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Targeted knockout of early nodulin-like 3 (MusaENODL3) gene in banana reveals its function in resistance to Xanthomonas wilt disease Plant Biotech. J. (IF 13.8) Pub Date : 2023-11-28 Valentine Otang Ntui, Jaindra Nath Tripathi, Trushar Shah, Leena Tripathi
Nodulins and nodulin-like proteins play an essential role in the symbiotic associations between legumes and Rhizobium bacteria. Their role extends beyond the leguminous species, as numerous nodulin-like proteins, including early nodulin-like proteins (ENODL), have been identified in various non-leguminous plants, implying their involvement in functions beyond nodulation, such as nutrient transport
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ZmELF6-ZmPRR37 module regulates maize flowering and salt response Plant Biotech. J. (IF 13.8) Pub Date : 2023-11-27 Huihui Su, Liru Cao, Zhenzhen Ren, Wenhao Sun, Bingqi Zhu, Shixiang Ma, Chongyu Sun, Dongling Zhang, Zhixue Liu, Haixia Zeng, Wenjing Yang, Yingpeng Liu, Lingling Zheng, Yuwei Yang, Zhendong Wu, Yingfang Zhu, Lixia Ku, Leelyn Chong, Yanhui Chen
The control of flowering time in maize is crucial for reproductive success and yield, and it can be influenced by environmental stresses. Using the approaches of Ac/Ds transposon and transposable element amplicon sequencing techniques, we identified a Ds insertion mutant in the ZmPRR37 gene. The Ds insertion showed a significant correlation with days to anthesis. Further research indicated that ZmPRR37-CR
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A dual-subcellular localized β-glucosidase confers pathogen and insect resistance without a yield penalty in maize Plant Biotech. J. (IF 13.8) Pub Date : 2023-11-27 Chuang Liu, Shengfeng He, Junbin Chen, Mingyu Wang, Zhenju Li, Luyang Wei, Yan Chen, Meida Du, Dandan Liu, Cai Li, Chunju An, Vijai Bhadauria, Jinsheng Lai, Wangsheng Zhu
Maize is one of the most important crops for food, cattle feed and energy production. However, maize is frequently attacked by various pathogens and pests, which pose a significant threat to maize yield and quality. Identification of quantitative trait loci and genes for resistance to pests will provide the basis for resistance breeding in maize. Here, a β-glucosidase ZmBGLU17 was identified as a resistance
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Development of TALE-adenine base editors in plants Plant Biotech. J. (IF 13.8) Pub Date : 2023-11-24 Dingbo Zhang, Jens Boch
Base editors enable precise nucleotide changes at targeted genomic loci without requiring double-stranded DNA breaks or repair templates. TALE-adenine base editors (TALE-ABEs) are genome editing tools, composed of a DNA-binding domain from transcription activator-like effectors (TALEs), an engineered adenosine deaminase (TadA8e), and a cytosine deaminase domain (DddA), that allow A•T-to-G•C editing
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Elevating plant immunity by translational regulation of a rice WRKY transcription factor Plant Biotech. J. (IF 13.8) Pub Date : 2023-11-23 Chao Zheng, Jie Zhou, Xiaoya Yuan, Ersong Zheng, Xiuli Liu, Weijun Cui, Chengqi Yan, Yueyan Wu, Wenyuan Ruan, Keke Yi, Jianping Chen, Xuming Wang
Plants have intricate mechanisms that tailor their defence responses to pathogens. WRKY transcription factors play a pivotal role in plant immunity by regulating various defence signalling pathways. Many WRKY genes are transcriptionally activated upon pathogen attack, but how their functions are regulated after transcription remains elusive. Here, we show that OsWRKY7 functions as a crucial positive
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Genome editing of a dominant resistance gene for broad-spectrum resistance to bacterial diseases in rice without growth penalty Plant Biotech. J. (IF 13.8) Pub Date : 2023-11-23 Meixia Wang, Shaofang Li, Huayang Li, Congfeng Song, Wenya Xie, Shimin Zuo, Xueping Zhou, Changyong Zhou, Zhiyuan Ji, Huanbin Zhou
Bacterial blight (BB) and bacterial leaf streak (BLS), caused by Xanthomonas oryzae pv. oryzae (Xoo) and Xanthomonas oryzae pv. oryzicola (Xoc), respectively, are the two most devastating bacterial diseases of rice worldwide. Both bacterial pathogens infect rice plants relying on type III secreted transcriptional activation-like effectors (TALEs) that bind to specific effector binding elements (EBEs)
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Shaping rice Green Revolution traits by engineering ATG immediate upstream 5′-UTR sequences of OsSBI and OsHTD1 Plant Biotech. J. (IF 13.8) Pub Date : 2023-11-23 Hongwen Wang, Mingjiang Chen, Dahan Zhang, Xiangbing Meng, Jijun Yan, Jinfang Chu, Jiayang Li, Hong Yu
During the rice ‘Green Revolution’, the semidwarf phenotype for lodging resistance and the high-tillering trait for high yield were co-selected by pyramiding semi-dwarf 1 (SD1) and high tillering and dwarf 1/carotenoid cleavage dioxygenase 7 (HTD1/CCD7) from Dee-geo-woo-gen and Peta, two parents of the ‘miracle rice’ IR8 (Asano et al., 2011; Wang et al., 2020). Recent advances in genome editing have
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Editing the 19 kDa alpha-zein gene family generates non-opaque2-based quality protein maize Plant Biotech. J. (IF 13.8) Pub Date : 2023-11-21 J. Preston Hurst, Shirley Sato, Tyler Ferris, Abou Yobi, You Zhou, Ruthie Angelovici, Tom E. Clemente, David R. Holding
Maize grain is deficient in lysine. While the opaque2 mutation increases grain lysine, o2 is a transcription factor that regulates a wide network of genes beyond zeins, which leads to pleiotropic and often negative effects. Additionally, the drastic reduction in 19 kDa and 22 kDa alpha-zeins causes a floury kernel, unsuitable for agricultural use. Quality protein maize (QPM) overcame the undesirable
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Generating herbicide resistant and dwarf rice germplasms through precise sequence insertion or replacement Plant Biotech. J. (IF 13.8) Pub Date : 2023-11-22 Xiaoxia Liu, Yingying Wang, Hongzhi Wang, Yuxin He, Yijiao Song, Zuren Li, Ming Li, Chuang Wei, Yinghui Dong, Li Xue, Jinshan Zhang, Jian-Kang Zhu, Mugui Wang
Precise sequence insertion or replacement in plants is technically challenging but is of great importance in crop breeding because many agronomic traits are affected by DNA fragment variations. Although prime editing (PE) has been continuously optimized to improve its activity in plants (Jiang et al., 2022; Li et al., 2022a,b; Zong et al., 2022), it is still inefficient for targeted insertion or replacement
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Natural allelic diversities of GmPrx16 confer drought tolerance in soybean Plant Biotech. J. (IF 13.8) Pub Date : 2023-11-22 Zhifang Zhang, Junkui Ma, Xia Yang, Zhi Liu, Yucheng Liu, Xueyi Liu, Shan Liang, Zongbiao Duan, Zheng Wang, Xiaoyue Yang, Long Yan, Min Zhang, Shulin Liu, Zhixi Tian
Drought has a serious impact on agricultural production, which can result in production losses ranging between 30% and 90%, depending on the crop species (Hussain et al., 2019; Lesk et al., 2016). Soybean [Glycine max (L.) Merr.] is an important crop that provides primary vegetable oil and protein as well as a supplement in livestock feed worldwide (Wilson, 2008). However, soybean is very sensitive
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Allelic variations of WAK106-E2Fa-DPb1-UGT74E2 module regulate fibre properties in Populus tomentosa Plant Biotech. J. (IF 13.8) Pub Date : 2023-11-21 Dan Wang, Mingyang Quan, Shitong Qin, Yuanyuan Fang, Liang Xiao, Weina Qi, Yongsen Jiang, Jiaxuan Zhou, Mingyue Gu, Yicen Guan, Qingzhang Du, Qing Liu, Yousry A. El-Kassaby, Deqiang Zhang
Wood formation, intricately linked to the carbohydrate metabolism pathway, underpins the capacity of trees to produce renewable resources and offer vital ecosystem services. Despite their importance, the genetic regulatory mechanisms governing wood fibre properties in woody plants remain enigmatic. In this study, we identified a pivotal module comprising 158 high-priority core genes implicated in wood
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Uncovering epigenetic and transcriptional regulation of growth in Douglas-fir: identification of differential methylation regions in mega-sized introns Plant Biotech. J. (IF 13.8) Pub Date : 2023-11-20 Giang Thi Ha Vu, Hieu Xuan Cao, Martin Hofmann, Wilfried Steiner, Oliver Gailing
Tree growth performance can be partly explained by genetics, while a large proportion of growth variation is thought to be controlled by environmental factors. However, to what extent DNA methylation, a stable epigenetic modification, contributes to phenotypic plasticity in the growth performance of long-lived trees remains unclear. In this study, a comparative analysis of targeted DNA genotyping,
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GW9 determines grain size and floral organ identity in rice Plant Biotech. J. (IF 13.8) Pub Date : 2023-11-20 Yi Wen, Peng Hu, Yunxia Fang, Yiqing Tan, Yueying Wang, Hao Wu, Junge Wang, Kaixiong Wu, Bingze Chai, Li Zhu, Guangheng Zhang, Zhenyu Gao, Deyong Ren, Dali Zeng, Lan Shen, Guojun Dong, Qiang Zhang, Qing Li, Guosheng Xiong, Dawei Xue, Qian Qian, Jiang Hu
Grain weight is an important determinant of grain yield. However, the underlying regulatory mechanisms for grain size remain to be fully elucidated. Here, we identify a rice mutant grain weight 9 (gw9), which exhibits larger and heavier grains due to excessive cell proliferation and expansion in spikelet hull. GW9 encodes a nucleus-localized protein containing both C2H2 zinc finger (C2H2-ZnF) and
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CRISPR-based editing of the ω- and γ-gliadin gene clusters reduces wheat immunoreactivity without affecting grain protein quality Plant Biotech. J. (IF 13.8) Pub Date : 2023-11-17 Zitong Yu, Ural Yunusbaev, Allan Fritz, Michael Tilley, Alina Akhunova, Harold Trick, Eduard Akhunov
Wheat immunotoxicity is associated with abnormal reaction to gluten-derived peptides. Attempts to reduce immunotoxicity using breeding and biotechnology often affect dough quality. Here, the multiplexed CRISPR-Cas9 editing of cultivar Fielder was used to modify gluten-encoding genes, specifically focusing on ω- and γ-gliadin gene copies, which were identified to be abundant in immunoreactive peptides
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MicroRNA482/2118 is lineage-specifically involved in gibberellin signalling via the regulation of GID1 expression by targeting noncoding PHAS genes and subsequently instigated phasiRNAs Plant Biotech. J. (IF 13.8) Pub Date : 2023-11-15 Yanqing Zhang, Zaohai Zeng, Huimin Hu, Minglei Zhao, Chengjie Chen, Xingshuai Ma, Guanliang Li, Jianguo Li, Yuanlong Liu, Yanwei Hao, Jing Xu, Rui Xia
MicroRNA482/2118 (miR482/2118) is a 22-nt miRNA superfamily, with conserved functions in disease resistance and plant development. It usually instigates the production of phased small interfering RNAs (phasiRNAs) from its targets to expand or reinforce its silencing effect. Using a new high-quality reference genome sequence and comprehensive small RNA profiling, we characterized a newly evolved regulatory
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Plant virus-derived nanoparticles decorated with genetically encoded SARS-CoV-2 nanobodies display enhanced neutralizing activity Plant Biotech. J. (IF 13.8) Pub Date : 2023-11-15 Fernando Merwaiss, Enrique Lozano-Sanchez, João Zulaica, Luciana Rusu, Marta Vazquez-Vilar, Diego Orzáez, Guillermo Rodrigo, Ron Geller, José-Antonio Daròs
Viral nanoparticles (VNPs) are a new class of virus-based formulations that can be used as building blocks to implement a variety of functions of potential interest in biotechnology and nanomedicine. Viral coat proteins (CP) that exhibit self-assembly properties are particularly appropriate for displaying antigens and antibodies, by generating multivalent VNPs with therapeutic and diagnostic potential
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Overexpression of OsACL5 triggers environmentally-dependent leaf rolling and reduces grain size in rice Plant Biotech. J. (IF 13.8) Pub Date : 2023-11-15 Huafu Mai, Tian Qin, Huan Wei, Zhen Yu, Gang Pang, Zhiman Liang, Jiansheng Ni, Haishan Yang, Haiying Tang, Lisi Xiao, Huili Liu, Taibo Liu
Major polyamines include putrescine, spermidine, spermine and thermospermine, which play vital roles in growth and adaptation against environmental changes in plants. Thermospermine (T-Spm) is synthetised by ACL5. The function of ACL5 in rice is still unknown. In this study, we used a reverse genetic strategy to investigate the biological function of OsACL5. We generated several knockout mutants by
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Unravelling inversions: Technological advances, challenges, and potential impact on crop breeding Plant Biotech. J. (IF 13.8) Pub Date : 2023-11-14 Haifei Hu, Armin Scheben, Jian Wang, Fangping Li, Chengdao Li, David Edwards, Junliang Zhao
Inversions, a type of chromosomal structural variation, significantly influence plant adaptation and gene functions by impacting gene expression and recombination rates. However, compared with other structural variations, their roles in functional biology and crop improvement remain largely unexplored. In this review, we highlight technological and methodological advancements that have allowed a comprehensive
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CRISPR/Cas9-mediated mutations of FANTASTIC FOUR gene family for creating early flowering mutants in tomato Plant Biotech. J. (IF 13.8) Pub Date : 2023-11-09 Lele Shang, Jinbao Tao, Jianwen Song, Yaru Wang, Xingyu Zhang, Pingfei Ge, Fangman Li, Haiqiang Dong, Wenxian Gai, Donald Grierson, Zhibiao Ye, Yuyang Zhang
Flowering time is of great agricultural importance and the timing and extent of flowering usually determines yield and availability of flowers, fruits and seeds. Identification of genes determining flowering has important practical applications for tomato breeding. Here we demonstrate the roles of the FANTASTIC FOUR (FAF) gene family in regulating tomato flowering time. In this plant-specific gene
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The TaSOC1-TaVRN1 module integrates photoperiod and vernalization signals to regulate wheat flowering Plant Biotech. J. (IF 13.8) Pub Date : 2023-11-08 Xumei Luo, Bingyan Liu, Li Xie, Ke Wang, Dengan Xu, Xiuling Tian, Lina Xie, Lingli Li, Xingguo Ye, Zhonghu He, Xianchun Xia, Liuling Yan, Shuanghe Cao
Wheat needs different durations of vernalization, which accelerates flowering by exposure to cold temperature, to ensure reproductive development at the optimum time, as that is critical for adaptability and high yield. TaVRN1 is the central flowering regulator in the vernalization pathway and encodes a MADS-box transcription factor (TF) that usually works by forming hetero- or homo-dimers. We previously
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Natural variation in Fatty Acid 9 is a determinant of fatty acid and protein content Plant Biotech. J. (IF 13.8) Pub Date : 2023-11-08 Zhaoming Qi, Chaocheng Guo, Haiyang Li, Hongmei Qiu, Hui Li, CholNam Jong, Guolong Yu, Yu Zhang, Limin Hu, Xiaoxia Wu, Dawei Xin, Mingliang Yang, Chunyan Liu, Jian Lv, Xu Wang, Fanjiang Kong, Qingshan Chen
Soybean is one of the most economically important crops worldwide and an important source of unsaturated fatty acids and protein for the human diet. Consumer demand for healthy fats and oils is increasing, and the global demand for vegetable oil is expected to double by 2050. Identification of key genes that regulate seed fatty acid content can facilitate molecular breeding of high-quality soybean
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Fine-tuning rice heading date through multiplex editing of the regulatory regions of key genes by CRISPR-Cas9 Plant Biotech. J. (IF 13.8) Pub Date : 2023-11-06 Shirong Zhou, Liang Cai, Haoqin Wu, Baoxiang Wang, Biao Gu, Song Cui, Xiaolong Huang, Zhuang Xu, Benyuan Hao, Haigang Hou, Yuan Hu, Chao Li, Yunlu Tian, Xi Liu, Liangming Chen, Shijia Liu, Ling Jiang, Jianmin Wan
Heading date (or flowering time) is a key agronomic trait that affects seasonal and regional adaption of rice cultivars. An unoptimized heading date can either not achieve a high yield or has a high risk of encountering abiotic stresses. There is a strong demand on the mild to moderate adjusting the heading date in breeding practice. Genome editing is a promising method which allows more precise and
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Blocking miR528 function promotes tillering and regrowth in switchgrass Plant Biotech. J. (IF 13.8) Pub Date : 2023-11-06 Xiangyan Han, Shanjie Tang, Xuan Ma, Wenwen Liu, Ruijuan Yang, Shuaibin Zhang, Ningning Wang, Xianwei Song, Chunxiang Fu, Rongxin Yang, Xiaofeng Cao
MiRNAs have been reported to be the key regulators involving a wide range of biological processes in diverse plant species, but their functions in switchgrass, an important biofuel and forage crop, are largely unknown. Here, we reported the novel function of miR528, which has expanded to four copies in switchgrass, in controlling biomass trait of tillering number and regrowth rate after mowing. Blocking
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EARLY FLOWERING is a dominant gain-of-function allele of FANTASTIC FOUR 1/2c that promotes early flowering in tomato Plant Biotech. J. (IF 13.8) Pub Date : 2023-11-06 Dedi Zhang, Guo Ai, Kangna Ji, Rong Huang, Chunrui Chen, Zixuan Yang, Jiafa Wang, Long Cui, Guobin Li, Maryam Tahira, Xin Wang, Taotao Wang, Jie Ye, Zonglie Hong, Zhibiao Ye, Junhong Zhang
Flowering time, an important factor in plant adaptability and genetic improvement, is regulated by various genes in tomato (Solanum lycopersicum). In this study, we characterized a tomato mutant, EARLY FLOWERING (EF), that developed flowers much earlier than its parental control. EF is a dominant gain-of-function allele with a T-DNA inserted 139 bp downstream of the stop codon of FANTASTIC FOUR 1/2c
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Genomics of predictive radiation mutagenesis in oilseed rape: modifying seed oil composition Plant Biotech. J. (IF 13.8) Pub Date : 2023-11-03 Lenka Havlickova, Zhesi He, Madeleine Berger, Lihong Wang, Greta Sandmann, Yen Peng Chew, Guilherme V. Yoshikawa, Guangyuan Lu, Qiong Hu, Surinder S. Banga, Frederic Beaudoin, Ian Bancroft
Rapeseed is a crop of global importance but there is a need to broaden the genetic diversity available to address breeding objectives. Radiation mutagenesis, supported by genomics, has the potential to supersede genome editing for both gene knockout and copy number increase, but detailed knowledge of the molecular outcomes of radiation treatment is lacking. To address this, we produced a genome re-sequenced
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The transcriptional regulatory module CsHB5-CsbZIP44 positively regulates abscisic acid-mediated carotenoid biosynthesis in citrus (Citrus spp.) Plant Biotech. J. (IF 13.8) Pub Date : 2023-11-01 Quan Sun, Zhengchen He, Ranran Wei, Yin Zhang, Junli Ye, Lijun Chai, Zongzhou Xie, Wenwu Guo, Juan Xu, Yunjiang Cheng, Qiang Xu, Xiuxin Deng
Carotenoids contribute to fruit coloration and are valuable sources of provitamin A in the human diet. Abscisic acid (ABA) plays an essential role in fruit coloration during citrus fruit ripening, but little is known about the underlying mechanisms. Here, we identified a novel bZIP transcription activator called CsbZIP44, which serves as a central regulator of ABA-mediated citrus carotenoid biosynthesis
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Upland rice genomic signatures of adaptation to drought resistance and navigation to molecular design breeding Plant Biotech. J. (IF 13.8) Pub Date : 2023-11-01 Yulong Wang, Conghui Jiang, Xingtan Zhang, Huimin Yan, Zhigang Yin, Xingming Sun, Fenghua Gao, Yan Zhao, Wei Liu, Shichen Han, Jingjing Zhang, Yage Zhang, Zhanying Zhang, Hongliang Zhang, Jinjie Li, Xianzhi Xie, Quanzhi Zhao, Xiaoning Wang, Guoyou Ye, Junzhou Li, Ray Ming, Zichao Li
Upland rice is a distinctive drought-aerobic ecotype of cultivated rice highly resistant to drought stress. However, the genetic and genomic basis for the drought-aerobic adaptation of upland rice remains largely unclear due to the lack of genomic resources. In this study, we identified 25 typical upland rice accessions and assembled a high-quality genome of one of the typical upland rice varieties
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Origin and evolution of the kiwifruit Y chromosome Plant Biotech. J. (IF 13.8) Pub Date : 2023-10-31 Junyang Yue, Qinyao Chen, Sijia Zhang, Yunzhi Lin, Wangmei Ren, Bingjie Li, Ying Wu, Yingzhen Wang, Yongfeng Zhou, Yongsheng Liu
Actinidia, often known as kiwifruit, is functionally dioecious with separate female and male individuals. The sex determination of kiwifruit is genetically controlled by an XY system, even independent of ploidy levels. Recently, two Y-linked sex-determining genes, Shy Girl (SyGl) and Friendly Boy (FrBy), have been successively identified and characterized in A. chinensis (Akagi et al., 2018; Akagi
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Gene pyramiding for boosted plant growth and broad abiotic stress tolerance Plant Biotech. J. (IF 13.8) Pub Date : 2023-10-30 Guiqin Zhao, Yu Liu, Lei Li, Rui Che, Megan Douglass, Katherine Benza, Mitchell Angove, Kristopher Luo, Qian Hu, Xiaotong Chen, Charles Henry, Zhigang Li, Guogui Ning, Hong Luo
Abiotic stresses such as salinity, heat and drought seriously impair plant growth and development, causing a significant loss in crop yield and ornamental value. Biotechnology approaches manipulating specific genes prove to be effective strategies in crop trait modification. The Arabidopsis vacuolar pyrophosphatase gene AVP1, the rice SUMO E3 ligase gene OsSIZ1 and the cyanobacterium flavodoxin gene
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Increasing flavonoid contents of tomato fruits through disruption of the SlSPL-CNR, a suppressor of SlMYB12 transcription activity Plant Biotech. J. (IF 13.8) Pub Date : 2023-10-30 Leilei Zhou, Zongyan Sun, Tingting Hu, Di Chen, Xue Chen, Qiaoli Zhang, Jiankang Cao, Benzhong Zhu, Daqi Fu, Hongliang Zhu, Guiqin Qu
Fruits of tomato (Solanum lycopersicum) are rich in nutrients and generally served as critical resources for human diet. In fruit pericarps, the peel tissues adhering to the flesh produce functional components including flavonoids that are indeed mixed polyphenolic compounds. Flavonoids play an essential role in determining peel colour and are proven to be functionally important for human health as
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Enhanced late blight resistance by engineering an EpiC2B-insensitive immune protease Plant Biotech. J. (IF 13.8) Pub Date : 2023-10-30 Mariana Schuster, Sophie Eisele, Liz Armas-Egas, Till Kessenbrock, Jiorgos Kourelis, Markus Kaiser, Renier A. L. van der Hoorn
Papain-like immune proteases (PLCPs) are promising engineering targets for crop protection, given their significant roles in plant immunity for key crops such as tomato, maize and citrus (Misas-Villamil et al., 2016). The wide range of pathogen-secreted PLCP inhibitors highlights the importance of these proteases in defending against various pathogens. Depletion of the apoplastic immune PLCP Phytophthora-inhibited
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Soybean-Phakopsora pachyrhizi interactions: towards the development of next-generation disease-resistant plants Plant Biotech. J. (IF 13.8) Pub Date : 2023-10-26 Aline Sartor Chicowski, Melissa Bredow, Alice Satiko Utiyama, Francismar Corrêa Marcelino-Guimarães, Steven A. Whitham
Soybean rust (SBR), caused by the obligate biotrophic fungus Phakopsora pachyrhizi, is a devastating foliar disease threatening soybean production. To date, no commercial cultivars conferring durable resistance to SBR are available. The development of long-lasting SBR resistance has been hindered by the lack of understanding of this complex pathosystem, encompassing challenges posed by intricate genetic
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The elicitor VP2 from Verticillium dahliae triggers defence response in cotton Plant Biotech. J. (IF 13.8) Pub Date : 2023-10-26 Ping Qiu, Baoxin Zheng, Hang Yuan, Zhaoguang Yang, Keith Lindsey, Yan Wang, Yuqing Ming, Lin Zhang, Qin Hu, Muhammad Shaban, Jie Kong, Xianlong Zhang, Longfu Zhu
Verticillium dahliae is a widespread and destructive soilborne vascular pathogenic fungus that causes serious diseases in dicot plants. Here, comparative transcriptome analysis showed that the number of genes upregulated in defoliating pathotype V991 was significantly higher than in the non-defoliating pathotype 1cd3-2 during the early response of cotton. Combined with analysis of the secretome during
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A quantitative autonomous bioluminescence reporter system with a wide dynamic range for Plant Synthetic Biology Plant Biotech. J. (IF 13.8) Pub Date : 2023-10-26 Camilo Calvache, Marta Vazquez-Vilar, Elena Moreno-Giménez, Diego Orzaez
Plant Synthetic Biology aims to enhance the capacities of plants by designing and integrating synthetic gene circuits (SGCs). Quantitative reporting solutions that can produce quick, rich datasets affordably are necessary for SGC optimization. In this paper, we present a new, low-cost, and high-throughput reporter system for the quantitative measurement of gene expression in plants based on autonomous
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Differential heat-response characteristics of two plastid isoforms of triose phosphate isomerase in tomato Plant Biotech. J. (IF 13.8) Pub Date : 2023-10-25 Chong Chen, Meng Zhang, Xiaocui Ma, Qingwei Meng, Kunyang Zhuang
Heat stress causes dysfunction of the carbon-assimilation metabolism. As a member of Calvin–Benson–Bassham (CBB) cycle, the chloroplast triose phosphate isomerases (TPI) catalyse the interconversion of glyceraldehyde 3-phosphate (GAP) and dihydroxyacetone phosphate (DHAP). The tomato (Solanum lycopersicum) genome contains two individual SlTPI genes, Solyc10g054870 and Solyc01g111120, which encode the
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The chromatin remodeller MdRAD5B enhances drought tolerance by coupling MdLHP1-mediated H3K27me3 in apple Plant Biotech. J. (IF 13.8) Pub Date : 2023-10-24 Yi Song, Jieqiang He, Junxing Guo, Yinpeng Xie, Ziqing Ma, Zeyuan Liu, Chundong Niu, Xuewei Li, Baohua Chu, Muhammad Mobeen Tahir, Jidi Xu, Fengwang Ma, Qingmei Guan
RAD5B belongs to the Rad5/16-like group of the SNF2 family, which often functions in chromatin remodelling. However, whether RAD5B is involved in chromatin remodelling, histone modification, and drought stress tolerance is largely unclear. We identified a drought-inducible chromatin remodeler, MdRAD5B, which positively regulates apple drought tolerance. Transposase-accessible chromatin with high-throughput
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An ancient cis-element targeted by Ralstonia solanacearum TALE-like effectors facilitates the development of a promoter trap that could confer broad-spectrum wilt resistance Plant Biotech. J. (IF 13.8) Pub Date : 2023-10-23 Niels Gallas, Xiaoxu Li, Edda von Roepenack-Lahaye, Niklas Schandry, Yuxin Jiang, Dousheng Wu, Thomas Lahaye
Ralstonia solanacearum, a species complex of bacterial plant pathogens that causes bacterial wilt, comprises four phylotypes that evolved when a founder population was split during the continental drift ~180 million years ago. Each phylotype contains strains with RipTAL proteins structurally related to transcription activator-like (TAL) effectors from the bacterial pathogen Xanthomonas. RipTALs have
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The first two chromosome-scale genome assemblies of American hazelnut enable comparative genomic analysis of the genus Corylus Plant Biotech. J. (IF 13.8) Pub Date : 2023-10-23 Scott H. Brainard, Dean M. Sanders, Tomas Bruna, Shengqiang Shu, Julie C. Dawson
The native, perennial shrub American hazelnut (Corylus americana) is cultivated in the Midwestern United States for its significant ecological benefits, as well as its high-value nut crop. Implementation of modern breeding methods and quantitative genetic analyses of C. americana requires high-quality reference genomes, a resource that is currently lacking. We therefore developed the first chromosome-scale
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DGW1, encoding an hnRNP-like RNA binding protein, positively regulates grain size and weight by interacting with GW6 mRNA Plant Biotech. J. (IF 13.8) Pub Date : 2023-10-20 Lingfeng Li, Jijin Li, Keke Liu, Chenglong Jiang, Wenhu Jin, Jiangkun Ye, Tierui Qin, Binjiu Luo, Zeyu Chen, Jinzhao Li, Fuxiang Lv, Xiaojun Li, Haipeng Wang, Jinghua Jin, Qiming Deng, Shiquan Wang, Jun Zhu, Ting Zou, Huainian Liu, Shuangcheng Li, Ping Li, Yueyang Liang
Grain size and weight determine rice yield. Although numerous genes and pathways involved in regulating grain size have been identified, our knowledge of post-transcriptional control of grain size remains elusive. In this study, we characterize a rice mutant, decreased grain width and weight 1 (dgw1), which produces small grains. We show that DGW1 encodes a member of the heterogeneous nuclear ribonucleoprotein
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Optimizing ErCas12a for efficient gene editing in Arabidopsis thaliana Plant Biotech. J. (IF 13.8) Pub Date : 2023-10-20 Janine Pietralla, Niklas Capdeville, Patrick Schindele, Holger Puchta
The ErCas12a nuclease, also known as MAD7, is part of a CRISPR/Cas system from Eubacterium rectale and distantly related to Cas12a nucleases. As it shares only 31% sequence homology with the commonly used AsCas12a, its intellectual property may not be covered by the granted patent rights for Cas12a nucleases. Thus, ErCas12a became an attractive alternative for practical applications. However, the editing
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Coordinated gene upregulation in maize through CRISPR/Cas-mediated enhancer insertion Plant Biotech. J. (IF 13.8) Pub Date : 2023-10-20 Hannes Claeys, Eveline Neyrinck, Lies Dumoulin, Anne Pharazyn, Arne Verstichele, Laurens Pauwels, Michael L. Nuccio, Frédéric Van Ex
Genome editing technologies have significantly improved our ability to precisely modify genomes and genes, opening new possibilities to engineer endogenous pathways and traits. In crops like maize, achieving small indels, base changes and structural variation has been demonstrated (Nuccio et al., 2021). However, while these edits typically lead to gene knock-out (KO) or knock-down, improvement of many
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Down-regulation of xylan biosynthetic GhGT47Bs in cotton impedes fibre elongation and secondary wall thickening during fibre transition Plant Biotech. J. (IF 13.8) Pub Date : 2023-10-20 Yanjun Guo, Yanan Gao, Feng Chen, Jingwen Luo, Mengfei Qiao, Mengyun Li, Staffan Persson, Wei Zeng, Wenliang Xu
Cotton provides abundant natural fibres for the textile industry. Cotton fibre development can be divided into five stages: fibre initiation, elongation, transition, secondary cell wall (SCW) thickening and maturation. Fibre initiation and elongation have been extensively studied, but the transition stage remains less investigated. Although cellulose accounts for >90% of mature fibres, non-cellulosic
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Immune-enhancing miPEPs reduce plant diseases and offer new solutions in agriculture Plant Biotech. J. (IF 13.8) Pub Date : 2023-10-20 Mélanie Ormancey, Bruno Guillotin, Camille Ribeyre, Clémence Medina, Nathanael Jariais, Hélène San Clemente, Patrice Thuleau, Serge Plaza, Martina Beck, Jean-Philippe Combier
The biggest challenge for global agriculture today is to combat pathogens, especially fungi, with fewer and fewer active molecules available. In fact, fungi are responsible for 10%–20% of crop losses, equivalent to a loss of up to $200 billion per year (www.ars.usda.gov). Moreover, the use of current fungicides (mainly chemicals) is often associated with health and environmental issues (Fungicide_Uses_and_Risks)
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Appropriate reduction of importin-α gene expression enhances yellow dwarf disease resistance in common wheat Plant Biotech. J. (IF 13.8) Pub Date : 2023-10-19 Lina Wang, Kunpu Zhang, Zhaohui Wang, Jin Yang, Guozhang Kang, Yan Liu, Liyuan You, Xifeng Wang, Huaibing Jin, Daowen Wang, Tiancai Guo
Barley yellow dwarf viruses (BYDVs) cause widespread damage to global cereal crops. Here we report a novel strategy for elevating resistance to BYDV infection. The 17K protein, a potent virulence factor conserved in BYDVs, interacted with barley IMP-α1 and -α2 proteins that are nuclear transport receptors. Consistently, a nuclear localization signal was predicted in 17K, which was found essential for
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Targeted mutagenesis of flavonoid biosynthesis pathway genes reveals functional divergence in seed coat colour, oil content and fatty acid composition in Brassica napus L. Plant Biotech. J. (IF 13.8) Pub Date : 2023-10-19 Huailin Li, Kaidi Yu, Zilu Zhang, Yalun Yu, Jiakai Wan, Hanzi He, Chuchuan Fan
Yellow-seed is widely accepted as a good-quality trait in Brassica crops. Previous studies have shown that the flavonoid biosynthesis pathway is essential for the development of seed colour, but its function in Brassica napus, an important oil crop, is poorly understood. To systematically explore the gene functions of the flavonoid biosynthesis pathway in rapeseed, several representative TRANSPARENT
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RAS signalling genes can be used as host-induced gene silencing targets to control fungal diseases caused by Sclerotinia sclerotiorum and Botrytis cinerea Plant Biotech. J. (IF 13.8) Pub Date : 2023-10-16 Yan Xu, Jinyi Tan, Junxing Lu, Yuelin Zhang, Xin Li
Sclerotinia sclerotiorum causes white mold (also called stem rot, Sclerotinia blight, etc.) in many economically important plants. It is a notorious soilborne fungal pathogen due to its wide host range and ability to survive in soil for long periods of time as sclerotia. Although host-induced gene silencing (HIGS) was recently demonstrated to be an effective method for controlling white mold, limited
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CRISPR/Cas9-mediated mutation of Eil1 transcription factor genes affects exogenous ethylene tolerance and early flower senescence in Campanula portenschlagiana Plant Biotech. J. (IF 13.8) Pub Date : 2023-10-12 Inger B. Holme, Christina R. Ingvardsen, Giuseppe Dionisio, Dagmara Podzimska-Sroka, Kell Kristiansen, Anders Feilberg, Henrik Brinch-Pedersen
Improving tolerance to ethylene-induced early senescence of flowers and fruits is of major economic importance for the ornamental and food industry. Genetic modifications of genes in the ethylene-signalling pathway have frequently resulted in increased tolerance but often with unwanted side effects. Here, we used CRISPR/Cas9 to knockout the function of two CpEil1 genes expressed in flowers of the diploid
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Specific members of the TOPLESS family are susceptibility genes for Fusarium wilt in tomato and Arabidopsis Plant Biotech. J. (IF 13.8) Pub Date : 2023-10-11 Thomas R. Aalders, Mara de Sain, Fleur Gawehns, Nina Oudejans, Yoran D. Jak, Henk L. Dekker, Martijn Rep, Harrold A. van den Burg, Frank L.W. Takken
Vascular wilt diseases caused by Fusarium oxysporum are a major threat to many agriculturally important crops. Genetic resistance is rare and inevitably overcome by the emergence of new races. To identify potentially durable and non-race-specific genetic resistance against Fusarium wilt diseases, we set out to identify effector targets in tomato that mediate susceptibility to the fungus. For this purpose
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Genome editing in rice using CRISPR/Cas12i3 Plant Biotech. J. (IF 13.8) Pub Date : 2023-10-11 Ping Lv, Fei Su, Fangyuan Chen, Chunxue Yan, Dandan Xia, Hui Sun, Shanshan Li, Zhiqiang Duan, Changle Ma, Hui Zhang, Mugui Wang, Xiaomu Niu, Jian-Kang Zhu, Jinshan Zhang
The CRISPR/Cas type V-I is a family of programmable nuclease systems that prefers a T-rich protospacer adjacent motif (PAM) and is guided by a short crRNA. In this study, the genome-editing application of Cas12i3, a type V-I family endonuclease, was characterized in rice. We developed a CRIPSR/Cas12i3-based Multiplex direct repeats (DR)-spacer Array Genome Editing (iMAGE) system that was efficient
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Guide RNA scaffold variants enabled easy cloning of large gRNA cluster for multiplexed gene editing Plant Biotech. J. (IF 13.8) Pub Date : 2023-10-10 Yaqi Wang, Xiaofei Li, Minglei Liu, Yingjia Zhou, Feng Li
Cas9 protein-mediated gene editing has revolutionized genetic manipulation in most organisms. There are many cases where multiplexed gene editing is needed. Cas9 is capable of multiplex gene editing when expressed with multiple guide RNAs. Conventional cloning methods for multiplexed gene editing vector is not efficient due to repeated use of a single-guide RNA scaffold and inefficient ligation. In
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Banana MaNAC1 activates secondary cell wall cellulose biosynthesis to enhance chilling resistance in fruit Plant Biotech. J. (IF 13.8) Pub Date : 2023-10-10 Qi Yin, Wenqi Qin, Zibin Zhou, Ai-Min Wu, Wei Deng, Zhengguo Li, Wei Shan, Jian-ye Chen, Jian-fei Kuang, Wang-jin Lu
Chilling injury has a negative impact on the quantity and quality of crops, especially subtropical and tropical plants. The plant cell wall is not only the main source of biomass production, but also the first barrier to various stresses. Therefore, improving the understanding of the alterations in cell wall architecture is of great significance for both biomass production and stress adaptation. Herein
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Simultaneous suppression of lignin, tricin and wall-bound phenolic biosynthesis via the expression of monolignol 4-O-methyltransferases in rice Plant Biotech. J. (IF 13.8) Pub Date : 2023-10-05 Nidhi Dwivedi, Senri Yamamoto, Yunjun Zhao, Guichuan Hou, Forrest Bowling, Yuki Tobimatsu, Chang-Jun Liu
Grass lignocelluloses feature complex compositions and structures. In addition to the presence of conventional lignin units from monolignols, acylated monolignols and flavonoid tricin also incorporate into lignin polymer; moreover, hydroxycinnamates, particularly ferulate, cross-link arabinoxylan chains with each other and/or with lignin polymers. These structural complexities make grass lignocellulosics
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The miR156/SPL12 module orchestrates fruit colour change through directly regulating ethylene production pathway in blueberry Plant Biotech. J. (IF 13.8) Pub Date : 2023-10-05 Hongxue Li, Shouwen Wang, Lulu Zhai, Yuhai Cui, Guiliang Tang, Junwei Huo, Xuyan Li, Shaomin Bian
Colour change is an important event during fruit ripening in blueberry. It is well known that miR156/SPLs act as regulatory modules mediating anthocyanin biosynthesis and ethylene plays critical roles during colour change, but the intrinsic connections between the two pathways remain poorly understood. Previously, we demonstrated that blueberry VcMIR156a/VcSPL12 affects the accumulation of anthocyanins
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The exocyst subunit CsExo70B promotes both fruit length and disease resistance via regulating receptor kinase abundance at plasma membrane in cucumber Plant Biotech. J. (IF 13.8) Pub Date : 2023-10-05 Liu Liu, Jiacai Chen, Chaoheng Gu, Shaoyun Wang, Yufan Xue, Zhongyi Wang, Lijie Han, Weiyuan Song, Xiaofeng Liu, Jiahao Zhang, Min Li, Chuang Li, Liming Wang, Xiaolan Zhang, Zhaoyang Zhou
Plant defence against pathogens generally occurs at the expense of growth and yield. Uncoupling the inverse relationship between growth and defence is of great importance for crop breeding, while the underlying genes and regulatory mechanisms remain largely elusive. The exocytosis complex was shown to play an important role in the trafficking of receptor kinases (RKs) to the plasma membrane (PM). Here
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Prime editing: Mechanism insight and recent applications in plants Plant Biotech. J. (IF 13.8) Pub Date : 2023-10-04 Tien V. Vu, Ngan Thi Nguyen, Jihae Kim, Jong Chan Hong, Jae-Yean Kim
Prime editing (PE) technology utilizes an extended prime editing guide RNA (pegRNA) to direct a fusion peptide consisting of nCas9 (H840) and reverse transcriptase (RT) to a specific location in the genome. This enables the installation of base changes at the targeted site using the extended portion of the pegRNA through RT activity. The resulting product of the RT reaction forms a 3′ flap, which can
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The GRAS protein OsDLA involves in brassinosteroid signalling and positively regulates blast resistance by forming a module with GSK2 and OsWRKY53 in rice Plant Biotech. J. (IF 13.8) Pub Date : 2023-10-05 Fanwei Meng, Xunmei Zheng, Jia Wang, Tiancheng Qiu, Qingya Yang, Kexing Fang, Vijai Bhadauria, You-Liang Peng, Wensheng Zhao
Brassinosteroids (BRs) play a crucial role in shaping the architecture of rice (Oryza sativa) plants. However, the regulatory mechanism of BR signalling in rice immunity remains largely unexplored. Here we identify a rice mutant dla, which exhibits decreased leaf angles and is insensitive to 24-epiBL (a highly active synthetic BR), resembling the BR-deficient phenotype. The dla mutation caused by a
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CRISPR/Cas9-based genome editing of 14 lipid metabolic genes reveals a sporopollenin metabolon ZmPKSB-ZmTKPR1-1/-2 required for pollen exine formation in maize Plant Biotech. J. (IF 13.8) Pub Date : 2023-10-04 Xueli An, Shaowei Zhang, Yilin Jiang, Xinze Liu, Chaowei Fang, Jing Wang, Lina Zhao, Quancan Hou, Juan Zhang, Xiangyuan Wan
Lipid biosynthesis and transport are essential for plant male reproduction. Compared with Arabidopsis and rice, relatively fewer maize lipid metabolic genic male-sterility (GMS) genes have been identified, and the sporopollenin metabolon in maize anther remains unknown. Here, we identified two maize GMS genes, ZmTKPR1-1 and ZmTKPR1-2, by CRISPR/Cas9 mutagenesis of 14 lipid metabolic genes with anther
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ZmNRT1.1B (ZmNPF6.6) determines nitrogen use efficiency via regulation of nitrate transport and signalling in maize Plant Biotech. J. (IF 13.8) Pub Date : 2023-10-02 Huairong Cao, Zhi Liu, Jia Guo, Zhongtao Jia, Yandong Shi, Kai Kang, Wushuang Peng, Zhangkui Wang, Limei Chen, Benjamin Neuhaeuser, Yong Wang, Xiangguo Liu, Dongyun Hao, Lixing Yuan
Nitrate (NO3−) is crucial for optimal plant growth and development and often limits crop productivity under low availability. In comparison with model plant Arabidopsis, the molecular mechanisms underlying NO3− acquisition and utilization remain largely unclear in maize. In particular, only a few genes have been exploited to improve nitrogen use efficiency (NUE). Here, we demonstrated that NO3−-inducible