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Exosomes derived from hypoxic alveolar epithelial cells promote the phenotypic transformation of pulmonary artery smooth muscle cells via the Rap1 pathway Exp. Lung Res. (IF 1.5) Pub Date : 2024-09-17 Guifang Sun, Fangyun Zhao, Yusen Feng, Fei Liu, Xingrui Liu, Yue Jiang, Yating Gao, Jian Hu, Feifei Zhou, Yongju Yang, Zhiqin Du, Caiyan Zhu, Bin Liu
Background: Hypoxic pulmonary hypertension (HPH) is one of the important pathophysiological changes in chronic pulmonary heart disease. Hypoxia promotes the phenotypic transformation of pulmonary a...
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Sulfotransferase homolog 2 receptors blockade on monocyte subsets along with their inflammatory cytokines for septic lung injury Exp. Lung Res. (IF 1.5) Pub Date : 2024-09-06 Peng Wang, Shuqi Yang, Changcheng Li, Baohua Ma, Mengqiu Yi, Xiaobo Chen, Min Yu
To observe the dynamic changes in monocyte subsets during septic lung injury and to assess the anti-inflammatory role of the sulfotransferase homolog 2 (ST2) receptor.Dynamic changes of monocyte su...
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Leucine rich α2 glycoprotein 1 derived from malignant pleural mesothelioma cells facilitates macrophage M2 phenotypes Exp. Lung Res. (IF 1.5) Pub Date : 2024-07-21 Dandan Wang, Wenjing Pei, Yanfei Liu, Rongliang Mo, Xinru Li, Wenhui Gu, Yi Su, Jing Ye, Jiegou Xu, Dahai Zhao
Background: Macrophages constitute the main part of infiltrating immune cells in Malignant pleural mesothelioma (MPM) and abnormally high ratios of M2 macrophages are present in both pleural effusi...
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High oxygen exposure’s impact on newborn mice: Temporal changes observed via micro-computed tomography Exp. Lung Res. (IF 1.5) Pub Date : 2024-07-08 Himeko Sato, Akie Kato, Hiroyuki Adachi, Kiichi Takahashi, Hirokazu Arai, Masato Ito, Fumihiko Namba, Tsutomu Takahashi
Bronchopulmonary dysplasia (BPD) impacts life expectancy and long-term quality of life. Currently, BPD mouse models exposed to high oxygen are frequently used, but to reevaluate their relevance to ...
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Treatment with inhaled aerosolised ethanol reduces viral load and potentiates macrophage responses in an established influenza mouse model Exp. Lung Res. (IF 1.5) Pub Date : 2024-04-29 David G. Hancock, Luke Berry, Naomi M. Scott, Kyle T. Mincham, William Ditcham, Alexander N. Larcombe, Barry Clements
Treatment options for viral lung infections are currently limited. We aimed to explore the safety and efficacy of inhaled ethanol in an influenza-infection mouse model.In a safety and tolerability ...
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Inhibition of GBP5 activates autophagy to alleviate inflammatory response in LPS-induced lung injury in mice Exp. Lung Res. (IF 1.5) Pub Date : 2024-04-20 Jialin Li, Kexuan Liu, Wenjuan He, Wencai Zhang, Yongchao Li
Pulmonary emphysema is a condition that causes damage to the lung tissue over time. GBP5, as part of the guanylate-binding protein family, is dysregulated in mouse pulmonary emphysema. However, the...
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Differential changes in expression of inflammatory mRNA and protein after oleic acid-induced acute lung injury Exp. Lung Res. (IF 1.5) Pub Date : 2024-04-16 Regina Golding, Rudolf K. Braun, Lorenzo Miller, Michael Lasarev, Timothy A. Hacker, Allison C. Rodgers, Ava Staehler, Marlowe W. Eldridge, Awni Al-Subu
Background: Acute Respiratory Distress syndrome (ARDS) is a clinical syndrome of noncardiac pulmonary edema and inflammation leading to acute respiratory failure. We used the oleic acid infusion pi...
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CLCA1 exacerbates lung inflammation via p38 MAPK pathway in acute respiratory distress syndrome Exp. Lung Res. (IF 1.5) Pub Date : 2024-04-10 Xing Lv, Long Zheng, Tianxiang Zhang, Weijia Wang, Yuanyuan Chen, Jing Li, Zhigui Cai, Xingxing Guo, Liqiang Song
Recent research has revealed that airway epithelial calcium-activated chloride channel-1 (CLCA1) is implicated in the inflammation of multiple human respiratory diseases, but the specific role in a...
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Exploring the role of oral microbiome dysbiosis in cardiometabolic syndrome and smoking Exp. Lung Res. (IF 1.5) Pub Date : 2024-03-27 Layla I. Mohammed, Zain Zaki Zakaria, Fatiha M. Benslimane, Maha Al-Asmakh
Oral microbiome research has gained significant interest in recent years due to its potential impact on overall health. Smoking has been identified as a significant modulator of the oral microbiome...
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Protective effect of liver X receptor on cigarette smoke and lipopolysaccharide induced airway inflammation and emphysema in mice Exp. Lung Res. (IF 1.5) Pub Date : 2024-03-21 Fenfang Yu, Lin Gao, Ke Xu, Xiaobing Yang, Junran Zhang, Yu Tang, Zhifei Ma, Wei Gu, Bining Wu, Ying Shi
The aim of this study is to assess the impact of Liver X receptors (LXRs) on airway inflammation, airway remodeling, and lipid deposition induced by cigarette smoke and lipopolysaccharide (LPS) exp...
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Exosomal miR-223 promotes ARDS by targeting insulin-like growth factor 1 receptor: A cell communication study Exp. Lung Res. (IF 1.5) Pub Date : 2024-03-01 Miaomiao Li, Lilei Zhuang, Tao Jiang, Li Sun
Acute respiratory distress syndrome (ARDS) is a respiratory failure syndrome characterized by hypoxemia and changes in the respiratory system. ARDS is the most common cause of death in COVID-19 dea...
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BCL6 attenuates hyperoxia-induced lung injury by inhibiting NLRP3-mediated inflammation in fetal mouse Exp. Lung Res. (IF 1.5) Pub Date : 2024-02-29 Dan Chen, Hui-min Zhao, Xian-hui Deng, Sheng-peng Li, Mei-hui Zhou, Ya-xian Wu, Ying Tong, Ren-qiang Yu, Qing-feng Pang
The transcriptional repressor B-cell lymphoma 6 (BCL6) has been reported to inhibit inflammation. So far, experimental evidence for the role of BCL6 in bronchopulmonary dysplasia (BPD) is lacking. ...
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Metformin alleviates lung ischemia–reperfusion injury in a rat lung transplantation model Exp. Lung Res. (IF 1.5) Pub Date : 2024-02-05 Huizhi Yu, Jing Wang, Mingzhao Liu, Chunlan Hu, Jiaojiao Sun, Bo Xu, Shunmei Lu, Dongxiao Huang, Qingfeng Pang, Chunxiao Hu
Background: Lung ischemia–reperfusion injury (LIRI) is among the complications observed after lung transplantation and is associated with morbidity and mortality. Preconditioning of the donor lung ...
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Hsa_circ_0008833 promotes COPD progression via inducing pyroptosis in bronchial epithelial cells Exp. Lung Res. (IF 1.5) Pub Date : 2024-01-17 Tian Xie, Zehua Yang, Shaojing Xian, Qi Lin, Linhui Huang, Yipeng Ding
Purpose: Chronic obstructive pulmonary disease (COPD) is a common respiratory disorder. Pyroptosis represents a distinctive form of inflammatory cell death that is mediated through the activation o...
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Stevioside attenuates bleomycin-induced pulmonary fibrosis by activating the Nrf2 pathway and inhibiting the NF-κB and TGF-β1/Smad2/3 pathways Exp. Lung Res. (IF 1.5) Pub Date : 2023-12-03 Wei Hao, Ting-ting Yu, Dong-ze Zuo, Heng-zhao Hu, Ping-ping Zhou
Objective: This study aimed to investigate the effects of stevioside (STE) on pulmonary fibrosis (PF) and the potential mechanisms. Methods: In this study, a mouse model of PF was established by a ...
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METTL14 knockdown inhibits the pyroptosis in the sepsis-induced acute lung injury through regulating the m6A modification of NLRP3 Exp. Lung Res. (IF 1.5) Pub Date : 2023-12-04 Jianting Gao, Huizhen Chen, Yong Zhang, Sun Yu, Yiyi Wu, Qiuyan Wang, Zhenfei Yu
Background: Sepsis has become one of the main factors inducing the development of acute lung injury (ALI) in clinical practice. Currently, inhibiting the activation of NLRP3 mediated pyroptosis is ...
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Impact of lipopolysaccharide-induced acute lung injury in aged mice Exp. Lung Res. (IF 1.5) Pub Date : 2023-11-25 Sukjin Bae, In Kyoung Kim, Jeonghyeon Im, Heayon Lee, Sang Haak Lee, Sei Won Kim
Study Aim: As the geriatric population rapidly expands, there has been a concurrent increase in elderly admissions to intensive care units (ICUs). Acute lung injury (ALI) is a prevalent reason for ...
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Rapamycin attenuates pyroptosis by suppressing mTOR phosphorylation and promoting autophagy in LPS-induced bronchopulmonary dysplasia Exp. Lung Res. (IF 1.5) Pub Date : 2023-10-24 Feng Zhang, Minrong Wang, Zhongni Li, Jiehong Deng, Yang Fan, Zhixian Gou, Yue Zhou, Li Huang, Liqun Lu
Bronchopulmonary dysplasia (BPD) is associated with poor survival in preterm infants. Intrauterine infection can aggravate the degree of obstruction of alveolar development in premature infants; ho...
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Effect of mechanical ventilation under intubation on respiratory tract change of bacterial count and alteration of bacterial flora Exp. Lung Res. (IF 1.5) Pub Date : 2023-10-03 Chen Xue-Meng, Liu Gao-Wang, Ling Xiao-Mei, Zeng Fan-Fang, Xiao Jin-Fang
Background: The most common ‘second strike’ in mechanically ventilated patients is a pulmonary infection caused by the ease with which bacteria can invade and colonize the lungs due to mechanical v...
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Alveolar epithelial cell growth hormone releasing hormone receptor in alveolar epithelial inflammation Exp. Lung Res. (IF 1.5) Pub Date : 2023-08-16 Tengjiao Cui, Medhi Wangpaichitr, Andrew V. Schally, Anthony J. Griswold, Irving Vidaurre, Wei Sha, Robert M. Jackson
Abstract Purpose: Growth hormone-releasing hormone (GHRH) is a 44-amino acid peptide that regulates growth hormone (GH) secretion. We hypothesized that GHRH receptor (GHRH-R) in alveolar type 2 (AT2) cells could modulate pro-inflammatory and possibly subsequent pro-fibrotic effects of lipopolysaccharide (LPS) or cytokines, such that AT2 cells could participate in lung inflammation and fibrosis. Methods:
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Ultrastructural analysis of the intracellular surfactant in lungs of healthy and ovalbumin sensitized and challenged Brown Norway rats Exp. Lung Res. (IF 1.5) Pub Date : 2023-07-21 Andreas Schmiedl, Stefanie Frank, Thomas Tschernig, Jens M. Hohlfeld
Abstract Introduction: In human and experimentally induced asthma, a dysfunction of the intra-alveolar-surface active agent (surfactant) has been demonstrated. Type II alveolar epithelial cells (AEII) synthesize, secrete and recycle surfactant. Prior to secretion, intracellular surfactant is stored in specific secretory organelles of AEII. The lamellar bodies (Lb) represent its ultrastructural correlate
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Force adaptation through the intravenous route in naïve mice Exp. Lung Res. (IF 1.5) Pub Date : 2023-07-21 Magali Boucher, Cyndi Henry, Ynuk Bossé
Abstract Aim of the study: Force adaptation is a process whereby the contractile capacity of the airway smooth muscle increases during a sustained contraction (aka tone). Tone also increases the response to a nebulized challenge with methacholine in vivo, presumably through force adaptation. Yet, due to its patchy pattern of deposition, nebulized methacholine often spurs small airway narrowing heterogeneity
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Chronic exposure to ampicillin alters lung microbial composition in laboratory rat Exp. Lung Res. (IF 1.5) Pub Date : 2023-06-15 Ping Chen, Tingting Hu, Haonan Jiang, Bing Li, Guiying Li, Pixin Ran, Yumin Zhou
ABSTRACTS Purpose High-throughput sequencing technologies have revealed that the lungs contain a variety of low biomass microbiota associated with various lung diseases. Rat model is an important tool to understand the possible causal relationship between pulmonary microbiota and diseases. Antibiotic exposure can alter the microbiota, however, a direct influence of long-term ampicillin exposure on
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Melatonin attenuates lung ischemia-reperfusion injury through SIRT3 signaling-dependent mitophagy in type 2 diabetic rats Exp. Lung Res. (IF 1.5) Pub Date : 2023-06-02 Zhiqiang Song, Congmin Yan, Yuanbo Zhan, Qiujun Wang, Yina Zhang, Tao Jiang
Abstract Background: Lung ischemia-reperfusion injury (LIRI) remains the major cause of primary lung dysfunction after lung transplantation. Diabetes mellitus (DM) is an independent risk factor for morbidity and mortality following lung transplantation. Mitochondrial dysfunction is recognized as a key mediator in the pathogenesis of diabetic LIRI. Melatonin has been reported to be a safe and potent
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Inhibition of cGAS aggravated the host inflammatory response to Aspergillus fumigatus Exp. Lung Res. (IF 1.5) Pub Date : 2023-05-16 Mei Peng, Xiujun Li, Xiaobing Zhang, Li Peng
Backgroud: Aspergillus fumigatus (A. fumigatus) is a clinically important fungal pathogen. Invasive pulmonary aspergillosis (IPA) is the main fungal infection with increased morbidity and mortality...
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The multiphasic TNF-α-induced compromise of Calu-3 airway epithelial barrier function Exp. Lung Res. (IF 1.5) Pub Date : 2023-03-31 Katherine M. DiGuilio, Elizabeth Rybakovsky, Yoongyeong Baek, Mary Carmen Valenzano, James M. Mullin
Abstract Purpose: Airway epithelial barrier leak and the involvement of proinflammatory cytokines play a key role in a variety of diseases. This study evaluates barrier compromise by the inflammatory mediator Tumor Necrosis Factor-α (TNF-α) in the human airway epithelial Calu-3 model. Methods: We examined the effects of TNF-α on barrier function in Calu-3 cell layers using Transepithelial Electrical
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HIF and ER stress are involved in TGFβ1-mediated wound closure of alveolar epithelial cells Exp. Lung Res. (IF 1.5) Pub Date : 2023-03-22 Eva Delbrel, Nicolas Voituron, Emilie Boncoeur
Abstract Purpose: Alveolar epithelium dysfunction is associated with a very large spectrum of disease and an abnormal repair capacity of the airway epithelium has been proposed to explain the pathogenesis of Idiopathic Pulmonary Fibrosis (IPF). Following epithelium insult, the damaged cells will activate pathways implicated in the repair process, including proliferation and acquisition of migratory
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House dust mite-induced endoplasmic reticulum stress mediates MUC5AC hypersecretion via TBK1 in airway epithelium Exp. Lung Res. (IF 1.5) Pub Date : 2023-01-31 Jun Deng, Hongmei Tang, Yun Zhang, Xiefang Yuan, Ning Ma, Hang Hu, Xiaoyun Wang, Chunfeng Liu, Guofeng Xu, Yuejiao Li, Songping Wang, Linlin Guo, Xing Wang
Abstract Purpose: Endoplasmic reticulum (ER) stress regulates mucus hypersecretion, and may activate downstream factors via TBK1 signaling to induce gene expression. However, it remains unclear whether ER stress promotes airway mucus secretion through the TBK1 pathway. We aimed to investigate the role of the TBK1 pathway in the regulation of MUC5AC expression in a mouse model of house dust mite (HDM)-induced
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Hypoxic conditions promote a proliferative, poorly differentiated phenotype in COPD lung tissue progenitor cells in vitro Exp. Lung Res. (IF 1.5) Pub Date : 2023-01-19 Tina P. Dale, Michael D. Santer, Mohammed Haris, Wei Zuo, Nicholas R. Forsyth
Abstract Purpose Chronic obstructive pulmonary disease (COPD) patients experience hypoxemia and lung tissue hypoxia, causing vasoconstriction, and at its most severe Cor pulmonale. However, minimal attention has been given to the effects of hypoxia at the cellular level. We hypothesize that a persistent progenitor cell population undergoes an aberrant differentiation process, influenced by changes
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Cigarette smoke extract-induced inflammatory response via inhibition of the TFEB-mediated autophagy in NR8383 cells Exp. Lung Res. (IF 1.5) Pub Date : 2023-01-13 Shu-wen Xu, Yu-jie Zhang, Wen-mei Liu, Xin-fang Zhang, Yuan Wang, Shui-ying Xiang, Jing-chao Su, Zi-bing Liu
Abstract Objective: Chronic pulmonary inflammation caused by long-term smoking is the core pathology of COPD. Alveolar macrophages (AMs) are involved in the pulmonary inflammation of COPD. The accumulation of damaged materials caused by impaired autophagy triggers inflammatory response in macrophages. As a key transcription regulator, transcription factor EB (TFEB) activates the transcription of target
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p14ARF ameliorates inflammation and airway remodeling in nitric acid aerosol inhalation-induced bronchiolitis obliterans Exp. Lung Res. (IF 1.5) Pub Date : 2023-01-09 Ting Yang, Chang Xu, Niu Ding, Shujuan Luo, Bichen Wu, Shijie Jin, Yanping Chen
Abstract Background To investigate the protective effect of p14ARF in a nitric acid (NA) aerosol inhalation-induced bronchiolitis obliterans (BO) mouse model and its potential regulatory mechanism. Methods A BO mouse model was established by NA aerosol inhalation. The expressions of p14ARF, phosphatidylinositol-3-kinase (PI3K), and protein kinase B (AKT) were detected by quantitative reverse transcription
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Comparison of hypoxia-induced pulmonary hypertension rat models caused by different hypoxia protocols Exp. Lung Res. (IF 1.5) Pub Date : 2022-11-24 Gexiang Cai, Yaxin Zhang, Xinghong Wang, Shini Li, Yushan Cheng, Xiaoying Huang
Abstract Background and aim: Pulmonary hypertension (PH) is a serious and even fatal disorder with limited treatment strategies. The hypoxia-induced pulmonary hypertension (HPH) rat model is commonly used in this field. While the HPH rat model has strong predictability and repeatability, the model is a chronic model, making it time-consuming, costly, and complicated and limiting the progress of the
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Changes in lung sialidases in male and female mice after bleomycin aspiration Exp. Lung Res. (IF 1.5) Pub Date : 2022-11-16 Darrell Pilling, Kyle Sahlberg, Wensheng Chen, Richard H. Gomer
ABSTRACT Aim of the study: Sialidases, also called neuraminidases, are enzymes that cleave terminal sialic acids from glycoconjugates. In humans and mice, lung fibrosis is associated with desialylation of glycoconjugates and upregulation of sialidases. There are four mammalian sialidases, and it is unclear when the four mammalian sialidases are elevated over the course of inflammatory and fibrotic
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Proteomic global proteins analysis in blast lung injury reveals the altered characteristics of crucial proteins in response to oxidative stress, oxidation-reduction process and lipid metabolic process Exp. Lung Res. (IF 1.5) Pub Date : 2022-11-08 Peifang Cong, Changci Tong, Shun Mao, Xiuyun Shi, Ying Liu, Lin Shi, Hongxu Jin, Yunen Liu, Mingxiao Hou
ABSTRACT Background: Blast lung injury (BLI) is the most common fatal blast injury induced by overpressure wave in the events of terrorist attack, gas and underground explosion. Our previous work revealed the characteristics of inflammationrelated key proteins involved in BLI, including those regulating inflammatory response, leukocyte transendothelial migration, phagocytosis, and immune process. However
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Prevention of submicron aerosolized particle dispersion: evaluation of an aerosol box using a pediatric simulation model Exp. Lung Res. (IF 1.5) Pub Date : 2022-10-21 Laurence Tabone, Dominic Rivest, Arielle Levy, Michael Buyck, Philippe Jouvet, Carl-Eric Aubin, Tine François, Etienne Robert, Florent Baudin
ABSTRACT Background and Aim: The SplashGuard CG (SG) is a barrier enclosure developed to protect healthcare workers from SARS-CoV-2 transmission during aerosol-generating procedures. Our objective was to evaluate the protection provided by the SG against aerosolized particles (AP), using a pediatric simulation model of spontaneous ventilation (SV) and noninvasive ventilation (NIV). Methods: An aerosol
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Effect of mycobacterial proteins that target mitochondria on the alveolar macrophages activation during Mycobacterium tuberculosis infection Exp. Lung Res. (IF 1.5) Pub Date : 2022-09-14 Iris Selene Paredes-González, Omar Emiliano Aparicio-Trejo, Octavio Ramos-Espinosa, Manuel Othoniel López-Torres, Milena Maya-Hoyos, Monserrat Mendoza-Trujillo, Alejandra Barrera-Rosales, Dulce Mata-Espinosa, Juan Carlos León-Contreras, José Pedraza-Chaverri, Clara Espitia, Rogelio Hernández-Pando
ABSTRACT Purpose of the study: During the early and progressive (late) stages of murine experimental pulmonary tuberculosis, the differential activation of macrophages contributes to disease development by controlling bacterial growth and immune regulation. Mycobacterial proteins P27 and PE_PGRS33 can target the mitochondria of macrophages. This study aims to evaluate the effect of both proteins on
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Aerosol inhalation of Mycobacterium vaccae ameliorates airway structural remodeling in chronic asthma mouse model Exp. Lung Res. (IF 1.5) Pub Date : 2022-08-24 Qian-Nan Zhang, Huan Xiao, Li-Ting Fang, Qi-Xiang Sun, Lao-Dong Li, Si-Yue Xu, Chao-Qian Li
Abstract Background: Airway remodeling is accepted to be a determining component within the natural history of asthma. Nebulized inhalation of Mycobacterium vaccae (M. vaccae) has a protective effect on asthmatic mice. However, little is known regarding the effect of M. vaccae on airway structural remodeling in asthmatic mice. The purpose of this study was to explore the effect and the underlying mechanism
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Effect of using acoustic flo-tone training device and its smartphone application on enhancing inhalation technique from metered-dose inhaler with spacer in asthmatic children Exp. Lung Res. (IF 1.5) Pub Date : 2022-08-23 Sara M. Tony, Mona A. Abdelrahman, Mogeda Abd Elsalam, Mahmoud Sameer Shafik, Mohamed E. A. Abdelrahim
Abstract Aim of study: This research study aims to compare between two different counseling approaches; traditional verbal counseling vs. advanced counseling (in which we used the acoustic Flo-tone training device and its smartphone application combined with traditional verbal counseling) to determine the most beneficial counseling approach for asthmatic children who use metered-dose inhaler (MDI)
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Lef1 is transcriptionally activated by Klf4 and suppresses hyperoxia-induced alveolar epithelial cell injury Exp. Lung Res. (IF 1.5) Pub Date : 2022-08-11 Min Yang, Xueshan Huang, Fang Shen, Juanjuan Yi, Yanni Meng, Yanping Chen
Abstract Purpose Bronchopulmonary dysplasia (BPD) is a long-term respiratory condition. More than a quarter of extremely premature newborns are harmed by BPD. At present, there are no apparent effective drugs or treatments for the condition. In this study, we aimed to investigate the functional role and mechanism of lymphoid enhancer-binding factor 1 (Lef1) in BPD in vitro. Materials and Methods Blood
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BRD7 restrains TNF-α-induced proliferation and migration of airway smooth muscle cells by inhibiting notch signaling Exp. Lung Res. (IF 1.5) Pub Date : 2022-08-09 Hong Li, Tian Yang, Tianjun Chen, Ya Liu, Yamei Pang, Lan Yang
Abstract Objective: Bromodomain-containing protein 7 (BRD7) is a key component of the switch/sucrose non-fermentable complex that participates in chromatin remodeling and transcriptional regulation. Although the emerging role of BRD7 in the pathophysiology of various diseases has been observed, its role in asthma remains unknown. Here, we assessed the function of BRD7 as a mediator of airway remodeling
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Insulin-like growth factor-1 reduces hyperoxia-induced lung inflammation and oxidative stress and inhibits cell apoptosis through PERK/eIF2α/ATF4/CHOP signaling Exp. Lung Res. (IF 1.5) Pub Date : 2022-08-03 Haixia Cui, Shujian Zhang, Zhengxie Wu, Chunhua Xu, Dongyuan Xu, Zhengyong Jin
Abstract Background: Insulin-like growth factor-1 (IGF-1), a member of the insulin family, has a high degree of homology with insulin and exhibits anti-inflammatory and anti-oxidative stress properties. However, the potential protective effect of IGF-1 on hyperoxia-induced lung injury remains unknown. In this study, we aimed to explore the effects and mechanism of action of IGF-1 in hyperoxia-induced
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Low tidal volume ventilation alleviates ventilator-induced lung injury by regulating the NLRP3 inflammasome Exp. Lung Res. (IF 1.5) Pub Date : 2022-08-02 Lixia Wang, Jun Li, Yan Zhu, Binshan Zha
Abstract Purpose Low tidal volume ventilation (LTVV) is a well-known ventilation mode which can improve ventilator-induced lung injury (VILI). However, the mechanism of LTVV ameliorating VILI has not yet been elucidated. In this study, we aimed to reveal LTVV protected against VILI by inhibiting the activation of the NLRP3 inflammasome in bronchoalveolar lavage fluid (BALF) from humans and lungs from
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SRC3 deficiency exacerbates lipopolysaccharide-induced acute respiratory distress syndrome in mice Exp. Lung Res. (IF 1.5) Pub Date : 2022-08-02 Meixia Cui, Shengtong Guo, Ying Cui
Abstract Acute respiratory distress syndrome (ARDS) is a severe disease. Inflammation is the key element implicated in ARDS. Steroid receptor coactivator 3 (SRC3), a coactivator protein for transcription, is involved in regulation of inflammatory response. Here we explored the potential roles of SRC3 in ARDS. We utilized the SRC3 deficient (SRC3-/-) mice and established the lipopolysaccharides (LPS)-induced
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Downregulation of miR-761 ameliorates radiation-induced pulmonary fibrosis by regulating PGC-1α Exp. Lung Res. (IF 1.5) Pub Date : 2022-07-29 Zeng Wang, Junying Chen, Li Su, Jinsheng Hong
Abstract Background: Radiation-induced pulmonary fibrosis (RIPF) is a serious complication in patients treated with transthoracic irradiation. To date, there are no effective drugs for RIPF treatment. In this study, we attempted to explore the function of miR-761 in RIPF, further investigate its potential mechanism and evaluate its effectiveness in the treatment of RIPF. Methods: qRT-PCR analysis was
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Metabolic profile of exhaled breath condensate from the pneumonia patients Exp. Lung Res. (IF 1.5) Pub Date : 2022-06-16 Xiaoguang Li, Juan Du, Jing Chen, Fei Lin, Wei Wang, Ting-Ting Wei, Jie Xu, Qing-Bin Lu
Abstract Purpose of the Study Exhaled breath condensate (EBC) is increasingly being used for disease diagnosis and environmental exposure assessment as a noninvasive method reducing the risk of exposure. The purpose of this study was to investigate the application of a new sample type of EBC in pneumonia by metabolomics and to explore differential metabolites and potential metabolic pathways. Materials
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3,3′-Diindolylmethane attenuates inflammation and fibrosis in radiation-induced lung injury by regulating NF-κB/TGF-β/Smad signaling pathways Exp. Lung Res. (IF 1.5) Pub Date : 2022-05-20 Xia Zhou, Wu-An Bao, Xiang Zhu, Juan Lin, Ju-Fen Fan, Yang Yang, Xiang-Hui Du, Yue-Zhen Wang
Abstract Objective: This study aims to investigate the protective effect of 3,3′-diindolylmethane (DIM) on the radiation-induced lung injury (RILI) model and to explore its possible mechanism. Methods: A mouse model of RILI was established by thoracic irradiation, and dexamethasone was used as a positive drug to investigate the effect of DIM on RILI mice. Lung histopathology was analyzed by HE staining
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Collagen 3D matrices as a model for the study of cell behavior in pulmonary fibrosis Exp. Lung Res. (IF 1.5) Pub Date : 2022-05-20 Carlos Machahua, Vanesa Vicens-Zygmunt, Jesús Ríos-Martín, Roger Llatjós, Ignacio Escobar-Campuzano, María Molina-Molina, Ana Montes-Worboys
Abstract Purpose: Idiopathic pulmonary fibrosis (IPF) is a complex progressive chronic lung disease where epithelial to mesenchymal interaction, extracellular matrix (ECM) contact, and pro-fibrotic cytokines dynamics take part in the development of the disease. The study of IPF in the widespread in vitro two-dimensional (2 D) culture fails to explain the interaction of cells with the changing environment
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Effects of sulfur dioxide, ozone, and ambient air pollution on lung histopathology, oxidative-stress biomarkers, and apoptosis-related gene expressions in rats Exp. Lung Res. (IF 1.5) Pub Date : 2022-05-09 Sorayya Kheirouri, Dariush Shanehbandi, Monireh Khordadmehr, Mohammad Alizadeh, Fateme Eskandari Vaezi, Razieh Musapour Sultan Abad, Mehran Mesgari-Abbasi
Abstract Purpose of the Study Ambient air pollution (AAP) has become an important health problem globally. Besides, several pieces of evidence indicate that air pollutants such as sulfur dioxide (SO2) and ozone (O3) are major contributors to a wide range of non-communicable diseases. The present study investigated the effects of AAP, sulfur dioxide, and ozone on oxidative stress, histopathology, and
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Pulmonary inflammation caused by cigarette smoke combined with lipopolysaccharide up-regulated OATP2B1 in rat lung tissue and pulmonary epithelial cells Exp. Lung Res. (IF 1.5) Pub Date : 2022-04-20 Zihao Wang, Xin Fang, Shuyi Zhang, Jue Song
Abstract Organic anion transport polypeptide 2B1 (OATP2B1), as an uptake transporter, is involved in the transport of many related substrate drugs and endogenous substances in the lungs. A large amount of data shows that cigarette smoke plays an important role in the occurrence and development of lung diseases such as chronic obstructive pulmonary disease (COPD), asthma and bronchitis. However, the
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TGF-β-induced CCR8 promoted macrophage transdifferentiation into myofibroblast-like cells Exp. Lung Res. (IF 1.5) Pub Date : 2022-04-04 Haijun Liu, Qingzhou Guan, Peng Zhao, Jiansheng Li
Abstract Background: Idiopathic pulmonary fibrosis (IPF) is an interstitial disease of unknown origin, characterized by tissue fibrosis, for which currently there is no effective treatment. Macrophages, the main immune cells in lung tissue, are involved in the whole process of pulmonary fibrosis. In recent years, intercellular transformation has led to wide spread concern among pulmonary fibrosis researchers
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Correction Exp. Lung Res. (IF 1.5) Pub Date : 2022-03-15
(2022). Correction. Experimental Lung Research: Vol. 48, No. 2, pp. 100-101.
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Preliminary bronchodilator dose effect on aerosol-delivery through different nebulizers in noninvasively ventilated COPD patients Exp. Lung Res. (IF 1.5) Pub Date : 2022-03-02 Yasmin M. Madney, Hadeer S. Harb, Thierry Porée, Myriam Eckes, Marina E. Boules, Mohamed E. A. Abdelrahim
Abstract Objectives: This study aimed to evaluate the effect of a preliminary bronchodilator dose on the aerosol-d elivery by different nebulizers in noninvasively ventilated chronic obstructive pulmonary disease (COPD) patients. Method: COPD patients were randomized to receive study doses of 800 µg beclomethasone dipropionate (BPD) nebulized by either a vibrating mesh nebulizer (VMN) or a jet nebulizer
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Bronchial epithelial SIRT1 deficiency exacerbates cigarette smoke induced emphysema in mice through the FOXO3/PINK1 pathway Exp. Lung Res. (IF 1.5) Pub Date : 2022-02-08 Hui Jiang, Yaona Jiang, Yuanri Xu, Dong Yuan, Yaqing Li
Abstract Purpose: Cellular senescence and mitochondrial fragmentation are thought to be crucial components of the cigarette smoke(CS)-induced responses that contribute to the chronic obstructive pulmonary disease (COPD) development as a result of accelerated premature aging of the lung. Although there have been a few reports on the role of sirtuin 1(SIRT1) in mitochondrial homeostasis, senescence and
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Signal processing to remove spurious contributions to the assessment of respiratory mechanics Exp. Lung Res. (IF 1.5) Pub Date : 2021-12-22 Vitor Mori, Renato L. Vitorasso, Vitor A. Takeuchi, Wothan T. Lima, Maria A. Oliveira, Henrique T. Moriya
Abstract Aim: Signal disruptions in small animals during the realization of the Forced Oscillation Technique are a well-known cause of data loss as it leads to non-reliable estimations of the respiratory impedance. In this work, we assessed the effects of removing the disrupted epoch when a 3-seconds input signal composed of one and a half 2-seconds full cycle is used. Methods: We tested our hypothesis
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Effects of end-expiratory lung volume versus PaO2 guided PEEP determination on respiratory mechanics and oxygenation in moderate to severe ARDS Exp. Lung Res. (IF 1.5) Pub Date : 2021-12-26 Kazim Rollas, Pervin Hanci, Arzu Topeli
Abstract Background: There is no ideal method for determination of positive end-expiratory pressure (PEEP) in acute respiratory distress syndrome (ARDS) patients. We compared the effects of end-expiratory lung volume (EELV)-guided versus PaO2-guided PEEP determination on respiratory mechanics and oxygenation during the first 48 hours in moderate to severe ARDS. Methods: Twenty-two patients with moderate
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The 16S rRNA lung microbiome in mechanically ventilated patients: a methodological study Exp. Lung Res. (IF 1.5) Pub Date : 2021-12-28 Melanie Fromentin, Antoine Bridier-Nahmias, Jérôme Legoff, Severine Mercier-Delarue, Noémie Ranger, Constance Vuillard, Julien Do Vale, Noémie Zucman, Antonio Alberdi, Jean-Damien Ricard, Damien Roux
Abstract Purpose: Characterization of the respiratory tract bacterial microbiome is in its infancy when compared to the gut microbiota. To limit bias mandates a robust methodology. Specific amplification of the hypervariable (V) region of the 16SrRNA gene is a crucial step. Differences in accuracy exist for one V region to another depending on the sampled environment. We aimed to assess the impact
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Overexpression of bone morphogenetic protein receptor type 2 suppresses transforming growth factor β-induced profibrotic responses in lung fibroblasts Exp. Lung Res. (IF 1.5) Pub Date : 2022-01-17 Jun Fukihara, Suzanne Maiolo, Jessica Kovac, Koji Sakamoto, Keiko Wakahara, Naozumi Hashimoto, Paul N. Reynolds
Abstract Purpose of the study: Idiopathic pulmonary fibrosis (IPF) is a progressive disease with a poor prognosis. As the efficacy of currently available antifibrotics is limited, development of new therapies is warranted. Transforming growth factor (TGF)-β plays a central role in the pathogenesis of IPF through mechanisms such as promoting the production of extracellular matrix by fibroblasts. Conversely
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Cell cycle kinase CHEK2 in macrophages alleviates the inflammatory response to Staphylococcus aureus-induced pneumonia Exp. Lung Res. (IF 1.5) Pub Date : 2022-01-25 Fei Xie, Ruidong Chen, Jie Zhao, Chunyan Xu, Chunxiang Zan, Bin Yue, Wenqiu Tian, Wenxia Yi
Abstract Background Excessive macrophage-mediated inflammation participates in the development of Staphylococcus aureus (S. aureus)-induced pneumonia. Checkpoint kinase 2 (Chek2) was screened out as macrophage-related infantile pneumonia gene after the differentially expressed analysis of RNAseq data derived from pam3CSK4 stimulated bone marrow-derived macrophages (BMDMs). Methods RAW264.7 macrophage
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Transglutaminase 2 mediates lung inflammation and remodeling by transforming growth factor beta 1 via alveolar macrophage modulation Exp. Lung Res. (IF 1.5) Pub Date : 2021-11-24 Young Chan Kim, Jeonghyeon Kim, Subin Kim, Boram Bae, Ruth Lee Kim, Eui-Man Jeong, Sang-Heon Cho, Hye-Ryun Kang
Abstract Transforming growth factor beta 1 (TGF-β1) induces pulmonary fibrosis by enhancing epithelial apoptosis and affects the enzymatic activity of transglutaminase 2 (TG2). The aim of this study was to determine the role of TG2 in TGF-β1-induced lung remodeling and alveolar macrophage modulation. We characterized the in vivo effects of TGF-β1 and TG2 on lung inflammation, fibrosis, and macrophage
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Continuous positive airway pressure affects mitochondrial function and exhaled PGC1-α levels in obstructive sleep apnea Exp. Lung Res. (IF 1.5) Pub Date : 2021-11-11 Ching-Chi Lin, Wei-Ji Chen, Yi-Kun Sun, Chung-Hsin Chiu, Mei-Wei Lin, I-Shiang Tzeng
Abstract Purpose: Subjects with obstructive sleep apnea (OSA) exhibit systemic and upper airway oxidative stress and inflammation, which cause mitochondrial dysfunction. The intend of this study is to estimate mitochondrial function (mitochondrial DNA/nuclear DNA [Mt/N] ratio) and protein levels of peroxisome proliferator-coactivated receptor gamma co-activator 1-alpha (PGC1-α) in the exhaled breath