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In silico approach uncovers the shared genetic landscape of type 2 diabetes mellitus and asthenozoospermia Syst. Biol. Reprod. Med. (IF 2.1) Pub Date : 2024-09-18 Yinwei Chen, Taotao Sun, Chang Liu, Longjie Gu, Penghui Yuan
Asthenozoospermia (AZS) is one of the most common types of male infertility. Current evidence revealed that type 2 diabetes mellitus (T2DM) is closely associated with declining semen quality, espec...
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Reversible effects of the SARS-CoV-2 on semen parameters Syst. Biol. Reprod. Med. (IF 2.1) Pub Date : 2024-09-01 Meritxell Jodar, Yasmina Barral, Marina Leiva, Judit Castillo, Ruth Barrio, Inés Agustí, Aina Borràs, Pilar Carrillo, Roger Matheu, Raquel Ferreti, Julio Herrero, Pilar Reimundo, Jessica Navero-Castillejos, Gemma Casals, Marta Guimerà, Juan Manuel Corral, Mikel Martinez, Melchor Carbonell, Lluís Bassas, Dolors Manau, Rafael Oliva
Despite that the SARS-CoV-2 pandemic has been controlled, it has affected a large proportion of the population, raising some concerns about potential sequelae in men at reproductive age. To contrib...
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Bacteriospermia in men among infertile couples in the Nepalese population Syst. Biol. Reprod. Med. (IF 2.1) Pub Date : 2024-08-21 Anima Shrestha, Dev Raj Joshi, Dijan Vaidya, Sanu Maiya Shrestha, Anjana Singh
Infection of the male urogenital tract or male accessory glands is considered one of the important causes of male infertility, and results in the presence of bacteria in semen affecting the fertili...
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Transcriptome analyses reveal key features of mouse seminal vesicle during aging Syst. Biol. Reprod. Med. (IF 2.1) Pub Date : 2024-08-21 Peng Luo, Haibin Guo, Baoning Liu, Zhiqiang Zhang, Yun Xie, Jiahui Yao, Xiangping Li, Jun Bian, Jintao Zhuang, Bin Ouyang, Jinhua Wu
Despite the significant morphological changes that occur in the seminal vesicles with aging, the transcriptomic characteristics remain largely unexplored. To address this, we performed bulk RNA seq...
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Classification of idiopathic recurrent spontaneous miscarriage using FTIR and Raman spectroscopic fusion technology Syst. Biol. Reprod. Med. (IF 2.1) Pub Date : 2024-08-16 Dadoma Sherpa, Chiranjib Bhowmick, Tummala Pavan, Dhruva Abhijit Rajwade, Sumana Halder, Imon Mitra, Sunita Sharma, Pratip Chakraborty, Sanjukta Dasgupta, Koel Chaudhury
Recurrent spontaneous miscarriage refers to the repeated loss of two or more clinically detected pregnancies occurring within 24 weeks of gestation. No identifiable cause has been identified for ne...
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What sperm parameters effect blastocyst formation and quality during ICSI with severe male infertility. Syst. Biol. Reprod. Med. (IF 2.1) Pub Date : 2024-07-16 Weihua Jiang,Lihua Zhu,Lina Gao,Jie Wang,Junshun Fang,Linjun Chen,Shanshan Wang
Sperm-derived genetic material contributes half of the genome to the embryo, hence it's crucial to investigate which sperm parameter influences blastocyst formation in the intracytoplasmic sperm injection (ICSI) cycles with severe male infertility. The retrospective study analyzed 296 ICSI cycles with severe oligoasthenoteratozoospermia (OAT) and 99 ICSI cycles with preimplantation genetic testing
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Identification of potential differences in salivary proteomic profiles between estrus and diestrus stage of estrous cycle in dairy cows Syst. Biol. Reprod. Med. (IF 2.1) Pub Date : 2024-07-15 Arsha Shaji, Arumugam Kumaresan, Manish Kumar Sinha, Pradeep Nag, Shivanagouda Patil, Sakthivel Jeyakumar, Vedamurthy Gowdar Veerappa, Ayyasamy Manimaran, Kerekoppa Ramesha
In the present study, a comparative global high-throughput proteomic analysis strategy was used to identify proteomic differences between estrus and diestrus stage of estrous cycle in dairy cows. S...
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The formation of aggregated chromatin/chromosomes in mouse oocytes treated with high concentration of IBMX as a model for a chromosome transfer in human Syst. Biol. Reprod. Med. (IF 2.1) Pub Date : 2024-07-07 Wei Xiao, Sakura Akao, Ryota Okamoto, Junko Otsuki
The presence of cyclic adenosine monophosphate (cAMP) has been considered to be a fundamental factor in ensuring meiotic arrest prior to ovulation. cAMP is regarded as a key molecule in the regulat...
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piRNA expression patterns in high vs. low fertility bovine sperm Syst. Biol. Reprod. Med. (IF 2.1) Pub Date : 2024-06-26 Nicholas Werry, Stewart J. Russell, Raamkumaar Sivakumar, Sarah Miller, Katie Hickey, Steven Larmer, Michael Lohuis, Clifford Librach, Jonathan LaMarre
PIWI-interacting RNAs (piRNAs) are 24–32 nucleotide RNA sequences primarily expressed in germ cells and developing embryos that suppress transposable element expression to protect genomic integrity...
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Novel bioinformatic analyses of somatic cell contamination in sperm samples Syst. Biol. Reprod. Med. (IF 2.1) Pub Date : 2024-06-22 Carter Norton, Chad Pollard, Kelaney Stalker, Kenneth Aston, Timothy Jenkins
The assessment of epigenetic profiles in sperm is sensitive to somatic cell contamination, which can influence methylation signals at gene promoters. This contamination is particularly problematic ...
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Correlation between sperm DNA fragmentation and methylation in male partners of couples with idiopathic recurrent pregnancy loss Syst. Biol. Reprod. Med. (IF 2.1) Pub Date : 2024-06-24 Delna Irani, Deepti Tandon, Vandana Bansal, Anushree Patil, Nafisa Balasinor, Dipty Singh
With ∼50% recurrent pregnancy loss cases being termed idiopathic (iRPL), understanding of contribution of male factors to iRPL is still lacking. Higher prevalence of sperm DNA fragmentation index (...
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Association of lifestyle and occupational exposure factors with human semen quality: a cross-sectional study of 1060 participants Syst. Biol. Reprod. Med. (IF 2.1) Pub Date : 2024-06-19 Wen Yang, Zongliu Duan, Guanjian Li, Hao Geng, Yang Gao, Qunshan Shen, Liting Liu, Guanxiong Wang, Xiaomin Zha, Chuan Xu, Ping Zhou, Bing Song, Dongdong Tang, Huan Wu, Zhaolian Wei, Feng Tang, Xiaojin He
The incidence of male infertility (MI) is rising annually. However, the lifestyle and occupational exposure factors contributing to MI remain incompletely understood. This study explored the effect...
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Study of the genomics and transcriptomics profiles of male-infertility genes in human prostate cancer: an in silico analysis Syst. Biol. Reprod. Med. (IF 2.1) Pub Date : 2024-06-13 Farima Said Ali-Samani, Arman Shahrisa, Maryam Tahmasebi-Birgani, Mohammadreza Hajjari, Pegah Ghandil
The World Health Organization has considered the infertility as an international public health problem. Infertility affect nearly 1 in 7 couples and male component contributes to 50% of infertility...
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Active immunization with recombinant GnRH6-CRM197 inhibits reproductive function of male rats Syst. Biol. Reprod. Med. (IF 2.1) Pub Date : 2024-06-04 XinBao Gong, Xu Yan, MengXian Li, MoYan Di, JunTai Lu, ShuangShuang Xu, ZhiHao Pan, YanYun Zhu, ZhuoYa Wu, Wei Zhang, Ping Qin, Ya Liu, YunSheng Li, FuGui Fang
Gonadotropin-releasing hormone (GnRH) vaccines have been successfully used for the inhibition of gonadal development and function, but current GnRH-based vaccines often present variability in the r...
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Moderate concentration of Lactobacillus metabolites does not adversely affect mouse sperm Syst. Biol. Reprod. Med. (IF 2.1) Pub Date : 2024-06-04 Katarzyna Kotarska
Many couples in contemporary societies suffer from infertility of unexplained origins (idiopathic). A promising treatment strategy within this context involves the administration to women of prepar...
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FOXA1 co-activates circODC1 and ODC1 in HPV-positive cervical cancer cell growth Syst. Biol. Reprod. Med. (IF 2.1) Pub Date : 2024-05-14 Rong Jin, Hongfang Li, Shoushan Nan, Huiju Wang
As demonstrated in previous research, hsa_circ_0052602 (circODC1) is dynamically expressed in HPV-positive cervical cancer (CC). CircODC1 expression was quantified using qRT-PCR, and its role in CC...
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Correlation between standard sperm parameters and sperm DNA fragmentation from 11,339 samples Syst. Biol. Reprod. Med. (IF 2.1) Pub Date : 2024-04-17 Tingting Yang, Lin Yu, Jinyan Xu, Lijuan Ying, Yelin Jia, Yan Zheng, Bin Zhou, Fuping Li
Conventional semen parameters have long been considered fundamental in male fertility analyses. However, doubts have been raised regarding the clinical utility of the assessment of spermatozoa (spe...
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Prediction of deleterious non-synonymous SNPs of human MDC1 gene: an in silico approach Syst. Biol. Reprod. Med. (IF 2.1) Pub Date : 2024-04-17 Vaishnavee Thote, Susha Dinesh, Sameer Sharma
MDC1 (Mediator of DNA damage Checkpoint protein 1) functions to facilitate the localization of numerous DNA damage response (DDR) components to DNA double-strand break sites. MDC1 is an integral co...
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Insight into the complexity of male infertility: a multi-omics review Syst. Biol. Reprod. Med. (IF 2.1) Pub Date : 2024-03-22 Rebeka Podgrajsek, Alenka Hodzic, Martin Stimpfel, Tanja Kunej, Borut Peterlin
Male infertility is a reproductive disorder, accounting for 40–50% of infertility. Currently, in about 70% of infertile men, the cause remains unknown. With the introduction of novel omics and adva...
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Biostimulatory effects of boar seminal gel, saliva and semen on sexual behavior of young boars, gilts and sows Syst. Biol. Reprod. Med. (IF 2.1) Pub Date : 2024-03-05 Sunil Kumar, Rafiqul Islam, Anesha Chanda, Pranab Jyoti Das, Santanu Banik, Keshab Barman, Seema Rani Pegu, Swaraj Rajkhowa, Vivek Kumar Gupta
The present study aimed to identify novel biostimulatory compounds in boar seminal gel (SG), saliva and semen using Gas chromatography-mass spectrometry (GC-MS). The bio-stimulatory effect of SG, S...
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Effects of first and second division modes on euploidy acquisition in human embryo Syst. Biol. Reprod. Med. (IF 2.1) Pub Date : 2024-03-01 Yamato Mizobe, Yukari Kuwatsuru, Yuko Kuroki, Yumiko Fukumoto, Mari Tokudome, Harue Moewaki, Tokiko Iwakawa, Kazuhiro Takeuchi
The aim of this study was to non-invasively investigate euploid embryos using methods other than pre-implantation genetic testing for aneuploidy. The study focused on direct cleavage (DC) observed ...
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Engineered exosome as a biological nanoplatform for drug delivery of Rosmarinic acid to improve implantation in mice with induced endometritis Syst. Biol. Reprod. Med. (IF 2.1) Pub Date : 2024-02-07 Morteza Taravat, Reza Asadpour, Razi Jafari Jozani, Amir Fattahi, Monireh Khordadmehr, Hamed Hajipour
Endometritis is an inflammatory and histopathologic disease in uterine tissues that interferes with the proper decidualization and implantation of the embryo. In this study, rosmarinic acid (RA) is...
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Preimplantation genetic testing as a preventive strategy for the transmission of mitochondrial DNA disorders Syst. Biol. Reprod. Med. (IF 2.1) Pub Date : 2024-02-07 Xinpeng Lan, Wu Liji Ao, Ji Li
Mitochondrial diseases are distinct types of metabolic and/or neurologic abnormalities that occur as a consequence of dysfunction in oxidative phosphorylation, affecting several systems in the body...
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Hydroxycitric acid and capsaicin combination alleviates obesity-induced testicular apoptosis, oxidative stress and inflammation Syst. Biol. Reprod. Med. (IF 2.1) Pub Date : 2024-02-07 V. V. Sathibabu Uddandrao, Seshathri Eraniappan, Asokan Balakrishnan Ramajayam, Sengottuvelu Singaravel, Anitha Roy, Brahma Naidu Parim, Chandrasekaran Ponnusamy, Saravanan Ganapathy, Ponmurugan Ponnusamy, Vadivukkarasi Sasikumar
Recent research in rodents suggests that oxidative stress, inflammation, and apoptosis in the testes caused by high-fat diets (HFD) are a cause of male infertility. To investigate the therapeutic e...
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E-SBiRM Syst. Biol. Reprod. Med. (IF 2.1) Pub Date : 2024-01-10 Stephen A. Krawetz
Published in Systems Biology in Reproductive Medicine (Vol. 70, No. 1, 2024)
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Identification of ferroptotic genes and phenotypes in idiopathic nonobstructive azoospermia Syst. Biol. Reprod. Med. (IF 2.1) Pub Date : 2023-11-20 Chen Liao, Tian-Wen Peng, Xiao-Min Li, Zhi-Cong Chen, Mu-Ye Wang, Xin Ye, Yu Lan, Xin Fu, Geng An
Effective treatments for nonobstructive azoospermia (NOA), which affects 1% of all men globally, are limited by undefined pathogenic mechanisms, especially in idiopathic NOA (iNOA). Here, we tried ...
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Polyamines modulate mouse sperm motility Syst. Biol. Reprod. Med. (IF 2.1) Pub Date : 2023-11-20 Lorena Rodríguez-Páez, Charmina Aguirre-Alvarado, Germán Chamorro-Cevallos, Alcántara-Farfán Veronica, Calderón-Espinosa Sandra Irel, Castillo-Pérez Hugo, Carlos Armando García-Pérez, Guadalupe Elizabeth Jiménez-Gutiérrez, Joaquín Cordero-Martínez
Polyamines are polycationic molecules which contains two or more amino groups (-NH3+) highly charged at physiological pH, and among them we found spermine, spermidine, putrescine, and cadaverine. T...
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Pharmacological investigation of vitamin E with combined oral contraceptives on INHBA gene against PCOS that intricate through melatonin PKC pathway Syst. Biol. Reprod. Med. (IF 2.1) Pub Date : 2023-11-20 Rukaiah Fatma Begum, Sumithra Mohan
The most prevalent endocrine and metabolic condition in women of reproductive age are polycystic ovary syndrome (PCOS) with significant risk factors such as circadian rhythm and melatonin disruptio...
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MEHP activates JNK to inhibit the migration of human foreskin fibroblasts Syst. Biol. Reprod. Med. (IF 2.1) Pub Date : 2023-11-20 Hu Zhang, Xuan Yi, Wei Hu, Guoqiang Zhu, Xiaowen Fu, Wei Jin, Long Qin, Mingyong Li
This study aimed to investigate the impact of mono(2-ethylhexyl) phthalate (MEHP) on the proliferation, apoptosis, and migration of human foreskin fibroblast cells (HFF-1) and the role of the JNK s...
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Correspondence: Interpreting live birth rates following preimplantation genetic testing for aneuploidy: per embryo transferred or initiated cycle* Syst. Biol. Reprod. Med. (IF 2.1) Pub Date : 2023-11-01 Pedro Augusto Araújo Monteleone, Tatiana Carvalho de Souza Bonetti
Published in Systems Biology in Reproductive Medicine (Vol. 69, No. 5, 2023)
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Response to Correspondence re: Live birth per embryo transfer with next generation sequencing preimplantation genetic testing: an analysis of 26,107 cycles* Syst. Biol. Reprod. Med. (IF 2.1) Pub Date : 2023-11-01 Papri Sarkar, Anthony N. Imudia
Published in Systems Biology in Reproductive Medicine (Vol. 69, No. 5, 2023)
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Prevalence of human papilloma virus and Chlamydia trachomatis in endometrial and cervical carcinoma: a comparative study in North Indian women Syst. Biol. Reprod. Med. (IF 2.1) Pub Date : 2023-09-05 Heena Gautam, Sumita Mehta, Nidhi Nayar, Neha Kumar, Syed Akhtar Husain, Mausumi Bharadwaj
Cervical cancer (Cacx) is the second and endometrial cancer (Ec) is the third most common gynecological cancer worldwide. The present study aims to understand the complex and unexplored conditions ...
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Studying the effect of hyperoside on recovery from cyclophosphamide induced oligoasthenozoospermia Syst. Biol. Reprod. Med. (IF 2.1) Pub Date : 2023-08-14 Qigang Fan, Ruifen He, Yi Li, Pu Gao, Runchun Huang, Rong Li, Jiayu Zhang, Hongli Li, Xiaolei Liang
Oligoasthenozoospermia is becoming a serious problem, but effective prevention or treatment is lacking. Hyperoside, one of the main active ingredients in traditional Chinese medicine, may be effect...
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Effects of NGS-based PGT-a for idiopathic recurrent pregnancy loss and implantation failure: a retrospective cohort study Syst. Biol. Reprod. Med. (IF 2.1) Pub Date : 2023-07-17 Xiao Shi, Youyong Tang, Chenxin Liu, Weiyu Li, Hui Lin, Wenqi Mao, Min Huang, Qingjun Chu, Liantong Wang, Song Quan, Chengming Xu, Qiang Ma, Jinliang Duan
To clarify the effect of next-generation sequencing (NGS)–based preimplantation genetic testing for aneuploidy (PGT-A) combined with trophectoderm (TE) biopsy on the pregnancy outcomes of idiopathi...
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A rare ring chromosome 21 abnormality is associated with azoospermia in two different phenotypically normal cases Syst. Biol. Reprod. Med. (IF 2.1) Pub Date : 2023-07-04 Ezgi Gizem Berkay, Birsen Karaman, Seher Başaran
Azoospermia can be diagnosed with spermiogram analysis, and karyotyping is the golden standard to explain the etiology. In this study, we investigated two male cases with azoospermia and male infer...
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Live birth per embryo transfer with next generation sequencing preimplantation genetic testing: an analysis of 26,107 cycles Syst. Biol. Reprod. Med. (IF 2.1) Pub Date : 2023-06-02 Papri Sarkar, Erika P. New, Rachel G. Sprague, Robert Stillman, Eric Widra, Samad Jahandideh, Kate Devine, Anthony N. Imudia
Abstract The technique and platform used for preimplantation genetic testing for aneuploidy (PGT-A) have undergone significant changes over time. The contemporary technique utilizes trophectoderm biopsy followed by next-generation sequencing (NGS). The goal of this study was to explore the role of PGT-A using NGS technique exclusively in contemporary in vitro fertilization (IVF) practice. For this
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Relationship among seminal antigenicity, antioxidant status and metabolically active sperm from Holstein-Friesian (Bos taurus) bulls Syst. Biol. Reprod. Med. (IF 2.1) Pub Date : 2023-05-24 Santhanahalli Siddalingappa Archana, Divakar Swathi, Laxman Ramya, Hulliyurdurga Shameeulla Heena, Balaganur Krishnappa, Bala Krishnan Binsila, Duraisamy Rajendran, Sellappan Selvaraju
Sperm antigenicity has been implicated as a regulatory factor for acquiring fertilizing competence in the female reproductive tract. Overt immune response against the sperm proteins leads to idiopa...
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Naringenin ameliorates aluminum toxicity-induced testicular dysfunctions in mice by suppressing oxidative stress and histopathological alterations Syst. Biol. Reprod. Med. (IF 2.1) Pub Date : 2023-05-19 Ravina Rai, Deepali Jat, Siddhartha Kumar Mishra
Environmental aluminum intoxication has shown increasingly alarming negative consequences on reproductive health. This needs mechanistic exploration and preventive management using medicines like h...
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The impact of cryopreservation on both sperm HPV-negative and positive subtypes Syst. Biol. Reprod. Med. (IF 2.1) Pub Date : 2023-05-13 Maria Anagnostou, Maria Samara, Eleni Thodou, Christina I. Messini, Konstantinos Dafopoulos, Katerina Chatzimeletiou, Eleni Dovolou, Alexandros Daponte, George Koukoulis, George Anifandis
Abstract It is well known that various human papillomavirus (HPV) genotypes are present in semen specimens. Also, it has been demonstrated that sperm parameters are negatively affected when HPV infection is present in the sperm sample. Besides all these, the effect of cryopreservation on HPV sensitivity and resistance is not known. The aim of the present study is to evaluate first the prevalence of
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Comparison of microbial profiles and viral status along the vagina-cervix-endometrium continuum of infertile patients Syst. Biol. Reprod. Med. (IF 2.1) Pub Date : 2023-04-27 Mark Jain, Elena Mladova, Anna Dobychina, Karina Kirillova, Anna Shichanina, Daniil Anokhin, Liya Scherbakova, Larisa Samokhodskaya, Olga Panina
Abstract For decades, the endometrium was considered to be a sterile environment. However, now this concept is disputed, and there is growing evidence that microbiota composition might affect endometrial receptivity. Routine clinical management of infertility is still limited to a microbiological assessment of the lower reproductive tract. The purpose of this study was to compare the abundance of various
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Exploring the internal exposome of seminal plasma with semen quality and live birth: A Pilot Study Syst. Biol. Reprod. Med. (IF 2.1) Pub Date : 2023-04-25 Emily Houle, YuanYuan Li, Madison Schroder, Susan L McRitchie, Tayyab Rahil, Cynthia K Sites, Susan Jenkins Sumner, J. Richard Pilsner
Abstract Infertility is clinically defined as the inability to achieve pregnancy within 12 months of regular unprotected sexual intercourse and affects 15% of couples worldwide. Therefore, the identification of novel biomarkers that can accurately predict male reproductive health and couples’ reproductive success is of major public health significance. The objective of this pilot study is to test whether
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The use of in silico extreme pathway (ExPa) analysis to identify conserved reproductive transcriptional-regulatory networks in humans, mice, and zebrafish Syst. Biol. Reprod. Med. (IF 2.1) Pub Date : 2023-04-06 David Hala
Abstract Vertebrate sex determination and differentiation are coordinated by the activations and maintenance of reproductive transcriptional-regulatory networks (TRNs). There is considerable interest in studying the conserved design principles and functions of reproductive TRNs given that their intricate regulation is susceptible to disruption by gene mutations or exposures to exogenous endocrine disrupting
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LncRNA PSMG3-AS1 is upregulated in prostate carcinoma and downregulates miR-106b through DNA methylation Syst. Biol. Reprod. Med. (IF 2.1) Pub Date : 2023-04-06 Liansheng Zhang, Yougan Chen, Zhenjie Wang, Qiang Xia
Abstract Long non-coding RNA PSMG3-AS1 is known to play critical roles in several types of cancer, while its role in prostate carcinoma (PC) is unknown. This study aimed to explore the involvement of PSMG3-AS1 in PC. In this study, RT-qPCR analysis showed that PSMG3-AS1 was upregulated, while miR-106b was downregulated in PC. PSMG3-AS1 and miR-106b were inversely and significantly correlated across
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Rewiring of miRNA-mRNA bipartite co-expression network as a novel way to understand the prostate cancer related players Syst. Biol. Reprod. Med. (IF 2.1) Pub Date : 2023-04-05 Mohammad Mehdi Naghizadeh, Behnaz Bakhshandeh, Farshid Noorbakhsh, Marjan Yaghmaie, Ali Masoudi-Nejad
Abstract The differential expression and direct targeting of mRNA by miRNA are two main logics of the traditional approach to constructing the miRNA-mRNA network. This approach, could be led to the loss of considerable information and some challenges of direct targeting. To avoid these problems, we analyzed the rewiring network and constructed two miRNA-mRNA expression bipartite networks for both normal
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In vitro effects of plasma rich in growth factors on human teratozoospermic semen samples Syst. Biol. Reprod. Med. (IF 2.1) Pub Date : 2023-03-15 Hanieh Ghasemian Nafchi, Yaser Azizi, Fatemehsadat Amjadi, Iman Halvaei
Abstract There is a correlation between teratozoospermia and production of reactive oxygen species leading to poor assisted reproductive techniques outcomes. This study aimed to examine the effect of plasma-rich in growth factors (PRGF) on teratozoospermic samples. Twenty-five teratozoospermic samples were included in this study. After sperm preparation, it was divided into four groups, including 0
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A transcriptomic insight into the human sperm microbiome through next-generation sequencing Syst. Biol. Reprod. Med. (IF 2.1) Pub Date : 2023-03-10 Celia Corral-Vazquez, Joan Blanco, Riccardo Aiese Cigliano, Sarrate Zaida, Francesca Vidal, Ester Anton
Abstract The purpose of this study is to provide novel information through Next Generation Sequencing (NGS) for the characterization of viral and bacterial RNA cargo of human sperm cells from healthy fertile donors. For this, RNA-seq raw data of poly(A) RNA from 12 sperm samples from fertile donors were aligned to microbiome databases using the GAIA software. Species of viruses and bacteria were quantified
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Focus on centrin in normal and altered human spermatozoa Syst. Biol. Reprod. Med. (IF 2.1) Pub Date : 2023-03-09 Elena Moretti, Daria Noto, Roberta Corsaro, Giulia Collodel
Abstract This review provides details on the role of centrin in human spermatozoa and in various forms of male infertility. Centrin is a calcium (Ca2+)-binding phosphoprotein that is located in the centrioles – which are typical structures of the sperm connecting piece and play a key role in centrosome dynamics during sperm morphogenesis – as well as in zygotes and early embryos during spindle assembly
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Transcriptomic analysis of the Non-Obstructive Azoospermia (NOA) to address gene expression regulation in human testis Syst. Biol. Reprod. Med. (IF 2.1) Pub Date : 2023-03-08 Govindkumar Balagannavar, Kavyashree Basavaraju, Akhilesh Kumar Bajpai, Sravanthi Davuluri, Shruthi Kannan, Vasan S. Srini, Darshan S. Chandrashekar, Neelima Chitturi, Kshitish K. Acharya
Abstract There is a need to understand the molecular basis of testes under Non-Obstructive Azoospermia (NOA), a state of failed spermatogenesis. There has been a lack of attention to the transcriptome at the level of alternatively spliced mRNAs (iso-mRNAs) and the mechanism of gene expression regulation. Hence, we aimed to establish a reliable iso-mRNA profile of NOA-testes, and explore molecular mechanisms
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Chronic stress decreases fertility parameters in female rats Syst. Biol. Reprod. Med. (IF 2.1) Pub Date : 2023-02-27 Fahiel Casillas, Alejandra Flores-González, Lizbeth Juárez-Rojas, Alma López, Miguel Betancourt, Eduardo Casas, Iván Bahena, Edmundo Bonilla, Socorro Retana-Márquez
Abstract Multiple effects of stress on health have been reported; however, reproductive alterations in oocytes and cumulus cells have not been fully described. In females, chronic stress has been shown to produce alterations in the estrous cycle, to decrease oocyte in vivo maturation, and to increase the percentage of abnormal oocytes. The aim of this study was to evaluate whether the oocytes from
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Intra-individual variation of sperm DNA fragmentation in the Human ejaculate Syst. Biol. Reprod. Med. (IF 2.1) Pub Date : 2023-02-22 Mercedes González-Martínez, Carmen López-Fernández, Pascual Sánchez-Martín, Stephen D. Johnston, Jaime Gosálvez
Abstract This retrospective study assessed the biological intra-individual variability of the percentage of sperm with DNA damage (SDF) observed in subsequent ejaculates of the same individual. Variation in SDF was analyzed using the Mean Signed Difference (MSD) statistic based on 131 individuals, comprising 333 ejaculates. Either two, three or four ejaculates were collected from each individual. With
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Optimization of human semen analysis using CASA-Mot technology Syst. Biol. Reprod. Med. (IF 2.1) Pub Date : 2023-02-20 Almudena García-Molina, Nuria Navarro, Anthony Valverde, Sara Sadeghi, Nicolás Garrido, Carles Soler
Abstract The purpose of this study is to investigate the optimal framerate (FR) and the use of different counting chambers for improving CASA-Mot technology use in Andrology. Images were captured at 500 fps, then segmented and analyzed in several ranges of FRs (from 25 to 250) to define the asymptotic point that as an optimal FR. This work was replicated using counting chambers based in capillarity
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Oxytocin modulates steroidogenesis-associated genes and estradiol levels in the placenta Syst. Biol. Reprod. Med. (IF 2.1) Pub Date : 2023-02-14 Sung-Min An, Min Jae Kim, Jea Sic Jeong, So Young Kim, Da Som Kim, Beum-Soo An, Seung Chul Kim
Abstract Oxytocin (OXT) plays a significant role during pregnancy, especially toward the end of pregnancy. Some studies have reported that OXT is involved in the stimulation of steroidogenesis in several organs. However, the effects of OXT on placental steroidogenesis have not yet been established. In this study, we investigated the regulation of steroid hormones and steroidogenic enzymes by OXT-associated
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The effect of ionomycin-induced oocyte activation on multiple morphological abnormalities of the sperm flagella Syst. Biol. Reprod. Med. (IF 2.1) Pub Date : 2023-02-11 Zhiren Liu, Yujia Guo, Xingting Chen, Chen Lin, Xinxin Guo, Mingting Jiang, Qicai Liu
Abstract Artificial oocyte activation (AOA) is considered an effective method to improve clinical outcomes in patients with some forms of male factor infertility and does not increase the risk of birth defects. However, the effects of AOA on patients with multiple morphological abnormalities of the sperm flagella (MMAF) caused by a DNAH1 mutation are still unknown. To explore the effects, our study
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Superior mesenteric ganglion via ovarian plexus nerve involved in the cross-talk between noradrenaline and GnRH in rat ovaries Syst. Biol. Reprod. Med. (IF 2.1) Pub Date : 2023-01-23 María Belén Delsouc, Sandra Vallcaneras, Cristina Daneri Becerra, Fabián Heber Mohamed, Marina Fernández, Adriana Soledad Vega Orozco, Marilina Casais
Abstract There is evidence of the existence of an intraovarian gonadotropin-releasing hormone (GnRH) system. There are also reports about the influence of extrinsic ovarian innervation in gonadal function. Therefore, it is interesting to study the relationship between ovarian sympathetic innervation and GnRH to shed light on possible physiological and pathophysiological implications. This work aimed
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The New Year of Systems Biology in Reproductive Medicine Syst. Biol. Reprod. Med. (IF 2.1) Pub Date : 2023-01-13 Stephen A. Krawetz
Published in Systems Biology in Reproductive Medicine (Vol. 69, No. 1, 2023)
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Forecasting most deleterious nsSNPs in human TLR9 gene and their cumulative impact on biophysical features of the protein using in silico approaches Syst. Biol. Reprod. Med. (IF 2.1) Pub Date : 2023-01-12 Heena Gautam, Ved Vrat Verma, Syed Akhtar Husain, Mausumi Bharadwaj
Abstract In women, the uterine cervix and corpus uteri are two main suspects, playing a major role in cancer-associated-mortality. Immunologically, Toll-like receptors (TLRs) associated with the innate immune system, can recognize pathogens and induce immune responses against pathogens. Cellularly, TLR9 expression occurs in immune system cells including macrophages, natural killer cells, dendritic
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The age-related required number of zygotes estimated from prior clinical studies of preimplantation genetic testing for aneuploidy (PGT-A) Syst. Biol. Reprod. Med. (IF 2.1) Pub Date : 2023-01-09 Tasuku Mariya, Takeshi Sugimoto, Takema Kato, Toshiaki Endo, Hiroki Kurahashi
Abstract Women who are undergoing preimplantation genetic testing for aneuploidy (PGT-A) often wish to know how many eggs will be required to optimize the chances of a live birth. However, no precise data on this can yet be provided during genetic counseling for this procedure. On the basis of PGT-A data from related studies and current databases, we have estimated that the number of zygotes required
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Long non-coding RNA DNMBP-AS1 promotes prostate cancer development by regulating LCLAT1 Syst. Biol. Reprod. Med. (IF 2.1) Pub Date : 2023-01-05 Xiangang Yin, Suying Wang, Rong Ge, Jinping Chen, Youliang Gao, Shanshan Xu, Ting Yang
Abstract Prostate cancer (PCa) is as a serious threat to male’s health around the world. Recent studies have indicated that long non-coding RNAs (lncRNAs) occupy an important position in various human cancers. However, the function and mechanism of lncRNA DNMBP antisense RNA 1 (DNMBP-AS1) in PCa is rarely investigated. RT-qPCR analysis was used to test gene expression. CCK-8, colony formation, EdU
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Trophectoderm non-coding RNAs reflect the higher metabolic and more invasive properties of young maternal age blastocysts Syst. Biol. Reprod. Med. (IF 2.1) Pub Date : 2022-12-28 Panagiotis Ntostis, Grace Swanson, Georgia Kokkali, David Iles, John Huntriss, Agni Pantou, Maria Tzetis, Konstantinos Pantos, Helen M. Picton, Stephen A. Krawetz, David Miller
Abstract Increasing female age is accompanied by a corresponding fall in her fertility. This decline is influenced by a variety of factors over an individual’s life course including background genetics, local environment and diet. Studying both coding and non-coding RNAs of the embryo could aid our understanding of the causes and/or effects of the physiological processes accompanying the decline including
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Mitochondrial DNA copy number in cumulus granulosa cells as a predictor for embryo morphokinetics and chromosome status Syst. Biol. Reprod. Med. (IF 2.1) Pub Date : 2022-11-25 Pitra Rahmawati, Budi Wiweko, Arief Boediono
Abstract While morphokinetic evaluation of embryos has become the most commonly used technique in IVF to select embryos for transfer, studies have demonstrated that mitochondrial DNA (mtDNA) copy number is correlated with embryo viability and transfer outcomes. Correspondingly, this cohort study aims to evaluate the association between the mtDNA copy number in cumulus granulosa cells (CGCs) with embryo