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Blockade of sodium‑calcium exchanger via ORM-10962 attenuates cardiac alternans J. Mol. Cell. Cardiol. (IF 4.133) Pub Date : 2020-12-28 Jozefina Szlovák; Jakub Tomek; Xin Zhou; Noémi Tóth; Roland Veress; Balázs Horváth; Norbert Szentandrássy; Jouko Levijoki; Julius Gy. Papp; Neil Herring; András Varró; David A. Eisner; Blanca Rodriguez; Norbert Nagy
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Adverse effects of hydroxychloroquine and azithromycin on contractility and arrhythmogenicity revealed by human engineered cardiac tissues J. Mol. Cell. Cardiol. (IF 4.133) Pub Date : 2020-12-27 Andy On-Tik Wong; Bimal Gurung; Wing Sum Wong; Suet Yee Mak; Wan Wai Tse; Chloe M. Li; Deborah K. Lieu; Kevin D. Costa; Ronald A. Li; Roger J. Hajjar
The coronavirus disease 2019 (COVID-19) outbreak caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has become a global pandemic as declared by World Health Organization (WHO). In the absence of an effective treatment, different drugs with unknown effectiveness, including antimalarial hydroxychloroquine (HCQ), with or without concurrent administration with azithromycin (AZM), have
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Secalin films acylated with capric acid chloride Food Biosci. (IF 3.067) Pub Date : 2021-01-12 Zeinab Qazanfarzadeh; Mahdi Kadivar; Hajar Shekarchizadeh; Raffaele Porta
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Polyacetylene (9Z,16S)-16-hydroxy-9,17-octadecadiene-12,14-diynoic acid in Dendropanax morbifera leaves Food Biosci. (IF 3.067) Pub Date : 2021-01-07 Mun-Ock Kim; Myung-Ji Kang; Su-Ui Lee; Doo-Young Kim; Hyun-Jae Jang; Ju Hyeon An; Hyun-Sun Lee; Hyung Won Ryu; Sei-Ryang Oh
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Zinc supplementation modulates intracellular metal uptake and oxidative stress defense mechanisms in CHO cell cultures Biochem. Eng. J. (IF 3.475) Pub Date : 2021-01-15 Ryan J. Graham; Stephanie A. Ketcham; Adil Mohammad; Evan Paregol; Seongkyu Yoon; Guozhang Zou; Tongzhong Ju; Patrick J. Faustino; Muhammad Ashraf; Chikkathur N. Madhavarao
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Bubble barriers to guide downstream migrating Atlantic salmon (Salmo salar): An evaluation using acoustic telemetry Ecol. Eng. (IF 3.512) Pub Date : 2021-01-14 J. Leander; J. Klaminder; G. Hellström; M. Jonsson
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Semi-continuous production of xanthan in biofilm reactor using Xanthomonas campestris J. Biotechnol. (IF 3.503) Pub Date : 2021-01-13 Maryam Nejadmansouri; Maryam Razmjooei; Reza Safdarianghomsheh; Ehsan Shad; Frank Delvigne; Mohammadreza Khalesi
Semi-continuous production of xanthan gum using self-immobilized Xanthomonas campestris cells in biofilm reactors was studied. Fermentation was carried out using two different designs of biofilm reactor equipped with a) stainless-steel support (SSS) and b) polyethylene support (PES). Fermentation was performed in three cycles with refreshing the media at the beginning of each: cycle 1, 0−27 h; cycle
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The relationship between the hand pattern used during fast wheelchair propulsion and shoulder pain development J. Biomech. (IF 2.32) Pub Date : 2020-12-28 Shelby L. Walford; Jeffery W. Rankin; Sara J. Mulroy; Richard R. Neptune
Up to 84% of manual wheelchair users (MWCU) with spinal cord injury experience shoulder pain, which is correlated with shoulder adductor weakness in this population. Modeling studies have shown weak shoulder adductors lead to compensations from the deltoid and rotator cuff muscles during propulsion, which may lead to altered propulsion mechanics. However, the role recovery phase hand pattern has in
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A non–invasive technique for evaluating carpal tunnel pressure with ultrasound vibro–elastography for patients with carpal tunnel syndrome: A pilot clinical study J. Biomech. (IF 2.32) Pub Date : 2021-01-07 Yoichi Toyoshima; Boran Zhou; Kazutoshi Kubo; Kai-Nan An; Steven L. Moran; Xiaoming Zhang; Peter C. Amadio; Chunfeng Zhao
Carpal tunnel syndrome (CTS) is a disorder that affects the median nerve at the wrist sufficient to cause impairment of nerve function. Elevated carpal tunnel pressure (CTP) leads to median nerve pathology, sensory, and motor changes in CTS patient. The techniques to quantify CTP used in clinic are invasive. This study aimed to investigate the feasibility of a noninvasive ultrasound vibro–elastography
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Increased force following muscle stretching and simultaneous fibre shortening: Residual force enhancement or force depression – That is the question? J. Biomech. (IF 2.32) Pub Date : 2021-01-02 Sheharzad Mahmood; Andrew Sawatsky; Walter Herzog
Residual force enhancement (rFE) describes the increase in isometric force following muscle stretching compared to the corresponding isometric force. Even though rFE is consistently observed in isolated muscle preparations, it is not always observed in human skeletal muscle. This inconsistency might be associated with disociations between length changes in muscle tendon units (MTUs) and fibres. This
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Relationships between tibial acceleration and ground reaction force measures in the medial-lateral and anterior-posterior planes J. Biomech. (IF 2.32) Pub Date : 2021-01-15 Caleb D. Johnson; Jereme Outerleys; Irene S. Davis
Peak vertical tibial accelerations during running have shown strong correlations with vertical ground reaction force loading rates and some associations with injury. However, little attention has been given to tibial accelerations along the medial-lateral and anterior-posterior axes. Therefore, our purpose was to examine the correlation between peak tibial accelerations and ground reaction force loading
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Video-based biomechanical analysis of an unexpected Achilles tendon rupture in an Olympic sprinter J. Biomech. (IF 2.32) Pub Date : 2021-01-15 Irineu Loturco; Carlos De la Fuente; Chris Bishop; Hélio Nichioka; Denise Lessio; Carlos T. Moreno; Lucas A. Pereira; Felipe P. Carpes
We used image-processing techniques to determine the moment (i.e., image frame) of the Achilles tendon (AT) rupture in an Olympic sprinter. This report may be unique due to the difficulty in conducting motion capture analyses during injury events. Our report includes one female Olympic sprinter, 29 years old (body mass: 56 kg, height: 1.68 m, and body mass index: 19.8 kg/m2) with a high-competitive
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Modeling muscle function using experimentally determined subject-specific muscle properties J. Biomech. (IF 2.32) Pub Date : 2021-01-15 J.M. Wakeling; C. Tijs; N. Konow; A.A. Biewener
Muscle models are commonly based on intrinsic properties pooled across a number of individuals, often from a different species, and rarely validated against directly measured muscle forces. Here we use a rich data set of rat medial gastrocnemius muscle forces recorded during in-situ and in-vivo isometric, isotonic, and cyclic contractions to test the accuracy of forces predicted using Hill-type muscle
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Computational and experimental analysis of a Glaucoma flat drainage device J. Biomech. (IF 2.32) Pub Date : 2021-01-15 R.M.R. Panduro; Christian Monterrey; J.L. Mantari; Ruth Canahuire; H. Alvarez; Mario Miranda; Ahmed Elsheikh
This paper presents a computational and experimental analysis of a glaucoma flat drainage device (FDD). The FDD consists of a metallic microplate placed into the eye sclerocorneal limbus, which creates a virtual path between the anterior chamber and its exterior, allowing the intraocular pressure (IOP) to be kept in a normal range. It also uses the surrounding tissue as a flow regulator in order to
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The gait is less stable in children with cerebral palsy in normal and dual-task gait compared to typically developed peers J. Biomech. (IF 2.32) Pub Date : 2021-01-15 Harri Piitulainen; Juha-Pekka Kulmala; Helena Mäenpää; Timo Rantalainen
There is limited evidence about gait stability and its alteration by concurrent motor and cognitive tasks in children with cerebral palsy (CP). We examined gait stability and how it is altered by constrained cognitive or motor task in CP and their typically developed (TD) controls. Gait kinematics were recorded using inertial-measurement units (IMU) from 18 patients with hemiplegia (13.5±2.4 years)
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Differentiation of fluctuations in uterine contractions associated with Term pregnancies using adaptive fractal features of electromyography signals Med. Eng. Phys. (IF 1.737) Pub Date : 2021-01-05 P. Vardhini; N. Punitha; S. Ramakrishnan
Analysis of uterine contractions using electromyography signals is gaining importance due to its capability to measure the dynamics of uterus. Uterine electromyography (uEMG) provides information on the nature of uterine contractions non-invasively. In this study, the fluctuations in uEMG signals associated with Term pregnancies are analyzed. For this, Term uEMG signals corresponding to second (T1)
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Structures, Chemical Conversions, and Cytotoxicity of Tricholopardins C and D, Two Tricholoma Triterpenoids from the Wild Mushroom Tricholoma pardinum Nat. Prod. Bioprospect. Pub Date : 2021-01-15 Chen Shi, Yue-Ling Peng, Juan He, Zheng-Hui Li, Ji-Kai Liu, Tao Feng
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A Protein‐free Chemically Defined Medium for the Cultivation of Various Microorganisms with Food Safety Significance J. Appl. Microbiol. (IF 3.066) Pub Date : 2021-01-15 Danhui Wang; Peyton Greenwood; Matthias S. Klein
To develop a broadly applicable medium free of proteins with well‐defined and reproducible chemical composition for the cultivation of various microorganisms with food safety significance.
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Survival of Clostridioides difficile in finished dairy compost under controlled conditions J. Appl. Microbiol. (IF 3.066) Pub Date : 2021-01-15 Muthu Dharmasena; Hongye Wang; Tianhui Wei; William C. Bridges; Xiuping Jiang
The survival of Clostridioides difficile (previously Clostridium difficile) vegetative cells and endospores was compared at different levels of indigenous microflora using autoclaved and unautoclaved dairy composts with different moisture contents (MCs).
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Different effects of probiotics and antibiotics on the composition of microbiota, SCFAs concentrations and FFAR2/3 mRNA expression in broiler chickens J. Appl. Microbiol. (IF 3.066) Pub Date : 2020-12-01 J.‐M. Zhang; X.‐Y. Liu; W. Gu; H.‐Y. Xu; H.‐C. Jiao; J.‐P. Zhao; X.‐J. Wang; H.‐F. Li; H. Lin
The aims of this study were to investigate the effects of probiotics and antibiotics on microbial composition, short chain fatty acids (SCFAs) concentration and free fatty acid receptor 2/3 (FFAR2/3) expression in boiler chickens.
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Balancing glucose and oxygen uptake rates to enable high amorpha‐4,11‐diene production in Escherichia coli via the methylerythritol phosphate pathway Biotechnol. Bioeng. (IF 4.002) Pub Date : 2020-12-17 Vikas Patil; Christine N. S. Santos; Parayil K. Ajikumar; Stephen Sarria; Ralf Takors
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Temperature regulation as a tool to program synthetic microbial community composition Biotechnol. Bioeng. (IF 4.002) Pub Date : 2021-01-05 Adam G. Krieger; Jiahao Zhang; Xiaoxia N. Lin
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Asiaticoside inhibits renal fibrosis development by regulating the miR‐142‐5p/ACTN4 axis Biotechnol. Appl. Bioc. (IF 1.638) Pub Date : 2021-01-14 Min Zhang; Shuhuan Liu; Liming Fang; Gang Wang
Renal fibrosis results in the progressive renal dysfunction and leads to chronic kidney disease (CKD) and ultimately end‐stage renal disease. Asiaticoside was reported to regulate synaptopodin, desmin, nephrin and podocin levels in adriamycin‐induced nephropathy of rats. In this study, we found out that asiaticoside inhibited renal fibrosis in vitro and in vivo. Additionally, miR‐142‐5p was upregulated
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In vitro hepatic steatosis model based on gut–liver‐on‐a‐chip Biotechnol. Prog. (IF 2.334) Pub Date : 2021-01-03 Joong‐won Jeon; Seung Hwan Lee; Donghyun Kim; Jong Hwan Sung
Hepatic steatosis, also known as fatty liver disease, occurs due to abnormal lipid accumulation in the liver. It has been known that gut absorption also plays an important role in the mechanism underlying hepatic steatosis. Conventional in vitro cell culture models have limitations in recapitulating the mechanisms of hepatic steatosis because it does not include the gut absorption process. Previously
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Engineered Aptamers for Enhanced COVID-19 Theranostics Cel. Mol. Bioeng. (IF 1.72) Pub Date : 2021-01-15 Caleb Acquah, Jaison Jeevanandam, Kei Xian Tan, Michael K. Danquah
Introduction The 2019-novel coronavirus disease (COVID-19) is an intractable global health challenge resulting in an aberrant rate of morbidity and mortality worldwide. The mode of entry for SARS-CoV-2 into host cells occurs through clathrin-mediated endocytosis. As part of the efforts to mitigate COVID-19 infections, rapid and accurate detection methods, as well as smart vaccine and drug designs with
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In Vitro Assessment of Right Ventricular Outflow Tract Anatomy and Valve Orientation Effects on Bioprosthetic Pulmonary Valve Hemodynamics Cardiovasc. Eng. Technol. (IF 1.771) Pub Date : 2021-01-15 Nicole K. Schiavone, Christopher J. Elkins, Doff B. McElhinney, John K. Eaton, Alison L. Marsden
Purpose The congenital heart defect Tetralogy of Fallot (ToF) affects 1 in 2500 newborns annually in the US and typically requires surgical repair of the right ventricular outflow tract (RVOT) early in life, with variations in surgical technique leading to large disparities in RVOT anatomy among patients. Subsequently, often in adolescence or early adulthood, patients usually require surgical placement
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Image‐Based Computational Fluid Dynamics for Estimating Pressure Drop and FFR Across Iliac Artery Stenosis: A Comparison with in‐Vivo Measurements Int. J. Numer. Method. Biomed. Eng. (IF 2.097) Pub Date : 2021-01-15 S. Skopalik; P. Hall Barrientos; J. Matthews; A. Radjenovic; P. Mark; G. Roditi; M. C. Paul
Computational Fluid Dynamics (CFD) and time‐resolved phase‐contrast magnetic resonance imaging (PC‐MRI) are potential non‐invasive methods for the assessment of the severity of arterial stenoses. Fractional flow reserve (FFR) is the current “gold standard” for determining stenosis severity in the coronary arteries but is an invasive method requiring insertion of a pressure wire. CFD derived FFR (vFFR)
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Regulation of cuticular wax biosynthesis in plants under abiotic stress Plant Biotechnol. Rep. (IF 1.462) Pub Date : 2021-01-15 Md Shaheenuzzamn, Shandang Shi, Kamran Sohail, Hongqi Wu, Tianxiang Liu, Peipei An, Zhonghua Wang, Mirza Hasanuzzaman
Cuticular waxes are the covering of the outer layer of the plant, consist of hydrocarbon appears like whitish film or bloom in plant organs. They play a vital role like a safeguard from different stress condition in the plant. Since environmental factors are active regulators of cuticular wax biosynthesis, composition, quantity, and deposition, it is evident that cuticular wax is associated with plant
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MicroRNA-146a-5p enhances T helper 17 cell differentiation via decreasing a disintegrin and metalloprotease 17 level in primary sjögren’s syndrome Bioengineered (IF 2.205) Pub Date : 2021-01-15 Xiaoyan Wang; Shaojun Xin; Youqing Wang; Duo Ju; Quan Wu; Ye Qiu; Xuemin Niu; Wei Liu; Jianyou Li; Pengtian Ji
ABSTRACT In clinical practice, we found that microRNA (miR)-146a-5p is significantly up-regulated in peripheral blood mononuclear cells (PBMCs) of primary sjögren’s syndrome (pSS) patients. In vitro experiments confirmed that miR-146a-5p promotes T helper 17 (Th17) cell differentiation, but the specific mechanism is still unknown. To solve this problem, 20 pSS patients and 20 healthy subjects were
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Production of L-carnitine-enriched edible filamentous fungal biomass through submerged cultivation Bioengineered (IF 2.205) Pub Date : 2021-01-15 Neda Rousta; Jorge A. Ferreira; Mohammad J. Taherzadeh
ABSTRACT The edible filamentous fungi are hot candidate for future supply of functional food and feed with e.g. protein, essential amino acids, and compounds with immunostimulant activity. L-carnitine that plays a crucial role in energy metabolism represents a functional compound normally produced by Zygomycetes filamentous fungus Rhizopus oligosporus in solid-state fermentation. The present study
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Regulatory effects of transition metals supplementation/deficiency on the gut microbiota Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-01-15 Cheng-Yu Li, Xin-Yu Li, Liang Shen, Hong-Fang Ji
Abstract Transition metal ions are essential micronutrients for all living organisms and exert a wide range of effects on human health. The uptake of transition metal ions occurs primarily in the gastrointestinal tract, which is colonized by trillions of bacterial cells. In recent years, increasing studies have indicated that transition metals have regulatory effects on the gut microbiota. In view
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Hydrolytic secretome engineering in Yarrowia lipolytica for consolidated bioprocessing on polysaccharide resources: review on starch, cellulose, xylan, and inulin Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-01-15 Ewelina Celińska, Jean-Marc Nicaud, Wojciech Białas
Abstract Consolidated bioprocessing (CBP) featuring concomitant hydrolysis of renewable substrates and microbial conversion into value-added biomolecules is considered to bring substantial benefits to the overall process efficiency. The biggest challenge in developing an economically feasible CBP process is identification of bifunctional biocatalyst merging the ability to utilize the substrate and
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Transformed tissue of Dionaea muscipula J. Ellis as a source of biologically active phenolic compounds with bactericidal properties Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-01-15 Wojciech Makowski, Aleksandra Królicka, Anna Nowicka, Jana Zwyrtková, Barbara Tokarz, Ales Pecinka, Rafał Banasiuk, Krzysztof Michał Tokarz
Abstract The Venus flytrap (Dionaea muscipula J. Ellis) is a carnivorous plant able to synthesize large amounts of phenolic compounds, such as phenylpropanoids, flavonoids, phenolic acids, and 1,4-naphtoquinones. In this study, the first genetic transformation of D. muscipula tissues is presented. Two wild-type Rhizobium rhizogenes strains (LBA 9402 and ATCC 15834) were suitable vector organisms in
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Xeno-Free Bioreactor Culture of Human Mesenchymal Stromal Cells on Chemically Defined Microcarriers ACS Biomater. Sci. Eng. (IF 4.152) Pub Date : 2021-01-14 John D. Krutty; Kevin Koesser; Stephen Schwartz; Junsu Yun; William L. Murphy; Padma Gopalan
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Single-Cell Quantification of the Transition Temperature of Intracellular Elastin-like Polypeptides ACS Biomater. Sci. Eng. (IF 4.152) Pub Date : 2021-01-14 David R. Tyrpak; Yaocun Li; Siqi Lei; Hugo Avila; John Andrew MacKay
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Two Magnetic Sensor Based Real-Time Tracking of Magnetically Inflated Swallowable Intragastric Balloon Ann. Biomed. Eng. (IF 3.324) Pub Date : 2021-01-15 Muneaki Miyasaka, Anthony Meng Huat Tiong, Phuoc Thien Phan, Yanpei Huang, Hung Leng Kaan, Khek Yu Ho, Soo Jay Phee
This paper presents a two magnetic sensor based tracking method for a magnetically inflated intragastric balloon capsule (MIBC) which is used for obesity treatment. After the MIBC is swallowed, it is designed to be inflated inside the stomach by approaching a permanent magnet (PM) externally near the abdomen. However, if the balloon inflation is accidentally triggered while the MIBC is still in the
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Letter to the Editor Biotech. Histochem. (IF 1.203) Pub Date : 2020-11-06 Armen Nersesyan
(2021). Letter to the Editor. Biotechnic & Histochemistry: Vol. 96, No. 2, pp. 83-84.
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Comparison of two-dimensional synthesized mammograms versus original digital mammograms: a quantitative assessment Med. Biol. Eng. Comput. (IF 2.022) Pub Date : 2021-01-14 Maxine Tan, Mundher Al-Shabi, Wai Yee Chan, Leya Thomas, Kartini Rahmat, Kwan Hoong Ng
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Phospholipase A2 enzyme from the venom of Egyptian honey bee Apis mellifera lamarckii with anti-platelet aggregation and anti-coagulation activities J. Genet. Eng. Biotechnol. Pub Date : 2021-01-14 Doaa A. Darwish; Hassan M. M. Masoud; Mohamed M. Abdel-Monsef; Mohamed S. Helmy; Hind A. Zidan; Mahmoud A. Ibrahim
Honey bee venom contains various enzymes with wide medical and pharmaceutical applications. The phospholipase A2 (PLA2) has been apparently purified from the venom of Egyptian honey bee (Apis mellifera lamarckii) 8.9-fold to a very high specific activity of 6033 U/mg protein using DEAE–cellulose and Sephacryl S-300 columns. The purified bee venom PLA2 is monomeric 16 kDa protein and has isoelectric
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Biogenic synthesis of gold nanoparticles using Argemone mexicana L. and their cytotoxic and genotoxic effects on human colon cancer cell line (HCT-15) J. Genet. Eng. Biotechnol. Pub Date : 2021-01-14 Kailas D. Datkhile; Satish R. Patil; Pratik P. Durgawale; Madhavi N. Patil; Dilip D. Hinge; Nilam J. Jagdale; Vinit N. Deshmukh; Ashwini L. More
Nanomedicine has evolved as precision medicine in novel therapeutic approach of cancer management. The present study investigated the efficacy of biogenic gold nanoparticles synthesized using Argemone mexicana L. aqueous extract (AM-AuNPs) against the human colon cancer cell line, HCT-15. Biosynthesis of AM-AuNPs was determined by ultraviolet-visible spectroscopy and further characterized by transmission
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Novel Modalities in DNA Data Storage Trends Biotechnol. (IF 14.343) Pub Date : 2021-01-14 Cheng Kai Lim; Saurabh Nirantar; Wen Shan Yew; Chueh Loo Poh
The field of storing information in DNA has expanded exponentially. Most common modalities involve encoding information from bits into synthesized nucleotides, storage in liquid or dry media, and decoding via sequencing. However, limitations to this paradigm include the cost of DNA synthesis and sequencing, along with low throughput. Further unresolved questions include the appropriate media of storage
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Comparative infectivity and biocontrol potential of Acrobeloides k29 and entomopathogenic nematodes on the leopard moth borer, Zeuzera pyrina Biol. Control (IF 2.754) Pub Date : 2021-01-04 Elham Salari; Javad Karimi; Majid Fasihi Harandi; Hussein Sadeghi Nameghi
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Metabolic engineering of Thermoanaerobacterium aotearoense SCUT27 for glucose and cellobiose co-utilization by identification and overexpression of the endogenous cellobiose operon Biochem. Eng. J. (IF 3.475) Pub Date : 2021-01-07 Chunyun Qu; Yang Zhang; Kaiqun Dai; Hongxin Fu; Jufang Wang
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A novel smartphone-based colorimetric biosensor for reliable quantification of hydrogen peroxide by enzyme-inorganic hybrid nanoflowers Biochem. Eng. J. (IF 3.475) Pub Date : 2021-01-07 Miaorong Zhang; Yan Zhang; Chuankai Yang; Chunyun Ma; Jianguo Tang
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Fed-batch culture of Oplopanax elatus adventitious roots: Feeding medium selection through comprehensive evaluation using an analytic hierarchy process Biochem. Eng. J. (IF 3.475) Pub Date : 2021-01-08 X.L. Jiang; M.Y. Jin; X.C. Piao; C.R. Yin; M.L. Lian
Adventitious root (AR) culture is an efficient route for the production of bioactive compounds of Oplopanax elatus. In fed-batch culture, selecting feeding media is a critical strategy for the successful culture. Therefore, this study examined the effects of four feeding medium factors (sucrose concentration, medium salt strength, phosphorus and nitrogen concentration) on culture efficiency of O. elatus
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The Detection of Vancomycin in Sweat: A Next-Generation Digital Surrogate Marker for Antibiotic Tissue Penetration: A Pilot Study Digit Biomark. Pub Date : 2021-01-14 Noé Brasier; Andreas Widmer; Michael Osthoff; Markus Mutke; Fiorangelo De Ieso; Pascale Brasier-Lutz; Kitty Brown; Linxing Yao; Corey D. Broeckling; Jessica Prenni; Jens Eckstein
Background: Assuring adequate antibiotic tissue concentrations at the point of infection, especially in skin and soft tissue infections, is pivotal for an effective treatment and cure. Despite the global issue, a reliable AB monitoring test is missing. Inadequate antibiotic treatment leads to the development of antimicrobial resistances and toxic side effects. β-lactam antibiotics were already detected
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The commercial tree species Dipteryx odorata improves soil physical and biological attributes in abandoned pastures Ecol. Eng. (IF 3.512) Pub Date : 2021-01-14 Alberto Bentes Brasil Neto; Nilza Martins de Queiroz Xavier Brasil; Paulo Ivan Lima de Andrade; Augusto César Falcão Sampaio; Norberto Cornejo Noronha; Eduardo Jorge Maklouf Carvalho; Arystides Resende Silva; Gustavo Schwartz
In the Eastern Amazon, abandoned and unproductive areas are widespread and normally found as a result of soil degradation. In this study we presented the following scientific question: Is it possible to improve the quality of soil physical and biological attributes of areas previously occupied by abandoned pastures with commercial plantation of the native species Dipteryx odorata? To respond this question
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Improving bypass performance and passage success of Atlantic salmon smolts at an old fish-hostile hydroelectric power station: a challenging task Ecol. Eng. (IF 3.512) Pub Date : 2021-01-13 Michaël Ovidio; Séverine Renardy; Arnaud Dierckx; Billy Nzau Matondo; Jean-Philippe Benitez
Old hydroelectric power stations often provide unsafe migration routes to fish, apart from passing through mobile gates during high flow. The installation of retrofitted bypass is considered to be a potential solution to improve fish passage at such old structures, but their performances are often insufficient. In the Amblève River (Belgium), a mobile 3.3 m high dam feeds two principal Francis turbines
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Production of lipids by Chaetoceros affinis in media based on palm oil mill effluent J. Biotechnol. (IF 3.503) Pub Date : 2021-01-06 Karthick Murugan Palanisamy; Primilla Paramasivam; Gaanty Pragas Maniam; Mohd Hasbi Ab. Rahim; Natanamurugaraj Govindan; Yusuf Chisti
Biomass and lipid production by the marine diatom Chaetoceros affinis were characterized under continuous light with aeration. Media based on palm oil mill effluent (POME; 10, 20 and 30 % v/v in distilled water) were used together with a standard control medium. The maximum biomass concentration on day 12 of batch cultures in control medium was 821 ± 71 mg L–1. Under identical conditions, in the best
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From a plant secretion to the promising bone grafts: Cryogels of silicon-integrated quince seed mucilage by microwave-assisted sol–gel reaction J. Biosci. Bioeng. (IF 2.366) Pub Date : 2021-01-14 Hilal Deniz Yilmaz; Ugur Cengiz; Yavuz Emre Arslan; Fadime Kiran; Ahmet Ceylan
Design and fabrication of biologically active cryogels using novel biopolymer(s) are still of great importance at regenerating bone defects such as traumatic bone injuries, maxillofacial surgery, osteomyelitis, and osteoporosis. Nowadays, plant mucilage, an herbal biomaterial, has been drawn attention by scientists due to their marvelous potential to fabricate 3-dimensional (3D) physical constructs
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Metabolic engineering of E. coli for pyocyanin production Metab. Eng. (IF 7.263) Pub Date : 2021-01-14 Adilson José da Silva; Josivan de Souza Cunha; Teri Hreha; Kelli Cristina Micocci; Heloisa Sobreiro Selistre de Araujo; Blanca Barquera; Mattheos A.G. Koffas
Pyocyanin is a secondary metabolite from Pseudomonas aeruginosa that belongs to the class of phenazines, which are aromatic nitrogenous compounds with numerous biological functions. Besides its antifungal and antimicrobial activities, pyocyanin is a remarkable redox-active molecule with potential applications ranging from the pharma industry to the development of microbial fuel cells. Nevertheless
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Nanoencapsulation of clove oil and study of physicochemical properties, cytotoxic, hemolytic, and antioxidant activities J. Food Process Eng. (IF 1.703) Pub Date : 2021-01-13 Pramod G. Nagaraju; Parineeta Sengupta; Poornima Priyadarshini Chicgovinda; Pooja J. Rao
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Thermophysical properties of frozen parsley: A state diagram representation J. Food Process Eng. (IF 1.703) Pub Date : 2021-01-13 Eleonora Iaccheri; Chiara Cevoli; Marco Dalla Rosa; Angelo Fabbri
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A Proof‐of‐concept study of an automated solution for clinical metagenomic Next‐Generation Sequencing J. Appl. Microbiol. (IF 3.066) Pub Date : 2021-01-13 Yi Luan; Huiling Hu; Chao Liu; Bin Chen; Xiaoqiang Liu; Ying Xu; Xiaohong Luo; Jing Chen; Baochun Ye; Fei Huang; Jun Wang; Chaohui Duan
Metagenomic next‐generation sequencing (mNGS) has been utilized for diagnosing infectious diseases. It is a culture‐free and hypothesis‐free nucleic acid test for diagnosing all pathogens with known genomic sequences, including bacteria, fungi, viruses, and parasites. While this technique greatly expands the clinical capacity of pathogen detection, it is a second‐line choice due to lengthy procedures
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A counterintuitive method for slowing down an analyte in a nanopore and its application to polymer sequencing bioRxiv. Bioeng. Pub Date : 2021-01-13 G Sampath
A major obstacle faced by nanopore-based polymer sequencing and analysis is the high speed of translocation of an analyte (nucleotide, DNA, amino acid (AA), peptide) through the pore; the rate currently exceeds available detector bandwidth. Except for one method that uses an enzyme ratchet to sequence DNA, attempts to resolve the problem satisfactorily have been largely unsuccessful. Here a counterintuitive
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Biomaterials direct functional B cell response in a material specific manner bioRxiv. Bioeng. Pub Date : 2021-01-13 Erika Moore; David R Maestas; Christopher C Cherry; Jordan A Garcia; Hannah Y Comeau; Locke Davenport Huyer; Richard L. Blosser; Gedge D Rosson; Jennifer H. Elisseeff
B cells are an adaptive immune target of biomaterials development in vaccine research but despite their role in wound healing have not been studied in tissue engineering and regenerative medicine. We evaluated the B cell response to biomaterial scaffold materials implanted in a muscle wound; a biological extracellular matrix (ECM) and synthetic polyester polycaprolactone. In the local muscle tissue
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High-resolution structural and functional deep brain imaging using adaptive optics three-photon microscopy bioRxiv. Bioeng. Pub Date : 2021-01-13 Lina Streich; Juan Carlos Boffi; Ling Wang; Khaleel Alhalaseh; Matteo Barbieri; Ronja Rehm; Senthilkumar Deivasigamani; Cornelius Gross; Amit Agarwal; Robert Prevedel
Multi-photon microscopy has become a powerful tool to visualize the morphology and function of neural cells and circuits in the intact mammalian brain. Yet, tissue scattering, optical aberrations, and motion artifacts degrade the achievable image quality with depth. Here we developed a minimally invasive intravital imaging methodology by combining three-photon excitation, indirect adaptive optics correction
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Robust RNA editing via recruitment of endogenous ADARs using circular guide RNAs bioRxiv. Bioeng. Pub Date : 2021-01-13 Dhruva Katrekar; James Yen; Yichen Xiang; Anushka Saha; Dario Meluzzi; Yiannis Savva; Prashant Mali
Akin to short-hairpin RNAs and antisense oligonucleotides which efficaciously recruit endogenous cellular machinery such as Argonaute and RNase H to enable targeted RNA knockdown, simple long antisense guide RNAs (1) can recruit endogenous adenosine deaminases acting on RNA (ADARs) to enable programmable A-to-I RNA editing, without requiring co-delivery of any exogenous proteins. This approach is highly
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Flux redistribution of central carbon metabolism for efficient production of l‐tryptophan in Escherichia coli Biotechnol. Bioeng. (IF 4.002) Pub Date : 2021-01-05 Bo Xiong; Yongduo Zhu; Daoguang Tian; Shuai Jiang; Xiaoguang Fan; Qian Ma; Heyun Wu; Xixian Xie
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Real‐time ensemble microalgae growth forecasting with data assimilation Biotechnol. Bioeng. (IF 4.002) Pub Date : 2021-01-05 Hongxiang Yan; Mark S. Wigmosta; Ning Sun; Michael H. Huesemann; Song Gao