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Vacuolar Degradation of Plant Organelles Plant Cell (IF 11.6) Pub Date : 2024-04-24 Marisa S Otegui, Charlotte Steelheart, Wenlong Ma, Juncai Ma, Byung-Ho Kang, Victor Sanchez De Medina Hernandez, Yasin Dagdas, Caiji Gao, Shino Goto-Yamada, Kazusato Oikawa, Mikio Nishimura
Plants continuously remodel and degrade their organelles due to damage from their metabolic activities and environmental stressors, as well as an integral part of their cell differentiation programs. Whereas certain organelles use local hydrolytic enzymes for limited remodeling, most of pathways that control the partial or complete dismantling of organelles rely on vacuolar degradation. Specifically
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Cytochrome b5 diversity in green lineages preceded the evolution of syringyl lignin biosynthesis Plant Cell (IF 11.6) Pub Date : 2024-04-24 Xianhai Zhao, Yunjun Zhao, Qing-yin Zeng, Chang-Jun Liu
Lignin production marked a milestone in vascular plant evolution, and the emergence of syringyl (S)-lignin is lineage-specific. S-lignin biosynthesis in angiosperms, mediated by ferulate 5-hydroxylase (F5H, CYP84A1), has been considered a recent evolutionary event. F5H uniquely requires the cytochrome b5 protein CB5D as an obligatory redox partner for catalysis. However, it remains unclear how CB5D
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Erasing marks: Functions of plant deubiquitylating enzymes in modulating the ubiquitin code Plant Cell (IF 11.6) Pub Date : 2024-04-24 Karin Vogel, Erika Isono
Plant cells need to respond to environmental stimuli and developmental signals accurately and promptly. Ubiquitylation is a reversible posttranslational modification that enables the adaptation of cellular proteostasis to internal or external factors. The different topologies of ubiquitin linkages serve as the structural basis for the ubiquitin code, which can be interpreted by ubiquitin-binding proteins
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The structural basis for light harvesting in organisms producing phycobiliproteins Plant Cell (IF 11.6) Pub Date : 2024-04-23 Donald A Bryant, Christopher J Gisriel
Cyanobacteria, red algae, and cryptophytes produce two classes of proteins for light-harvesting: water-soluble phycobiliproteins and membrane-intrinsic proteins that bind chlorophylls and carotenoids. In cyanobacteria, red algae, and glaucophytes, phycobilisomes (PBS) are complexes of brightly colored phycobiliproteins and linker (assembly) proteins. To date, six structural classes of phycobilisomes
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The H3K4 demethylase JMJ1 is required for proper timing of flowering in Brachypodium distachyon Plant Cell (IF 11.6) Pub Date : 2024-04-23 Bing Liu, Chengzhang Li, Xiang Li, Jiachen Wang, Wenhao Xie, Daniel P Woods, Weiya Li, Xiaoyu Zhu, Shuoming Yang, Aiwu Dong, Richard M Amasino
Flowering is a key developmental transition in the plant life cycle. In temperate climates, flowering often occurs in response to the perception of seasonal cues such as changes in day-length and temperature. However, the mechanisms that have evolved to control the timing of flowering in temperate grasses are not fully understood. We identified a Brachypodium distachyon mutant whose flowering is delayed
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Protein degradation in the auxin response Plant Cell (IF 11.6) Pub Date : 2024-04-23 Martijn de Roij, Jan Willem Borst, Dolf Weijers
The signaling molecule auxin sits at the nexus of plant biology and coordinates essentially all growth and developmental processes in plants. Auxin molecules are transported throughout plant tissues and are capable of evoking highly specific physiological responses in plant cells by inducing various molecular pathways. In many of these pathways, proteolysis plays a crucial role for correct physiological
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Arabidopsis class A S-acyl transferases modify the pollen receptors LIP1 and PRK1 to regulate pollen tube guidance Plant Cell (IF 11.6) Pub Date : 2024-04-18 Xiaojiao Xiang, Zhiyuan Wan, Shuzhan Zhang, Qiang-Nan Feng, Shan-Wei Li, Gui-Min Yin, Jing-Yu Zhi, Xin Liang, Ting Ma, Sha Li, Yan Zhang
Protein S-acylation catalyzed by protein S-acyl transferases (PATs) is a reversible lipid modification regulating protein targeting, stability, and interaction profiles. PATs are encoded by large gene families in plants, and many proteins including receptor-like cytoplasmic kinases (RLCKs) and receptor-like kinases (RLKs) are subject to S-acylation. However, few PATs have been assigned substrates,
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The transcriptome landscape of developing barley seeds Plant Cell (IF 11.6) Pub Date : 2024-04-18 Martin Kovacik, Anna Nowicka, Jana Zwyrtková, Beáta Strejčková, Isaia Vardanega, Eddi Esteban, Asher Pasha, Kateřina Kaduchová, Maryna Krautsova, Marie Červenková, Jan Šafář, Nicholas J Provart, Rüdiger Simon, Ales Pecinka
Cereal grains are an important source of food and feed. To provide comprehensive spatiotemporal information about biological processes in developing seeds of cultivated barley (Hordeum vulgare L. subsp. vulgare), we performed a transcriptomic study of the embryo, endosperm, and seed maternal tissues collected from grains 4–32 days after pollination. Weighted gene co-expression network and motif enrichment
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The GRAS transcription factor CsTL regulates tendril formation in cucumber Plant Cell (IF 11.6) Pub Date : 2024-04-17 Junjun Shen, Yanxin Jiang, Jian Pan, Linhan Sun, Qingqing Li, Wenjing He, Piaoyun Sun, Bosi Zhao, Hongjiao Zhao, Xubo Ke, Yalu Guo, Tongwen Yang, Zheng Li
Cucumber (Cucumis sativus, Cs) tendrils are slender vegetative organs that typically require manual removal to ensure orderly growth during greenhouse cultivation. Here, we identified cucumber tendril-less (tl), a Tnt1 retrotransposon-induced insertion mutant lacking tendrils. Map-based cloning identified the mutated gene, CsaV3_3G003590, which we designated as CsTL, which is homologous to Arabidopsis
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BIG enhances Arg/N-degron pathway-mediated protein degradation to regulate Arabidopsis hypoxia responses and suberin deposition Plant Cell (IF 11.6) Pub Date : 2024-04-12 Hongtao Zhang, Chelsea Rundle, Nikola Winter, Alexandra Miricescu, Brian C Mooney, Andreas Bachmair, Emmanuelle Graciet, Frederica L Theodoulou
BIG/DARK OVEREXPRESSION OF CAB1/TRANSPORT INHIBITOR RESPONSE3 is a 0.5-MDa protein associated with multiple functions in Arabidopsis (Arabidopsis thaliana) signalling and development. However, the biochemical functions of BIG are unknown. We investigated a role for BIG in the Arg/N-degron pathways, in which substrate protein fate is influenced by the N-terminal (Nt) residue. We crossed a big loss-of-function
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The N-terminal domains of NLR immune receptors exhibit structural and functional similarities across divergent plant lineages Plant Cell (IF 11.6) Pub Date : 2024-04-10 Khong-Sam Chia, Jiorgos Kourelis, Albin Teulet, Martin Vickers, Toshiyuki Sakai, Joseph F Walker, Sebastian Schornack, Sophien Kamoun, Philip Carella
Nucleotide-binding domain and leucine-rich repeat (NLR) proteins are a prominent class of intracellular immune receptors in plants. However, our understanding of plant NLR structure and function is limited to the evolutionarily young flowering plant clade. Here, we describe an extended spectrum of NLR diversity across divergent plant lineages and demonstrate the structural and functional similarities
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The Y locus encodes a REPRESSOR OF PHOTOSYNTHETIC GENES protein that represses carotenoid biosynthesis via interaction with APRR2 in carrot Plant Cell (IF 11.6) Pub Date : 2024-04-09 Ying-Gang Wang, Yu-Min Zhang, Ya-Hui Wang, Kai Zhang, Jing Ma, Jia-Xin Hang, Yu-Ting Su, Shan-Shan Tan, Hui Liu, Ai-Sheng Xiong, Zhi-Sheng Xu
Little is known about the factors regulating carotenoid biosynthesis in roots. In this study, we characterized DCAR_032551, the candidate gene of the Y locus responsible for the transition of root color from ancestral white to yellow during carrot (Daucus carota) domestication. We show that DCAR_032551 encodes a REPRESSOR OF PHOTOSYNTHETIC GENES (RPGE) protein, named DcRPGE1. DcRPGE1 from wild carrot
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The phytochrome-interacting factor genes PIF1 and PIF4 are functionally diversified due to divergence of promoters and proteins Plant Cell (IF 11.6) Pub Date : 2024-04-09 Hanim Kim, Nayoung Lee, Yeojae Kim, Giltsu Choi
Phytochrome-interacting factors (PIFs) are basic helix-loop-helix transcription factors that regulate light responses downstream of phytochromes. In Arabidopsis (Arabidopsis thaliana), eight PIFs (PIF1-8) regulate light responses, either redundantly or distinctively. Distinctive roles of PIFs may be attributed to differences in mRNA expression patterns governed by promoters or variations in molecular
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Translational activation by a synthetic PPR protein elucidates control of psbA translation in Arabidopsis chloroplasts Plant Cell (IF 11.6) Pub Date : 2024-04-09 Margarita Rojas, Prakitchai Chotewutmontri, Alice Barkan
Translation initiation on chloroplast psbA mRNA in plants scales with light intensity, providing its gene product, D1, as needed to replace photodamaged D1 in Photosystem II. The psbA translational activator HIGH CHLOROPHYLL FLUORESCENCE 173 (HCF173) has been hypothesized to mediate this regulation. HCF173 belongs to the short-chain dehydrogenase/reductase superfamily, associates with the psbA 5'-untranslated
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Arabidopsis transcription factor TCP4 controls the identity of the apical gynoecium Plant Cell (IF 11.6) Pub Date : 2024-04-06 Yutao Wang, Ning Wang, Jingqiu Lan, Yige Pan, Yidan Jiang, Yongqi Wu, Xuemei Chen, Xianzhong Feng, Genji Qin
The style and stigma at the apical gynoecium are crucial for flowering plant reproduction. However, the mechanisms underlying specification of the apical gynoecium remain unclear. Here, we demonstrate that Class II TEOSINTE BRANCHED 1/CYCLOIDEA/PCF (TCP) transcription factors are critical for apical gynoecium specification in Arabidopsis (Arabidopsis thaliana). The septuple tcp2 tcp3 tcp4 tcp5 tcp10
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The AGAMOUS-LIKE 16–GENERAL REGULATORY FACTOR 1 module regulates axillary bud outgrowth via catabolism of abscisic acid in cucumber Plant Cell (IF 11.6) Pub Date : 2024-04-06 Jiacai Chen, Liu Liu, Guanghui Wang, Guangxin Chen, Xiaofeng Liu, Min Li, Lijie Han, Weiyuan Song, Shaoyun Wang, Chuang Li, Zhongyi Wang, Yuxiang Huang, Chaoheng Gu, Zhengan Yang, Zhaoyang Zhou, Jianyu Zhao, Xiaolan Zhang
Lateral branches are important components of shoot architecture and directly affect crop yield and production cost. Although sporadic studies have implicated abscisic acid (ABA) biosynthesis in axillary bud outgrowth, the function of ABA catabolism and its upstream regulators in shoot branching remain elusive. Here, we showed that the MADS-box transcription factor AGAMOUS-LIKE 16 (CsAGL16) is a positive
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The Heat shock factor 20–HSF4–Cellulose synthase A2 module regulates heat stress tolerance in maize Plant Cell (IF 11.6) Pub Date : 2024-04-04 Ze Li, Zerui Li, Yulong Ji, Chunyu Wang, Shufang Wang, Yiting Shi, Jie Le, Mei Zhang
Temperature shapes the geographical distribution and behavior of plants. Understanding the regulatory mechanisms underlying the plant heat response is important for developing climate-resilient crops, including maize (Zea mays). To identify transcription factors that may contribute to the maize heat response, we generated a dataset of short- and long-term transcriptome changes following a heat treatment
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Structure, biogenesis and evolution of thylakoid membranes Plant Cell (IF 11.6) Pub Date : 2024-04-03 Matthias Ostermeier, Adriana Garibay-Hernández, Victoria J C Holzer, Michael Schroda, Jörg Nickelsen
Cyanobacteria and chloroplasts of algae and plants harbor specialized thylakoid membranes that convert sunlight into chemical energy. These membranes house photosystems II and I, the vital protein-pigment complexes that drive oxygenic photosynthesis. In the course of their evolution, thylakoid membranes have diversified in structure. However, the core machinery for photosynthetic electron transport
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The S-acylation cycle of transcription factor MtNAC80 influences cold stress responses in Medicago truncatula Plant Cell (IF 11.6) Pub Date : 2024-03-29 Qinyi Ye, Lihua Zheng, Peng Liu, Qianwen Liu, Tuo Ji, Jinling Liu, Yajuan Gao, Li Liu, Jiangli Dong, Tao Wang
S-acylation is a reversible post-translational modification catalyzed by protein S-acyltransferases (PATs), and acyl protein thioesterases (APTs) mediate de-S-acylation. Although many proteins are S-acylated, how the S-acylation cycle modulates specific biological functions in plants is poorly understood. In this study, we report that the S-acylation cycle of transcription factor MtNAC80 is involved
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Transcription factor FveMYB117a inhibits axillary bud outgrowth by regulating cytokinin homeostasis in woodland strawberry Plant Cell (IF 11.6) Pub Date : 2024-03-28 Yafan Han, Minghao Qu, Zhongchi Liu, Chunying Kang
Shoot branching affects plant architecture. In strawberry (Fragaria L.), short branches (crowns) develop from dormant axillary buds to form inflorescences and flowers. While this developmental transition contributes greatly to perenniality and yield in strawberry, its regulatory mechanism remains unclear and understudied. In the woodland strawberry (Fragaria vesca), we identified and characterized
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Plastid retrograde signaling: A developmental perspective Plant Cell (IF 11.6) Pub Date : 2024-03-28 Naresh Loudya, Alice Barkan, Enrique López-Juez
Chloroplast activities influence nuclear gene expression, a phenomenon referred to as retrograde signaling. Biogenic retrograde signals have been revealed by changes in nuclear gene expression when chloroplast development is disrupted. Research on biogenic signaling has focused on repression of Photosynthesis Associated Nuclear Genes (PhANGs) but this is just one component of a syndrome involving altered
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Autophagosome biogenesis and organelle homeostasis in plant cells Plant Cell (IF 11.6) Pub Date : 2024-03-27 Xiaohong Zhuang, Baiying Li, Liwen Jiang
Autophagy is one of the major highly inducible degradation processes in response to plant developmental and environmental signals. In response to different stimuli, cellular materials, including proteins and organelles, can be sequestered into a double membrane autophagosome structure either selectively or non-selectively. The formation of an autophagosome as well as its delivery into the vacuole involves
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INDUCER OF CBF EXPRESSION 1 promotes cold-enhanced immunity by directly activating salicylic acid signaling Plant Cell (IF 11.6) Pub Date : 2024-03-27 Shaoqin Li, Li He, Yongping Yang, Yixin Zhang, Xiao Han, Yanru Hu, Yanjuan Jiang
Cold stress affects plant immune responses, and this process may involve the salicylic acid (SA) signaling pathway. However, the underlying mechanism by which low temperature signals coordinate with SA signaling to regulate plant immunity remains unclear. Here, we found that low temperatures enhanced the disease resistance of Arabidopsis thaliana against Pseudomonas syringae pv. tomato (Pst) DC3000
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H3K4me1 recruits DNA repair proteins in plants Plant Cell (IF 11.6) Pub Date : 2024-03-27 Daniela Quiroz, Satoyo Oya, Diego Lopez-Mateos, Kehan Zhao, Alice Pierce, Lissandro Ortega, Alissza Ali, Pablo Carbonell-Bejerano, Vladimir Yarov-Yarovoy, Sae Suzuki, Gosuke Hayashi, Akihisa Osakabe, J Grey Monroe
DNA repair proteins can be recruited by their histone reader domains to specific epigenomic features, with consequences on intragenomic mutation rate variation. Here, we investigated H3K4me1-associated hypomutation in plants. We first examined two proteins which, in plants, contain Tudor histone reader domains: PRECOCIOUS DISSOCIATION OF SISTERS 5 (PDS5C), involved in homology-directed repair, and
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The adaptor protein ECAP, the co-repressor LEUNIG, and the transcription factor BEH3 interact and regulate microsporocyte generation in Arabidopsis Plant Cell (IF 11.6) Pub Date : 2024-03-25 Lei Shi, Changjiang Li, Gaofeng Lv, Xing Li, Wutao Feng, Yujing Bi, Wenhui Wang, Youqun Wang, Lei Zhu, Wenqiang Tang, Ying Fu
Histo-specification and morphogenesis of anthers during development in Arabidopsis (Arabidopsis thaliana) are well understood. However, the regulatory mechanism of microsporocyte generation at the pre-meiotic stage remains unclear, especially how archesporial cells are specified and differentiate into two cell lineages with distinct developmental fates. SPOROCYTELESS (SPL) is a key reproductive gene
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Arginine methylation of SM-LIKE PROTEIN 4 antagonistically affects alternative splicing during Arabidopsis stress responses Plant Cell (IF 11.6) Pub Date : 2024-03-22 Yamila Carla Agrofoglio, María José Iglesias, Soledad Perez-Santángelo, María José de Leone, Tino Koester, Rafael Catalá, Julio Salinas, Marcelo J Yanovsky, Dorothee Staiger, Julieta L Mateos
Arabidopsis (Arabidopsis thaliana) PROTEIN ARGININE METHYLTRANSFERASE5 (PRMT5) post-translationally modifies RNA-binding proteins by arginine (R) methylation. However, the impact of this modification on the regulation of RNA processing is largely unknown. We used the spliceosome component, SM-LIKE PROTEIN 4 (LSM4), as a paradigm to study the role of R-methylation in RNA processing. We found that LSM4
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CONSTANS, a HUB for all seasons: How photoperiod pervades plant physiology regulatory circuits Plant Cell (IF 11.6) Pub Date : 2024-03-21 Jose M Romero, Gloria Serrano-Bueno, Carolina Camacho-Fernández, Mateus Henrique Vicente, M Teresa Ruiz, J Román Pérez-Castiñeira, Javier Pérez-Hormaeche, Fabio T S Nogueira, Federico Valverde
How does a plant detect the changing seasons and make important developmental decisions accordingly? How do they incorporate daylength information into their routine physiological processes? Photoperiodism, or the capacity to measure the daylength, is a crucial aspect of plant development that helps plants determine the best time of the year to make vital decisions, such as flowering. The protein CONSTANS
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Plant enhancers exhibit both cooperative and additive interactions among their functional elements Plant Cell (IF 11.6) Pub Date : 2024-03-21 Tobias Jores, Jackson Tonnies, Nicholas A Mueth, Andrés Romanowski, Stanley Fields, Josh T Cuperus, Christine Queitsch
Enhancers are cis-regulatory elements that shape gene expression in response to numerous developmental and environmental cues. In animals, several models have been proposed to explain how enhancers integrate the activity of multiple transcription factors. However, it remains largely unclear how plant enhancers integrate transcription factor activity. Here, we use Plant STARR-seq to characterize three
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The dimorphic diaspore model Aethionema arabicum (Brassicaceae): Distinct molecular and morphological control of responses to parental and germination temperatures Plant Cell (IF 11.6) Pub Date : 2024-03-21 Jake O Chandler, Per K I Wilhelmsson, Noe Fernandez-Pozo, Kai Graeber, Waheed Arshad, Marta Pérez, Tina Steinbrecher, Kristian K Ullrich, Thu-Phuong Nguyen, Zsuzsanna Mérai, Klaus Mummenhoff, Günter Theißen, Miroslav Strnad, Ortrun Mittelsten Scheid, M Eric Schranz, Ivan Petřík, Danuše Tarkowská, Ondřej Novák, Stefan A Rensing, Gerhard Leubner-Metzger
Plants in habitats with unpredictable conditions often have diversified bet-hedging strategies that ensure fitness over a wider range of variable environmental factors. A striking example is the diaspore (seed and fruit) heteromorphism that evolved to maximize species survival in Aethionema arabicum (Brassicaceae) in which external and endogenous triggers allow the production of two distinct diaspores
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What is going on inside of phytochrome B photobodies? Plant Cell (IF 11.6) Pub Date : 2024-03-21 Björn C Willige, Chan Yul Yoo, J Paola Saldierna Guzmán
Plants exhibit an enormous phenotypic plasticity to adjust to changing environmental conditions. For this purpose, they have evolved mechanisms to detect and measure biotic and abiotic factors in their surroundings. Phytochrome B exhibits a dual function, since it serves as a photoreceptor for red and far-red light as well as a thermosensor. In 1999, it was first reported that phytochromes not only
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Arabidopsis ribosomal RNA processing meerling mutants exhibit suspensor-derived polyembryony due to direct reprogramming of the suspensor Plant Cell (IF 11.6) Pub Date : 2024-03-21 Honglei Wang, Luca Santuari, Tristan Wijsman, Guy Wachsman, Hannah Haase, Michael Nodine, Ben Scheres, Renze Heidstra
Embryo development in Arabidopsis (Arabidopsis thaliana) starts off with an asymmetric division of the zygote to generate the precursors of the embryo proper and the supporting extraembryonic suspensor. The suspensor degenerates as the development of the embryo proper proceeds beyond the heart stage. Until the globular stage, the suspensor maintains embryonic potential and can form embryos in the absence
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The OsEIL1–OsWOX11 transcription factor module controls rice crown root development in response to soil compaction Plant Cell (IF 11.6) Pub Date : 2024-03-15 Yuxiang Li, Juan Wang, Yadi Gao, Bipin K Pandey, Lucas León Peralta Ogorek, Yu Zhao, Ruidang Quan, Zihan Zhao, Lei Jiang, Rongfeng Huang, Hua Qin
Optimizing the root architecture of crops is an effective strategy for improving crop yields. Soil compaction is a serious global problem that limits crop productivity by restricting root growth, but the underlying molecular mechanisms are largely unclear. Here, we show that ethylene stimulates rice (Oryza sativa) crown root development in response to soil compaction. First, we demonstrate that compacted
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The biogenesis and maintenance of photosystem II: recent advances and current challenges Plant Cell (IF 11.6) Pub Date : 2024-03-14 Josef Komenda, Roman Sobotka, Peter J Nixon
The growth of plants, algae and cyanobacteria relies on the catalytic activity of the oxygen-evolving photosystem two (PSII) complex which uses solar energy to extract electrons from water to feed into the photosynthetic electron transport chain. PSII is proving to be an excellent system to study how large multi-subunit membrane-protein complexes are assembled in the thylakoid membrane and subsequently
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Chloroplast ATP synthase: from structure to engineering Plant Cell (IF 11.6) Pub Date : 2024-03-14 Thilo Rühle, Dario Leister, Viviana Pasch
F-type ATP synthases are extensively researched protein complexes because of their widespread and central role in energy metabolism. Progress in structural biology, proteomics, and molecular biology has also greatly advanced our understanding of the catalytic mechanism, post-translational modifications, and biogenesis of chloroplast ATP synthases. Given their critical role in light-driven ATP generation
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A gradient of the HD-Zip regulator Woolly regulates multicellular trichome morphogenesis in tomato Plant Cell (IF 11.6) Pub Date : 2024-03-12 MinLiang Wu, XinXin Bian, ShouRong Hu, BenBen Huang, JingYuan Shen, YaDi Du, YanLi Wang, MengYuan Xu, HuiMin Xu, MeiNa Yang, Shuang Wu
Homeodomain (HD) proteins regulate embryogenesis in animals such as the fruit fly (Drosophila melanogaster), often in a concentration-dependent manner. HD-leucine zipper (Zip) IV family genes are unique to plants and often function in the L1 epidermal cell layer. However, our understanding of the roles of HD-Zip IV family genes in plant morphogenesis is limited. In this study, we investigated the morphogenesis
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Evidence for an RNAi-independent role of Arabidopsis DICER-LIKE2 in growth inhibition and basal antiviral resistance Plant Cell (IF 11.6) Pub Date : 2024-03-11 Carsten Poul Skou Nielsen, Laura Arribas-Hernández, Lijuan Han, Marlene Reichel, Jakob Woessmann, Rune Daucke, Simon Bressendorff, Diego López-Márquez, Stig Uggerhøj Andersen, Nathan Pumplin, Erwin M Schoof, Peter Brodersen
Flowering plant genomes encode four or five DICER-LIKE (DCL) enzymes that produce small interfering RNAs (siRNAs) and microRNAs which function in RNA interference (RNAi). Different RNAi pathways in plants effect transposon silencing, antiviral defense and endogenous gene regulation. DCL2 acts genetically redundantly with DCL4 to confer basal antiviral defense. However, DCL2 may also counteract DCL4
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Proteolytic control of the RNA silencing machinery Plant Cell (IF 11.6) Pub Date : 2024-03-08 Pascal Genschik, Marlene Schiaffini, Esther Lechner
Studies in plants were often pioneering in the field of RNA silencing and revealed a broad range of small RNA (sRNA) categories. When associated with ARGONAUTE (AGO) proteins, sRNAs play important functions in development, genome integrity, stress responses, and antiviral immunity. Today, most of the protein factors required for the biogenesis of sRNA classes, their amplification through the production
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ENHANCER OF SHOOT REGENERATION1 promotes de novo root organogenesis after wounding in Arabidopsis leaf explants Plant Cell (IF 11.6) Pub Date : 2024-03-06 Kyounghee Lee, Hobin Yoon, Ok-Sun Park, Pil Joon Seo
Plants have an astonishing ability to regenerate new organs after wounding. Here, we report that the wound-inducible transcription factor ENHANCER OF SHOOT REGENERATION1 (ESR1) has a dual mode of action in activating ANTHRANILATE SYNTHASE ALPHA SUBUNIT1 (ASA1) expression to ensure auxin-dependent de novo root organogenesis locally at wound sites of Arabidopsis (Arabidopsis thaliana) leaf explants.
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Rapid depletion of target proteins in plants by an inducible protein degradation system Plant Cell (IF 11.6) Pub Date : 2024-03-06 Linzhou Huang, Marcela Rojas-Pierce
Inducible protein knockdowns are excellent tools to test the function of essential proteins in short time scales and to capture the role of proteins in dynamic events. Current approaches destroy or sequester proteins by exploiting plant biological mechanisms such as the activity of photoreceptors for optogenetics or auxin-mediated ubiquitination in auxin degrons. It follows that these are not applicable
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Heat-induced SUMOylation differentially affects bacterial effectors in plant cells Plant Cell (IF 11.6) Pub Date : 2024-03-06 Wenliang Li, Wen Liu, Zewei Xu, Chengluo Zhu, Danlu Han, Jianwei Liao, Kun Li, Xiaoyan Tang, Qi Xie, Chengwei Yang, Jianbin Lai
Bacterial pathogens deliver effectors into host cells to suppress immunity. How host cells target these effectors is critical in pathogen–host interactions. SUMOylation, an important type of posttranslational modification in eukaryotic cells, plays a critical role in immunity, but its effect on bacterial effectors remains unclear in plant cells. In this study, using bioinformatic and biochemical approaches
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Nonspecific phospholipases C3 and C4 interact with PIN-FORMED2 to regulate growth and tropic responses in Arabidopsis Plant Cell (IF 11.6) Pub Date : 2024-03-05 Teng Li, Shujuan Zhang, Shuaibing Yao, Xuebing Li, Qianru Jia, Jingya Yuan, Wenhua Zhang, Xuemin Wang, Qun Zhang
The dynamic changes in membrane phospholipids affect membrane biophysical properties and cell signaling, thereby influencing numerous biological processes. Nonspecific phospholipase C (NPC) enzymes hydrolyze common phospholipids to release diacylglycerol (DAG), which is converted to phosphatidic acid (PA) and other lipids. In this study, two Arabidopsis (Arabidopsis thaliana) tandemly arrayed genes
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OPEN STOMATA1 phosphorylates CYCLIC NUCLEOTIDE-GATED CHANNELs to trigger Ca2+ signaling for ABA-induced stomatal closure in Arabidopsis Plant Cell (IF 11.6) Pub Date : 2024-03-05 Yang Yang, Yan-Qiu Tan, Xinyong Wang, Jia-Jun Li, Bo-Ya Du, Meijun Zhu, Pengcheng Wang, Yong-Fei Wang
Multiple cyclic nucleotide-gated channels (CNGCs) are abscisic acid (ABA)-activated Ca2+ channels in Arabidopsis (Arabidopsis thaliana) guard cells. In particular, CNGC5, CNGC6, CNGC9, and CNGC12 are essential for ABA-specific cytosolic Ca2+ signaling and stomatal movements. However, the mechanisms underlying ABA-mediated regulation of CNGCs and Ca2+ signaling are still unknown. In this study, we identified
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Identification and functional characterization of conserved cis-regulatory elements responsible for early fruit development in cucurbit crops Plant Cell (IF 11.6) Pub Date : 2024-02-29 Hongjia Xin, Xin Liu, Sen Chai, Xueyong Yang, Hongbo Li, Bowen Wang, Yuanchao Xu, Shengnan Lin, Xiaoyun Zhong, Bin Liu, Zefu Lu, Zhonghua Zhang
A number of cis-regulatory elements (CREs) conserved during evolution have been found to be responsible for phenotypic novelty and variation. Cucurbit crops such as cucumber (Cucumis sativus), watermelon (Citrullus lanatus), melon (Cucumis melo) and squash (Cucurbita maxima) develop fruits from an inferior ovary and share some similar biological processes during fruit development. Whether conserved
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Brassinosteroid-dependent phosphorylation of PHOSPHATE STARVATION RESPONSE2 reduces its DNA-binding ability in rice Plant Cell (IF 11.6) Pub Date : 2024-02-28 Guoxia Zhang, Hongru Wang, Xiangle Ren, Yunhua Xiao, Dapu Liu, Wenjing Meng, Yahong Qiu, Bin Hu, Qingjun Xie, Chengcai Chu, Hongning Tong
Brassinosteroids (BRs) are widely used as plant growth regulators in modern agriculture. Understanding how BRs regulate nutrient signaling is crucial for reducing fertilizer usage. Here we elucidate that the central BR signaling inhibitor GSK3/SHAGGY-LIKE KINASE2 (GSK2) interacts directly with and phosphorylates PHOSPHATE STARVATION RESPONSE2 (OsPHR2), the key regulator of phosphate (Pi) signaling
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Integrative omics analysis elucidates the genetic basis underlying seed weight and oil content in soybean Plant Cell (IF 11.6) Pub Date : 2024-02-27 Xiaobo Yuan, Xinyu Jiang, Mengzhu Zhang, Longfei Wang, Wu Jiao, Huatao Chen, Junrong Mao, Wenxue Ye, Qingxin Song
Synergistic optimization of key agronomic traits by traditional breeding has dramatically enhanced crop productivity in the past decades. However, the genetic basis underlying coordinated regulation of yield- and quality-related traits remains poorly understood. Here, we dissected the genetic architectures of seed weight and oil content by combining genome-wide association studies (GWAS) and transcriptome-wide
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Arabidopsis as a model for translational research Plant Cell (IF 11.6) Pub Date : 2024-02-27 Anna E Yaschenko, Jose M Alonso, Anna N Stepanova
Arabidopsis thaliana is currently the most-studied plant species on earth, with an unprecedented number of genetic, genomic, and molecular resources having been generated in this plant model. In the era of translating foundational discoveries to crops and beyond, we aimed to highlight the utility and challenges of using Arabidopsis as a reference for applied plant biology research, agricultural innovation
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The landscape of transcription factor promoter activity during vegetative development in Marchantia Plant Cell (IF 11.6) Pub Date : 2024-02-23 Facundo Romani, Susanna Sauret-Güeto, Marius Rebmann, Davide Annese, Ignacy Bonter, Marta Tomaselli, Tom Dierschke, Mihails Delmans, Eftychios Frangedakis, Linda Silvestri, Jenna Rever, John L Bowman, Ignacio Romani, Jim Haseloff
Transcription factors (TFs) are essential for the regulation of gene expression and cell fate determination. Characterising the transcriptional activity of TF genes in space and time is a critical step towards understanding complex biological systems. The vegetative gametophyte meristems of bryophytes share some characteristics with the shoot apical meristems of flowering plants. However, the identity
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An auxin research odyssey: 1989-2023 Plant Cell (IF 11.6) Pub Date : 2024-02-21 Jerry D Cohen, Lucia C Strader
The phytohormone auxin is at times called the master regulator of plant processes and has been shown to be a central player in embryo development, the establishment of the polar axis, early aspects of seedling growth, as well as growth and organ formation during later stages of plant development. The Plant Cell has been key, since the inception of the journal, to developing an understanding of auxin
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Genome organization and botanical diversity Plant Cell (IF 11.6) Pub Date : 2024-02-21 Andrew H Paterson, Christine Queitsch
The rich diversity of angiosperms, both the planet’s dominant flora and the cornerstone of agriculture, is integrally intertwined with a distinctive evolutionary history. Here, we explore the interplay between angiosperm genome organization and botanical diversity, empowered by genomic approaches ranging from genetic mapping to analysis of gene regulation. Commonality in the genetic hardware of plants
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Not so hidden anymore: Advances and challenges in understanding root growth under water deficits Plant Cell (IF 11.6) Pub Date : 2024-02-21 Priya Voothuluru, Yajun Wu, Robert E Sharp
Limited water availability is a major environmental factor constraining plant development and crop yields. One of the prominent adaptations of plants to water deficits is the maintenance of root growth that enables sustained access to soil water. Despite early recognition of the adaptive significance of root growth maintenance under water deficits, progress in understanding has been hampered by the
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CO-EXPRESSED WITH PSI ASSEMBLY1 (CEPA1) is a photosystem I assembly factor in Arabidopsis Plant Cell (IF 11.6) Pub Date : 2024-02-21 David Rolo, Omar Sandoval-Ibáñez, Wolfram Thiele, Mark A Schöttler, Ines Gerlach, Reimo Zoschke, Joram Schwartzmann, Etienne H Meyer, Ralph Bock
Photosystem I (PSI) forms a large macromolecular complex of ∼580 kDa that resides in the thylakoid membrane and mediates photosynthetic electron transfer. PSI is composed of eighteen protein subunits and nearly two hundred co-factors. The assembly of the complex in thylakoid membranes requires high spatial and temporal coordination, and is critically dependent on a sophisticated assembly machinery
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Molecular dissection of an intronic enhancer governing cold-induced expression of the vacuolar invertase gene in potato Plant Cell (IF 11.6) Pub Date : 2024-02-20 Xiaobiao Zhu, Airu Chen, Nathaniel M Butler, Zixian Zeng, Haoyang Xin, Lixia Wang, Zhaoyan Lv, Dani Eshel, David S Douches, Jiming Jiang
Potato (Solanum tuberosum) is the third most important food crop in the world. Potato tubers must be stored at cold temperatures to minimize sprouting and losses due to disease. However, cold temperatures strongly induce the expression of the potato vacuolar invertase gene (VInv) and cause reducing sugar accumulation. This process, referred to as “cold-induced sweetening”, is a major postharvest problem
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Maternal nitric oxide homeostasis impacts female gametophyte development under optimal and stress conditions Plant Cell (IF 11.6) Pub Date : 2024-02-20 Junzhe Wang, Xiaolong Guo, Yijin Chen, Tianxiang Liu, Jianchu Zhu, Shengbao Xu, Elizabeth Vierling
In adverse environments, the number of fertilizable female gametophytes (FGs) in plants is reduced, leading to increased survival of remaining offspring. How the maternal plant perceives internal growth cues and external stress conditions to alter FG development remains largely unknown. We report that homeostasis of the stress signaling molecule nitric oxide (NO) plays a key role in controlling FG
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The chromatin remodeler ERCC6 and the histone chaperone NAP1 are involved in apurinic/apyrimidinic endonuclease-mediated DNA repair Plant Cell (IF 11.6) Pub Date : 2024-02-17 Tianyi Fan, Tianfang Shi, Ran Sui, Jingqi Wang, Huijia Kang, Yao Yu, Yan Zhu
During base excision repair (BER), the apurinic or apyrimidinic (AP) site serves as an intermediate product following base excision. In plants, APE-redox protein (ARP) represents the major AP site of cleavage activity. Despite the well-established understanding that the nucleosomal structure acts as a barrier to various DNA-templated processes, the regulatory mechanisms underlying BER at the chromatin
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MAC3A and MAC3B mediate degradation of the transcription factor ERF13 and thus promote lateral root emergence Plant Cell (IF 11.6) Pub Date : 2024-02-17 Zipeng Yu, Xingzhen Qu, Bingsheng Lv, Xiaoxuan Li, Jiaxuan Sui, Qianqian Yu, Zhaojun Ding
Lateral roots (LRs) increase root surface area and allow plants greater access to soil water and nutrients. LR formation is tightly regulated by the phytohormone auxin. Whereas the transcription factor ETHYLENE-RESPONSIVE ELEMENT BINDING FACTOR13 (ERF13) prevents LR emergence in Arabidopsis (Arabidopsis thaliana), auxin activates MITOGEN-ACTIVATED PROTEIN KINASE14 (MPK14), which leads to ERF13 degradation
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Reflections on the ABC model of flower development Plant Cell (IF 11.6) Pub Date : 2024-02-12 John L Bowman, Edwige Moyroud
The formulation of the ABC model by a handful of pioneer plant developmental geneticists was a seminal event in the quest to answer a seemingly simple question: how are flowers formed? Fast forward 30 years and this elegant model has generated a vibrant and diverse community, capturing the imagination of developmental and evolutionary biologists, structuralists, biochemists and molecular biologists
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Pan-transcriptomic analysis reveals alternative splicing control of cold tolerance in rice Plant Cell (IF 11.6) Pub Date : 2024-02-12 Yuanyuan Zhong, Yuhong Luo, Jinliang Sun, Xuemei Qin, Ping Gan, Zuwen Zhou, Yongqing Qian, Rupeng Zhao, Zhiyuan Zhao, Wenguo Cai, Jijing Luo, Ling-Ling Chen, Jia-Ming Song
Plants have evolved complex mechanisms to adapt to harsh environmental conditions. Rice (Oryza sativa) is a staple food crop that is sensitive to low temperatures. However, its cold stress responses remain poorly understood, thus limiting possibilities for crop engineering to achieve greater cold tolerance. In this study, we constructed a rice pan-transcriptome and characterized its transcriptional
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The Myb73-GDPD2-GA2ox1 transcriptional regulatory module confers phosphate deficiency tolerance in soybean Plant Cell (IF 11.6) Pub Date : 2024-02-12 Dandan Hu, Ruifan Cui, Ke Wang, Yuming Yang, Ruiyang Wang, Hongqing Zhu, Mengshi He, Yukun Fan, Le Wang, Li Wang, Shanshan Chu, Jinyu Zhang, Shanshan Zhang, Yifei Yang, Xuhao Zhai, Haiyan Lv, Dandan Zhang, Jinshe Wang, Fanjiang Kong, Deyue Yu, Hengyou Zhang, Dan Zhang
Phosphorus is indispensable in agricultural production. An increasing food supply requires more efficient use of phosphate due to limited phosphate resources. However, how crops regulate phosphate efficiency remains largely unknown. Here, we identified a major quantitative trait locus, qPE19, that controls seven low-phosphate (LP)-related traits in soybean (Glycine max) through linkage mapping and
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Transcription factor PagMYB31 positively regulates cambium activity and negatively regulates xylem development in poplar Plant Cell (IF 11.6) Pub Date : 2024-02-10 Yanhui Zhang, Song Chen, Linghua Xu, Shimin Chu, Xiaojing Yan, Lanying Lin, Jialong Wen, Bo Zheng, Su Chen, Quanzi Li
Wood formation involves consecutive developmental steps, including cell division of vascular cambium, xylem cell expansion, secondary cell wall (SCW) deposition, and programmed cell death. In this study, we identified PagMYB31 as a coordinator regulating these processes in Populus alba × Populus glandulosa and built a PagMYB31-mediated transcriptional regulatory network. PagMYB31 mutation caused fewer
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GRAS transcription factor PINNATE-LIKE PENTAFOLIATA2 controls compound leaf morphogenesis in Medicago truncatula Plant Cell (IF 11.6) Pub Date : 2024-02-06 Liangliang He, Ye Liu, Yawen Mao, Xinyuan Wu, Xiaoling Zheng, Weiyue Zhao, Xiaoyu Mo, Ruoruo Wang, Qinq Wu, Dongfa Wang, Youhan Li, Yuanfan Yang, Quanzi Bai, Xiaojia Zhang, Shaoli Zhou, Baolin Zhao, Changning Liu, Yu Liu, Million Tadege, Jianghua Chen
The milestone of compound leaf development is the generation of separate leaflet primordia during the early stages, which involves two linked but distinct morphogenetic events: leaflet initiation and boundary establishment for leaflet separation. Although some progress in understanding the regulatory pathways for each event have been made, it is unclear how they are intrinsically coordinated. Here