当前期刊: Nature Chemical Biology Go to current issue    加入关注   
显示样式:        排序: IF: - GO 导出
我的关注
我的收藏
您暂时未登录!
登录
  • Formation and functionalization of membraneless compartments in Escherichia coli
    Nat. Chem. Biol. (IF 12.587) Pub Date : 2020-06-29
    Shao-Peng Wei; Zhi-Gang Qian; Chun-Fei Hu; Fang Pan; Meng-Ting Chen; Sang Yup Lee; Xiao-Xia Xia

    Membraneless organelles formed by liquid–liquid phase separation of proteins or nucleic acids are involved in diverse biological processes in eukaryotes. However, such cellular compartments have yet to be discovered or created synthetically in prokaryotes. Here, we report the formation of liquid protein condensates inside the cells of prokaryotic Escherichia coli upon heterologous overexpression of

    更新日期:2020-06-29
  • Tetracenomycin X inhibits translation by binding within the ribosomal exit tunnel
    Nat. Chem. Biol. (IF 12.587) Pub Date : 2020-06-29
    Ilya A. Osterman; Maximiliane Wieland; Tinashe P. Maviza; Kseniya A. Lashkevich; Dmitrii A. Lukianov; Ekaterina S. Komarova; Yuliya V. Zakalyukina; Robert Buschauer; Dmitrii I. Shiriaev; Semen A. Leyn; Jaime E. Zlamal; Mikhail V. Biryukov; Dmitry A. Skvortsov; Vadim N. Tashlitsky; Vladimir I. Polshakov; Jingdong Cheng; Yury S. Polikanov; Alexey A. Bogdanov; Andrei L. Osterman; Sergey E. Dmitriev; Roland

    The increase in multi-drug resistant pathogenic bacteria is making our current arsenal of clinically used antibiotics obsolete, highlighting the urgent need for new lead compounds with distinct target binding sites to avoid cross-resistance. Here we report that the aromatic polyketide antibiotic tetracenomycin (TcmX) is a potent inhibitor of protein synthesis, and does not induce DNA damage as previously

    更新日期:2020-06-29
  • The bottromycin epimerase BotH defines a group of atypical α/β-hydrolase-fold enzymes
    Nat. Chem. Biol. (IF 12.587) Pub Date : 2020-06-29
    Asfandyar Sikandar; Laura Franz; Sebastian Adam; Javier Santos-Aberturas; Liliya Horbal; Andriy Luzhetskyy; Andrew W. Truman; Olga V. Kalinina; Jesko Koehnke

    d-amino acids endow peptides with diverse, desirable properties, but the post-translational and site-specific epimerization of l-amino acids into their d-counterparts is rare and chemically challenging. Bottromycins are ribosomally synthesized and post-translationally modified peptides that have overcome this challenge and feature a d-aspartate (d-Asp), which was proposed to arise spontaneously during

    更新日期:2020-06-29
  • Publisher Correction: Structural insights into β-1,3-glucan cleavage by a glycoside hydrolase family
    Nat. Chem. Biol. (IF 12.587) Pub Date : 2020-06-25
    Camila R. Santos; Pedro A. C. R. Costa; Plínio S. Vieira; Sinkler E. T. Gonzalez; Thamy L. R. Correa; Evandro A. Lima; Fernanda Mandelli; Renan A. S. Pirolla; Mariane N. Domingues; Lucelia Cabral; Marcele P. Martins; Rosa L. Cordeiro; Atílio T. Junior; Beatriz P. Souza; Érica T. Prates; Fabio C. Gozzo; Gabriela F. Persinoti; Munir S. Skaf; Mario T. Murakami

    An amendment to this paper has been published and can be accessed via a link at the top of the paper.

    更新日期:2020-06-25
  • Better editors.
    Nat. Chem. Biol. (IF 12.587) Pub Date : 2020-07-01
    Yiyun Song

    更新日期:2020-06-24
  • Taking out the trash.
    Nat. Chem. Biol. (IF 12.587) Pub Date : 2020-07-01
    Grant Miura

    更新日期:2020-06-24
  • Passing the acid test.
    Nat. Chem. Biol. (IF 12.587) Pub Date : 2020-07-01
    Caitlin Deane

    更新日期:2020-06-24
  • Groovy RNA polymerase.
    Nat. Chem. Biol. (IF 12.587) Pub Date : 2020-07-01
    Mirella Bucci

    更新日期:2020-06-24
  • Discovering Nature's super glue.
    Nat. Chem. Biol. (IF 12.587) Pub Date : 2020-06-22
    Zhi Zeng,Ting Han

    Molecular glues induce novel protein–protein interactions to modulate protein function and downstream biology. A recent study unveils manumycin polyketides with multiple electrophilic centers as covalent molecular glues between UBR7 and TP53.

    更新日期:2020-06-23
  • Manumycin polyketides act as molecular glues between UBR7 and P53.
    Nat. Chem. Biol. (IF 12.587) Pub Date : 2020-06-22
    Yosuke Isobe,Mikiko Okumura,Lynn M McGregor,Scott M Brittain,Michael D Jones,Xiaoyou Liang,Ross White,William Forrester,Jeffrey M McKenna,John A Tallarico,Markus Schirle,Thomas J Maimone,Daniel K Nomura

    Molecular glues are an intriguing therapeutic modality that harness small molecules to induce interactions between proteins that typically do not interact. However, such molecules are rare and have been discovered fortuitously, thus limiting their potential as a general strategy for therapeutic intervention. We postulated that natural products bearing one or more electrophilic sites may be an unexplored

    更新日期:2020-06-23
  • 5-LOX inhibition by natural products.
    Nat. Chem. Biol. (IF 12.587) Pub Date : 2020-06-22
    Homero Rubbo,Irene Wood

    The enzyme 5-lipoxygenase (5-LOX) initiates the biosynthesis of leukotrienes (LT), potent mediators of the inflammatory response. The first crystal structures of two complexes of inhibitor bound to 5-LOX reveal the functional consequences of the binding, including a change in the regiospecificity toward a 12/15-lipoxygenating enzyme.

    更新日期:2020-06-23
  • The uninhibited pathway is not worth studying.
    Nat. Chem. Biol. (IF 12.587) Pub Date : 2020-06-22
    Michael E Pacold

    Glucose 6-phosphate dehydrogenase (G6PD) stands at the head of the pentose phosphate pathway, which is responsible for nucleotide synthesis. The identification and thorough validation of an improved G6PD inhibitor provide a valuable new tool compound for studying metabolism.

    更新日期:2020-06-23
  • Spectroscopic coherent Raman imaging of Caenorhabditis elegans reveals lipid particle diversity.
    Nat. Chem. Biol. (IF 12.587) Pub Date : 2020-06-22
    Wei-Wen Chen,George A Lemieux,Charles H Camp,Ta-Chau Chang,Kaveh Ashrafi,Marcus T Cicerone

    Caenorhabditis elegans serves as a model for understanding adiposity and its connections to aging. Current methodologies do not distinguish between fats serving the energy needs of the parent, akin to mammalian adiposity, from those that are distributed to the progeny, making it difficult to accurately interpret the physiological implications of fat content changes induced by external perturbations

    更新日期:2020-06-23
  • Structural insight into the formation of lipoprotein-β-barrel complexes.
    Nat. Chem. Biol. (IF 12.587) Pub Date : 2020-06-22
    Raquel Rodríguez-Alonso,Juliette Létoquart,Van Son Nguyen,Gwennaelle Louis,Antonio N Calabrese,Bogdan I Iorga,Sheena E Radford,Seung-Hyun Cho,Han Remaut,Jean-François Collet

    The β-barrel assembly machinery (BAM) inserts outer membrane β-barrel proteins (OMPs) in the outer membrane of Gram-negative bacteria. In Enterobacteriacea, BAM also mediates export of the stress sensor lipoprotein RcsF to the cell surface by assembling RcsF–OMP complexes. Here, we report the crystal structure of the key BAM component BamA in complex with RcsF. BamA adopts an inward-open conformation

    更新日期:2020-06-23
  • Dissecting the Pol II transcription cycle and derailing cancer with CDK inhibitors.
    Nat. Chem. Biol. (IF 12.587) Pub Date : 2020-06-22
    Pabitra K Parua,Robert P Fisher

    Largely non-overlapping sets of cyclin-dependent kinases (CDKs) regulate cell division and RNA polymerase II (Pol II)-dependent transcription. Here we review the molecular mechanisms by which specific CDKs are thought to act at discrete steps in the transcription cycle and describe the recent emergence of transcriptional CDKs as promising drug targets in cancer. We emphasize recent advances in understanding

    更新日期:2020-06-23
  • Enzymes knuckle down to the job.
    Nat. Chem. Biol. (IF 12.587) Pub Date : 2020-06-17
    Paul H Walton

    A suite of new enzymes reveals more on how Nature breaks down plant-based polysaccharides and how these enzymes might be harnessed in the utilization of plant-based biomass.

    更新日期:2020-06-17
  • Unraveling proteasome engagement.
    Nat. Chem. Biol. (IF 12.587) Pub Date : 2020-06-15
    Cameron G Roberts,Jonathan N Pruneda

    A new study reveals that, in addition to its longstanding role in recruiting proteins to the proteasome, ubiquitination can also induce a structural destabilization that allows the target protein to be efficiently unraveled for degradation.

    更新日期:2020-06-15
  • In vitro prototyping and rapid optimization of biosynthetic enzymes for cell design.
    Nat. Chem. Biol. (IF 12.587) Pub Date : 2020-06-15
    Ashty S Karim,Quentin M Dudley,Alex Juminaga,Yongbo Yuan,Samantha A Crowe,Jacob T Heggestad,Shivani Garg,Tanus Abdalla,William S Grubbe,Blake J Rasor,David N Coar,Maria Torculas,Michael Krein,FungMin Eric Liew,Amy Quattlebaum,Rasmus O Jensen,Jeffrey A Stuart,Sean D Simpson,Michael Köpke,Michael C Jewett

    The design and optimization of biosynthetic pathways for industrially relevant, non-model organisms is challenging due to transformation idiosyncrasies, reduced numbers of validated genetic parts and a lack of high-throughput workflows. Here we describe a platform for in vitro prototyping and rapid optimization of biosynthetic enzymes (iPROBE) to accelerate this process. In iPROBE, cell lysates are

    更新日期:2020-06-15
  • Single-molecule analysis reveals agonist-specific dimer formation of µ-opioid receptors.
    Nat. Chem. Biol. (IF 12.587) Pub Date : 2020-06-15
    Jan Möller,Ali Isbilir,Titiwat Sungkaworn,Brendan Osberg,Christos Karathanasis,Vikram Sunkara,Eugene O Grushevskyi,Andreas Bock,Paolo Annibale,Mike Heilemann,Christof Schütte,Martin J Lohse

    G-protein-coupled receptors (GPCRs) are key signaling proteins that mostly function as monomers, but for several receptors constitutive dimer formation has been described and in some cases is essential for function. Using single-molecule microscopy combined with super-resolution techniques on intact cells, we describe here a dynamic monomer–dimer equilibrium of µ-opioid receptors (µORs), where dimer

    更新日期:2020-06-15
  • Author Correction: Higher-order epistasis shapes the fitness landscape of a xenobiotic-degrading enzyme.
    Nat. Chem. Biol. (IF 12.587) Pub Date : 2020-06-12
    Gloria Yang,Dave W Anderson,Florian Baier,Elias Dohmen,Nansook Hong,Paul D Carr,Shina Caroline Lynn Kamerlin,Colin J Jackson,Erich Bornberg-Bauer,Nobuhiko Tokuriki

    An amendment to this paper has been published and can be accessed via a link at the top of the paper.

    更新日期:2020-06-12
  • Use of paramagnetic 19F NMR to monitor domain movement in a glutamate transporter homolog.
    Nat. Chem. Biol. (IF 12.587) Pub Date : 2020-06-08
    Yun Huang,Xiaoyu Wang,Guohua Lv,Asghar M Razavi,Gerard H M Huysmans,Harel Weinstein,Clay Bracken,David Eliezer,Olga Boudker

    In proteins where conformational changes are functionally important, the number of accessible states and their dynamics are often difficult to establish. Here we describe a novel 19F-NMR spectroscopy approach to probe dynamics of large membrane proteins. We labeled a glutamate transporter homolog with a 19F probe via cysteine chemistry and with a Ni2+ ion via chelation by a di-histidine motif. We used

    更新日期:2020-06-08
  • Discovery of small-molecule enzyme activators by activity-based protein profiling.
    Nat. Chem. Biol. (IF 12.587) Pub Date : 2020-06-08
    Bernard P Kok,Srijana Ghimire,Woojoo Kim,Shreyosree Chatterjee,Tyler Johns,Seiya Kitamura,Jerome Eberhardt,Daisuke Ogasawara,Janice Xu,Ara Sukiasyan,Sean M Kim,Cristina Godio,Julia M Bittencourt,Michael Cameron,Andrea Galmozzi,Stefano Forli,Dennis W Wolan,Benjamin F Cravatt,Dale L Boger,Enrique Saez

    Activity-based protein profiling (ABPP) has been used extensively to discover and optimize selective inhibitors of enzymes. Here, we show that ABPP can also be implemented to identify the converse—small-molecule enzyme activators. Using a kinetically controlled, fluorescence polarization-ABPP assay, we identify compounds that stimulate the activity of LYPLAL1—a poorly characterized serine hydrolase

    更新日期:2020-06-08
  • Comparative tRNA sequencing and RNA mass spectrometry for surveying tRNA modifications.
    Nat. Chem. Biol. (IF 12.587) Pub Date : 2020-06-08
    Satoshi Kimura,Peter C Dedon,Matthew K Waldor

    Chemical modifications of the nucleosides that comprise transfer RNAs are diverse. However, the structure, location and extent of modifications have been systematically charted in very few organisms. Here, we describe an approach in which rapid prediction of modified sites through reverse transcription-derived signatures in high-throughput transfer RNA-sequencing (tRNA-seq) data is coupled with identification

    更新日期:2020-06-08
  • Publisher Correction: Complete reconstitution of the diverse pathways of gentamicin B biosynthesis.
    Nat. Chem. Biol. (IF 12.587) Pub Date : 2020-06-02
    Yeon Hee Ban,Myoung Chong Song,Jae-Yeon Hwang,Hea-Lyung Shin,Hak Joong Kim,Seung Kon Hong,Na Joon Lee,Je Won Park,Sun-Shin Cha,Hung-Wen Liu,Yeo Joon Yoon

    An amendment to this paper has been published and can be accessed via a link at the top of the paper.

    更新日期:2020-06-02
  • Rolf Huisgen (1920-2020).
    Nat. Chem. Biol. (IF 12.587) Pub Date : 2020-06-02
    Dirk Trauner

    Rolf Huisgen left us on 26 March 2020, just three months shy of his 100th birthday. His work has had an enormous influence on chemical biology, ranging from new methods for the synthesis of chemical probes to “click chemistry” and its application to in vivo bioconjugation.

    更新日期:2020-06-02
  • An allosteric modulator binds to a conformational hub in the β2 adrenergic receptor.
    Nat. Chem. Biol. (IF 12.587) Pub Date : 2020-06-01
    Xiangyu Liu,Jonas Kaindl,Magdalena Korczynska,Anne Stößel,Daniela Dengler,Markus Stanek,Harald Hübner,Mary J Clark,Jake Mahoney,Rachel Ann Matt,Xinyu Xu,Kunio Hirata,Brian K Shoichet,Roger K Sunahara,Brian K Kobilka,Peter Gmeiner

    Most drugs acting on G-protein-coupled receptors target the orthosteric binding pocket where the native hormone or neurotransmitter binds. There is much interest in finding allosteric ligands for these targets because they modulate physiologic signaling and promise to be more selective than orthosteric ligands. Here we describe a newly developed allosteric modulator of the β2-adrenergic receptor (β2AR)

    更新日期:2020-06-01
  • Site-specific ubiquitination affects protein energetics and proteasomal degradation.
    Nat. Chem. Biol. (IF 12.587) Pub Date : 2020-06-01
    Emma C Carroll,Eric R Greene,Andreas Martin,Susan Marqusee

    Changes in the cellular environment modulate protein energy landscapes to drive important biology, with consequences for signaling, allostery and other vital processes. The effects of ubiquitination are particularly important because of their potential influence on degradation by the 26S proteasome. Moreover, proteasomal engagement requires unstructured initiation regions that many known proteasome

    更新日期:2020-06-01
  • Selective N-glycan editing on living cell surfaces to probe glycoconjugate function.
    Nat. Chem. Biol. (IF 12.587) Pub Date : 2020-06-01
    Feng Tang,Mang Zhou,Ken Qin,Wei Shi,Ansor Yashinov,Yang Yang,Liyun Yang,Dongliang Guan,Lei Zhao,Yubo Tang,Yujie Chang,Lifen Zhao,Huaiyu Yang,Hu Zhou,Ruimin Huang,Wei Huang

    Cell surfaces are glycosylated in various ways with high heterogeneity, which usually leads to ambiguous conclusions about glycan-involved biological functions. Here, we describe a two-step chemoenzymatic approach for N-glycan-subtype-selective editing on the surface of living cells that consists of a first ‘delete’ step to remove heterogeneous N-glycoforms of a certain subclass and a second ‘insert’

    更新日期:2020-06-01
  • Pyridoxal-5'-phosphate-dependent alkyl transfer in nucleoside antibiotic biosynthesis.
    Nat. Chem. Biol. (IF 12.587) Pub Date : 2020-06-01
    Zheng Cui,Jonathan Overbay,Xiachang Wang,Xiaodong Liu,Yinan Zhang,Minakshi Bhardwaj,Anke Lemke,Daniel Wiegmann,Giuliana Niro,Jon S Thorson,Christian Ducho,Steven G Van Lanen

    Several nucleoside antibiotics are structurally characterized by a 5″-amino-5″-deoxyribose (ADR) appended via a glycosidic bond to a high-carbon sugar nucleoside (5′S,6′S)-5′-C-glycyluridine (GlyU). GlyU is further modified with an N-alkylamine linker, the biosynthetic origin of which has yet to be established. By using a combination of feeding experiments with isotopically labeled precursors and characterization

    更新日期:2020-06-01
  • Identification of a potent and selective covalent Pin1 inhibitor.
    Nat. Chem. Biol. (IF 12.587) Pub Date : 2020-06-01
    Benika J Pinch,Zainab M Doctor,Behnam Nabet,Christopher M Browne,Hyuk-Soo Seo,Mikaela L Mohardt,Shingo Kozono,Xiaolan Lian,Theresa D Manz,Yujin Chun,Shin Kibe,Daniel Zaidman,Dina Daitchman,Zoe C Yeoh,Nicholas E Vangos,Ezekiel A Geffken,Li Tan,Scott B Ficarro,Nir London,Jarrod A Marto,Stephen Buratowski,Sirano Dhe-Paganon,Xiao Zhen Zhou,Kun Ping Lu,Nathanael S Gray

    Peptidyl-prolyl cis/trans isomerase NIMA-interacting 1 (Pin1) is commonly overexpressed in human cancers, including pancreatic ductal adenocarcinoma (PDAC). While Pin1 is dispensable for viability in mice, it is required for activated Ras to induce tumorigenesis, suggesting a role for Pin1 inhibitors in Ras-driven tumors, such as PDAC. We report the development of rationally designed peptide inhibitors

    更新日期:2020-06-01
  • PGE1 and PGA1 bind to Nurr1 and activate its transcriptional function.
    Nat. Chem. Biol. (IF 12.587) Pub Date : 2020-05-25
    Sreekanth Rajan,Yongwoo Jang,Chun-Hyung Kim,Woori Kim,Hui Ting Toh,Jeha Jeon,Bin Song,Aida Serra,Julien Lescar,Jun Yeob Yoo,Serap Beldar,Hong Ye,Congbao Kang,Xue-Wei Liu,Melissa Feitosa,Yeahan Kim,Dabin Hwang,Geraldine Goh,Kah-Leong Lim,Hye Min Park,Choong Hwan Lee,Sungwhan F Oh,Gregory A Petsko,Ho Sup Yoon,Kwang-Soo Kim

    The orphan nuclear receptor Nurr1 is critical for the development, maintenance and protection of midbrain dopaminergic (mDA) neurons. Here we show that prostaglandin E1 (PGE1) and its dehydrated metabolite, PGA1, directly interact with the ligand-binding domain (LBD) of Nurr1 and stimulate its transcriptional function. We also report the crystallographic structure of Nurr1-LBD bound to PGA1 at 2.05 Å

    更新日期:2020-05-25
  • m6A-binding YTHDF proteins promote stress granule formation.
    Nat. Chem. Biol. (IF 12.587) Pub Date : 2020-05-25
    Ye Fu,Xiaowei Zhuang

    Diverse RNAs and RNA-binding proteins form phase-separated, membraneless granules in cells under stress conditions. However, the role of the prevalent mRNA methylation, m6A, and its binding proteins in stress granule (SG) assembly remain unclear. Here, we show that m6A-modified mRNAs are enriched in SGs, and that m6A-binding YTHDF proteins are critical for SG formation. Depletion of YTHDF1/3 inhibits

    更新日期:2020-05-25
  • Structural insights into β-1,3-glucan cleavage by a glycoside hydrolase family.
    Nat. Chem. Biol. (IF 12.587) Pub Date : 2020-05-25
    Camila R Santos,Pedro A C R Costa,Plínio S Vieira,Sinkler E T Gonzalez,Thamy L R Correa,Evandro A Lima,Fernanda Mandelli,Renan A S Pirolla,Mariane N Domingues,Lucelia Cabral,Marcele P Martins,Rosa L Cordeiro,Atílio T Junior,Beatriz P Souza,Érica T Prates,Fabio C Gozzo,Gabriela F Persinoti,Munir S Skaf,Mario T Murakami

    The fundamental and assorted roles of β-1,3-glucans in nature are underpinned on diverse chemistry and molecular structures, demanding sophisticated and intricate enzymatic systems for their processing. In this work, the selectivity and modes of action of a glycoside hydrolase family active on β-1,3-glucans were systematically investigated combining sequence similarity network, phylogeny, X-ray crystallography

    更新日期:2020-05-25
  • Voices of chemical biology.
    Nat. Chem. Biol. (IF 12.587) Pub Date : 2020-05-22

    We asked a collection of chemical biologists, “What was the most exciting research achievement or technology innovation in chemical biology in the last five years?” and reveal some of the perspectives we received.

    更新日期:2020-05-22
  • Greatest hits.
    Nat. Chem. Biol. (IF 12.587) Pub Date : 2020-05-22

    We present a selection of papers published in Nature Chemical Biology over the past five years that reflect the diversity and excitement of chemical biology research.

    更新日期:2020-05-22
  • 15 years of Nature Chemical Biology.
    Nat. Chem. Biol. (IF 12.587) Pub Date : 2020-05-22

    Over the past decade and a half, chemical biology has crystallized as a discipline and extended its reach into new scientific areas, but the field’s greatest promise lies ahead.

    更新日期:2020-05-22
  • Chromatin as a key consumer in the metabolite economy.
    Nat. Chem. Biol. (IF 12.587) Pub Date : 2020-05-22
    Katharine L Diehl,Tom W Muir

    In eukaryotes, chromatin remodeling and post-translational modifications (PTMs) shape the local chromatin landscape to establish permissive and repressive regions within the genome, orchestrating transcription, replication, and DNA repair in concert with other epigenetic mechanisms. Though cellular nutrient signaling encompasses a huge number of pathways, recent attention has turned to the hypothesis

    更新日期:2020-05-22
  • A tail of CRY selectivity.
    Nat. Chem. Biol. (IF 12.587) Pub Date : 2020-05-22
    Julia Lara,Brian D Zoltowski

    The discovery of selective modulators of two Cryptochrome isoforms, CRY1 and CRY2, permits a deeper understanding of how circadian clock proteins impact diverse aspects of our daily 24-h rhythms and how this intersects with metabolic pathways relevant to disease.

    更新日期:2020-05-22
  • The developing toolkit of continuous directed evolution.
    Nat. Chem. Biol. (IF 12.587) Pub Date : 2020-05-22
    Mary S Morrison,Christopher J Podracky,David R Liu

    Continuous directed evolution methods allow the key steps of evolution-gene diversification, selection, and replication-to proceed in the laboratory with minimal researcher intervention. As a result, continuous evolution can find solutions much more quickly than traditional discrete evolution methods. Continuous evolution also enables the exploration of longer and more numerous evolutionary trajectories

    更新日期:2020-05-22
  • Yes (again) to local NO.
    Nat. Chem. Biol. (IF 12.587) Pub Date : 2020-05-22
    Emrah Eroglu,Thomas Michel,Wolfgang F Graier,Roland Malli

    A pair of fluorescent indicator-tagged DNA-duplex scaffolds permit assessments of nitric oxide (NO) production on cell surfaces and in intracellular networks. The application of these nanoprobes indicates formations of local NO signals that might conserve cancer cell integrity.

    更新日期:2020-05-22
  • Design of orthogonal regulatory systems for modulating gene expression in plants.
    Nat. Chem. Biol. (IF 12.587) Pub Date : 2020-05-18
    Michael S Belcher,Khanh M Vuu,Andy Zhou,Nasim Mansoori,Amanda Agosto Ramos,Mitchell G Thompson,Henrik V Scheller,Dominique Loqué,Patrick M Shih

    Agricultural biotechnology strategies often require the precise regulation of multiple genes to effectively modify complex plant traits. However, most efforts are hindered by a lack of characterized tools that allow for reliable and targeted expression of transgenes. We have successfully engineered a library of synthetic transcriptional regulators that modulate expression strength in planta. By leveraging

    更新日期:2020-05-18
  • Multiplexed GTPase and GEF biosensor imaging enables network connectivity analysis.
    Nat. Chem. Biol. (IF 12.587) Pub Date : 2020-05-18
    Daniel J Marston,Marco Vilela,Jaewon Huh,Jinqi Ren,Mihai L Azoitei,George Glekas,Gaudenz Danuser,John Sondek,Klaus M Hahn

    Here we generate fluorescence resonance energy transfer biosensors for guanine exchange factors (GEFs) by inserting a fluorescent protein pair in a structural 'hinge' common to many GEFs. Fluorescent biosensors can map the activation of signaling molecules in space and time, but it has not been possible to quantify how different activation events affect one another or contribute to a specific cell

    更新日期:2020-05-18
  • Plant terpenoid metabolism co-opts a component of the cell wall biosynthesis machinery.
    Nat. Chem. Biol. (IF 12.587) Pub Date : 2020-05-18
    Adam Jozwiak,Prashant D Sonawane,Sayantan Panda,Constantine Garagounis,Kalliope K Papadopoulou,Bekele Abebie,Hassan Massalha,Efrat Almekias-Siegl,Tali Scherf,Asaph Aharoni

    Glycosylation is one of the most prevalent molecular modifications in nature. Single or multiple sugars can decorate a wide range of acceptors from proteins to lipids, cell wall glycans and small molecules, dramatically affecting their activity. Here, we discovered that by 'hijacking' an enzyme of the cellulose synthesis machinery involved in cell wall assembly, plants evolved cellulose synthase-like

    更新日期:2020-05-18
  • Author Correction: Plasma membranes are asymmetric in lipid unsaturation, packing and protein shape.
    Nat. Chem. Biol. (IF 12.587) Pub Date : 2020-05-15
    J H Lorent,K R Levental,L Ganesan,G Rivera-Longsworth,E Sezgin,M Doktorova,E Lyman,I Levental

    An amendment to this paper has been published and can be accessed via a link at the top of the paper.

    更新日期:2020-05-15
  • A small molecule G6PD inhibitor reveals immune dependence on pentose phosphate pathway.
    Nat. Chem. Biol. (IF 12.587) Pub Date : 2020-05-11
    Jonathan M Ghergurovich,Juan C García-Cañaveras,Joshua Wang,Emily Schmidt,Zhaoyue Zhang,Tara TeSlaa,Harshel Patel,Li Chen,Emily C Britt,Marta Piqueras-Nebot,Mari Carmen Gomez-Cabrera,Agustín Lahoz,Jing Fan,Ulf H Beier,Hahn Kim,Joshua D Rabinowitz

    Glucose is catabolized by two fundamental pathways, glycolysis to make ATP and the oxidative pentose phosphate pathway to make reduced nicotinamide adenine dinucleotide phosphate (NADPH). The first step of the oxidative pentose phosphate pathway is catalyzed by the enzyme glucose-6-phosphate dehydrogenase (G6PD). Here we develop metabolite reporter and deuterium tracer assays to monitor cellular G6PD

    更新日期:2020-05-11
  • Structural and mechanistic insights into 5-lipoxygenase inhibition by natural products.
    Nat. Chem. Biol. (IF 12.587) Pub Date : 2020-05-11
    Nathaniel C Gilbert,Jana Gerstmeier,Erin E Schexnaydre,Friedemann Börner,Ulrike Garscha,David B Neau,Oliver Werz,Marcia E Newcomer

    Leukotrienes (LT) are lipid mediators of the inflammatory response that are linked to asthma and atherosclerosis. LT biosynthesis is initiated by 5-lipoxygenase (5-LOX) with the assistance of the substrate-binding 5-LOX-activating protein at the nuclear membrane. Here, we contrast the structural and functional consequences of the binding of two natural product inhibitors of 5-LOX. The redox-type inhibitor

    更新日期:2020-05-11
  • Discovery of a NAPE-PLD inhibitor that modulates emotional behavior in mice.
    Nat. Chem. Biol. (IF 12.587) Pub Date : 2020-05-11
    Elliot D Mock,Mohammed Mustafa,Ozge Gunduz-Cinar,Resat Cinar,Gavin N Petrie,Vasudev Kantae,Xinyu Di,Daisuke Ogasawara,Zoltan V Varga,Janos Paloczi,Cristina Miliano,Giulia Donvito,Annelot C M van Esbroeck,Anouk M F van der Gracht,Ioli Kotsogianni,Joshua K Park,Andrea Martella,Tom van der Wel,Marjolein Soethoudt,Ming Jiang,Tiemen J Wendel,Antonius P A Janssen,Alexander T Bakker,Colleen M Donovan,Laura

    N-acylethanolamines (NAEs), which include the endocannabinoid anandamide, represent an important family of signaling lipids in the brain. The lack of chemical probes that modulate NAE biosynthesis in living systems hamper the understanding of the biological role of these lipids. Using a high-throughput screen, chemical proteomics and targeted lipidomics, we report here the discovery and characterization

    更新日期:2020-05-11
  • A nucleotide-switch mechanism mediates opposing catalytic activities of Rel enzymes.
    Nat. Chem. Biol. (IF 12.587) Pub Date : 2020-05-11
    Hedvig Tamman,Katleen Van Nerom,Hiraku Takada,Niels Vandenberk,Daniel Scholl,Yury Polikanov,Johan Hofkens,Ariel Talavera,Vasili Hauryliuk,Jelle Hendrix,Abel Garcia-Pino

    Bifunctional Rel stringent factors, the most abundant class of RelA/SpoT homologs, are ribosome-associated enzymes that transfer a pyrophosphate from ATP onto the 3' of guanosine tri-/diphosphate (GTP/GDP) to synthesize the bacterial alarmone (p)ppGpp, and also catalyze the 3' pyrophosphate hydrolysis to degrade it. The regulation of the opposing activities of Rel enzymes is a complex allosteric mechanism

    更新日期:2020-05-11
  • TRUPATH, an open-source biosensor platform for interrogating the GPCR transducerome.
    Nat. Chem. Biol. (IF 12.587) Pub Date : 2020-05-04
    Reid H J Olsen,Jeffrey F DiBerto,Justin G English,Alexis M Glaudin,Brian E Krumm,Samuel T Slocum,Tao Che,Ariana C Gavin,John D McCorvy,Bryan L Roth,Ryan T Strachan

    G-protein-coupled receptors (GPCRs) remain major drug targets, despite our incomplete understanding of how they signal through 16 non-visual G-protein signal transducers (collectively named the transducerome) to exert their actions. To address this gap, we have developed an open-source suite of 14 optimized bioluminescence resonance energy transfer (BRET) Gαβγ biosensors (named TRUPATH) to interrogate

    更新日期:2020-05-04
  • Asymmetry across the membrane.
    Nat. Chem. Biol. (IF 12.587) Pub Date : 2020-05-04
    Maria Makarova,Dylan M Owen

    A combination of biochemical and biophysical techniques to document the asymmetric distribution of lipids, with a particular focus on the acyl tails, in mammalian cell membranes show that protein transmembrane domains are similarly asymmetric.

    更新日期:2020-05-04
  • Plasma membranes are asymmetric in lipid unsaturation, packing and protein shape.
    Nat. Chem. Biol. (IF 12.587) Pub Date : 2020-05-04
    J H Lorent,K R Levental,L Ganesan,G Rivera-Longsworth,E Sezgin,M Doktorova,E Lyman,I Levental

    A fundamental feature of cellular plasma membranes (PMs) is an asymmetric lipid distribution between the bilayer leaflets. However, neither the detailed, comprehensive compositions of individual PM leaflets nor how these contribute to structural membrane asymmetries have been defined. We report the distinct lipidomes and biophysical properties of both monolayers in living mammalian PMs. Phospholipid

    更新日期:2020-05-04
  • Structural basis for selectivity in a highly reducing type II polyketide synthase.
    Nat. Chem. Biol. (IF 12.587) Pub Date : 2020-05-04
    Danyao Du,Yohei Katsuyama,Masanobu Horiuchi,Shinya Fushinobu,Aochiu Chen,Tony D Davis,Michael D Burkart,Yasuo Ohnishi

    In type II polyketide synthases (PKSs), the ketosynthase-chain length factor (KS-CLF) complex catalyzes polyketide chain elongation with the acyl carrier protein (ACP). Highly reducing type II PKSs, represented by IgaPKS, produce polyene structures instead of the well-known aromatic skeletons. Here, we report the crystal structures of the Iga11-Iga12 (KS-CLF) heterodimer and the covalently cross-linked

    更新日期:2020-05-04
  • Know your neighbors.
    Nat. Chem. Biol. (IF 12.587) Pub Date : 2020-05-01
    Grant Miura

    更新日期:2020-05-01
  • An iron-sulfur grip.
    Nat. Chem. Biol. (IF 12.587) Pub Date : 2020-05-01
    Caitlin Deane

    更新日期:2020-05-01
  • A metabolic labeling method detects m6A transcriptome-wide at single base resolution.
    Nat. Chem. Biol. (IF 12.587) Pub Date : 2020-04-27
    Xiao Shu,Jie Cao,Mohan Cheng,Siying Xiang,Minsong Gao,Ting Li,Xiner Ying,Fengqin Wang,Yanan Yue,Zhike Lu,Qing Dai,Xiaolong Cui,Lijia Ma,Yizhen Wang,Chuan He,Xinhua Feng,Jianzhao Liu

    Transcriptome-wide mapping of N6-methyladenosine (m6A) at base resolution remains an issue, impeding our understanding of m6A roles at the nucleotide level. Here, we report a metabolic labeling method to detect mRNA m6A transcriptome-wide at base resolution, called 'm6A-label-seq'. Human and mouse cells could be fed with a methionine analog, Se-allyl-L-selenohomocysteine, which substitutes the methyl

    更新日期:2020-04-27
  • Antibody-free enzyme-assisted chemical approach for detection of N6-methyladenosine.
    Nat. Chem. Biol. (IF 12.587) Pub Date : 2020-04-27
    Ye Wang,Yu Xiao,Shunqing Dong,Qiong Yu,Guifang Jia

    The inert chemical property of RNA modification N6-methyladenosine (m6A) makes it very challenging to detect. Most m6A sequencing methods rely on m6A-antibody immunoprecipitation and cannot distinguish m6A and N6,2'-O-dimethyladenosine modification at the cap +1 position (cap m6Am). Although the two antibody-free methods (m6A-REF-seq/MAZTER-seq and DART-seq) have been developed recently, they are dependent

    更新日期:2020-04-27
  • Flavin doesn't put all oxygens in one basket.
    Nat. Chem. Biol. (IF 12.587) Pub Date : 2020-05-01
    David Leys,Nigel S Scrutton
    更新日期:2020-04-24
  • Anti-HIV agents inspired by antibodies.
    Nat. Chem. Biol. (IF 12.587) Pub Date : 2020-05-01
    Shan Su,Shibo Jiang
    更新日期:2020-04-24
  • How m6A sneaks into DNA.
    Nat. Chem. Biol. (IF 12.587) Pub Date : 2020-04-16
    Paolo Spingardi,Skirmantas Kriaucionis
    更新日期:2020-04-24
  • Short disordered protein segment regulates cross-species transmission of a yeast prion.
    Nat. Chem. Biol. (IF 12.587) Pub Date : 2020-04-13
    Toshinobu Shida,Yuji O Kamatari,Takao Yoda,Yoshiki Yamaguchi,Michael Feig,Yumiko Ohhashi,Yuji Sugita,Kazuo Kuwata,Motomasa Tanaka
    更新日期:2020-04-24
Contents have been reproduced by permission of the publishers.
导出
全部期刊列表>>
胸部和胸部成像专题
自然科研论文编辑服务
ACS ES&T Engineering
ACS ES&T Water
屿渡论文,编辑服务
鲁照永
华东师范大学
苏州大学
南京工业大学
南开大学
中科大
唐勇
跟Nature、Science文章学绘图
隐藏1h前已浏览文章
中洪博元
课题组网站
新版X-MOL期刊搜索和高级搜索功能介绍
ACS材料视界
x-mol收录
广东实验室
南京大学
王杰
南科大
刘尊峰
湖南大学
清华大学
王小野
中山大学化学工程与技术学院
试剂库存
天合科研
down
wechat
bug