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In vitro modelling of Parkinson's disease using 6-OHDA is associated with increased NQO2 activity Toxicol. In Vitro (IF 2.6) Pub Date : 2024-09-11 Ekaterina R. Verbovaya, Ilya A. Kadnikov, Ilya O. Logvinov, Tatyana A. Antipova, Mikhail V. Voronin, Sergei B. Seredenin
The pathogenesis of Parkinson's disease (PD) involves abnormalities in the metabolism of catecholamines. The enzyme quinone reductase 2 (NQO2) reduces quinone derivatives of catecholamines, which promotes the formation of reactive oxygen species (ROS), suggesting a role for NQO2 in the development of cellular damage typical of PD. In the present study, we investigated the relationship between 6-hydroxydophamine
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Chlorpyrifos-oxon induced neuronal cell death via endoplasmic reticulum stress-triggered apoptosis pathways Toxicol. In Vitro (IF 2.6) Pub Date : 2024-09-07 Baihuan Feng, Jingchun Lu, Wei Jiang, Nani Xu, Wenjun Sun
Chlorpyrifos (CPF) is one of the organophosphorus pesticides widely used throughout the world. Epidemiological studies suggested a link between CPF exposure and neurologic disorders, while the molecular mechanisms remain inconclusive. In the present study, we investigated the impacts of chlorpyrifos-oxon (CPO), the major toxic CPF metabolite, on cell apoptosis, and explored possible mechanism associated
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Applying cell painting in non-tumorigenic breast cells to understand impacts of common chemical exposures Toxicol. In Vitro (IF 2.6) Pub Date : 2024-09-06 Anagha Tapaswi, Nicholas Cemalovic, Katelyn M. Polemi, Jonathan Z. Sexton, Justin A. Colacino
The general population is exposed to many chemicals which have putative, but incompletely understood, links to breast cancer. Cell Painting is a high-content imaging-based in vitro assay that allows for unbiased measurements of concentration-dependent effects of chemical exposures on cellular morphology. We used Cell Painting to measure effects of 16 human exposure relevant chemicals, along with 21
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Effects of polystyrene micro- and nanoplastics on androgen- and estrogen receptor activity and steroidogenesis in vitro Toxicol. In Vitro (IF 2.6) Pub Date : 2024-09-05 Jeske van Boxel, Rani R.J. Khargi, Sandra M. Nijmeijer, Manuel T. Heinzelmann, Daniel Da Costa Pereira, Marja H. Lamoree, Majorie B.M. van Duursen
While many plastic additives show endocrine disrupting properties, this has not been studied for micro- and nanoplastics (MNPs) particles despite their ubiquitous presence in humans. The objective of this study was to determine the effects of various sizes and concentrations of polystyrene (PS)-MNPs (50–10,000 nm, 0.01–100 μg/mL) on estrogen- and androgen receptor (ER and AR) activity and steroidogenesis
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Risperidone-induced bioenergetic disruption in the isolated human peripheral blood monocytes Toxicol. In Vitro (IF 2.6) Pub Date : 2024-09-03 Bandar Alenazi, Huda A. Al Doghaither, Ayat B. Al-Ghafari, Ekramy M. Elmorsy
Risperidone (RIS) is a widely used antipsychotic drug with reported alteration in immune response. The current study investigated mitochondrial disruption as the underlying mechanism of RIS-induced immunotoxicity in isolated human peripheral blood monocytes (hPBM). RIS was cytotoxic to hPBM in exposure duration and concentration-dependent patterns. Functionally, RIS was shown to increase the release
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Alteration in folate carrier expression via histone deacetylase inhibition in BeWo human placental choriocarcinoma cells Toxicol. In Vitro (IF 2.6) Pub Date : 2024-09-03 Yuki Miyazawa, Ayako Furugen, Ryoichi Aoyagi, Haruna Kosugi, Ayako Nishimura, Takeshi Umazume, Katsuya Narumi, Masaki Kobayashi
Folates are essential nutrients for fetal development during pregnancy. Valproic acid (VPA), an inhibitor of histone deacetylases (HDACs), alters the expression of folate carriers in placental cells; however, the underlying mechanisms remain unclear. Here, we aimed to determine the profiles of folate carriers (folate receptor alpha [], solute carrier []-, and ) after inhibition of HDACs, especially
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Can TK-TD modelling bridge the gap between in vitro and in vivo mammalian toxicity data? Toxicol. In Vitro (IF 2.6) Pub Date : 2024-09-03 Thomas Martin, Mark E. Hodson, Helen Thompson, Victoria Hutter, Roman Ashauer
Repeated dietary dose testing is used to assess longer term toxicity of chemicals, such as pesticides, to mammals. However, the internal pesticide concentration varies significantly as feeding rate relative to body size fluctuates over time. Toxicokinetic-toxicodynamic (TK-TD) models can estimate internal toxicant concentration over time and link this directly to observed effects on endpoints such
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Effect of cannabidiol and hemp extract on viability and function of hepatocytes derived from human induced pluripotent stem cells Toxicol. In Vitro (IF 2.6) Pub Date : 2024-09-02 Kayla Campasino, Miranda R. Yourick, Yang Zhao, Estatira Sepehr, Cory Vaught, Jeffrey J. Yourick, Robert L. Sprando, Xiugong Gao
Since the passage of the 2018 Agriculture Improvement Act (2018 Farm Bill), the number of products containing cannabis-derived compounds available to consumers have rapidly increased. Potential effects on liver function as a result from consumption of products containing cannabidiol (CBD), including hemp extracts, have been observed but the mechanisms for the effects are not fully understood. In this
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Corrigendum to "Novel Diphenyl urea derivative serves as an inhibitor on human lung cancer cell migration by disrupting EMT via Wnt/β-catenin and PI3K/Akt signaling" [Toxicology in Vitro 69 (2020) 105000]. Toxicol. In Vitro (IF 2.6) Pub Date : 2024-09-02 Bingling Dai,Mengying Fan,Runze Yu,Qi Su,Bo Wang,Tianfeng Yang,Feng Liu,Yanmin Zhang
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Reliability and relevance of the ES®-RHE model for in vitro skin irritation test application Toxicol. In Vitro (IF 2.6) Pub Date : 2024-09-01 C.A. Mini, G. Ballestero, C.C. Munari, D.B. Carrão, B.C. Fonseca, N.C. Albuquerque, F. Marquele-Oliveira
methods have been widely used to assess adverse effects. Reconstructed Human Epidermis (RHE) poses as a fascinating test system employed to assess the dermal irritation hazard potential of chemicals. Although several RHE models are reported in the OECD Test Guideline No. 439, the OECD Document No. 220 encourages the scientific community to develop and validate new RHE test systems due to its relevance
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Assessment of ursolic acid effect on in vitro model of cardiac fibrosis Toxicol. In Vitro (IF 2.6) Pub Date : 2024-08-30 Samane Sadat Hosseiny, Zahra Esmaeili, Zeinab Neshati
This study aimed to evaluate the effects of ursolic acid (UA) on Angiotensin II (Ang II)-treated neonatal rat cardiac fibroblasts (rCFs) as an model of cardiac fibrosis. The rCFs were isolated from two-day-old neonatal rats. An model of cardiac fibrosis was established using 500 nm Ang II treatment for 48 h. The cells were then treated with 5 and 10 μM of UA for 24 and 48 h. Masson's trichrome staining
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Silencing Nrf2 in cisplatin resistant non-small cell lung cancer cells augments sensitivity towards EGFR inhibitor Toxicol. In Vitro (IF 2.6) Pub Date : 2024-08-22 Chandrani Fouzder, Alpana Mukhuty, Dipanjan Chattopadhyay, Snehasis Das, Sumit Kumar Hira, Rakesh Kundu
Recently, non-small cell lung cancer (NSCLC) has been the prime concern of cancer clinicians due to its high mortality rate worldwide. Cisplatin, a platinum derivative, has been used as a therapeutic option for treating metastatic NSCLC for several years. However, acquired, or intrinsic drug resistance to Cisplatin is the major obstacle to the successful treatment outcome of patients. Dysregulation
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Cytochrome P450-mediated metabolic interactions between donepezil and tadalafil in human liver microsomes Toxicol. In Vitro (IF 2.6) Pub Date : 2024-08-22 Jieun Yu, Ji Hyeon Ryu, Yong Ha Chi, Soo Heui Paik, Sang Kyum Kim
Donepezil and tadalafil, commonly prescribed among older persons to treat dementia and erectile dysfunction, respectively, are primarily metabolized by cytochrome P450 (CYP) 3A4. However, the drug-drug interactions (DDIs) of these drugs are unknown. Therefore, this study evaluated the CYP-mediated metabolic interaction between donepezil and tadalafil using pooled human liver microsomes (HLMs) to predict
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Unveiling the anticancer potential of Pestalotioprolide E, an unexplored macrolide: Targeting TRXR1-TRX1-ASK1-P38 signaling cascade in triple-negative breast cancer Toxicol. In Vitro (IF 2.6) Pub Date : 2024-08-22 Ruma Sarkar, Debobrata Paul, Akash Chatterjee, Anindita Bhattacharya, Sayantan Pradhan, Rajib Kumar Goswami, Prosenjit Sen
Triple-negative breast cancer (TNBC) is highly aggressive and metastatic in nature. Existing treatment modalities for TNBC are associated with severe side effects. Thioredoxin reductase (TRXR), the pivotal component of the thioredoxin system, remains overexpressed in various cancer cells including TNBC; promotes cell growth, proliferation, and metastasis, and inhibits apoptosis. Pestalotioprolide E
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Isobavachin induces autophagy-mediated cytotoxicity in AML12 cells via AMPK and PI3K/Akt/mTOR pathways Toxicol. In Vitro (IF 2.6) Pub Date : 2024-08-16 Ning Xia, Qing-Hai Chen, Zhao-Jun Meng, Shu-Yue Ma, Jia-Li Huang, Rong Shen, Yu-Tong Dong, Hai-Wei Du, Kun Zhou
Isobavachin (IBA) is a dihydroflavonoid compound with various pharmacological effects. However, further investigation into the hepatotoxicity of IBA is necessary. This study aims to identify the hepatotoxic effects of IBA and explore its potential mechanisms. The study assessed the impact of IBA on the viability of AML12, HepG2, LO2, rat, and mouse primary hepatocytes using MTT and LDH assays. Autophagy
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Ex vivo skin diffusion and decontamination studies of titanium dioxide nanoparticles Toxicol. In Vitro (IF 2.6) Pub Date : 2024-08-13 Adeline Tarantini, Emilie Jamet-Anselme, Sabine Lam, Vincent Haute, David Suhard, Nathalie Valle, Véronique Chamel-Mossuz, Céline Bouvier-Capely, Guillaume Phan
This study aims to adapt an experimental model based on Franz diffusion cells and porcine skin explants to characterize the diffusion of TiO NPs and to compare the efficacy of different cleansing products, soapy water and a calixarene cleansing nanoemulsion compared with pure water, as a function of the time of treatment.
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Photodynamic therapy with curcumin and near-infrared radiation as an antitumor strategy to glioblastoma cells Toxicol. In Vitro (IF 2.6) Pub Date : 2024-08-12 Marcelo Augusto Germani Marinho, Magno da Silva Marques, Camila de Oliveira Vian, Daza de Moraes Vaz Batista Filgueira, Ana Paula Horn
Glioblastoma is a malignant neoplasm that develops in the central nervous system and is characterized by high rates of cell proliferation and invasion, presenting resistance to treatments and a poor prognosis. Photodynamic therapy (PDT) is a therapeutic modality that can be applied in oncological cases and stands out for being less invasive. Photosensitizers (PS) of natural origin gained prominence
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Involvement of Sirt1-FoxO3a-Bnip3 axis and autophagy mediated mitochondrial turnover in according protection to hyperglycemic NRK-52E cells by Berberine Toxicol. In Vitro (IF 2.6) Pub Date : 2024-08-08 Sugandh Saxena, Sumit Kumar Anand, Ankita Sharma, Poonam Kakkar
Aberrant accumulation of dysfunctional mitochondria in renal cells during hyperglycemia signifies perturbed autophagy and mitochondrial turnover. This study aims to focus on the underlying mechanism involved in autophagy and mitophagy inducing efficacy of Berberine (isoquinoline alkaloid) in hyperglycemic NRK-52E cells. Berberine mediated protection to hyperglycemic cells prevented alteration in mitochondrial
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In vitro impacts of polystyrene microplastics and environmental pollutants on ethoxyresorufin-O-deethylase and glutathione S-transferase activity in Mossambica tilapia Toxicol. In Vitro (IF 2.6) Pub Date : 2024-08-05 Yan Yin, Haiyan Wang, Guijing Ouyang, Daxiong Han
Microplastic (MP) pollution is a potential threat to marine organisms. toxicity of MPs and other pollutants, such as pharmaceutically active compounds (PhACs) and brominated flame retardants (BFRs), has been understudied. This study aimed to investigate the effects of polystyrene microplastics (PS-MPs) with different particle sizes on two biomarkers: ethoxyresorufin-O-deethylase (EROD) and glutathione
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Estrogen receptor/androgen receptor transcriptional activation of benzophenone derivatives and integrated approaches to testing and assessment (IATA) for estrogenic effects Toxicol. In Vitro (IF 2.6) Pub Date : 2024-07-31 Handule Lee, Juyoung Park, Darlene M. Ortiz, Kwangsik Park
Estrogen receptor (ER) and androgen receptor (AR) transactivation assays for the benzophenone compounds (BPs) were performed using hERα-HeLa-9903 cells for ER and MMTV/22Rv1_GR-KO cells for AR. Results showed that some BPs, such as BP-1, BP-2, 4OH-BP, 4DHB, and 4-MBP, showed agonistic activity on ER with a higher RPCmax than 1 nM 17-β estradiol. The other BPs (BP, BP-3, BP-6, BP-7, and BP-8) showed
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Streptococcus mitis enhances metal-induced apoptosis in reconstructed human gingiva but not skin Toxicol. In Vitro (IF 2.6) Pub Date : 2024-07-28 Yan Zhang, Lin Shang, Sanne Roffel, Sander W. Spiekstra, Dongmei Deng, Susan Gibbs
Commensal bacteria colonizing oral mucosa and skin play an essential role in maintaining host-microbiome homeostasis. It is unknown whether cytotoxicity resulting from metal ions leaching from medical devices may be influenced by commensal microbes. Determine whether the extent of apoptosis triggered by nickel or titanium ions is influenced by and whether apoptosis occurs via the intrinsic or extrinsic
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Study on percutaneous penetration of representative cosmetic ingredients in a baby wipe product in an in vitro diaper rash skin model Toxicol. In Vitro (IF 2.6) Pub Date : 2024-07-26 Ning Li, Peter Styczynski, Roger Gibb, Chuiying Li, Randy Marsh
Studying percutaneous penetration of various cosmetic ingredients through intact and compromised skin can provide insight on quantitative exposure assessment for baby products intended for diapered skin. We developed an model (tape-stripped human skin) designed to achieve the Trans-Epidermal Water Loss values measured in babies with various degrees of diaper dermatitis. Six reference compounds showed
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Investigating the mechanisms of action of thyroid disruptors: A multimodal approach that integrates in vitro and metabolomic analysis Toxicol. In Vitro (IF 2.6) Pub Date : 2024-07-26 Naïs Clavel Rolland, Fanny Graslin, Frédéric Schorsch, Thierry Pourcher, Olivier Blanck
The thyroid gland, a vital component of the endocrine system, plays a pivotal role in regulating metabolic processes, growth, and development. To better characterize thyroid system disrupting chemicals (TSDC), we followed the next-generation risk assessment approach, which further considers the mechanistic profile of xenobiotics. We combined targeted testing with untargeted metabolomics. Four known
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Pro-inflammatory and genotoxic responses by metal oxide nanomaterials in alveolar epithelial cells and macrophages in submerged condition and air-liquid interface: An in vitro-in vivo correlation study Toxicol. In Vitro (IF 2.6) Pub Date : 2024-07-25 Emilio Di Ianni, Johanna Samulin Erdem, Shan Narui, Håkan Wallin, Iseult Lynch, Ulla Vogel, Nicklas Raun Jacobsen, Peter Møller
Studies on in vitro-in vivo correlations of inflammatory and genotoxic responses are needed to advance new approach methodologies. Here, we assessed pro-inflammatory and genotoxic responses by 13 nanosized metal oxides (nMeOx) and quartz (DQ12) in alveolar epithelial cells (A549) and macrophages (THP-1a) exposed in submerged conditions, and in A549:THP-1a co-cultures in air-liquid interface (ALI) system
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In vitro cytotoxicity of six per- and polyfluoroalkyl substances (PFAS) in human immune cell lines Toxicol. In Vitro (IF 2.6) Pub Date : 2024-07-22 Jennifer R. McCall, Kathryn T. Sausman, Ariel P. Brown, Ralph N. Mead
- and Polyfluoroalkyl substances (PFAS) are a group of persistent long-lived chemicals with global environmental contamination. The published literature is rife with confusing and sometimes contradictory effects of PFAS on animal and cell models, as well as epidemiological studies. Cytotoxicity studies are often used as an early indicator to guide safety requirements, regulation, and further studies
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Knowledge infrastructure for integrated data management and analysis supporting new approach methods in predictive toxicology and risk assessment Toxicol. In Vitro (IF 2.6) Pub Date : 2024-07-22 Barry Hardy, Tomaz Mohoric, Thomas Exner, Joh Dokler, Maja Brajnik, Daniel Bachler, Ody Mbegbu, Nora Kleisli, Lucian Farcal, Krzysztof Maciejczuk, Haris Rašidagić, Ghada Tagorti, Pascal Ankli, Daniel Burgwinkel, Divanshu Anand, Ugis Sarkans, Awais Athar
The EU-ToxRisk project (2016–2021) was a large European project working towards shifting toxicological testing away from animal tests, towards a toxicological assessment based on comprehensive mechanistic understanding of cause-consequence relationships of chemical adverse effects. More than 40 partners from scientific institutions, industry and regulators coordinated their work towards this goal in
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Nrf2 affects DNA damage repair and cell apoptosis through regulating HR and the intrinsic Caspase-dependent apoptosis pathway in TK6 cells exposed to hydroquinone Toxicol. In Vitro (IF 2.6) Pub Date : 2024-07-18 Lin Chen, Pu Guo, Lu Zhai, Lingxue Yu, Delong Zhu, Xiaoyi Hu, Zhuanzhuan Li, Yuting Chen, Qian Sun, Lei Sun, Hao Luo, Huanwen Tang
Hydroquinone (HQ) is one of benzene metabolites that can cause oxidative stress damage and Homologous recombination repair (HR). A good deal of reactive oxygen species (ROS) generated by oxidative stress can trigger apoptotic signaling pathways. The nuclear factor erythroid 2-related factor 2 (Nrf2) can regulate the cell response to oxidative stress damage. The aim of this study was to explore whether
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Unravelling the toxicity of carbon nanomaterials – From cellular interactions to mechanistic understanding Toxicol. In Vitro (IF 2.6) Pub Date : 2024-07-17 Bveledzani P. Makhado, Adewale O. Oladipo, Nozipho N. Gumbi, Lueta A. De Kock, Charlene Andraos, Mary Gulumian, Edward N. Nxumalo
The application of carbon nanomaterials in diverse fields has substantially increased their demand for commercial usage. Within the earliest decade, the development of functional materials has further increased the significance of this element. Despite the advancements recorded, the potential harmful impacts of embracing carbon nanomaterials for biological applications must be balanced against their
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Engineering of gelatin scaffold by extracellular matrix of Sertoli cells for embryonic stem cell proliferation Toxicol. In Vitro (IF 2.6) Pub Date : 2024-07-17 Zahra Keshtmand, Samane Eftekhari, Behnoosh Khodadadi, Bahare Farzollahi, Sadjad Khosravimelal, Syed Ataollah Shandiz, Mohammad Tavakkoli Yaraki
Mimicking the microenvironment of seminiferous tubules plays an indispensable role in directing differentiation of stem cells toward germ cells . In this work, we fabricated electrospun gelatin (EG) mats (, with diameter <500 nm) conditioned with Sertoli cells' extracellular matrix (ECM) to simulate both 3D structures and composition of normal testis tissue. Sertoli cells were isolated from mice testis
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Natural pyrethrins induced developmental toxicity of zebrafish swim bladder in vivo and genotoxicity of lung cells in vitro Toxicol. In Vitro (IF 2.6) Pub Date : 2024-07-16 Ruirui Li, Cheng Zhang, Wenping Xu, Liming Tao, Jiagao Cheng, Zhong Li, Yang Zhang
Natural pyrethrins (NPs) are insecticidal compounds isolated and extracted from pyrethrum flowers and are primarily use to control sanitary pests. The lungs become the main target after exposure, and its use may pose potential hazards to respiratory health. Therefore, in this paper, the toxic effects of NPs on human lung cells A549 were investigated and the risk of respiratory toxicity of NPs was studied
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Impact of high (1950 MHz) and extremely low (50 Hz) frequency electromagnetic fields on DNA damage caused by occupationally relevant exposures in human derived cell lines Toxicol. In Vitro (IF 2.6) Pub Date : 2024-07-16 Nadine Worel, Miroslav Mišík, Michael Kundi, Franziska Ferk, Hans-Peter Hutter, Armen Nersesyan, Georg Wultsch, Georg Krupitza, Siegfried Knasmueller
Epidemiological studies indicate that electromagnetic fields (EMF) are associated with cancer in humans. Exposure to mobile phone specific high frequency fields (HF-EMF) may lead to increased glioma risks, while low frequency radiation (LF-EMF) is associated with childhood leukemia. We studied the impact of HF-EMF (1950 MHz, UMTS signal) on DNA stability in an astrocytoma cell line (1321N1), and the
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Modulation of adipogenesis and lipogenesis by indomethacin and pantoprazole Toxicol. In Vitro (IF 2.6) Pub Date : 2024-07-14 Bita Entezari, Hasan Akbaba, Hande Gurer-Orhan
Endocrine disruptors are suggested to act as potential “obesogens” by interacting with various metabolic processes in adipose tissue. Besides industrial chemicals that are blamed for acting as endocrine disruptors as well as obesogens, pharmaceuticals can also cause obesogenic effects as unintended adverse effects. However, limited studies evaluated the obesogenic adverse effects of pharmaceuticals
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Integrated transcriptomic and metabolomic analysis reveals the underlying mechanisms for male reproductive toxicity of polystyrene nanoplastics in mouse spermatocyte-derived GC-2spd(ts) cells Toxicol. In Vitro (IF 2.6) Pub Date : 2024-07-11 Hang Han, Zhen Zhang, Bo Xu, Liyang Ding, Hong Yang, Tiantian He, Xing Du, Xiuying Pei, Xufeng Fu
Polystyrene nanoplastics (PS-NPs), are ubiquitous pollution sources in human environments, posing significant biosafety and health risks. While recent studies, including our own, have illustrated that PS-NPs can breach the blood-testis barrier and impact germ cells, there remains a gap in understanding their effects on specific spermatogenic cells such as spermatocytes. Herein, we employed an integrated
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The recovery from taxane mediated apoptosis in PC-3 castration-resistant metastatic prostate cancer cells Toxicol. In Vitro (IF 2.6) Pub Date : 2024-07-10 Gamze Guney Eskiler, Asuman Deveci Ozkan, Merve Acikel Elmas, Melek Ozturk, Serap Arbak
Here, we revealed the reversibility of cabazitaxel (CBZ)-induced apoptosis in PC-3 castration resistant metastatic prostate cancer cells (mCRPC) through the hallmarks of apoptosis. The recovery of PC-3 cells from apoptosis upon removal of CBZ at different recovery periods was evaluated by Annexin V, DNA damage, oxidative damage, mitochondrial membrane depolarization, and caspase activation. Our results
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QSAR modeling for cytotoxicity of sulfur-containing Shikonin oxime derivatives targeting HCT-15, MGC-803, BEL-7402, and MCF-7 cell lines Toxicol. In Vitro (IF 2.6) Pub Date : 2024-07-10 Abderrahim Diane, Salima Ben Tahar, Abdennacer El Mrabet, Reda Rabie, Taoufiq Saffaj, Bouchaib Ihssane
Targeting cancer cells through drug-based treatment or combination therapy protocols involving chemical compounds can be challenging due to multiple factors, including their resistance to bioactive compounds and the potential of drugs to damage healthy cells. This study aims to investigate the relationship between the structure of novel sulfur-containing shikonin oxime compounds and the corresponding
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Toxicity of New Psychoactive Substance (NPS): Threo-4-methylmethylphenidate (4-Mmph) – Prediction of toxicity using in silico methods Toxicol. In Vitro (IF 2.6) Pub Date : 2024-07-06 Łukasz Niżnik, Karolina Jabłońska, Michał Orczyk, Martyna Orzechowska, Joanna Toporowska-Kaźmierak, Marta Sowińska, Judyta Jasińska, Kamil Jurowski
This study represents the first application of methods to evaluate the toxicity of 4-methylphenidate (4-Mmph), a new psychoactive substance (NPS). Using advanced toxicology tools, it was feasible to anticipate key aspects of 4-Mmph's toxicological profile, including acute toxicity (LD), genotoxicity, cardiotoxicity, and possible endocrine disruption. The findings indicate significant acute toxicity
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Aerosol exposure at air-liquid-interface (AE-ALI) in vitro toxicity system characterisation: Particle deposition and the importance of air control responses Toxicol. In Vitro (IF 2.6) Pub Date : 2024-07-05 Alison Buckley, Chang Guo, Adam Laycock, Xianjin Cui, Marie-France Belinga-Desaunay-Nault, Eugenia Valsami-Jones, Martin Leonard, Rachel Smith
Experimental systems allowing aerosol exposure (AE) of cell cultures at the air-liquid-interface (ALI) are increasingly being used to assess the toxicity of inhaled contaminants as they are more biomimetic than standard methods using submerged cultures, however, they require detailed characterisation before use. An AE-ALI system combining aerosol generation with a CULTEX® exposure chamber was characterised
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Evaluating the combined and individual cytotoxic effect of beauvericin, enniatin B and ochratoxin a on breast cancer cells, leukemia cells, and fresh peripheral blood mononuclear cells Toxicol. In Vitro (IF 2.6) Pub Date : 2024-07-05 Ana Juan-García, Ana-María Ilie, Cristina Juan, Lola Martínez
Beauvericin (BEA), Enniatin B (ENN B), and Ochratoxin A (OTA) are mycotoxins produced by fungi . Their main effect on several organs and systems is associated with chronic exposure going from immunotoxicity, estrogenic disorders, and renal failure to cancer (in animals and humans). OTA belongs to Group 1 according to the International Agency for Research in Cancer (IARC) and it has legislated limited
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Predicting the binding affinity of chemicals to estrogen receptor using Hansen solubility parameters Toxicol. In Vitro (IF 2.6) Pub Date : 2024-07-05 Nobuyuki Fujiwara, Len Ito
Receptor-binding tests for the receptors of various substances are widely employed to identify drug candidates and predict the biological effects of chemical substances. Here, the results of chemicals binding to estrogen receptor (ER) reported in a validation study of the Organization for Economic Cooperation and Development TG 455 and the Hansen solubility parameter (HSP) values of the test substances
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Selected phytocannabinoids inhibit SN-38- and cytokine-evoked increases in epithelial permeability and improve intestinal barrier function in vitro Toxicol. In Vitro (IF 2.6) Pub Date : 2024-06-29 Dylan T. Marsh, Scott D. Smid
Irinotecan use is linked to the development of gastrointestinal toxicity and inflammation, or gastrointestinal mucositis. Selected phytocannabinoids have been ascribed anti-inflammatory effects in models of gastrointestinal inflammation, associated with maintaining epithelial barrier function. We characterised the mucoprotective capacity of the phytocannabinoids: cannabidiol, cannabigerol, cannabichromene
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Human in vitro percutaneous absorption of bisphenol S: Assessment of the skin reservoir and occlusion effects Toxicol. In Vitro (IF 2.6) Pub Date : 2024-06-28 Fabrice Marquet, Catherine Champmartin, Claire Seiwert, Matthieu Aubertin, Stéphane Viton, Lisa Chedik, Frédéric Cosnier
Bisphenol S (BPS) was introduced in many industrial and commercial applications as a presumed safer alternative to bisphenol A. However, concerns have been raised surrounding skin absorption and potential persistence of BPS and its related toxic effects in humans. A previous study revealed the likelihood of a reservoir building up in exposed skin. Here, we studied the interactions of BPS solubilized
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CircPDSS1 (hsa_circ_0017998) silencing induces ferroptosis in non-small-cell lung cancer cells by modulating the miR-137/SLC7A11/GPX4/GCLC axis Toxicol. In Vitro (IF 2.6) Pub Date : 2024-06-28 Ling Wu, Ni Li, Linwen Zhu, Guofeng Shao
Circular RNAs (circRNAs) regulate the tumorigenesis of non-small-cell lung cancer (NSCLC). CircPDSS1 (hsa_circ_0017998) has been newly discovered, and its role in NSCLC remains elusive. We aimed to investigate the functional roles and downstream targets of circPDSS1 in NSCLC cells. Cellular viabilities were measured through the Cell Counting Kit-8 (CCK-8) assay, whereas cell death was assessed through
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Selective inhibition of HDAC6 by N-acylhydrazone derivative reduces the proliferation and induces senescence in carcinoma hepatocellular cells Toxicol. In Vitro (IF 2.6) Pub Date : 2024-06-28 Guilherme Álvaro Ferreira-Silva, Daniel Alencar Rodrigues, Carolina Girotto Pressete, Ester Siqueira Caixeta, Angel Mauricio Castro Gamero, Marta Miyazawa, João Adolfo Costa Hanemann, Carlos Alberto Manssour Fraga, Alexandre Ferro Aissa, Marisa Ionta
Hepatocellular carcinoma (HCC) is a significant contributor to cancer-related deaths globally. Systemic therapy is the only treatment option for HCC at an advanced stage, with limited therapeutic response. In this study, we evaluated the antitumor potential of four -acylhydrazone (NAH) derivatives, namely , , , and , on HCC cell lines. We have previously demonstrated that the aforementioned NAH derivatives
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Three dimensional reconstruction of skin with melanoma: A model for study of invasion in vitro Toxicol. In Vitro (IF 2.6) Pub Date : 2024-06-25 Elenn Suzany Pereira Aranha, Leilane de Sousa Mendonça, Bianca de Lima Almeida, Emerson Lucena da Silva, Felipe Pantoja Mesquita, Emersom Silva Lima, Ana Paula Negreiros Nunes Alves, Maria Elisabete Amaral de Moraes, Raquel Carvalho Montenegro, Marne Carvalho de Vasconcellos
Melanoma is a type of tumor skin with high metastatic potential. Reconstructed human skin, development for pre-clinic assay, are make using primary human cells, but with same limitations. The aim this study was to characterize a cell culture model, with structure similar to human skin containing melanoma cells entirely from cell lines. Reconstructed skin with melanoma were development using human fibroblasts
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Influence of alkylthio and arylthio derivatives of tert-butylquinone on the induction of DNA damage in a human hepatocellular carcinoma cell line (HepG2) Toxicol. In Vitro (IF 2.6) Pub Date : 2024-06-25 Jelena Djordjevic Aleksic, Stoimir Kolarević, Jovana Jovanović Marić, Margareta Kračun-Kolarević, Bojana Žegura, Alja Štern, Dušan Sladić, Irena Novaković, Branka Vuković-Gačić
The aim of this study was to investigate the effects of -butylquinone (TBQ) and its alkylthio and arylthio derivatives on DNA in vitro, using acellular and cellular test systems. Direct interaction with DNA was studied using the plasmid pUC19. Cytotoxic (MTS assay) and genotoxic (comet assay and γH2AX focus assays) effects, and their influence on the cell cycle were studied in the HepG2 cell line.
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Exploring the combined impact of cisplatin and copper-cysteamine nanoparticles through Chemoradiation: An in-vitro study Toxicol. In Vitro (IF 2.6) Pub Date : 2024-06-19 Mahsa Ejtema, Nahid Chegeni, Amanollah Zarei-Ahmady, Zeinab Salehnia, Masoumeh Shamsi, Sasan Razmjoo
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1H NMR spectroscopic characterisation of HepG2 cells as a model metabolic system for toxicology studies Toxicol. In Vitro (IF 2.6) Pub Date : 2024-06-19 Maren Jinks, Emily C. Davies, Berin A. Boughton, Samantha Lodge, Garth L. Maker
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Cannabidiol protects mouse hippocampal neurons from neurotoxicity induced by amyloid β-peptide25–35 Toxicol. In Vitro (IF 2.6) Pub Date : 2024-06-18 Karen del Carmen Barboza Salgado, Rosiene Gomes de Freitas Nascimento, Pedro José Fernandes Nunes Coelho, Laser Antonio Machado Oliveira, Katiane Oliveira Pinto Coelho Nogueira
Alzheimer's disease (AD), the most prevalent form of dementia worldwide, is a significant health concern, according to the World Health Organization (WHO). The neuropathological diagnostic criteria for AD are based on the deposition of amyloid-β peptide (Aβ) and the formation of intracellular tau protein tangles. These proteins are associated with several overlapping neurodegenerative mechanisms, including
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Construction of an in vitro simulated one compartment extravascular administration model and its comparison with classic in vitro administration model in copper chloride induced HepG2 cell death Toxicol. In Vitro (IF 2.6) Pub Date : 2024-06-18 Lailai Yan, Dawei Fu, Jie Chen, Mingmei Hao, Juanling Fu, Biyun Yao, Weidong Hao, Peng Zhao
In this study, we designed an in vitro administration device based on compartment model theory and utilized it to construct an in vitro simulated one compartment extravascular administration model of copper chloride. Within the range of 3.91–1000.00 μM, the measured concentration-time curves of the simulated one compartment extravascular administration model almost coincide with the corresponding theoretical
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Fangchinoline inhibits mouse oocyte meiosis by disturbing MPF activity Toxicol. In Vitro (IF 2.6) Pub Date : 2024-06-12 Shi-Cai Gao, Ming-Zhe Dong, Bing-Wang Zhao, Sai-Li Liu, Jia-Ni Guo, Si-Min Sun, Yuan-Yuan Li, Yuan-Hong Xu, Zhen-Bo Wang
Fangchinoline (FA) is an alkaloid derived from the traditional Chinese medicine Fangji. Numerous studies have shown that FA has a toxic effect on various cancer cells, but little is known about its toxic effects on germ cells, especially oocytes. In this study, we investigated the effects of FA on mouse oocyte maturation and its potential mechanisms. Our results showed that FA did not affect meiosis
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Effect of long-term inorganic arsenic exposure on erythropoietin production in vitro Toxicol. In Vitro (IF 2.6) Pub Date : 2024-06-12 Md. Anamul Haque, Akari Yoshimoto, Hiroshi Nakagawa, Kazuhiko Nishimura
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Transcription Factor yin-Yang 1 augments nucleoporin 93 oncogene activity and modulates bladder Cancer malignancy Toxicol. In Vitro (IF 2.6) Pub Date : 2024-06-08 Gang Zhang, Wei Wei, Shao Li, Jinyi Yang
This study aims to investigate the functional interplay between transcription factor YY1 and nucleoporin 93 (NUP93) in regulating the malignancy of bladder cancer cells. NUP93 expressions in bladder cancer tissues and normal counterparts were analyzed using a public dataset and clinical samples. NUP93 and Yin Yang 1 (YY1) mRNA expression and protein levels in T24 and RT4 cells were determined by Quantitative
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Amantadine mitigates the cytotoxic and genotoxic effects of doxorubicin in SH-SY5Y cells and reduces its mutagenicity Toxicol. In Vitro (IF 2.6) Pub Date : 2024-06-06 Solange Soares, Jayne Torres de Sousa, Fernanda Brião Menezes Boaretto, Juliana Bondan da Silva, Duani Maria dos Santos, Ana Letícia Hilario Garcia, Juliana da Silva, Ivana Grivicich, Jaqueline Nascimento Picada
Amantadine (AMA) is a useful drug in neuronal disorders, but few studies have been performed to access its toxicological profile. Conversely, doxorubicin (Dox) is a well-known antineoplastic drug that has shown neurotoxic effects leading to cognitive impairment. The aims of this study are to evaluate the cytotoxic, genotoxic, and mutagenic effects of AMA, as well as its possible protective actions
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(-)-Epigallocatechin gallate as an inhibitor of hemoglobin-catalyzed lipid oxidation: molecular mechanism of action and nutritional application Toxicol. In Vitro (IF 2.6) Pub Date : 2024-06-06 Jia-Xin Li, Naihao Lu, Rong Tian
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Hyperoside reduced particulate matter 2.5-induced endoplasmic reticulum stress and senescence in skin cells Toxicol. In Vitro (IF 2.6) Pub Date : 2024-06-06 Pincha Devage Sameera Madushan Fernando, Mei Jing Piao, Herath Mudiyanselage Udari Lakmini Herath, Kyoung Ah Kang, Chang Lim Hyun, Eui Tae Kim, Young Sang Koh, Jin Won Hyun
Particulate matter 2.5 (PM) causes skin aging, inflammation, and impaired skin homeostasis. Hyperoside, a flavanol glycoside, has been proposed to reduce the risk of diseases caused by oxidative stress. This study evaluated the cytoprotective potential of hyperoside against PM-induced skin cell damage. Cultured human HaCaT keratinocytes were pretreated with hyperoside and treated with PM. Initially
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Pristimerin exhibits anti-cancer activity by inducing ER stress and AKT/GSK3β pathway through increasing intracellular ROS production in human esophageal cancer cells Toxicol. In Vitro (IF 2.6) Pub Date : 2024-06-06 Wei-Bin Hu, Yi-Ting Liu, Jing Li, Ying Wang, Xuan-Zi Sun, Ming-Yu Hua, Xue-Ting Liu, Bei-Na Hui
Pristimerin (Pris), a bioactive triterpenoid compound extracted from the and families, has been reported to exhibit an anti-cancer property on various cancers. However, the effects of Pris on esophageal cancer are poorly investigated. This current study sought to explore the activity and underlying mechanism of Pris against human esophageal squamous cell carcinoma (ESCC) cells. We demonstrated that
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In vitro assessment of the genotoxic and cytotoxic effects of clarified açai (Euterpe oleracea MART) extract in a gastric cancer cell line (AGP01 cells). Toxicol. In Vitro (IF 2.6) Pub Date : 2024-06-06 Thiago S. Santos, Marcelo O. Bahia, Adriana C. Guimarães, Carolina R.T. Souza, Nilton A. Muto, Hervé Rogez, Rommel M.R. Burbano
( MART) is a fruit of great importance for the Amazon region in nutritional, cultural and socioeconomic terms. In recent years, has been the subject of several studies due to its beneficial properties for health, including effects against tumor cells. Therefore, the present work aimed to evaluate the genotoxic and cytotoxic effects of the clarified extract of juice in a human metastatic gastric cancer
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Predicting the potential risks posed by antidepressants as emerging contaminants in fish based on network pharmacological analysis Toxicol. In Vitro (IF 2.6) Pub Date : 2024-06-06 Jinru Zhao, Jian Gao, Sijia Ma, Xintong Chen, Jun Wang
This study conducted a network pharmacology-based analysis to simultaneously discern a broad spectrum of potential environmental risks and health effects of antidepressants, a common class of pharmaceutical emerging contaminants (PECs) possessing a complex pharmacological profile, and predict the adverse phenotypes potentially occurring in fish associated with exposure to antidepressants and their
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Effects of polystyrene nanoplastic size on zebrafish embryo development Toxicol. In Vitro (IF 2.6) Pub Date : 2024-06-06 Varissara Chantho, Siwapech Sillapaprayoon, Rattaporn Saenmuangchin, Jasmine Pongkasem, Kulwadee Theanngern, Fahriye Ceyda Dudak Şeker, Sasitorn Aueviriyavit, Wittaya Pimtong
Polystyrene nanoplastics (PS) require a comprehensive evaluation of their toxicity and potential risks to humans and the environment. The zebrafish model, a well-established animal model increasingly utilized for nanotoxicity assessments, was employed in this study. Our research aimed to explore the toxic effects of PS with sizes of 30, 100, 200, and 450 nm on zebrafish embryos. Exposure experiments
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Assessment of silver nanoparticles' antitumor effects: Insights into cell number, viability, and morphology of glioblastoma and prostate cancer cells Toxicol. In Vitro (IF 2.6) Pub Date : 2024-06-05 Isabel Cristina Gomes Santos, Michelle Lopes de Oliveira, Renata Carvalho Silva, Celso Sant'Anna
Silver nanoparticles (AgNPs) hold promise for cancer therapy. This study aimed to evaluate their impact on tumor and non-tumor cell number, viability, and morphology. Antitumor activity was tested on U-87MG (glioblastoma) and DU-145 (prostate cancer) cell lines. Treatment with AgNPs notably reached a reduction of U-87MG and DU-145 cell growth by 89.30% and 79.74%, respectively, resulting in slower