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Regulation of Blood Group Expression: Another Layer of Complexity to Consider. Transfus. Med. Hemother. (IF 1.9) Pub Date : 2024-07-02 Christoph Gassner,Martin L Olsson
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Regulation of the Lewis Blood Group Antigen Expression: A Literature Review Supplemented with Computational Analysis. Transfus. Med. Hemother. (IF 1.9) Pub Date : 2024-06-19 Martin Wipplinger,Sylvia Mink,Maike Bublitz,Christoph Gassner
Background The Lewis (Le) blood group system, unlike most other blood groups, is not defined by antigens produced internally to the erythrocytes and their precursors but rather by glycan antigens adsorbed on to the erythrocyte membrane from the plasma. These oligosaccharides are synthesized by the two fucosyltransferases FUT2 and FUT3 mainly in epithelial cells of the digestive tract and transferred
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Role of MiRNA in the Regulation of Blood Group Expression. Transfus. Med. Hemother. (IF 1.9) Pub Date : 2024-06-04 Romy Kronstein-Wiedemann,Stephan R Künzel,Jessica Thiel,Torsten Tonn
Background MicroRNAs (miRNAs) are small, endogenous non-coding RNA molecules that inhibit gene expression through either destabilization of the target mRNA or translational repression. MiRNAs recognize target sites, most commonly found in the 3'-untranslated regions of cognate mRNAs. This review aims to provide a state-of-the-art overview of the role of miRNAs in the regulation of major blood group
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HLA in Transplantation: Challenges and Perspectives. Transfus. Med. Hemother. (IF 1.9) Pub Date : 2024-06-03 Nils Lachmann,Axel Pruß
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A Bioinformatically Initiated Approach to Evaluate GATA1 Regulatory Regions in Samples with Weak D, Del, or D- Phenotypes Despite Normal RHD Exons. Transfus. Med. Hemother. (IF 1.9) Pub Date : 2024-05-10 Eunike C McGowan,Ping Chun Wu,Åsa Hellberg,Genghis H Lopez,Catherine A Hyland,Martin L Olsson
Introduction With over 360 blood group antigens in systems recognized, there are antigens, such as RhD, which demonstrate a quantitative reduction in antigen expression due to nucleotide variants in the non-coding region of the gene that result in aberrant splicing or a regulatory mechanism. This study aimed to evaluate bioinformatically predicted GATA1-binding regulatory motifs in the RHD gene for
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A Short History of B-Cell HLA Epitopes. Transfus. Med. Hemother. (IF 1.9) Pub Date : 2024-04-22 Ilias Doxiadis,Henry Loeffler-Wirth,Nils Lachmann,Claudia Lehmann
Background HLA epitopes are currently in the focus of transplantation immunogenetics. The main reason is the complexity of the HLA system with >38,000 alleles, the number of which increases steadily. These alleles are determined by the current state-of-the art typing methods like second- and third-generation sequencing. Screening for HLA antibodies is hampered by the lack of specific target beads with
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Successful Desensitization with Imlifidase and Daratumumab in a Highly Immunized, Crossmatch Positive, Blood Group-Incompatible Living-Donor Re-Transplant Recipient with Systemic Lupus Erythematosus and Antiphospholipid Syndrome. Transfus. Med. Hemother. (IF 1.9) Pub Date : 2024-04-22 Eva Schrezenmeier,Mira Choi,Brigitta Globke,Thomas Dörner,Alexandra Leimbach,Bilgin Osmanodja,Alexander Schramm,Kerstin Amann,Kai-Uwe Eckardt,Klemens Budde,Robert Öllinger,Nils Lachmann,Fabian Halleck
Introduction The transplantation of highly sensitized patients remains a major obstacle. Immunized patients wait longer for a transplant if not prioritized, and if transplanted, their transplant outcome is worse. Case Presentation We report a successful AB0- and HLA-incompatible living donor kidney transplantation in a 35-year-old female patient with systemic lupus erythematosus (SLE) and antiphospholipid
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Experimental Data on PIRCHE and T-Cell Reactivity: HLA-DPB1-Derived Peptides Identified by PIRCHE-I Show Binding to HLA-A*02:01 in vitro and T-Cell Activation in vivo. Transfus. Med. Hemother. (IF 1.9) Pub Date : 2024-04-02 Emma T M Peereboom,Anna E Maranus,Laura M Timmerman,Kirsten Geneugelijk,Eric Spierings
Introduction Human leukocyte antigen (HLA)-DPB1 mismatches during hematopoietic stem cell transplantation (HSCT) with an unrelated donor result in an increased risk for the development of graft-versus-host disease (GvHD). The number of CD8+ T-cell epitopes available for indirect allorecognition as predicted by the PIRCHE algorithm has been shown to be associated with GvHD development. As a proof of
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Review of ABO Expression and Variations based on Transcriptional Regulation of the ABO Blood Group Gene. Transfus. Med. Hemother. (IF 1.9) Pub Date : 2024-03-12 Kenichi Ogasawara,Rie Sano,Yoshihiko Kominato
Background and Summary We review the transcriptional regulation of ABO expression and discuss variants in the promoter and erythroid cell-specific regulatory region in individuals with weak ABO phenotypes such as Bm, Am, B3, and A3. We also review the molecular mechanisms responsible for variations in ABO expression in development and disease including the cell type-specific expression of ABO during
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Positive Long-Term Outcome of Kidney Allocation via Acceptable Mismatch Program in Highly Sensitized Patients. Transfus. Med. Hemother. (IF 1.9) Pub Date : 2024-02-28 Yara Strehler,Nils Lachmann,Matthias Niemann,Fabian Halleck,Klemens Budde,Axel Pruß
Introduction Eurotransplant established the acceptable mismatch (AM) program to facilitate timely kidney transplantations of highly sensitized patients, but long-term granular clinical and immunological outcomes regarding overall graft survival and de novo DSA (dnDSA) formation are still intensively researched. The right choice of induction therapy in patients with differing immunological risk is not
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Growth and Distribution of Bacteria in Contaminated Whole Blood and Derived Blood Components. Transfus. Med. Hemother. (IF 1.9) Pub Date : 2024-02-21 Ute Gravemann,Wiebke Handke,Torsten J Schulze,Axel Seltsam
Introduction Bacterial contamination of blood products presumably occurs mainly during blood collection, starting from low initial concentrations of 10-100 colony-forming units (CFUs) per bag. As little is known about bacterial growth behavior and distribution in stored whole blood (WB) and WB-derived blood products, this study aims to provide data on this subject. Methods WB units were inoculated
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Increase of Phosphoprotein Expressions in Amotosalen/UVA-Treated Platelet Concentrates. Transfus. Med. Hemother. (IF 1.9) Pub Date : 2024-02-09 Charlotte Muret,David Crettaz,Lorenzo Alberio,Michel Prudent
Background Pathogen inactivation treatment (PIT) has been shown to alter platelet function, phenotype, morphology and to induce a faster aging of platelet concentrates (PCs). Key pieces of information are still missing to understand the impacts of PITs at the cellular level. Objectives This study investigated the impact of amotosalen/UVA on PCs, from a post-translational modifications (PTM) point of
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A Call to Reinvent Platelet Products. Transfus. Med. Hemother. (IF 1.9) Pub Date : 2024-02-07 Stefan Handtke,Thomas Thiele
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In vitro Evaluation of DINCH-Plasticized Blood Bags for Red Blood Cell Storage with CPDA-1 Anticoagulant. Transfus. Med. Hemother. (IF 1.9) Pub Date : 2024-01-04 Hyungsuk Kim,Kyunghoon Lee,Soo Hyun Seo,Yun Ji Hong,Sang Mee Hwang,Jeong Su Park,Kyoung Un Park,Junghan Song
Introduction Di(2-ethylhexyl) phthalate (DEHP) is a plasticizer commonly used in blood bags. Despite its protective effects on red blood cell (RBC) storage, concerns about its reproductive toxicity exist. This study investigated the in vitro quality of RBC concentrates stored in bags using di(isononyl) cyclohexane-1,2-dicarboxylate (DINCH) as an alternative plasticizer. Methods Using a pool-and-split
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Congratulation, Appraisal, and Comment on the 25 Years Anniversary of Serious Hazards of Blood Transfusion. Transfus. Med. Hemother. (IF 1.9) Pub Date : 2023-11-27 Thomas Frietsch,Maria B Rondinelli,Jerrold H Levy
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In vitro Hemostatic Functions of Cold-Stored Platelets. Transfus. Med. Hemother. (IF 1.9) Pub Date : 2023-11-16 Johanna Kirschall,Günalp Uzun,Tamam Bakchoul,Irene Marini
Background Transfusion of platelets is a life-saving medical strategy used worldwide to treat patients with thrombocytopenia as well as platelet function disorders. Summary Until the end of 1960s, platelets were stored in the cold because of their superior hemostatic functionality. Cold storage of platelets was then abandoned due to better posttransfusion recovery and survival of room temperature (RT)-stored
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CRISPR/Cas9-Editing K562 Cell Line as a Potential Tool in Transfusion Applications: Knockout of Vel Antigen Gene. Transfus. Med. Hemother. (IF 1.9) Pub Date : 2023-11-02 Jiaxuan Yang,Aijing Li,Minghao Li,Shulin Ruan,Luyi Ye
Introduction The Vel- phenotype is a rare blood group, and it is challenging for identifying this phenotype due to limited available reagents. Moreover, there are relatively few studies on genomic editing of erythroid antigens and generation of knockout (KO) cell lines at present. Methods To identify the high-efficiency small-guiding RNA (sgRNA) sequence, candidate sgRNAs were transfected into HEK
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Evorpacept-Induced Interference and Application of a Novel Mitigation Agent, Evo-NR, in Pretransfusion Testing. Transfus. Med. Hemother. (IF 1.9) Pub Date : 2023-10-25 Eungjun Yoon,Tae Yeul Kim,Hyungsuk Kim,Duck Cho
Introduction Evorpacept is a CD47-blocking agent currently being developed for the treatment of various cancers. Interference by evorpacept in pretransfusion compatibility testing has been reported at limited plasma concentrations. Although various mitigation strategies have been proposed, none are practical. This in vitro study assessed evorpacept-induced interference at extended concentrations and
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Patient Blood Management: We Still Have Work to Do. Transfus. Med. Hemother. (IF 1.9) Pub Date : 2023-10-25 Patrick Meybohm,Lotta Hof,Suma Choorapoikayil,Kai Zacharowski
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Indocyanine Green-Labeled Platelets for Survival and Recovery Studies. Transfus. Med. Hemother. (IF 1.9) Pub Date : 2023-10-16 Johannes-Moritz von Behren,Jan Wesche,Andreas Greinacher,Konstanze Aurich
Introduction Before being implemented in daily clinical routine, new production strategies for platelet concentrates (PCs) must be validated for their efficacy. Besides in vitro testing, the establishment of new methods requires the labeling of platelets for in vivo studies of platelets' survival and recovery. Indocyanine green (ICG) is a Food and Drug Administration-approved near-infrared (NIR) fluorescent
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A German-Wide Systematic Study on Mobilization and Collection of Hematopoietic Stem Cells in Poor Mobilizer Patients with Multiple Myeloma prior to Autologous Stem Cell Transplantation. Transfus. Med. Hemother. (IF 1.9) Pub Date : 2023-10-16 Max Bittrich,Katharina Kriegsmann,Carola Tietze-Stolley,Kamran Movassaghi,Matthias Grube,Vladan Vucinic,Daniela Wehler,Andreas Burchert,Martin Schmidt-Hieber,Andreas Rank,Heinz A Dürk,Bernd Metzner,Christoph Kimmich,Marcus Hentrich,Christian Kunz,Frank Hartmann,Cyrus Khandanpour,Maike de Wit,Udo Holtick,Michael Kiehl,Andrea Stoltefuß,Alexander Kiani,Ralph Naumann,Christian W Scholz,Hans-Joachim Tischler
Introduction In patients with a clinical indication for autologous hematopoietic stem cell transplantation (ASCT), sufficient mobilization of CD34+ precursor cells into peripheral blood is essential to ensure adequate hematopoietic stem cell (HSC) collection prior to intensive therapy. However, with standard granulocyte-colony stimulating factor (G-CSF)-based mobilization schemes, an important minority
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Ozone Improves Oxygenation and Offers Organ Protection after Autologous Blood Transfusion in a Simulated Carbon Dioxide Pneumoperitoneal Environment in a Rabbit Hemorrhagic Shock Model. Transfus. Med. Hemother. (IF 1.9) Pub Date : 2023-09-27 Yu Gan,Xue Tian,Han Yao,Fei Huo,Yi Feng
Objectives Autologous blood transfusion techniques are well applied in surgery, but the red blood cells (RBCs) collected during laparoscopic surgery may forfeit their ability to oxygenate. O3 is a potent oxidation gas. This study investigates whether O3 could improve the oxygen-carrying capacity of RBCs, reduce inflammatory reactions, and offer organ protection. Methods We established a hemorrhagic
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Cytokine, Anti-SARS-CoV-2 Antibody, and Neutralizing Antibody Levels in Conventional Blood Donors Who Have Recovered from COVID-19. Transfus. Med. Hemother. (IF 1.9) Pub Date : 2023-09-22 Elvira Maličev,Klemen Žiberna,Katerina Jazbec,Ana Kolenc,Polonca Mali,Urška Rahne Potokar,Primož Rožman
Background At the beginning of the pandemic, COVID-19 convalescent plasma (CCP) containing anti-SARS-CoV-2 antibodies was suggested as a source of therapy. In the last 3 years, many trials have demonstrated the limited usefulness of CCP therapy. This led us to the hypothesis that CCP could contain other elements, along with the desired neutralizing antibodies, which could potentially prevent it from
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Mobilization and Hematopoietic Stem Cell Collection in Poor Mobilizing Patients with Lymphoma: Final Results of the German OPTIMOB Study. Transfus. Med. Hemother. (IF 1.9) Pub Date : 2023-09-21 Katharina Kriegsmann,Max Bittrich,Sandra Sauer,Carola Tietze-Stolley,Kamran Movassaghi,Matthias Grube,Vladan Vucinic,Daniela Wehler,Andreas Burchert,Martin Schmidt-Hieber,Andreas Rank,Heinz A Dürk,Bernd Metzner,Christoph Kimmich,Marcus Hentrich,Christian Kunz,Frank Hartmann,Cyrus Khandanpour,Maike de Wit,Udo Holtick,Michael Kiehl,Andrea Stoltefuß,Alexander Kiani,Ralph Naumann,Christian W Scholz,Hans-Joachim
Introduction Successful mobilization and collection of peripheral hematopoietic stem cells (HSCs) are necessary for lymphoma patients eligible for myeloablative chemotherapy with subsequent autologous stem cell transplantation (ASCT). Albeit G-CSF alone or combined with chemotherapy is well-established methods for HSC mobilization, up to 40% of the patients fail to mobilize (poor mobilizer, PM). Plerixafor
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Flow Cytometric Characterization of Hematopoietic Stem and Progenitor Cell Subpopulations in Autologous Peripheral Blood Stem Cell Preparations after Cryopreservation. Transfus. Med. Hemother. (IF 1.9) Pub Date : 2023-09-15 Anabel Heuer,Svea Löwhagen,Stefanie Uhlig,Svetlana Hetjens,Sylvia Büttner,Britta Pflästerer,Anke Diehlmann,Stefan Klein,Harald Klüter,Karen Bieback,Patrick Wuchter
Introduction Autologous stem cell transplantation is a successful routine procedure with only a small number of non-engraftment cases, although the time to hematopoietic recovery may vary considerably across patients. While CD34 has been the decisive marker for enumerating hematopoietic stem and progenitor cells (HSPCs) for more than 30 years, the impact of CD34-positive cellular subpopulations in
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Two Cases of Anti-D Alloimmunization in D-Negative Thai Patients as a Result of the Asian-Type DEL on Transfused Red Cells. Transfus. Med. Hemother. (IF 1.9) Pub Date : 2023-09-14 Kanyapon Suksard,Komon Luangtrakool,Thongbai Rungroung,Sutthisak Chamsai,Pradermchai Saetam,Kulvara Kittisares,Parichart Permpikul,Janejira Kittivorapart
Introduction DEL is known to be one of the weakest D variants, which can be detected by the adsorption-elution technique or by molecular study. Currently, in Thailand, we do not routinely test for DEL variants serologically or genetically among serologic RhD-negative blood donors. Case Presentation We reported 2 cases of alloimmunization after transfused with Rh DEL, RHD*DEL1 allele, in the Thai population
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Increased Responsiveness of Stored Platelets after Short-Term Refrigeration. Transfus. Med. Hemother. (IF 1.9) Pub Date : 2023-09-14 Julia Zeller-Hahn,Anna Kobsar,Marius Bittl,Angela Koessler,Katja Weber,Markus Boeck,Juergen Koessler
Introduction Refrigeration of platelets is considered to provide advantages in therapy of acute hemorrhage due to increased platelet responsiveness. The alleviation of inhibitory signaling caused by cold temperature (CT) has been identified as an important mechanism contributing to enhanced platelet reactivity, detectable in freshly prepared platelets within 1 h of cold storage. The aim of this study
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Moleculargenetic and in Addition Partly Discrepant Infection Serological Malaria Testing in Two Blood Donors. Transfus. Med. Hemother. (IF 1.9) Pub Date : 2023-09-11 Lutz Pichl,Katja Konietzko,Ludwig Hartmann,Bogdan Puscasu,Carlos Jiménez Klingberg
Introduction According to the guidelines (GL) valid in Germany, persons born or raised in a malaria-endemic area or had continuously stayed in a malaria-endemic area for more than 6 months may only be admitted donating blood if, among other things, validated and quality-assured laboratory diagnostics show that there is no evidence of infectivity. In a statement of the Working Group "Blood" of the Federal
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Stem Cell Mobilization, Collection, and Processing. Transfus. Med. Hemother. (IF 1.9) Pub Date : 2023-09-05 Katharina Kriegsmann,Patrick Wuchter
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Efficient Chimeric Antigen Receptor T-Cell Generation Starting with Leukoreduction System Chambers of Thrombocyte Apheresis Sets. Transfus. Med. Hemother. (IF 1.9) Pub Date : 2023-08-30 Stefani Xhaxho,Linping Chen-Wichmann,Sophie Kreissig,Roland Windisch,Adrian Gottschlich,Sayantan Nandi,Sophie Schabernack,Irmgard Kohler,Christian Kellner,Sebastian Kobold,Andreas Humpe,Christian Wichmann
Introduction Primary human blood cells represent an essential model system to study physiology and disease. However, human blood is a limited resource. During healthy donor plateletpheresis, the leukoreduction system chamber (LRSC) reduces the leukocyte amount within the subsequent platelet concentrate through saturated, fluidized, particle bed filtration technology. Normally, the LRSC is discarded
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Validation of a Parvovirus B19 NAT Assay for Screening of Umbilical Cord Blood for Allogenic Hematopoietic Stem Cell Donation. Transfus. Med. Hemother. (IF 1.9) Pub Date : 2023-08-30 Philipp Steininger,Klaus Korn,Holger Hackstein,Erwin F Strasser
Introduction Parvovirus B19 transmitted by umbilical cord blood (UCB) products may cause severe disease in allogenic hematopoietic stem cell transplant recipients. Thus, commercially available nucleic acid test (NAT) assays for highly sensitive detection of parvovirus B19 DNA validated for the specimen cord blood plasma (CBP) are required to avoid parvovirus B19 transmission by umbilical hematopoietic
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Mobilization Strategies in Myeloma Patients Intended for Autologous Hematopoietic Cell Transplantation. Transfus. Med. Hemother. (IF 1.9) Pub Date : 2023-08-24 Esa Jantunen,Anu Partanen,Antti Turunen,Ville Varmavuo,Raija Silvennoinen
Background Multiple myeloma is currently the leading indication for autologous hematopoietic cell transplantation (AHCT). A prerequisite for AHCT is mobilization and collection of adequate blood graft to support high-dose therapy. Current mobilization strategies include granulocyte colony-stimulating factor (G-CSF) alone or in combination with chemotherapy most commonly cyclophosphamide (CY). More
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CD34+ Cell Mobilization, Autograft Cellular Composition and Outcome in Mantle Cell Lymphoma Patients. Transfus. Med. Hemother. (IF 1.9) Pub Date : 2023-08-23 Antti Samuli Turunen,Outi Kuittinen,Hanne Kuitunen,Kaija Vasala,Karri Penttilä,Minna Harmanen,Leena Keskinen,Pentti Mäntymaa,Jukka Pelkonen,Ville Varmavuo,Esa Jantunen,Anu Partanen
Backgound Autologous stem cell transplantation (ASCT) is a standard treatment in transplant-eligible mantle cell lymphoma (MCL) patients after first-line chemoimmunotherapy. Study Design and Methods This prospective multicenter study evaluated the impact of CD34+ cell mobilization and graft cellular composition analyzed by flow cytometry on hematologic recovery and outcome in 42 MCL patients. Results
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Quartic CAR-T Cell Bridging to Twice Allo-HSCT Therapy in a Patient with Acute Lymphoblastic Leukemia. Transfus. Med. Hemother. (IF 1.9) Pub Date : 2023-08-22 Qing Zhang,Yi Dong,Zhimin Zhai,Qianshan Tao
Introduction Chimeric antigen receptor T (CAR-T) cell therapy is an effective bridging treatment for allogeneic hematopoietic stem cell transplantation (allo-HSCT) in relapsed or refractory acute lymphoblastic leukemia (ALL). However, repetitive CAR-T cell therapy and allo-HSCT can only be performed in a few patients because of technical difficulties and patients' physical, economic, and social conditions
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Implementation of a Bleeding Management Algorithm in Liver Transplantation: A Pilot Study. Transfus. Med. Hemother. (IF 1.9) Pub Date : 2023-08-03 Ignacio A Sarmiento,María F Guzmán,Javier Chapochnick,Jens Meier
Objectives The aims of the study were to compare the consumption of blood products before and after the implementation of a bleeding management algorithm in patients undergoing liver transplantation and to determine the feasibility of a multicentre, randomized study. Background Liver transplantation remains the only curative therapy for patients with end-stage liver disease, but it carries a high risk
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The Putative Role of the Transient Receptor Potential Ion Channel of Vanilloid Type 2 in Red Blood Cell Storage Lesions. Transfus. Med. Hemother. (IF 1.9) Pub Date : 2023-07-17 Nicoletta Murciano,Lars Kaestner
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Allogeneic Blood Transfusion and Risk of Postoperative Complications in Patients with Mild and Moderate Anemia of Any Cause? A Retrospective Cohort Study in Total Revision Hip Surgery. Transfus. Med. Hemother. (IF 1.9) Pub Date : 2023-07-12 Henning Uden,Franziska Büttner,Christian von Heymann,Michael Krämer,Lutz Kaufner,Gerald Vorderwülbecke,Sebastian Hardt,Jochen Kruppa,Felix Balzer,Claudia Spies
Introduction Patients undergoing revision total hip surgery (RTHS) have a high prevalence of mild and moderate preoperative anemia, associated with adverse outcomes. The aim of this study was to investigate the association of perioperative allogeneic blood transfusions (ABT) and postoperative complications in preoperatively mild compared to moderate anemic patients undergoing RTHS who did not receive
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Impact of Additional Administration of von Willebrand Factor Concentrates to Thrombocyte Transfusion in Perioperative Bleeding in Cardiac Surgery. Transfus. Med. Hemother. (IF 1.9) Pub Date : 2023-07-11 Katrin Ledergerber,Alexa Hollinger,Sibylle Zimmermann,Atanas Todorov,Maren Trutmann,Laura Gallachi,Lena Anna Gschwandtner,Lisa Andrea Ryser,Caroline Eva Gebhard,Daniel Bolliger,Andreas Buser,Dimitrios Athanasios Tsakiris,Martin Siegemund
Background Von Willebrand factor (vWF) is an important part of blood coagulation since it binds platelets to each other and to endothelial cells. In traumatic and surgical haemorrhage, both blood cells and plasmatic factors are consumed, leading to consumption coagulopathy and fluid resuscitation. This often results in large amounts of crystalloids and blood products being infused. Additional administration
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Predicting Successful Hematopoietic Stem Cell Collection in Healthy Allogeneic Donors. Transfus. Med. Hemother. (IF 1.9) Pub Date : 2023-06-30 Sabine Kayser,Richard F Schlenk,Marcus Steiner,Harald Klüter,Patrick Wuchter
Introduction Collection of peripheral blood stem cells (PBSCs) from healthy donors is a well-established process. We aimed to identify factors predictive of successful CD34+ PBSC collection and established a formula capable of predicting CD34+ cell yield. Methods We retrospectively evaluated 588 healthy adult donors (median age 29 years, range 18-69 years) at our institution from 2017 to 2022. The
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High-Throughput CD36 Phenotyping on Human Platelets Based on Sandwich ELISA and Mutant Gene Analysis. Transfus. Med. Hemother. (IF 1.9) Pub Date : 2023-06-27 Honghong He,Longhai Tang,Yiming Jin,Yujue Wang,Hongmei Wang,Shaohua Ding,Yezhou Chen,Jingjing Tian,Mingyuan Wang,Shengbao Duan
Background CD36 deficiency is closely associated with fetal/neonatal alloimmune thrombocytopenia, platelet transfusion refractoriness, and other hemorrhage disorders, particularly in Asian and African populations. There is a clinical need for rapid and high-throughput methods of platelet CD36 (pCD36) phenotyping to improve the availability of CD36 typing of donors and assist clinical blood transfusions
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The Ratio of Intraoperative Red Blood Cell Transfusion to Blood Loss Associated with Early Postoperative Complications in Pediatric Liver Transplantation Patients. Transfus. Med. Hemother. (IF 1.9) Pub Date : 2023-06-23 Yiming Ma,Cheng Li,Liying Sun,Xiaofei Li
Introduction Liver transplantation (LT) is an operation purposed to save the lives of children with acute or chronic liver diseases, hepatic tumors, and some genetic and metabolic diseases. However, patients who underwent LT have a significant risk of intraoperative blood loss and red blood cell (RBC) transfusion, especially in pediatric patients. Methods In this study, 569 pediatric patients (<18
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The Impact of Pathogen Reduction on Total IgG and IgG Subclass Profiles of Convalescent Plasma. Transfus. Med. Hemother. (IF 1.9) Pub Date : 2023-06-21 Tomasz Wasiluk,Magdalena Sredzinska,Anna Rogowska,Magdalena Dzieniszewska,Agnieszka Zebrowska,Barbara Boczkowska-Radziwon,Katarzyna Gagola,Anna Stasiak-Barmuta,Marcus Picard-Maureau,Piotr Radziwon
Introduction In case of newly emerging pathogens, convalescent plasma (CP) is often the only early available treatment option. It has been shown that different IgG subclasses contribute differently to CP neutralizing activity. As CP donors often have a risk profile like first-time donors, especially with respect to window-period viral transmission, pathogen reduction (PR) could mitigate that risk.
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HLA Linked Regional Differences in Donor Selection: A 9-Year Study of the Swiss Registry. Transfus. Med. Hemother. (IF 1.9) Pub Date : 2023-06-16 Oliver Kürsteiner,Grazia Nicoloso,Tizian Demont,Marcel Büchler,Jean-Marie Tiercy
Introduction The large HLA diversity in worldwide populations is a major challenge for matched unrelated haematopoietic stem cell (HSC) donor searches. The impact of regional diversity on the effective HSC donor selection has not been documented so far for national registries. Methods The aim of the study was to analyse the 532 consecutive work-up (WU) requests received by Swiss Blood Stem Cells (SBSC)
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Peripartum Haemorrhage: Haemostatic Aspects of the Updated Peripartum Haemorrhage Guideline of the German-Speaking Countries. Transfus. Med. Hemother. (IF 1.9) Pub Date : 2023-06-06 Heiko Lier,Thorsten Annecke,Thierry Girard,Georg Pfanner,Wolfgang Korte,Oliver Tiebel,Dietmar Schlembach,Christian von Heymann
Background Peripartum haemorrhage (PPH) is a potentially life-threatening complication. Although still rare, the incidence of peripartal haemorrhage is rising in industrialised countries and refractory bleeding remains among the leading causes of death in the peripartal period. Summary The interdisciplinary German, Austrian, and Swiss guideline on "Peripartum Haemorrhage: Diagnostics and Therapies"
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The Impact of Digital Transformation on Blood Donation and Donor Characteristics. Transfus. Med. Hemother. (IF 1.9) Pub Date : 2023-06-05 Norbert Niklas,Claudia Loimayr,Julia Lenz,Susanne Süßner,Gerhard Schuster,David Jungwirth,Werner Watzinger,Stephan Federsel
Introduction The management of an adequate donor pool is a constant and challenging task for blood centers in order to provide blood supply. New methods are required to streamline processes and attract (new) donors on a sustained basis. We present a digitalization method without media disruption and show the impact on our donors and their behavior. Methods We designed and created a blood donation app
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Impact of Clinical Parameters and Induction Regimens on Peripheral Blood Stem-Cell Mobilization and Collection in Multiple Myeloma Patients. Transfus. Med. Hemother. (IF 1.9) Pub Date : 2023-06-05 Sandra Sauer,Lennart Hieke,Juliane Brandt,Carsten Müller-Tidow,Anita Schmitt,Joseph Kauer,Katharina Kriegsmann
Introduction High-dose chemotherapy (HDCT) followed by autologous blood stem-cell transplantation (ABSCT) remains the standard consolidation therapy for newly diagnosed eligible multiple myeloma (MM) patients. As a prerequisite, peripheral blood stem cells (PBSCs) must be mobilized and collected by leukapheresis (LP). Many factors can hamper PBSC mobilization/collection. Here, we provide a comprehensive
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Big Data in Transfusion Medicine and Artificial Intelligence Analysis for Red Blood Cell Quality Control. Transfus. Med. Hemother. (IF 1.9) Pub Date : 2023-05-25 Marcelle G M Lopes,Steffen M Recktenwald,Greta Simionato,Hermann Eichler,Christian Wagner,Stephan Quint,Lars Kaestner
Background "Artificial intelligence" and "big data" increasingly take the step from just being interesting concepts to being relevant or even part of our lives. This general statement holds also true for transfusion medicine. Besides all advancements in transfusion medicine, there is not yet an established red blood cell quality measure, which is generally applied. Summary We highlight the usefulness
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Omics and Machine Learning in Transfusion Medicine. Transfus. Med. Hemother. (IF 1.9) Pub Date : 2023-05-25 Angelo D'Alessandro,Peter Bugert
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Omics Technologies in Veterinary Medicine: Literature Review and Perspectives in Transfusion Medicine. Transfus. Med. Hemother. (IF 1.9) Pub Date : 2023-05-25 Arianna Miglio,Valentina Cremonini,Leonardo Leonardi,Elisabetta Manuali,Paola Coliolo,Olimpia Barbato,Cecilia Dall'Aglio,Maria Teresa Antognoni
Background Omics technologies represent a new analytical approach that allows a full cellular readout through the simultaneous analysis of thousands of molecules. The application of such technologies represents a flourishing field of research in human medicine, especially in transfusion medicine, while their application in veterinary medicine still needs to be developed. Summary Omics technologies
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Different Expression Profiles of Exosomal circRNAs from Apheresis Platelets during Storage. Transfus. Med. Hemother. (IF 1.9) Pub Date : 2023-05-09 Jingfu Liu,Shan Chen,Zhen Li,Rong Lu,Xianren Ye
Introduction Bioactive substances of stored platelets change during the stored periods. Exosomes are reported to be increased during platelet storage. Circular RNAs (circRNAs) are one of the main components in exosomes. It is the purpose of this study to investigate the different expression of exosomal circRNAs during storage. Methods Apheresis platelets were collected from 7 healthy volunteers and
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Rapid Identification of Seven Common ABO Alleles by Two-Dimensional Polymerase Chain Reaction Technology Transfus. Med. Hemother. (IF 1.9) Pub Date : 2023-04-24 Jin Chen, Yuxia Zhan, Jun Zhang, Yang Yu, Shuang Yao, Guanghua Luo
Introduction: The molecular biology detection technology of the human ABO blood group system makes up for the limitations in many aspects compared with conventional serological typing technology. This study aimed to establish a new method to identify seven common ABO alleles (ABO*A1.01, ABO*A1.02, ABO*A2.01, ABO*B.01, ABO*O.01.01, ABO*O.01.02, and ABO*O.02.01) by two-dimensional polymerase chain reaction
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Effect of Plasma Unit Weight and Donor Sex on Post-Donation Citrate Level: An Experimental Study on Plasmapheresis Donors Transfus. Med. Hemother. (IF 1.9) Pub Date : 2023-04-20 Chiara Marraccini, Davide Schiroli, Pamela Mancuso, Giuseppe Molinari, Agnese Razzoli, Gaia Gavioli, Tommaso Fasano, Roberto Baricchi, Paolo Giorgi Rossi, Lucia Merolle
Introduction: Plasmapheresis donation is considered safe and well tolerated, although long-term effects need to be clarified. The volumes of anticoagulant (ACD-A) used are variable and depend primarily on hematocrit (HCT), total blood processed, amount of plasma collected, and donor characteristics. To elucidate the effect of the plasma unit weight setting on plasmapheresis efficiency and ACD-A distribution
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Defining Current Patterns of Blood Product Use during Intensive Induction Chemotherapy in Newly Diagnosed Acute Myeloid Leukemia Patients Transfus. Med. Hemother. (IF 1.9) Pub Date : 2023-04-13 Liron Miller, Mor Freed-Freundlich, Avichai Shimoni, Tamer Hellou, Abraham Avigdor, Mudi Misgav, Jonathan Canaani
Introduction: Blood product transfusion retains a critical role in the supportive care of patients with acute myeloid leukemia (AML). Whereas previous studies have shown increased transfusion dependency to portend inferior outcome, predictive factors of an increased transfusion burden and the prognostic impact of transfusion support have not been assessed recently. Methods/Patients: We performed a
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Too Early to Abandon Convalescent Plasma for Supportive Treatment of COVID-19 Transfus. Med. Hemother. (IF 1.9) Pub Date : 2023-04-03 Rainer Seitz, Lutz Gürtler, Ute Vahlensieck, Anneliese Hilger, Wolfgang Schramm
The authors of a systematic review and meta-analysis conclude that the benefit of convalescent plasma (CP) in the treatment of COVID-19 is limited. Among other systematic reviews, only one found an indication of benefit of CP. However, a meta-analysis needs a focused and meaningful clinical question and should include studies which are designed to test a reasonable hypothesis. Clinical trials to support
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Association of Circulating Procoagulant Microvesicles with Painful Vaso-Occlusive Crisis in Sickle Cell Disease Transfus. Med. Hemother. (IF 1.9) Pub Date : 2023-03-30 Akbar Hashemi Tayer, Reza Ranjbaran, Maryam Kamravan, Mojdeh Abbasi, Reyhaneh Zareian
Introduction: Thrombotic complication is one of the features of sickle cell disease (SCD), characterized by appearance of phosphatidylserine on the outer membrane of sickle-shaped red blood cells and most abundantly on membrane protrusions called microvesicles (MVs). However, the exact mechanism by which MVs may enhance coagulant activity in SCD patients has not been fully addressed. The aim of this
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Autologous Stem Cell Collection after Daratumumab, Bortezomib, Thalidomide, and Dexamethasone versus Bortezomib, Cyclophosphamide, and Dexamethasone in Newly Diagnosed Multiple Myeloma Transfus. Med. Hemother. (IF 1.9) Pub Date : 2023-03-24
Introduction: In transplant-eligible, newly diagnosed multiple myeloma (NDMM) patients, autologous peripheral blood stem cell (PBSC) collection is usually pursued after induction therapy. While induction regimens are constantly refined regarding response, their impact on PBSC collection is not fully studied. The inclusion of the anti-CD38 antibody daratumumab into induction therapy significantly improved
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Principles of Therapeutic Apheresis in Neurological Disease Transfus. Med. Hemother. (IF 1.9) Pub Date : 2023-03-14
Background: Therapeutic plasma exchange (TPE) is a well-known apheresis technology since many years and is available worldwide. Myasthenia gravis is one of the first neurological diseases successfully treated with TPE. TPE is also frequently applied in acute inflammatory demyelinating polyradiculoneuropathy (Guillain-Barré syndrome). Both neurological disorders are immunologically mediated and might
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Circulating Iron in Patients with Sickle Cell Disease Mediates the Release of Neutrophil Extracellular Traps Transfus. Med. Hemother. (IF 1.9) Pub Date : 2023-03-15 Kristof Van Avondt, Marein Schimmel, Ingrid Bulder, Gerard van Mierlo, Erfan Nur, Robin van Bruggen, Bart J. Biemond, Brenda M. Luken, Sacha Zeerleder
Introduction: Neutrophils promote chronic inflammation and release neutrophil extracellular traps (NETs) that can drive inflammatory responses. Inflammation influences progression of sickle cell disease (SCD), and a role for NETs has been suggested in the onset of vaso-occlusive crisis (VOC). We aimed to identify factors in the circulation of these patients that provoke NET release, with a focus on
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Predicting Individual Patient Platelet Demand in a Large Tertiary Care Hospital Using Machine Learning Transfus. Med. Hemother. (IF 1.9) Pub Date : 2023-03-09
Introduction: An increasing shortage of donor blood is expected, considering the demographic change in Germany. Due to the short shelf life and varying daily fluctuations in consumption, the storage of platelet concentrates (PCs) becomes challenging. This emphasizes the need for reliable prediction of needed PCs for the blood bank inventories. Therefore, the objective of this study was to evaluate