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Reference gene considerations for toxicological assessment of the flame retardant triphenyl phosphate in an in vitro fish embryonic model J. Appl. Toxicol. (IF 2.7) Pub Date : 2024-09-19 Logan Germain, Delaine Pereira, Louise M. Winn
The reliability of relative quantification RT‐qPCR depends upon the gene of interest being normalized to one or more reference genes, with the assumption that the chosen reference genes do not experience altered expression with experimental conditions. The correct choice of stable reference genes is critical when investigating alterations to gene transcript levels following exposure to endocrine and
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Nebivolol hydrochloride and its impurities induce pseudo‐allergic reactions via mast cell activation J. Appl. Toxicol. (IF 2.7) Pub Date : 2024-09-17 Liju Yu, Yi Shan, Jiayu Lu, Huaizhen He
Nebivolol hydrochloride is a third‐generation β‐blocker commonly used to treat cardiovascular diseases. However, it has been reported to induce allergic reactions in clinical use which deserves much attention. Therefore, this study focused on the ability of two isomers of nebivolol and chiral isomer impurities to induce allergic reactions. Our findings demonstrate that both nebivolol and two isomeric
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Nuciferine protects hyperandrogen‐injured ovarian granulosa cells by inhibiting ferroptosis via SOX2‐mediated activation of the SLC7A11/GPX4 axis J. Appl. Toxicol. (IF 2.7) Pub Date : 2024-09-17 Hongyu Yang, Shichao Chen, Shanshan Yin, Qi Ding
Polycystic ovary syndrome (PCOS) is a common endocrine disorder that can cause menstrual irregularities, infertility, polycystic ovaries, and metabolic abnormalities. Female reproductive health and quality of life are significantly affected by PCOS, which has recently been associated with ferroptosis in granulosa cells (GCs). Nuciferine (NF) is a naturally extracted substance with multiple pharmacological
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Flame retardant, hexabromocyclododecane, increases production of pro‐inflammatory cytokines, interleukin 1‐beta and interleukin 6, in human immune cells J. Appl. Toxicol. (IF 2.7) Pub Date : 2024-09-17 April Falconer‐Turner, Kameron Brooks, Eseoghene Ogaga, Margaret M. Whalen
Hexabromocyclododecane (HBCD) is an environmental contaminant due to its use as a flame retardant in a variety of applications ranging from building insulation, furniture upholstery, and housing for appliances and electronics. HBCD is found in wildlife, human breastmilk, and serum. Interleukin 1‐beta (IL‐1β) and interleukin 6 (IL‐6) are pro‐inflammatory cytokines, whose dysregulation is associated
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The ameliorative potential of metformin against aluminum‐induced neurotoxicity: Insights from in vitro studies J. Appl. Toxicol. (IF 2.7) Pub Date : 2024-09-14 Sonia Sanajou, Anil Yirün, Göksun Demirel, Pinar Erkekoğlu, Gönül Şahin, Terken Baydar
Alzheimer's disease (AD) is increasingly recognized as a metabolic disorder, often referred to as type 3 diabetes, due to its strong association with insulin resistance. Chronic exposure to aluminum, a known neurotoxin, has been identified as a significant risk factor in the development and progression of AD. This study explores the potential of metformin, a common anti‐diabetic drug, to mitigate aluminum‐induced
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Issue Information J. Appl. Toxicol. (IF 2.7) Pub Date : 2024-09-10
No abstract is available for this article.
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Bifidobacterium longum subsp. infantis YLGB‐1496—Toxicological evaluation J. Appl. Toxicol. (IF 2.7) Pub Date : 2024-09-10 Jian Zhao, Yueyi Guo, Qiuyue Jiang, Hanglian Lan, Wei‐Lian Hung, Barry Lynch
Bifidobacterium infantis YLGB‐1496, originally isolated from breast milk from a Taiwanese mother, is under study for use as a probiotic. As part of safety assessment, an Ames, in vivo mouse micronucleus, and in vivo mouse spermatocyte chromosome aberration assay were conducted along with a 13‐week oral rat toxicity study. B. infantis YLGB‐1496 had no activity in any of the genotoxicity assays. Administration
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Concentration‐dependent effects of the nerve agents cyclosarin and VX on cytochrome P450 in a HepaRG cell‐based liver model J. Appl. Toxicol. (IF 2.7) Pub Date : 2024-09-04 Gabriele Horn, Franziska Frielingsdorf, Tobias Demel, Simone Rothmiller, Franz Worek, Niko Amend
The exposure to highly toxic organophosphorus (OP) compounds, including pesticides and nerve agents, is an ongoing medical challenge. OP can induce the uncontrolled overstimulation of the cholinergic system through inhibition of the enzyme acetylcholinesterase (AChE). The cytochrome P450 (CYP) enzymes in the liver play a predominant role in the metabolism of xenobiotics and are involved in the oxidative
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Effect of vehicle on the in vitro penetration and metabolism of genistein and daidzein in ex vivo skin explants and the Phenion full‐thickness skin model J. Appl. Toxicol. (IF 2.7) Pub Date : 2024-08-28 Camille Géniès, Corinne Jeanjean, Abdulkarim Najjar, Andreas Schepky, Daniela Lange, Jochen Kühnl, Eric Fabian, Anne Zifle, Helene Duplan, Nicola J. Hewitt, Carine Jacques
In a read‐across assessment of the safety of genistein and daidzein in cosmetic products, additional information was required to account for differences in their systemic exposure after topical application in a typical body lotion formulation. Therefore, we measured the penetration and metabolism of two doses (3 and 30 nmoles/cm2) of genistein and daidzein applied in ethanol and in a body formulation
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Characterization of the in vitro penetration and first‐pass metabolism of genistein and daidzein using human and pig skin explants and Phenion full‐thickness skin models J. Appl. Toxicol. (IF 2.7) Pub Date : 2024-08-28 Camille Géniès, Corinne Jeanjean, Abdulkarim Najjar, Andreas Schepky, Daniela Lange, Jochen Kühnl, Eric Fabian, Anne Zifle, Hélène Duplan, NIcola J. Hewitt, Carine Jacques
OECD test guideline compliant skin penetration studies, which also comply with the SCCS basic criteria, are lacking for genistein and daidzein. Therefore, we have measured their penetration and metabolism using ex vivo explants of fresh (i.e., metabolically viable) pig skin, fresh and frozen human skin, and Phenion full‐thickness (FT) models. Preliminary studies using fresh pig skin helped to define
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Epigenetic alternations in the SYP and DLG4 genes due to low‐level methylmercury exposure during neuronal differentiation in vitro J. Appl. Toxicol. (IF 2.7) Pub Date : 2024-08-27 Hisaka Kurita, Haruka Masuda, Ayu Okuda, Suzuna Go, Kazuki Ohuchi, Hiroki Yoshioka, Masatake Fujimura, Isao Hozumi, Masatoshi Inden
Methylmercury (MeHg) is an environmental toxin known to damage the central nervous system. When pregnant women ingest seafood, which may contain accumulated MeHg, fetal development may be affected. The embryonic period, a time of major epigenetic change, is susceptible to epigenetic disruptions due to chemical exposure. Therefore, understanding the molecular mechanism underlying MeHg's effects on neuronal
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Appearance of sex‐determining region Y‐box 9 (SOX9)‐ and glutathione S‐transferase placental form (GST‐P)‐positive hepatocytes as possible carcinogenic events in the early stage of furan‐induced hepatocarcinogenesis J. Appl. Toxicol. (IF 2.7) Pub Date : 2024-08-22 Daisuke Hibi, Meili Soma, Yuta Suzuki, Shinji Takasu, Yuji Ishii, Takashi Umemura
Furan, the basic skeleton of various flavoring agents, induces cholangiocellular tumors with higher incidences in the caudate lobe and hepatocellular tumors without the lobe specificity in rats, but the mechanism is unclear. We investigated the lobe distribution of possible carcinogenic events. Furan caused proliferation/infiltration of oval and inflammatory cells prominently in the caudate lobe as
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Bacillus subtilis ZB423: 90‐Day repeat dose oral (gavage) toxicity study in Wistar rats J. Appl. Toxicol. (IF 2.7) Pub Date : 2024-08-22 Vijayakumar K. N., Swati S. Bamne, Johnson Crasta, Satish Kumar C., Ramakrishna M. Bhat, Bentley M. Shuster, John W. K. Oliver, Zachary D. Abbott
The novel genetically modified probiotic Bacillus subtilis ZB423 was assessed in a 90‐day repeated‐dose oral toxicity study adhering to Good Laboratory Practice (GLP) and Organization for Economic Cooperation and Development (OECD) guidelines. Spray‐dried spores at a concentration of 1.1E12 CFU/g were administered at doses of 130, 260, and 519 mg/kg body weight/day correlating to 1.43 × 1011, 2.86
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Toxicity of soil leaching liquor from coking plant in developmental zebrafish embryos/larvae model J. Appl. Toxicol. (IF 2.7) Pub Date : 2024-08-21 Guangchao Yang, Jining Liu, Qian Yang, Wen Gu
The coking industry in China is the largest coke supplier in the world. Contaminated soil in industrial areas poses a serious threat to human and ecosystems. Most of the studies investigated the toxicity of soil from coking plant on soil microorganisms, while the toxic effects of soil leaching liquor on aquatics are limited. In this study, the composition of soil leaching liquor from a coking plant
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Composition of e‐cigarette aerosols: A review and risk assessment of selected compounds J. Appl. Toxicol. (IF 2.7) Pub Date : 2024-08-16 Jonathan Heywood, Grayson Abele, Blake Langenbach, Sydney Litvin, Sarah Smallets, Dennis Paustenbach
The potential harms and benefits of e‐cigarettes, or electronic nicotine delivery systems (ENDS), have received significant attention from public health and regulatory communities. Such products may provide a reduced risk means of nicotine delivery for combustible cigarette smokers while being inappropriately appealing to nicotine naive youth. Numerous authors have examined the chemical complexity
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Issue Information J. Appl. Toxicol. (IF 2.7) Pub Date : 2024-08-15
No abstract is available for this article.
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Esculin alleviates lipopolysaccharide (LPS)–induced pneumonia by regulating the USP7/MAPK14 axis J. Appl. Toxicol. (IF 2.7) Pub Date : 2024-08-15 Lijuan Wang, Na Li, Yanan Wang, Xu Chen
Pneumonia is a serious and life‐threatening lung inflammation with high morbidity and mortality. Accumulating evidence has suggested that esculin, a derivative of coumarin, possesses potent anti‐inflammatory effects. This study is designed to explore the pharma role and underlying mechanism of esculin against lipopolysaccharides (LPS)‐induced pneumonia. TC‐1 cells were stimulated by LPS to mimic the
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Physiologically‐based pharmacokinetic model of in vitro porcine ear skin permeation for drug delivery research J. Appl. Toxicol. (IF 2.7) Pub Date : 2024-08-13 Laura Krumpholz, Sebastian Polak, Barbara Wiśniowska
In silico techniques, such as physiologically based pharmacokinetic modeling (PBKP), are recently gaining importance. Computational methods in drug discovery and development and the generic drugs industry enhance research effectiveness by saving time and money and avoiding ethical issues. One key advantage is the ability to conduct toxicology studies without risking harm to living beings. This study
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Taurine and enzymatically modified isoquercitrin protected against methotrexate‐induced deteriorations in the conductivity and rhythmicity of the heart in rats: Antioxidant, anti‐inflammatory, and histological architecture approach J. Appl. Toxicol. (IF 2.7) Pub Date : 2024-08-13 Marwa M. Mahmoud, Seham A. El‐Batran, Rehab Hegazy, Wael M. El‐Sayed
Cardiotoxicity is one of the most devastating complications of cancer treatment by methotrexate (MTX). The present study aimed to investigate the potential anti‐cardiotoxic efficacy of taurine (Tau) and enzymatically modified isoquercitrin (EMIQ) alone or combined against MTX‐induced cardiotoxicity in adult male rats. A total of 36 rats were randomly divided into six groups (six animals each): control
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Non‐chemical stresses do not strongly induce male offspring in Daphnia magna ascertained using the short‐term juvenile hormone activity screening assay J. Appl. Toxicol. (IF 2.7) Pub Date : 2024-08-13 Haruna Watanabe, Ryoko Abe, Norihisa Tatarazako, Hiroshi Yamamoto
Juvenile hormone (JH), together with ecdysone, regulates molting, metamorphosis, growth, and reproduction in arthropods. The effects of its analogs used as insecticides on nontarget species are of concern. Since JH and JH analogs (JHAs) induce male offspring in daphnids, which generally reproduce by parthenogenesis, short‐term JH activity screening assay (JHASA) using the male offspring ratio as an
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In silico analysis of endocrine‐disrupting potential of triclosan, bisphenol A, and their analogs and derivatives J. Appl. Toxicol. (IF 2.7) Pub Date : 2024-08-12 Larisa Đurić, Maja Milanović, Jovana Drljača Lero, Nataša Milošević, Nataša Milić
Owning to the increasing body of evidence about the ubiquitous exposure to endocrine disruptors (EDCs), particularly bisphenol A (BPA), and associated health effects, BPA has been gradually substituted with insufficiently tested structural analogs. The unmanaged excessive use of antimicrobial agents such as triclosan (TCS) during the COVID‐19 outbreak has also raised concerns about its possible interferences
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Risk assessment of 2β,3β‐19α‐trihydroxyursolic acid from Rubus imperialis (Rosaceae) in HepG2/C3A cells via genotoxicity, metabolism, and cell growth J. Appl. Toxicol. (IF 2.7) Pub Date : 2024-08-12 Bruna Oshiiwa, Aline Pereira da Silva, Greice Rafaele Alves, Valdir Cechinel Filho, Rivaldo Niero, Isabel O'Neill de Mascarenhas Gaivão, Liana Martins de Oliveira, Luan Vitor Alves de Lima, Mário Sérgio Mantovani, Edson Luis Maistro
Rubus imperialis (Rosaceae) is a Brazilian medicinal plant that already exhibited therapeutical perspectives. However, previous studies revealed cellular and/or genetic toxicity of extracts from aerial parts of this plant, as well as other species of the Rubus genus. Being 2β,3β‐19α‐trihydroxyursolic acid (2B) one of the major compounds of this plant, with proven pharmacological effect, it is important
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90‐day oral toxicity study in rats of a protein‐rich powder derived from Xanthobacter sp. SoF1 J. Appl. Toxicol. (IF 2.7) Pub Date : 2024-08-05 Bean Choi, Róbert Glávits, Timothy S. Murbach, John R. Endres, Gábor Hirka, Ilona Pasics Szakonyiné
Xanthobacter sp. SoF1 (SoF1) is an autotrophic hydrogen‐oxidizing bacteria that produces protein‐rich biomass and has potential to be an alternative protein source that is more environmentally sustainable than animal and plant derived proteins. A protein‐rich powder derived from SoF1 was the test material in a 90‐day repeated‐dose oral toxicity study to explore major toxic effects, demonstrate target
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The dimer effect: A refinement approach towards skin sensitization assessment in‐chemico using Amino acid Derivative Reactivity Assay J. Appl. Toxicol. (IF 2.7) Pub Date : 2024-08-03 Ratnadeep Paul Choudhury, Akanksha Singh, Eldho Mathai, DGS Sudhakar, Fleur Tourneix, Nathalie Alépée, Francoise Gautier
Skin sensitization is a key endpoint for safety assessment, especially for cosmetics and personal care products. The adverse outcome pathway for skin sensitization and the chemical and biological events driving the induction of human skin sensitization are now well understood. Several non‐animal test methods have been developed to predict sensitizer potential by measuring the impact of chemical sensitizers
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Melatonin intervention to prevent nanomaterial exposure‐induced damages: A systematic review and meta‐analysis of in vitro and in vivo studies J. Appl. Toxicol. (IF 2.7) Pub Date : 2024-08-02 Xuejiao Wang, Yang Zhou, Dongli Xie, Fei Yin, Yunxia Liang, Xiaogang Luo
Given its antioxidant, anti‐inflammatory, and antiapoptotic properties, melatonin (MEL), a health‐caring food to improve sleep disorders, is hypothesized to protect against nanomaterial exposure‐induced toxicity. However, the conclusion derived from different studies seemed inconsistent. A meta‐analysis of all available preclinical studies was performed to examine the effects of MEL on nanomaterial‐induced
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Influences of TiO2 nanoparticle and fipronil co‐exposure on metabolite profiles in mouse intestines J. Appl. Toxicol. (IF 2.7) Pub Date : 2024-07-30 Canyang Wang, Zhengzheng Zhou, Yayu He, Juan Li, Yi Cao
Food contaminates, such as insecticide, may influence the toxicity of nanoparticles (NPs) to intestine. The present study investigated the combined toxicity of TiO2 NPs and fipronil to male mouse intestine. Juvenile mice (8 weeks) were orally exposed to 5.74 mg/kg TiO2 NPs, 2.5 mg/kg fipronil, or both, once a day, for 5 days. We found that both TiO2 NPs and fipronil induced some pathological changes
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Endocrine disruption of adipose physiology: Screening in SGBS cells J. Appl. Toxicol. (IF 2.7) Pub Date : 2024-07-24 Jan Kucera, Zuzana Chalupova, Martin Wabitsch, Julie Bienertova‐Vasku
The increasing use of industrial chemicals has raised concerns regarding exposure to endocrine‐disrupting chemicals (EDCs), which interfere with developmental, reproductive and metabolic processes. Of particular concern is their interaction with adipose tissue, a vital component of the endocrine system regulating metabolic and hormonal functions. The SGBS (Simpson Golabi Behmel Syndrome) cell line
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Adverse effects of cadmium on lymphoid organs, immune cells, and immunological responses J. Appl. Toxicol. (IF 2.7) Pub Date : 2024-07-24 Priyanka Kumari, Kumari Pragati Nanda, Hena Firdaus
Humans and animals possess robust immune systems to safeguard against foreign pathogens. However, recent reports suggest a greater incidence of immunity breakdown due to exposure to environmental pollutants, with heavy metals emerging as potential candidates in such immuno‐toxicological studies. While we have extensive data on the general toxicity resulting from exposure to heavy metals, comprehensive
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Hepatic enzyme induction and its potential effect on thyroid hormone metabolism in the metamorphosing tadpole of Xenopus laevis (African clawed frog) J. Appl. Toxicol. (IF 2.7) Pub Date : 2024-07-23 Kohei Wada, Takafumi Yamaguchi, Hitoshi Tanaka, Takuo Fujisawa
Hepatic enzyme induction, an inherent defense system against xenobiotics, is known to simultaneously affect endocrine system functions in mammals under specific conditions, particularly thyroid hormone (TH) regulation. While this phenomenon has been studied extensively, the pathway leading to this indirect thyroid effect in mammals has unclear applicability to amphibians, despite the importance of
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Isoflurane preconditioning attenuates OGD/R‐induced cardiomyocyte cytotoxicity by regulating the miR‐210/BNIP3 axis J. Appl. Toxicol. (IF 2.7) Pub Date : 2024-07-20 Dongbo Zhang, Qiaoling Wu, Feifei Liu, Tu Shen, Siqi Dai
Isoflurane, a commonly used inhaled anesthetic, has been found to have a cardioprotective effect. However, the precise mechanisms have not been fully elucidated. Here, we found that isoflurane preconditioning enhanced OGD/R‐induced upregulation of miR‐210, a hypoxia‐responsive miRNA, in AC16 human myocardial cells. To further test the roles of miR‐210 in regulating the effects of isoflurane preconditioning
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Taurocholic acid represents an earlier and more sensitive biomarker and promotes cholestatic hepatotoxicity in ANIT‐treated rats J. Appl. Toxicol. (IF 2.7) Pub Date : 2024-07-19 Hang Yang, Tingting Yang, Jiaxin Ding, Xue Wang, Xi Chen, Jia Liu, Ting Shu, Ziteng Wu, Lixin Sun, Xin Huang, Zhenzhou Jiang, Luyong Zhang
Bile acid homeostasis is crucial for the normal physiological functioning of the liver. Disruptions in bile acid profiles are closely linked to the occurrence of cholestatic liver injury. As part of our diagnostic and therapeutic approach, we aimed to investigate the disturbance in bile acid profiles during cholestasis and its correlation with cholestatic liver injury. Before the occurrence of liver
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Ferroptosis: Latest evidence and perspectives on plant‐derived natural active compounds mitigating doxorubicin‐induced cardiotoxicity J. Appl. Toxicol. (IF 2.7) Pub Date : 2024-07-19 Boyu Wang, Jiameng Wang, Changxing Liu, Chengjia Li, Tianwei Meng, Jia Chen, Qingnan Liu, Wang He, Zhiping Liu, Yabin Zhou
Doxorubicin (DOX) is a chemotherapy drug widely used in clinical settings, acting as a first‐line treatment for various malignant tumors. However, its use is greatly limited by the cardiotoxicity it induces, including doxorubicin‐induced cardiomyopathy (DIC). The mechanisms behind DIC are not fully understood, but its potential biological mechanisms are thought to include oxidative stress, inflammation
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Oleanolic acid induces hepatic injury by disrupting hepatocyte tight junction and dysregulation of farnesoid X receptor‐mediated bile acid efflux transporters J. Appl. Toxicol. (IF 2.7) Pub Date : 2024-07-19 Li Zeng, Jianxiang Huang, Yi Wang, Yan Hu, Shaoyu Zhou, Yuanfu Lu
Oleanolic acid (OA) is a naturally occurring pentacyclic triterpene compound that has been reported to cause cholestatic liver injury. However, the regulation and pathogenic role of bile acids in OA‐induced development of cholestatic liver injury remains largely unclear. Farnesoid X receptor (FXR) is a metabolic nuclear receptor that plays an important role in bile acid homeostasis in the liver by
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Piceatannol induces caspase‐dependent apoptosis by modulating intracellular reactive oxygen species/mitochondrial membrane potential and enhances autophagy in neuroblastoma cells J. Appl. Toxicol. (IF 2.7) Pub Date : 2024-07-15 Ebru Güçlü, İlknur Çınar Ayan, Sümeyra Çetinkaya, Hatice Gül Dursun, Hasibe Vural
The aim of this study was to evaluate the anticancer effects of piceatannol, a natural stilbenoid, on human neuroblastoma cells. In order to accomplish this goal, we performed various cellular assays, including the XTT cell proliferation assay for cell viability, colony formation assay for colony formation capacity, FITC Annexin V and cell death detection kit for apoptosis, matrigel invasion assay
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Featured Cover J. Appl. Toxicol. (IF 2.7) Pub Date : 2024-07-15 Yitong Liu, Michael Lawless, Miao Li, Kiara Fairman, Michelle R. Embry, Constance A. Mitchell
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Issue Information J. Appl. Toxicol. (IF 2.7) Pub Date : 2024-07-15
No abstract is available for this article.
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Berberine prevents against myocardial injury induced by acute β‐adrenergic overactivation in rats J. Appl. Toxicol. (IF 2.7) Pub Date : 2024-07-10 Yalin Yang, Shuang Jiang, Yu Mu, Chilu Liu, Yanxing Han, Jiandong Jiang, Yuhong Wang
The overactivation of β‐adrenergic receptors (β‐ARs) can result in acute myocardial ischemic injury, culminating in myocardial necrosis. Berberine (BBR) has exhibited promising potential for prevention and treatment in various heart diseases. However, its specific role in mitigating myocardial injury induced by acute β‐AR overactivation remains unexplored. This study aimed to investigate the effects
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Exploring developmental MeHg impact on extraembryonic and cardiac vessels and its effect on cardiomyocyte contractility J. Appl. Toxicol. (IF 2.7) Pub Date : 2024-07-09 Nathália Ronconi‐Krüger, Yara Maria Rauh Müller, Evelise Maria Nazari
The toxicity of methylmercury (MeHg) during embryonic development is a relevant issue that remains unclear and deserves investigation. In this sense, there is evidence that links the intake of contaminated food with cardiovascular pathologies in human adults and children. Thus, this study aimed to verify the impact of MeHg on the structure and integrity of extraembryonic and cardiac blood vessels and
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Nonclinical safety and immunogenicity assessment of a combined DTacP vaccine in animal models J. Appl. Toxicol. (IF 2.7) Pub Date : 2024-07-09 Shihui Li, Hui Fu, Shouzhi Yu, Yuxiu Zhao, Ting Liu, Ling Wang, Na Zhang, Wei Wang, Baifeng Yang, Peng He, Yancen Guo, Shaoting Qiu, Yuntao Zhang
The (diphtheria, tetanus, and pertussis [acellular, component] [DTacP]) vaccine is a combined vaccine designed to prevent three potentially fatal diseases including pertussis, tetanus, and diphtheria in both children and adults. We utilized advanced technology to develop a novel DTacP vaccine that was previously unavailable in China. The nonclinical studies were performed to evaluate the immunogenicity
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Is micronucleus assay a useful marker in gingiva, tongue, and palate for evaluating cytogenetic damage induced by chemical, physical, and biological agents in vivo? A systematic review with meta‐analysis J. Appl. Toxicol. (IF 2.7) Pub Date : 2024-07-02 Thiago Guedes Pinto, Wilton Mitsunari Takeshita, Ana Claudia Muniz Renno, Patricia Ramos Cury, Jean Junes dos Santos, Daniel Araki Ribeiro
The present systematic review (SR) aims to evaluate manuscripts in order to help further elucidate the following question: is the micronucleus assay (MA) also a useful marker in gingiva, tongue, and palate for evaluating cytogenetic damage in vivo? A search was performed through the electronic databases PubMed/Medline, Scopus, and Web of Science, all studies published up to December 2023. The comparisons
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An evaluation of the genotoxicity and 90‐day repeated dose oral toxicity in rats of Porphyridium purpureum J. Appl. Toxicol. (IF 2.7) Pub Date : 2024-07-02 Timothy S. Murbach, Róbert Glávits, John R. Endres, Gábor Hirka, Adél Vértesi, Erzsébet Béres, Ilona Pasics Szakonyiné
Interest in microalgae products for use in food is increasing, as demands for sustainable and cost‐effective food choices grow due to the escalating global population and increase in climate‐related struggles with agriculture. Toxicological assessments of some species of microalgae have been conducted, but there were little data available for the oral consumption of the red microalgae Porphyridium
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Possible pathogenic mechanisms for doxorubicin‐induced splenic atrophy in a human breast cancer xenograft mouse model J. Appl. Toxicol. (IF 2.7) Pub Date : 2024-06-29 Jianjie Xue, Bing Ye, Mengqi Sun
Doxorubicin‐based chemotherapy is a widely used first‐line treatment for breast cancer, yet it is associated with various side effects, including splenic atrophy. However, the pathogenic mechanisms underlying doxorubicin‐induced atrophy of the spleen remain unclear. This study investigates that doxorubicin treatment leads to splenic atrophy through several interconnected pathways involving histological
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Impact of oxidative stress induced by heavy metals on ovarian function J. Appl. Toxicol. (IF 2.7) Pub Date : 2024-06-28 Chengqi Xiao, Dongmei Lai
As a crucial organ of the female reproductive system, the ovary has both reproductive and endocrine functions. Oxidative stress refers to an increase in intracellular reactive oxygen species (ROS), which play a role in the normal physiological activity of the ovary. However, excessive ROS can cause damage to the ovary. With the advancement of human industrial activities, heavy metal pollution has become
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Examining the hepatotoxic potential of cannabidiol, cannabidiol‐containing hemp extract, and cannabinol at consumer‐relevant exposure concentrations in primary human hepatocytes J. Appl. Toxicol. (IF 2.7) Pub Date : 2024-06-26 Anneliese Striz, Yang Zhao, Estatira Sepehr, Cory Vaught, Kirsten Eckstrum, Kyra Headrick, Jeffrey Yourick, Robert Sprando
Hemp extracts and consumer products containing cannabidiol (CBD) and/or other phytocannabinoids derived from hemp have entered the marketplace in recent years. CBD is an approved drug in the United States for the treatment of certain seizure disorders. While effects of CBD in the liver have been well characterized, data on the effects of other cannabinoids and hemp extracts in the liver and methods
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Pancreatic β cell models for screening insulin secretagogues and cytotoxicity J. Appl. Toxicol. (IF 2.7) Pub Date : 2024-06-22 Pranjali Potdar, Avinash Kharat, Avinash Sanap, Supriya Kheur, Ramesh Bhonde
In the past 2–3 decades, numerous attempts have been made to create an insulin‐secreting β cell line that maintains normal insulin secretion. However, primary β cell cultures have finite life and, therefore, cannot be used for long‐term experiments. The most widely used insulin‐secreting cell lines are Insulinoma‐1, rat insulinoma cell line, hamster pancreatic β cell line, mouse insulinoma, and β tumor
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Potential consequences of nitric oxide release: An improved model informing worker safety J. Appl. Toxicol. (IF 2.7) Pub Date : 2024-06-21 Gautham Venugopalan, Rocco Casagrande, Noah Gunther, Rashmi Prasad, Shawn Jackson
Both nitric oxide (NO) and nitrogen dioxide (NO2) gasses are toxic to humans but are commonly found in industrial settings such as semiconductor manufacturing sites. Due to the spontaneous oxidation of NO to NO2 under ambient conditions, individuals working with NO may in fact be exposed to both gasses in the case of an accidental release. Unfortunately, most safety materials provided to NO users do
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Issue Information J. Appl. Toxicol. (IF 2.7) Pub Date : 2024-06-21
No abstract is available for this article.
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Exposure to thiourea during the early stages of development impedes the formation of the swim bladder in zebrafish larvae. J. Appl. Toxicol. (IF 2.7) Pub Date : 2024-06-18 Lakshmi Pillai,Shantanu Karandikar,Kamya Pandya,Aishwarya V M,Anjali Singh,Suresh Balakrishnan
Thiourea, a widely used agrochemical, is known to inhibit the activity of thyroid peroxidase, a key enzyme in the biosynthetic pathway of thyroid hormones. Thyroid insufficiency compromises the basal metabolic rate in warm-blooded organisms and embryonic development in vertebrates. In this study, we looked for developmental defects by exposing the zebrafish embryos to an environmentally relevant dose
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Oral subchronic toxicity study and genetic toxicity evaluation of mitoquinone mesylate. J. Appl. Toxicol. (IF 2.7) Pub Date : 2024-06-11 E Siobhan Mitchell,Shawna Lemke,Brendon Woodhead,David Coleman
Mitochondrial dysfunction and excessive reactive oxygen species production contributes to the pathophysiology of aging. Coenzyme Q10 is thought to protect mitochondria from oxidative damage; thus, mitoquinone was developed as mitochondria-targeted analogue with similar antioxidant activity. Mitoquinone is the oxidized form of mitoquinol. Mitoquinone/mitoquinol mesylate has been proposed as a food ingredient
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Is genotoxicity a suitable biomarker for monitoring anabolic-androgenic steroids exposure in vivo? A systematic review and meta-analysis. J. Appl. Toxicol. (IF 2.7) Pub Date : 2024-06-05 Thiago Guedes Pinto,Ingra Tais Malacarne,Wilton Mitsunari Takeshita,Milena de Barros Viana,Ana Claudia Muniz Renno,Daniel Araki Ribeiro
Steroids stand for a class of hormones (natural and synthetic) known to be helpful for a number of disorders. Despite the aforementioned beneficial effects of using these hormones, anabolic-androgenic steroids (AAS) are also widely abused in a non-therapeutic manner for muscle-building and strength-increasing properties that may lead to genotoxicity in different tissues. The present study aims to understand
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Differential impacts of nonsteroidal anti-inflammatory drugs on lifespan and healthspan in aged Caenorhabditis elegans. J. Appl. Toxicol. (IF 2.7) Pub Date : 2024-06-05 Jia-Jun Tu,Zhen-Zhen Yu,Mei-Ling Ou,Jin-Xiong Cen,Kun Xue,Jing Zhou,Shao-Jun Li,Guo-Dong Lu
Aging and age-related diseases are intricately associated with oxidative stress and inflammation. Nonsteroidal anti-inflammatory drugs (NSAIDs) have shown their promise in mitigating age-related conditions and potentially extending lifespan in various model organisms. However, the efficacy of NSAIDs in older individuals may be influenced by age-related changes in drug metabolism and tolerance, which
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Research advance of occupational exposure risks and toxic effects of semiconductor nanomaterials. J. Appl. Toxicol. (IF 2.7) Pub Date : 2024-06-04 Jiawei Wu,Xiaomeng Ding,Yanting Pang,Qing Liu,Jialin Lei,Haopeng Zhang,Ting Zhang
In recent years, semiconductor nanomaterials, as one of the most promising and applied classes of engineered nanomaterials, have been widely used in industries such as photovoltaics, electronic devices, and biomedicine. However, occupational exposure is unavoidable during the production, use, and disposal stages of products containing these materials, thus posing potential health risks to workers.
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The aryl hydrocarbon receptor pathway is a marker of lung cell activation but does not play a central pathologic role in engineered stone-associated silicosis. J. Appl. Toxicol. (IF 2.7) Pub Date : 2024-06-04 Yong Song,Seiha Yen,Katherine Southam,Sharyn Gaskin,Ryan F Hoy,Graeme R Zosky
Engineered stone-associated silicosis is characterised by a rapid progression of fibrosis linked to a shorter duration of exposure. To date, there is lack of information about molecular pathways that regulates disease development and the aggressiveness of this form of silicosis. Therefore, we compared transcriptome responses to different engineered stone samples and standard silica. We then identified
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A 90-day preclinical toxicological evaluation in rats of a highly purified and concentrated mulberry leaf extract. J. Appl. Toxicol. (IF 2.7) Pub Date : 2024-06-04 Timothy S Murbach,Róbert Glávits,John R Endres,Gábor Hirka,Ilona Pasics Szakonyiné
Mulberry (genus Morus) leaves have long been used as a human food, especially in Asia, and animal feed. More recently, mulberry leaf extracts have been introduced as a convenient way to consume mulberry for non-nutritional functional effects. Reducose® 5% is an Morus alba leaf extract that has been highly purified and standardized to a content of 5 ± 0.5% 1-deoxynojirimycin, a naturally present polyhydroxylated
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Evaluation of the potential role of glutamatergic, cholinergic, and nitrergic systems in the dopamine release induced by the pesticide glyphosate in rat striatum. J. Appl. Toxicol. (IF 2.7) Pub Date : 2024-06-03 Carmen Costas-Ferreira,Rafael Durán,Lilian R F Faro
Glyphosate (GLY) is a pesticide that severely alters nigrostriatal dopaminergic neurotransmission, inducing great increases in dopamine release from rat dorsal striatum. This GLY-induced striatal dopamine overflow occurs through mechanisms not yet fully understood, hence the interest in evaluating the role of other neurotransmitter systems in such effects. So, the main objective of this mechanistic
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Rsad2 mediates Bisphenol A-induced actin cytoskeletal disruption in mouse spermatocytes. J. Appl. Toxicol. (IF 2.7) Pub Date : 2024-06-03 Xiao Jiang,Shengqi Sun,Chaofeng Shi,Kangle Liu,Yurui Yang,Jia Cao,Jing Gu,Jinyi Liu
Bisphenol A (BPA) is widely exposed in populations worldwide and has negative effects on spermatogenesis both in animals and humans. The homeostasis of the actin cytoskeleton in the spermatogenic epithelium is crucial for spermatogenesis. Actin cytoskeleton destruction in the seminiferous epithelium is one of the important reasons for BPA-induced spermatogenesis disorder. However, the underlying molecular
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Investigation of the mechanisms behind ochratoxin A‐induced cytotoxicity in human astrocytes and the protective effects of N‐acetylcysteine J. Appl. Toxicol. (IF 2.7) Pub Date : 2024-05-30 Che‐Sheng Chu, Ying‐Tso Chen, Wei‐Zhe Liang
Ochratoxin A (OTA) is a type of mycotoxin commonly found in raw and processed foods. It is essential to be aware of this toxin, as it can harm your health if consumed in high quantities. OTA can induce toxic effects in various cell models. However, a more comprehensive understanding of the harmful effects of OTA on human astrocytes is required. This study evaluated OTA's neurotoxic effects on the Gibco®
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Second‐generation antipsychotic quetiapine blocks voltage‐dependent potassium channels in coronary arterial smooth muscle cells J. Appl. Toxicol. (IF 2.7) Pub Date : 2024-05-27 Wenwen Zhuang, Seo‐Yeong Mun, Minju Park, Junsu Jeong, Hye Ryung Kim, Hongzoo Park, Eun‐Taek Han, Jin‐Hee Han, Wanjoo Chun, Hongliang Li, Won Sun Park
Voltage‐dependent K+ (Kv) channels play an important role in restoring the membrane potential to its resting state, thereby maintaining vascular tone. In this study, native smooth muscle cells from rabbit coronary arteries were used to investigate the inhibitory effect of quetiapine, an atypical antipsychotic agent, on Kv channels. Quetiapine showed a concentration‐dependent inhibition of Kv channels
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Biodistribution and preclinical safety profile of legubicin: A novel conjugate of doxorubicin and a legumain-cleavable peptide linker. J. Appl. Toxicol. (IF 2.7) Pub Date : 2024-05-23 Yan Wang,Liping Wei,Yuan Liu,Cheng Liu,Minbo Hou,Lu Zhou,Le Wang,Hua Li,Yunliang Qiu,JingMa
Legubicin is a novel conjugate of doxorubicin and a legumain-cleavable peptide linker. It has been developed to ameliorate the side effects of doxorubicin. Biodistribution in tumor-bearing mice, acute tolerance, and potential systemic toxic effects in Sprague-Dawley rats and beagle dogs of legubicin were assessed. Legubicin exists mainly as a protein complex in plasma after entering the circulation
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mtDNA amplifies beryllium sulfate‐induced inflammatory responses via the cGAS‐STING pathway in 16HBE cells J. Appl. Toxicol. (IF 2.7) Pub Date : 2024-05-23 Xiaodong Liu, Tianyi Jiang, Huiyun Jin, Chenxi Yan, Yuqi Tong, Jiaquan Ding, Yaqi Li, Lian Huang, Zhaohui Zhang
Beryllium sulfate (BeSO4) can cause inflammation through the mechanism, which has not been elucidated. Mitochondrial DNA (mtDNA) is a key contributor of inflammation. With mitochondrial damage, released mtDNA can bind to specific receptors (e.g., cGAS) and then activate related pathway to promote inflammatory responses. To investigate the mechanism of mtDNA in BeSO4‐induced inflammatory response in