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  • Matrix Redox Physiology Governs the Regulation of Plant Mitochondrial Metabolism through Post-Translational Protein Modifications
    Plant Cell (IF 8.631) Pub Date : 2020-01-24
    Ian Max Møller, Abir U Igamberdiev, Natalia V. Bykova, Iris Finkemeier, Allan G. Rasmusson, Markus Schwarzländer

    ABSTRACT Mitochondria function as hubs of plant metabolism. Oxidative phosphorylation produces ATP, but it is also a central high-capacity electron sink required by many metabolic pathways that need to be flexibly coordinated and integrated. We here review redox-associated post-translational protein modifications (PTMs) that implement a very significant proportion of mitochondrial metabolic regulation. We conclude that: (i) The major redox couples in the mitochondrial matrix - NAD, NADP, thioredoxin, glutathione and ascorbate - are in kinetic steady-state rather than thermodynamic equilibrium. (ii) Targeted proteomics have produced long lists of proteins potentially regulated by Cys oxidation/Trx, Met-SO formation, phosphorylation, or Lys acetylation. At this point, we only understand the functional importance of relatively few of these PTMs and some of the site modifications may represent 'molecular noise' caused by spurious reactions. (iii) Different PTMs on the same protein or on different proteins in the same metabolic pathway can interact to fine-tune the regulation. (iv) PTMs take part in repair of stress-induced damage, e.g. by reduction of Met and Cys oxidation products, but also in adjusting metabolic functions in response to environmental variation, like changes in light irradiance or oxygen availability. (v) Together, the PTMs form a multidimensional regulatory system that provide essential speed and flexibility to mitochondrial coordination, beyond what can be regulated by changes in nuclear gene expression alone.

    更新日期:2020-01-26
  • GPA5 Encodes a Rab5a Effector Required for Post-Golgi Trafficking of Rice Storage Proteins
    Plant Cell (IF 8.631) Pub Date : 2020-01-21
    Yulong Ren, Yihua Wang, Tian Pan, Yunlong Wang, Yongfei Wang, Lu Gan, Zhongyan Wei, Fan Wang, Mingming Wu, Ruonan Jing, Jiachang Wang, Gexing Wan, Xiuhao Bao, Binglei Zhang, Pengcheng Zhang, Yu Zhang, Yi Ji, Cailin Lei, Xin Zhang, Zhijun Cheng, Qibing Lin, Shanshan Zhu, Zhichao Zhao, Jie Wang, Chuanyin Wu, Lijuan Qiu, Haiyang Wang, Jianmin Wan

    Dense vesicles (DVs) are vesicular carriers, unique to plants, that mediate post-Golgi trafficking of storage proteins to protein storage vacuoles (PSVs) in seeds. However, the molecular mechanisms regulating the directional targeting of DVs to PSVs remains elusive. Here, we show that the rice (Oryza sativa) glutelin precursor accumulation5 (gpa5) mutant is defective in directional targeting of DVs to PSVs, resulting in discharge of its cargo proteins into the extracellular space. Molecular cloning revealed that GPA5 encodes a plant-unique phox-homology domain-containing protein homologous to Arabidopsis thaliana ENDOSOMAL RAB EFFECTOR WITH PX-DOMAIN (EREX). We show that GPA5 is a membrane-associated protein capable of forming homodimers and that it is specifically localized to DVs in developing endosperm. Colocalization, biochemical, and genetic evidence demonstrate that GPA5 acts in concert with Rab5a and VPS9a to regulate DV-mediated post-Golgi trafficking to PSVs. Furthermore, we demonstrated that GPA5 physically interacts with a class C core vacuole/endosome tethering (CORVET) complex and a seed plant-specific VAMP727-containing R-soluble NSF attachment protein receptor (SNARE) complex. Collectively, our results suggest that GPA5 functions as a plant-specific effector of Rab5a required for mediating tethering and membrane fusion of DVs with PSVs in rice endosperm.

    更新日期:2020-01-22
  • A Similar Genetic Architecture Underlies the Convergent Evolution of the Selfing Syndrome in Capsella
    Plant Cell (IF 8.631) Pub Date : 2020-01-21
    Natalia Joanna Wozniak, Christian Kappel, Cindy Marona, Lothar Altschmied, Barbara Neuffer, Adrien Sicard

    Whether, and to what extent, phenotypic evolution follows predictable genetic paths, remains an important question in evolutionary biology. Convergent evolution of similar characters provides a unique opportunity to address this question. The transition to selfing and the associated changes in flower morphology are among the most prominent examples of repeated evolution in plants. Yet, to date no studies have directly compared the extent of similarities between convergent adaptations to selfing. In this study, we take advantage of the independent transitions to self-fertilization in the genus Capsella to test the existence of genetic and developmental constraints imposed on flower evolution in the context of the selfing syndrome. While C. rubella and C. orientalis have emerged independently, both have evolved almost identical flower characters. Not only the evolutionary outcome is identical but, in both cases, the same developmental strategies underlie the convergent reduction of flower size. This has been associated with convergent evolution of gene-expression changes. The transcriptomic changes common to both selfing lineages are enriched in genes with low-network connectivity and with organ-specific expression patterns. Comparative genetic mapping also suggests that, at least in the case of petal size evolution, these similarities have a similar genetic basis. Based on these results we hypothesise that the limited availability of low-pleiotropy paths predetermines closely related species to similar evolutionary outcomes.

    更新日期:2020-01-22
  • PROTEIN PHOSPHATASE 95 Regulates Phosphate Homeostasis by Affecting Phosphate Transporter Trafficking in Rice
    Plant Cell (IF 8.631) Pub Date : 2020-01-17
    Zhili Yang, Jian Yang, Yan Wang, Fei Wang, Wenxuan Mao, Qiuju He, Jiming Xu, Wu Zhongchang, Chuanzao Mao

    Phosphate (Pi) uptake in plants depends on plasma membrane (PM)-localized Pi transporters (PTs). OsCK2 phosphorylates PTs and inhibits their trafficking from the endoplasmic reticulum (ER) to the PM in rice (Oryza sativa), but how PTs are dephosphorylated is unknown. We demonstrate that the protein phosphatases type-2C (PP2C) protein phosphatase OsPP95 interacts with OsPT2 and OsPT8 and dephosphorylates OsPT8 at Ser-517. Rice plants overexpressing OsPP95 reduced OsPT8 phosphorylation and promoted OsPT2 and OsPT8 trafficking from the ER to the PM, resulting in Pi accumulation. Under Pi-sufficient conditions, Pi levels were lower in young leaves and higher in old leaves in ospp95 mutants than in those of the wild type, even though the overall shoot Pi levels were the same in the mutant and wild type. In the wild type, OsPP95 accumulated under Pi starvation but was rapidly degraded under Pi-sufficient conditions. We show that OsPHO2 interacts with and induces the degradation of OsPP95. We conclude that OsPP95, a protein phosphatase negatively regulated by OsPHO2, positively regulates Pi homeostasis and remobilization by dephosphorylating PTs and affecting their trafficking to the PM, a reversible process required for adaptation to variable Pi conditions.

    更新日期:2020-01-21
  • A Kinase and a Glycosylase Catabolize Pseudouridine in the Peroxisome to Prevent Toxic Pseudouridine Monophosphate Accumulation
    Plant Cell (IF 8.631) Pub Date : 2020-01-10
    Mingjia Chen, Claus-Peter Witte

    Pseudouridine () is a frequent nucleoside modification occurring in non-coding RNA but also in mRNA. In pseudouridine, C5 of uracil is attached to the ribose via an unusual C-glycosidic bond. The modification is introduced on the RNA by site-specific transglycosylation of uridine mediated by pseudouridine synthases. RNA is subject to constant turnover, releasing free pseudouridine. The metabolic fate of pseudouridine has not yet been investigated in eukaryotes. We show that Arabidopsis thaliana catabolizes pseudouridine in the peroxisome employing (i) a pseudouridine kinase (PUKI) from the PfkB family, which generates 5'-MP, and (ii) a MP glycosylase (PUMY) hydrolyzing MP to uracil and ribose-5-phosphate. Abrogation of pseudouridine catabolism leads to strong pseudouridine accumulation and also higher MP content. Particularly MP is toxic, causing a delay in germination and growth inhibition, but /U ratios in RNA are not affected. The bipartite peroxisomal PUKI and PUMY are conserved in plants and algae, whereas some fungi and most animals, except mammals, possess a PUMY-PUKI fusion protein, probably located in mitochondria. In our model, vacuolar turnover of rRNA produces the bulk of pseudouridine via 3'-MP, which is imported through the cytosol into the peroxisomes for degradation by PUKI and PUMY involving a toxic 5'-MP intermediate.

    更新日期:2020-01-13
  • The Arabidopsis Nodulin Homeobox Factor AtNDX Interacts with AtRING1A/B and Negatively Regulates Abscisic Acid Signaling
    Plant Cell (IF 8.631) Pub Date : 2020-01-09
    Yujuan Zhu, Xiaoying Hu, Ying Duan, Shaofang Li, Yu Wang, Amin Ur Rehman, Junna He, Jing Zhang, Deping Hua, Li Yang, Li Wang, Zhizhong Chen, Chuanyou Li, Baoshan Wang, Chun-Peng Song, Qianwen Sun, Shuhua Yang, Zhizhong Gong

    The phytohormone abscisic acid (ABA) and the Polycomb group (PcG) proteins have key roles in regulating plant growth and development; however, their interplay and underlying mechanisms are not fully understood. Here, we identified an Arabidopsis thaliana nodulin homeobox (AtNDX) protein as a negative regulator in the ABA signaling pathway. AtNDX mutants are hypersensitive to ABA, as measured by inhibition of seed germination and root growth, and the expression of AtNDX is downregulated by ABA. AtNDX interacts with the Polycomb Repressive Complex 1 (PRC1) core components AtRING1A and AtRING1B in vitro and in vivo, and together, they negatively regulate the expression levels of some ABA-responsive genes. We identified ABA-INSENSITIVE (ABI4) as a direct target of AtNDX. AtNDX directly binds the downstream region of ABI4 and deleting this region increases the ABA sensitivity of primary root growth. Furthermore, ABI4 mutations rescue the ABA-hypersensitive phenotypes of ndx mutants and ABI4-overexpressing plants were hypersensitive to ABA in primary root growth. Thus, our work reveals the critical functions of AtNDX and PRC1 in ABA signaling and their regulation of ABI4.

    更新日期:2020-01-11
  • Genome Evolution in Arabideae was Marked by Frequent Centromere Repositioning
    Plant Cell (IF 8.631) Pub Date : 2020-01-09
    Terezie Mandáková, Petra Hloušková, Marcus A Koch, Martin A. Lysak

    Centromere position may change despite conserved chromosomal collinearity. Centromere repositioning and evolutionary new centromeres (ENC) were frequently encountered during vertebrate genome evolution, but only rarely observed in plants. The largest crucifer tribe Arabideae (c. 550 species; Brassicaceae, the mustard family) diversified into several well-defined subclades in the virtual absence of chromosome number variation. BAC-based comparative chromosome painting uncovered a constancy of genome structures among 10 analyzed genomes representing seven Arabideae subclades classified as four genera (Arabis, Aubrieta, Draba and Pseudoturritis). Interestingly, the intra-tribal diversification was marked by the high frequency of ENCs on five out of the eight homeologous chromosomes in the crown-group genera, but not in the most ancestral Pseudoturritis genome. From the 32 documented ENCs, at least 26 originated independently including four ENCs recurrently formed at the same position in not closely related species. While chromosomal localization of ENCs does not reflect the phylogenetic position of the Arabideae subclades, centromere seeding was usually confined to long chromosome arms, transforming acrocentric chromosomes to (sub)metacentric ones. Centromere repositioning is proposed as the key mechanism differentiating overall conserved homeologous chromosomes across the crown-group Arabideae subclades. The evolutionary significance of centromere repositioning is discussed in the context of possible adaptive effects on recombination and epigenetic regulation of gene expression.

    更新日期:2020-01-11
  • Kinesin-13 and Kinesin-8 Function During Cell Growth and Division in the Moss Physcomitrella patens
    Plant Cell (IF 8.631) Pub Date : 2020-01-09
    Shu Yao Leong, Tomoya Edzuka, Gohta Goshima, Moé Yamada

    Kinesin-13 and -8 are well-known microtubule (MT) depolymerases that regulate MT length and chromosome movement in animal mitosis. While much is unknown about plant Kinesin-8, Arabidopsis thaliana and rice (Oryza sativa) Kinesin-13 have been shown to depolymerise MTs in vitro. However, the mitotic function of both kinesins has yet to be determined in plants. Here, we generated complete null mutants of Kinesin-13 and -8 in the moss Physcomitrella patens. Both kinesins were found to be non-essential for viability, but the Kinesin-13 knockout (KO) line had increased mitotic duration and reduced spindle length, whereas the Kinesin-8 KO line did not display obvious mitotic defects. Surprisingly, spindle MT poleward flux, which is mediated by Kinesin-13 in animals, was retained in the absence of Kinesin-13. MT depolymerase activity was not detectable for either kinesin in vitro, while MT catastrophe inducing (Kinesin-13) or MT gliding (Kinesin-8) activity was observed. Interestingly, both KO lines showed waviness in their protonema filaments, which correlated with positional instability of the MT foci in their tip cells. Taken together, the results suggest that plant Kinesin-13 and -8 have diverged in both mitotic function and molecular activity, acquiring roles in regulating MT foci positioning for directed tip growth.

    更新日期:2020-01-11
  • 更新日期:2020-01-11
  • Ubiquitous Ubiquitin: The K63 Ubiquitinome
    Plant Cell (IF 8.631) Pub Date : 2020-01-01
    Emily Breeze
    更新日期:2020-01-11
  • Predicting Adult Complex Traits from Early Development Transcript Data in Maize
    Plant Cell (IF 8.631) Pub Date : 2020-01-01
    Sunil K. Kenchanmane Raju
    更新日期:2020-01-11
  • 更新日期:2020-01-11
  • 更新日期:2020-01-11
  • Celebrating 20 Years of Genetic Discoveries in Legume Nodulation and Symbiotic Nitrogen Fixation
    Plant Cell (IF 8.631) Pub Date : 2020-01-01
    Sonali Roy, Wei Liu, Raja Sekhar Nandety, Ashley Crook, Kirankumar S. Mysore, Catalina I. Pislariu, Julia Frugoli, Rebecca Dickstein, Michael K. Udvardi
    更新日期:2020-01-11
  • Gene Expression in Nitrogen-Fixing Symbiotic Nodule Cells in Medicago truncatula and Other Nodulating Plants
    Plant Cell (IF 8.631) Pub Date : 2020-01-01
    Peter Mergaert, Attila Kereszt, Eva Kondorosi
    更新日期:2020-01-11
  • Phosphorus Availability Regulates TORC1 Signaling via LST8 in Chlamydomonas
    Plant Cell (IF 8.631) Pub Date : 2020-01-01
    Inmaculada Couso, María Esther Pérez-Pérez, Megan M. Ford, Enrique Martínez-Force, Leslie M. Hicks, James G. Umen, José L. Crespo
    更新日期:2020-01-11
  • Candidate Gene Networks for Acylsugar Metabolism and Plant Defense in Wild Tomato Solanum pennellii
    Plant Cell (IF 8.631) Pub Date : 2020-01-01
    Sabyasachi Mandal, Wangming Ji, Thomas D. McKnight
    更新日期:2020-01-11
  • An Improved Recombineering Toolset for Plants
    Plant Cell (IF 8.631) Pub Date : 2020-01-01
    Javier Brumos, Chengsong Zhao, Yan Gong, David Soriano, Arjun P. Patel, Miguel A. Perez-Amador, Anna N. Stepanova, Jose M. Alonso
    更新日期:2020-01-11
  • Advanced Cataloging of Lysine-63 Polyubiquitin Networks by Genomic, Interactome, and Sensor-Based Proteomic Analyses
    Plant Cell (IF 8.631) Pub Date : 2020-01-01
    Natali Romero-Barrios, Dario Monachello, Ulla Dolde, Aloysius Wong, Hélène San Clemente, Anne Cayrel, Alexander Johnson, Claire Lurin, Grégory Vert
    更新日期:2020-01-11
  • Transcriptome-Based Prediction of Complex Traits in Maize
    Plant Cell (IF 8.631) Pub Date : 2020-01-01
    Christina B. Azodi, Jeremy Pardo, Robert VanBuren, Gustavo de los Campos, Shin-Han Shiu
    更新日期:2020-01-11
  • A Large Transposon Insertion in the stiff1 Promoter Increases Stalk Strength in Maize
    Plant Cell (IF 8.631) Pub Date : 2020-01-01
    Zhihai Zhang, Xuan Zhang, Zhelong Lin, Jian Wang, Hangqin Liu, Leina Zhou, Shuyang Zhong, Yan Li, Can Zhu, Jinsheng Lai, Xianran Li, Jianming Yu, Zhongwei Lin
    更新日期:2020-01-11
  • Oxylipins Other Than Jasmonic Acid Are Xylem-Resident Signals Regulating Systemic Resistance Induced by Trichoderma virens in Maize
    Plant Cell (IF 8.631) Pub Date : 2020-01-01
    Ken-Der Wang, Eli J. Borrego, Charles M. Kenerley, Michael V. Kolomiets
    更新日期:2020-01-11
  • PHYTOCHROME INTERACTING FACTOR8 Inhibits Phytochrome A-Mediated Far-Red Light Responses in Arabidopsis
    Plant Cell (IF 8.631) Pub Date : 2020-01-01
    Jeonghwa Oh, Eunae Park, Kijong Song, Gabyong Bae, Giltsu Choi
    更新日期:2020-01-11
  • Arabidopsis ZINC FINGER PROTEIN1 Acts Downstream of GL2 to Repress Root Hair Initiation and Elongation by Directly Suppressing bHLH Genes
    Plant Cell (IF 8.631) Pub Date : 2020-01-01
    Guoliang Han, Xiaocen Wei, Xinxiu Dong, Chengfeng Wang, Na Sui, Jianrong Guo, Fang Yuan, Zhizhong Gong, Xuezhi Li, Yi Zhang, Zhe Meng, Zhuo Chen, Dazhong Zhao, Baoshan Wang
    更新日期:2020-01-11
  • AP2/ERF Transcription Factors Integrate Age and Wound Signals for Root Regeneration
    Plant Cell (IF 8.631) Pub Date : 2020-01-01
    Bin-Bin Ye, Guan-Dong Shang, Yu Pan, Zhou-Geng Xu, Chuan-Miao Zhou, Ying-Bo Mao, Ning Bao, Lijun Sun, Tongda Xu, Jia-Wei Wang
    更新日期:2020-01-10
  • A Jasmonate-Activated MYC2-Dof2.1-MYC2 Transcriptional Loop Promotes Leaf Senescence in Arabidopsis
    Plant Cell (IF 8.631) Pub Date : 2020-01-01
    Mengna Zhuo, Yasuhito Sakuraba, Shuichi Yanagisawa
    更新日期:2020-01-10
  • Arabidopsis SINAT Proteins Control Autophagy by Mediating Ubiquitylation and Degradation of ATG13
    Plant Cell (IF 8.631) Pub Date : 2020-01-01
    Hua Qi, Juan Li, Fan-Nv Xia, Jin-Yu Chen, Xue Lei, Mu-Qian Han, Li-Juan Xie, Qing-Ming Zhou, Shi Xiao
    更新日期:2020-01-10
  • Pathogenic bacteria target plant plasmodesmata to colonize and invade surrounding tissues
    Plant Cell (IF 8.631) Pub Date : 2019-12-30
    Kyaw Aung, Panya Kim, Zhongpeng Li, Anna Joe, Brian H. Kvitko, James R. Alfano, Sheng Yang He

    A hallmark of multicellular organisms is their ability to maintain physiological homeostasis by communicating among cells, tissues, and organs. In plants, intercellular communication is largely dependent on plasmodesmata (PD), which are membrane-lined channels connecting adjacent plant cells. Upon immune stimulation, plants close PD as part of their immune responses. Here, we show that the bacterial pathogen Pseudomonas syringae deploys an effector protein HopO1-1 to modulate the function of PD. HopO1-1 is required for P. syringae to spread locally to neighboring tissues during infection. Expression of HopO1-1 in Arabidopsis increases the distance of PD-dependent molecular flux between neighboring plant cells. Being a putative ribosyltransferease, the catalytic activity of HopO1-1 is required for regulation of PD. HopO1-1 physically interacts with and destabilizes plant PD-located proteins PDLP7 and possibly PDLP5. Both PDLPs are involved in bacterial immunity. Our findings reveal that a pathogenic bacterium utilizes an effector to manipulate PD-mediated host intercellular communication for maximizing the spread of bacterial infection.

    更新日期:2019-12-31
  • Salicylic Acid Suppresses Apical Hook Formation via NPR1-Mediated Repression of EIN3 and EIL1 in Arabidopsis
    Plant Cell (IF 8.631) Pub Date : 2019-12-30
    Peixin Huang, Zhi Dong, Pengru Guo, Xing Zhang, Yuping Qiu, Bosheng Li, Yichuan Wang, Hongwei Guo

    Salicylic acid (SA) and ethylene (ET) are important phytohormones that regulate numerous plant growth, development, and stress response processes. Previous studies have suggested functional interplay of SA and ET in defense responses, but precisely how these two hormones coregulate plant growth and development processes remains unclear. Our present work reveals antagonism between SA and ET in apical hook formation, which ensures successful soil emergence of etiolated dicotyledonous seedlings. Exogenous SA inhibited ET-induced expression of HOOKLESS1 (HLS1) in Arabidopsis thaliana in a manner dependent on ETHYLENE INSENSITIVE3 (EIN3) and EIN3-LIKE1 (EIL1), the core transcription factors in the ET signaling pathway. SA-activated NONEXPRESSER OF PR GENES1 (NPR1) physically interacted with EIN3 and interfered with the binding of EIN3 to target gene promoters, including the HLS1 promoter. Transcriptomic analysis revealed that NPR1 and EIN3/EIL1 coordinately regulated subsets of genes that mediate plant growth and stress responses, suggesting that the interaction between NPR1 and EIN3/EIL1 is an important mechanism for integrating the SA and ET signaling pathways in multiple physiological processes. Taken together, our findings illuminate the molecular mechanism underlying SA regulation of apical hook formation as well as the antagonism between SA and ET in early seedling establishment and possibly other physiological processes.

    更新日期:2019-12-31
  • Metabolite regulatory interactions control plant respiratory metabolism via Target of Rapamycin (TOR) kinase activation
    Plant Cell (IF 8.631) Pub Date : 2019-12-30
    Brendan M O'Leary, Glenda Guek Khim Oh, Chun Pong Lee, A. Harvey Millar

    Respiration rate provides an important readout of energy expenditure and mitochondrial activity in plant cells during the night. As plants inhabit a changing environment, regulatory mechanisms must ensure that respiratory metabolism rapidly and effectively adjusts to the metabolic and environmental conditions of the cell. Using a high-throughput approach we have directly identified specific metabolites which exert transcriptional, translational as well as post-translational control over night-time O2 consumption rate (RN) in mature leaves. Multi-hour RN measurements following leaf disc exposure to a wide array of primary carbon metabolites (carbohydrates, amino acids and organic acids), identified phosphoenolpyruvate (PEP), Pro and Ala as the most potent stimulators of plant leaf RN. Using metabolite combinations we discovered metabolite-metabolite regulatory interactions controlling RN. Many amino acids, as well as glucose analogues, were found to potently inhibit the RN stimulation by Pro and Ala but not PEP. The inhibitory effects of amino acids on Pro and Ala stimulated RN were mitigated by inhibition of the TOR kinase signalling pathway. To confirm the involvement of TOR, these inhibitory amino acids were also shown to be activators of TOR kinase. This provides direct evidence that TOR responds to amino acids by eliciting regulatory effects on respiratory metabolism.

    更新日期:2019-12-31
  • Multiple quality control mechanisms in the ER and TGN determine subcellular dynamics and salt-stress tolerance function of KORRIGAN 1
    Plant Cell (IF 8.631) Pub Date : 2019-12-18
    Yukihiro nagashima, Zeyang Ma, Xueting Liu, Xiaoning Qian, Xiuren Zhang, Antje von Schaewen, Hisashi Koiwa

    Among many glycoproteins within the plant secretory system, KORRIGAN1 (KOR1), a membrane-anchored endo-b1,4-glucanase involved in cellulose biosynthesis, provides a link between N-glycosylation, cell-wall biosynthesis, and abiotic stress tolerance. After insertion into the endoplasmic reticulum (ER), KOR1 cycles between the trans-Golgi network (TGN) and the plasma membrane (PM). From the TGN, the protein is targeted to growing cell plates during cell division. These processes are governed by multiple sequence motifs and also host genotypes. Here we investigated the interaction and hierarchy of known and newly identified sorting signals in KOR1, and how they affect KOR1 transport at various stages in the secretory pathway. Conventional steady-state localization showed that structurally compromised KOR1 variants were directed to tonoplasts. In addition, a newly adapted tandem fluorescent timer (tdFT) technology allowed for differential visualization of young versus aged KOR1 proteins, enabling the analysis of single-pass transport through the secretory pathway. Observations suggest the presence of multiple checkpoints/branches during KOR1 trafficking, where the destination is determined based on KOR1's sequence motifs and folding status. Moreover, growth analyses of dominant PM-confined KOR1-L48L49 -> A48A49 variants revealed the importance of active removal of KOR1 from the PM during salt stress, which otherwise interfered with stress acclimation.

    更新日期:2019-12-19
  • Fusarium virguliforme Transcriptional Plasticity Is Revealed by Host Colonization of Corn vs. Soybean
    Plant Cell (IF 8.631) Pub Date : 2019-12-18
    Amy Baetsen-Young, Ching Man Wai, Robert VanBuren, Brad Day

    We exploited the broad host range of Fusarium virguliforme to identify differential fungal responses leading to either an endophytic or a pathogenic lifestyle during colonization of corn (Zea mays) and soybean (Glycine max), respectively. To provide a foundation to survey the transcriptomic landscape, we produced an improved de novo genome assembly and annotation of F. virguliforme using PacBio sequencing. Next, we conducted a high-resolution time course of F. virguliforme colonization and infection of both soybean, a symptomatic host, and corn, an asymptomatic host. Comparative transcriptomic analyses uncovered a nearly complete network rewiring, with less than 8% average gene coexpression module overlap upon colonizing the different plant hosts. Divergence of transcriptomes originating from host specific temporal induction genes is central to infection and colonization, including carbohydrate-active enzymes (CAZymes) and necrosis inducing effectors. Upregulation of Zn(II)-Cys6 transcription factors were uniquely induced in soybean at 2 days post-inoculation, suggestive of enhanced pathogen virulence on soybean. In total, the data described herein suggest that F. virguliforme modulates divergent infection profiles through transcriptional plasticity.

    更新日期:2019-12-19
  • Mediator Subunit MED25 Couples Alternative Splicing of JAZ Genes with Fine Tuning Jasmonate Signaling
    Plant Cell (IF 8.631) Pub Date : 2019-12-18
    Fangming Wu, Lei Deng, Qingzhe Zhai, Jiuhai Zhao, Qian Chen, Chuanyou Li

    JASMONATE ZIM-DOMAIN (JAZ) transcriptional repressors are key regulators of jasmonate (JA) signaling in plants. At the resting stage, the C-terminal Jas motifs of JAZ proteins bind the transcription factor MYC2 to repress JA signaling. Upon hormone elicitation, the Jas motif binds the hormone receptor COI1, which mediates proteasomal degradation of JAZs and thereby allowing the Mediator subunit MED25 to activate MYC2. Subsequently, plants desensitize JA signaling by feedback generation of dominant JAZ splice variants that repress MYC2. Here we report the mechanistic function of MED25 in regulating the alternative splicing of JAZ genes through recruiting the splicing factors PRE-mRNA-PROCESSING PROTEIN 39a (PRP39a) and PRP40a. We demonstrate that JA-induced generation of JAZ splice variants depends on MED25 and that MED25 recruits PRP39a and PRP40a to promote the full splicing of JAZ genes. Therefore, MED25 forms a module with PRP39a and PRP40a to prevent JAZ splice variants-mediated excessive desensitization of JA signaling.

    更新日期:2019-12-19
  • Diatom Molecular Research Comes of Age: Model Species for Studying Phytoplankton Biology and Diversity
    Plant Cell (IF 8.631) Pub Date : 2019-12-18
    Angela Falciatore, Marianne Jaubert, Jean-Pierre Bouly, Benjamin Bailleul, Thomas Mock

    Diatoms are the most diverse group of algae with at least 100,000 species. Contributing approximately 20% of annual global carbon fixation, they underpin major aquatic food webs and drive global biogeochemical cycles. Over the last two decades, Thalassiosira pseudonana and Phaeodactylum tricornutum have become the most important references for diatom molecular research, ranging from cell biology to ecophysiology, because of their rapid growth rates, small genomes and the cumulative wealth of associated genetic resources. To ascertain the significance of the evolutionary divergence of diatoms, additional references are emerging such as Fragilariopsis cylindrus and Pseudo-nitzschia multistriata. Here, we describe how functional genomics and reverse genetics have contributed to our understanding of this important class of microalgae in the context of evolution, cell biology and metabolic adaptations. Our review will also highlight promising areas of investigation into the diversity of these photosynthetic organisms, including the discovery of new molecular pathways governing the life of secondary plastid-bearing organisms in aquatic environments.

    更新日期:2019-12-19
  • Nup96 and HOS1 are Mutually Stabilized and Gate CONSTANS Protein Level, Conferring Long-day Photoperiodic Flowering Regulation in Arabidopsis
    Plant Cell (IF 8.631) Pub Date : 2019-12-11
    Zhiyuan Cheng, Xiaomei Zhang, Penghui Huang, Guowen Huang, Jinlong Zhu, Fulu Chen, Yuchen Miao, Liangyu Liu, Yongfu Fu, Xu Wang

    Lines of evidence indicate that nuclear pore complex profoundly affects the timing of plant flowering, but the underlying mechanisms remain poorly elucidated. Here, we report that Nucleoporin 96 (Nup96) acts as a negative regulator of long-day photoperiodic flowering in Arabidopsis. Through multiple approaches, we identified and verified an E3 ubiquitin ligase HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENE 1 (HOS1) interacting in vivo with Nup96. Nup96 and HOS1 proteins mainly localize and interact on nuclear membrane. Loss-of-function of Nup96 leads to destruction of HOS1 proteins without changing its mRNA abundance, which results in over-accumulation of the key activator of long-day photoperiodic flowering, CONSTANS (CO) proteins, as that in hos1 mutants. Unexpectedly, we observe that mutation of HOS1 gene strikingly diminishes Nup96 protein level, suggesting that Nup96 and HOS1 are mutually stabilized and thus they form a novel repressive module to regulate CO protein turnover. Therefore, the nup96 and hos1 single and nup96 hos1 double mutants have very much similar early-flowering phenotypes and overlapping transcriptome changes. Together, this study reveals a new repression mechanism, where the Nup96-HOS1 repressive module gates the level of CO proteins, to prevent precocious flowering of Arabidopsis in long-day conditions.

    更新日期:2019-12-13
  • A Sugar Transporter Takes Up both Hexose and Sucrose for Sorbitol-Modulated in vitro Pollen Tube Growth in Apple
    Plant Cell (IF 8.631) Pub Date : 2019-12-11
    Chun-Long Li, Dong Meng, Miguel A Pineros, Yuxin MAO, Abhaya M Dandekar, Lailiang Cheng

    Rapid pollen tube growth requires uptake of sucrose or its hydrolytic products, hexoses, from the apoplast of surrounding tissues in the style. Due to species-specific sugar requirements, reliance of pollen germination and tube growth on cell wall invertase and sucrose or hexose transporters varies between species, but it is not known if there exists a sugar transporter that mediates the uptake of both hexose and sucrose for pollen tube growth. Here, we show that a sugar transporter protein in apple (Malus domestica), MdSTP13a, takes up both hexose and sucrose when expressed in yeast, and is essential for pollen tube growth on glucose and sucrose but not on maltose. MdSTP13a-mediated direct uptake of sucrose is primarily responsible for apple pollen tube growth on sucrose medium. Sorbitol, a major photosynthate and transport carbohydrate in apple, modulates pollen tube growth via a MYB transcription factor, MdMYB39L, which binds to the promoter of MdSTP13a to activate its expression. Antisense repression of MdSTP13a blocks the sorbitol-modulated pollen tube growth. These findings demonstrate that MdSTP13a takes up both hexose and sucrose for sorbitol-modulated pollen tube growth in apple, revealing a situation where acquisition of sugars for pollen tube growth is regulated by a sugar alcohol.

    更新日期:2019-12-13
  • The Ubiquitin-Binding Protein OsDSK2a Mediates Seedling Growth and Salt Responses by Regulating Gibberellin Metabolism in Rice
    Plant Cell (IF 8.631) Pub Date : 2019-12-11
    Juan Wang, Hua Qin, Shi-Rong Zhou, Pengcheng Wei, Haiwen Zhang, Yun Zhou, Yuchen Miao, Rongfeng Huang

    UBL-UBA (ubiquitin-like-ubiquitin-associated) proteins are ubiquitin receptors and transporters in the ubiquitin-proteasome system that play key roles in plant growth and development. High salinity restricts plant growth by disrupting cellular metabolism, but whether UBL-UBA proteins are involved in this process is unclear. Here, we demonstrate that the novel UBL-UBA protein OsDSK2a (DOMINANT SUPPRESSOR of KAR2) mediates seedling growth and salt responses in rice (Oryza sativa). Through analysis of osdsk2a, a mutant with retarded seedling growth, as well as in vitro and in vivo assays, we demonstrated that OsDSK2a combines with polyubiquitin chains and interacts with the gibberellin (GA)-deactivating enzyme ELONGATED UPPERMOST INTERNODE (EUI), resulting in its degradation through the ubiquitin-proteasome system. Bioactive GA levels were reduced and plant growth was retarded in the osdsk2a mutant. By contrast, eui mutants displayed increased seedling growth and bioactive GA levels. OsDSK2a levels decreased in plant under salt stress. Moreover, EUI accumulated under salt stress more rapidly in osdsk2a than in wild-type plants. Thus, OsDSK2a and EUI play opposite roles in regulating plant growth under salt stress by affecting GA metabolism. Downregulation of the ubiquitin-binding receptor OsDSK2a increases EUI accumulation, which promotes GA metabolism and reduces plant growth under salt stress.

    更新日期:2019-12-13
  • Mightier Than Muscle: A Near-Atomic View of Pollen Actin Filaments
    Plant Cell (IF 8.631) Pub Date : 2019-12-01
    Kathleen L. Farquharson
    更新日期:2019-12-11
  • 更新日期:2019-12-11
  • You Are What You Eat: An ATG1-Independent Path to Autophagy
    Plant Cell (IF 8.631) Pub Date : 2019-12-01
    Robert C. Augustine
    更新日期:2019-12-11
  • Attract or Defend: The CYP-Associated Versatility of Terpenoids
    Plant Cell (IF 8.631) Pub Date : 2019-12-01
    Céline Caseys
    更新日期:2019-12-11
  • 更新日期:2019-12-11
  • Local Manufacturing: A Center for Photosystem Biogenesis
    Plant Cell (IF 8.631) Pub Date : 2019-12-01
    Estee E. Tee
    更新日期:2019-12-11
  • 更新日期:2019-12-11
  • Keeping an Eye on Lutein Stability
    Plant Cell (IF 8.631) Pub Date : 2019-12-01
    Gregory Bertoni
    更新日期:2019-12-11
  • Introducing CRISPR-TSKO: A Breakthrough in Precision Gene Editing
    Plant Cell (IF 8.631) Pub Date : 2019-12-01
    Jennifer Lockhart
    更新日期:2019-12-11
  • Remove, Recycle, Degrade: Regulating Plasma Membrane Protein Accumulation
    Plant Cell (IF 8.631) Pub Date : 2019-12-01
    Cecilia Rodriguez-Furlan, Elena A. Minina, Glenn R. Hicks
    更新日期:2019-12-11
  • Cryo-EM Structure of Actin Filaments from Zea mays Pollen
    Plant Cell (IF 8.631) Pub Date : 2019-12-01
    Zhanhong Ren, Yan Zhang, Yi Zhang, Yunqiu He, Pingzhou Du, Zhanxin Wang, Fei Sun, Haiyun Ren
    更新日期:2019-12-11
  • CRISPR-TSKO: A Technique for Efficient Mutagenesis in Specific Cell Types, Tissues, or Organs in Arabidopsis
    Plant Cell (IF 8.631) Pub Date : 2019-12-01
    Ward Decaestecker, Rafael Andrade Buono, Marie L. Pfeiffer, Nick Vangheluwe, Joris Jourquin, Mansour Karimi, Gert Van Isterdael, Tom Beeckman, Moritz K. Nowack, Thomas B. Jacobs
    更新日期:2019-12-11
  • The Transcriptional Landscape of Polyploid Wheats and Their Diploid Ancestors during Embryogenesis and Grain Development
    Plant Cell (IF 8.631) Pub Date : 2019-12-01
    Daoquan Xiang, Teagen D. Quilichini, Ziying Liu, Peng Gao, Youlian Pan, Qiang Li, Kirby T. Nilsen, Prakash Venglat, Eddi Esteban, Asher Pasha, Yejun Wang, Rui Wen, Zhongjuan Zhang, Zhaodong Hao, Edwin Wang, Yangdou Wei, Richard Cuthbert, Leon V. Kochian, Andrew Sharpe, Nicholas Provart, Dolf Weijers, C. Stewart Gillmor, Curtis Pozniak, Raju Datla
    更新日期:2019-12-11
  • Depletion of the FtsH1/3 Proteolytic Complex Suppresses the Nutrient Stress Response in the Cyanobacterium Synechocystis sp strain PCC 6803
    Plant Cell (IF 8.631) Pub Date : 2019-12-01
    Vendula Krynická, Jens Georg, Philip J. Jackson, Mark J. Dickman, C. Neil Hunter, Matthias E. Futschik, Wolfgang R. Hess, Josef Komenda
    更新日期:2019-12-11
  • MicroRNA Dynamics and Functions During Arabidopsis Embryogenesis
    Plant Cell (IF 8.631) Pub Date : 2019-12-01
    Alexandra Plotnikova, Max J. Kellner, Michael A. Schon, Magdalena Mosiolek, Michael D. Nodine
    更新日期:2019-12-11
  • A Promiscuous CYP706A3 Reduces Terpene Volatile Emission from Arabidopsis Flowers, Affecting Florivores and the Floral Microbiome
    Plant Cell (IF 8.631) Pub Date : 2019-12-01
    Benoît Boachon, Yannick Burdloff, Ju-Xin Ruan, Rakotoharisoa Rojo, Robert R. Junker, Bruno Vincent, Florence Nicolè, Françoise Bringel, Agnès Lesot, Laura Henry, Jean-Etienne Bassard, Sandrine Mathieu, Lionel Allouche, Ian Kaplan, Natalia Dudareva, Stéphane Vuilleumier, Laurence Miesch, François André, Nicolas Navrot, Xiao-Ya Chen, Danièle Werck-Reichhart
    更新日期:2019-12-11
  • Genetic Analyses of the Arabidopsis ATG1 Kinase Complex Reveal Both Kinase-Dependent and Independent Autophagic Routes during Fixed-Carbon Starvation
    Plant Cell (IF 8.631) Pub Date : 2019-12-01
    Xiao Huang, Chunyan Zheng, Fen Liu, Chao Yang, Ping Zheng, Xing Lu, Jiang Tian, Taijoon Chung, Marisa S. Otegui, Shi Xiao, Caiji Gao, Richard D. Vierstra, Faqiang Li
    更新日期:2019-12-11
  • ORANGE Represses Chloroplast Biogenesis in Etiolated Arabidopsis Cotyledons via Interaction with TCP14
    Plant Cell (IF 8.631) Pub Date : 2019-12-01
    Tianhu Sun, Fei Zhou, Xing-Qi Huang, Wei-Cai Chen, Meng-Juan Kong, Chang-Fang Zhou, Zhong Zhuang, Li Li, Shan Lu
    更新日期:2019-12-11
  • Secretion of Phospholipase D{delta} Functions as a Regulatory Mechanism in Plant Innate Immunity
    Plant Cell (IF 8.631) Pub Date : 2019-12-01
    Jingjing Xing, Xiaojuan Li, Xiaohua Wang, Xueqin Lv, Li Wang, Liang Zhang, Yingfang Zhu, Qianhua Shen, František Baluška, Jozef Šamaj, Jinxing Lin
    更新日期:2019-12-11
  • Divergent Functional Diversification Patterns in the SEP/AGL6/AP1 MADS-Box Transcription Factor Superclade
    Plant Cell (IF 8.631) Pub Date : 2019-12-01
    Patrice Morel, Pierre Chambrier, Véronique Boltz, Sophy Chamot, Frédérique Rozier, Suzanne Rodrigues Bento, Christophe Trehin, Marie Monniaux, Jan Zethof, Michiel Vandenbussche
    更新日期:2019-12-11
  • Photosystem Biogenesis Is Localized to the Translation Zone in the Chloroplast of Chlamydomonas
    Plant Cell (IF 8.631) Pub Date : 2019-12-01
    Yi Sun, Melissa Valente-Paterno, Shiva Bakhtiari, Christopher Law, Yu Zhan, William Zerges
    更新日期:2019-12-11
  • Dual Activities of Plant cGMP-Dependent Protein Kinase and Its Roles in Gibberellin Signaling and Salt Stress
    Plant Cell (IF 8.631) Pub Date : 2019-12-01
    Qingwen Shen, Xinqiao Zhan, Pei Yang, Jing Li, Jie Chen, Bing Tang, Xuemin Wang, Yueyun Hong
    更新日期:2019-12-11
  • A GDSL Esterase/Lipase Catalyzes the Esterification of Lutein in Bread Wheat
    Plant Cell (IF 8.631) Pub Date : 2019-12-01
    Jacinta L. Watkins, Ming Li, Ryan P. McQuinn, Kai Xun Chan, Heather E. McFarlane, Maria Ermakova, Robert T Furbank, Daryl Mares, Chongmei Dong, Kenneth J. Chalmers, Peter Sharp, Diane E. Mather, Barry J. Pogson
    更新日期:2019-12-11
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