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Glugea plecoglossi (Strickland, 1911) infection in wild Sardinella aurita (Valenciennes, 1847) along the Campania coasts, in southern Italy J. Fish Dis. (IF 2.5) Pub Date : 2024-04-22 Maria Oliviero, Doriana Iaccarino, Emanuele Esposito, Gianluigi Paduano, Nicola D'Alessio, Giovanni Sgroi, Maria Gabriella Lucibelli, Clementina Auriemma, Barbara degli Uberti, Marianna D'Amore, Maria Dimatteo, Giovanna Fusco, Esterina De Carlo, Fabio Di Nocera
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Plasma and tissue transferrin and ferritin, and gene expression of ferritin, transferrin, and transferrin receptors I and II in channelcatfishIctalurus punctatusfed diets with different concentrations of inorganic or organic iron J. Fish Dis. (IF 2.5) Pub Date : 2024-04-15 Isaac Buyinza, Grace Ramena, Rebecca Lochmann, Amit Sinha, Michele Jones
Ferritin, transferrin, and transferrin receptors I and II play a vital role in iron metabolism, health, and indication of iron deficiency anaemia in fish. To evaluate the use of high‐iron diets to prevent or reverse channel catfish (Ictalurus punctatus) anaemia of unknown causes, we investigated the expression of these iron‐regulatory genes and proteins in channel catfish fed plant‐based diets. Catfish
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Rainbow trout (Oncorhynchus mykiss, Walbaum 1792) adenocarcinoma investigation with various diagnostic imaging techniques J. Fish Dis. (IF 2.5) Pub Date : 2024-04-08 Márton Hoitsy, György Hoitsy, János Gál, Árisz Ziszisz, Tamás Tóth, Endre Sós, Viktória Sós‐Koroknai, Csaba Jakab, Örs Petneházy, Tamás Donkó, Tamás Molnár, Miklós Marosán
Diagnostic imaging techniques provide a new aspect of the ante‐mortem and post‐mortem diagnostics in fish medicine. Ultrasonography, computed tomography (CT) and magnetic resonance imaging (MRI) can provide more information about the internal organs and pathognomic lesions. The authors used diagnostic imaging techniques to evaluate and describe the neoplastic malformation in a 3‐year‐old female rainbow
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Intestinal strangulation in farmed Atlantic cod (Gadus morhua): Pathological changes and possible predisposing anatomical features J. Fish Dis. (IF 2.5) Pub Date : 2024-04-08 Fredrik Strebel Skedsmo, Guro Løkka, Elvis Chikwati, Julianne Valla Jacobsen, Arild Espenes, Trond M. Kortner
During recent years, there has been a renewed interest in establishing farming of Atlantic cod (Gadus morhua) in Norway. However, a fatal abdominal disorder compromises animal welfare and causes economic losses. A similar problem was present during a previous attempt to establish Atlantic cod farming more than a decade ago. In this paper, we provide the first in‐depth description of this intestinal
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Outbreak of trypanosomiasis in cage‐cultured large yellow croaker in China J. Fish Dis. (IF 2.5) Pub Date : 2024-04-03 Pan Qin, Xingfu Chen, Bilian Lou, Tong Wu, Jingsong Yang, Weicheng Wang, Guangliang Huang, Xinhua Chen
CONFLICT OF INTEREST STATEMENT The authors do not have financial and non-financial competing interests.
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Hepatic transcriptome profiling of largemouth bass (Micropterus salmoides Lacépède) injected with Flavobacterium covae or lipopolysaccharide J. Fish Dis. (IF 2.5) Pub Date : 2024-04-01 Nilima N. Renukdas, Anita M. Kelly, Gaurav Zinta, Amit Kumar Sinha
Flavobacterium covae (columnaris) is the most detrimental bacterial disease affecting the largemouth bass (Micropterus salmoides Lacépède) aquaculture industry. In the current study, fish received an intraperitoneal injection of either 1× PBS (100 μL), LPS in PBS (100 μL, 10 μg/mL), or F. covae (100 μL, 2.85 × 1011 CFU/mL) to simulate immunological challenges. After 24 h post‐injection, liver tissue
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Most of the escaped farmed salmon entering a river during a 5‐year period were infected with one or more viruses J. Fish Dis. (IF 2.5) Pub Date : 2024-03-31 Abdullah S. Madhun, Egil Karlsbakk, Øystein Skaala, Monica F. Solberg, Vidar Wennevik, Alison Harvey, Sonnich Meier, Per T. Fjeldheim, Kaja Christine Andersen, Kevin A. Glover
Disease interactions between farmed and wild populations have been poorly documented for most aquaculture species, in part due to the complexities to study this. Here, we tested 567 farmed Atlantic salmon escapees, captured in a Norwegian river during 2014–2018, for five viral infections that are prevalent in global salmonid aquaculture. Over 90% of the escapees were infected with one or more viruses
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Comparative transcriptome analysis explored the molecular mechanisms of a luxR‐type regulator regulating intracellular survival of Aeromonas hydrophila J. Fish Dis. (IF 2.5) Pub Date : 2024-03-31 Weiqin Chen, Leilei Mao, Qingpi Yan, Lingmin Zhao, Lixing Huang, Jiaonan Zhang, Yingxue Qin
Aeromonas hydrophila is not a traditional intracellular bacterium. However, previous studies revealed that pathogenic A. hydrophila B11 could temporarily survive for at least 24 h in fish phagocytes, and the regulation of intracellular survival in bacteria was associated with regulators of the LuxR‐type. The mechanisms of luxR08110 on the A. hydrophila's survival in macrophages were investigated using
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Expression profiles of heat shock protein 70 and high‐mobility groups box‐1 protein in gnotobiotic brine shrimp challenged with different virulence levels of isogenic Vibrio harveyi strains J. Fish Dis. (IF 2.5) Pub Date : 2024-03-31 Nguyen Thi Xuan Hong, Kartik Baruah, Hung Nguyen Van, Daisy Vanrompay, Peter Bossier
CONFLICT OF INTEREST STATEMENT The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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Comparative pathogenicity of Nocardia seriolae in Nile tilapia (Oreochromis niloticus), milkfish (Chanos chanos) and Asian seabass (Lates calcarifer) J. Fish Dis. (IF 2.5) Pub Date : 2024-03-25 Sandra Celenia Nazareth, Li‐Wu Cheng, Pei‐Chi Wang, Shih‐Chu Chen
Nocardiosis, caused by Nocardia seriolae, has been a prominent disease in Southeast Asian aquaculture in the last three decades. This granulomatous disease reported in various fish species is responsible for significant economic losses. This study investigated the pathogenicity of N. seriolae in three cultured species in Taiwan: Nile tilapia (omnivore), milkfish (herbivore) and Asian seabass (carnivore)
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Inactivated Aeromonas salmonicida impairs adaptive immunity in lumpfish (Cyclopterus lumpus) J. Fish Dis. (IF 2.5) Pub Date : 2024-03-25 Setu Chakraborty, Hajarooba Gnanagobal, Ahmed Hossain, Trung Cao, Ignacio Vasquez, Danny Boyce, Javier Santander
Aeromonas salmonicida, a widely distributed aquatic pathogen causing furunculosis in fish, exhibits varied virulence, posing challenges in infectious disease and immunity studies, notably in vaccine efficacy assessment. Lumpfish (Cyclopterus lumpus) has become a valuable model for marine pathogenesis studies. This study evaluated several antigen preparations against A. salmonicida J223, a hypervirulent
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Comparative proteome analysis revealed potential biomarkers and the underlying immune mechanisms in Vibrio‐resistant hybrid grouper, Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂ J. Fish Dis. (IF 2.5) Pub Date : 2024-03-25 Nurhikmah Abu Aziz, Annie Christianus, Wan Mohd Syazwan Wan Solahudin, Intan Safinar Ismail, Chen‐Fei Low
Vibrio alginolyticus is the causative agent of vibriosis, a common bacterial infection in grouper aquaculture that is associated with the development of haemorrhagic and non‐haemorrhagic ulcerations on the fish. In the present study, comparative proteome analysis was performed on serum samples from Vibrio‐resistant and Vibrio‐susceptible grouper. Samples were analysed using high‐throughput LC‐MS/MS
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Water‐in‐oil adjuvant challenges in fish vaccination: An experimental inactivated adjuvanted vaccine against betanodavirus infection in Senegalese sole J. Fish Dis. (IF 2.5) Pub Date : 2024-03-25 Yulema Valero, Sandra Souto, José G. Olveira, Carmen López‐Vázquez, Carlos P. Dopazo, Isabel Bandín
The extensive growth of intensive fish farming has led to a massive spread of infectious diseases. Nervous necrosis virus (NNV) is the causative agent of the viral encephalo‐ and retinopathy disease which has become a major threat for fish farming all over the globe. The devastating mortality rates recorded in disease outbreaks, especially when infected specimens are at early stages of development
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Review of quorum‐quenching probiotics: A promising non‐antibiotic‐based strategy for sustainable aquaculture J. Fish Dis. (IF 2.5) Pub Date : 2024-03-25 Anisa Rilla Lubis, Md Afsar Ahmed Sumon, Nguyen Dinh‐Hung, Arun K. Dhar, Jérôme Delamare‐Deboutteville, Do‐Hyung Kim, Andrew P. Shinn, Duangkhaetita Kanjanasopa, Patima Permpoonpattana, Hien Van Doan, Nguyen Vu Linh, Christopher L. Brown
The emergence of antibiotic‐resistant bacteria (ARBs) and genes (ARGs) in aquaculture underscores the urgent need for alternative veterinary strategies to combat antimicrobial resistance (AMR). These measures are vital to reduce the likelihood of entering a post‐antibiotic era. Identifying environmentally friendly biotechnological solutions to prevent and treat bacterial diseases is crucial for the
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Enterolith with a bonefish spine nidus in a Brazilian cownose ray Rhinoptera brasiliensis (Müller & Henle) (Myliobatiformes: Rhinopteridae) J. Fish Dis. (IF 2.5) Pub Date : 2024-03-16 L. O. Camilo, B. L. Maganhe, H. Gallo Neto, E. G. Sanches
An adult female Brazilian cownose ray (Rhinoptera brasiliensis), housed in Ubatuba Aquarium died after loss of appetite period. During necropsy, an enterolith was discovered partially obstructing the intestinal lumen. Examination of the enterolith revealed a bonefish spine nidus. Enterolithiasis has been linked to multiple factors including diet, genetics, alkaline intestinal environments and, as in
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Shewanella xiamenensis‐associated ulcerative dermatitis in koi carp (Cyprinus rubrofuscus) J. Fish Dis. (IF 2.5) Pub Date : 2024-03-16 Andrew F. Rich, Mark Naguib
Ulcerative dermatitis (UD) is common in ornamental fish collections and is typically associated with a wide range of bacterial aetiologies. Clinical reports describing Shewanella xiamenensis‐associated UD are limited, however, despite growing attention to pathogenic Shewanella species in fish. Two out of 95 koi carp with UD were presented for clinical assessment by a commercial collection (n = 3000
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Clinical symptoms and histopathological changes in coho salmon affected by the erythrocytic inclusion body syndrome (EIBS) are caused by the infection of piscine orthoreovirus 2 (PRV‐2) J. Fish Dis. (IF 2.5) Pub Date : 2024-03-14 Tomokazu Takano, Satoshi Miwa, Tomomasa Matsuyama, Ikunari Kiryu, Miho Honjo, Takamitsu Sakai, Yuta Matsuura, Masatoshi Yamasaki, Akira Kumagai, Chihaya Nakayasu
The relationship of histopathological changes and the infection of Piscine orthoreovirus 2 (PRV‐2) was investigated in coho salmon that were suffering from the erythrocytic inclusion body syndrome (EIBS). Immunohistochemical observations revealed abundant σ1 protein of PRV‐2 in the spongy layer of the ventricle of the heart, where severe myocarditis was observed. In the spleen, the virus protein was
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Expression of immuno‐transcriptome response in red hybrid tilapia (Oreochromis sp.) hindgut following vaccination with feed‐based bivalent vaccine J. Fish Dis. (IF 2.5) Pub Date : 2024-03-14 Nur Shidaa Mohd Ali, Mohamad Syazwan Ngalimat, Mohd Zamri Saad, Mohammad Noor Amal Azmai, Annas Salleh, Zarirah Zulperi, Ina Salwany Md Yasin
Streptococcosis and aeromoniasis are the main obstacles to sustainable tilapia production. Vaccination offered an effective method to control microbial infections. Previously, a feed‐based bivalent vaccine (FBBV) containing killed whole organisms of Streptococcus agalactiae and Aeromonas hydrophila mixed with 10% palm oil was successfully developed, which provided good protection against streptococcosis
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Novel Aquareovirus isolated from channel catfish (Ictalurus punctatus) used in mussel restoration efforts in Wisconsin J. Fish Dis. (IF 2.5) Pub Date : 2024-03-11 Eric Leis, Ryan Katona, Sara Dziki, Rebekah McCann, Isaac Standish, Eryn Opgenorth, Audrey Dikkeboom, Ailam Lim, Charlotte E. Ford, Tony L. Goldberg
Channel catfish (Ictalurus punctatus) are a food fish extensively reared in aquaculture facilities throughout the world and are also among the most abundant wild catfish species in North America, making them a popular target of anglers. Furthermore, channel catfish are important members of aquatic ecosystems; for example, they serve as a glochidial host for the endangered winged mapleleaf mussel (Quadrula
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Diagnosis, isolation and description of a novel amnoonvirus recovered from diseased fancy guppies, Poecilia reticulata J. Fish Dis. (IF 2.5) Pub Date : 2024-03-05 Esteban Soto, Benjamin R. LaFrentz, Susan Yun, Dorothea Megarani, Eileen Henderson, Chutchai Piewbang, Amber E. Johnston, Somporn Techangamsuwan, Terry Fei Fan Ng, Janet Warg, Win Surachetpong, Kuttichantran Subramaniam
The guppy, Poecilia reticulata, is one of the most common cultured ornamental fish species, and a popular pet fish highly desired by hobbyists worldwide due to its availability of many brilliantly coloured fish of many varieties. The susceptibility of guppies to diseases presents a remarkable concern for both breeders and hobbyists. In this study, we report the emergence of disease in fancy guppies
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In vitro effects of phytogenic feed additive on Piscirickettsia salmonis growth and biofilm formation J. Fish Dis. (IF 2.5) Pub Date : 2024-02-29 Natacha Santibáñez, Matías Vega, Tatiana Pérez, Ricardo Enriquez, Carla Estefanía Escalona, Cristian Oliver, Alex Romero
Piscirickettsiosis is the main cause of mortality in salmonids of commercial importance in Chile, which is caused by Piscirickettsia salmonis, a Gram‐negative, γ‐proteobacteria that can produce biofilm as one of its virulence factors. The Chilean salmon industry uses large amounts of antibiotics to control piscirickettsiosis outbreaks, which has raised concern about its environmental impact and the
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First report of Metschnikowia bicuspidata infection in the oriental river prawn (Macrobrachium nipponense, de Haan) in China J. Fish Dis. (IF 2.5) Pub Date : 2024-02-29 Yingyue Yu, Songyue You, Chengcheng Feng, Xiaodong Li, Yuenan Xing, Qingbiao Hu, Jie Bao, Hongbo Jiang
During breeding, some oriental river prawns (Macrobrachium nipponense, de Haan), an important aquaculture species in China, exhibit yellowish‐brown body colouration, reduced appetite, and vitality. Diseased prawns revealed characteristic emulsifying disease signs, including whitened musculature, hepatopancreatic tissues, milky haemolymph, and non‐coagulation. The present study investigated the causative
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Potential to grow carp oedema virus (genogroup I) in monolayers of carp‐derived primary cells with further implication in cell analysis J. Fish Dis. (IF 2.5) Pub Date : 2024-02-29 Ehdaa Eltayeb Eltigani Abdelsalam, Zuzana Bláhová, Ali Asghar Baloch, Veronika Piačková
Carp oedema virus (CEV) has distinct molecularly identified genogroups of viral mutations, denoted as I, IIa, and IIb. Failure to propagate CEV in vitro limits studies towards understanding its interactions with host cells. Here, virus isolates belonging to genogroup I collected during natural outbreaks in the Czech Republic were employed for routine CEV cultivation in monolayers of carp‐derived primary
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Serine protease inhibitor 3 (Serpin3) from Penaeus vannamei selectively interacts with Vibrio parahaemolyticusPirAvp J. Fish Dis. (IF 2.5) Pub Date : 2024-02-26 Thanh‐Nguyen Le, Thuan‐Thien Dinh, Thuy‐Dung Mai‐Hoang, Ebrahim Razzazi‐Fazeli, Hieu Tran‐Van
Acute Hepatopancreatic Necrosis Disease (AHPND) represents a significant challenge in the field of shrimp aquaculture. This disease is primarily caused by Vibrio parahaemolyticus strains harbouring the pVA1 plasmid encoding the PirAvp and PirBvp toxins. To combat this epidemic and mitigate its devastating consequences, it is crucial to identify and characterize the receptors responsible for the binding
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Identification of new amoebae strains in rainbow trout (Oncorhynchus mykiss, Walbaum) farms affected by nodular gill disease (NGD) in Northeastern Italy J. Fish Dis. (IF 2.5) Pub Date : 2024-02-24 Ginevra Brocca, Alessandro Truant, Hana Peckova, Martina Lisnerová, Alberto Perolo, Marialetizia Fioravanti, Ivan Fiala, Gianfranco Gabai, Francesco Quaglio, Andrea Gustinelli
Nodular gill disease (NGD) is an emerging condition associated with amoeba trophozoites in freshwater salmonid farms. However, unambiguous identification of the pathogens still must be achieved. This study aimed to identify the amoeba species involved in periodic NGD outbreaks in two rainbow trout (Oncorhynchus mykiss) farms in Northeastern Italy. During four episodes (February–April 2023), 88 fish
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Unveiling the positive impact of biofloc culture on Vibrio parahaemolyticus infection of Pacific white shrimp by reducing quorum sensing and virulence gene expression and enhancing immunity J. Fish Dis. (IF 2.5) Pub Date : 2024-02-19 Widanarni Widanarni, Muhamad Gustilatov, Julie Ekasari, Pande Gde Sasmita Julyantoro, Diana Elizabeth Waturangi, Sukenda Sukenda
This study aimed to evaluate and unveil the positive impact of biofloc culture on Vibrio parahaemolyticus infection of Pacific white shrimp by reducing quorum sensing (QS) and virulence gene expression and enhancing shrimp's immunity. The shrimp with an average body weight of 0.50 ± 0.09 g were reared in containers with a volume of 2.5 L, 21 units, and a density of 20 shrimp L−1. The shrimp were cultured
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Comparative phenotype and transcriptome analysis revealed the role of ferric uptake regulator (Fur) in the virulence of Vibrio harveyi isolated from diseased American eel (Anguilla rostrata) J. Fish Dis. (IF 2.5) Pub Date : 2024-02-19 Qijuan Wan, Shaowei Zhai, Minxia Chen, Ming Xu, Songlin Guo
Vibrio harveyi is commonly found in salt and brackish water and is recognized as a serious bacterial pathogen in aquaculture worldwide. In this study, we cloned the ferric uptake regulator (fur) gene from V. harveyi wild‐type strain HA_1, which was isolated from diseased American eels (Anguilla rostrata) and has a length of 450 bp, encoding 149 amino acids. Then, a mutant strain, HA_1‐Δfur, was constructed
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Rapid and sensitive detection of large yellow croaker iridovirus by real-time RPA and RPA-LFD J. Fish Dis. (IF 2.5) Pub Date : 2024-02-13 Xiaoru Liu, Yong Cao, Jiayin Wang, Suyuheng Cao, Liqun Lu, Yousheng Jiang
Large yellow croaker (Larimichthys crocea) is a vital marine-cultured species in China. Large yellow croaker iridovirus (LYCIV) can cause a high mortality rate in L. crocea. Rapid and convenient detection of LYCIV is an urgent demand for diagnosis. In this study, rapid and simple recombinase polymerase amplification (RPA), real-time RPA and RPA combined with lateral flow dipstick (RPA-LFD) methods
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Establishment of a real-time PCR for the detection of decapod iridescent virus 1 (DIV1) J. Fish Dis. (IF 2.5) Pub Date : 2024-02-01 Xiao-Meng Guo, Jing-Yi Xing, Anqi Li, Liang Qiu, Qing-Li Zhang, Jie Huang
CONFLICT OF INTEREST STATEMENT All authors declare that there is no conflict of interest or financial interest.
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Phytochemical analysis and antibacterial activity of palm waste extract against Vibrio harveyi and Vibrio parahaemolyticus J. Fish Dis. (IF 2.5) Pub Date : 2024-02-01 Nasmia Nasmia, Novalina Serdiati, Akbar Marzuki Tahya, Muhammad Safir
Vibrio harveyi and Vibrio parahaemolyticus are species of the Vibrio genus that often cause disease and mass mortality in crustaceans. If not handled quickly and appropriately, these diseases can cause considerable losses to farmers. Therefore, it is necessary to find a solution with safe and environmentally friendly disease prevention technology using natural ingredients, among others from plants
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Investigation of extragonadal teratomas in two Poecilia wingei J. Fish Dis. (IF 2.5) Pub Date : 2024-01-30 Denis V. Prazdnikov, Ekaterina A. Kondakova
Teratoma is a rare tumour in fish consisting of tissues from more than one germ layer, that may be located in either the gonads or extragonadal sites. Teratomas in many fish species remain poorly understood. In this work, we performed the first histological examinations of extragonadal teratomas in Poecilia wingei and also examined the influence of a large teratoma on male sexual activity. The studied
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Development and characterization of brain cell line from Trachinotus blochii and its application in virological and gene expression studies J. Fish Dis. (IF 2.5) Pub Date : 2024-01-29 A. Nafeez Ahmed, S. Mithra, S. Suryakodi, G. Taju, M. J. Abdul Wazith, K. Kanimozhi, V. Rajkumar, A. Badhusha, S. Abdul Majeed, A. S. Sahul Hameed
A permanent cell line, SPB (Snubnose pompano brain) was established from Trachinotus blochii by the explant culture method. It has been sub-cultured more than 75 passages and showed optimal growth at 28°C using L-15 medium supplemented with 15% to 20% FBS. The SPB cells were cryopreserved at different passage levels for various applications. SPB cells were composed of fibroblastic and epithelial-like
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Pathogenic bacteria prevalence in cultured Nile tilapia in Southwest Mexico: A real-time PCR analysis J. Fish Dis. (IF 2.5) Pub Date : 2024-01-25 Sonia A. Soto-Rodriguez, Francis I. Marrujo Lopez, Karla G. Aguilar-Rendon, Rafael Hernández Guzmán
The present study investigates molecular-based PCR techniques to estimate the prevalence of fish pathogens in southwest Mexico where recurrent mortality in the tilapia cultures has been observed. Sample of internal organs and lesions of Nile tilapia were taken and analysed in 2018, 2019, 2020 and 2022 to detect bacterial pathogens using PCR. No samples were taken in 2021 due to the COVID-19 pandemic
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Dimethomorph activity and its effect on morphology in different oomycete species of economic and veterinary interest J. Fish Dis. (IF 2.5) Pub Date : 2024-01-24 Irene Maja Nanni, Perla Tedesco, David Baldo, Roberta Galuppi, Marina Collina
CONFLICT OF INTEREST STATEMENT The authors declare no conflict of interest.
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First report and pathogenicity of Vibrio campbellii (VCAHPND) isolated in South Korea J. Fish Dis. (IF 2.5) Pub Date : 2024-01-24 Hee-Jae Choi, Da-Yeon Choi, Ji-Hoon Lee, Jun-Hwan Kim, Yue Jai Kang
CONFLICT OF INTEREST STATEMENT The authors declare no conflict of interest to declare.
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Bacterial abscesses in whitespotted bamboo sharks (Chiloscyllium plagiosum) in managed care J. Fish Dis. (IF 2.5) Pub Date : 2024-01-19 Alexandra K. Mason, Lance Adams, Michael M. Garner
Sixteen whitespotted bamboo sharks (Chiloscyllium plagiosum) with histologically similar bacterial abscesses were submitted to Northwest ZooPath from nine zoo and aquarium institutions over a 17-year period. These abscesses were characterized by inflammatory cell infiltrates and necrosis with intralesional small, Gram-positive, acid-fast negative, cocci bacteria. The clinical presentation, histologic
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Non-lethal detection of Eubothrium crassum (Cestoda) in farmed Atlantic salmon, Salmo salar, using anal swabs and real-time PCR J. Fish Dis. (IF 2.5) Pub Date : 2024-01-18 Haakon Hansen, Bjørn Spilsberg, Sigmund Sevatdal, Trine Sakariassen, Christoph Hahn, Saima Nasrin Mohammad, Egil Karlsbakk
Detection of intestinal parasites in fish typically requires autopsy, resulting in the sacrifice of the fish. Here, we describe a non-lethal method for detecting the tapeworm Eubothrium crassum in fish using anal swabs and real-time PCR detection. Two assays were developed to detect cytochrome oxidase I (COI) mitochondrial DNA and 18S ribosomal DNA sequences of E. crassum, respectively. The assays
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Tail Fan Necrosis syndrome in decapod crustaceans: A review J. Fish Dis. (IF 2.5) Pub Date : 2024-01-16 John Brian Jones, Cara L. Brosnahan, Anjali Pande
Lobsters and crayfish in Australasia can develop a condition known as Tail Fan Necrosis (TFN syndrome). Many attempts have been made to find a primary pathogen or link the syndrome to commercial activities, but a solution remains elusive. TFN syndrome is a ‘wicked problem’, a problem difficult or impossible to solve because of incomplete and contradictory information forming a matrix of potential outcomes
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Synergistic interaction of nanoparticles and probiotic delivery: A review J. Fish Dis. (IF 2.5) Pub Date : 2024-01-16 Srirengaraj Vijayaram, Hary Razafindralambo, Yun Zhang Sun, Giuseppe Piccione, Cristiana Roberta Multisanti, Caterina Faggio
Nanotechnology is an expanding and new technology that prompts production with nanoparticle-based (1–100 nm) organic and inorganic materials. Such a tool has an imperative function in different sectors like bioengineering, pharmaceuticals, electronics, energy, nuclear energy, and fuel, and its applications are helpful for human, animal, plant, and environmental health. In exacting, the nanoparticles
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Coinfection of channel catfish (Ictalurus punctatus) with virulent Aeromonas hydrophila and Flavobacterium covae exacerbates mortality J. Fish Dis. (IF 2.5) Pub Date : 2024-01-12 Allison L. Wise, Benjamin R. LaFrentz, Anita M. Kelly, Mark R. Liles, Matt J. Griffin, Benjamin H. Beck, Timothy J. Bruce
Flavobacterium covae and virulent Aeromonas hydrophila are prevalent bacterial pathogens within the US catfish industry that can cause high mortality in production ponds. An assessment of in vivo bacterial coinfection with virulent A. hydrophila (ML09-119) and F. covae (ALG-00-530) was conducted in juvenile channel catfish (Ictalurus punctatus). Catfish were divided into seven treatments: (1) mock
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Establishment of a gill cell line from yellowfin seabream (Acanthopagrus latus) for studying Amyloodinium ocellatum infection of fish J. Fish Dis. (IF 2.5) Pub Date : 2024-01-13 Zhicheng Li, Jingyu Zhuang, Jiaming Chen, Jizhen Cao, Qing Han, Zhi Luo, Baotun Wang, Hebing Wang, Anxing Li
Amyloodinium ocellatum is among the most devastating protozoan parasites, causing huge economic losses in the mariculture industry. However, the pathogenesis of amyloodiniosis remains unknown, hindering the development of targeted anti-parasitic drugs. The A. ocellatum in vitro model is an indispensable tool for investigating the pathogenic mechanism of amyloodiniosis at the cellular and molecular
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Isolation, identification and characterization of Aeromonas jandaei from diseased Chinese soft-shell turtles J. Fish Dis. (IF 2.5) Pub Date : 2024-01-13 Mengmeng Chen, Mingyang Xue, Jingtao Chen, Zidong Xiao, Xiaowei Hu, Chunjie Zhang, Nan Jiang, Yuding Fan, Yan Meng, Yong Zhou
Aeromonas jandaei is a gram-negative bacterium commonly found in aquatic environments and can induce illnesses in amphibians, reptiles and aquatic animals. In this study, a strain of bacteria was isolated from the diseased Chinese soft-shell turtle (Pelodiscus sinensis), then named strain JDP-FX. This isolate was identified as A. jandaei after analysis of morphological, physiological and biochemical
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A novel defensin-like peptide C-13326 possesses protective effect against multidrug-resistant Aeromonas schubertii in hybrid snakehead (Channa maculate ♀ × Channa argus ♂) J. Fish Dis. (IF 2.5) Pub Date : 2024-01-10 Huanrong He, Xinzhi Huang, Caiyi Wen, Chun Liu, Biao Jiang, Yanhua Huang, Youlu Su, Wei Li
The purpose of this study was to investigate whether a defensin-like antimicrobial peptide (C-13326 peptide) identified in Hermetia illucens could possess protective effect against multidrug-resistant Aeromonas schubertii in hybrid snakehead (Channa maculate ♀ × Channa argus ♂). The cDNA of C-13326 peptide comprised 243 nucleotides encoding 80 amino acids, with six conserved cysteine residues and the
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Mixed Eimeria and Cryptosporidium infection and its effects on pathology and clinical outcomes in juvenile Asian seabass (Lates calcarifer) cultured in Thailand J. Fish Dis. (IF 2.5) Pub Date : 2024-01-07 Watcharapol Suyapoh, Narissara Keawchana, Peerapon Sornying, Sirikachorn Tangkawattana, Pokphon Khirilak, Sasibha Jantrakajorn
Coccidiosis is an important disease in juvenile fish because of severe intestinal injury during infection. We first reported the mixed infection of intestinal coccidia and its association with health status and pathological findings in juvenile Asian seabass (Lates calcarifer) cultured in Thailand. Two groups of Asian seabass, 60-day fish and 90-day fish, were sampled to investigate prevalence and
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Natural product honokiol exhibits antiviral effects against Micropterus salmoides rhabdovirus (MSRV) both in vitro and in vivo J. Fish Dis. (IF 2.5) Pub Date : 2024-01-08 Fei Yang, Bin Yang, Kaige Song, Yingjie Jin, Gaoxue Wang, Pengfei Li, Qing Yu, Fei Ling
Micropterus salmoides rhabdovirus (MSRV) is a formidable pathogen, presenting a grave menace to juvenile largemouth bass. This viral infection frequently leads to epidemic outbreaks, resulting in substantial economic losses within the aquaculture industry. Unfortunately, at present, there are no commercially available vaccines or pharmaceutical treatments to combat this threat. In order to address
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Influence of probiotic and prebiotic supplementation on intestinal microbiota and resistance to Edwardsiella ictaluri infection in channel catfish (Ictalurus punctatus) following florfenicol administration J. Fish Dis. (IF 2.5) Pub Date : 2023-12-28 Caitlin E. Older, Matt J. Griffin, Bradley M. Richardson, Geoffrey C. Waldbieser, J. Grant Reifers, Penelope M. Goodman, Cynthia Ware, Delbert M. Gatlin, David J. Wise, Fernando Y. Yamamoto
Enteric septicemia of catfish (ESC), caused by the gram-negative enteric bacteria Edwardsiella ictaluri, is a significant threat to catfish aquaculture in the southeastern United States. Antibiotic intervention can reduce mortality; however, antibiotic use results in an imbalance, or dysbiosis, of the gut microbiota, which may increase susceptibility of otherwise healthy fish to enteric infections
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Study of the frequencies of erythrocyte abnormalities as in situ biomarkers of genotoxic risk of chemicals in special fish stock in water supply reservoirs J. Fish Dis. (IF 2.5) Pub Date : 2023-12-27 Jiří Řehulka, Jaroslav Bradík
In three water-supply reservoirs in the catchment area of the Odra River (Czech Republic), a special fish stock was monitored for control of health to estimate the mutagenic effect of chemicals. The results contribute to obtaining initial information about the morphology of erythrocyte abnormalities classified in 21 categories in 16 fish species in reservoirs with abundant salmonids (the Morávka Reservoir)
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Molecular quantification of parasitic sea louse larvae depends on species and life stage J. Fish Dis. (IF 2.5) Pub Date : 2023-12-25 Nathan E. Mertz, Rachel A. Paterson, Bengt Finstad, Hege Brandsegg, Ida Pernille Øystese Andersskog, Frode Fossøy
Sea lice cause substantial economic and environmental harm to Norway's aquaculture industry and wild salmonid populations. Rapid, accurate quantification of lice larval densities in coastal waters remains the greatest bottleneck for providing empirical data on infestation risk within wild salmon habitats and aquaculture production regions. We evaluated the capability of droplet digital PCR (ddPCR)
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Basibranchial structure affecting cardiac morphology in Atlantic salmon (Salmo salar L.) J. Fish Dis. (IF 2.5) Pub Date : 2023-12-25 Trygve T. Poppe, Anne Katrine Reed, Helene Wisløff
CONFLICT OF INTEREST STATEMENT The authors declare no conflicts of interest.
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Effect of temperature on the protective efficacy of a live attenuated vaccine against herpesviral haematopoietic necrosis in goldfish J. Fish Dis. (IF 2.5) Pub Date : 2023-12-19 Hiroaki Saito, Shungo Minami, Manami Yuguchi, Aiko Shitara, Hidehiro Kondo, Goshi Kato, Motohiko Sano
The live attenuated vaccine P7-P8 strain against herpesviral haematopoietic necrosis, which is caused by cyprinid herpesvirus 2 (CyHV-2), exhibits high protective efficacy in goldfish at 25°C, the predominant temperature for this disease; however, the effect of water temperature during the vaccination period on efficacy has not been determined. In this study, an in vitro experiment revealed that the
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Development and application of a recombinase-aided amplification combined with a lateral flow dipstick assay for rapid visual detection of anguillid herpesvirus 1 J. Fish Dis. (IF 2.5) Pub Date : 2023-12-19 Xi Chen, Hua Chen, Jun-Qing Ge
Eel (Anguilla sp.) is an important freshwater-cultured species with high economic value in China. Anguillid herpesvirus 1 (AngHV-1) has been proven to be the pathogen of “mucus sloughing and haemorrhagic septicaemia disease” in eels, resulting in significant mortality and substantial losses to the eel industry. Current diagnostic methods for detecting AngHV-1 are limited to laboratory-based tests,
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Diversity of fungus-like stramenopilous organisms (Oomycota) in Lithuanian freshwater aquaculture: Morphological and molecular analysis, risk to fish health J. Fish Dis. (IF 2.5) Pub Date : 2023-12-12 Svetlana Markovskaja, Reda Iršėnaitė, Audrius Kačergius, Gintarė Sauliutė, Milda Stankevičiūtė
The present work is the first comprehensive study of fungus-like stramenopilous organisms (Oomycota) diversity in Lithuanian fish farms aimed at proper identification of saprolegniasis pathogens, which is important for water quality control, monitoring infection levels and choosing more effective treatments for this disease in aquaculture. Pathogenic to fish, Saprolegnia and other potentially pathogenic
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Confirmatory test of active IHHNV infection in shrimp by immunohistochemistry and IHHNV-LongAmp PCR J. Fish Dis. (IF 2.5) Pub Date : 2023-12-10 Supapong Imsonpang, Arnon Pudgerd, Charoonroj Chotwiwatthanakun, Jiraporn Srisala, Piyachat Sanguanrut, Chanadda Kasamechotchung, Kallaya Sritunyalucksana, Suparat Taengchaiyaphum, Rapeepun Vanichviriyakit
The presence of endogenous viral elements (EVE) in the penaeid shrimp genome has been recently reported and suggested to be involved in the host recognition of viral invaders. Our previous report of a search for EVE of infectious hypodermal and haematopoietic necrosis virus (IHHNV-EVE) in the Thai Penaeus monodon whole genome sequence project (GenBank accession no. JABERT000000000) confirmed the presence
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Establishment of a TaqMan probe-based qPCR assay for detecting microsporidia Enterospora epinepheli in grouper J. Fish Dis. (IF 2.5) Pub Date : 2023-12-07 Quan-lin Liu, Yuan Wang, Jie Chen, Guo-qing Pan, Yan-Feng Yue, Ze-Yang Zhou, Wen-hong Fang
Enterospora epinepheli is an intranuclear microsporidian parasite causing serious emaciative disease in hatchery-bred juvenile groupers (Epinephelus spp.). Rapid and sensitive detection is urgently needed as its chronic infection tends to cause emaciation as well as white faeces syndrome and results in fry mortality. This study established a TaqMan probe-based real-time quantitative PCR assays targeting
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Identification of a potential antigen stimulating immune response against Vibrio parahaemolyticus infection in hybrid tilapia (Oreochromis aureus♂ × Oreochromis niloticus♀) J. Fish Dis. (IF 2.5) Pub Date : 2023-12-08 Qingsong Zeng, Yunxiao Sun, Peifang Lai, Mingqin Huang, Ximing Peng, Junjie Huang, Qintao Chen, Yanxu Chen, Huaqian Wang
Vibrio parahaemolyticus (V. parahaemolyticus) is a major pathogen that causes substantial losses in the marine fishery. With the emergence of antibiotic resistance, vaccines have become the most effective approach against V. parahaemolyticus infection. Adhesion factors on the cell surface are pivotal in the colonization and pathogenesis of V. parahaemolyticus within the host, highlighting their potential
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Disruption of a kasB homolog gene (kasB) causes attenuation of cell invasion and virulence of Nocardia seriolae J. Fish Dis. (IF 2.5) Pub Date : 2023-12-06 Su-Ming Zhou, Feng-Ling Shu, Hao Liu, Dong Qian, Jia-Song Xie, Ya-Jun Wang, Fei Yin
Nocardia seriolae is the primary aetiological agent of nocardiosis in fish, which causes mass mortality in freshwater and marine fish. β-ketoacyl-ACP synthase (KAS) is one of the essential enzymes in the synthesis of mycolic acids (MASs) in Mycobacterium spp. and has been chosen as the target for therapeutic intervention in mycobacterial diseases. In the present study, a kasB homologue gene (kasB)
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Magnetic resonance imaging shows spinal curvature in Chinook salmon (Oncorhynchus tshawytscha) is associated with chronic inflammation of peri-vertebral soft tissues J. Fish Dis. (IF 2.5) Pub Date : 2023-12-06 Bailey A. Lovett, Elwyn C. Firth, Matthew R. Perrott, John S. Munday, Beau P. Pontre, Anna-Maria P. Lydon, Jane E. Symonds, Mark A. Preece, Neill A. Herbert
Chinook salmon (Oncorhynchus tshawytscha) farmed in New Zealand are known to develop abnormal spinal curvature late in seawater production. Its cause is presently unknown, but there is evidence to suggest a neuromuscular pathology. Using magnetic resonance imaging (MRI), we evaluated the relationship between soft tissue pathology and spinal curvature in farmed Chinook salmon. Regions of interest (ROIs)
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Iron supplementation in the diets of hybrid catfish (Ictalurus punctatus × I. furcatus) juveniles affected haematocrit levels and potentially decreased disease resistance to Edwardsiella ictaluri J. Fish Dis. (IF 2.5) Pub Date : 2023-12-01 Fernando Y. Yamamoto, Matt J. Griffin, Bradley M. Richardson, Justin M. Stilwell, Nicholas Romano, Penelope M. Goodman, J. Grant Reifers, David J. Wise
To prevent catfish idiopathic anaemia, diets fortified with iron have been adopted as a regular practice on commercial catfish farms to promote erythropoiesis. However, the effects of prolonged exposure of excess dietary iron on production performance and disease resistance for hybrid catfish (Ictalurus punctatus × I. furcatus) remains unknown. Four experimental diets were supplemented with ferrous