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Unlocking the full potential of plant cell-based production for valuable proteins: Challenges and innovative strategies Biotechnol. Adv. (IF 12.1) Pub Date : 2025-02-04 Jianfeng Xu, Paula PerezSanchez, Shekoofeh Sadravi
Plant cell-based bioproduction systems offer a promising platform for the sustainable production of valuable proteins as they provide distinctive advantages over mammalian cell culture and whole plant cultivation. However, significant technical challenges remain, including low productivity, altered efficacy of plant-derived proteins, along with issues in culture process development, such as cell clumping
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Towards the application of nature's catalytic nanomachines: Cellulosomes in 2nd generation biofuel production Biotechnol. Adv. (IF 12.1) Pub Date : 2025-01-31 Maša Vodovnik, Nataša Lindič
Cellulosomes are highly efficient, complex multi-enzyme assemblies, predominantly found in anaerobic bacteria, which offer substantial potential for second-generation biofuel production through efficient lignocellulosic biomass degradation, thus reducing the need for costly pretreatments. Recent advances in cellulosome research have significantly contributed to developing more efficient consolidated
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Elucidation and biosynthesis of tetrahydroisoquinoline alkaloids: Recent advances and prospects Biotechnol. Adv. (IF 12.1) Pub Date : 2025-01-28 Yue Gao, Fei Li, Zhenbo Yuan, Zhengshan Luo, Yijian Rao
Tetrahydroisoquinoline alkaloids (THIAs) are a prominent class of plant-derived compounds with various important pharmaceutical applications. Considerable progress has been made in the biosynthesis of THIAs in microorganisms due to the elucidation of their natural biosynthetic pathways and the discovery of key enzymes. In this review, we systematically summarize recent progress in elucidating the natural
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Plant secondary metabolites against biotic stresses for sustainable crop protection Biotechnol. Adv. (IF 12.1) Pub Date : 2025-01-22 Tanzim Jahan, Md. Nurul Huda, Kaixuan Zhang, Yuqi He, Dili Lai, Namraj Dhami, Muriel Quinet, Md. Arfan Ali, Ivan Kreft, Sun-Hee Woo, Milen I. Georgiev, Alisdair R. Fernie, Meiliang Zhou
Sustainable agriculture practices are indispensable for achieving a hunger-free world, especially as the global population continues to expand. Biotic stresses, such as pathogens, insects, and pests, severely threaten global food security and crop productivity. Traditional chemical pesticides, while effective, can lead to environmental degradation and increase pest resistance over time. Plant-derived
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Electrode functional microorganisms in bioelectrochemical systems and its regulation: A review Biotechnol. Adv. (IF 12.1) Pub Date : 2025-01-13 Juping You, Lei Ye, Shihan Zhang, Jingkai Zhao, Yan Zhao, Yaxue He, Jianmeng Chen, Christian Kennes, Dongzhi Chen
Bioelectrochemical systems (BES) as environmental remediation biotechnologies have boomed in the last two decades. Although BESs combined technologies with electro-chemistry, −biology, and -physics, microorganisms and biofilms remain at their core. In this review, various functional microorganisms in BESs for CO2 reduction, dehalogenation, nitrate, phosphate, and sulfate reduction, metal removal, and
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Advancements of astaxanthin production in Haematococcus pluvialis: Update insight and way forward Biotechnol. Adv. (IF 12.1) Pub Date : 2025-01-10 Hongli Cui, Xiaoli Zhu, Xiao Yu, Siming Li, Kang Wang, Le Wei, Runzhi Li, Song Qin
The global market demand for natural astaxanthin (AXT) is growing rapidly owing to its potential human health benefits and diverse industry applications, driven by its safety, unique structure, and special function. Currently, the alga Haematococcus pluvialis (alternative name H. lacustris) has been considered as one of the best large-scale producers of natural AXT. However, the industry's further
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Advancement in synthetic gene circuits engineering: An alternative strategy for microRNA imaging and disease theranostics Biotechnol. Adv. (IF 12.1) Pub Date : 2025-01-09 Kulsoom, Wajahat Ali, Fu Wang
Gene circuits, which are genetically engineered systems designed to regulate gene expression, are emerging as powerful tools in disease theranostics, especially in mammalian cells. This review explores the latest advances in the design and application of gene circuits for detecting and treating various diseases. Synthetic gene circuits, inspired by electronic systems, offer precise control over therapeutic
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Recent advances in synthetic biology toolkits and metabolic engineering of Ralstonia eutropha H16 for production of value-added chemicals Biotechnol. Adv. (IF 12.1) Pub Date : 2025-01-09 Ye Wang, Yao Tian, Dake Xu, Shaoan Cheng, Wen-Wei Li, Hao Song
Ralstonia eutropha H16, a facultative chemolithoautotrophic Gram-negative bacterium, demonstrates remarkable metabolic flexibility by utilizing either diverse organic substrates or CO2 as the sole carbon source, with H2 serving as the electron donor under aerobic conditions. The capacity of carbon and energy metabolism of R. eutropha H16 enabled development of synthetic biology technologies and strategies
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Mycelium-based composites: An updated comprehensive overview Biotechnol. Adv. (IF 12.1) Pub Date : 2025-01-06 Emma Camilleri, Sumesh Narayan, Divnesh Lingam, Renald Blundell
Mycelium-based composites hold significant potential as sustainable alternatives to traditional materials, offering innovative solutions to the escalating challenges of global warming and climate change. This review examines their production techniques, advantages, and limitations, emphasizing their role in addressing pressing environmental and economic concerns. Current applications span various industries
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Chiral helical scaffolds: Unlocking their potential in biomolecular interactions and biomedical applications Biotechnol. Adv. (IF 12.1) Pub Date : 2025-01-03 Ghada Bouz, Jaroslav Žádný, Jan Storch, Jan Vacek
In nature, various molecules possess spiral geometry. Such helical structures are even prevalent within the human body, represented classically by DNA and three-dimensional (secondary structure) protein folding. In this review, we chose helicenes and helicene-like structures –synthetically accessible carbon-rich molecules– as a compelling example of helically chiral scaffolds. Helicene chemistry, traditionally
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Advances in bacterial glycoprotein engineering: A critical review of current technologies, emerging challenges, and future directions Biotechnol. Adv. (IF 12.1) Pub Date : 2025-01-02 Ziyu Li, Yujie Wang, Xiaojing Zhao, Qing Meng, Guozhen Ma, Lijie Xie, Xiaolong Jiang, Yutao Liu, Di Huang
Protein glycosylation, which involves the addition of carbohydrate chains to amino acid side chains, imparts essential properties to proteins, offering immense potential in synthetic biology applications. Despite its importance, natural glycosylation pathways present several limitations, highlighting the need for new tools to better understand glycan structures, recognition, metabolism, and biosynthesis
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Lignocellulosic biomass as promising substrate for polyhydroxyalkanoate production: Advances and perspectives Biotechnol. Adv. (IF 12.1) Pub Date : 2024-12-30 Dongna Li, Fei Wang, Xuening Zheng, Yingying Zheng, Xiaosen Pan, Jianing Li, Xiaojun Ma, Fen Yin, Qiang Wang
The depletion of fossil resources, coupled with global warming and adverse environmental impact of traditional petroleum-based plastics, have necessitated the discovery of renewable resources and innovative biodegradable materials. Lignocellulosic biomass (LB) emerges as a highly promising, sustainable and eco-friendly approach for accumulating polyhydroxyalkanoate (PHA), as it completely bypasses
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Biohydrogen fermentation from pretreated biomass in lignocellulose biorefinery: Effects of inhibitory byproducts and recent progress in mitigation strategies Biotechnol. Adv. (IF 12.1) Pub Date : 2024-12-29 Zi-Tong Zhao, Shan-Shan Yang, Geng Luo, Han-Jun Sun, Bing-Feng Liu, Guang-Li Cao, Mei-Yi Bao, Ji-Wei Pang, Nan-Qi Ren, Jie Ding
Lignocellulosic biomass (LCB) is expected to play a critical role in achieving the goal of biomass-to-bioenergy conversion because of its wide distribution and low price. Biomass fermentation is a promising method for the sustainable generation of biohydrogen (bioH2) from the renewable feedstock. Due to the inherent resistant structure of biomass, LCB needs to be pretreated to improve its digestibility
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Carboxysomes: The next frontier in biotechnology and sustainable solutions Biotechnol. Adv. (IF 12.1) Pub Date : 2024-12-26 Sulamita Santos Correa, Júnia Schultz, Brandon Zahodnik-Huntington, Andreas Naschberger, Alexandre Soares Rosado
Some bacteria possess microcompartments that function as protein-based organelles. Bacterial microcompartments (BMCs) sequester enzymes to optimize metabolic reactions. Several BMCs have been characterized to date, including carboxysomes and metabolosomes. Genomic analysis has identified novel BMCs and their loci, often including genes for signature enzymes critical to their function, but further characterization
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Biocatalytic oxyfunctionalization of unsaturated fatty acids to oxygenated chemicals via hydroxy fatty acids Biotechnol. Adv. (IF 12.1) Pub Date : 2024-12-26 Deok-Kun Oh, Tae-Eui Lee, Jin Lee, Kyung-Chul Shin, Jin-Byung Park
The selective oxyfunctionalization of unsaturated fatty acids is difficult in chemical reactions, whereas regio- and stereoselective oxyfunctionalization is often performed in biocatalytic synthesis. Fatty acid oxygenases, including hydratases, lipoxygenases, dioxygenases, diol synthases, cytochrome P450 monooxygenases, peroxygenases, and 12-hydroxylases, are used to convert C16 and C18 unsaturated
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Advanced technological approaches and market status analysis of xylose bioconversion and utilization: Xylooligosacharides and xylonic acid as emerging products Biotechnol. Adv. (IF 12.1) Pub Date : 2024-12-26 Jian Han, Faqiha Hamza, Jianming Guo, Mahmoud Sayed, Sang-Hyun Pyo, Yong Xu
The efficient conversion of xylose is a short board of cask effect to lignocellulosic biorefining, by markedly affecting the total economic and environmental benefits. Based on a comprehensive analysis of the current commercial status of traditional xylose utilization and industrial technology development, this review outlines new technological avenues for the efficient utilization of xylose from lignocellulosic
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HEK-omics: The promise of omics to optimize HEK293 for recombinant adeno-associated virus (rAAV) gene therapy manufacturing Biotechnol. Adv. (IF 12.1) Pub Date : 2024-12-19 Sai Guna Ranjan Gurazada, Hannah M. Kennedy, Richard D. Braatz, Steven J. Mehrman, Shawn W. Polson, Irene T. Rombel
Gene therapy is poised to transition from niche to mainstream medicine, with recombinant adeno-associated virus (rAAV) as the vector of choice. However, robust, scalable, industrialized production is required to meet demand and provide affordable patient access, which has not yet materialized. Closing the chasm between demand and supply requires innovation in biomanufacturing to achieve the essential
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Terahertz scanning near-field optical microscopy for biomedical detection: Recent advances, challenges, and future perspectives Biotechnol. Adv. (IF 12.1) Pub Date : 2024-12-19 Shihan Yan, Guanyin Cheng, Zhongbo Yang, Yuansen Guo, Ligang Chen, Ying Fu, Fucheng Qiu, Jonathan J. Wilksch, Tianwu Wang, Yiwen Sun, Junchao Fan, Xunbin Wei, Jiaguang Han, Fei Sun, Shixiang Xu, Huabin Wang
Terahertz (THz) radiation is widely recognized as a non-destructive, label-free, and highly- sensitive tool for biomedical detections. Nevertheless, its application in precision biomedical fields faces challenges due to poor spatial resolution caused by intrinsically long wavelength characteristics. THz scanning near-field optical microscopy (THz-SNOM), which surpasses the Rayleigh criterion, offers
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The role of the proteosurfaceome and exoproteome in bacterial coaggregation Biotechnol. Adv. (IF 12.1) Pub Date : 2024-12-16 Ana C. Afonso, Maria J. Saavedra, Manuel Simões, Lúcia C. Simões
Bacterial coaggregation is a critical process in multispecies biofilm formation, driven by specific molecular interactions that facilitate the adhesion and aggregation of bacterial cells. These interactions are essential for the development and persistence of complex microbial communities. This review provides a comprehensive analysis of the roles of the proteosurfaceome and exoproteome in bacterial
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From discovery to application: Enabling technology-based optimizing carbonyl reductases biocatalysis for active pharmaceutical ingredient synthesis Biotechnol. Adv. (IF 12.1) Pub Date : 2024-12-06 Jie Gu, Wanmeng Mu, Yan Xu, Yao Nie
The catalytic conversion of chiral alcohols and corresponding carbonyl compounds by carbonyl reductases (alcohol dehydrogenases), which are NAD(P) or NAD(P)H-dependent oxidoreductases, has attracted considerable attention. However, existing carbonyl reductases are insufficient to meet the demands of diverse industrial applications; hence, new enzymes with functions that can expand the toolbox of biocatalysts
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Direct cloning strategies for large genomic fragments: A review Biotechnol. Adv. (IF 12.1) Pub Date : 2024-12-06 Ya-Nan Chen, You-Zhi Cui, Xiang-Rong Chen, Jun-Yi Wang, Bing-Zhi Li, Ying-Jin Yuan
Mining large-scale functional regions of the genome helps to understand the essence of cellular life. The rapid accumulation of genomic information provides a wealth of material for genomic functional, evolutionary, and structural research. DNA cloning technology is an important tool for understanding, analyzing, and manipulating the genetic code of organisms. As synthetic biologists engineer greater
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Interpretable and explainable predictive machine learning models for data-driven protein engineering Biotechnol. Adv. (IF 12.1) Pub Date : 2024-12-05 David Medina-Ortiz, Ashkan Khalifeh, Hoda Anvari-Kazemabad, Mehdi D. Davari
Protein engineering through directed evolution and (semi)rational design has become a powerful approach for optimizing and enhancing proteins with desired properties. The integration of artificial intelligence methods has further accelerated protein engineering process by enabling the development of predictive models based on data-driven strategies. However, the lack of interpretability and transparency
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Is it possible to shape the microalgal biomass composition with operational parameters for target compound accumulation? Biotechnol. Adv. (IF 12.1) Pub Date : 2024-12-05 Ana F. Esteves, Ana L. Gonçalves, Vítor J.P. Vilar, José C.M. Pires
Microalgae, as photosynthetic microorganisms, offer a sustainable source of proteins, lipids, carbohydrates, pigments, vitamins, and antioxidants. Leveraging their advantages, such as fast growth, CO2 fixation, cultivation without arable land, and wastewater utilisation, microalgae can produce a diverse range of compounds. The extracted products find applications in bioenergy, animal feed, pharmaceuticals
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Engineering microbial cell factories by multiplexed spatiotemporal control of cellular metabolism: Advances, challenges, and future perspectives Biotechnol. Adv. (IF 12.1) Pub Date : 2024-12-05 Wenwen Yu, Ke Jin, Xianhao Xu, Yanfeng Liu, Jianghua Li, Guocheng Du, Jian Chen, Xueqin Lv, Long Liu
Generally, the metabolism in microbial organism is an intricate, spatiotemporal process that emerges from gene regulatory networks, which affects the efficiency of product biosynthesis. With the coming age of synthetic biology, spatiotemporal control systems have been explored as versatile strategies to promote product biosynthesis at both spatial and temporal levels. Meanwhile, the designer synthetic
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Current innovations in mRNA vaccines for targeting multidrug-resistant ESKAPE pathogens Biotechnol. Adv. (IF 12.1) Pub Date : 2024-12-03 Saber Imani, Shuojie Lv, Hongbo Qian, Yulan Cui, XiaoYan Li, Ali Babaeizad, Qingjing Wang
The prevalence of multidrug-resistant (MDR) ESKAPE pathogens, including Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, and Pseudomonas aeruginosa, represents a critical global public health challenge. In response, mRNA vaccines offer an adaptable and scalable platform for immunotherapy against ESKAPE pathogens by encoding specific antigens that stimulate
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Selection of aptamers targeting small molecules by capillary electrophoresis: Advances, challenges, and prospects Biotechnol. Adv. (IF 12.1) Pub Date : 2024-11-26 Yangyang Hu, Guangyu Jiang, Yalun Wen, Yuchen Shao, Ge Yang, Feng Qu
Aptamers, as novel recognition molecules, hold immense potential across various domains such as biosensing, nucleic acid drugs, medical diagnostics, as well as environmental and food analysis. The majority of aptamer selection processes targeting small molecules and protein commonly employ magnetic bead-based methodologies, wherein the target is initially immobilized on magnetic beads, followed by
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Expanding the frontiers of genome engineering: A comprehensive review of CRISPR-associated transposons Biotechnol. Adv. (IF 12.1) Pub Date : 2024-11-22 Cheng Chen, Ya-Wen Li, Yuan-Yuan Zheng, Xiu-Juan Li, Na Wu, Qi Guo, Tian-Qiong Shi, He Huang
Genome engineering is extensively utilized in diverse scientific disciplines, advancing human welfare and addressing various challenges. Numerous genome engineering tools have been developed to modify genomic sequences. Among these, the CRISPR-Cas system has transformed the field and remains the most commonly employed genome-editing tool. However, the CRISPR-Cas system relies on induced double-strand
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A non-classical view of antibody properties: Allosteric effect between variable and constant regions Biotechnol. Adv. (IF 12.1) Pub Date : 2024-11-21 Xiaoting Yu, Huiling Zhang, Tao Zhou, Kangliang Pan, Sayed Haidar Abbas Raza, Xing Shen, Hongtao Lei
Historically, antibodies have been divided into two functionally independent domains, the variable (V) region for antigen binding and the constant (C) region for mediating effector functions. However, this classical view of antibody function has been severely challenged by a large and growing number of studies, which reveal long-range conformational interactions and allosteric links between the V and
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Leveraging independent component analysis to unravel transcriptional regulatory networks: A critical review and future directions Biotechnol. Adv. (IF 12.1) Pub Date : 2024-11-20 Yuhan Zhang, Jianxiao Zhao, Xi Sun, Yangyang Zheng, Tao Chen, Zhiwen Wang
Transcriptional regulatory networks (TRNs) play a crucial role in exploring microbial life activities and complex regulatory mechanisms. The comprehensive reconstruction of TRNs requires the integration of large-scale experimental data, which poses significant challenges due to the complexity of regulatory relationships. The application of machine learning tools, such as clustering analysis, has been
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Advances and challenges in preparing membrane proteins for native mass spectrometry Biotechnol. Adv. (IF 12.1) Pub Date : 2024-11-20 Di Cheng, Yi Guo, Jixing Lyu, Yang Liu, Wenhao Xu, Weiyi Zheng, Yuchen Wang, Pei Qiao
Native mass spectrometry (nMS) is becoming a crucial tool for analyzing membrane proteins (MPs), yet challenges remain in solubilizing and stabilizing their native conformations while resolving and characterizing the heterogeneity introduced by post-translational modifications and ligand binding. This review highlights recent advancements and persistent challenges in preparing MPs for nMS. Optimizing
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Recent advances in culture medium design for enhanced production of monoclonal antibodies in CHO cells: A comparative study of machine learning and systems biology approaches Biotechnol. Adv. (IF 12.1) Pub Date : 2024-11-19 Hossein Kavoni, Iman Shahidi Pour Savizi, Nathan E. Lewis, Seyed Abbas Shojaosadati
The production of monoclonal antibodies (mAbs) using Chinese Hamster Ovary (CHO) cells has revolutionized the treatment of numerous diseases, solidifying their position as a cornerstone of the biopharmaceutical industry. However, achieving maximum mAb production while upholding strict product quality standards remains a significant hurdle. Optimizing cell culture media emerges as a critical factor
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Bacterial 5′ UTR: A treasure-trove for post-transcriptional regulation Biotechnol. Adv. (IF 12.1) Pub Date : 2024-11-17 Ya-Jun Liu, Xiaoqing Wang, Yuman Sun, Yingang Feng
In bacteria, where gene transcription and translation occur concurrently, post-transcriptional regulation is acknowledged to be effective and precise. The 5′ untranslated regions (5′ UTRs) typically harbor diverse post-transcriptional regulatory elements, like riboswitches, RNA thermometers, small RNAs, and upstream open reading frames, that serve to modulate transcription termination, translation
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Synthetic biology approaches to improve tolerance of inhibitors in lignocellulosic hydrolysates Biotechnol. Adv. (IF 12.1) Pub Date : 2024-11-16 Linyue Tian, Tianqi Qi, Fenghui Zhang, Vinh G. Tran, Jifeng Yuan, Yuanpeng Wang, Ning He, Mingfeng Cao
Increasing attention is being focused on using lignocellulose for valuable products. Microbial decomposition can convert lignocellulose into renewable biofuels and other high-value bioproducts, contributing to sustainable development. However, the presence of inhibitors in lignocellulosic hydrolysates can negatively affect microorganisms during fermentation. Improving microbial tolerance to these hydrolysates
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Fusarium as a promising fungal genus with potential application in bioremediation for pollutants mitigation: A review Biotechnol. Adv. (IF 12.1) Pub Date : 2024-11-12 Carmen Sánchez
Fusarium is genetically diverse and widely distributed geographically. It is one of the genera with more endophytes (which cause no damage to the host plants). This review highlights the capability of Fusarium species to degrade environmental pollutants and describes the biodegradation pathways of some of the emerging environmental contaminants. Some Fusarium species use metabolic strategies enabling
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Metabolic engineering of Corynebacterium glutamicum: Unlocking its potential as a key cell factory platform for organic acid production Biotechnol. Adv. (IF 12.1) Pub Date : 2024-11-07 Ming-Hou Li, Han Li, Xue Zhang, Yu-Chen Liang, Cheng Li, Meng-Lin Sun, Kai Li, Chen-Guang Liu, Anthony J. Sinskey
Corynebacterium glutamicum, a well-studied industrial model microorganism, has garnered widespread attention due to its ability for producing amino acids with a long history. In recent years, research efforts have been increasingly focused on exploring its potential for producing various organic acids beyond amino acids. Organic acids, which are characterized by their acidic functional groups, have
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Microbial electrosynthesis technology for CO2 mitigation, biomethane production, and ex-situ biogas upgrading Biotechnol. Adv. (IF 12.1) Pub Date : 2024-11-07 Tae Hyun Chung, Simran Kaur Dhillon, Chungheon Shin, Deepak Pant, Bipro Ranjan Dhar
Currently, global annual CO2 emissions from fossil fuel consumption are extremely high, surpassing tens of billions of tons, yet our capacity to capture and utilize CO2 remains below a small fraction of the amount generated. Microbial electrosynthesis (MES) systems, an integration of microbial metabolism with electrochemistry, have emerged as a highly efficient and promising bio-based carbon-capture-and-utilization
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Biotechnological applications of purine and pyrimidine deaminases Biotechnol. Adv. (IF 12.1) Pub Date : 2024-11-05 Jon Del Arco, Javier Acosta, Jesús Fernández-Lucas
Deaminases, ubiquitous enzymes found in all living organisms from bacteria to humans, serve diverse and crucial functions. Notably, purine and pyrimidine deaminases, while biologically essential for regulating nucleotide pools, exhibit exceptional versatility in biotechnology. This review systematically consolidates current knowledge on deaminases, showcasing their potential uses and relevance in the
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Harnessing Raman spectroscopy for cell therapy bioprocessing Biotechnol. Adv. (IF 12.1) Pub Date : 2024-10-28 Marta H.G. Costa, Inês Carrondo, Inês A. Isidro, Margarida Serra
Cell therapy manufacturing requires precise monitoring of critical parameters to ensure product quality, consistency and to facilitate the implementation of cost-effective processes. While conventional analytical methods offer limited real-time insights, integration of process analytical technology tools such as Raman spectroscopy in bioprocessing has the potential to drive efficiency and reliability
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Implications of glycosylation for the development of selected cytokines and their derivatives for medical use Biotechnol. Adv. (IF 12.1) Pub Date : 2024-10-22 Giulia Scapin, Ece Cagdas, Lise Marie Grav, Nathan E Lewis, Steffen Goletz, Lise Hafkenscheid
Cytokines are important regulators of immune responses, making them attractive targets for autoimmune diseases and cancer therapeutics. Yet, the significance of cytokine glycosylation remains underestimated. Many cytokines carry N- and O-glycans and some even undergo C-mannosylation. Recombinant cytokines produced in heterologous host cells may lack glycans or exhibit a different glycosylation pattern
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Constructed wetland microbial fuel cell as enhancing pollutants treatment technology to produce green energy Biotechnol. Adv. (IF 12.1) Pub Date : 2024-10-20 Iryna Rusyn, Julio César Gómora-Hernández
The persistent challenge of water pollution, exacerbated by slow progress in ecofriendly technologies and accumulating pollutants, underscores the need for innovative solutions. Constructed Wetland Microbial Fuel Cell (CW-MFC) emerges as an intriguing environmental technology capable of adressing this issue by eliminating contaminants from wastewater while simultaneously producing green energy as an
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Process simulation and evaluation of scaled-up biocatalytic systems: Advances, challenges, and future prospects Biotechnol. Adv. (IF 12.1) Pub Date : 2024-10-20 Zhonghao Chen, Lei Wang
With the increased demand for bio-based products and the rapid development of biomanufacturing technologies, biocatalytic reactions including microorganisms and enzyme based, have become promising approaches. Prior to the scale-up of production process, environmental and economic feasibility analysis are essential for the development of a sustainable and intelligent bioeconomy in the context of industry
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Exploring the versatility of Porphyridium sp.: A comprehensive review of cultivation, bio-product extraction, purification, and characterization techniques Biotechnol. Adv. (IF 12.1) Pub Date : 2024-10-20 Anh Quynh Nguyen, Maedeh Mohammadi, Mahsa Alian, Gangatharan Muralitharan, Vikas Singh Chauhan, Venkatesh Balan
Interest in red microalgae of the Porphyridium genus has surged due to their richness in phycobiliproteins, polyunsaturated fatty acids, and sulfated polysaccharides. These biomasses and their derivatives find applications across food, feed, nutraceutical, pharmaceutical, and cosmetic industries. A deeper understanding of their properties and extraction methods is essential to optimize downstream processing
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Utilizing stimuli-responsive nanoparticles to deliver and enhance the anti-tumor effects of bilirubin Biotechnol. Adv. (IF 12.1) Pub Date : 2024-10-18 Elaheh Mirhadi, Alexandra E. Butler, Prashant Kesharwani, Amirhossein Sahebkar
Bilirubin (BR) is among the most potent endogenous antioxidants that originates from the heme catabolic pathway. Despite being considered as a dangerous and cytotoxic waste product at high concentrations, BR has potent antioxidant effects leading to the reduction of oxidative stress and inflammation, which play an important role in the development and progression of cancer. The purpose of this study
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Exploring the landscape of FRET-based molecular sensors: Design strategies and recent advances in emerging applications Biotechnol. Adv. (IF 12.1) Pub Date : 2024-10-16 Neha Soleja, Mohd. Mohsin
Probing biological processes in living organisms that could provide one-of-a-kind insights into real-time alterations of significant physiological parameters is a formidable task that calls for specialized analytic devices. Classical biochemical methods have significantly aided our understanding of the mechanisms that regulate essential biological processes. These methods, however, are typically insufficient
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An overview of biomethanation and the use of membrane technologies as a candidate to overcome H2 mass transfer limitations Biotechnol. Adv. (IF 12.1) Pub Date : 2024-10-14 Manuel Fachal-Suárez, Santhana Krishnan, Sumate Chaiprapat, Daniel González, David Gabriel
Energy produced from renewable sources such as sun or wind are intermittent, depending on circumstantial factors. This fact explains why energy supply and demand do not match. In this context, the interest in biomethanation has increased as an interesting contribution to the Power-to-gas concept (P2G), transforming the extra amount of produced electricity into methane (CH4). The reaction between green
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The current progress of tandem chemical and biological plastic upcycling Biotechnol. Adv. (IF 12.1) Pub Date : 2024-10-10 Yifeng Hu, Yuxin Tian, Chenghao Zou, Tae Seok Moon
Each year, millions of tons of plastics are produced for use in such applications as packaging, construction, and textiles. While plastic is undeniably useful and convenient, its environmental fate and transport have raised growing concerns about waste and pollution. However, the ease and low cost of producing virgin plastic have so far made conventional plastic recycling economically unattractive
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The new chassis in the flask: Advances in Vibrio natriegens biotechnology research Biotechnol. Adv. (IF 12.1) Pub Date : 2024-10-09 Matthew Lima, Charandatta Muddana, Zhengyang Xiao, Anindita Bandyopadhyay, Pramod P. Wangikar, Himadri B. Pakrasi, Yinjie J. Tang
Biotechnology has been built on the foundation of a small handful of well characterized and well-engineered organisms. Recent years have seen a breakout performer gain attention as a new entrant into the bioengineering toolbox: Vibrio natriegens. This review covers recent research efforts into making V. natriegens a biotechnology platform, using a large language model (LLM) and knowledge graph to expedite
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From lignocellulosic biomass to single cell oil for sustainable biomanufacturing: Current advances and prospects Biotechnol. Adv. (IF 12.1) Pub Date : 2024-10-09 Yu Duan, Limei Chen, Longxue Ma, Farrukh Raza Amin, Yida Zhai, Guofu Chen, Demao Li
As global temperatures rise and arid climates intensify, the reserves of Earth's resources and the future development of humankind are under unprecedented pressure. Traditional methods of food production are increasingly inadequate in meeting the demands of human life while remaining environmentally sustainable and resource-efficient. Consequently, the sustainable supply of lipids is expected to become
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Engineered transcription factor-binding diversed functional nucleic acid-based synthetic biosensor Biotechnol. Adv. (IF 12.1) Pub Date : 2024-10-05 Yanger Liu, Ziying Zhou, Yifan Wu, Lei Wang, Jiageng Cheng, Longjiao Zhu, Yulan Dong, Jie Zheng, Wentao Xu
Engineered transcription factors (eTFs) binding diversed functional nucleic acids (dFNAs), as innovative biorecognition systems, have gradually become indispensable core elements for building synthetic biosensors. They not only circumvent the limitations of the original TF-based biosensing technologies, but also inject new vitality into the field of synthetic biosensing. This review aims to provide
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Design and application of microfluidics in aptamer SELEX and Aptasensors Biotechnol. Adv. (IF 12.1) Pub Date : 2024-10-05 Shikun Zhang, Yingming Zhang, Zhiyuan Ning, Mengxia Duan, Xianfeng Lin, Nuo Duan, Zhouping Wang, Shijia Wu
Aptamers are excellent recognition molecules obtained from systematic evolution of ligands by exponential enrichment (SELEX) that have been extensively researched for constructing aptasensors. However, in the process from SELEX to the construction of aptasensors, there are many disadvantages, such as tedious and repetitive operations, interference from external factors, and low efficiency, which seriously
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Protein representations: Encoding biological information for machine learning in biocatalysis Biotechnol. Adv. (IF 12.1) Pub Date : 2024-10-02 David Harding-Larsen, Jonathan Funk, Niklas Gesmar Madsen, Hani Gharabli, Carlos G. Acevedo-Rocha, Stanislav Mazurenko, Ditte Hededam Welner
Enzymes offer a more environmentally friendly and low-impact solution to conventional chemistry, but they often require additional engineering for their application in industrial settings, an endeavour that is challenging and laborious. To address this issue, the power of machine learning can be harnessed to produce predictive models that enable the in silico study and engineering of improved enzymatic
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Microbiome regulation for sustainable wastewater treatment Biotechnol. Adv. (IF 12.1) Pub Date : 2024-09-27 Ke Shi, Jia-Min Xu, Han-Lin Cui, Hao-Yi Cheng, Bin Liang, Ai-Jie Wang
Sustainable wastewater treatment is essential for attaining clean water and sanitation, aligning with UN Sustainable Development Goals. Wastewater treatment plants (WWTPs) have utilized environmental microbiomes in biological treatment processes in this effort for over a century. However, the inherent complexity and redundancy of microbial communities, and emerging chemical and biological contaminants
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Engineering conditional protein-protein interactions for dynamic cellular control Biotechnol. Adv. (IF 12.1) Pub Date : 2024-09-27 Anthony M. Stohr, Derron Ma, Wilfred Chen, Mark Blenner
Conditional protein-protein interactions enable dynamic regulation of cellular activity and are an attractive approach to probe native protein interactions, improve metabolic engineering of microbial factories, and develop smart therapeutics. Conditional protein-protein interactions have been engineered to respond to various chemical, light, and nucleic acid-based stimuli. These interactions have been
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Bioengineered heparin: Advances in production technology Biotechnol. Adv. (IF 12.1) Pub Date : 2024-09-24 Razia Sultana, Masamichi Kamihira
Heparin, a highly sulfated glycosaminoglycan, is considered an indispensable anticoagulant with diverse therapeutic applications and has been a mainstay in medical practice for nearly a century. Its potential extends beyond anticoagulation, showing promise in treating inflammation, cancer, and infectious diseases such as COVID-19. However, its current sourcing from animal tissues poses challenges due
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Advances in biosynthesis and downstream processing of diols Biotechnol. Adv. (IF 12.1) Pub Date : 2024-09-19 Yongfei Liu, Chijian Zhang, An-Ping Zeng
Diols are important platform chemicals with a wide range of applications in the fields of chemical and pharmaceutical industries, food, feed and cosmetics. In particular, 1,3-propanediol (PDO), 1,4-butanediol (1,4-BDO) and 1,3-butanediol (1,3-BDO) are appealing monomers for producing industrially important polymers and plastics. Therefore, the commercialization of bio-based diols is highly important
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Advances in multi-enzyme co-localization strategies for the construction of microbial cell factory Biotechnol. Adv. (IF 12.1) Pub Date : 2024-09-13 Qi Guo, Yu-Xin Yang, Dong-Xun Li, Xiao-Jun Ji, Na Wu, Yue-Tong Wang, Chao Ye, Tian-Qiong Shi
Biomanufacturing, driven by technologies such as synthetic biology, offers significant potential to advance the bioeconomy and promote sustainable development. It is anticipated to transform traditional manufacturing and become a key industry in future strategies. Cell factories are the core of biomanufacturing. The advancement of synthetic biology and growing market demand have led to the production
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Droplet-based single-cell sequencing: Strategies and applications Biotechnol. Adv. (IF 12.1) Pub Date : 2024-09-11 Yuting Shang, Zhengzheng Wang, Liqing Xi, Yantao Wang, Meijing Liu, Ying Feng, Juan Wang, Qingping Wu, Xinran Xiang, Moutong Chen, Yu Ding
Notable advancements in single-cell omics technologies have not only addressed longstanding challenges but also enabled unprecedented studies of cellular heterogeneity with unprecedented resolution and scale. These strides have led to groundbreaking insights into complex biological systems, paving the way for a more profound comprehension of human biology and diseases. The droplet microfluidic technology
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Recent advances in engineering synthetic biomolecular condensates Biotechnol. Adv. (IF 12.1) Pub Date : 2024-09-11 Li Wan, Juntao Ke, Yingying Zhu, Wenli Zhang, Wanmeng Mu
Biomolecular condensates are intriguing entities found within living cells. These structures possess the ability to selectively concentrate specific components through phase separation, thereby playing a crucial role in the spatiotemporal regulation of a wide range of cellular processes and metabolic activities. To date, extensive studies have been dedicated to unraveling the intricate connections
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The biosynthesis of L-phenylalanine-derived compounds by engineered microbes Biotechnol. Adv. (IF 12.1) Pub Date : 2024-09-11 Min Qiu, Jie Jiang, Wankui Jiang, Wenming Zhang, Yujia Jiang, Fengxue Xin, Min Jiang
L-Phenylalanine (L-Phe) is an important aromatic amino acid, which has been widely used in food, health care products, medicine and other fields. Based on the relatively mature microbial biosynthesis process, a variety of L-phenylalanine-derived compounds have attracted more and more attentions owing to their extensively potential applications in the fields of food, medicine, spices, cosmetics, and