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Correction to: SIRT7 antagonizes human stem cell aging as a heterochromatin stabilizer. Protein Cell (IF 21.1) Pub Date : 2023-07-15
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SARS-CoV-2 ORF8 does not function in the nucleus as a histone mimic. Protein Cell (IF 21.1) Pub Date : 2023-07-14 Ping Liu,Junjie Hu,Lei Wang
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Aging-induced YTHDF aggregates impair mitochondrial function by trapping mitochondrial RNAs and suppressing their expression in the brain. Protein Cell (IF 21.1) Pub Date : 2023-07-04 Juan Zhang,Dingfeng Li,Keqiang He,Qiang Liu,Zhongwen Xie
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A single-nucleus transcriptomic atlas of primate liver aging uncovers the pro-senescence role of SREBP2 in hepatocytes. Protein Cell (IF 21.1) Pub Date : 2023-06-28 Shanshan Yang,Chengyu Liu,Mengmeng Jiang,Xiaoqian Liu,Lingling Geng,Yiyuan Zhang,Shuhui Sun,Kang Wang,Jian Yin,Shuai Ma,Si Wang,Juan Carlos Izpisua Belmonte,Weiqi Zhang,Jing Qu,Guang-Hui Liu
Aging increases the risk of liver diseases and systemic susceptibility to aging-related diseases. However, cell-type-specific changes and the underlying mechanism of liver aging in higher vertebrates remain incompletely characterized. Here, we constructed the first single-nucleus transcriptomic landscape of primate liver aging, in which we resolved cell-type-specific gene expression fluctuation in
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DCK confers sensitivity of DCTD-positive cancer cells to oxidized methylcytidines. Protein Cell (IF 21.1) Pub Date : 2023-06-28 Ya-Hui Zhao,Wei Jiang,Hai Gao,Guo-Zheng Pang,Yu-Shuang Wu,Yuan-Xian Wang,Meng-Yao Sheng,Jia-Ying Xie,Wan-Ling Wu,Zhi-Jian Ji,Ya-Rui Du,Lei Zhang,Xiao-Qin Wang,Colum P Walsh,Hai Jiang,Guo-Liang Xu,Dan Zhou
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Drug repurposing screening and mechanism analysis based on human colorectal cancer organoids. Protein Cell (IF 21.1) Pub Date : 2023-06-22 Yunuo Mao,Wei Wang,Jingwei Yang,Xin Zhou,Yongqu Lu,Junpeng Gao,Xiao Wang,Lu Wen,Wei Fu,Fuchou Tang
Colorectal cancer (CRC) is a highly heterogeneous cancer and exploring novel therapeutic options is a pressing issue that needs to be addressed. Here, we established human CRC tumor-derived organoids that well represent both morphological and molecular heterogeneities of original tumors. To efficiently identify repurposed drugs for CRC, we developed a robust organoid-based drug screening system. By
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Single transmembrane GPCR modulating proteins: neither single nor simple. Protein Cell (IF 21.1) Pub Date : 2023-06-14 Meng Wang,Jianjun Lyu,Chao Zhang
The discovery of G-protein coupled receptor (GPCR) accessory proteins has fundamentally redefined the pharmacological concept of GPCR signaling, demonstrating a more complex molecular basis for receptor specificity on the plasma membrane and impressionable downstream intracellular cascades. GPCR accessory proteins not only contribute to the proper folding and trafficking of receptors, but also exhibit
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BMP7 expression in mammalian cortical radial glial cells increases the length of the neurogenic period. Protein Cell (IF 21.1) Pub Date : 2023-06-10 Zhenmeiyu Li,Guoping Liu,Lin Yang,Mengge Sun,Zhuangzhi Zhang,Zhejun Xu,Yanjing Gao,Xin Jiang,Zihao Su,Xiaosu Li,Zhengang Yang
The seat of human intelligence is the human cerebral cortex, which is responsible for our exceptional cognitive abilities. Identifying principles that lead to the development of the large-sized human cerebral cortex will shed light on what makes the human brain and species so special. The remarkable increase in the number of human cortical pyramidal neurons and the size of the human cerebral cortex
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An orally available monovalent SMAC mimetic compound as a broad-spectrum antiviral. Protein Cell (IF 21.1) Pub Date : 2023-06-09 Miao Mei,Maria Antonietta Impagnatiello,Jun Jiao,Ulrich Reiser,Ulrike Tontsch-Grunt,Ju Zhang,Paul Nicklin,Bingke Yu,Yu Wang,Yuan He,Xu Tan
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Biallelic variants in RBM42 cause a multisystem disorder with neurological, facial, cardiac, and musculoskeletal involvement. Protein Cell (IF 21.1) Pub Date : 2023-06-09 Yiyao Chen,Bingxin Yang,Xiaoyu Merlin Zhang,Songchang Chen,Minhui Wang,Liya Hu,Nina Pan,Shuyuan Li,Weihui Shi,Zhenhua Yang,Li Wang,Yajing Tan,Jian Wang,Yanlin Wang,Qinghe Xing,Zhonghua Ma,Jinsong Li,He-Feng Huang,Jinglan Zhang,Chenming Xu
Here, we report a previously unrecognized syndromic neurodevelopmental disorder associated with bi-allelic loss-of-function variants in the RBM42 gene. The patient is a two-year-old female with severe central nervous system abnormalities, hypotonia, hearing loss, congenital heart defects, and dysmorphic facial features. Familial whole-exome sequencing reveals that the patient has two compound heterozygous
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Spatially resolved expression landscape and gene-regulatory network of human gastric corpus epithelium. Protein Cell (IF 21.1) Pub Date : 2023-06-07 Ji Dong,Xinglong Wu,Xin Zhou,Yuan Gao,Changliang Wang,Wendong Wang,Weiya He,Jingyun Li,Wenjun Deng,Jiayu Liao,Xiaotian Wu,Yongqu Lu,Antony K Chen,Lu Wen,Wei Fu,Fuchou Tang
Molecular knowledge of human gastric corpus epithelium remains incomplete. Here, by integrated analyses using single-cell RNA sequencing (scRNA-seq), spatial transcriptomics, and single-cell assay for transposase accessible chromatin sequencing (scATAC-seq) techniques, we uncovered the spatially resolved expression landscape and gene-regulatory network of human gastric corpus epithelium. Specifically
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Human 8-cell embryos enable efficient induction of disease-preventive mutations without off-target effect by cytosine base editor. Protein Cell (IF 21.1) Pub Date : 2023-06-07 Yinghui Wei,Meiling Zhang,Jing Hu,Yingsi Zhou,Mingxing Xue,Jianhang Yin,Yuanhua Liu,Hu Feng,Ling Zhou,Zhifang Li,Dongshuang Wang,Zhiguo Zhang,Yin Zhou,Hongbin Liu,Ning Yao,Erwei Zuo,Jiazhi Hu,Yanzhi Du,Wen Li,Chunlong Xu,Hui Yang
Approximately 140 million people worldwide are homozygous carriers of APOE4 (ε4), a strong genetic risk factor for late onset familial and sporadic Alzheimer's disease (AD), 91% of whom will develop AD at earlier age than heterozygous carriers and noncarriers. Susceptibility to AD could be reduced by targeted editing of APOE4, but a technical basis for controlling the off-target effects of base editors
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Single-cell profiling reveals a potent role of quercetin in promoting hair regeneration. Protein Cell (IF 21.1) Pub Date : 2023-06-07 Qian Zhao,Yandong Zheng,Dongxin Zhao,Liyun Zhao,Lingling Geng,Shuai Ma,Yusheng Cai,Chengyu Liu,Yupeng Yan,Juan Carlos Izpisua Belmonte,Si Wang,Weiqi Zhang,Guang-Hui Liu,Jing Qu
Hair loss affects millions of people at some time in their life, and safe and efficient treatments for hair loss are a significant unmet medical need. We report that topical delivery of quercetin (Que) stimulates resting hair follicles to grow with rapid follicular keratinocyte proliferation and replenishes perifollicular microvasculature in mice. We construct dynamic single-cell transcriptome landscape
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Cryo-EM structure of human κ-opioid receptor-Gi complex bound to an endogenous agonist dynorphin A. Protein Cell (IF 21.1) Pub Date : 2023-06-07 Yuxiang Chen,Bo Chen,Tingting Wu,Fangfang Zhou,Fei Xu
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Innovative insights into extrachromosomal circular DNAs in gynecologic tumors and reproduction. Protein Cell (IF 21.1) Pub Date : 2023-05-26 Ning Wu,Ling Wei,Zhipeng Zhu,Qiang Liu,Kailong Li,Fengbiao Mao,Jie Qiao,Xiaolu Zhao
Originating but free from chromosomal DNA, extrachromosomal circular DNAs (eccDNAs) are organized in circular form and have long been found in unicellular and multicellular eukaryotes. Their biogenesis and function are poorly understood as they are characterized by sequence homology with linear DNA, for which few detection methods are available. Recent advances in high-throughput sequencing technologies
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Correction to: Enhancing prime editing efficiency and flexibility with tethered and split pegRNAs. Protein Cell (IF 21.1) Pub Date : 2023-05-25
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Microbiome research outlook: past, present, and future. Protein Cell (IF 21.1) Pub Date : 2023-05-23 Yunyun Gao,Danyi Li,Yong-Xin Liu
With its critical role in human health and disease, the microbiome has transformed modern biology. Over the past few years, microbiome research has evolved rapidly, with microbiologists gradually shifting their focus from cataloging microorganisms in the human microbiome to understanding their functional roles and how they interact with the host. Here, we present the global trends in microbiome research
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Deciphering the placental abnormalities associated with somatic cell nuclear transfer at single-nucleus resolution. Protein Cell (IF 21.1) Pub Date : 2023-05-19 Liyuan Jiang,Xin Wang,Leyun Wang,Sinan Ma,Yali Ding,Chao Liu,Siqi Wang,Xuan Shao,Ying Zhang,Zhikun Li,Wei Li,Guihai Feng,Qi Zhou
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Maternal and infant microbiome: next-generation indicators and targets for inter-generational health and nutrition care. Protein Cell (IF 21.1) Pub Date : 2023-05-15 Shengtao Gao,Jinfeng Wang
Microbes are commonly sensitive to shifts in the physiological and pathological state of their hosts, including mothers and babies. From this perspective, the microbiome may be a good indicator for diseases during pregnancy and has the potential to be used for perinatal health monitoring. This is embodied in the application of microbiome from multi body sites for auxiliary diagnosis, early prediction
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Human ESC-derived vascular cells promote vascular regeneration in a HIF-1α dependent manner. Protein Cell (IF 21.1) Pub Date : 2023-05-09 Jinghui Lei,Xiaoyu Jiang,Daoyuan Huang,Ying Jing,Shanshan Yang,Lingling Geng,Yupeng Yan,Fangshuo Zheng,Fang Cheng,Weiqi Zhang,Juan Carlos Izpisua Belmonte,Guang-Hui Liu,Si Wang,Jing Qu
Hypoxia-inducible factor (HIF-1α), a core transcription factor responding to changes in cellular oxygen levels, is closely associated with a wide range of physiological and pathological conditions. However, its differential impacts on vascular cells types and molecular programs modulating human vascular homeostasis and regeneration remain largely elusive. Here, we applied CRISPR/Cas9-mediated gene
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IDDoR: a novel reporter mouse system for simultaneous and quantitative in vivo analysis of both DNA double-strand break repair pathways. Protein Cell (IF 21.1) Pub Date : 2023-05-08 Yu Chen,Zhen Cui,Zhixi Chen,Ying Jiang,Zhiyong Mao
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Modeling human pregastrulation development by 3D culture of blastoids generated from primed-to-naïve transitioning intermediates. Protein Cell (IF 21.1) Pub Date : 2023-05-08 Zhifen Tu,Yan Bi,Xuehao Zhu,Wenqiang Liu,Jindian Hu,Li Wu,Tengyan Mao,Jianfeng Zhou,Hanwei Wang,Hong Wang,Shaorong Gao,Yixuan Wang
Human pluripotent stem cells provide an inexhaustible model to study human embryogenesis in vitro. Recent studies have provided diverse models to generate human blastoids by self-organization of different pluripotent stem cells or somatic reprogramming intermediates. However, whether blastoids can be generated from other cell types or whether they can recapitulate postimplantation development in vitro
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Single-cell analysis reveals an Angpt4-initiated EPDC-EC-CM cellular coordination cascade during heart regeneration. Protein Cell (IF 21.1) Pub Date : 2023-05-08 Zekai Wu,Yuan Shi,Yueli Cui,Xin Xing,Liya Zhang,Da Liu,Yutian Zhang,Ji Dong,Li Jin,Meijun Pang,Rui-Ping Xiao,Zuoyan Zhu,Jing-Wei Xiong,Xiangjun Tong,Yan Zhang,Shiqiang Wang,Fuchou Tang,Bo Zhang
Mammals exhibit limited heart regeneration ability, which can lead to heart failure after myocardial infarction. In contrast, zebrafish exhibit remarkable cardiac regeneration capacity. Several cell types and signaling pathways have been reported to participate in this process. However, a comprehensive analysis of how different cells and signals interact and coordinate to regulate cardiac regeneration
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Mechanism and design of allosteric activators of SIRT1. Protein Cell (IF 21.1) Pub Date : 2023-05-08 Fei Liu,Ningning Pang,Rui-Ming Xu,Na Yang
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Structural insights into signal transduction of the purinergic receptors P2Y1R and P2Y12R. Protein Cell (IF 21.1) Pub Date : 2023-05-08 Beibei Li,Shuo Han,Mu Wang,Yu Yu,Limin Ma,Xiaojing Chu,Qiuxiang Tan,Qiang Zhao,Beili Wu
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Cryo-EM structures of a prokaryotic heme transporter CydDC. Protein Cell (IF 21.1) Pub Date : 2023-05-05 Chen Zhu,Yanfeng Shi,Jing Yu,Wenhao Zhao,Lingqiao Li,Liang Jingxi,Xiaolin Yang,Bing Zhang,Yao Zhao,Yan Gao,Xiaobo Chen,Xiuna Yang,Lu Zhang,Luke W Guddat,Lei Liu,Haitao Yang,Zihe Rao,Jun Li
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The best practice for microbiome analysis using R. Protein Cell (IF 21.1) Pub Date : 2023-05-02 Tao Wen,Guoqing Niu,Tong Chen,Qirong Shen,Jun Yuan,Yong-Xin Liu
With the gradual maturity of sequencing technology, many microbiome studies have published, driving the emergence and advance of related analysis tools. R language is the widely used platform for microbiome data analysis for powerful functions. However, tens of thousands of R packages and numerous similar analysis tools have brought major challenges for many researchers to explore microbiome data.
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Nutri-microbiome epidemiology, an emerging field to disentangle the interplay between nutrition and microbiome for human health. Protein Cell (IF 21.1) Pub Date : 2023-04-26 Wanglong Gou,Zelei Miao,Kui Deng,Ju-Sheng Zheng
Diet and nutrition have a substantial impact on the human microbiome, and interact with the microbiome, especially gut microbiome, to modulate various diseases and health status. Microbiome research has also guided the nutrition field to a more integrative direction, becoming an essential component of the rising area of precision nutrition. In this review, we provide a broad insight into the interplay
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Impact of the microbiome on mosquito-borne diseases. Protein Cell (IF 21.1) Pub Date : 2023-04-25 Huicheng Shi,Xi Yu,Gong Cheng
Mosquito-borne diseases present a significant threat to human health, with the possibility of outbreaks of new mosquito-borne diseases always looming. Unfortunately, current measures to combat these diseases such as vaccines and drugs are often either unavailable or ineffective. However, recent studies on microbiomes may reveal promising strategies to fight these diseases. In this review, we examine
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Gut microbial methionine impacts circadian clock gene expression and reactive oxygen species level in host gastrointestinal tract. Protein Cell (IF 21.1) Pub Date : 2023-04-21 Xiaolin Liu,Yue Ma,Ying Yu,Wenhui Zhang,Jingjing Shi,Xuan Zhang,Min Dai,Yuhan Wang,Hao Zhang,Jiahe Zhang,Jianghua Shen,Faming Zhang,Moshi Song,Jun Wang
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Single-nucleus transcriptomics reveals a gatekeeper role for FOXP1 in primate cardiac aging. Protein Cell (IF 21.1) Pub Date : 2023-04-21 Yiyuan Zhang,Yandong Zheng,Si Wang,Yanling Fan,Yanxia Ye,Yaobin Jing,Zunpeng Liu,Shanshan Yang,Muzhao Xiong,Kuan Yang,Jinghao Hu,Shanshan Che,Qun Chu,Moshi Song,Guang-Hui Liu,Weiqi Zhang,Shuai Ma,Jing Qu
Aging poses a major risk factor for cardiovascular diseases, the leading cause of death in the aged population. However, the cell type-specific changes underlying cardiac aging are far from being clear. Here, we performed single-nucleus RNA-sequencing analysis of left ventricles from young and aged cynomolgus monkeys to define cell composition changes and transcriptomic alterations across different
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Bilineage embryo-like structure from EPS cells can produce live mice with tetraploid trophectoderm. Protein Cell (IF 21.1) Pub Date : 2023-04-21 Kuisheng Liu,Xiaocui Xu,Dandan Bai,Yanhe Li,Yalin Zhang,Yanping Jia,Mingyue Guo,Xiaoxiao Han,Yingdong Liu,Yifan Sheng,Xiaochen Kou,Yanhong Zhao,Jiqing Yin,Sheng Liu,Jiayu Chen,Hong Wang,Yixuan Wang,Wenqiang Liu,Shaorong Gao
Self-organized blastoids from extended pluripotent stem (EPS) cells possess enormous potential for investigating postimplantation embryo development and related diseases. However, the limited ability of postimplantation development of EPS-blastoids hinders its further application. In this study, single-cell transcriptomic analysis indicated that the "trophectoderm (TE)-like structure" of EPS-blastoids
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Systematic identification of CRISPR off-target effects by CROss-seq. Protein Cell (IF 21.1) Pub Date : 2023-04-21 Yan Li,Shengyao Zhi,Tong Wu,Hong-Xuan Chen,Rui Kang,Dong-Zhao Ma,Zhou Songyang,Chuan He,Puping Liang,Guan-Zheng Luo
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"Sentinel or accomplice" - gut microbiota and microglia cross-talk in disorders of gut-brain interaction. Protein Cell (IF 21.1) Pub Date : 2023-04-19 Haonan Zheng,Cunzheng Zhang,Jindong Zhang,Liping Duan
Abnormal brain-gut interaction is considered the core pathological mechanism behind the disorders of gut-brain interaction, in which the intestinal microbiota plays an important role. Microglia are the "sentinels" of the central nervous system, which participate in tissue damage caused by traumatic brain injury, resist central infection and participate in neurogenesis, and are involved in the occurrence
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The engagement of histone lysine methyltransferases with nucleosomes: structural basis, regulatory mechanisms, and therapeutic implications. Protein Cell (IF 21.1) Pub Date : 2023-04-13 Yanjing Li,Kexue Ge,Tingting Li,Run Cai,Yong Chen
Histone lysine methyltransferases (HKMTs) deposit methyl groups onto lysine residues on histones and play important roles in regulating chromatin structure and gene expression. The structures and functions of HKMTs have been extensively investigated in recent decades, significantly advancing our understanding of the dynamic regulation of histone methylation. Here, we review the recent progress in structural
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Suppression of USP8 sensitizes cells to ferroptosis via SQSTM1/p62-mediated ferritinophagy. Protein Cell (IF 21.1) Pub Date : 2023-04-13 Lihong Liu,Birong Zheng,Manhui Luo,Jie Du,Fang Yang,Chuanxin Huang,Zengyi Ma,Chunmei Li,Deyin Guo,Hong Peng
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Gαi1/3 mediation of Akt-mTOR activation is important for RSPO3-induced angiogenesis. Protein Cell (IF 21.1) Pub Date : 2023-04-13 Gang Xu,Li-Na Qi,Mei-Qing Zhang,Xue-Yun Li,Jin-Long Chai,Zhi-Qing Zhang,Xia Chen,Qian Wang,Ke-Ran Li,Cong Cao
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Determiners of cell fates: the tricarboxylic acid cycle versus the citrate-malate shuttle. Protein Cell (IF 21.1) Pub Date : 2023-04-13 Dong Guo,Haiyan He,Ying Meng,Shudi Luo,Zhimin Lu
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YME1L overexpression exerts pro-tumorigenic activity in glioma by promoting Gαi1 expression and Akt activation. Protein Cell (IF 21.1) Pub Date : 2023-04-13 Fang Liu,Gang Chen,Li-Na Zhou,Yin Wang,Zhi-Qing Zhang,Xihu Qin,Cong Cao
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Architecture of severe fever with thrombocytopenia syndrome virus. Protein Cell (IF 21.1) Pub Date : 2023-04-11 Zixian Sun,Jing Cheng,Yuan Bai,Lin Cao,Daoxin Xie,Fei Deng,Xinzheng Zhang,Zihe Rao,Zhiyong Lou
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Structure basis for allosteric regulation of lymphocytic choriomeningitis virus polymerase function by Z matrix protein. Protein Cell (IF 21.1) Pub Date : 2023-04-11 Lu Liu,Panpan Wang,Aijun Liu,Leike Zhang,Liming Yan,Yu Guo,Gengfu Xiao,Zihe Rao,Zhiyong Lou
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Immunogenic molecules associated with gut bacterial cell walls: chemical structures, immune-modulating functions, and mechanisms. Protein Cell (IF 21.1) Pub Date : 2023-04-04 Ruopeng Yin,Tao Wang,Huanqin Dai,Junjie Han,Jingzu Sun,Ningning Liu,Wang Dong,Jin Zhong,Hongwei Liu
Interactions between gut microbiome and host immune system are fundamental to maintaining the intestinal mucosal barrier and homeostasis. At the host-gut microbiome interface, cell wall-derived molecules from gut commensal bacteria have been reported to play a pivotal role in training and remodeling host immune responses. In this article, we review gut bacterial cell wall-derived molecules with characterized
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APOE-mediated suppression of the lncRNA MEG3 protects human cardiovascular cells from chronic inflammation. Protein Cell (IF 21.1) Pub Date : 2023-04-03 Hongkai Zhao,Kuan Yang,Yiyuan Zhang,Hongyu Li,Qianzhao Ji,Zeming Wu,Shuai Ma,Si Wang,Moshi Song,Guang-Hui Liu,Qiang Liu,Weiqi Zhang,Jing Qu
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Emerging applications of tumour-educated platelets (TEPs) in the detection and prognostication of ovarian cancer. Protein Cell (IF 21.1) Pub Date : 2023-03-27 Jie Wei Zhu,Vasily Giannakeas,Steven A Narod,Mohammad R Akbari
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NDFIP1 limits cellular TAZ accumulation via exosomal sorting to inhibit NSCLC proliferation. Protein Cell (IF 21.1) Pub Date : 2023-03-16 Yirui Cheng,Xin Lu,Fan Li,Zhuo Chen,Yanshuang Zhang,Qing Han,Qingyu Zeng,Tingyu Wu,Ziming Li,Shun Lu,Cecilia Williams,Weiliang Xia
NDFIP1 has been previously reported as a tumor suppressor in multiple solid tumors, but the function of NDFIP1 in NSCLC and the underlying mechanism are still unknown. Besides, the WW domain containing proteins can be recognized by NDFIP1, resulted in the loading of the target proteins into exosomes. However, whether WW domain-containing transcription regulator 1 (WWTR1, also known as TAZ) can be packaged
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Phosphorylation of NF2 at Serine-13 by MAP4K family kinases mediates pathological angiogenesis. Protein Cell (IF 21.1) Pub Date : 2023-03-16 Mingyue Ma,Zhenxing Zhong,Yuwen Zhu,Yuan Gu,Ruxin Jin,Zhipeng Meng,Yu Wang,Fa-Xing Yu
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Resolving the lineage relationship between malignant cells and vascular cells in glioblastomas. Protein Cell (IF 21.1) Pub Date : 2023-03-16 Fangyu Wang,Xuan Liu,Shaowen Li,Chen Zhao,Yumei Sun,Kuan Tian,Junbao Wang,Wei Li,Lichao Xu,Jing Jing,Juan Wang,Sylvia M Evans,Zhiqiang Li,Ying Liu,Yan Zhou
Glioblastoma multiforme (GBM), a highly malignant and heterogeneous brain tumor, contains various types of tumor and non-tumor cells. Whether GBM cells can trans-differentiate into non-neural cell types, including mural cells or endothelial cells (ECs), to support tumor growth and invasion remains controversial. Here we generated two genetic GBM models de novo in immunocompetent mouse brains, mimicking
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How erastin assassinates cells by ferroptosis revealed. Protein Cell (IF 21.1) Pub Date : 2023-03-16 Boyi Gan
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RNA helicase MTR4 drives tumorigenesis of nasopharyngeal carcinoma by regulating the expression of key cell cycle genes. Protein Cell (IF 21.1) Pub Date : 2023-03-16 Lili Yu,Lei Jiang,Meng Wu,Wenlong Dou,Kaiyuan Ji,Jianlong Zhou,Jinchul Kim,Yang Xu
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Identification of lipid droplets in gut bacteria. Protein Cell (IF 21.1) Pub Date : 2023-03-16 Kai Zhang,Chang Zhou,Zemin Li,Xuehan Li,Ziyun Zhou,Linjia Cheng,Ahmed Hammad Mirza,Yumeng Shi,Bingbing Chen,Mengwei Zhang,Liujuan Cui,Congyan Zhang,Taotao Wei,Xuelin Zhang,Shuyan Zhang,Pingsheng Liu
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A long non-coding RNA LncSync regulates mouse cardiomyocyte homeostasis and cardiac hypertrophy through coordination of miRNA actions. Protein Cell (IF 21.1) Pub Date : 2023-03-16 Rujin Huang,Jinyang Liu,Xi Chen,Ying Zhi,Shuangyuan Ding,Jia Ming,Yulin Li,Yangming Wang,Jie Na
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Immunology of a unique biological structure: the Echinococcus laminated layer. Protein Cell (IF 21.1) Pub Date : 2023-03-16 Álvaro Díaz,Anabella A Barrios,Leticia Grezzi,Camila Mouhape,Stephen J Jenkins,Judith E Allen,Cecilia Casaravilla
The larval stages of the cestode parasites belonging to the genus Echinococcus grow within internal organs of humans and a range of animal species. The resulting diseases, collectively termed echinococcoses, include major neglected tropical diseases of humans and livestock. Echinococcus larvae are outwardly protected by the laminated layer (LL), an acellular structure that is unique to this genus.
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Strategies for generating mouse model resources of human disease. Protein Cell (IF 21.1) Pub Date : 2023-03-14 Jirong Pan,Ling Zhang,Zhibing Huang,Dalu Zhao,He Li,Yanan Fu,Meng Wang,Borui Chen,Iraqi Fuad A,Morahan Grant,Chuan Qin
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CRISPR-assisted transcription activation by phase separation proteins. Protein Cell (IF 21.1) Pub Date : 2023-03-11 Jiaqi Liu,Yuxi Chen,Baoting Nong,Xiao Luo,Kaixin Cui,Wenqiong Tan,Yue Yang,Wenbin Ma,Puping Liang,Zhou Songyang
The clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 system has been widely used for genome engineering and transcriptional regulation in many different organisms. Current CRISPR-activation (CRISPRa) platforms often require multiple components because of inefficient transcriptional activation. Here, we fused different phase-separation proteins to dCas9-VPR (dCas9-VP64-P65-RTA)
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CRISPR-based screening identifies XPO7 as a positive regulator of senescence. Protein Cell (IF 21.1) Pub Date : 2023-03-10 Lan-Zhu Li,Kuan Yang,Yaobin Jing,Yanling Fan,Xiaoyu Jiang,Si Wang,Guang-Hui Liu,Jing Qu,Shuai Ma,Weiqi Zhang
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METTL14 is a chromatin regulator independent of its RNA N6-methyladenosine methyltransferase activity. Protein Cell (IF 21.1) Pub Date : 2023-02-23 Xiaoyang Dou,Lulu Huang,Yu Xiao,Chang Liu,Yini Li,Xinning Zhang,Lishan Yu,Ran Zhao,Lei Yang,Chuan Chen,Xianbin Yu,Boyang Gao,Meijie Qi,Yawei Gao,Bin Shen,Shuying Sun,Chuan He,Jun Liu
METTL3 and METTL14 are two components that form the core heterodimer of the main RNA m6A methyltransferase complex (MTC) that installs m6A. Surprisingly, depletion of METTL3 or METTL14 displayed distinct effects on stemness maintenance of mouse embryonic stem cell (mESC). While comparable global hypo-methylation in RNA m6A was observed in Mettl3 or Mettl14 knockout mESCs, respectively. Mettl14 knockout
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Single-cell profiling reveals Müller glia coordinate retinal intercellular communication during light/dark adaptation via thyroid hormone signaling. Protein Cell (IF 21.1) Pub Date : 2023-02-21 Min Wei,Yanping Sun,Shouzhen Li,Yunuo Chen,Longfei Li,Minghao Fang,Ronghua Shi,Dali Tong,Jutao Chen,Yuqian Ma,Kun Qu,Mei Zhang,Tian Xue
Light adaptation enables the vertebrate visual system to operate over a wide range of ambient illumination. Regulation of phototransduction in photoreceptors is considered a major mechanism underlying light adaptation. However, various types of neurons and glial cells exist in the retina, and whether and how all retinal cells interact to adapt to light/dark conditions at the cellular and molecular
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Dual role of lipids for genome stability and pluripotency facilitates full potency of mouse embryonic stem cells. Protein Cell (IF 21.1) Pub Date : 2023-02-16 Liangwen Zhong,Miriam Gordillo,Xingyi Wang,Yiren Qin,Yuanyuan Huang,Alexey Soshnev,Ritu Kumar,Gouri Nanjangud,Daylon James,C David Allis,Todd Evans,Bryce Carey,Duancheng Wen
While Mek1/2 and Gsk3β inhibition ("2i") supports the maintenance of murine embryonic stem cells (ESCs) in a homogenous naïve state, prolonged culture in 2i results in aneuploidy and DNA hypomethylation that impairs developmental potential. Additionally, 2i fails to support derivation and culture of fully potent female ESCs. Here we find that mouse ESCs cultured in 2i/LIF supplemented with lipid-rich
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Face off: a metabolic enzyme becomes a protein phosphatase. Protein Cell (IF 21.1) Pub Date : 2023-06-28 Gaoxiang Zhao,Qian Lin,Zhaoyuan Meng,Xinlei Sheng,Leina Ma,Yingming Zhao
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Recent progress and challenges in the treatment of spinal cord injury. Protein Cell (IF 21.1) Pub Date : 2023-02-10 Ting Tian,Sensen Zhang,Maojun Yang
Spinal cord injury (SCI) disrupts the structural and functional connectivity between the higher center and the spinal cord, resulting in severe motor, sensory, and autonomic dysfunction with a variety of complications. The pathophysiology of SCI is complicated and multifaceted, and thus individual treatments acting on a specific aspect or process are inadequate to elicit neuronal regeneration and functional