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  • Nesprin-2G tension fine-tunes Wnt/β-catenin signaling.
    J. Cell Biol. (IF 8.811) Pub Date : 2020-10-05
    Cara J Gottardi,G W Gant Luxton

    How LINC complexes mediate nuclear mechanotransduction remains unclear. In this issue, Déjardin, Carollo, et al. (2020. J. Cell Biol.https://doi.org/10.1083/jcb.201908036) show that the LINC complex protein nesprin-2G is a mechanosensor of epithelial-mesenchymal transitions (EMTs), recruiting α-catenin to the nucleus to attenuate Wnt/β-catenin signaling.

    更新日期:2020-09-25
  • Hands and feet: Closer than you think in epithelial migration.
    J. Cell Biol. (IF 8.811) Pub Date : 2020-10-05
    Shafali Gupta,Alpha S Yap

    Epithelial migration requires that substrate-based motility be coordinated with cell-cell adhesion. In this issue, Ozawa et al. (2020. J. Cell Biol.https://doi.org/10.1083/jcb.202006196) identify a central role for actin assembly at adherens junctions that contributes to both of these processes.

    更新日期:2020-09-25
  • An antibody toolbox to track complex I assembly defines AIF's mitochondrial function.
    J. Cell Biol. (IF 8.811) Pub Date : 2020-10-05
    Anjaneyulu Murari,Shauna-Kay Rhooms,Naga Sri Goparaju,Maximino Villanueva,Edward Owusu-Ansah

    An ability to comprehensively track the assembly intermediates (AIs) of complex I (CI) biogenesis in Drosophila will enable the characterization of the precise mechanism(s) by which various CI regulators modulate CI assembly. Accordingly, we generated 21 novel antibodies to various mitochondrial proteins and used this resource to characterize the mechanism by which apoptosis-inducing factor (AIF) regulates

    更新日期:2020-09-25
  • Stable inheritance of CENP-A chromatin: Inner strength versus dynamic control.
    J. Cell Biol. (IF 8.811) Pub Date : 2020-10-05
    Sreyoshi Mitra,Bharath Srinivasan,Lars E T Jansen

    Chromosome segregation during cell division is driven by mitotic spindle attachment to the centromere region on each chromosome. Centromeres form a protein scaffold defined by chromatin featuring CENP-A, a conserved histone H3 variant, in a manner largely independent of local DNA cis elements. CENP-A nucleosomes fulfill two essential criteria to epigenetically identify the centromere. They undergo

    更新日期:2020-09-25
  • Sensing of nutrients by CPT1C controls SAC1 activity to regulate AMPA receptor trafficking.
    J. Cell Biol. (IF 8.811) Pub Date : 2020-10-05
    Maria Casas,Rut Fadó,José Luis Domínguez,Aina Roig,Moena Kaku,Shigeru Chohnan,Montse Solé,Mercedes Unzeta,Alfredo Jesús Miñano-Molina,José Rodríguez-Álvarez,Eamonn James Dickson,Núria Casals

    Carnitine palmitoyltransferase 1C (CPT1C) is a sensor of malonyl-CoA and is located in the ER of neurons. AMPA receptors (AMPARs) mediate fast excitatory neurotransmission in the brain and play a key role in synaptic plasticity. In the present study, we demonstrate across different metabolic stress conditions that modulate malonyl-CoA levels in cortical neurons that CPT1C regulates the trafficking

    更新日期:2020-09-25
  • FIT2 is an acyl-coenzyme A diphosphatase crucial for endoplasmic reticulum homeostasis.
    J. Cell Biol. (IF 8.811) Pub Date : 2020-10-05
    Michel Becuwe,Laura M Bond,Antonio F M Pinto,Sebastian Boland,Niklas Mejhert,Shane D Elliott,Marcelo Cicconet,Morven M Graham,Xinran N Liu,Olga Ilkayeva,Alan Saghatelian,Tobias C Walther,Robert V Farese

    The endoplasmic reticulum is a cellular hub of lipid metabolism, coordinating lipid synthesis with continuous changes in metabolic flux. Maintaining ER lipid homeostasis despite these fluctuations is crucial to cell function and viability. Here, we identify a novel mechanism that is crucial for normal ER lipid metabolism and protects the ER from dysfunction. We identify the molecular function of the

    更新日期:2020-09-25
  • NUCKS1 promotes RAD54 activity in homologous recombination DNA repair.
    J. Cell Biol. (IF 8.811) Pub Date : 2020-10-05
    David G Maranon,Neelam Sharma,Yuxin Huang,Platon Selemenakis,Meiling Wang,Noelia Altina,Weixing Zhao,Claudia Wiese

    NUCKS1 (nuclear ubiquitous casein kinase and cyclin-dependent kinase substrate 1) is a chromatin-associated, vertebrate-specific, and multifunctional protein with a role in DNA damage signaling and repair. Previously, we have shown that NUCKS1 helps maintain homologous recombination (HR) DNA repair in human cells and functions as a tumor suppressor in mice. However, the mechanisms by which NUCKS1 positively

    更新日期:2020-09-25
  • Conserved actin machinery drives microtubule-independent motility and phagocytosis in Naegleria.
    J. Cell Biol. (IF 8.811) Pub Date : 2020-09-23
    Katrina B Velle,Lillian K Fritz-Laylin

    Much of our understanding of actin-driven phenotypes in eukaryotes has come from the "yeast-to-human" opisthokont lineage and the related amoebozoa. Outside of these groups lies the genus Naegleria, which shared a common ancestor with humans >1 billion years ago and includes the "brain-eating amoeba." Unlike nearly all other known eukaryotic cells, Naegleria amoebae lack interphase microtubules; this

    更新日期:2020-09-23
  • STRIPAK regulates Slik localization to control mitotic morphogenesis and epithelial integrity.
    J. Cell Biol. (IF 8.811) Pub Date : 2020-09-23
    Camille Valérie De Jamblinne,Barbara Decelle,Mehrnoush Dehghani,Mathieu Joseph,Neera Sriskandarajah,Kévin Leguay,Basile Rambaud,Sébastien Lemieux,Philippe P Roux,David R Hipfner,Sébastien Carréno

    Proteins of the ezrin, radixin, and moesin (ERM) family control cell and tissue morphogenesis. We previously reported that moesin, the only ERM in Drosophila, controls mitotic morphogenesis and epithelial integrity. We also found that the Pp1-87B phosphatase dephosphorylates moesin, counteracting its activation by the Ste20-like kinase Slik. To understand how this signaling pathway is itself regulated

    更新日期:2020-09-23
  • Tissue-wide coordination of epithelium-to-neural stem cell transition in the Drosophila optic lobe requires Neuralized.
    J. Cell Biol. (IF 8.811) Pub Date : 2020-09-19
    Chloé Shard,Juan Luna-Escalante,François Schweisguth

    Many tissues are produced by specialized progenitor cells emanating from epithelia via epithelial-to-mesenchymal transition (EMT). Most studies have so far focused on EMT involving single or isolated groups of cells. Here we describe an EMT-like process that requires tissue-level coordination. This EMT-like process occurs along a continuous front in the Drosophila optic lobe neuroepithelium to produce

    更新日期:2020-09-19
  • Mitophagy pathways in health and disease.
    J. Cell Biol. (IF 8.811) Pub Date : 2020-09-15
    Samuel A Killackey,Dana J Philpott,Stephen E Girardin

    Mitophagy is an evolutionarily conserved process involving the autophagic targeting and clearance of mitochondria destined for removal. Recent insights into the complex nature of the overlapping pathways regulating mitophagy illustrate mitophagy's essential role in maintaining the health of the mitochondrial network. In this review, we highlight recent studies that have changed the way mitophagy is

    更新日期:2020-09-15
  • A conserved myotubularin-related phosphatase regulates autophagy by maintaining autophagic flux.
    J. Cell Biol. (IF 8.811) Pub Date : 2020-09-12
    Elizabeth A Allen,Clelia Amato,Tina M Fortier,Panagiotis Velentzas,Will Wood,Eric H Baehrecke

    Macroautophagy (autophagy) targets cytoplasmic cargoes to the lysosome for degradation. Like all vesicle trafficking, autophagy relies on phosphoinositide identity, concentration, and localization to execute multiple steps in this catabolic process. Here, we screen for phosphoinositide phosphatases that influence autophagy in Drosophila and identify CG3530. CG3530 is homologous to the human MTMR6 subfamily

    更新日期:2020-09-12
  • Adherens junction regulates cryptic lamellipodia formation for epithelial cell migration.
    J. Cell Biol. (IF 8.811) Pub Date : 2020-10-05
    Masayuki Ozawa,Sylvain Hiver,Takaki Yamamoto,Tatsuo Shibata,Srigokul Upadhyayula,Yuko Mimori-Kiyosue,Masatoshi Takeichi

    Collective migration of epithelial cells plays crucial roles in various biological processes such as cancer invasion. In migrating epithelial sheets, leader cells form lamellipodia to advance, and follower cells also form similar motile apparatus at cell-cell boundaries, which are called cryptic lamellipodia (c-lamellipodia). Using adenocarcinoma-derived epithelial cells, we investigated how c-lamellipodia

    更新日期:2020-09-11
  • Genetically encoded live-cell sensor for tyrosinated microtubules.
    J. Cell Biol. (IF 8.811) Pub Date : 2020-10-05
    Shubham Kesarwani,Prakash Lama,Anchal Chandra,P Purushotam Reddy,A S Jijumon,Satish Bodakuntla,Balaji M Rao,Carsten Janke,Ranabir Das,Minhajuddin Sirajuddin

    Microtubule cytoskeleton exists in various biochemical forms in different cells due to tubulin posttranslational modifications (PTMs). Tubulin PTMs are known to affect microtubule stability, dynamics, and interaction with MAPs and motors in a specific manner, widely known as tubulin code hypothesis. At present, there exists no tool that can specifically mark tubulin PTMs in living cells, thus severely

    更新日期:2020-09-11
  • Object detection networks and augmented reality for cellular detection in fluorescence microscopy.
    J. Cell Biol. (IF 8.811) Pub Date : 2020-10-05
    Dominic Waithe,Jill M Brown,Katharina Reglinski,Isabel Diez-Sevilla,David Roberts,Christian Eggeling

    Object detection networks are high-performance algorithms famously applied to the task of identifying and localizing objects in photography images. We demonstrate their application for the classification and localization of cells in fluorescence microscopy by benchmarking four leading object detection algorithms across multiple challenging 2D microscopy datasets. Furthermore we develop and demonstrate

    更新日期:2020-09-11
  • The LTB4-BLT1 axis regulates actomyosin and β2-integrin dynamics during neutrophil extravasation.
    J. Cell Biol. (IF 8.811) Pub Date : 2020-10-05
    Bhagawat C Subramanian,Nicolas Melis,Desu Chen,Weiye Wang,Devorah Gallardo,Roberto Weigert,Carole A Parent

    The eicosanoid leukotriene B4 (LTB4) relays chemotactic signals to direct neutrophil migration to inflamed sites through its receptor BLT1. However, the mechanisms by which the LTB4-BLT1 axis relays chemotactic signals during intravascular neutrophil response to inflammation remain unclear. Here, we report that LTB4 produced by neutrophils acts as an autocrine/paracrine signal to direct the vascular

    更新日期:2020-09-11
  • Mitotic phosphorylation of Pex14p regulates peroxisomal import machinery.
    J. Cell Biol. (IF 8.811) Pub Date : 2020-10-05
    Koichiro Yamashita,Shigehiko Tamura,Masanori Honsho,Hiroto Yada,Yuichi Yagita,Hidetaka Kosako,Yukio Fujiki

    Peroxisomal matrix proteins are imported into peroxisomes via membrane-bound docking/translocation machinery. One central component of this machinery is Pex14p, a peroxisomal membrane protein involved in the docking of Pex5p, the receptor for peroxisome targeting signal type 1 (PTS1). Studies in several yeast species have shown that Pex14p is phosphorylated in vivo, whereas no function has been assigned

    更新日期:2020-09-11
  • Peroxisome retention involves Inp1-dependent peroxisome-plasma membrane contact sites in yeast.
    J. Cell Biol. (IF 8.811) Pub Date : 2020-10-05
    Arjen M Krikken,Huala Wu,Rinse de Boer,Damien P Devos,Tim P Levine,Ida J van der Klei

    Retention of peroxisomes in yeast mother cells requires Inp1, which is recruited to the organelle by the peroxisomal membrane protein Pex3. Here we show that Hansenula polymorpha Inp1 associates peroxisomes to the plasma membrane. Peroxisome-plasma membrane contact sites disappear upon deletion of INP1 but increase upon INP1 overexpression. Analysis of truncated Inp1 variants showed that the C terminus

    更新日期:2020-09-11
  • Wnts regulate planar cell polarity via heterotrimeric G protein and PI3K signaling.
    J. Cell Biol. (IF 8.811) Pub Date : 2020-10-05
    Andre Landin Malt,Arielle K Hogan,Connor D Smith,Maxwell S Madani,Xiaowei Lu

    In the mammalian cochlea, the planar cell polarity (PCP) pathway aligns hair cell orientation along the plane of the sensory epithelium. Concurrently, multiple cell intrinsic planar polarity (referred to as iPCP) modules mediate planar polarization of the hair cell apical cytoskeleton, including the kinocilium and the V-shaped hair bundle essential for mechanotransduction. How PCP and iPCP are coordinated

    更新日期:2020-09-11
  • Keeping track of time: The fundamentals of cellular clocks.
    J. Cell Biol. (IF 8.811) Pub Date : 2020-09-10
    Colin R Gliech,Andrew J Holland

    Biological timekeeping enables the coordination and execution of complex cellular processes such as developmental programs, day/night organismal changes, intercellular signaling, and proliferative safeguards. While these systems are often considered separately owing to a wide variety of mechanisms, time frames, and outputs, all clocks are built by calibrating or delaying the rate of biochemical reactions

    更新日期:2020-09-10
  • Correction: Role of the early secretory pathway in SARS-CoV-2 infection.
    J. Cell Biol. (IF 8.811) Pub Date : 2020-09-07
    Daria Sicari,Aristotelis Chatziioannou,Theodoros Koutsandreas,Roberto Sitia,Eric Chevet

    更新日期:2020-09-07
  • Programming pluripotent stem cells: Can't teach an old cell new DNA replication tricks.
    J. Cell Biol. (IF 8.811) Pub Date : 2020-09-07
    Brandon L Mouery,Liu Mei,Jeanette Gowen Cook

    Pluripotent stem cells differentiate with varying efficiencies depending on the method of reprogramming that created them. In this issue, Paniza et al. (2020. J. Cell Biol.https://doi.org/10.1083/jcb.201909163) demonstrate that cells with lower differentiation potential retain some features of somatic DNA replication origin utilization and suffer more frequent DNA damage.

    更新日期:2020-09-07
  • Long noncoding RNA amplified in lung cancer rewires cancer pathways.
    J. Cell Biol. (IF 8.811) Pub Date : 2020-09-07
    Elena Martínez-Terroba,Nadya Dimitrova

    Athie et al. (2020. J. Cell Biol. https://doi.org/10.1083/jcb.201908078) identify ALAL-1, a lncRNA frequently amplified or overexpressed in lung cancer, as an oncogenic driver, capable of promoting the proliferation and altering the immunogenicity of lung cancer cells.

    更新日期:2020-09-07
  • Analysis of copy number alterations reveals the lncRNA ALAL-1 as a regulator of lung cancer immune evasion.
    J. Cell Biol. (IF 8.811) Pub Date : 2020-09-07
    Alejandro Athie,Francesco P Marchese,Jovanna González,Teresa Lozano,Ivan Raimondi,Prasanna Kumar Juvvuna,Amaya Abad,Oskar Marin-Bejar,Jacques Serizay,Dannys Martínez,Daniel Ajona,Maria Jose Pajares,Juan Sandoval,Luis M Montuenga,Chandrasekhar Kanduri,Juan J Lasarte,Maite Huarte

    Cancer is characterized by genomic instability leading to deletion or amplification of oncogenes or tumor suppressors. However, most of the altered regions are devoid of known cancer drivers. Here, we identify lncRNAs frequently lost or amplified in cancer. Among them, we found amplified lncRNA associated with lung cancer-1 (ALAL-1) as frequently amplified in lung adenocarcinomas. ALAL-1 is also overexpressed

    更新日期:2020-09-07
  • Mitophagy is induced by short ubiquitin chains on mitochondria.
    J. Cell Biol. (IF 8.811) Pub Date : 2020-09-07
    Fumiyo Ikeda

    Mitophagy has a critical role in maintaining cellular homeostasis by removing damaged mitochondria. In this issue, Yamano et al. (2020. J. Cell Biol. https://doi.org/10.1083/jcb.201912144) uncover that a novel complex of the autophagy adaptor optineurin and the membrane protein ATG9A specifically regulate ubiquitin-induced mitophagy.

    更新日期:2020-09-07
  • p53 deficiency triggers dysregulation of diverse cellular processes in physiological oxygen.
    J. Cell Biol. (IF 8.811) Pub Date : 2020-09-05
    Liz J Valente,Amy Tarangelo,Albert Mao Li,Marwan Naciri,Nitin Raj,Anthony M Boutelle,Yang Li,Stephano Spano Mello,Kathryn Bieging-Rolett,Ralph J DeBerardinis,Jiangbin Ye,Scott J Dixon,Laura D Attardi

    The mechanisms by which TP53, the most frequently mutated gene in human cancer, suppresses tumorigenesis remain unclear. p53 modulates various cellular processes, such as apoptosis and proliferation, which has led to distinct cellular mechanisms being proposed for p53-mediated tumor suppression in different contexts. Here, we asked whether during tumor suppression p53 might instead regulate a wide

    更新日期:2020-09-05
  • Loss of CBX2 induces genome instability and senescence-associated chromosomal rearrangements.
    J. Cell Biol. (IF 8.811) Pub Date : 2020-09-02
    Claudia Baumann,Xiangyu Zhang,Rabindranath De La Fuente

    The polycomb group protein CBX2 is an important epigenetic reader involved in cell proliferation and differentiation. While CBX2 overexpression occurs in a wide range of human tumors, targeted deletion results in homeotic transformation, proliferative defects, and premature senescence. However, its cellular function(s) and whether it plays a role in maintenance of genome stability remain to be determined

    更新日期:2020-09-02
  • Terminal web and vesicle trafficking proteins mediate nematode single-cell tubulogenesis.
    J. Cell Biol. (IF 8.811) Pub Date : 2020-08-30
    Zhe Yang,Brendan C Mattingly,David H Hall,Brian D Ackley,Matthew Buechner

    Single-celled tubules represent a complicated structure that forms during development, requiring extension of a narrow cytoplasm surrounding a lumen exerting osmotic pressure that can burst the luminal membrane. Genetic studies on the excretory canal cell of Caenorhabditis elegans have revealed many proteins that regulate the cytoskeleton, vesicular transport, and physiology of the narrow canals. Here

    更新日期:2020-08-30
  • The septin cytoskeleton regulates natural killer cell lytic granule release.
    J. Cell Biol. (IF 8.811) Pub Date : 2020-08-26
    Prasad V Phatarpekar,Brittany L Overlee,Alexander Leehan,Katelynn M Wilton,Hyoungjun Ham,Daniel D Billadeau

    Natural killer (NK) cell-mediated killing involves the membrane fusion of preformed lytic granules. While the roles of actin and microtubules are well accepted during this process, the function of septins, another cytoskeletal component that associates with actin and microtubules, has not been investigated. Here we show that genetic depletion or pharmacologic stabilization of the septin cytoskeleton

    更新日期:2020-08-26
  • CDC42EP5/BORG3 modulates SEPT9 to promote actomyosin function, migration, and invasion.
    J. Cell Biol. (IF 8.811) Pub Date : 2020-09-07
    Aaron J Farrugia,Javier Rodríguez,Jose L Orgaz,María Lucas,Victoria Sanz-Moreno,Fernando Calvo

    Fast amoeboid migration is critical for developmental processes and can be hijacked by cancer cells to enhance metastatic dissemination. This migratory behavior is tightly controlled by high levels of actomyosin contractility, but how it is coupled to other cytoskeletal components is poorly understood. Septins are increasingly recognized as novel cytoskeletal components, but details on their regulation

    更新日期:2020-08-18
  • Evolving models for assembling and shaping clathrin-coated pits.
    J. Cell Biol. (IF 8.811) Pub Date : 2020-09-07
    Zhiming Chen,Sandra L Schmid

    Clathrin-mediated endocytosis occurs via the assembly of clathrin-coated pits (CCPs) that invaginate and pinch off to form clathrin-coated vesicles (CCVs). It is well known that adaptor protein 2 (AP2) complexes trigger clathrin assembly on the plasma membrane, and biochemical and structural studies have revealed the nature of these interactions. Numerous endocytic accessory proteins collaborate with

    更新日期:2020-08-14
  • Role of the early secretory pathway in SARS-CoV-2 infection.
    J. Cell Biol. (IF 8.811) Pub Date : 2020-09-07
    Daria Sicari,Aristotelis Chatziioannou,Theodoros Koutsandreas,Roberto Sitia,Eric Chevet

    Similar to other RNA viruses, SARS-CoV-2 must (1) enter a target/host cell, (2) reprogram it to ensure its replication, (3) exit the host cell, and (4) repeat this cycle for exponential growth. During the exit step, the virus hijacks the sophisticated machineries that host cells employ to correctly fold, assemble, and transport proteins along the exocytic pathway. Therefore, secretory pathway-mediated

    更新日期:2020-08-14
  • G1/S transcription factors assemble in increasing numbers of discrete clusters through G1 phase.
    J. Cell Biol. (IF 8.811) Pub Date : 2020-09-07
    Labe Black,Sylvain Tollis,Guo Fu,Jean-Bernard Fiche,Savanna Dorsey,Jing Cheng,Ghada Ghazal,Stephen Notley,Benjamin Crevier,Jeremy Bigness,Marcelo Nollmann,Mike Tyers,Catherine Ann Royer

    In budding yeast, the transcription factors SBF and MBF activate a large program of gene expression in late G1 phase that underlies commitment to cell division, termed Start. SBF/MBF are limiting with respect to target promoters in small G1 phase cells and accumulate as cells grow, raising the questions of how SBF/MBF are dynamically distributed across the G1/S regulon and how this impacts the Start

    更新日期:2020-08-14
  • Deorphanizing FAM19A proteins as pan-neurexin ligands with an unusual biosynthetic binding mechanism.
    J. Cell Biol. (IF 8.811) Pub Date : 2020-09-07
    Anna J Khalaj,Fredrik H Sterky,Alessandra Sclip,Jochen Schwenk,Axel T Brunger,Bernd Fakler,Thomas C Südhof

    Neurexins are presynaptic adhesion molecules that organize synapses by binding to diverse trans-synaptic ligands, but how neurexins are regulated is incompletely understood. Here we identify FAM19A/TAFA proteins, "orphan" cytokines, as neurexin regulators that interact with all neurexins, except for neurexin-1γ, via an unusual mechanism. Specifically, we show that FAM19A1-A4 bind to the cysteine-loop

    更新日期:2020-08-14
  • mRNA localization mediates maturation of cytoplasmic cilia in Drosophila spermatogenesis.
    J. Cell Biol. (IF 8.811) Pub Date : 2020-09-07
    Jaclyn M Fingerhut,Yukiko M Yamashita

    Cytoplasmic cilia, a specialized type of cilia in which the axoneme resides within the cytoplasm rather than within the ciliary compartment, are proposed to allow for the efficient assembly of very long cilia. Despite being found diversely in male gametes (e.g., Plasmodium falciparum microgametocytes and human and Drosophila melanogaster sperm), very little is known about cytoplasmic cilia assembly

    更新日期:2020-08-14
  • Pluripotent stem cells with low differentiation potential contain incompletely reprogrammed DNA replication.
    J. Cell Biol. (IF 8.811) Pub Date : 2020-09-07
    Theodore Paniza,Madhura Deshpande,Ning Wang,Ryan O'Neil,Michael V Zuccaro,Morgan Elizabeth Smith,Advaitha Madireddy,Daylon James,Joseph Ecker,Zev Rosenwaks,Dieter Egli,Jeannine Gerhardt

    Reprogrammed pluripotent stem cells (PSCs) are valuable for research and potentially for cell replacement therapy. However, only a fraction of reprogrammed PSCs are developmentally competent. Genomic stability and accurate DNA synthesis are fundamental for cell development and critical for safety. We analyzed whether defects in DNA replication contribute to genomic instability and the diverse differentiation

    更新日期:2020-08-14
  • Different lineage contexts direct common pro-neural factors to specify distinct retinal cell subtypes.
    J. Cell Biol. (IF 8.811) Pub Date : 2020-09-07
    Mei Wang,Lei Du,Aih Cheun Lee,Yan Li,Huiwen Qin,Jie He

    How astounding neuronal diversity arises from variable cell lineages in vertebrates remains mostly elusive. By in vivo lineage tracing of ∼1,000 single zebrafish retinal progenitors, we identified a repertoire of subtype-specific stereotyped neurogenic lineages. Remarkably, within these stereotyped lineages, GABAergic amacrine cells were born with photoreceptor cells, whereas glycinergic amacrine cells

    更新日期:2020-08-14
  • EGF receptor-mediated FUS phosphorylation promotes its nuclear translocation and fibrotic signaling.
    J. Cell Biol. (IF 8.811) Pub Date : 2020-09-07
    Manuel Chiusa,Wen Hu,Jozef Zienkiewicz,Xiwu Chen,Ming-Zhi Zhang,Raymond C Harris,Roberto M Vanacore,Jennifer A Bentz,Giuseppe Remuzzi,Ariela Benigni,Agnes B Fogo,Wentian Luo,Stavroula Mili,Matthew H Wilson,Roy Zent,Jacek Hawiger,Ambra Pozzi

    Excessive accumulation of collagen leads to fibrosis. Integrin α1β1 (Itgα1β1) prevents kidney fibrosis by reducing collagen production through inhibition of the EGF receptor (EGFR) that phosphorylates cytoplasmic and nuclear proteins. To elucidate how the Itgα1β1/EGFR axis controls collagen synthesis, we analyzed the levels of nuclear tyrosine phosphorylated proteins in WT and Itgα1-null kidney cells

    更新日期:2020-08-14
  • Nuclear role for human Argonaute-1 as an estrogen-dependent transcription coactivator.
    J. Cell Biol. (IF 8.811) Pub Date : 2020-09-07
    Luciana I Gómez Acuña,Ezequiel Nazer,Santiago A Rodríguez-Seguí,Berta Pozzi,Valeria Buggiano,Luciano E Marasco,Eneritz Agirre,Cody He,Mariano Alló,Alberto R Kornblihtt

    In mammals, argonaute (AGO) proteins have been characterized for their roles in small RNA-mediated posttranscriptional and also in transcriptional gene silencing. Here, we report a different role for AGO1 in estradiol-triggered transcriptional activation in human cells. We show that in MCF-7 mammary gland cells, AGO1 associates with transcriptional enhancers of estrogen receptor α (ERα) and that this

    更新日期:2020-08-14
  • Intersection of TKS5 and FGD1/CDC42 signaling cascades directs the formation of invadopodia.
    J. Cell Biol. (IF 8.811) Pub Date : 2020-09-07
    Anna Zagryazhskaya-Masson,Pedro Monteiro,Anne-Sophie Macé,Alessia Castagnino,Robin Ferrari,Elvira Infante,Aléria Duperray-Susini,Florent Dingli,Arpad Lanyi,Damarys Loew,Elisabeth Génot,Philippe Chavrier

    Tumor cells exposed to a physiological matrix of type I collagen fibers form elongated collagenolytic invadopodia, which differ from dotty-like invadopodia forming on the gelatin substratum model. The related scaffold proteins, TKS5 and TKS4, are key components of the mechanism of invadopodia assembly. The molecular events through which TKS proteins direct collagenolytic invadopodia formation are poorly

    更新日期:2020-08-14
  • PTEN dephosphorylates Abi1 to promote epithelial morphogenesis.
    J. Cell Biol. (IF 8.811) Pub Date : 2020-09-07
    Yanmei Qi,Jie Liu,Joshua Chao,Peter A Greer,Shaohua Li

    The tumor suppressor PTEN is essential for early development. Its lipid phosphatase activity converts PIP3 to PIP2 and antagonizes the PI3K-Akt pathway. In this study, we demonstrate that PTEN's protein phosphatase activity is required for epiblast epithelial differentiation and polarization. This is accomplished by reconstitution of PTEN-null embryoid bodies with PTEN mutants that lack only PTEN's

    更新日期:2020-08-14
  • The kinesin-like protein Pavarotti functions noncanonically to regulate actin dynamics.
    J. Cell Biol. (IF 8.811) Pub Date : 2020-09-07
    Mitsutoshi Nakamura,Jeffrey M Verboon,Clara L Prentiss,Susan M Parkhurst

    Pavarotti, the Drosophila MKLP1 orthologue, is a kinesin-like protein that works with Tumbleweed (MgcRacGAP) as the centralspindlin complex. This complex is essential for cytokinesis, where it helps to organize the contractile actomyosin ring at the equator of dividing cells by activating the RhoGEF Pebble. Actomyosin rings also function as the driving force during cell wound repair. We previously

    更新日期:2020-08-14
  • Cancer cells educate natural killer cells to a metastasis-promoting cell state.
    J. Cell Biol. (IF 8.811) Pub Date : 2020-09-07
    Isaac S Chan,Hildur Knútsdóttir,Gayathri Ramakrishnan,Veena Padmanaban,Manisha Warrier,Juan Carlos Ramirez,Matthew Dunworth,Hao Zhang,Elizabeth M Jaffee,Joel S Bader,Andrew Josef Ewald

    Natural killer (NK) cells have potent antitumor and antimetastatic activity. It is incompletely understood how cancer cells escape NK cell surveillance. Using ex vivo and in vivo models of metastasis, we establish that keratin-14+ breast cancer cells are vulnerable to NK cells. We then discovered that exposure to cancer cells causes NK cells to lose their cytotoxic ability and promote metastatic outgrowth

    更新日期:2020-08-14
  • Alternative splicing of clathrin heavy chain contributes to the switch from coated pits to plaques.
    J. Cell Biol. (IF 8.811) Pub Date : 2020-09-07
    Gilles Moulay,Jeanne Lainé,Mégane Lemaître,Masayuki Nakamori,Ichizo Nishino,Ghislaine Caillol,Kamel Mamchaoui,Laura Julien,Florent Dingli,Damarys Loew,Marc Bitoun,Christophe Leterrier,Denis Furling,Stéphane Vassilopoulos

    Clathrin function directly derives from its coat structure, and while endocytosis is mediated by clathrin-coated pits, large plaques contribute to cell adhesion. Here, we show that the alternative splicing of a single exon of the clathrin heavy chain gene (CLTC exon 31) helps determine the clathrin coat organization. Direct genetic control was demonstrated by forced CLTC exon 31 skipping in muscle

    更新日期:2020-08-14
  • Mutual antagonism between Hippo signaling and cyclin E drives intracellular pattern formation.
    J. Cell Biol. (IF 8.811) Pub Date : 2020-09-07
    Yu-Yang Jiang,Wolfgang Maier,Uzoamaka N Chukka,Michael Choromanski,Chinkyu Lee,Ewa Joachimiak,Dorota Wloga,Wayland Yeung,Natarajan Kannan,Joseph Frankel,Jacek Gaertig

    Not much is known about how organelles organize into patterns. In ciliates, the cortical pattern is propagated during "tandem duplication," a cell division that remodels the parental cell into two daughter cells. A key step is the formation of the division boundary along the cell's equator. In Tetrahymena thermophila, the cdaA alleles prevent the formation of the division boundary. We find that the

    更新日期:2020-08-14
  • Tyramide signal amplification mass spectrometry (TSA-MS) ratio identifies nuclear speckle proteins.
    J. Cell Biol. (IF 8.811) Pub Date : 2020-09-07
    Joseph Dopie,Michael J Sweredoski,Annie Moradian,Andrew S Belmont

    We present a simple ratio method to infer protein composition within cellular structures using proximity labeling approaches but compensating for the diffusion of free radicals. We used tyramide signal amplification (TSA) and label-free mass spectrometry (MS) to compare proteins in nuclear speckles versus centromeres. Our "TSA-MS ratio" approach successfully identified known nuclear speckle proteins

    更新日期:2020-08-14
  • Branched actin networks are assembled on microtubules by adenomatous polyposis coli for targeted membrane protrusion.
    J. Cell Biol. (IF 8.811) Pub Date : 2020-09-07
    Nadia Efimova,Changsong Yang,Jonathan X Chia,Ning Li,Christopher J Lengner,Kristi L Neufeld,Tatyana M Svitkina

    Cell migration is driven by pushing and pulling activities of the actin cytoskeleton, but migration directionality is largely controlled by microtubules. This function of microtubules is especially critical for neuron navigation. However, the underlying mechanisms are poorly understood. Here we show that branched actin filament networks, the main pushing machinery in cells, grow directly from microtubule

    更新日期:2020-08-14
  • Correction: The function of GORASPs in Golgi apparatus organization in vivo.
    J. Cell Biol. (IF 8.811) Pub Date : 2020-09-07
    Rianne Grond,Tineke Veenendaal,Juan M Duran,Ishier Raote,Johan H van Es,Sebastiaan Corstjens,Laura Delfgou,Benaissa El Haddouti,Vivek Malhotra,Catherine Rabouille

    更新日期:2020-08-14
  • Crippling life support for SARS-CoV-2 and other viruses through synthetic lethality.
    J. Cell Biol. (IF 8.811) Pub Date : 2020-10-05
    Fred D Mast,Arti T Navare,Almer M van der Sloot,Jasmin Coulombe-Huntington,Michael P Rout,Nitin S Baliga,Alexis Kaushansky,Brian T Chait,Alan Aderem,Charles M Rice,Andrej Sali,Mike Tyers,John D Aitchison

    With the rapid global spread of SARS-CoV-2, we have become acutely aware of the inadequacies of our ability to respond to viral epidemics. Although disrupting the viral life cycle is critical for limiting viral spread and disease, it has proven challenging to develop targeted and selective therapeutics. Synthetic lethality offers a promising but largely unexploited strategy against infectious viral

    更新日期:2020-08-14
  • Nesprins are mechanotransducers that discriminate epithelial-mesenchymal transition programs.
    J. Cell Biol. (IF 8.811) Pub Date : 2020-08-14
    Théophile Déjardin,Pietro Salvatore Carollo,François Sipieter,Patricia M Davidson,Cynthia Seiler,Damien Cuvelier,Bruno Cadot,Cecile Sykes,Edgar R Gomes,Nicolas Borghi

    LINC complexes are transmembrane protein assemblies that physically connect the nucleoskeleton and cytoskeleton through the nuclear envelope. Dysfunctions of LINC complexes are associated with pathologies such as cancer and muscular disorders. The mechanical roles of LINC complexes are poorly understood. To address this, we used genetically encoded FRET biosensors of molecular tension in a nesprin

    更新日期:2020-08-14
  • Telomerase treatment prevents lung profibrotic pathologies associated with physiological aging.
    J. Cell Biol. (IF 8.811) Pub Date : 2020-08-11
    Sergio Piñeiro-Hermida,Chiara Autilio,Paula Martínez,Fátima Bosch,Jesús Pérez-Gil,Maria A Blasco

    Short/dysfunctional telomeres are at the origin of idiopathic pulmonary fibrosis (IPF) in patients mutant for telomere maintenance genes. However, it remains unknown whether physiological aging leads to short telomeres in the lung, thus leading to IPF with aging. Here, we find that physiological aging in wild-type mice leads to telomere shortening and a reduced proliferative potential of alveolar type

    更新日期:2020-08-11
  • Collective invasion induced by an autocrine purinergic loop through connexin-43 hemichannels.
    J. Cell Biol. (IF 8.811) Pub Date : 2020-08-11
    Antoine A Khalil,Olga Ilina,Angela Vasaturo,Jan-Hendrik Venhuizen,Manon Vullings,Victor Venhuizen,Ab Bilos,Carl G Figdor,Paul N Span,Peter Friedl

    Progression of epithelial cancers predominantly proceeds by collective invasion of cell groups with coordinated cell-cell junctions and multicellular cytoskeletal activity. Collectively invading breast cancer cells express the gap junction protein connexin-43 (Cx43), yet whether Cx43 regulates collective invasion remains unclear. We here show that Cx43 mediates gap-junctional coupling between collectively

    更新日期:2020-08-11
  • Notch2 complements Notch1 to mediate inductive signaling that initiates early T cell development.
    J. Cell Biol. (IF 8.811) Pub Date : 2020-08-07
    Maile Romero-Wolf,Boyoung Shin,Wen Zhou,Maria Koizumi,Ellen V Rothenberg,Hiroyuki Hosokawa

    Notch signaling is the dominant intercellular signaling input during the earliest stages of T cell development in the thymus. Although Notch1 is known to be indispensable, we show that it does not mediate all Notch signaling in precommitment stages: Notch2 initially works in parallel to promote early murine T cell development and antagonize other fates. Notch-regulated target genes before and after

    更新日期:2020-08-07
  • Correction: ERdj8 governs the size of autophagosomes during the formation process.
    J. Cell Biol. (IF 8.811) Pub Date : 2020-08-03
    Yo-Hei Yamamoto,Ayano Kasai,Hiroko Omori,Tomoe Takino,Munechika Sugihara,Tetsuo Umemoto,Maho Hamasaki,Tomohisa Hatta,Tohru Natsume,Richard I Morimoto,Ritsuko Arai,Satoshi Waguri,Miyuki Sato,Ken Sato,Shoshana Bar-Nun,Tamotsu Yoshimori,Takeshi Noda,Kazuhiro Nagata

    更新日期:2020-08-03
  • Zena Werb (1945-2020): Cell biology in context.
    J. Cell Biol. (IF 8.811) Pub Date : 2020-08-03
    Michael Sixt,Anna Huttenlocher

    更新日期:2020-08-03
  • Necroptosis is SARMful to your health.
    J. Cell Biol. (IF 8.811) Pub Date : 2020-08-03
    Brian A Pierchala

    Necroptosis is a cell death pathway involved in inflammation and disease. In this issue, Ko et al. (2020. J. Cell Biol.https://doi.org/10.1083/jcb.201912047) link SARM1, the executioner of Wallerian degeneration of axons, to necroptosis, revealing a unique form of axonal disassembly likely involved in neurodegenerative disorders.

    更新日期:2020-08-03
  • HDAC6 regulates microtubule stability and clustering of AChRs at neuromuscular junctions.
    J. Cell Biol. (IF 8.811) Pub Date : 2020-08-03
    Alexis Osseni,Aymeric Ravel-Chapuis,Jean-Luc Thomas,Vincent Gache,Laurent Schaeffer,Bernard J Jasmin

    Microtubules (MTs) are known to be post-translationally modified at the neuromuscular junction (NMJ), hence increasing their stability. To date however, the function(s) of the dynamic MT network and its relative stability in the formation and maintenance of NMJs remain poorly described. Stabilization of the MT is dependent in part on its acetylation status, and HDAC6 is capable of reversing this post-translational

    更新日期:2020-08-03
  • Protrudin in protrudinG invadopodia: Membrane contact sites and cell invasion.
    J. Cell Biol. (IF 8.811) Pub Date : 2020-08-03
    Amita Arora,Vesa M Olkkonen

    Invadopodia are dynamic protrusions that harbor matrix metalloproteinases for pericellular matrix degradation. However, the mechanisms underlying their maturation are poorly understood. Pedersen et al. (2020. J. Cell Biol.https://doi.org/10.1083/jcb.202003063) demonstrate a dual role of Protrudin in invadopodia elongation and matrix degradation, central to cell invasion and cancer metastasis.

    更新日期:2020-08-03
  • ISG15 fast-tracks DNA replication.
    J. Cell Biol. (IF 8.811) Pub Date : 2020-08-03
    Alice Meroni,Alessandro Vindigni

    In this issue, Raso et al. (2020. J. Cell Biol.https://doi.org/10.1083/jcb.202002175) uncover a novel replication fork speed regulatory network controlled by the ubiquitin-like modifier interferon-stimulated gene 15 (ISG15), which plays a central role in the innate immune response and regulates tumorigenesis as well as chemotherapy response.

    更新日期:2020-08-03
  • Phospholipid ebb and flow makes mitochondria go.
    J. Cell Biol. (IF 8.811) Pub Date : 2020-08-03
    Michelle Grace Acoba,Nanami Senoo,Steven M Claypool

    Mitochondria, so much more than just being energy factories, also have the capacity to synthesize macromolecules including phospholipids, particularly cardiolipin (CL) and phosphatidylethanolamine (PE). Phospholipids are vital constituents of mitochondrial membranes, impacting the plethora of functions performed by this organelle. Hence, the orchestrated movement of phospholipids to and from the mitochondrion

    更新日期:2020-08-03
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