anti-drug-antibodies in rat serum SLAS Technol. (IF 2.7) Pub Date : 2023-04-28 Kristy A. Terrell, Gregory D. Sempowski, Andrew N. Macintyre
The potential immunogenicity of therapeutic human and humanized monoclonal antibodies (mAb) is a significant concern, and so preclinical testing of therapeutic mAbs routinely includes assessment of anti-drug antibody (ADA) induction. Here, we report the development of automated screening and confirmatory bridging ELISAs for the detection of rat antibodies against DH1042, an engineered human mAb for
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Screening Station, a novel laboratory automation system for physiologically relevant cell-based assays SLAS Technol. (IF 2.7) Pub Date : 2023-04-28 Ichiji Namatame, Kana Ishii, Takashi Shin, Daisuke Shimojo, Yukiko Yamagishi, Hidemitsu Asano, Yuuki Kishimoto, Hiromitsu Fuse, Yohei Nishi, Hidetoshi Sakurai, Tatsutoshi Nakahata, Haruna Sasaki-Iwaoka
Due to their physiological relevance, cell-based assays using human-induced pluripotent stem cell (iPSC)-derived cells are a promising in vitro pharmacological evaluation system for drug candidates. However, cell-based assays involve complex processes such as long-term culture, real-time and continuous observation of living cells, and detection of many cellular events. Automating multi-sample processing
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Life sciences discovery and technology highlights SLAS Technol. (IF 2.7) Pub Date : 2023-04-26 Tal Murthy, Jamien Lim
Abstract not available
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Additive manufacturing of peripheral nerve conduits – Fabrication methods, design considerations and clinical challenges SLAS Technol. (IF 2.7) Pub Date : 2023-04-05 Allen Zennifer, Madhumithra Thangadurai, Dhakshinamoorthy Sundaramurthi, Swaminathan Sethuraman
Tissue-engineered nerve guidance conduits (NGCs) are a viable clinical alternative to autografts and allografts and have been widely used to treat peripheral nerve injuries (PNIs). Although these NGCs are successful to some extent, they cannot aid in native regeneration by improving native-equivalent neural innervation or regrowth. Further, NGCs exhibit longer recovery period and high cost limiting
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Salivary gland regeneration: from salivary gland stem cells to three-dimensional bioprinting SLAS Technol. (IF 2.7) Pub Date : 2023-04-03 Toan V. Phan, Yamin Oo, Khurshid Ahmed, Teerapat Rodboon, Vinicius Rosa, Supansa Yodmuang, Joao N. Ferreira
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Advances in tissue engineering of cancer microenvironment-from three-dimensional culture to three-dimensional printing SLAS Technol. (IF 2.7) Pub Date : 2023-04-03 Joana Rita Oliveira Faria Marques, Patricia González-Alva, Ruby Yu-Tong Lin, Beatriz Ferreira Fernandes, Akhilanand Chaurasia, Nileshkumar Dubey
Cancer treatment development is a complex process, with tumor heterogeneity and inter-patient variations limiting the success of therapeutic intervention. Traditional two-dimensional cell culture has been used to study cancer metabolism, but it fails to capture physiologically relevant cell-cell and cell-environment interactions required to mimic tumor-specific architecture. Over the past three decades
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Biomedical applications of nanodiamonds: From drug-delivery to diagnostics SLAS Technol. (IF 2.7) Pub Date : 2023-04-01 Jingru Xu, Edward Kai-Hua Chow
Advances in nanotechnology have great potential to address many unmet clinical and biomedical needs. Nanodiamonds, as a class of carbon nanoparticles with unique properties, may be useful towards a versatile range of biomedical applications from drug delivery to diagnostics. This review describes how these properties of nanodiamonds facilitate their application in different fields of biomedicine, including
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High-throughput cell spheroid production and assembly analysis by microfluidics and deep learning SLAS Technol. (IF 2.7) Pub Date : 2023-03-28 Martin Trossbach, Emma Åkerlund, Krzysztof Langer, Brinton Seashore-Ludlow, Haakan N. Joensson
3D cell culture models are important tools in translational research but have been out of reach for high-throughput screening due to complexity, requirement of large cell numbers and inadequate standardization. Microfluidics and culture model miniaturization technologies could overcome these challenges. Here, we present a high-throughput workflow to produce and characterize the formation of miniaturized
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SAGAS: Simulated annealing and greedy algorithm scheduler for laboratory automation SLAS Technol. (IF 2.7) Pub Date : 2023-03-28 Yuya Arai, Ko Takahashi, Takaaki Horinouchi, Koichi Takahashi, Haruka Ozaki
During laboratory automation of life science experiments, coordinating specialized instruments and human experimenters for various experimental procedures is important to minimize the execution time. In particular, the scheduling of life science experiments requires the consideration of time constraints by mutual boundaries (TCMB) and can be formulated as the “scheduling for laboratory automation in
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Plant molecular farming-derived epidermal growth factor revolutionizes hydrogels for improving glandular epithelial organoid biofabrication SLAS Technol. (IF 2.7) Pub Date : 2023-03-24 Toan V. Phan, Yamin Oo, Teerapat Rodboon, Truc T. Nguyen, Ladawan Sariya, Risa Chaisuparat, Waranyoo Phoolcharoen, Supansa Yodmuang, Joao N. Ferreira
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Automated sample preparation of protein solid dosage forms: Novel application for the tablet processing workstation SLAS Technol. (IF 2.7) Pub Date : 2023-03-03 Ivana Fileš, Vincent Andersson
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Life sciences discovery and technology highlights SLAS Technol. (IF 2.7) Pub Date : 2023-02-28 Jamien Lim, Tal Murthy
Abstract not available
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Bioprinting the future using light: A review on photocrosslinking reactions, photoreactive groups, and photoinitiators SLAS Technol. (IF 2.7) Pub Date : 2023-02-19 Kamil Elkhoury, Julio Zuazola, Sanjairaj Vijayavenkataraman
Light-based bioprinting is a type of additive manufacturing technologies that uses light to control the formation of biomaterials, tissues, and organs. It has the potential to revolutionize the adopted approach in tissue engineering and regenerative medicine by allowing the creation of functional tissues and organs with high precision and control. The main chemical components of light-based bioprinting
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In vitro delivery of mTOR inhibitors by kidney-targeted micelles for autosomal dominant polycystic kidney disease SLAS Technol. (IF 2.7) Pub Date : 2023-02-19 Alysia Cox, Madelynn Tung, Hui Li, Kenneth R. Hallows, Eun Ji Chung
Autosomal dominant polycystic kidney disease (ADPKD) is the most common genetic kidney disease and is characterized by the formation of renal cysts and the eventual development of end-stage kidney disease. One approach to treating ADPKD is through inhibition of the mammalian target of rapamycin (mTOR) pathway, which has been implicated in cell overproliferation, contributing to renal cyst expansion
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Quantitative assessment of automated purification and concentration of E. coli bacteria SLAS Technol. (IF 2.7) Pub Date : 2023-02-18 Nina Sara Fraticelli Guzmán, Mohamed W. Badawy, Max A. Stockslager, Michael L. Farrell, Caitlin van Zyl, Seth Stewart, David L. Hu, Craig R. Forest
Automated methods for rapidly purifying and concentrating bacteria from environmental interferents are needed in next-generation applications for anything from water purification to biological weapons detection. Though previous work has been performed by other researchers in this area, there is still a need to create an automated system that can both purify and concentrate target pathogens in a timely
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Recent progress of 4D printing in cancer therapeutics studies SLAS Technol. (IF 2.7) Pub Date : 2023-02-17 Atchara Chinnakorn, Wiwat Nuansing, Mahdi Bodaghi, Bernard Rolfe, Ali Zolfagharian
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Incorporation of automated buffer exchange empowers high-throughput protein and plasmid purification for downstream uses SLAS Technol. (IF 2.7) Pub Date : 2023-02-01 Patrick A. Kates, Jordan N. Cook, Ryan Ghan, Huey J. Nguyen, Pongkwan Sitasuwan, L. Andrew Lee
The continued acceleration of time-to-market product development and rising demand for biotherapeutics have hastened the need for higher throughput within the biopharmaceutical industry. Automated liquid handlers (ALH) are increasingly popular due to flexible programming that enables processing of multiple samples with an array of functions. This flexibility is useful in streamlining research that
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Mera: A scalable high throughput automated micro-physiological system SLAS Technol. (IF 2.7) Pub Date : 2023-01-26 Finola E. Cliffe, Conor Madden, Patrick Costello, Shane Devitt, Sumir Ramesh Mukkunda, Bhairavi Bengaluru Keshava, Howard O. Fearnhead, Aiste Vitkauskaite, Mahshid H. Dehkordi, Walter Chingwaru, Milosz Przyjalgowski, Natalia Rebrova, Mark Lyons
There is an urgent need for scalable Microphysiological Systems (MPS's)1 that can better predict drug efficacy and toxicity at the preclinical screening stage. Here we present Mera, an automated, modular and scalable system for culturing and assaying microtissues with interconnected fluidics, inbuilt environmental control and automated image capture. The system presented has multiple possible fluidics
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Development of a novel, automated, robotic system for rapid, high-throughput, parallel, solid-phase peptide synthesis SLAS Technol. (IF 2.7) Pub Date : 2023-01-14 Krisztina Kiss, Soma Ránky, Zsuzsanna Gyulai, László Molnár
The development of peptide-based pharmaceutics is a hot topic in the pharmaceutical industry and in basic research. However, from the research and development perspective there is an unmet need for new, alternative, solid-phase peptide synthesizers that are highly efficient, automated, robust, able to synthetize peptides in parallel, inexpensive (to obtain and operate), have potential to be scaled
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Towards robotic laboratory automation Plug & play: Survey and concept proposal on teaching-free robot integration with the lapp digital twin SLAS Technol. (IF 2.7) Pub Date : 2023-01-13 Ádám Wolf, Stefan Romeder-Finger, Károly Széll, Péter Galambos
The Laboratory Automation Plug & Play (LAPP) framework is an over-arching reference architecture concept for the integration of robots in life science laboratories. The plug & play nature lies in the fact that manual configuration is not required, including the teaching of the robots. In this paper a digital twin (DT) based concept is proposed that outlines the types of information that must be provided
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Ultrasound frequency sonication facilitates high-throughput and uniform dissociation of cellular aggregates and tissues SLAS Technol. (IF 2.7) Pub Date : 2023-01-12 E. Celeste Welch, Katherine Chaltas, Anubhav Tripathi
A sample preparation step involving dissociation of tissues into their component cells is often required to conduct analysis of nucleic acids and other constituents from tissue samples. Frequently, the extracellular matrix and cell-cell adhesions are disrupted via treatment with a chemical dissociating reagent or various mechanical forces. In this work, a new, high-throughput, multiplexed method of
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The 2023 SLAS technology ten: Translating life sciences innovation SLAS Technol. (IF 2.7) Pub Date : 2022-12-26 Edward Kai-Hua Chow
Abstract not available
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Life sciences discovery and technology highlights SLAS Technol. (IF 2.7) Pub Date : 2022-12-09 Tal Murthy, Jamien Lim
Abstract not available
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Automation of yeast spot assays using an affordable liquid handling robot SLAS Technol. (IF 2.7) Pub Date : 2022-12-09 Shodai Taguchi, Yasuyuki Suda, Kenji Irie, Haruka Ozaki
The spot assay of the budding yeast Saccharomyces cerevisiae is an experimental method that is used to evaluate the effect of genotypes, medium conditions, and environmental stresses on cell growth and survival. Automation of the spot assay experiments from preparing a dilution series to spotting to observing spots continuously has been implemented based on large laboratory automation devices and robots