-
The spatiotemporal analysis of SARS-CoV-2 transmission in China since the termination of the dynamic zero-COVID policy. Virol. Sin. (IF 4.3) Pub Date : 2024-09-11 Jiaying Li,Jingqi Yang,Xiao Ding,Hangyu Zhou,Na Han,Aiping Wu
China's dynamic zero-COVID policy has effectively curbed the spread of SARS-CoV-2, while inadvertently creating immunity gaps within its population. Subsequent surges in COVID-19 cases linked to various SARS-CoV-2 lineages post-policy termination necessitate a thorough investigation into the epidemiological landscape. This study addresses this issue by analyzing a comprehensive dataset of 39,456 high-quality
-
A multi-center study on genetic variations in the fusion protein of respiratory syncytial virus from children with Acute Lower Respiratory Tract Infections in China during 2017-2021. Virol. Sin. (IF 4.3) Pub Date : 2024-09-10 Yiliang Fu,Fei Li,Yun Zhu,Luci Huang,Qiuping Li,Hanwen Zhang,Lili Zhong,Hailin Zhang,Zheng-Xiu Luo,Gen Lu,Jikui Deng,Lingfeng Cao,Ying Wu,Rong Jin,Lei Li,Lili Xu,Xiangpeng Chen,Zhengde Xie
Respiratory syncytial virus (RSV) is a significant cause of acute lower respiratory tract infection (ALRTI) in children under five years of age. Between 2017 and 2021, 396 complete sequences of the RSV F gene were obtained from 500 RSV-positive throat swabs collected from ten hospitals across nine provinces in China. In addition, 151 sequences from China were sourced from GenBank and GISAID, making
-
Bis-benzylisoquinoline alkaloids inhibit flavivirus entry and replication by compromising endolysosomal trafficking and autophagy. Virol. Sin. (IF 4.3) Pub Date : 2024-09-07 Lihong Huang,Lele Liu,Junhai Zhu,Nanjun Chen,Jie Chen,Chuen-Fuk Chan,Fei Gao,Youqin Yin,Jiufeng Sun,Rongxin Zhang,Kehui Zhang,Wenbao Qi,Jianbo Yue
Flaviviruses, such as dengue virus (DENV), Zika virus (ZIKV), and Japanese encephalitis virus (JEV), represent a substantial public health challenge as there are currently no approved treatments available. Here, we investigated the antiviral effects of bis-benzylisoquinoline alkaloids (BBAs) on flavivirus infections. We evaluated five specific BBAs-berbamine, tetrandrine, iso-tetrandrine, fangchinoline
-
Antigenic analysis of the influenza B virus hemagglutinin protein. Virol. Sin. (IF 4.3) Pub Date : 2024-09-02 Mengyi Zhang,Chaoying Yang,Xi Wu,Yifei Wang,Lijie Wang,Qianqian Cui,Jincheng Tong,Yimeng An,Meina Cai,Shishi Cheng,Qi Jiang,Yulin Wang,Chenyan Zhao,Youchun Wang,Weijin Huang
Influenza B viruses (IBVs) primarily infect humans and are a common cause of respiratory infections in humans. Here, to systematically analyze the antigenicity of the IBVs Hemagglutinin (HA) protein, 31 B/Victoria and 19 B/Yamagata representative circulating strains were selected from Global Initiative of Sharing All Influenza Data (GISAID), and pseudotyped viruses were constructed with the vesicular
-
Rapid diagnosis of a fox's death case using nanopore sequencing reveals the infection with an Artic-like rabies virus. Virol. Sin. (IF 4.3) Pub Date : 2024-08-27 Yuhang Liu,Zhiqiang Liu,Jian Li,Xiaomin Yan,Weidi Xu,Le Yi,Changchun Tu,Biao He
-
The 2024 National Symposium on Insect Virology held in Qingdao. Virol. Sin. (IF 4.3) Pub Date : 2024-08-27 Jiali Si,Xi Wang,Manli Wang,Zhihong Hu
-
Bibliometric Analysis of Virology Advancements in the 21st Century. Virol. Sin. (IF 4.3) Pub Date : 2024-08-23 Lili Ma,Wei Pan,Jiali Si
-
The unique immune evasion mechanisms of the mpox virus and their implication for developing new vaccines and immunotherapies Virol. Sin. (IF 4.3) Pub Date : 2024-08-22 Dong Fang, Yan Liu, Dou Dou, Bin Su
Mpox is an infectious and contagious zoonotic disease caused by the mpox virus (MPXV), which belongs to the genus . Since 2022, MPXV has posed a significant threat to global public health. The emergence of thousands of cases across the Western Hemisphere prompted the World Health Organization to declare an emergency. The extensive coevolutionary history of poxviruses with humans has enabled these viruses
-
Trivalent SARS-CoV-2 virus-like particle vaccines exhibit broad-spectrum neutralization and protection against XBB.1 and BA.2.86 variants Virol. Sin. (IF 4.3) Pub Date : 2024-08-22 Lu Zhang, Siyu Tian, Jun Dai, Yuanyuan Li, Yu Zhou, Yan Li, Jiao Xu, Shuyun Liu, Zhiwei Lin, Zhaoyong Zhang, Jiantao Chen, Peilan Wei, Jingxian Zhao, Jing Jin, Yanqun Wang, Jincun Zhao
-
Antiviral activity of vitamin D derivatives against severe fever with thrombocytopenia syndrome virus in vitro and in vivo Virol. Sin. (IF 4.3) Pub Date : 2024-08-20 Chongda Luo, Xintong Yan, Shaokang Yang, Sichen Ren, Yan Luo, Jiazheng Li, Ping Wang, Yunfeng Shao, Wei Li, Song Li, Jingjing Yang, Ruiyuan Cao, Wu Zhong
Severe fever with thrombocytopenia syndrome virus (SFTSV) is a tick-borne virus that causes the severe fever thrombocytopenia syndrome, which manifests as fever and haemorrhage, accompanied by severe neurological complications. To date, no specific antiviral drugs have been approved for this indication. Herein, we investigated whether vitamin D derivatives inhibit SFTSV both and . An study demonstrated
-
Single-cell RNA-sequencing reveals a profound immune cell response in human cytomegalovirus-infected humanized mice Virol. Sin. (IF 4.3) Pub Date : 2024-08-15 An Wang, Xiao-Xu Zhu, Yuanyuan Bie, Bowen Zhang, Wenting Ji, Jing Lou, Muhan Huang, Xi Zhou, Yujie Ren
Human cytomegalovirus (HCMV) is a common herpesvirus that persistently infects a large portion of the world's population. Despite the robust host immune response, HCMV is able to replicate, evade host defenses, and establish latency throughout the lifespan by developing multiple immunomodulatory strategies, making the studies on the interaction between HCMV infection and host response particularly
-
Identification of mutations in viral proteins involved in cell adaptation using a reverse genetic system of the live attenuated hepatitis A virus vaccine H2 strain Virol. Sin. (IF 4.3) Pub Date : 2024-08-14 Xiu-Li Yan, Jian Li, Qing-Qing Ma, Hong-Jiang Wang, Lin Li, Hui Zhao, Cheng-Feng Qin, Xiao-Feng Li
The live attenuated hepatitis A virus vaccine H2 strain was developed by passaging a wild-type H2w isolate in cell cultures. Currently, the mechanism underlying its attenuation phenotype remain largely unknown. In this study, we generated a full-length infectious cDNA clone of the H2 strain using in-fusion techniques. The recovered H2 strain (H2ic) from the cDNA clone exhibited an efficient replication
-
A bivalent Delta/BA.5 mRNA vaccine elicits broad immune responses against various lineages of SARS-CoV-2 including JN.1 Virol. Sin. (IF 4.3) Pub Date : 2024-08-08 Kangyin Li, Qi Liu, Yan Wu, Bihao Wu, Shaohong Chen, Xinghai Zhang, Xiaoying Jia, Rui Gong, Yucai Peng, Huajun Zhang, Sandra Chiu
-
Antibacterial activity evaluation of a novel K3-specific phage against Acinetobacter baumannii and evidence for receptor-binding domain transfer across morphologies Virol. Sin. (IF 4.3) Pub Date : 2024-08-03 Xiangkuan Zheng, Meihan Liu, Pei Li, Sixiang Xu, Long Chen, Guoxin Xu, Xiaoxiao Pang, Hong Du, Yishan zheng, Xiang Huo, Zhongming Tan, Juan Li, Zhirong Li, Wei Zhang
() poses a serious public health challenge due to its notorious antimicrobial resistance, particularly carbapenem-resistant (CRAB). In this study, we isolated a virulent phage, named P1068, from medical wastewater capable of lysing CRAB, primarily targeting the K3 capsule type. Basic characterization showed that P1068 infected the ZWAb014 with an optimal MOI of 1, experienced a latent period of 10 min
-
Identification of a novel caspase cleavage motif AEAD Virol. Sin. (IF 4.3) Pub Date : 2024-08-03 Yujie Fang, Zhou Gong, Miaomiao You, Ke Peng
Infections of many viruses induce caspase activation to regulate multiple cellular pathways, including programmed cell death, immune signaling and etc. Characterizations of caspase cleavage sites and substrates are important for understanding the regulation mechanisms of caspase activation. Here, we identified and analyzed a novel caspase cleavage motif AEAD, and confirmed its caspase dependent cleavage
-
Circulation patterns and molecular characteristics of respiratory syncytial virus among hospitalized children in Tianjin, China, before and during the COVID-19 pandemic (2017–2022) Virol. Sin. (IF 4.3) Pub Date : 2024-07-26 Mengzhu Hou, Guangping Liu, Chao Meng, Lili Dong, Yulian Fang, Lu Wang, Ning Wang, Chunquan Cai, Hanjie Wang
Respiratory syncytial virus (RSV) is the main pathogen that causes hospitalization for acute lower respiratory tract infections (ALRIs) in children. With the reopening of communities and schools, the resurgence of RSV in the COVID-19 post-pandemic era has become a major concern. To understand the circulation patterns and genotype variability of RSV in Tianjin before and during the COVID-19 pandemic
-
Baicalein suppresses Coxsackievirus B3 replication by inhibiting caspase-1 and viral protease 2A Virol. Sin. (IF 4.3) Pub Date : 2024-07-16 Yanyan Dong, Enze Shao, Siwei Li, Ruiqi Wang, Dan Wang, Lixin Wang, Hong Yang, Yingxia He, Tian Luan, Yang Chen, Yao Wang, Lexun Lin, Yan Wang, Zhaohua Zhong, Wenran Zhao
Myocarditis is an inflammatory disease of the cardiac muscle and one of the primary causes of dilated cardiomyopathy. Group B coxsackievirus (CVB) is one of the leading causative pathogens of viral myocarditis, which primarily affects children and young adults. Due to the lack of vaccines, the development of antiviral medicines is crucial to controlling CVB infection and the progression of myocarditis
-
A quadrivalent norovirus vaccine based on a chimpanzee adenovirus vector induces potent immunity in mice Virol. Sin. (IF 4.3) Pub Date : 2024-07-10 Yihua Jiang, Lingjin Sun, Nan Qiao, Xiang Wang, Caihong Zhu, Man Xing, Hui Liu, Ping Zhou, Dongming Zhou
Norovirus (NoV) infection is a major cause of gastroenteritis worldwide. The virus poses great challenges in developing vaccines with broad immune protection due to its genetic and antigenic diversity. To date, there are no approved NoV vaccines for clinical use. Here, we aimed to develop a broad-acting quadrivalent NoV vaccine based on a chimpanzee adenovirus vector, AdC68, carrying the major capsid
-
Structural and functional insights into the 2′-O-methyltransferase of SARS-CoV-2 Virol. Sin. (IF 4.3) Pub Date : 2024-07-03 Jikai Deng, Feiyu Gong, Yingjian Li, Xue Tan, Xuemei Liu, Shimin Yang, Xianying Chen, Hongyun Wang, Qianyun Liu, Chao Shen, Li Zhou, Yu Chen
A unique feature of coronaviruses is their utilization of self-encoded nonstructural protein 16 (nsp16), 2′-O-methyltransferase (2′-O-MTase), to cap their RNAs through ribose 2′-O-methylation modification. This process is crucial for maintaining viral genome stability, facilitating efficient translation, and enabling immune escape. Despite considerable advances in the ultrastructure of SARS-CoV-2 nsp16/nsp10
-
Measures of insulin resistance and beta cell function before and after treatment of HCV infection Virol. Sin. (IF 4.3) Pub Date : 2024-06-29 Jizheng Chen, Pan Qiu, Tingfeng Zhao, Haowei Jiang, Kebinur Tursun, Sulaiman Ksimu, Xinwen Chen, Qian Wang
The association between chronic HCV infection and type 2 diabetes mellitus (T2DM) has been established; however, there is limited research on β-cell function particularly in the pre-diabetic population. Here, we evaluated indices of β-cell function and insulin sensitivity across the spectrum from normal glucose tolerance to T2DM in individuals with and without chronic hepatitis C (CHC), and the effects
-
AIMP2 restricts EV71 replication by recruiting SMURF2 to promote the degradation of 3D polymerase Virol. Sin. (IF 4.3) Pub Date : 2024-06-28 Junrui Ren, Lei Yu, Qiuhan Zhang, Pengyu Ren, Yumeng Cai, Xueyun Wang, Ke Lan, Shuwen Wu
Hand, foot and mouth disease (HFMD), mainly caused by enterovirus 71 (EV71), has frequently occurred in the Asia-Pacific region, posing a significant threat to the health of infants and young children. Therefore, research on the infection mechanism and pathogenicity of enteroviruses is increasingly becoming important. The 3D polymerase, as the most critical RNA-dependent RNA polymerase (RdRp) for EV71
-
Individual virome analysis reveals the general co-infection of mammal-associated viruses with SARS-related coronaviruses in bats Virol. Sin. (IF 4.3) Pub Date : 2024-06-28 Hao-Rui Si, Ke Wu, Jia Su, Tian-Yi Dong, Yan Zhu, Bei Li, Ying Chen, Yang Li, Zheng-Li Shi, Peng Zhou
Bats are the natural reservoir hosts for SARS-related coronavirus (SARSr-CoV) and other highly pathogenic microorganisms. Therefore, it is conceivable that an individual bat may harbor multiple microbes. However, there is limited knowledge on the overall co-circulation of microorganisms in bats. Here, we conducted a 16-year monitoring of bat viruses in south and central China and identified 238 SARSr-CoV
-
Coexistence of adeno-associated virus 2 with adenovirus 18 or herpesvirus may be associated with severe lingual papillomatosis in an immunocompromised individual Virol. Sin. (IF 4.3) Pub Date : 2024-06-22 Jie-Mei Yu, Ze-Yin Liang, Yuan-Hui Fu, Xiang-Lei Peng, Yan-Peng Zheng, Yu-Jun Dong, Jin-Sheng He
-
Continued evolution of H10N3 influenza virus with adaptive mutations poses an increased threat to mammals Virol. Sin. (IF 4.3) Pub Date : 2024-06-11 Shiping Ding, Jiangtao Zhou, Junlong Xiong, Xiaowen Du, Wenzhuo Yang, Jinyu Huang, Yi Liu, Lihong Huang, Ming Liao, Jiahao Zhang, Wenbao Qi
The H10 subtype avian influenza virus (AIV) poses an ongoing threat to both birds and humans. Notably, fatal human cases of H10N3 and H10N8 infections have drawn public attention. In 2022, we isolated two H10N3 viruses (A/chicken/Shandong/0101/2022 and A/chicken/Shandong/0603/2022) from diseased chickens in China. Genome analysis revealed that these viruses were genetically associated with human-origin
-
The binding and structural basis of fox ACE2 to RBDs from different sarbecoviruses Virol. Sin. (IF 4.3) Pub Date : 2024-06-10 Junsen Chen, Junqing Sun, Zepeng Xu, Linjie Li, Xinrui Kang, Chunliang Luo, Qi Wang, Xueyang Guo, Yan Li, Kefang Liu, Ying Wu
Foxes are susceptible to SARS-CoV-2 in laboratory settings, and there have also been reports of natural infections of both SARS-CoV and SARS-CoV-2 in foxes. In this study, we assessed the binding capacities of fox ACE2 to important sarbecoviruses, including SARS-CoV, SARS-CoV-2, and animal-origin SARS-CoV-2 related viruses. Our findings demonstrated that fox ACE2 exhibits broad binding capabilities
-
Detection of HBV DNA integration in plasma cell-free DNA of different HBV diseases utilizing DNA capture strategy Virol. Sin. (IF 4.3) Pub Date : 2024-06-07 Zerui Yang, Jingyan Zeng, Yueyue Chen, Mengchun Wang, Hongchun Luo, Ai-Long Huang, Haijun Deng, Yuan Hu
The landscape of hepatitis B virus (HBV) integration in the plasma cell-free DNA (cfDNA) of HBV-infected patients with different stages of liver diseases [chronic hepatitis B (CHB), liver cirrhosis (LC), and hepatocellular carcinoma (HCC)] remains unclear. In this study, we developed an improved strategy for detecting HBV DNA integration in plasma cfDNA, based on DNA probe capture and next-generation
-
Correlation between circulating T follicular helper cell levels after infection and a decreased risk of COVID-19 re-infection Virol. Sin. (IF 4.3) Pub Date : 2024-06-07 Jinzhu Feng, Zeyu Pu, Rong Li, Yuzhuang Li, Xuewen Qin, Hui Zhang, Yiwen Zhang
-
Identification of residues in Lassa virus glycoprotein 1 involved in receptor switch Virol. Sin. (IF 4.3) Pub Date : 2024-06-06 Jiao Guo, Yi Wan, Yang Liu, Xiaoying Jia, Siqi Dong, Gengfu Xiao, Wei Wang
Lassa virus (LASV) is an enveloped, negative-sense RNA virus that causes Lassa hemorrhagic fever. Successful entry of LASV requires the viral glycoprotein 1 (GP1) to undergo a receptor switch from its primary receptor alpha-dystroglycan (α-DG) to its endosomal receptor lysosome-associated membrane protein 1 (LAMP1). A conserved histidine triad in LASV GP1 has been reported to be responsible for receptor
-
Pseudorabies virus VHS protein abrogates interferon responses by blocking NF-κB and IRF3 nuclear translocation Virol. Sin. (IF 4.3) Pub Date : 2024-05-30 Zhenfang Yan, Jiayu Yue, Yaxin Zhang, Zhengyang Hou, Dianyu Li, Yanmei Yang, Xiangrong Li, Adi Idris, Huixia Li, Shasha Li, Jingying Xie, Ruofei Feng
Herpesviruses antagonize host antiviral responses through a myriad of molecular strategies culminating in the death of the host cells. Pseudorabies virus (PRV) is a significant veterinary pathogen in pigs, causing neurological sequalae that ultimately lead to the animal's demise. PRV is known to trigger apoptotic cell death during the late stages of infection. The virion host shutdown protein (VHS)
-
Diverse genotypes of norovirus genogroup I and II contamination in environmental water in Thailand during the COVID-19 outbreak from 2020 to 2022 Virol. Sin. (IF 4.3) Pub Date : 2024-05-30 Kattareeya Kumthip, Pattara Khamrin, Aksara Thongprachum, Rungnapa Malasao, Arpaporn Yodmeeklin, Hiroshi Ushijima, Niwat Maneekarn
Noroviruses (NoVs) are the most significant viral pathogens associated with waterborne and foodborne outbreaks of nonbacterial acute gastroenteritis in humans worldwide. This study aimed to investigate the prevalence and diversity of NoVs contaminated in the environmental water in Chiang Mai, Thailand. A total of 600 environmental water samples were collected from ten sampling sites in Chiang Mai from
-
Seroprevalence of neutralizing antibodies against HFMD associated enteroviruses among healthy individuals in Shanghai, China, 2022 Virol. Sin. (IF 4.3) Pub Date : 2024-05-25 Jiayu Wang, Wencheng Wu, Wei Wang, Jingyi Zhang, Jianwei Xiao, Mingyi Cai, Jiaying Guo, Run Li, Shuangli Zhu, Wanju Zhang, Min Chen, Zheng Teng
-
Calmodulin-like 5 promotes PEDV replication by regulating late-endosome synthesis and innate immune response Virol. Sin. (IF 4.3) Pub Date : 2024-05-23 Wen-Jun Tian, Xiu-Zhong Zhang, Jing Wang, Jian-Feng Liu, Fu-Huang Li, Xiao-Jia Wang
The infection caused by porcine epidemic diarrhea virus (PEDV) is associated with high mortality in piglets worldwide. Host factors involved in the efficient replication of PEDV, however, remain largely unknown. Our recent proteomic study in the virus-host interaction network revealed a significant increase in the accumulation of CALML5 (EF-hand protein calmodulin-like 5) following PEDV infection.
-
Function investigation of p11.5 in ASFV infection Virol. Sin. (IF 4.3) Pub Date : 2024-05-22 Dan Yin, Bin Shi, Renhao Geng, Yingnan Liu, Lang Gong, Hongxia Shao, Kun Qian, Hongjun Chen, Aijian Qin
Virus replication relies on complex interactions between viral proteins. In the case of African swine fever virus (ASFV), only a few such interactions have been identified so far. In this study, we demonstrate that ASFV protein p72 interacts with p11.5 using co-immunoprecipitation and liquid chromatography-mass spectrometry (LC-MS). It was found that protein p72 interacts specifically with p11.5 at
-
Development of a Měnglà virus minigenome and comparison of its polymerase complexes with those of other filoviruses Virol. Sin. (IF 4.3) Pub Date : 2024-05-21 Shi-Zhe Xie, Ke Yao, Bei Li, Cheng Peng, Xing-Lou Yang, Zheng-Li Shi
Ebola virus (EBOV) and Marburg virus (MARV), members of the family, are highly pathogenic and can cause hemorrhagic fevers, significantly impacting human society. Bats are considered reservoirs of these viruses because related filoviruses have been discovered in bats. However, due to the requirement for maximum containment laboratories when studying infectious viruses, the characterization of bat filoviruses
-
First complete genomic sequence analysis of canine distemper virus in wild boar Virol. Sin. (IF 4.3) Pub Date : 2024-05-18 Tong Wang, Haiying Du, Na Feng, Yuhang Liu, Yu Xu, Heting Sun, Peng Peng, Siyuan Qin, Xiaotian Zhang, Yan Liu, Mingyuan Yu, Hongrui Liang, Biao He, Guoqiang Zhu, Changchun Tu, Zhongzhong Tu
-
DNA vaccine prime and replicating vaccinia vaccine boost induce robust humoral and cellular immune responses against MERS-CoV in mice Virol. Sin. (IF 4.3) Pub Date : 2024-05-18 Xiuli Shen, Shuhui Wang, Yanling Hao, Yuyu Fu, Li Ren, Dan Li, Wenqi Tang, Jing Li, Ran Chen, Meiling Zhu, Shuo Wang, Ying Liu, Yiming Shao
As of December 2022, 2603 laboratory-identified Middle East respiratory syndrome coronavirus (MERS-CoV) infections and 935 associated deaths, with a mortality rate of 36%, had been reported to the World Health Organization (WHO). However, there are still no vaccines for MERS-CoV, which makes the prevention and control of MERS-CoV difficult. In this study, we generated two DNA vaccine candidates by
-
Genetic characteristics of H1N1 influenza virus outbreak in China in early 2023 Virol. Sin. (IF 4.3) Pub Date : 2024-05-18 Xuanxuan Li, Zefeng Dong, Jiaming Li, Chuanran Dou, Deyu Tian, Zhenghai Ma, Wenjun Liu, George F. Gao, Yuhai Bi
-
NSUN2 mediates distinct pathways to regulate enterovirus 71 replication Virol. Sin. (IF 4.3) Pub Date : 2024-05-18 Lishi Liu, Zhen Chen, Kui Zhang, Haojie Hao, Li Ma, Haizhou Liu, Baocheng Yu, Shuang Ding, Xueyan Zhang, Miao Zhu, Xiang Guo, Yi Liu, Haibin Liu, Fang Huang, Ke Peng, Wuxiang Guan
Increasing evidences suggest that the methyltransferase NSUN2 catalyzes 5-methylcytosine (m5C) modifications on viral RNAs, which are essential for the replication of various viruses. Despite the function of m5C deposition is well characterized, other potential roles of NSUN2 in regulating viral replication remain largely unknown. In this study, the m5C modified residues catalyzed by NSUN2 on enterovirus
-
Design of antiviral AGO2-dependent short hairpin RNAs Virol. Sin. (IF 4.3) Pub Date : 2024-05-09 Yuanyuan Bie, Jieling Zhang, Jiyao Chen, Yumin Zhang, Muhan Huang, Leike Zhang, Xi Zhou, Yang Qiu
The increasing emergence and re-emergence of RNA virus outbreaks underlines the urgent need to develop effective antivirals. RNA interference (RNAi) is a sequence-specific gene silencing mechanism that is triggered by small interfering RNAs (siRNAs) or short hairpin RNAs (shRNAs), which exhibits significant promise for antiviral therapy. AGO2-dependent shRNA (agshRNA) generates a single-stranded guide
-
Ten computational challenges in human virome studies Virol. Sin. (IF 4.3) Pub Date : 2024-04-30 Yifan Wu, Yousong Peng
In recent years, substantial advancements have been achieved in understanding the diversity of the human virome and its intricate roles in human health and diseases. Despite this progress, the field of human virome research remains nascent, primarily hindered by the lack of effective methods, particularly in the domain of computational tools. This perspective systematically outlines ten computational
-
Joint deletion of multifunctional MGF505-7R and H240R genes generates a safe and effective African swine fever virus attenuated live vaccine candidate Virol. Sin. (IF 4.3) Pub Date : 2024-04-30 Changjiang Weng, Li Huang, Guangqiang Ye
-
RNA viromes of Dermacentor nuttalli ticks reveal a novel uukuvirus in Qīnghăi Province, China Virol. Sin. (IF 4.3) Pub Date : 2024-04-26 Yaohui Fang, Jun Wang, Jianqing Sun, Zhengyuan Su, Shengyao Chen, Jian Xiao, Jun Ni, Zhihong Hu, Yubang He, Shu Shen, Fei Deng
Ticks are a major parasite on the Qīnghăi-Tibet Plateau, western China, and represent an economic burden to agriculture and animal husbandry. Despite research on tick-borne pathogens that threaten humans and animals, the viromes of dominant tick species in this area remain unknown. In this study, we collected ticks near Qīnghăi Lake and identified 13 viruses belonging to at least six families through
-
Characterization of humoral immune responses against SARS-CoV-2 accessory proteins in infected patients and mouse model Virol. Sin. (IF 4.3) Pub Date : 2024-04-26 Yuming Li, Yanhong Tang, Xiaoqian Wang, Airu Zhu, Dongdong Liu, Yiyun He, Hu Guo, Jie Zheng, Xinzhuo Liu, Fengyu Chi, Yanqun Wang, Zhen Zhuang, Zhaoyong Zhang, Donglan Liu, Zhao Chen, Fang Li, Wei Ran, Kuai Yu, Dong Wang, Liyan Wen, Jianfen Zhuo, Yanjun Zhang, Yin Xi, Jingxian Zhao, Jincun Zhao, Jing Sun
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of COVID-19, encodes several accessory proteins that have been shown to play crucial roles in regulating the innate immune response. However, their expressions in infected cells and immunogenicity in infected humans and mice are still not fully understood. This study utilized various techniques such as luciferase immunoprecipitation
-
Evolutionary dynamics and comparative pathogenicity of clade 2.3.4.4b H5 subtype avian influenza viruses, China, 2021–2022 Virol. Sin. (IF 4.3) Pub Date : 2024-04-26 Siru Lin, Junhong Chen, Ke Li, Yang Liu, Siyuan Fu, Shumin Xie, Aimin Zha, Aiguo Xin, Xinyu Han, Yuting Shi, Lingyu Xu, Ming Liao, Weixin Jia
The recent concurrent emergence of H5N1, H5N6, and H5N8 avian influenza viruses (AIVs) has led to significant avian mortality globally. Since 2020, frequent human-animal interactions have been documented. To gain insight into the novel H5 subtype AIVs (i.e., H5N1, H5N6 and H5N8), we collected 6102 samples from various regions of China between January 2021 and September 2022, and identified 41 H5Nx
-
Pseudorabies virus manipulates mitochondrial tryptophanyl-tRNA synthetase 2 for viral replication Virol. Sin. (IF 4.3) Pub Date : 2024-04-16 Xiu-Qing Li, Meng-Pan Cai, Ming-Yang Wang, Bo-Wen Shi, Guo-Yu Yang, Jiang Wang, Bei-Bei Chu, Sheng-Li Ming
The pseudorabies virus (PRV) is identified as a double-helical DNA virus responsible for causing Aujeszky's disease, which results in considerable economic impacts globally. The enzyme tryptophanyl-tRNA synthetase 2 (WARS2), a mitochondrial protein involved in protein synthesis, is recognized for its broad expression and vital role in the translation process. The findings of our study showed an increase
-
Risk of infection with arboviruses in a healthy population in Pakistan based on seroprevalence Virol. Sin. (IF 4.3) Pub Date : 2024-04-08 Shengyao Chen, Muhammad Saqib, Hafiz Sajid Khan, Yuan Bai, Usman Ali Ashfaq, Muhammad Khalid Mansoor, Abulimti Moming, Jing Liu, Min Zhou, Saifullah Khan Niazi, Qiaoli Wu, Awais-Ur-Rahman Sial, Shuang Tang, Muhammad Hassan Sarfraz, Aneela Javed, Sumreen Hayat, Mohsin Khurshid, Iahtasham Khan, Muhammad Ammar Athar, Zeeshan Taj, Bo Zhang, Fei Deng, Ali Zohaib, Shu Shen
Infectious diseases caused by arboviruses are a public health concern in Pakistan. However, studies on data prevalence and threats posed by arboviruses are limited. This study investigated the seroprevalence of arboviruses in a healthy population in Pakistan, including severe fever with thrombocytopenia syndrome virus (SFTSV), Crimean-Congo hemorrhagic fever virus (CCHFV), Tamdy virus (TAMV), and Karshi
-
Nanobodies against African swine fever virus p72 and CD2v proteins as reagents for developing two cELISAs to detect viral antibodies Virol. Sin. (IF 4.3) Pub Date : 2024-04-06 Jiahong Zhu, Qingyuan Liu, Liuya Li, Runyu Zhang, Yueting Chang, Jiakai Zhao, Siyu Liu, Xinyu Zhao, Xu Chen, Yani Sun, Qin Zhao
African swine fever virus (ASFV) poses a significant threat to the global swine industry. Currently, there are no effective vaccines or treatments available to combat ASFV infection in pigs. The primary means of controlling the spread of the disease is through rapid detection and subsequent elimination of infected pig. Recently, a lower virulent ASFV isolate with a deleted gene (CD2v-deleted) has been
-
Establishment and application of a surrogate model for human Ebola virus disease in BSL-2 laboratory Virol. Sin. (IF 4.3) Pub Date : 2024-03-29 Wanying Yang, Wujian Li, Wujie Zhou, Shen Wang, Weiqi Wang, Zhenshan Wang, Na Feng, Tiecheng Wang, Ying Xie, Yongkun Zhao, Feihu Yan, Xianzhu Xia
The Ebola virus (EBOV) is a member of the genus, family, which causes severe hemorrhagic diseases in humans and non-human primates (NHPs), with a case fatality rate of up to 90%. The development of countermeasures against EBOV has been hindered by the lack of ideal animal models, as EBOV requires handling in biosafety level (BSL)-4 facilities. Therefore, accessible and convenient animal models are
-
An optimized high-throughput SARS-CoV-2 dual reporter trans-complementation system for antiviral screening in vitro and in vivo Virol. Sin. (IF 4.3) Pub Date : 2024-03-26 Yingjian Li, Xue Tan, Jikai Deng, Xuemei Liu, Qianyun Liu, Zhen Zhang, Xiaoya Huang, Chao Shen, Ke Xu, Li Zhou, Yu Chen
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is still epidemic around the world. The manipulation of SARS-CoV-2 is restricted to biosafety level 3 laboratories (BSL-3). In this study, we developed a SARS-CoV-2 ΔN-GFP-HiBiT replicon delivery particles (RDPs) encoding a dual reporter gene, GFP-HiBiT, capable of producing both GFP signal and luciferase activities. Through optimal selection
-
A lethal mice model of recombinant vesicular stomatitis viruses for EBOV-targeting prophylactic vaccines evaluation Virol. Sin. (IF 4.3) Pub Date : 2024-03-24 Hong-Qing Zhang, Zhe-Rui Zhang, Cheng-Lin Deng, Zhi-Ming Yuan, Bo Zhang
-
Crucial role played by CK8+ cells in mediating alveolar injury remodeling for patients with COVID-19 Virol. Sin. (IF 4.3) Pub Date : 2024-03-21 Yufeng Li, Hengrui Hu, Jia Liu, Longda Ma, Xi Wang, Liang Liu, Qian Liu, Liang Ren, Jiang Li, Fei Deng, Zhihong Hu, Yiwu Zhou, Manli Wang
The high risk of SARS-CoV-2 infection and reinfection and the occurrence of post-acute pulmonary sequelae have highlighted the importance of understanding the mechanism underlying lung repair after injury. To address this concern, comparative and systematic analyses of SARS-CoV-2 infection in COVID-19 patients and animals were conducted. In the lungs of nine patients who died of COVID-19 and one recovered
-
Severe fever with thrombocytopenia syndrome virus infection attributed to cat contact: A case report in Beijing, China Virol. Sin. (IF 4.3) Pub Date : 2024-03-20 Shuo Zhang, Yu Leng, Fa-Xue Zhang, Bo-Kang Fu, Bing-Zheng Wu, Jia-Hao Chen, Ning Cui, Ning Zhao, Wei Liu, Hao Li
-
KIF5B-mediated internalization of FMDV promotes virus infection Virol. Sin. (IF 4.3) Pub Date : 2024-03-16 Wei Zhang, Fan Yang, Yang Yang, Weijun Cao, Wenhua Shao, Jiali Wang, Mengyao Huang, Zhitong Chen, Xiaoyi Zhao, Weiwei Li, Zixiang Zhu, Haixue Zheng
Foot-and-mouth disease (FMD) is a highly contagious and economically important disease, which is caused by the FMD virus (FMDV). Although the cell receptor for FMDV has been identified, the specific mechanism of FMDV internalization after infection remains unknown. In this study, we found that kinesin family member 5B (KIF5B) plays a vital role during FMDV internalization. Moreover, we confirmed the
-
Integration of HiBiT into enteroviruses: A universal tool for advancing enterovirus virology research Virol. Sin. (IF 4.3) Pub Date : 2024-03-16 Rui Yu, Xiaohong Li, Peng Zhang, Minghao Xu, Jitong Zhao, Jingjing Yan, Chenli Qiu, Jiayi Shu, Shuo Zhang, Miaomiao Kang, Xiaoyan Zhang, Jianqing Xu, Shuye Zhang
The utilization of enteroviruses engineered with reporter genes serves as a valuable tool for advancing our understanding of enterovirus biology and its applications, enabling the development of effective therapeutic and preventive strategies. In this study, our initial attempts to introduce a NanoLuc luciferase (NLuc) reporter gene into recombinant enteroviruses were unsuccessful in rescuing viable
-
Precore mutation enhances viral replication to facilitate persistent infection especially in HBeAg-negative patients Virol. Sin. (IF 4.3) Pub Date : 2024-03-14 Guixin Li, Danli Yang, Xin Liu, Ting Zhang, Hui Liu, Jun Zou, Zimeng Xu, Xiangmei Chen, Lizhong Dai, Hongsong Chen, Fengmin Lu
Naturally occurred precore (PC, G1896A) and/or basal core promoter (BCP, A1762T/G1764A) mutations are prevalent in chronic HBV-infected patients, especially those under HBeAg-negative status. However, the replicative capacity of HBV with PC/BCP mutations remains ambiguous. Herein, meta-analysis showed that, only under HBeAg-negative status, the serum HBV DNA load in patients with PC mutation was 7
-
The infection kinetics and transmission potential of two Guaico Culex viruses in Culex quinquefasciatus mosquitoes Virol. Sin. (IF 4.3) Pub Date : 2024-03-08 Ru-Yi Chen, Teng Zhao, Jing-Jing Guo, Feng Zhu, Na-Na Zhang, Xiao-Feng Li, Hai-Tao Liu, Fei Wang, Yong-Qiang Deng, Cheng-Feng Qin
Guaico Culex virus (GCXV) is a newly identified segmented Jingmenvirus from spp. mosquitoes in Central and South America. The genome of GCXV is composed of four or five single-stranded positive RNA segments. However, the infection kinetics and transmission capability of GCXV in mosquitoes remain unknown. In this study, we used reverse genetics to rescue two GCXVs (4S and 5S) that contained four and
-
Mast cell degranulation-triggered by SARS-CoV-2 induces tracheal-bronchial epithelial inflammation and injury Virol. Sin. (IF 4.3) Pub Date : 2024-03-06 Jian-Bo Cao, Shu-Tong Zhu, Xiao-Shan Huang, Xing-Yuan Wang, Meng-Li Wu, Xin Li, Feng-Liang Liu, Ling Chen, Yong-Tang Zheng, Jian-Hua Wang
SARS-CoV-2 infection-induced hyper-inflammation is a key pathogenic factor of COVID-19. Our research, along with others', has demonstrated that mast cells (MCs) play a vital role in the initiation of hyper-inflammation caused by SARS-CoV-2. In previous study, we observed that SARS-CoV-2 infection induced the accumulation of MCs in the peri-bronchus and bronchioalveolar-duct junction in humanized mice
-
Identification of fangchinoline as a broad-spectrum enterovirus inhibitor through reporter virus based high-content screening Virol. Sin. (IF 4.3) Pub Date : 2024-03-05 Qiu-Yan Zhang, Jia-Qi Li, Qi Li, Yang Zhang, Zhe-Rui Zhang, Xiao-Dan Li, Hong-Qing Zhang, Cheng-Lin Deng, Feng-Xia Yang, Yi Xu, Bo Zhang
Hand, foot, and mouth disease (HFMD) is a common pediatric illness mainly caused by enteroviruses, which are important human pathogens. Currently, there are no available antiviral agents for the therapy of enterovirus infection. In this study, an excellent high-content antiviral screening system utilizing the EV-A71-eGFP reporter virus was developed. Using this screening system, we screened a drug
-
Transmission restriction and genomic evolution co-shape the genetic diversity patterns of influenza A virus Virol. Sin. (IF 4.3) Pub Date : 2024-02-27 Xiao Ding, Jingze Liu, Taijiao Jiang, Aiping Wu
Influenza A virus (IAV) shows an extensive host range and rapid genomic variations, leading to continuous emergence of novel viruses with significant antigenic variations and the potential for cross-species transmission. This causes global pandemics and seasonal flu outbreaks, posing sustained threats worldwide. Thus, studying all IAVs' evolutionary patterns and underlying mechanisms is crucial for
-
Association analysis of genetic variants in critical patients with COVID-19 and validation in a Chinese population Virol. Sin. (IF 4.3) Pub Date : 2024-02-23 Yi Yin, Yi Zhang, Lelin Sun, Shuqiang Wang, Yong Zeng, Bo Gong, Lulin Huang, Yongquan He, Zhenglin Yang