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Phenylalanine metabolism-dependent lignification confers rhizobacterium-induced plant resistance Plant Physiol. (IF 6.5) Pub Date : 2025-02-14 Qi Li, Zhuangzhuang Liu, Zexuan Jiang, Mingyun Jia, Zhaoqi Hou, Daolong Dou, Jinping Yu
Phenylalanine metabolism serves as an important route for production of diverse secondary metabolites including phenylpropanoids. The phenylpropanoid pathway is involved in plant immunity, but whether it can regulate rhizobacteria-induced resistance is poorly understood. In this study, we confirmed a growth-promoting rhizobacterium strain JR48 could induce resistance, strengthen salicylic acid (SA)
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Arabidopsis accessions and their difference in heat tolerance during meiosis. Plant Physiol. (IF 6.5) Pub Date : 2025-02-12 Joke De Jaeger-Braet
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Atomic force microscopy imaging of plant cell walls Plant Physiol. (IF 6.5) Pub Date : 2025-02-10 Junbao Pu, Jie Ma, Hang Zhai, Shanshan Wu, Youmei Wang, Christine V Putnis, Lijun Wang, Wenjun Zhang
Plant cell walls are highly dynamic, complex structures composed of multiple biopolymers that form a scaffold surrounding the plant cell. A nanoscale understanding of their architecture, mechanical properties, and formation/degradation dynamics is crucial for revealing structure–function relationships, mechanisms of shape formation, and cell development. Although imaging techniques have been extensively
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Leaf minimum conductance dynamics during and after heat stress: Implications for plant survival under hotter droughts Plant Physiol. (IF 6.5) Pub Date : 2025-02-10 Viviane de Araújo Brito Fernandes, Fernanda Santos Farnese, Brenner Ryan Arantes, Maria Lúcia Fontineles da Silva, Fabiano Guimarães Silva, José M Torres-Ruiz, Martijn Slot, Hervé Cochard, Paulo Eduardo Menezes-Silva
Exposure to temperatures above a critical threshold (temperature of phase transition, Tp) can damage the leaf cuticle, leading to increased leaf minimum conductance (gleaf-res). Despite the implications of increased gleaf-res for species survival under hotter-drought conditions, little is known about the dynamics of gleaf-res variation after heatwave episodes. Here, we examined the gleaf-res variation
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Ceramide-1-phosphate enhances defense responses against Sclerotinia sclerotiorum in Brassica napus Plant Physiol. (IF 6.5) Pub Date : 2025-02-10 Zhewen Ouyang, Zengdong Tan, Usman Ali, Ying Zhang, Bo Li, Xuan Yao, Bao Yang, Liang Guo
Sclerotinia stem rot caused by Sclerotinia sclerotiorum is one of the most severe diseases affecting the growth and production of Brassica napus. Sphingolipid metabolism plays a crucial role in plant response to pathogens. In this study, we show that ceramide kinase (CERK) is significantly induced during S. sclerotiorum infection to produce higher levels of ceramide-1-phosphate (C1P) in B. napus. The
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Plant PI-PLC signaling in stress and development Plant Physiol. (IF 6.5) Pub Date : 2025-02-10 Ana M Laxalt, Max van Hooren, Teun Munnik
Phosphoinositide-specific phospholipase C (PI-PLC) signaling is involved in various plant stress and developmental responses. Though several aspects of this lipid signaling pathway are conserved within animals and plants, clear differences have also emerged. While animal PLC signaling is characterized by the hydrolysis of PIP2 and production of IP3 and DAG as second messengers to activate Ca2+ and
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Correction to: The transcriptional integration of environmental cues with root cell type development. Plant Physiol. (IF 6.5) Pub Date : 2025-02-07
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Mixed-planting: A useful tool to build climate-resilient forests. Plant Physiol. (IF 6.5) Pub Date : 2025-02-07 Hannah M McMillan
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When the CAT wants to play: The role of interaction between CRCK3 and CAT2 in Arabidopsis salt stress tolerance. Plant Physiol. (IF 6.5) Pub Date : 2025-02-05 Sara Selma
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Modifying the TL1-BINDING FACTOR upstream open reading frame coordinates plant growth and defense in rice Plant Physiol. (IF 6.5) Pub Date : 2025-02-03 Jingjing Tian, Yingshuang Liu, Dan Chen, Guoyong Xu, Meng Yuan
Redesigning upstream open reading frames could fine-tune target gene translation, thereby facilitating an optimal balance between plant growth and defense.
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Enhanced storage lipid biosynthesis promotes somatic embryogenesis in citrus Plant Physiol. (IF 6.5) Pub Date : 2025-01-31 Erlin Gao, Yunju Zhao, Mengxia Wu, Xiaolu Qu, Xiaomeng Wu, Wenwu Guo, Pengwei Wang
A strong association between somatic embryogenesis competence and lipid accumulation in the embryogenic callus of citrus may facilitate plant regeneration and germplasm utilization.
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ATP-CITRATE LYASEB1 supplies materials for sporopollenin biosynthesis and microspore development in Arabidopsis Plant Physiol. (IF 6.5) Pub Date : 2025-01-31 Chang-Kai Ma, Sheng-Hong Wang, Qiang-Sheng Shi, Meng-Die Guo, Yan-Ming Yang, Jia Fu, Xiao Chen, Yi-Chen Mao, Xuehui Huang, Jun Zhu, Zhong-Nan Yang
Acetyl-CoA is the main substrate of lipid metabolism and functions as an energy source for plant development. In the cytoplasm, acetyl-CoA is mainly produced by ATP-citrate lyase (ACL), which is composed of ACLA and ACLB subunits. In this study, we isolated the restorer-4 (res4) of the thermosensitive genic male sterile mutant reversible male sterile-2 (rvms-2) in Arabidopsis (Arabidopsis thaliana)
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At5g63290 does not encode coproporphyrinogen III oxidase Plant Physiol. (IF 6.5) Pub Date : 2025-01-30 Wenjuan Ji, Huijuan Wang, An Ning, Xuan Zhou, Bingxiao Wen, Bernhard Grimm, Zhenhua Liu
At5g63290 is not responsible for the decarboxylation of coproporphyrinogen III, prompting a revision of the tetrapyrrole biosynthesis pathway in Arabidopsis thaliana.
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Surviving Floods: Escape and Quiescence Strategies of Rice Coping with Submergence Plant Physiol. (IF 6.5) Pub Date : 2025-01-30 Motoyuki Ashikari, Keisuke Nagai, Julia Bailey-Serres
Historical and recent insights into the molecular mechanisms of escape and quiescence strategies employed by rice to survive flooding.
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Destined for destruction: The role of methionine aminopeptidases and plant cysteine oxidases in N-degron formation Plant Physiol. (IF 6.5) Pub Date : 2025-01-28 Andrea Fuentes-Terrón, Rebecca Latter, Samuel Madden, Isabel Manrique-Gil, Jessenia Estrada, Noelia Arteaga, Inmaculada Sánchez-Vicente, Oscar Lorenzo, Emily Flashman
The cysteine/arginine (Cys/Arg) branch of the N-degron pathway controls the stability of certain proteins with methionine (Met)-Cys N-termini, initiated by Met cleavage and Cys oxidation. In seeding plants, target proteins include the Group VII Ethylene Response Factors, which initiate adaptive responses to low oxygen (hypoxic) stress, as well as Vernalization 2 (VRN2) and Little Zipper 2 (ZPR2), which
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Inhibition of mitochondrial energy production leads to reorganization of the plant endomembrane system Plant Physiol. (IF 6.5) Pub Date : 2025-01-28 Taotao Wang, Xinjing Li, Hongying Yu, Heng Zhang, Zhiping Xie, Qingqiu Gong
Mitochondria have generated the bulk of ATP to fuel cellular activities, including membrane trafficking, since the beginning of eukaryogenesis. How inhibition of mitochondrial energy production will affect the form and function of the endomembrane system and whether such changes are specific in today’s cells remain unclear. Here, we treated Arabidopsis thaliana with antimycin A (AA), a potent inhibitor
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Isopropylmalate synthase regulatory domain removal abolishes feedback regulation at the expense of leucine homeostasis in plants Plant Physiol. (IF 6.5) Pub Date : 2025-01-27 Mohan Varghese, Roshan Kumar, Aprajita Sharma, Asif Lone, Jonathan Gershenzon, Naveen C Bisht
In the leucine (Leu) biosynthesis pathway, homeostasis is achieved through a feedback regulatory mechanism facilitated by the binding of the end-product Leu at the C-terminal regulatory domain of the first committed enzyme, isopropylmalate synthase (IPMS). In vitro studies have shown that removing the regulatory domain abolishes the feedback regulation on plant IPMS while retaining its catalytic activity
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ELONGATED HYPOCOTYL 5 promotes root growth by maintaining redox homeostasis and repressing oxidative stress response Plant Physiol. (IF 6.5) Pub Date : 2025-01-27 Xinxing Zhu, Lin Liu, Liyun Yang, Fei Ma, Ting Yang, Jing Fu, Hongchang Cui
Oxidative stress is a major threat to plant growth and survival. To understand how plants cope with oxidative stress, we carried out a genetic screen for Arabidopsis (Arabidopsis thaliana) mutants with altered response to hydrogen peroxide (H2O2) in root growth. Herein, we report the characterization of one of the hypersensitive mutants obtained. This mutant had slightly shorter roots in normal growth
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Nitro-fatty acids modulate germination onset through S-nitrosothiol metabolism Plant Physiol. (IF 6.5) Pub Date : 2025-01-25 Capilla Mata-Pérez, Juan C Begara-Morales, María N Padilla, Mounira Chaki, Beatriz Sánchez-Calvo, Alfonso Carreras, Lorena Aranda-Caño, Manuel Melguizo, Raquel Valderrama, Inmaculada Sánchez-Vicente, Óscar Lorenzo, Juan B Barroso
Nitro-fatty acids (NO2-FAs) have emerged as key components of nitric oxide (NO) signalling in eukaryotes. We previously described how nitro-linolenic acid (NO2-Ln), the major NO2-FA detected in plants, regulates S-nitrosoglutathione (GSNO) levels in Arabidopsis (Arabidopsis thaliana). However, the underlying molecular mechanisms remain undefined. Here, we used a combination of physiological, biochemical
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LACCASE35 Enhances Lignification and Resistance Against Pseudomonas syringae pv. actinidiae Infection in Kiwifruit Plant Physiol. (IF 6.5) Pub Date : 2025-01-24 Yawei Li, Dongle Zhang, Xiaojie Wang, Fuxi Bai, Rui Li, Rongrong Zhou, Shunyuan Wu, Zemin Fang, Wei Liu, Lili Huang, Pu Liu
Kiwifruit bacterial canker, a highly destructive disease caused by Pseudomonas syringae pv. actinidiae (Psa), seriously affects kiwifruit (Actinidia spp.) production. Lignin deposition in infected cells serves as a defense mechanism, effectively suppressing pathogen growth. However, the underlying process remains unclear. In this study, we determined that Psa infection leads to a significant increase
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Phosphorylation-dependent VaMYB4a regulates cold stress in grapevine by inhibiting VaPIF3 and activating VaCBF4 Plant Physiol. (IF 6.5) Pub Date : 2025-01-24 Qinhan Yu, Qiaoling Zheng, Chang Liu, Junxia Zhang, Yaping Xie, Wenkong Yao, Jiaxin Li, Ningbo Zhang, Xinyi Hao, Weirong Xu
Cold stress severely impacts the quality and yield of grapevine (Vitis L.). In this study, we extend our previous work to elucidate the role and regulatory mechanisms of Vitis amurensis MYB transcription factor 4a (VaMYB4a) in grapevine's response to cold stress. Our results identified VaMYB4a as a key positive regulator of cold stress. We demonstrated that VaMYB4a undergoes phosphorylation by V. amurensis
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The translation initiation factor eIF3M2 upregulates HEAT SHOCK PROTEIN 70 to maintain pollen tube membrane integrity during heat shock Plant Physiol. (IF 6.5) Pub Date : 2025-01-24 Zahra Kahrizi, Christos Michailidis, Karel Raabe, Vinod Kumar, David Honys, Said Hafidh
Pollen germination and pollen tube (PT) growth are extremely sensitive to high temperatures. During heat stress (HS), global translation shuts down and favors the maintenance of the essential cellular proteome for cell viability and protection against protein misfolding. Here, we demonstrate that under normal conditions, the Arabidopsis (Arabidopsis thaliana) eukaryotic translation initiation factor
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A C4 plant K+ channel accelerates stomata to enhance C3 photosynthesis and water use efficiency Plant Physiol. (IF 6.5) Pub Date : 2025-01-24 Fernanda A L Silva-Alvim, Jonas Chaves Alvim, Julian M Hibberd, Andrew R Harvey, Michael R Blatt
Accelerating stomatal kinetics through synthetic optogenetics and mutations that enhance guard cell K+ flux has proven a viable strategy to improve water use efficiency and biomass production. Stomata of the model C4 species Gynandropsis gynandra, a relative of the C3 plant Arabidopsis thaliana, are similarly fast to open and close. We identified and cloned the guard cell rectifying outward K+ channel
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The ASPARAGINE-RICH PROTEIN–LYST-INTERACTING PROTEIN5 complex regulates non-canonical AUTOPHAGY8 degradation in Arabidopsis Plant Physiol. (IF 6.5) Pub Date : 2025-01-24 Yanying Wu, Kaikai Zhu, Si Chen, Enzhen Xing, Jiajia Li, Wenqi Tian, Ming Gao, Jiaxin Kong, Danni Zheng, Xue Wang, Weihong Zhou, Shuzhen Men, Xinqi Liu
The endocytic and autophagic pathways play important roles in abiotic stress responses and maintaining cellular homeostasis in plants. Asparagine Rich Proteins (NRPs) are plant-specific stress-responsive proteins that are involved in many abiotic stress-related signaling pathways. We previously demonstrated that NRP promotes PIN FORMED 2 (PIN2) vacuolar degradation to maintain PIN2 homeostasis under
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Sphingolipid remodeling in the plasma membrane is essential for osmotic stress tolerance in Arabidopsis Plant Physiol. (IF 6.5) Pub Date : 2025-01-23 Yong-Kang Li, Yu-Meng Zhang, Guang-Yi Dai, Yi-Li Chen, Ding-Kang Chen, Nan Yao
Osmotic stress caused by drought, salinity, or cold conditions is an important abiotic factor that decreases membrane integrity and causes cell death, thus decreasing plant growth and productivity. Remodeling cell membrane composition via lipid turnover can counter the loss of membrane integrity and cell death caused by osmotic stress. Sphingolipids are important components of eukaryotic membrane systems;
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Toll/interleukin-1 receptor-only genes contribute to immune responses in maize Plant Physiol. (IF 6.5) Pub Date : 2025-01-23 Qiang Zhang, Derong Gao, Lei Tian, Kirstin Feussner, Bin Li, Long Yang, Qin Yang, Yuelin Zhang, Xin Li, Ivo Feussner, Fang Xu
Proteins with Toll/interleukin-1 receptor (TIR) domains are widely distributed in both prokaryotes and eukaryotes, serving as essential components of immune signaling. Although monocots lack the major TIR-nucleotide-binding (NB)-leucine-rich repeat (LRR)-type (TNL) immune receptors, they possess a small number of TIR-only proteins, the function of which remains largely unknown. In the monocot maize
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BRASSINAZOLE RESISTANT 1 delays photoperiodic flowering by repressing CONSTANS transcription Plant Physiol. (IF 6.5) Pub Date : 2025-01-23 Xingwen Xu, Wenbo Jiang, Yangbo Chen, Hao Tian, Zijian Yang, Shuo Liu, Xiaopeng Li, Chunhui Song, Zhangli Ye, Wei Guo, Dongdong Kong, Congcong Hou, Legong Li, Liangyu Liu
Photoperiodic regulation of flowering time plays a critical role in plant reproductive success and crop yield. In Arabidopsis thaliana, the expression of the CONSTANS (CO) gene is closely regulated by day length and is modulated by both environmental and endogenous cues for precise control over flowering. Our findings reveal that the phytohormone brassinosteroid (BR) pathway represses flowering by
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Dynamic photosynthetic labeling and carbon-positional mass spectrometry monitor in vivo Rubisco carbon assimilation rates Plant Physiol. (IF 6.5) Pub Date : 2025-01-21 Yogeswari Rajarathinam, Luisa Wittemeier, Kirstin Gutekunst, Martin Hagemann, Joachim Kopka
RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE/OXYGENASE (RUBISCO) is the most abundant enzyme and CO2 bio-sequestration system on Earth. Its in vivo activity is usually determined by 14CO2 incorporation into 3-phosphoglycerate (3PGA). However, the radiometric analysis of 3PGA does not distinguish carbon positions. Hence, RUBISCO activity that fixes carbon into the 1-C position of 3PGA and Calvin–Benson–Bassham
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The actin cytoskeleton regulates danger-associated molecular pattern signaling and PEP1 RECEPTOR1 internalization Plant Physiol. (IF 6.5) Pub Date : 2025-01-17 Hongping Qian, Xinxiu Zuo, Yi Man, Changwen Xu, Pengyun Luo, Lijuan Yao, Ruohan Geng, Binghe Wang, Shihui Niu, Jinxing Lin, Yaning Cui
In plants, cytoskeletal proteins assemble into dynamic polymers that play numerous roles in diverse fundamental cellular processes, including endocytosis, vesicle trafficking, and the spatial distribution of organelles and protein complexes. Plant elicitor peptides (Peps) are damage/danger-associated molecular patterns (DAMPs) that are perceived by the receptor-like kinases PEP RECEPTOR 1 (PEPR1) and
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PROCERA interacts with JACKDAW in gibberellin-enhanced source-sink sucrose partitioning in tomato Plant Physiol. (IF 6.5) Pub Date : 2025-01-17 Yufei Liang, Jingyi Zhao, Rui Yang, Jiayu Bai, Wanxing Hu, Lixia Gu, Zhaoyuan Lian, Heqiang Huo, Jia Guo, Haijun Gong
Proper regulation of the source-sink relationship is an effective way to increase crop yield. Gibberellin (GA) is an important regulator of plant growth and development, and physiological evidence has demonstrated that GA can promote source-sink sucrose partitioning. However, the underlying molecular mechanism remains unclear. Here, we used a combination of physiological and molecular approaches to
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The vacuolar phosphatases PAP26 and HIIA2.1 hydrolyze 5’-, 3’-, and 2’-nucleotides derived from RNA degradation Plant Physiol. (IF 6.5) Pub Date : 2025-01-17 Nabila Firdoos, Lukas Krumwiede, Nieves Medina Escobar, Leonie Treichel, Lisa Fischer, Marco Herde, Claus-Peter Witte
The vacuole is an important site for RNA degradation. Autophagy delivers RNA to the vacuole, where the vacuolar T2 RNase Ribonuclease 2 (RNS2) plays a major role in RNA catabolism. The presumed products of RNS2 activity are 3’-nucleoside monophosphates (3’-NMPs). Vacuolar phosphatases that carry out 3’-NMP hydrolysis are required to metabolize 3’-NMPs, but the specific players remain unknown. Using
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Fatty acid desaturase 3-mediated α-linolenic acid biosynthesis in plants Plant Physiol. (IF 6.5) Pub Date : 2025-01-17 Mohammad Fazel Soltani Gishini, Pradeep Kachroo, David Hildebrand
Omega (ω)-3 fatty acids (FAs) are essential components of cell membranes that also serve as precursors of numerous regulatory molecules. α-linolenic acid (ALA), one of the most important ω3 FAs in plants, is synthesized in both the plastid and extraplastidial compartments. FA Desaturase (FAD) 3 is an extraplastidial enzyme that converts linoleic acid (LA) to ALA. Phylogenetic analysis suggested that
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The transcription factor GmFULc regulates soybean plant height by binding the promoter of a gibberellin-responsive gene Plant Physiol. (IF 6.5) Pub Date : 2025-01-17 Jingzhe Sun, Xiaoming Zhang, Junhang Feng, Xiaofei Ma, Yujia Ji, Shujun Chen, Jihui Li, Dongmei Li, Xiujun Wang, Lin Zhao
Plant height is a crucial agronomic characteristic that substantially influences soybean [Glycine max (L.) Merr.] yield. FRUITFULLc (GmFULc) is a MADS-box transcription factor that acts as a growth promoter in soybean; however, the mechanism by which GmFULc regulates soybean height is unknown. This study revealed that GmFULc:GmFULc (the expression of the GmFULc gene driven by its native promoter) soybeans
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The transcription factor SlLBD40 regulates seed germination by inhibiting cell wall remodeling enzymes during endosperm weakening Plant Physiol. (IF 6.5) Pub Date : 2025-01-17 Jialong Zhang, Lun Liu, Danhui Dong, Jiayi Xu, Hongxin Li, Qilin Deng, Yan Zhang, Wei Huang, Haijun Zhang, Yang-Dong Guo
Uniform seed germination is crucial for consistent seedling emergence and efficient seedling production. In this study, we identified a seed-expressed protein in tomato (Solanum lycopersicum), lateral organ boundaries domain 40 (SlLBD40), that regulates germination speed. CRISPR/Cas9-generated SlLBD40 knockout mutants exhibited faster germination due to enhanced seed imbibition, independent of the
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Metabolic modeling suggested noncanonical algal carbon concentrating mechanism in Cyanidioschyzon merolae. Plant Physiol. (IF 6.5) Pub Date : 2025-02-07 Maneesh Lingwan
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Born of frustration: the emergence of Camelina sativa as a platform for lipid biotechnology. Plant Physiol. (IF 6.5) Pub Date : 2025-01-15 Richard P Haslam, Louise V Michaelson, Peter J Eastmond, Johnathan A Napier
The emerging crop Camelina sativa (L.) Crantz (camelina) is a Brassicaceae oilseed with a rapidly growing reputation for the deployment of advanced lipid biotechnology and metabolic engineering. Camelina is recognised by agronomists for its traits including yield, oil/protein content, drought tolerance, limited input requirements, plasticity and resilience. Its utility as a platform for metabolic engineering
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The MADS-RIPENING INHIBITOR–DIVARICATA1 module regulates carotenoid biosynthesis in nonclimacteric Capsicum fruits Plant Physiol. (IF 6.5) Pub Date : 2025-01-11 Yinggang Wang, Xinhui Li, Huixia Qiu, Ruting Chen, Aisheng Xiong, Zhisheng Xu, Wu Miao, Rugang Chen, Peizhi Wang, Xilin Hou, Huiyang Yu, Bozhi Yang, Sha Yang, Huan Suo, Xuexiao Zou, Zhoubin Liu, Lijun Ou
Carotenoids play indispensable roles in the ripening process of fleshy fruits. Capsanthin is a widely distributed and utilized natural red carotenoid. However, the regulatory genes involved in capsanthin biosynthesis remain insufficient. Here, we identified the MADS-box transcription factor RIPENING INHIBITOR (MADS-RIN) in pepper (Capsicum annuum), which regulates ripening in climacteric tomato (Solanum
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Protein phosphatase PP2C2 dephosphorylates transcription factor ZAT5 and modulates tomato fruit ripening Plant Physiol. (IF 6.5) Pub Date : 2025-01-11 Yafei Li, Yanan Chang, Yiran Wang, Chaolin Gan, Chonghua Li, Xuejun Zhang, Yang-Dong Guo, Na Zhang
Although C2H2 zinc finger transcription factors are important in plant growth, development, and stress resistance, their specific roles in fruit ripening have been less explored. Here, we demonstrate that the C2H2 zinc finger transcription factor 5 (SlZAT5) regulates fruit ripening in tomato (Solanum lycopersicum L.). Overexpression of SlZAT5 delayed ripening, while its knockout accelerated it, confirming
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Chromatin Accessibility Mediated by CHROMATIN REMODELING 11 Promotes Chilling Tolerance in Rice Plant Physiol. (IF 6.5) Pub Date : 2025-01-11 Jing Li, He Liu, Hanying Qian, Shan Lu, Yufeng Wu, Jian Hua, Baohong Zou
Chromatin remodeling plays a crucial role in controlling gene transcription by modifying chromatin structure. However, the involvement of chromatin remodeling in plant stress responses, especially cold tolerance, through chromatin accessibility remains largely unexplored. Here, we report that rice (Oryza sativa L.)CHROMATIN REMODELING 11 (OsCHR11) positively regulates chilling tolerance by enhancing
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Cycling Dof Factor 3 mediates light-dependent ascorbate biosynthesis by activating GDP-L-galactose phosphorylase in Rosa roxburghii fruit Plant Physiol. (IF 6.5) Pub Date : 2025-01-11 Qianmin Huang, Yali Yan, Xue Zhang, Xuejiao Cao, Richard Ludlow, Min Lu, Huaming An
Light plays an important role in determining the L-ascorbate (AsA) pool size in plants, primarily through the transcriptional regulation of AsA metabolism-related genes. However, the specific mechanism of transcriptional induction responsible for light-dependent AsA biosynthesis remains unclear. In this study, we used a promoter sequence containing light-responsive motifs from GDP-L-galactose phosphorylase
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UBIQUITIN-CONJUGATING ENZYME34 mediates pyrophosphatase AVP1 turnover and regulates abiotic stress responses in Arabidopsis Plant Physiol. (IF 6.5) Pub Date : 2025-01-11 Qiyu Xu, Xingjian Zhang, Ruifeng Zhao, Shengjun Li, Johannes Liesche
Understanding the molecular mechanisms of abiotic stress responses in plants is instrumental for the development of climate-resilient crops. Key factors in abiotic stress responses, such as the proton- pumping pyrophosphatase (AVP1), have been identified, but their function and regulation remain elusive. Here, we explored the post-translational regulation of AVP1 by the ubiquitin-conjugating enzyme
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Go with the flow: ABCC4 mediates cytokinin efflux to control root development. Plant Physiol. (IF 6.5) Pub Date : 2025-02-07 Héctor H Torres-Martínez
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Branched oligosaccharides cause atypical starch granule initiation in Arabidopsis chloroplasts Plant Physiol. (IF 6.5) Pub Date : 2025-01-09 Arvid J M Heutinck, Selina Camenisch, Michaela Fischer-Stettler, Mayank Sharma, Barbara Pfister, Simona Eicke, Chun Liu, Samuel C Zeeman
Plant chloroplasts store starch during the day, which acts as a source of carbohydrates and energy at night. Starch granule initiation relies on the elongation of malto-oligosaccharide primers. In Arabidopsis thaliana, PROTEIN TARGETING TO STARCH 2 (PTST2) and STARCH SYNTHASE 4 (SS4) are essential for the selective binding and elongation of malto-oligosaccharide primers, respectively, and very few
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Paving the way to secondary dormancy: mind the DOG's tail. Plant Physiol. (IF 6.5) Pub Date : 2025-02-07 Dechang Cao
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Hot to Go: The impact of protein nitrosylation on plant fertility. Plant Physiol. (IF 6.5) Pub Date : 2025-02-07 Anna Moseler
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How plants adapt to combined and sequential abiotic stresses: A transcriptomics approach. Plant Physiol. (IF 6.5) Pub Date : 2024-12-24 Burcu Alptekin,Alicja B Kunkowska
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Natural Variation in a Molybdate Transporter Confers Salt Tolerance in Tomato Plant Physiol. (IF 6.5) Pub Date : 2025-01-07 Zhen Wang, Yechun Hong, Zhaojun Guo, Dianjue Li, Zhen-Fei Chao, Guangtao Zhu, Jian-Kang Zhu
Natural variation in a molybdate transporter-encoding gene is associated with molybdenum accumulation, which reduces hypocotyl growth and improves salt tolerance in tomato.
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A benzoxazinoid twist to boron homeostasis story in maize. Plant Physiol. (IF 6.5) Pub Date : 2024-12-23 Janlo M Robil,Henryk Straube,Thu M Tran
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The NAC transcription factor LpNAC48 promotes trichome formation in Lilium pumilum Plant Physiol. (IF 6.5) Pub Date : 2025-01-06 Yin Xin, Wenqiang Pan, Yajie Zhao, Chenglong Yang, Jingru Li, Shaokun Wang, Jingxiang Wu, Mingfang Zhang, Jinxin Shi, Yang Ma, Shaozhong Fang, Yuwei Liang, Michele Zaccai, Xiuhai Zhang, Yunpeng Du, Jian Wu
Trichomes play a crucial role in plant resistance to abiotic and biotic stresses, and their development and characteristics vary across different species. This study demonstrates that trichomes of Lilium pumilum exhibit synchronized growth during flower bud differentiation and enhance the plant's adaptability to UV-B radiation and aphid infection. We identified LpNAC48, a NAC family transcription factor
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PHENYLALANINE AMMONIA-LYASE 2 regulates secondary metabolism and confers manganese tolerance in Stylosanthes guianensis Plant Physiol. (IF 6.5) Pub Date : 2025-01-06 Linjie Wang, Jifu Li, Liting Liu, Rongshu Dong, Guodao Liu, Idupulapati M Rao, Zhijian Chen
Stylo (Stylosanthes guianensis) is a tropical legume that exhibits considerable tolerance to manganese (Mn) toxicity, which severely constrains plant growth in acidic soils. To elucidate the Mn detoxification mechanisms in stylo, this study investigated the excess Mn-regulated metabolic profile of stylo roots and examined the role of metabolic enzymes in Mn tolerance. Excess Mn triggered oxidative
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Take a Deep BReath: Manipulating brassinosteroid homeostasis helps cereals adapt to environmental stress Plant Physiol. (IF 6.5) Pub Date : 2025-01-06 Karolina Zolkiewicz, Damian Gruszka
Global climate change leads to the increased occurrence of environmental stress (including drought and heat stress) during the vegetative and reproductive stages of cereal crop development. Thus, more attention should be given to developing new cereal cultivars with improved tolerance to environmental stress. However, during the development of new stress-tolerant cereal cultivars, the balance between
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The Target of Rapamycin kinase is a positive regulator of plant fatty acid and lipid synthesis Plant Physiol. (IF 6.5) Pub Date : 2024-12-31 Hui Liu, Jantana Blanford, Hai Shi, Jorg Schwender, John Shanklin, Zhiyang Zhai
In eukaryotes, Target of Rapamycin (TOR), a conserved protein sensor kinase, integrates diverse environmental cues, including growth factor signals, energy availability, and nutritional status, to direct cell growth. In plants, TOR is activated by light and sugars and regulates a wide range of cellular processes, including protein synthesis and metabolism. Fatty acid synthesis is key to membrane biogenesis
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Bacillus amyloliquefaciens promotes cluster root formation of white lupin under low phosphorus by mediating auxin levels Plant Physiol. (IF 6.5) Pub Date : 2024-12-25 Jinyong Yang, Shenglan Li, Xiangxue Zhou, Chongxuan Du, Ju Fang, Xing Li, Jun Zhao, Fan Ding, Yue Wang, Qian Zhang, Zhengrui Wang, Jianping Liu, Gangqiang Dong, Jianhua Zhang, Feiyun Xu, Weifeng Xu
White lupin (Lupinus albus L.) produces cluster roots to acquire more phosphorus under phosphorus deficiency. Bacillus amyloliquefaciens SQR9 contributes to plant growth, but whether and how it promotes cluster root formation in white lupin remain unclear. Here, we investigated the roles of SQR9 in cluster root formation under low-phosphorus conditions using a microbial mutant and virus-induced gene
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The cytokinin efflux transporter ABCC4 participates in Arabidopsis root system development Plant Physiol. (IF 6.5) Pub Date : 2024-12-24 Takuya Uragami, Takatoshi Kiba, Mikiko Kojima, Yumiko Takebayashi, Yuzuru Tozawa, Yuki Hayashi, Toshinori Kinoshita, Hitoshi Sakakibara
The directional and sequential flow of cytokinin in plants is organized by a complex network of transporters. Genes involved in several aspects of cytokinin transport have been characterized; however, much of the elaborate system remains elusive. In this study, we used a transient expression system in tobacco (Nicotiana benthamiana) leaves to screen Arabidopsis (Arabidopsis thaliana) transporter genes
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Superoxide anions induce tension wood formation by promoting cambium cell activity Plant Physiol. (IF 6.5) Pub Date : 2024-12-23 Li-Chao Huang, Yu-Yu Li, Jian-Xin Lai, Yi An, Xue-Qin Song, Shu-Tang Zhao, Jin Zhang, Meng-Zhu Lu
Tension wood (TW), characterized by increased cambium cell proliferation and few vessels, is a classical model for the mechanical analysis of wood formation. In this study, we found higher superoxide anion (O2.−) levels in the cambium zone of poplar (Populus alba × P. glandulosa clone “84K”) TW than in that of opposite wood during gravistimulation. Treatment with an O2.− activator (methyl viologen)
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Generating fragrant oilseed rape using CRISPR/Cas9-mediated gene editing Plant Physiol. (IF 6.5) Pub Date : 2024-12-23 Jian Wu, Jingyi Xu, Ancheng He, Sichao Ren, Yi Ye, Wenjing Lei, Yu Liu, Xia Hua, Chunjie Wei, Li Lin, Hui Zhang, Youping Wang
Knocking out 2 functionally redundant aldehyde dehydrogenase-encoding genes via gene editing leads to 2-acetyl-1-pyrroline accumulation in oilseed rape, which can be used to develop fragrant lines.
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Embracing native diversity to enhance the maximum quantum efficiency of photosystem II in maize Plant Physiol. (IF 6.5) Pub Date : 2024-12-23 Sebastian Urzinger, Viktoriya Avramova, Monika Frey, Claude Urbany, Daniela Scheuermann, Thomas Presterl, Stefan Reuscher, Karin Ernst, Manfred Mayer, Caroline Marcon, Frank Hochholdinger, Sarah Brajkovic, Bernardo Ordas, Peter Westhoff, Milena Ouzunova, Chris-Carolin Schön
The sustainability of maize cultivation would benefit tremendously from early sowing, but is hampered by low temperatures during early development in temperate climates. We show that allelic variation within the gene encoding subunit M of the NADH-dehydrogenase-like (NDH) complex (ndhm1) in a European maize landrace affects several quantitative traits that are relevant during early development in cold
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Genetic variation in Arabidopsis thaliana reveals the existence of natural heat resilience factors for meiosis Plant Physiol. (IF 6.5) Pub Date : 2024-12-23 Jiayi (嘉怡) Zhao (赵), Huiqi Fu, Zhengze Wang, Min Zhang, Yaoqiong Liang, Xueying Cui, Wenjing Pan, Ziming Ren, Zhihua Wu, Yujie Zhang, Xin Gui, Li Huo, Xiaoning Lei, Chong Wang, Arp Schnittger, Wojciech P Pawlowski, Bing Liu
Heat interferes with multiple meiotic processes, leading to genome instability and sterility in flowering plants, including many crops. Despite its importance for food security, the mechanisms underlying heat tolerance of meiosis are poorly understood. In this study, we analyzed different meiotic processes in the Arabidopsis (Arabidopsis thaliana) accessions Col and Ler, their F1 hybrids, and the F2
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The bHLH transcription factor gene EGL3 accounts for the natural diversity in Arabidopsis fruit trichome pattern and morphology Plant Physiol. (IF 6.5) Pub Date : 2024-12-22 Belén Méndez-Vigo, Noelia Arteaga, Alba Murillo-Sánchez, Sonia Alba, Carlos Alonso-Blanco
The number and distribution of trichomes, i.e., the trichome pattern, in different plant organs shows a conspicuous inter- and intraspecific diversity across Angiosperms that is presumably involved in adaptation to numerous environmental factors. The genetic and molecular mechanisms accounting for the evolution of trichome patterns have just begun to be elucidated. In this study, we aimed to identify