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MutL homolog 1 participates in interference-sensitive meiotic crossover formation in soybean Plant Physiol. (IF 7.4) Pub Date : 2024-03-16 Tao Wu, Suxin Yang, Junling Fang, Yongheng Ye, Yaohua Zhang, Jinshan Gao, Jiantian Leng, Zhirui Zhang, Kuanqiang Tang, Javaid Akhter Bhat, Xianzhong Feng
MutL homolog 1 (MLH1), a member of the MutL-homolog family, is required for normal recombination in most organisms. However, its role in soybean (Glycine max) remains unclear to date. Here, we characterized the Glycine max female and male sterility 1 (Gmfms1) mutation that reduces pollen grain viability and increases embryo sac abortion in soybean. Map-based cloning revealed that the causal gene of
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JASMONATE ZIM-DOMAIN PROTEIN 3 regulates photo- and thermo-morphogenesis through inhibiting PIF4 in Arabidopsis Plant Physiol. (IF 7.4) Pub Date : 2024-03-15 Junling Huai, Nan Gao, Yuanyuan Yao, Yanxin Du, Qiang Guo, Rongcheng Lin
Light and temperature are two major environmental factors that affect growth and development of plants during their life cycle. Plants have evolved complex mechanisms to adapt to varying external environments. Here, we show that JASMONATE ZIM-domain protein 3 (JAZ3), a jasmonic acid signaling component, acts as a factor to integrate light and temperature in regulating seedling morphogenesis. JAZ3 overexpression
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TCP transcription factor StAST1 represses potato tuberization by regulating tuberigen complex activity Plant Physiol. (IF 7.4) Pub Date : 2024-03-15 Xiaomeng Sun, Enshuang Wang, Liu Yu, Shengxuan Liu, Tiantian Liu, Jun Qin, Peng Jiang, Shuangshuang He, Xingkui Cai, Shenglin Jing, Botao Song
Potato (Solanum tuberosum L.) is cultivated worldwide for its underground tubers, which provide an important part of human nutrition and serve as a model system for below-ground storage organ formation. Similar to flowering, stolon-expressed FLOWERING LOCUS T-like (FT-like) protein SELF-PRUNING 6A (StSP6A) plays an instrumental role in tuberization by binding to the bZIP transcription factors StABI5-like
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Deciphering Recursive Polyploidization in Lamiales and Reconstructing Their Chromosome Evolutionary Trajectories Plant Physiol. (IF 7.4) Pub Date : 2024-03-14 Jiangli Wang, Bowen Song, Minran Yang, Fubo Hu, Huilong Qi, Huizhe Zhang, Yuelong Jia, Yingjie Li, Zhenyi Wang, Xiyin Wang
Lamiales is an order of core eudicots with abundant diversity, and many Lamiales plants have important medicinal and ornamental values. Here, we comparatively reanalyzed 11 Lamiales species with well-assembled genome sequences and found evidence that Lamiales plants, in addition to a hexaploidization or whole-genome triplication (WGT) shared by core eudicots, experienced further polyploidization events
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Low-CO2-inducible bestrophins outside the pyrenoid sustain high photosynthetic efficacy in diatoms Plant Physiol. (IF 7.4) Pub Date : 2024-03-13 Minori Nigishi, Ginga Shimakawa, Kansei Yamagishi, Ryosuke Amano, Shun Ito, Yoshinori Tsuji, Chikako Nagasato, Yusuke Matsuda
Anion transporters sustain a variety of physiological states in cells. Bestrophins belong to a Cl− and/or HCO3− transporter family conserved in bacteria, animals, algae, and plants. Recently, putative bestrophins were found in the green alga Chlamydomonas reinhardtii, where they are up-regulated under low CO2 conditions and play an essential role in the CO2-concentrating mechanism (CCM). The putative
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A nuclease-dead Cas9-derived tool represses target gene expression Plant Physiol. (IF 7.4) Pub Date : 2024-03-13 Bowen Wang, Xiaolin Liu, Zhenxiang Li, Kang Zeng, Jiangyi Guo, Tongxu Xin, Zhen Zhang, Jian-Feng Li, Xueyong Yang
Manipulation of gene expression is central to understanding gene function, engineering cell behavior, and altering biological traits according to production demands. Nuclease-dead Cas9 (dCas9), a variant of active Cas9, offers a versatile platform for the precise control of genome function without DNA cleavage. Notably, however, an effective and universal dCas9-based transcriptional repression system
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Subtilisin-like proteases from Fusarium graminearum induce plant cell death and contribute to virulence Plant Physiol. (IF 7.4) Pub Date : 2024-03-13 Jiang Xiong, Mingyu Luo, Yunshen Chen, Qianyong Hu, Ying Fang, Tongjun Sun, Guanjing Hu, Cui-Jun Zhang
Fusarium head blight (FHB), caused by Fusarium graminearum, causes huge annual economic losses in cereal production. To successfully colonize host plants, pathogens secrete hundreds of effectors that interfere with plant immunity and facilitate infection. However, the roles of most secreted effectors of F. graminearum in pathogenesis remain unclear. We analyzed the secreted proteins of F. graminearum
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A nanopore-based cucumber genome assembly reveals structural variations at two QTLs controlling hypocotyl elongation Plant Physiol. (IF 7.4) Pub Date : 2024-03-13 Bin Liu, Cheng-Cheng Shen, Shi-Wei Xia, Shan-Shan Song, Li-Hong Su, Yu Li, Qian Hao, Yan-Jun Liu, Dai-Lu Guan, Ning Wang, Wen-Jiao Wang, Xiang Zhao, Huan-Xiu Li, Xi-Xiang Li, Yun-Song Lai
The Xishuangbanna (XIS) cucumber (Cucumis sativus var. xishuangbannanesis) is a semiwild variety that has many distinct agronomic traits. Here, long -reads generated by Nanopore sequencing technology helped assembling a high-quality genome (contig N50 = 8.7 Mb) of landrace XIS49. A total of 10,036 structural/sequence variations (SVs) were identified when comparing with Chinese Long (CL), and known
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Metabolic crosstalk between hydroxylated monoterpenes and salicylic acid in tomato defense response against bacteria Plant Physiol. (IF 7.4) Pub Date : 2024-03-13 Julia Pérez-Pérez, Samuel Minguillón, Elías Kabbas-Piñango, Celia Payá, Laura Campos, Manuel Rodríguez-Concepción, Ana Espinosa-Ruiz, Ismael Rodrigo, José María Bellés, María Pilar López-Gresa, Purificación Lisón
Hydroxylated monoterpenes (HMTPs) are differentially emitted by tomato (Solanum lycopersicum) plants resisting bacterial infection. We have studied the defensive role of these volatiles in the tomato response to bacteria, whose main entrance are through stomatal apertures. Treatments with some HMTPs resulted in stomatal closure and pathogenesis-related protein 1 (PR1) induction. Particularly, α-terpineol
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Genetic engineering of drought and salt tolerant tomato via S-nitrosylated Δ1-pyrroline-5-carboxylate reductase Plant Physiol. (IF 7.4) Pub Date : 2024-03-13 Wei Liu, Jin-Wei Wei, Qing Shan, Minghui Liu, Jinghao Xu, Biao Gong
Drought and soil salinization substantially impact agriculture. While proline's role in enhancing stress tolerance is known, the exact molecular mechanism by which plants process stress signals and control proline synthesis under stress is still not fully understood. In tomato (Solanum lycopersicum L.), drought and salt stress stimulate nitric oxide (NO) production, which boosts proline synthesis by
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Chromosome ends initiate homologous chromosome pairing during rice meiosis Plant Physiol. (IF 7.4) Pub Date : 2024-03-13 Hanli You, Ding Tang, Huixin Liu, Yue Zhou, Yafei Li, Yi Shen, Zhiyun Gong, Hengxiu Yu, Minghong Gu, Jiming Jiang, Tao Zhang, Zhukuan Cheng
During meiotic prophase I, chromosomes undergo large-scale dynamics to allow homologous chromosome pairing, prior to which chromosome ends attach to the inner nuclear envelope and form a chromosomal bouquet. Chromosome pairing is crucial for homologous recombination and accurate chromosome segregation during meiosis. However, the specific mechanism by which homologous chromosomes recognize each other
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Peroxisomal 4-coumaroyl-CoA ligases participate in shikonin production in Lithospermum erythrorhizon Plant Physiol. (IF 7.4) Pub Date : 2024-03-13 Kohei Nakanishi, Hao Li, Takuji Ichino, Kanade Tatsumi, Keishi Osakabe, Bunta Watanabe, Koichiro Shimomura, Kazufumi Yazaki
4-Coumaroyl-CoA ligase (4CL) is a key enzyme in the phenylpropanoid pathway, which is involved in the biosynthesis of various specialized metabolites such as flavonoids, coumarins, lignans, and lignin. Plants have several 4CLs showing divergence in sequence: class I 4CLs involved in lignin metabolism, class II 4CLs associated with flavonoid metabolism, and atypical 4CLs and 4CL-like proteins of unknown
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Transcription factor OsWRKY72 controls rice leaf angle by regulating LAZY1-mediated shoot gravitropism Plant Physiol. (IF 7.4) Pub Date : 2024-03-13 Lei Liu, Lirong Zhao, Yunwei Liu, Yi Zhu, Shidie Chen, Lu Yang, Xia Li, Wanqin Chen, Zhiyu Xu, Peng Xu, Houping Wang, Diqiu Yu
Leaf angle is a major trait of ideal architecture, which is considered to influence rice (Oryza sativa) cultivation and grain yield. Although a few mutants with altered rice leaf inclination angle have been reported, the underlying molecular mechanism remains unclear. In this study, we showed that a WRKY transcription factor gene, OsWRKY72, was highly expressed in the leaf sheath and lamina joint.
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ABSCISIC ACID-INSENSITIVE 5-KIP-RELATED PROTEIN 1-SHOOT MERISTEMLESS modulates reproductive development of Arabidopsis Plant Physiol. (IF 7.4) Pub Date : 2024-03-11 Wan-Ni Wang, Yu-Ting Wei, Sheng-Ting Zhao, Fu-Huan Yu, Jing-wen Wang, Cheng-yue Gu, Xin-Ran Liu, Na Sai, Jin-Lei Zhu, Qi-Meng Wang, Qin-Xin Bao, Xin-Rong Mu, Yu-xin Liu, Gary J Loake, Ji-hong Jiang, Lai-Sheng Meng
Soil (or plant) water deficit accelerates plant reproduction. However, the underpinning molecular mechanisms remain unknown. By modulating cell division/number, ABSCISIC ACID-INSENSITIVE 5 (ABI5), a key bZIP (basic (region) leucine zippers) transcription factor, regulates both seed development and abiotic stress responses. The KRP (KIP-RELATED PROTEIN) cyclin-dependent kinases (CDKs) play an essential
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Glutamine induces lateral root initiation, stress responses, and disease resistance in Arabidopsis Plant Physiol. (IF 7.4) Pub Date : 2024-03-11 Hong-Sheng Liao, Kim-Teng Lee, Yi-Hsin Chung, Soon-Ziet Chen, Yi-Jie Hung, Ming-Hsiun Hsieh
The production of glutamine (Gln) from NO3- and NH4+ requires ATP, reducing power, and carbon skeletons. Plants may redirect these resources to other physiological processes using Gln directly. However, feeding Gln as the sole nitrogen (N) source has complex effects on plants. Under optimal concentrations, Arabidopsis (Arabidopsis thaliana) seedlings grown on Gln have similar primary root lengths,
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ROP GTPases with a geranylgeranylation motif modulate alkaloid biosynthesis in Catharanthus roseus Plant Physiol. (IF 7.4) Pub Date : 2024-03-11 Dikki Pedenla Bomzan, Anuj Sharma, Pamela Lemos Cruz, Ines Carqueijeiro, Léo Bellenger, Avanish Rai, Akshay Kumar Thippesh, Venkatesha S Chinnegowda, Durgesh Parihar, Eric Ducos, Vincent Courdavault, Dinesh A Nagegowda
Rho of Plant (ROP) GTPases function as molecular switches that control signaling processes essential for growth, development, and defense. However, their role in specialized metabolism is poorly understood. Previously, we demonstrated that inhibition of protein geranylgeranyl transferase (PGGT-I) negatively impacts the biosynthesis of monoterpenoid indole alkaloids (MIA) in Madagascar periwinkle (Catharanthus
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MicroRNA1432 regulates rice drought stress tolerance by targeting the CALMODULIN-LIKE2 gene Plant Physiol. (IF 7.4) Pub Date : 2024-03-11 Guangyu Luo, Lin Li, Xiaoyu Yang, Yu Yu, Lei Gao, Beixin Mo, Xuemei Chen, Lin Liu
Due to climate change, drought has become a major threat to rice (Oryza sativa L.) growth and yield worldwide. Understanding the genetic basis of drought tolerance in rice is therefore of great importance. Here, we identified a microRNA, miR1432, which regulates rice drought tolerance by targeting the CALMODULIN-LIKE2 (OsCaML2) gene. Mutation of MIR1432 (Bhardwaj et al., 2014). Moreover, recent studies
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Woolly mutation with Get02 locus overcomes the polygenic nature of trichome-based pest resistance in tomato Plant Physiol. (IF 7.4) Pub Date : 2024-03-11 Eloisa Vendemiatti, Inty O Hernández-De Lira, Roxane Snijders, Tanmayee Torne -Srivastava, Rodrigo Therezan, Gabriela Simioni Prants, Carlos Lopez-Ortiz, Umesh Reddy, Petra Bleeker, Craig A Schenck, Lázaro Eustáquio Pereira Peres, Vagner Augusto Benedito
Type-IV glandular trichomes, which only occur in the juvenile developmental phase of the cultivated tomato (Solanum lycopersicum), produce acylsugars that broadly protect against arthropod herbivory. Previously, we introgressed the capacity to retain type-IV trichomes in the adult phase from the wild tomato, Solanum galapagense, into the cultivated species cv. Micro-Tom (MT). The resulting MT-Get (MicroTom-Galapagos
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ARGONAUTE1-binding Tudor domain proteins function in small interfering RNA production for RNA-directed DNA methylation Plant Physiol. (IF 7.4) Pub Date : 2024-03-06 Takahito Takei, Michio Tsukada, Kentaro Tamura, Ikuko Hara-Nishimura, Yoichiro Fukao, Yukio Kurihara, Minami Matsui, Hidetoshi Saze, Masayuki Tsuzuki, Yuichiro Watanabe, Takahiro Hamada
Transposable elements (TEs) contribute to plant evolution, development, and adaptation to environmental changes, but the regulatory mechanisms are largely unknown. RNA-directed DNA methylation (RdDM) is one TE regulatory mechanism in plants. Here, we identified that novel ARGONAUTE 1 (AGO1)-binding Tudor domain proteins Precocious dissociation of sisters C/E (PDS5C/E) are involved in 24-nt siRNA production
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Ethylene regulates auxin-mediated root gravitropic machinery and controls root angle in cereal crops Plant Physiol. (IF 7.4) Pub Date : 2024-03-06 Xiuzhen Kong, Yali Xiong, Xiaoyun Song, Samuel Wadey, Suhang Yu, Jinliang Rao, Aneesh Lale, Marco Lombardi, Riccardo Fusi, Rahul Bhosale, Guoqiang Huang
Root angle is a critical factor in optimising the acquisition of essential resources from different soil depths. The regulation of root angle relies on the auxin-mediated root gravitropism machinery. While the influence of ethylene on auxin levels is known, its specific role in governing root gravitropism and angle remains uncertain, particularly when Arabidopsis (Arabidopsis thaliana) core ethylene
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CALMODULIN-LIKE16 and PIN-LIKES7a cooperatively regulate rice seedling primary root elongation under chilling Plant Physiol. (IF 7.4) Pub Date : 2024-03-06 Shuang Liu, Yuying Zheng, Liyan Zhao, Mihray Gulam, Aman Ullah, Guosheng Xie
Low-temperature sensitivity at the germination stage is a challenge for direct seeding of rice in Asian countries. How Ca2+ and IAA signaling regulate primary root growth under chilling remains unexplored. Here, we showed that OsCML16 interacted specifically with OsPILS7a to improve primary root elongation of early rice seedlings under chilling. OsCML16, a subgroup 6c member of the OsCML family, interacted
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Glucose-G protein signaling plays a crucial role in tomato resilience to high temperature and elevated CO2 Plant Physiol. (IF 7.4) Pub Date : 2024-03-06 Jiao Wang, Qian Luo, Xiao Liang, Hua Liu, Changqi Wu, Hanmo Fang, Xuanbo Zhang, Shuting Ding, Jingquan Yu, Kai Shi
Global climate change is accompanied by carbon dioxide (CO2) enrichment and high-temperature stress; however, how plants adapt to the combined environments and the underlying mechanisms remain largely unclear. Here, we show that elevated CO2 alleviated plant sensitivity to high-temperature stress, with significantly increased apoplastic glucose (Glc) levels in tomato (Solanum lycopersicum) leaves.
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Transcription factor CsMYB36 regulates fruit neck length via mediating cell expansion in cucumber Plant Physiol. (IF 7.4) Pub Date : 2024-03-06 Chunhua Wang, Hongxin Yao, Chen Wang, Luyao Gao, Xingwen Chai, Kai Fang, Yalin Du, Ning Hao, Jiajian Cao, Tao Wu
The fruit neck is an important agronomic trait of cucumber (Cucumis sativus). However, the underlying genes and regulatory mechanisms involved in fruit neck development are poorly understood. We previously identified a cucumber yellow green peel (ygp) mutant, whose causal gene is MYB DOMAIN PROTEIN 36 (CsMYB36). This study showed that the ygp mutant exhibited a shortened fruit neck and repressed cell
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Stripe rust effector Pst03724 modulates host immunity by inhibiting NAD kinase activation by a calmodulin Plant Physiol. (IF 7.4) Pub Date : 2024-03-05 Yanfeng Wang, Cong Liu, Yuanyang Qin, Yuanyuan Du, Chao Song, Zhensheng Kang, Jia Guo, Jun Guo
Puccinia striiformis f. sp. tritici (Pst) secretes effector proteins that enter plant cells to manipulate host immune processes. In this report we present an important Pst effector, Pst03724, whose mRNA expression level increases during Pst infection of wheat (Triticum aestivum). Silencing of Pst03724 reduced the growth and development of Pst. Pst03724 targeted the wheat calmodulin TaCaM3-2B, a positive
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GBF family member PfGBF3 and NAC family member PfNAC2 regulate rosmarinic acid biosynthesis under high light Plant Physiol. (IF 7.4) Pub Date : 2024-03-05 Guanwen Xie, Xiuzai Zou, Zishan Liang, Ke Zhang, Duan Wu, Honglei Jin, Hongbin Wang, Qi Shen
Rosmarinic acid (RA) is an important medicinal metabolite and a potent food antioxidant. We discovered that exposure to high light intensifies the accumulation of RA in the leaves of perilla (Perilla frutescens (L.) Britt). However, the molecular mechanism underlying RA synthesis in response to high light stress remains poorly understood. To address this knowledge gap, we conducted a comprehensive
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Anthocyanin biosynthesis in goji berry is inactivated by deletion in a bHLH transcription factor LrLAN1b promoter Plant Physiol. (IF 7.4) Pub Date : 2024-03-03 Tingting Li, Jingjin Wang, Zihan Zhang, Yunfang Fan, Huan Qin, Yue Yin, Guoli Dai, Youlong Cao, Lin Tang
Black goji berry (Lycium ruthenicum Murray) contains a rich source of health-promoting anthocyanins which are used in herbal medicine and nutraceutical foods in China. A natural variant producing white berries allowed us to identify two key genes involved in the regulation of anthocyanin biosynthesis in goji berries: one encoding a MYB transcription factor (LrAN2-like) and one encoding a basic helix-loop-helix
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Triacylglycerol stability limits futile cycles and inhibition of carbon capture in oil-accumulating leaves Plant Physiol. (IF 7.4) Pub Date : 2024-03-03 Brandon S Johnson, Doug K Allen, Philip D Bates
Engineering plant vegetative tissue to accumulate triacylglycerols (TAG, e.g., oil) can increase the amount of oil harvested per acre to levels that exceed current oilseed crops. Engineered tobacco (Nicotiana tabacum) lines that accumulate 15% to 30% oil of leaf dry weight resulted in starkly different metabolic phenotypes. In-depth analysis of the leaf lipid accumulation and 14CO2 tracking describe
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Maize requires Arogenate Dehydratase 2 for resistance to Ustilago maydis and plant development Plant Physiol. (IF 7.4) Pub Date : 2024-03-03 Ru Chang Ren, Ling Guang Kong, Guang Ming Zheng, Ya Jie Zhao, Xin Jiang, Jia Wen Wu, Cuimei Liu, Jinfang Chu, Xin Hua Ding, Xian Sheng Zhang, Guan Feng Wang, Xiang Yu Zhao
Maize (Zea mays) smut is a common biotrophic fungal disease caused by Ustilago maydis and leads to low maize yield. Maize resistance to U. maydis is a quantitative trait. However, the molecular mechanism underlying the resistance of maize to U. maydis is poorly understood. Here, we reported that a maize mutant caused by a single gene mutation exhibited defects in both fungal resistance and plant development
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Phosphorylation of sugar transporter TST2 by protein kinase CPK27 enhances drought tolerance in tomato Plant Physiol. (IF 7.4) Pub Date : 2024-03-03 Changan Zhu, Beiyu Jing, Teng Lin, Xinyan Li, Min Zhang, Yanhong Zhou, Jingquan Yu, Zhangjian Hu
Drought is a major environmental stress threatening plant growth and productivity. Calcium-dependent protein kinases (CPKs) are plant-specific Ca2+ sensors with multifaceted roles in signaling drought responses. Nonetheless, the mechanisms underpinning how CPKs transmit downstream drought signaling remain unresolved. Through genetic investigations, our study unveiled that knocking out CPK27 reduces
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Acyl carrier protein OsMTACP2 confers rice cold tolerance at the booting stage Plant Physiol. (IF 7.4) Pub Date : 2024-03-03 Xiu-Li Hou, Xiangyan Han, Ying Meng, Lizhi Wang, Wenqi Zhang, Chao Yang, Hui Li, Shanjie Tang, Zhenhua Guo, Chunyan Liu, Yongmei Qin, Shaohua Zhang, Guanghou Shui, Xiaofeng Cao, Xianwei Song
Low temperatures occurring at the booting stage in rice (Oryza sativa L.) often result in yield loss by impeding male reproductive development. However, the underlying mechanisms by which rice responds to cold at this stage remain largely unknown. Here, we identified MITOCHONDRIAL ACYL CARRIER PROTEIN 2 (OsMTACP2), the encoded protein of which mediates lipid metabolism involved in the cold response
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The Ginkgo biloba microRNA160–ERF4 module participates in terpene trilactone biosynthesis Plant Physiol. (IF 7.4) Pub Date : 2024-03-03 Jiarui Zheng, Xiao He, Xian Zhou, Xiaomeng Liu, Yuwei Yi, Dongxue Su, Weiwei Zhang, Yongling Liao, Jiabao Ye, Feng Xu
Terpene trilactones (TTLs) are important secondary metabolites in ginkgo (Ginkgo biloba); however, their biosynthesis gene regulatory network remains unclear. Here, we isolated a G. biloba ethylene response factors 4 (GbERF4) involved in TTL synthesis. Overexpression of GbERF4 in tobacco (Nicotiana tabacum) significantly increased terpenoid content and upregulated the expression of key enzyme genes
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Transcription factor NtNAC56 regulates jasmonic acid-induced leaf senescence in tobacco Plant Physiol. (IF 7.4) Pub Date : 2024-03-01 Wei Chang, Huina Zhao, Hongqiao Chen, Guixiang Jiao, Jing Yu, Bing Wang, Haiqian Xia, Boyu Meng, Xiaodong Li, Mengna Yu, Shengting Li, Mingchao Qian, Yonghai Fan, Kai Zhang, Bo Lei, Kun Lu
Leaf senescence is a vital aspect of plant physiology and stress responses and is induced by endogenous factors and environmental cues.. The plant-specific NAC (NAM, ATAF1/2, CUC2) transcription factor family influences growth, development, and stress responses in Arabidopsis (Arabidopsis thaliana) and other species. However, the roles of NACs in tobacco (Nicotiana tabacum) leaf senescence are still
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Transcription factor AHL17 fine-tunes reactive oxygen species accumulation and cassava disease resistance Plant Physiol. (IF 7.4) Pub Date : 2024-02-28 Ruoyu Bai, Mengmeng Zhou, Yu Zheng, Haitao Shi
Zinc finger protein 67 interacts with a transcription factor to fine-tune reactive oxygen species accumulation and immune responses during pathogen infection in cassava.
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High-quality genome assembly enables prediction of allele-specific gene expression in hybrid poplar Plant Physiol. (IF 7.4) Pub Date : 2024-02-27 Tian-Le Shi, Kai-Hua Jia, Yu-Tao Bao, Shuai Nie, Xue-Chan Tian, Xue-Mei Yan, Zhao-Yang Chen, Zhi-Chao Li, Shi-Wei Zhao, Hai-Yao Ma, Ye Zhao, Xiang Li, Ren-Gang Zhang, Jing Guo, Wei Zhao, Yousry Aly El-Kassaby, Niels Müller, Yves Van de Peer, Xiao-Ru Wang, Nathaniel Robert Street, Ilga Porth, Xinmin An, Jian-Feng Mao
Poplar (Populus) is a well-established model system for tree genomics and molecular breeding, and hybrid poplar is widely used in forest plantations. However, distinguishing its diploid homologous chromosomes is difficult, complicating advanced functional studies on specific alleles. In this study, we applied a trio-binning design and PacBio High-Fidelity long-read sequencing to obtain haplotype-phased
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Chloroplast phosphatases LPPγ and LPPε1 facilitate conversion of extraplastidic phospholipids to galactolipids Plant Physiol. (IF 7.4) Pub Date : 2024-02-24 Ron Cook, John E Froehlich, Yang Yang, Ilayda Korkmaz, David M Kramer, Christoph Benning
Galactolipids comprise the majority of chloroplast membranes in plants, and their biosynthesis requires dephosphorylation of phosphatidic acid at the chloroplast envelope membranes. In Arabidopsis (Arabidopsis thaliana), the lipid phosphate phosphatases LPPγ, LPPε1, and LPPε2 have been previously implicated in chloroplast lipid assembly, with LPPγ being essential, as null mutants were reported to exhibit
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Plant iron status regulates ammonium-use efficiency through protein N-glycosylation Plant Physiol. (IF 7.4) Pub Date : 2024-02-24 Guangjie Li, Lin Zhang, Jinlin Wu, Zhaoyue Wang, Meng Wang, Herbert J Kronzucker, Weiming Shi
Improving nitrogen-use efficiency (NUE) is an important path toward enhancing crop yield and alleviating the environmental impacts of fertilizer use. Ammonium (NH4+) is the energetically preferred inorganic N source for plants. The interaction of NH4+ with other nutrients is a chief determinant of ammonium-use efficiency (AUE) and of the tipping point toward ammonium toxicity, but these interactions
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A low-cost open-source imaging platform reveals spatiotemporal insight into leaf elongation and movement Plant Physiol. (IF 7.4) Pub Date : 2024-02-24 Lisa Oskam, Basten L Snoek, Chrysoula K Pantazopoulou, Hans van Veen, Sanne E A Matton, Rens Dijkhuizen, Ronald Pierik
Plant organs move throughout the diurnal cycle, changing leaf and petiole positions to balance light capture, leaf temperature, and water loss under dynamic environmental conditions. Upward movement of the petiole, called hyponasty, is one of several traits of the shade avoidance syndrome (SAS). SAS traits are elicited upon perception of vegetation shade signals such as far-red light (FR) and improve
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Single-cell transcriptome atlas reveals somatic cell embryogenic differentiation features during regeneration Plant Physiol. (IF 7.4) Pub Date : 2024-02-24 Huihui Guo, Li Zhang, Haixia Guo, Xiwang Cui, Yupeng Fan, Tongtong Li, Xiushan Qi, Tongdi Yan, Aiyun Chen, Fengjuan Shi, Fanchang Zeng
Understanding somatic cell totipotency remains a challenge facing scientific inquiry today. Plants display remarkable cell totipotency expression, illustrated by single-cell differentiation during somatic embryogenesis (SE) for plant regeneration. Determining cell identity and exploring gene regulation in such complex heterogeneous somatic cell differentiation have been major challenges. Here, we performed
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TRANSPARENT TESTA GLABRA2 defines trichome cell shape by modulating actin cytoskeleton in Arabidopsis thaliana Plant Physiol. (IF 7.4) Pub Date : 2024-02-23 Lu Liu, Yali Wang, Weihua Cao, Lan Yang, Chi Zhang, Lanxin Yuan, Dan Wang, Wenjia Wang, Hongchang Zhang, John Schiefelbein, Fei Yu, Lijun An
The Arabidopsis (Arabidopsis thaliana) TRANSPARENT TESTA GLABRA2 (TTG2) gene encodes a WRKY transcription factor that regulates a range of development events like trichome, seed coat, and atrichoblast formation. Loss-of-function of TTG2 was previously shown to reduce or eliminate trichome specification and branching. Here, we report the identification of an allele of TTG2, ttg2-6. In contrast to the
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Transcription factor CrWRKY42 coregulates chlorophyll degradation and carotenoid biosynthesis in citrus Plant Physiol. (IF 7.4) Pub Date : 2024-02-23 Hongyan Chen, Huiyu Ji, Wenkai Huang, Zhehui Zhang, Kaijie Zhu, Shiping Zhu, Lijun Chai, Junli Ye, Xiuxin Deng
Chlorophyll degradation and carotenoid biosynthesis, which occur almost simultaneously during fruit ripening, are essential for the coloration and nutritional value of fruits. However, the synergistic regulation of these two processes at the transcriptional level remains largely unknown. Here, we identified a WRKY transcription factor, CrWRKY42, from the transcriptome data of the yellowish bud mutant
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2-Cys peroxiredoxins contribute to thylakoid lipid unsaturation by affecting ω-3 Fatty Acid Desaturase 8 Plant Physiol. (IF 7.4) Pub Date : 2024-02-22 María Luisa Hernández, Julia Jiménez-López, Francisco Javier Cejudo, Juan Manuel Pérez-Ruiz
Fatty acid unsaturation levels affect chloroplast function and plant acclimation to environmental cues. However, the regulatory mechanism(s) controlling fatty acid unsaturation in thylakoid lipids is poorly understood. Here, we have investigated the connection between chloroplast redox homeostasis and lipid metabolism by focusing on 2-Cys peroxiredoxins (Prxs), which play a central role balancing the
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Catalytically impaired chalcone isomerase retains flavonoid biosynthetic capacity Plant Physiol. (IF 7.4) Pub Date : 2024-02-22 Koichi Sugimoto, Niloufer Irani, Erich Grotewold, Gregg A Howe
Catalytically impaired chalcone isomerases having no appreciable activity in vitro retain the ability to robustly promote flavonoid biosynthesis in planta.
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CRISPR/Cas9-mediated mutagenesis of rice NAC transcription factor genes results in altered innate immunity Plant Physiol. (IF 7.4) Pub Date : 2024-02-22 Seungmin Son, Giha Song, Suhyeon Nam, Gunhee Lee, Jeonghui Im, Kyong Sil Lee, Yeo Jin Park, Eun-Jung Suh, Sang Ryeol Park
Mutating transcription factor genes involved in growth, development, and stress response in rice enhances disease resistance to microbial pathogens without suffering a yield penalty.
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Thioredoxin A regulates protein synthesis to maintain carbon and nitrogen partitioning in cyanobacteria Plant Physiol. (IF 7.4) Pub Date : 2024-02-22 Manuel J Mallén-Ponce, Francisco Javier Florencio, María José Huertas
Thioredoxins play an essential role in regulating enzyme activity in response to environmental changes, especially in photosynthetic organisms. They are crucial for metabolic regulation in cyanobacteria, but the key redox-regulated central processes remain to be determined. Physiological, metabolic, and transcriptomic characterization of a conditional mutant of the essential Synechocystis sp. PCC 6803
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All Members of the Arabidopsis DGAT and PDAT Acyltransferase Families Operate During High and Low Temperatures Plant Physiol. (IF 7.4) Pub Date : 2024-02-22 Zachery D Shomo, Samira Mahboub, Hathaichanok Vanviratikul, Mason McCormick, Tatpong Tulyananda, Rebecca L Roston, Jaruswan Warakanont
The accumulation of triacylglycerol (TAG) in vegetative tissues is necessary to adapt to changing temperatures. It has been hypothesized that TAG accumulation is required as a storage location for maladaptive membrane lipids. The TAG acyltransferase family has five members (DIACYLGLYCEROL ACYLTRANSFERSE1/2/3 and PHOSPHOLIPID:DIACYLGLYCEROL ACYLTRANSFERASE1/2), and their individual roles during temperature
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Increased α-ketoglutarate links the C3-C4 intermediate state to C4 photosynthesis in the genus Flaveria Plant Physiol. (IF 7.4) Pub Date : 2024-02-20 Qiming Tang, Yuhui Huang, Xiaoxiang Ni, Ming-Ju Amy Lyu, Genyun Chen, Rowan Sage, Xin-Guang Zhu
As a complex trait, C4 photosynthesis has multiple independent origins in evolution. Phylogenetic evidence and theoretical analysis suggest that C2 photosynthesis, which is driven by glycine decarboxylation in the bundle sheath cell, may function as a bridge from C3 towards C4 photosynthesis. However, the exact molecular mechanism underlying the transition between C2 photosynthesis towards C4 photosynthesis
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Double blocking of carbon metabolism causes a large increase of Calvin-Benson cycle compounds in cyanobacteria Plant Physiol. (IF 7.4) Pub Date : 2024-02-20 María Teresa Domínguez-Lobo, Miguel Roldán, Alba María Gutiérrez-Diánez, Francisco Javier Florencio, MaríaIsabel Muro-Pastor
Carbon-flow-regulator A (CfrA) adapts carbon flux to nitrogen conditions in non-diazotrophic cyanobacteria. Under nitrogen deficiency, CfrA leads to the storage of excess carbon, which cannot combine with nitrogen, mainly as glycogen. cfrA overexpression from the arsenite-inducible, nitrogen-independent ParsB promoter allows analysis of the metabolic effects of CfrA accumulation. Considering that the
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Far-red light-enhanced apical dominance stimulates flower and fruit abortion in sweet pepper Plant Physiol. (IF 7.4) Pub Date : 2024-02-17 Sijia Chen, Leo F M Marcelis, Remko Offringa, Wouter Kohlen, Ep Heuvelink
Far-red radiation affects many plant processes, including reproductive organ abortion. Our research aimed to determine the role of apical dominance in far-red light-induced flower and fruit abortion in sweet pepper (Capsicum annuum L.). We conducted several climate room experiments where plants were grown under white or red-rich LED light, with or without additional far-red light. Additional far-red
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RNA-directed DNA methylation mutants reduce histone at the paramutated maize booster1 enhancer Plant Physiol. (IF 7.4) Pub Date : 2024-02-17 Iris Hövel, Rechien Bader, Marieke Louwers, Max Haring, Kevin Peek, Jonathan I Gent, Maike Stam
Paramutation is the transfer of mitotically and meiotically heritable silencing information between two alleles. With paramutation at the maize (Zea mays) booster1 (b1) locus, the low-expressed B’ epiallele heritably changes the high-expressed B-I epiallele into B’ with 100% frequency. This requires specific tandem repeats and multiple components of the RNA-directed DNA methylation (RdDM) pathway,
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Overcoming constraints to measuring O2 diffusivity and consumption of intact roots Plant Physiol. (IF 7.4) Pub Date : 2024-02-17 Juan de la Cruz Jiménez, William Armstrong, Timothy D Colmer, Ole Pedersen
A method using O2 microsensors enables detailed quantification of respiratory O2 consumption and diffusive resistance to O2 of individual root cell layers.
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Transcription factors VviWRKY10 and VviWRKY30 co-regulate powdery mildew resistance in grapevine Plant Physiol. (IF 7.4) Pub Date : 2024-02-17 Min Zhou, Hongyan Wang, Xuena Yu, Kaicheng Cui, Yang Hu, Shunyuan Xiao, Ying-Qiang Wen
Grapevine (Vitis vinifera) is an economically important fruit crop worldwide. The widely cultivated grapevine is susceptible to powdery mildew caused by Erysiphe necator. In this study, we used CRISPR-Cas9 to simultaneously knock out VviWRKY10 and VviWRKY30 encoding two transcription factors reported to be implicated in defense regulation. We generated 53 wrky10 single mutant transgenic plants and
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Population epigenetics: DNA methylation in the plant omics era Plant Physiol. (IF 7.4) Pub Date : 2024-02-17 Jie Liu, Xuehua Zhong
DNA methylation plays important roles in many biological processes. The mechanisms underlying the establishment and maintenance of DNA methylation are well understood thanks to decades of research using DNA methylation mutants, primarily in Arabidopsis (Arabidopsis thaliana) accession Columbia-0 (Col-0). Recent genome-wide association studies (GWAS) using the methylomes of natural accessions have uncovered
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Defying Gravity: WEEP promotes negative gravitropism in peach trees by establishing asymmetric auxin gradients Plant Physiol. (IF 7.4) Pub Date : 2024-02-17 Andrea R Kohler, Andrew Scheil, Joseph L Hill, Jeffrey R Allen, Jameel M Al-Haddad, Charity Z Goeckeritz, Lucia C Strader, Frank W Telewski, Courtney A Hollender
Trees with weeping shoot architectures are valued for their beauty and are a resource for understanding how plants regulate posture control. The peach (Prunus persica) weeping phenotype, which has elliptical downward arching branches, is caused by a homozygous mutation in the WEEP gene. Little is known about the function of WEEP despite its high conservation throughout Plantae. Here, we present the
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Heat stress at the bicellular stage inhibits sperm cell development and transport into pollen tubes Plant Physiol. (IF 7.4) Pub Date : 2024-02-17 Xingli Li, Astrid Bruckmann, Thomas Dresselhaus, Kevin Begcy
For successful double fertilization in flowering plants (angiosperms), pollen tubes deliver two non-motile sperm cells towards female gametes (egg and central cell, respectively). Heatwaves, especially during the reproduction period, threaten male gametophyte (pollen) development, resulting in severe yield losses. Using maize (Zea mays) as a crop and grass model system, we found strong seed set reduction
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Redirecting Barley Breeding for Grass Production through Genome Editing of Photoperiod-H1 Plant Physiol. (IF 7.4) Pub Date : 2024-02-17 Daisuke Tezuka, Huikyong Cho, Hitomi Onodera, Qianyan Linghu, Takeshi Chijimatsu, Masahiro Hata, Ryozo Imai
Genome editing enables precise modification to harness an elite grain-producing barley (Hordeum vulgare L.) cultivar for grass production.
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FLOWERING LOCUS T1 and TERMINAL FLOWER1 regulatory networks mediate flowering initiation in apple Plant Physiol. (IF 7.4) Pub Date : 2024-02-17 Xiya Zuo, Shixiang Wang, Xiuxiu Liu, Ting Tang, Youmei Li, Lu Tong, Kamran Shah, Juanjuan Ma, Na An, Caiping Zhao, Libo Xing, Dong Zhang
Flower bud formation is a critical process that directly determines yield and fruit quality in fruit crops. Floral induction is modulated by the balance between two flowering-related proteins, FLOWERING LOCUS T (FT) and TERMINAL FLOWER 1 (TFL1); however, the mechanisms underlying the establishment and maintenance of this dynamic balance remain largely elusive. Here we showed that in apple (Malus ×
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Abscisic acid root-to-shoot translocation by transporter AtABCG25 mediates stomatal movements in Arabidopsis Plant Physiol. (IF 7.4) Pub Date : 2024-02-12 Qin Yang, Xiaojuan Deng, Ting Liu, Jiayun Qian, Penghong Zhang, Engao Zhu, Jingqi Wang, Xiaoxian Zhu, Guzel Kudoyarova, Jiangzhe Zhao, Kewei Zhang
The phytohormone abscisic acid (ABA) plays a central role in regulating stomatal movements under drought conditions. The root-derived peptide CLAVATA3/EMBRYO SURROUNDING REGION-RELATED 25 (CLE25) moves from the root to shoot for activating ABA biosynthesis under drought conditions. However, the root-to-shoot translocation of root-derived ABA and its regulation of stomatal movements in the shoot remain
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Poaceae plants transfer cyclobutane pyrimidine dimer photolyase to chloroplasts for ultraviolet-B resistance Plant Physiol. (IF 7.4) Pub Date : 2024-02-12 Momo Otake, Mika Teranishi, Chiharu Komatsu, Mamoru Hara, Kaoru Okamoto Yoshiyama, Jun Hidema
Photoreactivation enzyme that repairs cyclobutane pyrimidine dimer (CPD) induced by ultraviolet-B radiation, commonly called CPD photolyase (PHR), is essential for plants living under sunlight. Rice (Oryza sativa) PHR (OsPHR) is a unique triple-targeting protein. The signal sequences required for its translocation to the nucleus or mitochondria are located in the C-terminal region but have yet to be
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AGAMOUS-LIKE24 controls pistil number in Japanese apricot by targeting the KNOTTED1-LIKE gene KNAT2/6-a Plant Physiol. (IF 7.4) Pub Date : 2024-02-12 Yang Bai, Pengyu Zhou, Zhaojun Ni, Shahid Iqbal, Kenneth Omondi Ouma, Xiao Huang, Feng Gao, Chengdong Ma, Ting Shi, Zhihong Gao
The formation of multi-pistil flowers reduces the yield and quality in Japanese apricot (Prunus mume). However, the molecular mechanism underlying the formation of multi-pistil flowers remains unknown. In the current study, overexpression of PmKNAT2/6-a, a class I KNOTTED1-like homeobox (KNOX) member, in Arabidopsis (Arabidopsis thaliana) resulted in a multi-pistil phenotype. Analysis of the upstream
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Brassinosteroid regulates stomatal development in etiolated cotyledons via transcription factors BZR1 and BES1 Plant Physiol. (IF 7.4) Pub Date : 2024-02-12 Shuo Li, Jin Yan, Lian-Ge Chen, Guanghua Meng, Yuling Zhou, Chun-Ming Wang, Lei Jiang, Juan Luo, Yueming Jiang, Qian-Feng Li, Wenqiang Tang, Jun-Xian He
Brassinosteroids (BRs) are phytohormones that regulate stomatal development BRASSINAZOLE RESISTANT 1. In this study, we report that BR represses stomatal development in etiolated Arabidopsis (Arabidopsis thaliana) cotyledons via transcription factors BRASSINAZOLE RESISTANT 1 (BZR1) and bri1-EMS SUPPRESSOR1 (BES1), which directly target MITOGEN-ACTIVATED PROTEIN KINASE KINASE 9 (MKK9) and FAMA, two