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Engineering from evolution Nat. Chem. Biol. (IF 14.8) Pub Date : 2024-04-26 Francesco Zamberlan
Polyketides are a group of natural products with valuable pharmacological activities, biosynthesized by bacterial or fungal megaenzymes called polyketide synthases (PKSs). These are composed of multiple modules in an assembly line, each introducing a specific moiety of the final compound in a stepwise manner. Among them, trans-acyltransferase PKSs have evolved through the recombination of biosynthetic
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Bacteria lose a pep in their step Nat. Chem. Biol. (IF 14.8) Pub Date : 2024-04-26 Gene Chong
Zhang et al. hypothesized that a structurally similar disaccharide, GlcNAc-1,6-anhydro-MurNAc, could be a minimal moiety that acts as a competitive inhibitor to lipid II and lacks a reducing end for glycan chain elongation. The authors developed a 15-step total synthesis of the disaccharide-pentapeptide unit, called compound 1, and the disaccharide-only compound, 1-deAA. They incubated compound 1 with
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Setting the pace for degrons Nat. Chem. Biol. (IF 14.8) Pub Date : 2024-04-26 Yiyun Song
Immunomodulatory drugs (IMiDs), including thalidomide, pomalidomide and lenalidomide, act as molecular glues to induce the ubiquitination and degradation of their target proteins (referred to as neosubstrates) by facilitating their direct interaction with cereblon (CRBN), the substrate receptor of the CRL4CRBN E3 ubiquitin ligase. Some neosubstrates contain a C2H2 zinc finger motif that is responsible
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Chemical screening by time-resolved X-ray scattering to discover allosteric probes Nat. Chem. Biol. (IF 14.8) Pub Date : 2024-04-26 Chris A. Brosey, Todd M. Link, Runze Shen, Davide Moiani, Kathryn Burnett, Greg L. Hura, Darin E. Jones, John A. Tainer
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Analysis and benchmarking of small and large genomic variants across tandem repeats Nat. Biotechnol. (IF 46.9) Pub Date : 2024-04-26 Adam C. English, Egor Dolzhenko, Helyaneh Ziaei Jam, Sean K. McKenzie, Nathan D. Olson, Wouter De Coster, Jonghun Park, Bida Gu, Justin Wagner, Michael A. Eberle, Melissa Gymrek, Mark J. P. Chaisson, Justin M. Zook, Fritz J. Sedlazeck
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Current advances in photocatalytic proximity labeling Cell Chem. Bio. (IF 8.6) Pub Date : 2024-04-24 Steve D. Knutson, Benito F. Buksh, Sean W. Huth, Danielle C. Morgan, David W.C. MacMillan
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Pyro(phospho)mania Nat. Chem. Biol. (IF 14.8) Pub Date : 2024-04-25 Claire E. Eyers, Christopher J. Clarke
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Minimizing higher-order aggregation maximizes iron mobilization by small molecules Nat. Chem. Biol. (IF 14.8) Pub Date : 2024-04-25 Andrew D. Blake, Jianhua Chao, Anna M. SantaMaria, Stella Ekaputri, Kelsie J. Green, Samantha T. Brown, Christopher K. Rakowski, Eun-Kyung Choi, Luisa Aring, Peng-Jui Chen, Nicholas M. Snead, Douglas M. Matje, Tao Geng, Angela Octaviani, Keith Bailey, Stanley J. Hollenbach, Timothy M. Fan, Young-Ah Seo, Martin D. Burke
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Extensive protein pyrophosphorylation revealed in human cell lines Nat. Chem. Biol. (IF 14.8) Pub Date : 2024-04-25 Jeremy A. M. Morgan, Arpita Singh, Leonie Kurz, Michal Nadler-Holly, Max Ruwolt, Shubhra Ganguli, Sheenam Sharma, Martin Penkert, Eberhard Krause, Fan Liu, Rashna Bhandari, Dorothea Fiedler
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Expression of neuronal NO synthase α- and β-isoforms in skeletal muscle of mice Biochem. J. (IF 4.1) Pub Date : 2024-05-08 Baum, Oliver
Knowledge of the primary structure of neuronal NO synthase (nNOS) in skeletal muscle is still conflicting and needs further clarification. To elucidate the expression patterns of nNOS isoforms at both mRNA and protein level, systematic reverse transcription (RT)-PCR and epitope mapping by qualitative immunoblot analysis on skeletal muscle of C57/BL6 mice were performed. The ability of the nNOS isoforms
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Structural basis for α-tubulin-specific and modification state-dependent glutamylation Nat. Chem. Biol. (IF 14.8) Pub Date : 2024-04-24 Kishore K. Mahalingan, Danielle A. Grotjahn, Yan Li, Gabriel C. Lander, Elena A. Zehr, Antonina Roll-Mecak
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Molecular recording of calcium signals via calcium-dependent proximity labeling Nat. Chem. Biol. (IF 14.8) Pub Date : 2024-04-24 J. Wren Kim, Adeline J. H. Yong, Erin E. Aisenberg, Joseph H. Lobel, Wei Wang, Ted M. Dawson, Valina L. Dawson, Ruixuan Gao, Yuh Nung Jan, Helen S. Bateup, Nicholas T. Ingolia
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Repurposing AS1411 for constructing ANM-PROTACs Cell Chem. Bio. (IF 8.6) Pub Date : 2024-04-23 Xuekun Fu, Jin Li, Xinxin Chen, Hongzhen Chen, Zhuqian Wang, Fang Qiu, Duoli Xie, Jie Huang, Siran Yue, Chunhao Cao, Yiying Liang, Aiping Lu, Chao Liang
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Addendum: Designing microbial consortia with defined social interactions Nat. Chem. Biol. (IF 14.8) Pub Date : 2024-04-22 Wentao Kong, James J. Collins, Ting Lu
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Assessing the laboratory performance of AI-generated enzymes Nat. Biotechnol. (IF 46.9) Pub Date : 2024-04-23
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Discovery of tumor-reactive T cell receptors by massively parallel library synthesis and screening Nat. Biotechnol. (IF 46.9) Pub Date : 2024-04-23 Ziva Moravec, Yue Zhao, Rhianne Voogd, Danielle R. Cook, Seon Kinrot, Benjamin Capra, Haiyan Yang, Brenda Raud, Jiayu Ou, Jiekun Xuan, Teng Wei, Lili Ren, Dandan Hu, Jun Wang, John B.A.G. Haanen, Ton N. Schumacher, Xi Chen, Ely Porter, Wouter Scheper
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Therapeutic application of circular RNA aptamers in a mouse model of psoriasis Nat. Biotechnol. (IF 46.9) Pub Date : 2024-04-23 Si-Kun Guo, Chu-Xiao Liu, Yi-Feng Xu, Xiao Wang, Fang Nan, Youkui Huang, Siqi Li, Shan Nan, Ling Li, Edo Kon, Chen Li, Meng-Yuan Wei, Rina Su, Jia Wei, Shiguang Peng, Nitay Ad-El, Jiaquan Liu, Dan Peer, Ting Chen, Li Yang, Ling-Ling Chen
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Computational scoring and experimental evaluation of enzymes generated by neural networks Nat. Biotechnol. (IF 46.9) Pub Date : 2024-04-23 Sean R. Johnson, Xiaozhi Fu, Sandra Viknander, Clara Goldin, Sarah Monaco, Aleksej Zelezniak, Kevin K. Yang
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Facile generation of biepitopic antibodies with intrinsic agonism for activating tumor necrosis factor receptors Cell Chem. Bio. (IF 8.6) Pub Date : 2024-04-22 Harkamal S. Jhajj, John S. Schardt, Namir Khalasawi, Emily L. Yao, Timon S. Lwo, Na-Young Kwon, Ryen L. O’Meara, Alec A. Desai, Peter M. Tessier
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UFL1 triggers replication fork degradation by MRE11 in BRCA1/2-deficient cells Nat. Chem. Biol. (IF 14.8) Pub Date : 2024-04-22 Tian Tian, Junliang Chen, Huacun Zhao, Yulin Li, Feiyu Xia, Jun Huang, Jinhua Han, Ting Liu
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Why Japan lacks a vibrant biotech industry Nat. Biotechnol. (IF 46.9) Pub Date : 2024-04-22 Mark Kessel, Chris Vickrey
Recent analysis of the biopharmaceutical industry in Japan has emphasized that the lack of a thriving biotech ecosystem in that country is largely due to tight controls on drug pricing1. However, this is only one part of the explanation, and any strategy to promote Japanese biotech must acknowledge the full complexity of the problem. Japan has long punched above its weight in innovative research in
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Biochemical and structural impact of two novel missense mutations in cystathionine β-synthase gene associated with homocystinuria Biochem. J. (IF 4.1) Pub Date : 2024-04-24 Al-Sadeq, Duaa W., Conter, Carolina, Thanassoulas, Angelos, Al-Dewik, Nader, Safieh-Garabedian, Bared, Martínez-Cruz, Luis Alfonso, Nasrallah, Gheyath K., Astegno, Alessandra, Nomikos, Michail
Homocystinuria is a rare disease caused by mutations in the CBS gene that results in a deficiency of cystathionine β-synthase (CBS). CBS is an essential pyridoxal 5′-phosphate (PLP)-dependent enzyme in the transsulfuration pathway, responsible for combining serine with homocysteine to produce cystathionine, whose activity is enhanced by the allosteric regulator S-adenosylmethionine (SAM). CBS also
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AMP-activated protein kinase can be allosterically activated by ADP but AMP remains the key activating ligand Biochem. J. (IF 4.1) Pub Date : 2024-04-24 Hawley, Simon A., Russell, Fiona M., Hardie, D. Grahame
The AMP-activated protein kinase (AMPK) is a sensor of cellular energy status. When activated by increases in ADP:ATP and/or AMP:ATP ratios (signalling energy deficit), AMPK acts to restore energy balance. Binding of AMP to one or more of three CBS repeats (CBS1, CBS3, CBS4) on the AMPK-γ subunit activates the kinase complex by three complementary mechanisms: (i) promoting α-subunit Thr172 phosphorylation
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Strategies of bacterial detection by inflammasomes Cell Chem. Bio. (IF 8.6) Pub Date : 2024-04-17 Jordan B. Jastrab, Jonathan C. Kagan
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Meet the authors: Lori Emert-Sedlak and Tom Smithgall Cell Chem. Bio. (IF 8.6) Pub Date : 2024-04-18 Lori Emert-Sedlak, Tom Smithgall
In an interview with Samantha Nelson, a scientific editor of Cell Chemical Biology, the first and corresponding authors of the research article entitled “PROTAC-mediated degradation of HIV-1 Nef efficiently restores cell-surface CD4 and MHC-I expression and blocks HIV-1 replication” share insights on their paper and life as scientists.
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Meet the Authors: Christopher R. Mansfield and Emily R. Derbyshire Cell Chem. Bio. (IF 8.6) Pub Date : 2024-04-18 Christopher R. Mansfield, Emily R. Derbyshire
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Dual-action compounds unleash a one-two punch against tuberculosis Cell Chem. Bio. (IF 8.6) Pub Date : 2024-04-18 Wendy Le Mouëllic, Yannick Poquet, Olivier Neyrolles
In this issue of Cell Chemical Biology, Gries et al.1 employ an innovative screening approach to identify anti-tuberculosis compounds with dual modes of action: anti-virulence against the type VII secretion system ESX-1 and enhanced ethionamide efficacy. These compounds hold promise for developing multi-target tuberculosis drugs with potential clinical applications.
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METTL3: Melting the tumor microenvironment for improved immunotherapy Cell Chem. Bio. (IF 8.6) Pub Date : 2024-04-18 Longfei Gao, Lei Ding
The tumor microenvironment (TME) dictates the outcome of cancer immunotherapy. In this issue of Cell Chemical Biology, Yu et al.1 report that targeting Mettl3 leads to a more inflamed, “hot” TME and effective anti-PD-1 therapy. This study points to a new target in remodeling the TME for improved immunotherapy.
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SARS-CoV-2 resistance to monoclonal antibodies and small-molecule drugs Cell Chem. Bio. (IF 8.6) Pub Date : 2024-04-18 Sho Iketani, David D. Ho
Over four years have passed since the beginning of the COVID-19 pandemic. The scientific response has been rapid and effective, with many therapeutic monoclonal antibodies and small molecules developed for clinical use. However, given the ability for viruses to become resistant to antivirals, it is perhaps no surprise that the field has identified resistance to nearly all of these compounds. Here,
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A peptide dehydratase with core strength Nat. Chem. Biol. (IF 14.8) Pub Date : 2024-04-19 Daniel Richter, Anna Lisa Vagstad
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Forced rewiring of RTK signaling Nat. Chem. Biol. (IF 14.8) Pub Date : 2024-04-18 Ahsan Ausaf Ali, Mahmoud Amouzadeh Tabrizi, Mingxu You
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The Multifaceted Role of Intracellular Glycosylation in Cytoprotection and Heart Disease J. Biol. Chem. (IF 5.5) Pub Date : 2024-04-18 Priya Umapathi, Akanksha Aggarwal, Fiddia Zahra, Bhargavi Narayanan, Natasha E. Zachara
The modification of nuclear, cytoplasmic, and mitochondrial proteins by O-linked β-N-acetylglucosamine (O-GlcNAc) is an essential post-translational modification common in metazoans. O-GlcNAc is cycled on and off proteins in response to environmental and physiological stimuli impacting protein function, which, in turn, tunes pathways that include transcription, translation, proteostasis, signal transduction
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Discovery of potent inhibitors of α-synuclein aggregation using structure-based iterative learning Nat. Chem. Biol. (IF 14.8) Pub Date : 2024-04-17 Robert I. Horne, Ewa A. Andrzejewska, Parvez Alam, Z. Faidon Brotzakis, Ankit Srivastava, Alice Aubert, Magdalena Nowinska, Rebecca C. Gregory, Roxine Staats, Andrea Possenti, Sean Chia, Pietro Sormanni, Bernardino Ghetti, Byron Caughey, Tuomas P. J. Knowles, Michele Vendruscolo
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Customized molecular glue complexes with desired properties Nat. Biotechnol. (IF 46.9) Pub Date : 2024-04-17 Iris Marchal
Degron tags can trigger rapid and temporally controlled degradation of proteins using small molecules known as molecular glues. These molecular glues induce or stabilize protein interactions between a target protein and a ubiquitin ligase. As research tools, molecular glue complexes are limited by the large size of degron tags, which prevents integration in endogenous protein-coding genes. In a study
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World’s priciest drug treats MLD Nat. Biotechnol. (IF 46.9) Pub Date : 2024-04-17
The US Food and Drug Administration has approved the first therapy for metachromatic leukodystrophy (MLD), a rare fatal genetic disorder. The lysosomal storage disease affects about 40 children each year in the USA. It is caused by a mutation in the gene encoding the arylsulfatase enzyme that leads to progressive demyelination and progressive loss of motor and cognitive functions. There were previously
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Saving Cavendish Nat. Biotechnol. (IF 46.9) Pub Date : 2024-04-17
Farmers have a green light to grow the first genetically modified banana. The wilt-proof strain of the Cavendish banana developed by researchers from the Queensland University of Technology is resistant to Panama disease (Fusarium wilt), a devastating fungus. The Office of the Gene Technology Regulator in Australia gave the go-ahead on 12 February to allow the genetically modified banana to be grown
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Prime editing Nat. Biotechnol. (IF 46.9) Pub Date : 2024-04-17
Recent patents relating to methods and compositions for prime editing.
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FDA approves first MASH drug Nat. Biotechnol. (IF 46.9) Pub Date : 2024-04-17
The first drug to treat fatty liver disease due to metabolic dysfunction-associated steatohepatitis (MASH) has been given a green light by the US Food and Drug Administration. Madrigal Pharmaceuticals’ Rezdiffra (resmetirom) received an accelerated approval to treat the disease, previously known as non-alcoholic steatohepatitis (NASH). In this progressive liver condition fat buildup triggers inflammation
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Biotech news from around the world Nat. Biotechnol. (IF 46.9) Pub Date : 2024-04-17
The Ministry of Health and Welfare selects Johnson & Johnson’s JLABS to operate the country’s global accelerator platform. JLABS will engage with various local incubators and collaborators in the startup ecosystem to offer venture development programs, stimulate employment and encourage commercialization to enhance the global competitiveness of Korea’s life sciences sector. Costa Rica revises its biotech
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First gene-edited pig kidney transplant Nat. Biotechnol. (IF 46.9) Pub Date : 2024-04-17
Surgeons at Massachusetts General Hospital have transplanted a pig kidney into a living person for the first time. On 16 March, a 62-year old man with end-stage kidney disease received a kidney from a genome-edited pig developed by eGenesis. The humanized pig organ was taken from a genetically engineered Yucatan miniature pig carrying a total of 69 gene edits designed to increase compatibility between
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RoseTTAFold expands to all-atom for biomolecular prediction and design Nat. Biotechnol. (IF 46.9) Pub Date : 2024-04-17 Iris Marchal
Deep learning methods enable the structural prediction of proteins with high accuracy but are unable to model non-protein molecules that are essential for a protein’s biological function. Writing in Science, Krishna et al. introduce RoseTTAFold All-Atom (RFAA) to model the structure of full biological assemblies containing proteins, nucleic acids, small molecules, metals and covalent modifications
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Mapping the landscape of host–microbiome interactions Nat. Biotechnol. (IF 46.9) Pub Date : 2024-04-17 Iris Marchal
The human body hosts countless microorganisms that play diverse roles in health and disease. However, the lack of tools capable of investigating these host–microbiota interactions at large scale has left many of them undiscovered. Writing in Nature, Sonnert et al. developed and validated a tool, named BASEHIT, to map the broad interplay between bacteria and human proteins. BASEHIT identified an extensive
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Prime editing deal flurry to nail down patent rights Nat. Biotechnol. (IF 46.9) Pub Date : 2024-04-17
The prime editing field is booming, with companies making strategic decisions to avoid an IP showdown.
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Startups probe hidden viruses in the ‘dark genome’ to treat disease Nat. Biotechnol. (IF 46.9) Pub Date : 2024-04-17
Drug hunters are finding that ancient virus-like artifacts in the human genome could offer new avenues to treat neurodegeneration, cancer, autoimmunity and even aging with antibodies, vaccines and antiretroviral agents.
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Video game unleashes millions of citizen scientists on microbiome research Nat. Biotechnol. (IF 46.9) Pub Date : 2024-04-17
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Five questions with César de la Fuente Nat. Biotechnol. (IF 46.9) Pub Date : 2024-04-17 Michael Francisco
A pioneer in the emerging fields of AI for antibiotic discovery and molecular de-extinction describes his transdisciplinary background and lifelong passion to understand biology.
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People Nat. Biotechnol. (IF 46.9) Pub Date : 2024-04-17
Recent moves of note in and around the biotech and pharma industries.
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Mapping the global landscape for induced pluripotent stem cells from patents and clinical trials Nat. Biotechnol. (IF 46.9) Pub Date : 2024-04-17 Liyang Lyu, Ye Feng, Borong Huang, Ren-He Xu, Yuanjia Hu
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Sharing best practices for educational programs on venture creation and commercialization Nat. Biotechnol. (IF 46.9) Pub Date : 2024-04-17 Jordan Eidlisz, Zachary Hill-Whilton, Gabriel Vizgan, Daniel Cobos, Sadhana Chitale, Colleen Gillespie, Nabil Dib, Gabrielle Gold-von Simson
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Improving microbial phylogeny with citizen science within a mass-market video game Nat. Biotechnol. (IF 46.9) Pub Date : 2024-04-15 Roman Sarrazin-Gendron, Parham Ghasemloo Gheidari, Alexander Butyaev, Timothy Keding, Eddie Cai, Jiayue Zheng, Renata Mutalova, Julien Mounthanyvong, Yuxue Zhu, Elena Nazarova, Chrisostomos Drogaris, Kornél Erhart, Amélie Brouillette, Gabriel Richard, Randy Pitchford, Sébastien Caisse, Mathieu Blanchette, Daniel McDonald, Rob Knight, Attila Szantner, Jérôme Waldispühl
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Bringing chemistry to medicine to redefine the undruggable Nat. Chem. Biol. (IF 14.8) Pub Date : 2024-04-12 Caitlin D. Deane, Marcus Fischer, Anang A. Shelat
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Inferring gene regulatory networks from single-cell multiome data using atlas-scale external data Nat. Biotechnol. (IF 46.9) Pub Date : 2024-04-12 Qiuyue Yuan, Zhana Duren
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Activin E is a transforming growth factor β ligand that signals specifically through activin receptor-like kinase 7 Biochem. J. (IF 4.1) Pub Date : 2024-04-10 Vestal, Kylie A., Kattamuri, Chandramohan, Koyiloth, Muhasin, Ongaro, Luisina, Howard, James A., Deaton, Aimee M., Ticau, Simina, Dubey, Aditi, Bernard, Daniel J., Thompson, Thomas B.
Activins are one of the three distinct subclasses within the greater Transforming growth factor β (TGFβ) superfamily. First discovered for their critical roles in reproductive biology, activins have since been shown to alter cellular differentiation and proliferation. At present, members of the activin subclass include activin A (ActA), ActB, ActC, ActE, and the more distant members myostatin and GDF11
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Correction: Proteasome and thiol involvement in quality control of glycosylphosphatidylinositol anchor addition Biochem. J. (IF 4.1) Pub Date : 2024-04-10 Wilbourn, Barry, Nesbeth, Darren N., Wainwright, Linda J., Field, Mark C.
The authors of the original article “Proteasome and thiol involvement in quality control of glycosylphosphatidylinositol anchor addition” DOI: 10.1042/bj3320111: Wilbourn et al., Biochem. J.332, 111–118 (1998) would like to correct Figure 5 of this article. After publication, a reader identified that Figure 5 contained a duplicated Western blot image in panel ‘B’ between the “28” and “29” experimental
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Expression of Concern: Protease-activated receptor-2 promotes kidney tubular epithelial inflammation by inhibiting autophagy via the PI3K/Akt/mTOR signalling pathway Biochem. J. (IF 4.1) Pub Date : 2024-04-10 Du, Chunyang, Zhang, Tao, Xiao, Xia, Shi, Yonghong, Duan, Huijun, Ren, Yunzhuo
The Editorial Office has been made aware of potential issues surrounding the scientific validity of this paper, hence has issued an expression of concern to notify readers whilst the Editorial Office investigates. It has been noted that there seems to be a partial duplication between Figure 4C PAR2-OE control panel and Figure 4E Si-NC MHY1485 panel, as well as a duplication between Figure 7B Sham and
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Heterogeneity of tethered agonist signaling in adhesion G protein-coupled receptors Cell Chem. Bio. (IF 8.6) Pub Date : 2024-04-11 Andrew N. Dates, Daniel T.D. Jones, Jeffrey S. Smith, Meredith A. Skiba, Maria F. Rich, Maggie M. Burruss, Andrew C. Kruse, Stephen C. Blacklow
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Hypothiocyanous acid reductase is critical for host colonization and infection by Streptococcus pneumoniae J. Biol. Chem. (IF 5.5) Pub Date : 2024-04-09 Heather L. Shearer, Michael J. Currie, Hannah N. Agnew, Claudia Trappetti, Frederick Stull, Paul E. Pace, James C. Paton, Renwick C.J. Dobson, Nina Dickerhof
The major human pathogen encounters the immune-derived oxidant hypothiocyanous acid (HOSCN) at sites of colonization and infection. We recently identified the pneumococcal hypothiocyanous acid reductase (Har), a member of the flavoprotein disulfide reductase enzyme family, and showed that it contributes to the HOSCN tolerance of . Here, we demonstrate in mouse models of pneumococcal infection that
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Genome engineering with Cas9 and AAV repair templates generates frequent concatemeric insertions of viral vectors Nat. Biotechnol. (IF 46.9) Pub Date : 2024-04-08 Fabian P. Suchy, Daiki Karigane, Yusuke Nakauchi, Maimi Higuchi, Jinyu Zhang, Katja Pekrun, Ian Hsu, Amy C. Fan, Toshinobu Nishimura, Carsten T. Charlesworth, Joydeep Bhadury, Toshiya Nishimura, Adam C. Wilkinson, Mark A. Kay, Ravindra Majeti, Hiromitsu Nakauchi
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Identification of an inhibitory pocket in falcilysin provides a new avenue for malaria drug development Cell Chem. Bio. (IF 8.6) Pub Date : 2024-04-08 Grennady Wirjanata, Jianqing Lin, Jerzy Michal Dziekan, Abbas El Sahili, Zara Chung, Seth Tjia, Nur Elyza Binte Zulkifli, Josephine Boentoro, Roy Tham, Lai Si Jia, Ka Diam Go, Han Yu, Anthony Partridge, David Olsen, Nayana Prabhu, Radoslaw M. Sobota, Pär Nordlund, Julien Lescar, Zbynek Bozdech
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A novel system to determine activity of individual uridine 5′-diphospho-glucuronosyltransferase (UGT) isoforms: Recombinant UGT-beads J. Biol. Chem. (IF 5.5) Pub Date : 2024-04-08 Ting Wang, Mitchell E. Taub, Tom S. Chan
Previous work demonstrated that human liver microsomes (HLMs) can spontaneously bind to silica-coated magnetizable beads (HLM-beads) and that these HLM-beads retain uridine 5′-diphospho-glucuronosyltransferase (UGT) activity. However, the contributions of individual UGT isoforms are not directly assessable in this system except through use of model inhibitors. Thus, a preparation wherein recombinant