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An efficient genetic transformation system mediated by Rhizobium rhizogenes in fruit trees based on the transgenic hairy root to shoot conversion Plant Biotech. J. (IF 13.8) Pub Date : 2024-03-16 Lin Liu, Jinghua Qu, Chunyan Wang, Miao Liu, Chunmeng Zhang, Xinyue Zhang, Cheng Guo, Changai Wu, Guodong Yang, Jinguang Huang, Kang Yan, Huairui Shu, Chengchao Zheng, Shizhong Zhang
SummaryGenetic transformation is a critical tool for gene editing and genetic improvement of plants. Although many model plants and crops can be genetically manipulated, genetic transformation systems for fruit trees are either lacking or perform poorly. We used Rhizobium rhizogenes to transfer the target gene into the hairy roots of Malus domestica and Actinidia chinensis. Transgenic roots were generated
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PagARGOS promotes low‐lignin wood formation in poplar Plant Biotech. J. (IF 13.8) Pub Date : 2024-03-16 Xiaomin Yao, Guifang Zhang, Geng Zhang, Qian Sun, Cuimei Liu, Jinfang Chu, Yanping Jing, Shihui Niu, Chunxiang Fu, Tedrick Thomas Salim Lew, Jinxing Lin, Xiaojuan Li
SummaryWood formation, which occurs mainly through secondary xylem development, is important not only for supplying raw material for the ‘ligno‐chemical’ industry but also for driving the storage of carbon. However, the complex mechanisms underlying the promotion of xylem formation remain to be elucidated. Here, we found that overexpression of Auxin‐Regulated Gene involved in Organ Size (ARGOS) in
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A Verticillium dahliae exoglucanase as potential HIGS target interacts with a cotton cysteine protease to confer resistance to cotton Verticillium wilt Plant Biotech. J. (IF 13.8) Pub Date : 2024-03-16 Xiaofeng Su, Qi Wang, Tao Zhang, Xiaoyang Ge, Wende Liu, Huiming Guo, Xingfen Wang, Zhengwen Sun, Zhiqiang Li, Hongmei Cheng
Verticillium wilt, caused by the soil-borne pathogenic fungus Verticillium dahliae (Vd), represents a devastating disease impacting cotton (Gossypium spp.). However, the limited efficacy of measures to control Verticillium wilt arises because Vd colonizes the host vascular system, as well as the inherent resilience of Vd resting structures (microsclerotia), to various environmental influences (Fradin
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Development of a transgenic rice line with strong and broad resistance against four devastating rice viruses through expressing a single hairpin RNA construct Plant Biotech. J. (IF 13.8) Pub Date : 2024-03-16 Chenyang Li, Jianxiang Wu, Shuai Fu, Yi Xu, Yaqin Wang, Xiuling Yang, Ying Lan, Feng Lin, Linlin Du, Tong Zhou, Xueping Zhou
Rice is a staple food crop feeding nearly half of the world's population. However, rice production is threatened by devastating viral diseases such as rice black-streaked dwarf virus (RBSDV), southern rice black-streaked dwarf virus (SRBSDV), rice stripe virus (RSV) and rice ragged stunt virus (RRSV) (Wang et al., 2022a). Traditional breeding for viral resistance is challenging due to the lack of natural
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Recessive resistance against beet chlorosis virus is conferred by the eukaryotic translation initiation factor (iso)4E in Beta vulgaris Plant Biotech. J. (IF 13.8) Pub Date : 2024-03-16 Lukas Rollwage, Hilde Van Houtte, Roxana Hossain, Niels Wynant, Glenda Willems, Mark Varrelmann
SummaryEukaryotic translation initiation factors (eIFs) are important for mRNA translation but also pivotal for plant‐virus interaction. Most of these plant‐virus interactions were found between plant eIFs and the viral protein genome‐linked (VPg) of potyviruses. In case of lost interaction due to mutation or deletion of eIFs, the viral translation and subsequent replication within its host is negatively
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PTI‐ETI synergistic signal mechanisms in plant immunity Plant Biotech. J. (IF 13.8) Pub Date : 2024-03-12 Xiao‐Qian Yu, Hao‐Qiang Niu, Chao Liu, Hou‐Ling Wang, Weilun Yin, Xinli Xia
SummaryPlants face a relentless onslaught from a diverse array of pathogens in their natural environment, to which they have evolved a myriad of strategies that unfold across various temporal scales. Cell surface pattern recognition receptors (PRRs) detect conserved elicitors from pathogens or endogenous molecules released during pathogen invasion, initiating the first line of defence in plants, known
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Knockout of GRAIN WIDTH2 has a dual effect on enhancing leaf rust resistance and increasing grain weight in wheat Plant Biotech. J. (IF 13.8) Pub Date : 2024-03-09 Shujuan Liu, Hong Liu, Mengjiao Guo, Yuxue Pan, Chenyang Hao, Jian Hou, Liuling Yan, Xueyong Zhang, Xinhong Chen, Tian Li
Wheat leaf rust, caused by the fungal pathogen Puccinia triticina Eriksson (Pt), poses a major threat to global wheat production. The widespread presence and rapid evolution of Pt races lead to frequent epidemics, particularly in favourable climates, causing yield losses of 30%–50% (Prasad et al., 2020). Employing resistant genes and cultivars remains the most effective strategy against leaf rust.
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miR396b/GRF6 module contributes to salt tolerance in rice Plant Biotech. J. (IF 13.8) Pub Date : 2024-03-08 Huanran Yuan, Mingxing Cheng, Ruihua Wang, Zhikai Wang, Fengfeng Fan, Wei Wang, Fengfeng Si, Feng Gao, Shaoqing Li
SummarySalinity, as one of the most challenging environmental factors restraining crop growth and yield, poses a severe threat to global food security. To address the rising food demand, it is urgent to develop crop varieties with enhanced yield and greater salt tolerance by delving into genes associated with salt tolerance and high‐yield traits. MiR396b/GRF6 module has previously been demonstrated
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High‐throughput selective sweep SNP‐guided cloning of cold‐tolerance genes in rice Plant Biotech. J. (IF 13.8) Pub Date : 2024-03-07 Xiaoxia Li, Hongmei Wang, Chujian Xiao, Juan Huang, Yuting Long, Chuxin Lin, Yue Zhu, Man Wang, Yao‐Guang Liu, Qunyu Zhang
The genetic dissection and cloning of quantitative trait loci (QTLs) are prerequisites for implementing genomics-based applications in breeding programs. However, traditional positional cloning for QTLs is difficult, laborious and time-consuming. For example, more than 250 and 600 QTLs for cold tolerance have been detected in rice using genetic mapping and genome-wide association studies, respectively
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A higher‐yield hybrid rice is achieved by assimilating a dominant heterotic gene in inbred parental lines Plant Biotech. J. (IF 13.8) Pub Date : 2024-03-07 Changsheng Wang, Ziqun Wang, Yunxiao Cai, Zhou Zhu, Danheng Yu, Lei Hong, Yongchun Wang, Wei Lv, Qiang Zhao, Lizhen Si, Kun Liu, Bin Han
SummaryThe exploitation of heterosis to integrate parental advantages is one of the fastest and most efficient ways of rice breeding. The genomic architecture of heterosis suggests that the grain yield is strongly correlated with the accumulation of numerous rare superior alleles with positive dominance. However, the improvements in yield of hybrid rice have shown a slowdown or even plateaued due to
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CaIAA2‐CaARF9 module mediates the trade‐off between pepper growth and immunity Plant Biotech. J. (IF 13.8) Pub Date : 2024-03-07 Weiwei Cai, Yilin Tao, Xingge Cheng, Meiyun Wan, Jianghuang Gan, Sheng Yang, Thomas W. Okita, Shuilin He, Li Tian
SummaryTo challenge the invasion of various pathogens, plants re‐direct their resources from plant growth to an innate immune defence system. However, the underlying mechanism that coordinates the induction of the host immune response and the suppression of plant growth remains unclear. Here we demonstrate that an auxin response factor, CaARF9, has dual roles in enhancing the immune resistance to Ralstonia
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Comparisons of wild and cultivated American ginseng (Panax quinquefolius L.) genomes provide insights into changes in root growth and metabolism during domestication Plant Biotech. J. (IF 13.8) Pub Date : 2024-03-06 Zhengpeng Wang, Tengteng Wang, Jin Hu, Honghong Jiao, Yan Jin, Jiahui Sun, Tiegui Nan, Yuyang Zhao, Yunjun Liu, Luqi Huang, Yuan Yuan
American ginseng (Panax quinquefolius L.) originated in the forests of North America and was introduced to China over 40 years ago. Cultivated P. quinquefolius in China had fewer lateral roots and significantly decreased ginsenoside Rg1 content compared to wild American varieties, both of which determines quality and yield of P. quinquefolius (Figure S1). To explore genetic basis to alterations in
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A near complete genome of Arachis monticola, an allotetraploid wild peanut Plant Biotech. J. (IF 13.8) Pub Date : 2024-03-04 Hongzhang Xue, Kai Zhao, Kunkun Zhao, Suoyi Han, Annapurna Chitikineni, Lin Zhang, Ding Qiu, Rui Ren, Fangping Gong, Zhongfeng Li, Xingli Ma, Xingguo Zhang, Rajeev K. Varshney, Xinyou Zhang, Chaochun Wei, Dongmei Yin
Peanut (Arachis hypogaea L.) is the most economically important oilseed legume crops throughout the world. Most Arachis species are diploid, but two are heterotetraploid: one cultivated species (A. hypogaea) and one wild relative (A. monticola). A. monticola was an important intermediate between diploid wild peanut species and tetraploid cultivated peanut varieties (Zhuang et al., 2019). We released
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Control of two insect pests by expression of a mismatch corrected double‐stranded RNA in plants Plant Biotech. J. (IF 13.8) Pub Date : 2024-03-01 Yi Dong, Qi Zhang, Yarou Mao, Mengting Wu, Zican Wang, Ling Chang, Jiang Zhang
SummaryRNA interference (RNAi) has emerged as an efficient technology for pest control by silencing the essential genes of targeted insects. Owing to its nucleotide sequence‐guided working mechanism, RNAi has a high degree of species‐specificity without impacts on non‐target organisms. However, as plants are inevitably under threat by two or more insect pests in nature, the species‐specific mode of
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A method of genetic transformation and gene editing of succulents without tissue culture Plant Biotech. J. (IF 13.8) Pub Date : 2024-03-01 Jinghua Lu, Shanshan Li, Shuai Deng, Mugui Wang, Yinghuang Wu, Ming Li, Jinsong Dong, Suhui Lu, Chunli Su, Guofu Li, Zhaobo Lang, Jian‐Kang Zhu
SummarySucculents, valued for their drought tolerance and ornamental appeal, are important in the floriculture market. However, only a handful of succulent species can be genetically transformed, making it difficult to improve these plants through genetic engineering. In this study, we adapted the recently developed cut‐dip‐budding (CDB) gene delivery system to transform three previously recalcitrant
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Temperature and light reverse the fertility of rice P/TGMS line ostms19 via reactive oxygen species homeostasis Plant Biotech. J. (IF 13.8) Pub Date : 2024-02-29 Lei Zhou, Yi‐Chen Mao, Yan‐Ming Yang, Jun‐Jie Wang, Xiang Zhong, Yu Han, Yan‐Fei Zhang, Qiang‐Sheng Shi, Xue‐hui Huang, Blake C. Meyers, Jun Zhu, Zhong‐Nan Yang
SummaryP/TGMS (Photo/thermo‐sensitive genic male sterile) lines are crucial resources for two‐line hybrid rice breeding. Previous studies revealed that slow development is a general mechanism for sterility–fertility conversion of P/TGMS in Arabidopsis. However, the difference in P/TGMS genes between rice and Arabidopsis suggests the presence of a distinct P/TGMS mechanism in rice. In this study, we
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A 5.2‐kb insertion in the coding sequence of PavSCPL, a serine carboxypeptidase‐like enhances fruit firmness in Prunus avium Plant Biotech. J. (IF 13.8) Pub Date : 2024-02-28 Xiliang Qi, Yuanxin Dong, Congli Liu, Lulu Song, Lei Chen, Ming Li
SummaryFruit firmness is an important trait in sweet cherry breeding because it directly positively influences fruit transportability, storage and shelf life. However, the underlying genes responsible and the molecular mechanisms that control fruit firmness remain unknown. In this study, we identified a candidate gene, PavSCPL, encoding a serine carboxypeptidase‐like protein with natural allelic variation
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Genome editing of five starch synthesis genes produces highly resistant starch and dietary fibre in barley grains Plant Biotech. J. (IF 13.8) Pub Date : 2024-02-28 Qiang Yang, Jean‐Philippe Ral, Yuming Wei, Youliang Zheng, Zhongyi Li, Qiantao Jiang
Resistant starch (RS) refers to starch that is not digested in the stomach or small intestine, and provides health benefits by reducing glycaemic index and promoting gut health (Hazard et al., 2020). Barley (Hordeum vulgare L.) is the fourth most widely cultivated cereal worldwide, and there is growing interest in barley as a healthy food. The RS content of cereal grains is positively associated with
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Revisiting growth–defence trade‐offs and breeding strategies in crops Plant Biotech. J. (IF 13.8) Pub Date : 2024-02-27 Mingjun Gao, Zeyun Hao, Yuese Ning, Zuhua He
SummaryPlants have evolved a multi‐layered immune system to fight off pathogens. However, immune activation is costly and is often associated with growth and development penalty. In crops, yield is the main breeding target and is usually affected by high disease resistance. Therefore, proper balance between growth and defence is critical for achieving efficient crop improvement. This review highlights
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The TaSnRK1‐TabHLH489 module integrates brassinosteroid and sugar signalling to regulate the grain length in bread wheat Plant Biotech. J. (IF 13.8) Pub Date : 2024-02-27 Jinyang Lyu, Dongzhi Wang, Na Sun, Fan Yang, Xuepeng Li, Junyi Mu, Runxiang Zhou, Guolan Zheng, Xin Yang, Chenxuan Zhang, Chao Han, Guang‐Min Xia, Genying Li, Min Fan, Jun Xiao, Ming‐Yi Bai
SummaryRegulation of grain size is a crucial strategy for improving the crop yield and is also a fundamental aspect of developmental biology. However, the underlying molecular mechanisms governing grain development in wheat remain largely unknown. In this study, we identified a wheat atypical basic helix–loop–helix (bHLH) transcription factor, TabHLH489, which is tightly associated with grain length
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Mitochondrial genome editing of WA352 via mitoTALENs restore fertility in cytoplasmic male sterile rice Plant Biotech. J. (IF 13.8) Pub Date : 2024-02-27 Jiawei Zhou, Liyun Nie, Shuo Zhang, Hailiang Mao, Shin-ichi Arimura, Shuangxia Jin, Zhiqiang Wu
Cytoplasmic male sterility (CMS) is the inability of plants to produce functional pollen due to nucleo-cytoplasmic interaction (Wang et al., 2021). The CMS line is widely used in the three-line (CMS, maintainer and restorer) system to produce hybrid seeds with heterosis (Chen and Liu, 2014). Rice is one of the three major food crops in the world, and CMS-based hybrid rice technology has greatly improved
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Beyond pathways: Accelerated flavonoids candidate identification and novel exploration of enzymatic properties using combined mapping populations of wheat Plant Biotech. J. (IF 13.8) Pub Date : 2024-02-26 Jie Chen, Yueqi Zhang, Jiaqi Wei, Xin Hu, Huanran Yin, Wei Liu, Dongqin Li, Wenfei Tian, Yuanfeng Hao, Zhonghu He, Alisdair R. Fernie, Wei Chen
SummaryAlthough forward‐genetics‐metabolomics methods such as mGWAS and mQTL have proven effective in providing myriad loci affecting metabolite contents, they are somehow constrained by their respective constitutional flaws such as the hidden population structure for GWAS and insufficient recombinant rate for QTL. Here, the combination of mGWAS and mQTL was performed, conveying an improved statistical
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Optimizing ex vitro one‐step RUBY‐equipped hairy root transformation in drug‐ and hemp‐type Cannabis Plant Biotech. J. (IF 13.8) Pub Date : 2024-02-24 Ladan Ajdanian, Mohsen Niazian, Davoud Torkamaneh
Cannabis (Cannabis sativa L.), once concealed by the veil of prohibition, is now emerging as a versatile and promising plant species, riding the wave of recent legalization. This transformation has unlocked unprecedented opportunities for both medical research and industry growth, positioning cannabis on a trajectory to reach a projected market size of USD 444.34 Billion by 2030 (fortune business insights)
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ScRNA‐seq reveals dark‐ and light‐induced differentially expressed gene atlases of seedling leaves in Arachis hypogaea L. Plant Biotech. J. (IF 13.8) Pub Date : 2024-02-23 Quanqing Deng, Puxuan Du, Sunil S. Gangurde, Yanbin Hong, Yuan Xiao, Dongxiu Hu, Haifen Li, Qing Lu, Shaoxiong Li, Haiyan Liu, Runfeng Wang, Lu Huang, Wenyi Wang, Vanika Garg, Xuanqiang Liang, Rajeev K. Varshney, Xiaoping Chen, Hao Liu
SummaryAlthough the regulatory mechanisms of dark and light‐induced plant morphogenesis have been broadly investigated, the biological process in peanuts has not been systematically explored on single‐cell resolution. Herein, 10 cell clusters were characterized using scRNA‐seq‐identified marker genes, based on 13 409 and 11 296 single cells from 1‐week‐old peanut seedling leaves grown under dark and
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Exploring silique number in Brassica napus L.: Genetic and molecular advances for improving yield Plant Biotech. J. (IF 13.8) Pub Date : 2024-02-22 Hui Wang, Xiaodong Li, Boyu Meng, Yonghai Fan, Shahid Ullah Khan, Mingchao Qian, Minghao Zhang, Haikun Yang, Kun Lu
SummarySilique number is a crucial yield‐related trait for the genetic enhancement of rapeseed (Brassica napus L.). The intricate molecular process governing the regulation of silique number involves various factors. Despite advancements in understanding the mechanisms regulating silique number in Arabidopsis (Arabidopsis thaliana) and rice (Oryza sativa), the molecular processes involved in controlling
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A rapid and scalable approach to build synthetic repetitive hormone‐responsive promoters Plant Biotech. J. (IF 13.8) Pub Date : 2024-02-21 Josefina‐Patricia Fernandez‐Moreno, Anna E. Yaschenko, Matthew Neubauer, Alex J. Marchi, Chengsong Zhao, José T. Ascencio‐Ibanez, Jose M. Alonso, Anna N. Stepanova
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T‐circle vector strategy increases NHEJ‐mediated site‐specific integration in soybean Plant Biotech. J. (IF 13.8) Pub Date : 2024-02-19 Xudong Ye, John Bradley, Larry Gilbertson
During Agrobacterium-mediated transformation, linear single-stranded DNA (T-strand) carrying genes of interest (GOIs) from a right border (RB) to a left border (LB) in the transfer DNA (T-DNA) of a binary vector is transferred into plant cells and randomly integrated into the plant genome (Gelvin, 2021). Circular T-DNA molecules (T-circles) in plant cells have been reported (Bakkeren et al., 1989)
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High-quality chromosome-level genome assembly and multi-omics analysis of rosemary (Salvia rosmarinus) reveals new insights into the environmental and genome adaptation Plant Biotech. J. (IF 13.8) Pub Date : 2024-02-16 Yong Lai, Jinghua Ma, Xuebin Zhang, Xiaobo Xuan, Fengyun Zhu, Shen Ding, Fude Shang, Yuanyuan Chen, Bing Zhao, Chen Lan, Turgay Unver, George Huo, Ximei Li, Yihan Wang, Yufang Liu, Mengfei Lu, Xiaoping Pan, Deshuang Yang, Mingwan Li, Baohong Zhang, Dangquan Zhang
High-quality genome of rosemary (Salvia rosmarinus) represents a valuable resource and tool for understanding genome evolution and environmental adaptation as well as its genetic improvement. However, the existing rosemary genome did not provide insights into the relationship between antioxidant components and environmental adaptability. In this study, by employing Nanopore sequencing and Hi-C technologies
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CRISPR-targeted mutagenesis of mitogen-activated protein kinase phosphatase 1 improves both immunity and yield in wheat Plant Biotech. J. (IF 13.8) Pub Date : 2024-02-16 Saifei Liu, Fengfeng Zhang, Jiaxuan Su, Anfei Fang, Binnian Tian, Yang Yu, Chaowei Bi, Dongfang Ma, Shunyuan Xiao, Yuheng Yang
Plants have evolved a sophisticated immunity system for specific detection of pathogens and rapid induction of measured defences. Over- or constitutive activation of defences would negatively affect plant growth and development. Hence, the plant immune system is under tight positive and negative regulation. MAP kinase phosphatase1 (MKP1) has been identified as a negative regulator of plant immunity
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Lipid transfer protein StLTPa enhances potato disease resistance against different pathogens by binding and disturbing the integrity of pathogens plasma membrane Plant Biotech. J. (IF 13.8) Pub Date : 2024-02-16 Xiaokang Chen, Jiashu Feng, Zhenzhen Li, Hui Feng, Chunxu Song, Lin Cai, Matthieu H. A. J. Joosten, Yu Du
Potato is the third most important food crop worldwide. Potato production suffers from severe diseases caused by multiple detrimental plant pathogens, and broad-spectrum disease resistance genes are rarely identified in potato. Here we identified the potato non-specific lipid transfer protein StLTPa, which enhances species none-specific disease resistance against various pathogens, such as the oomycete
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Programmable RNA N6-methyladenosine editing with CRISPR/dCas13a in plants Plant Biotech. J. (IF 13.8) Pub Date : 2024-02-16 Chuanlin Shi, Wenli Zou, Xiangpei Liu, Hong Zhang, Xiaofang Li, Guiling Fu, Qili Fei, Qian Qian, Lianguang Shang
N6-methyladenonsine (m6A) is the most prevalent internal modification of messenger RNA (mRNA) and plays critical roles in mRNA processing and metabolism. However, perturbation of individual m6A modification to reveal its function and the phenotypic effects is still lacking in plants. Here, we describe the construction and characterization of programmable m6A editing tools by fusing the m6A writers
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GhRCD1 promotes cotton tolerance to cadmium by regulating the GhbHLH12–GhMYB44–GhHMA1 transcriptional cascade Plant Biotech. J. (IF 13.8) Pub Date : 2024-02-13 Xi Wei, Menghan Geng, Jiachen Yuan, Jingjing Zhan, Lisen Liu, Yanli Chen, Ye Wang, Wenqiang Qin, Hongying Duan, Hang Zhao, Fuguang Li, Xiaoyang Ge
Heavy metal pollution poses a significant risk to human health and wreaks havoc on agricultural productivity. Phytoremediation, a plant-based, environmentally benign, and cost-effective method, is employed to remove heavy metals from contaminated soil, particularly in agricultural or heavy metal-sensitive lands. However, the phytoremediation capacity of various plant species and germplasm resources
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Fusarium graminearum rapid alkalinization factor peptide negatively regulates plant immunity and cell growth via the FERONIA receptor kinase Plant Biotech. J. (IF 13.8) Pub Date : 2024-02-12 Yujie Wang, Xin Liu, Bingqin Yuan, Xue Chen, Hanxi Zhao, Qurban Ali, Minghong Zheng, Zheng Tan, Hemin Yao, Shuqing Zheng, Jingni Wu, Jianhong Xu, Jianrong Shi, Huijun Wu, Xuewen Gao, Qin Gu
The plant rapid alkalinization factor (RALF) peptides function as key regulators in cell growth and immune responses through the receptor kinase FERONIA (FER). In this study, we report that the transcription factor FgPacC binds directly to the promoter of FgRALF gene, which encodes a functional homologue of the plant RALF peptides from the wheat head blight fungus Fusarium graminearum (FgRALF). More
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A natural variation in OsDSK2a modulates plant growth and salt tolerance through phosphorylation by SnRK1A in rice Plant Biotech. J. (IF 13.8) Pub Date : 2024-02-12 Juan Wang, Rui Zhu, Qingshi Meng, Hua Qin, Ruidang Quan, Pengcheng Wei, Xiaoying Li, Lei Jiang, Rongfeng Huang
Plants grow rapidly for maximal production under optimal conditions; however, they adopt a slower growth strategy to maintain survival when facing environmental stresses. As salt stress restricts crop architecture and grain yield, identifying genetic variations associated with growth and yield responses to salinity is critical for breeding optimal crop varieties. OsDSK2a is a pivotal modulator of plant
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Spatiotemporal transcriptomic atlas of rhizome formation in Oryza longistaminata Plant Biotech. J. (IF 13.8) Pub Date : 2024-02-12 Xiaoping Lian, Liyuan Zhong, Yixuan Bai, Xuanmin Guang, Sijia Tang, Xing Guo, Tong Wei, Feng Yang, Yujiao Zhang, Guangfu Huang, Jing Zhang, Lin Shao, Guijie Lei, Zheng Li, Sunil Kumar Sahu, Shilai Zhang, Huan Liu, Fengyi Hu
Rhizomes are modified stems that grow underground and produce new individuals genetically identical to the mother plant. Recently, a breakthrough has been made in efforts to convert annual grains into perennial ones by utilizing wild rhizomatous species as donors, yet the developmental biology of this organ is rarely studied. Oryza longistaminata, a wild rice species featuring strong rhizomes, provides
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The C4 photosynthesis bifunctional enzymes, PDRPs, of maize are co-opted to cytoplasmic viral replication complexes to promote infection of a prevalent potyvirus sugarcane mosaic virus Plant Biotech. J. (IF 13.8) Pub Date : 2024-02-10 Jipeng Xie, Xiaohong Fei, Qin Yan, Tong Jiang, Zhifang Li, Hui Chen, Baichen Wang, Qing Chao, Yueqiu He, Zaifeng Fan, Lijin Wang, Meng Wang, Liang Shi, Tao Zhou
In maize, two pyruvate orthophosphate dikinase (PPDK) regulatory proteins, ZmPDRP1 and ZmPDRP2, are respectively specific to the chloroplast of mesophyll cells (MCs) and bundle sheath cells (BSCs). Functionally, ZmPDRP1/2 catalyse both phosphorylation/inactivation and dephosphorylation/activation of ZmPPDK, which is implicated as a major rate-limiting enzyme in C4 photosynthesis of maize. Our study
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Calmodulin-like protein CML15 interacts with PP2C46/65 to regulate papaya fruit ripening via integrating calcium, ABA and ethylene signals Plant Biotech. J. (IF 13.8) Pub Date : 2024-02-06 Qiunan Zhu, Qinqin Tan, Qiyang Gao, Senlin Zheng, Weixin Chen, Jean-Philippe Galaud, Xueping Li, Xiaoyang Zhu
It is well known that calcium, ethylene and abscisic acid (ABA) can regulate fruit ripening, however, their interaction in the regulation of fruit ripening has not yet been fully clarified. The present study found that the expression of the papaya calcium sensor CpCML15 was strongly linked to fruit ripening. CpCML15 could bind Ca2+ and served as a true calcium sensor. CpCML15 interacted with CpPP2C46
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Efficient haploid induction via egg cell expression of dandelion PARTHENOGENESIS in foxtail millet (Setaria italica) Plant Biotech. J. (IF 13.8) Pub Date : 2024-02-06 Yong Huang, Yuqing Liang, Yingying Xie, Yuchun Rao, Jie Xiong, Chaolei Liu, Chun Wang, Xingchun Wang, Qian Qian, Kejian Wang
Foxtail millet (Setaria italica) is one of the most significant multigrain crops in arid and semi-arid regions worldwide. Given its relatively small genome size, strong environmental adaptability, high C4 light efficiency and remarkable nutritional value, foxtail millet is emerging as a rapidly developing C4 model crop for research on drought resistance, nutrition efficiency, and photosynthesis (Peng
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Homoeologous exchanges contribute to branch angle variations in rapeseed: Insights from transcriptome, QTL-seq and gene functional analysis Plant Biotech. J. (IF 13.8) Pub Date : 2024-02-03 Chengming Sun, Jian Wu, Xiaoying Zhou, Sanxiong Fu, Huimin Liu, Zhifei Xue, Xiaodong Wang, Qi Peng, Jianqin Gao, Feng Chen, Wei Zhang, Maolong Hu, Tingdong Fu, Youping Wang, Bin Yi, Jiefu Zhang
Branch angle (BA) is a critical morphological trait that significantly influences planting density, light interception and ultimately yield in plants. Despite its importance, the regulatory mechanism governing BA in rapeseed remains poorly understood. In this study, we generated 109 transcriptome data sets for 37 rapeseed accessions with divergent BA phenotypes. Relative to adaxial branch segments
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Novel CRISPR/Cas9 system assisted by fluorescence marker and pollen killer for high-efficiency isolation of transgene-free edited plants in rice Plant Biotech. J. (IF 13.8) Pub Date : 2024-02-01 Dong Yu, Tianshun Zhou, Na Xu, Xuewu Sun, Shufeng Song, Hai Liu, Zhizhong Sun, Qiming Lv, Jin Chen, Yanning Tan, Xiabing Sheng, Li Li, Dingyang Yuan
CRISPR/Cas9 gene-editing technology has revolutionized functional genomics and crop genetic improvement due to its simplicity, efficiency and stability (Gao, 2021). Nevertheless, the presence of CRISPR/Cas9 elements in the edited offspring increases the likelihood of inducing unstable and unpredictable phenotypes, posing challenges for commercial and research applications owing to the ongoing editing
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Unveiling the mechanism of broad-spectrum blast resistance in rice: The collaborative role of transcription factor OsGRAS30 and histone deacetylase OsHDAC1 Plant Biotech. J. (IF 13.8) Pub Date : 2024-01-31 Jiaqi Hou, Huangzhuo Xiao, Peng Yao, Xiaoci Ma, Qipeng Shi, Jin Yang, Haoli Hou, Lijia Li
Rice blast, caused by Magnaporthe oryzae, significantly impacts grain yield, necessitating the identification of broad-spectrum resistance genes and their functional mechanisms for disease-resistant crop breeding. Here, we report that rice with knockdown OsHDAC1 gene expression displays enhanced broad-spectrum blast resistance without effects on plant height and tiller numbers compared to wild-type
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Multiple strategies, including 6mA methylation, affecting plant alternative splicing in allopolyploid peanut Plant Biotech. J. (IF 13.8) Pub Date : 2024-01-31 Lihui Wang, Hua Chen, Yuhui Zhuang, Kun Chen, Chong Zhang, Tiecheng Cai, Qiang Yang, Huiwen Fu, Xiangyu Chen, Annapurna Chitkineni, Xiyin Wang, Rajeev K. Varshney, Weijian Zhuang
Alternative splicing (AS), an important post-transcriptional regulation mechanism in eukaryotes, can significantly increase transcript diversity and contribute to gene expression regulation and many other complicated developmental processes. While plant gene AS events are well described, few studies have investigated the comprehensive regulation machinery of plant AS. Here, we use multi-omics to analyse
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The chromosome-level assembly of the wild diploid alfalfa genome provides insights into the full landscape of genomic variations between cultivated and wild alfalfa Plant Biotech. J. (IF 13.8) Pub Date : 2024-01-30 Kun Shi, Hongbin Dong, Huilong Du, Yuxian Li, Le Zhou, Chengzhi Liang, Muhammet Şakiroğlu, Zan Wang
Alfalfa (Medicago sativa L.) is one of the most important forage legumes in the world, including autotetraploid (M. sativa ssp. sativa) and diploid alfalfa (M. sativa ssp. caerulea, progenitor of autotetraploid alfalfa). Here, we reported a high-quality genome of ZW0012 (diploid alfalfa, 769 Mb, contig N50 = 5.5 Mb), which was grouped into the Northern group in population structure analysis, suggesting
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MSH7 confers quantitative variation in pollen fertility and boosts grain yield in maize Plant Biotech. J. (IF 13.8) Pub Date : 2024-01-23 Luguang Jiang, Ting Guo, Xinyuan Song, Huan Jiang, Minhui Lu, Jinhong Luo, Vincenzo Rossi, Yan He
Fertile pollen is critical for the survival, fitness, and dispersal of flowering plants, and directly contributes to crop productivity. Extensive mutational screening studies have been carried out to dissect the genetic regulatory network determining pollen fertility, but we still lack fundamental knowledge about whether and how pollen fertility is controlled in natural populations. We used a genome-wide
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CsHLS1-CsSCL28 module regulates compact plant architecture in cucumber Plant Biotech. J. (IF 13.8) Pub Date : 2024-01-23 Chunhua Wang, Jie Li, Kai Fang, Hongxin Yao, Xingwen Chai, Yalin Du, Junwei Wang, Ning Hao, Jiajian Cao, Baohai Li, Tao Wu
Increased planting densities boost crop yields. A compact plant architecture facilitates dense planting. However, the mechanisms regulating compact plant architecture in cucurbits remain unclear. In this study, we identified a cucumber (Cucumis sativus) compact plant architecture (cpa1) mutant from an ethyl methane sulfonate (EMS)-mutagenized library that exhibited distinctive phenotypic traits, including
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The elite eating quality alleles Wxb and ALKb are regulated by OsDOF18 and coordinately improve head rice yield Plant Biotech. J. (IF 13.8) Pub Date : 2024-01-21 Maodi Zhu, Yongqiang Liu, Guiai Jiao, Junming Yu, Rumeng Zhao, Ao Lu, Wei Zhou, Ni Cao, Jiamin Wu, Shikai Hu, Zhonghua Sheng, Xiangjin Wei, Fengli Zhao, Lihong Xie, Shakeel Ahmad, Yongjun Lin, Gaoneng Shao, Shaoqing Tang, Peisong Hu
Head rice yield (HRY) measures rice milling quality and determines final grain yield and commercial value. Here, we report that two major quantitative trait loci for milling quality in rice, qMq-1 and qMq-2, represent allelic variants of Waxylv/Waxyb (hereafter Wx) encoding Granule-Bound Starch Synthase I (GBSSI) and Alkali Spreading Value ALKc/ALKb encoding Soluble Starch Synthase IIa (SSIIa), respectively
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Editing VvDXS1 for the creation of muscat flavour in Vitis vinifera cv. Scarlet Royal Plant Biotech. J. (IF 13.8) Pub Date : 2024-01-20 Yingzhen Yang, Matthew Wheatley, Victoria Meakem, Erin Galarneau, Benjamin Gutierrez, Gan-Yuan Zhong
Muscat flavour represents a group of unique aromatic attributes in some grape varieties. Biochemically, grape berries with muscat flavour produce high levels of monoterpenes. Monoterpene biosynthesis is mainly through the DOXP/MEP pathway, and VvDXS1 encodes the first enzyme in this plastidial pathway of terpene biosynthesis in grapevine. A single-point mutation resulting in the substitution of a lysine
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Novel synthetic inducible promoters controlling gene expression during water-deficit stress with green tissue specificity in transgenic poplar Plant Biotech. J. (IF 13.8) Pub Date : 2024-01-17 Yongil Yang, Timothy A. Chaffin, Yuanhua Shao, Vimal K. Balasubramanian, Meng Markillie, Hugh Mitchell, Maria M. Rubio-Wilhelmi, Amir H. Ahkami, Eduardo Blumwald, C. Neal Stewart
Synthetic promoters may be designed using short cis-regulatory elements (CREs) and core promoter sequences for specific purposes. We identified novel conserved DNA motifs from the promoter sequences of leaf palisade and vascular cell type-specific expressed genes in water-deficit stressed poplar (Populus tremula × Populus alba), collected through low-input RNA-seq analysis using laser capture microdissection
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Versatile CYP98A enzymes catalyse meta-hydroxylation reveals diversity of salvianolic acids biosynthesis Plant Biotech. J. (IF 13.8) Pub Date : 2024-01-16 Zheng Zhou, Jingxian Feng, Juncheng Huo, Shi Qiu, Pan Zhang, Yun Wang, Qing Li, Yajing Li, Cuicui Han, Xiaobing Feng, Yonghao Duan, Ruibin Chen, Ying Xiao, Ying He, Lei Zhang, Wansheng Chen
Salvianolic acids (SA), such as rosmarinic acid (RA), danshensu (DSS), and their derivative salvianolic acid B (SAB), etc. widely existed in Lamiaceae and Boraginaceae families, are of interest due to medicinal properties in the pharmaceutical industries. Hundreds of studies in past decades described that 4-coumaroyl-CoA and 4-hydroxyphenyllactic acid (4-HPL) are common substrates to biosynthesize
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BnSTINet: An experimentally-based transcription factor interaction network in seeds of Brassica napus Plant Biotech. J. (IF 13.8) Pub Date : 2024-01-12 Yongtai Yin, Jia Jia, Hongsheng He, Weiguo Zhao, Zhenyi Guo, Kang Chen, Huaixin Li, Jianjie He, Yiran Ding, Wang Chen, Jingrong Li, Yujiao Li, Haikun Zhang, Zilong Li, Nadia Raboanatahiry, Chunhua Fu, Libin Zhang, Longjiang Yu, Maoteng Li
For the past decade, research on constructing large-scale plant protein–protein interaction (PPI) networks has mainly focused on model plants, and the capability of experimental-based large-scale PPI networks in these plants was thought to be significantly inferior to the actual number of protein interactions in plants (Altmann et al., 2020; Jones et al., 2014; Smakowska-Luzan et al., 2018; Wang et al
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Plant microphenotype: from innovative imaging to computational analysis Plant Biotech. J. (IF 13.8) Pub Date : 2024-01-13 Ying Zhang, Shenghao Gu, Jianjun Du, Guanmin Huang, Jiawei Shi, Xianju Lu, Jinglu Wang, Wanneng Yang, Xinyu Guo, Chunjiang Zhao
The microphenotype plays a key role in bridging the gap between the genotype and the complex macro phenotype. In this article, we review the advances in data acquisition and the intelligent analysis of plant microphenotyping and present applications of microphenotyping in plant science over the past two decades. We then point out several challenges in this field and suggest that cross-scale image acquisition
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Genomic basis of seed colour in quinoa inferred from variant patterns using extreme gradient boosting Plant Biotech. J. (IF 13.8) Pub Date : 2024-01-11 Felix L. Sandell, Thomas Holzweber, Nathaniel R. Street, Juliane C. Dohm, Heinz Himmelbauer
Quinoa is an agriculturally important crop species originally domesticated in the Andes of central South America. One of its most important phenotypic traits is seed colour. Seed colour variation is determined by contrasting abundance of betalains, a class of strong antioxidant and free radicals scavenging colour pigments only found in plants of the order Caryophyllales. However, the genetic basis
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Editing of ORF138 restores fertility of Ogura cytoplasmic male sterile broccoli via mitoTALENs Plant Biotech. J. (IF 13.8) Pub Date : 2024-01-11 Fengyuan Xu, Tongbing Su, Xiaochen Zhang, Lei Qiu, Xiaodong Yang, Nobuya Koizuka, Shin-ichi Arimura, Zhongyuan Hu, Mingfang Zhang, Jinghua Yang
Cytoplasmic male sterility (CMS), encoded by the mitochondrial open reading frames (ORFs), has long been used to economically produce crop hybrids. However, the utilization of CMS also hinders the exploitation of sterility and fertility variation in the absence of a restorer line, which in turn narrows the genetic background and reduces biodiversity. Here, we used a mitochondrial targeted transcription
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Glucose uptake from the rhizosphere mediated by MdDOF3-MdHT1.2 regulates drought resistance in apple Plant Biotech. J. (IF 13.8) Pub Date : 2024-01-11 Xiaocheng Tian, Yuxing Li, Shaoteng Wang, Hui Zou, Qian Xiao, Baiquan Ma, Fengwang Ma, Mingjun Li
In plants under drought stress, sugar content in roots increases, which is important for drought resistance. However, the molecular mechanisms for controlling the sugar content in roots during response to drought remain elusive. Here, we found that the MdDOF3-MdHT1.2 module-mediated glucose influx into the root is essential for drought resistance in apple (Malus × domestica). Drought induced glucose
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Celebrating Professor Rajeev K. Varshney's transformative research odyssey from genomics to the field on his induction as Fellow of the Royal Society Plant Biotech. J. (IF 13.8) Pub Date : 2024-01-11 Vanika Garg, Rutwik Barmukh, Annapurna Chitikineni, Manish Roorkiwal, Chris Ojiewo, Abhishek Bohra, Mahendar Thudi, Vikas K. Singh, Himabindu Kudapa, Rachit K. Saxena, Jake Fountain, Reyazul Rouf Mir, Chellapilla Bharadwaj, Xiaoping Chen, Liu Xin, Manish K. Pandey
Professor Rajeev K. Varshney's transformative impact on crop genomics, genetics, and agriculture is the result of his passion, dedication, and unyielding commitment to harnessing the potential of genomics to address the most pressing challenges faced by the global agricultural community. Starting from a small town in India and reaching the global stage, Professor Varshney's academic and professional
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A newly evolved rice-specific gene JAUP1 regulates jasmonate biosynthesis and signalling to promote root development and multi-stress tolerance Plant Biotech. J. (IF 13.8) Pub Date : 2024-01-09 Adnan Muzaffar, Yi-Shih Chen, Hsiang-Ting Lee, Cheng-Chieh Wu, Trang Thi Le, Jin-Zhang Liang, Chun-Hsien Lu, Hariharan Balasubramaniam, Shuen-Fang Lo, Lin-Chih Yu, Chien-Hao Chan, Ku-Ting Chen, Miin-Huey Lee, Yue-Ie Hsing, Tuan-Hua David Ho, Su-May Yu
Root architecture and function are critical for plants to secure water and nutrient supply from the soil, but environmental stresses alter root development. The phytohormone jasmonic acid (JA) regulates plant growth and responses to wounding and other stresses, but its role in root development for adaptation to environmental challenges had not been well investigated. We discovered a novel JA Upregulated
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Metabolite-based genome-wide association studies enable the dissection of the genetic bases of flavonoids, betaine and spermidine in wolfberry (Lycium) Plant Biotech. J. (IF 13.8) Pub Date : 2024-01-09 Jianhua Zhao, Yuhui Xu, Haoxia Li, Wei An, Yue Yin, Bin Wang, Liping Wang, Bi Wang, Linyuan Duan, Xiaoyue Ren, Xiaojie Liang, Yajun Wang, Ru Wan, Ting Huang, Bo Zhang, Yanlong Li, Jie Luo, Youlong Cao
Wolfberry is a plant with medicinal and food values. However, its bioactive ingredients and the corresponding genetic bases have not been determined. Here, we de novo generated a chromosome-level genome assembly for wolfberry, yielding a genome sequence of ~1.77 Gb with contig N50 of 50.55 Mb and 39 224 predicted gene models. A variation map, using 307 re-sequenced accessions, was called based on this
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Wearable sensor supports in-situ and continuous monitoring of plant health in precision agriculture era Plant Biotech. J. (IF 13.8) Pub Date : 2024-01-06 Xiao-Hong Li, Meng-Zhao Li, Jing-Yi Li, Yang-Yang Gao, Chun-Rong Liu, Ge-Fei Hao
Plant health is intricately linked to crop quality, food security and agricultural productivity. Obtaining accurate plant health information is of paramount importance in the realm of precision agriculture. Wearable sensors offer an exceptional avenue for investigating plant health status and fundamental plant science, as they enable real-time and continuous in-situ monitoring of physiological biomarkers