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Derivation of an Upscaled Model for Xylitol Production With Immobilized Microorganisms Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-02-17 Rolando Zenteno-Catemaxca, Roel Hernandez-Rodriguez, Epifanio Morales-Zárate, Eliseo Hernandez-Martinez
Xylitol, a polyalcohol with anticariogenic properties, finds broad applications in different sectors. Research into new production methods has highlighted the biochemical route using immobilized microorganisms. However, challenges remain in optimizing operating conditions and understanding mass transport and biochemical reactions. Different macroscopic models have been proposed to address these challenges
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Vertex’s opioid-free drug for acute pain wins FDA approval Nat. Biotechnol. (IF 33.1) Pub Date : 2025-02-17
Society, patients and clinicians welcome a much-needed non-opioid pain medication. Vertex’s first-in-class analgesic Journavx could soon be followed by a new generation of addiction-free pain drugs acting at NaV1.8 sodium channels.
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The reverse transcriptase domain of prime editors contributes to DNA repair in mammalian cells Nat. Biotechnol. (IF 33.1) Pub Date : 2025-02-17 Chunwei Zheng, Gangming Zhang, Lilia J. Dean, Erik J. Sontheimer, Wen Xue
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Antibodies Nat. Biotechnol. (IF 33.1) Pub Date : 2025-02-14
Recent patents relating to antibody compositions and methods of use.
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Biotech news from around the world Nat. Biotechnol. (IF 33.1) Pub Date : 2025-02-14
The UK government says it will introduce in parliament by the end of March the legislation needed to implement new precision breeding rules. The new law will allow gene-edited plants to be commercialized in the UK. In the European Union, gene editing and other precision breeding techniques are effectively banned for cultivation, as they fall under the same regulations as genetically modified crops
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Heat-resistant rice without yield compromise Nat. Biotechnol. (IF 33.1) Pub Date : 2025-02-14 Alexandra Despang
In the face of global warming, producing stress- and heat-resistant plants has been a long-standing goal in plant breeding and genetics. Strategies like transcriptional regulation and gene overexpression for thermotolerance often reduce crop yields. Now, in Nature Genetics, Lu et al. report a wax biosynthesis module that can be genetically engineered to enhance rice yield under heat stress without
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Gene and cell therapies for Parkinson’s make headway Nat. Biotechnol. (IF 33.1) Pub Date : 2025-02-14
An investigational gene therapy to treat Parkinson’s disease entered phase 2 studies in January. AskBio, a Bayer-owned company, will deploy an adeno-associated virus serotype 2 (AAV) delivered into the brain in randomized, double-blind studies in adults with moderate Parkinson’s. The AB-1005 therapy will deliver human glial cell line-derived neurotrophic factor (GDNF) genes into neurons to promote
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Largest proteome study enlists 14 biopharmas Nat. Biotechnol. (IF 33.1) Pub Date : 2025-02-14
Top biopharma companies have joined forces to conduct the biggest proteomics study yet, aimed at understanding how fluctuations in protein levels influence disease. The UK Biobank Pharma Proteomics Project will analyze 5,400 protein markers across 600,000 blood samples — half a million held in the UK Biobank from middle-aged participants recruited between 2006 and 2010, and 100,000 second samples taken
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De novo-designed protein binders neutralize snake toxins Nat. Biotechnol. (IF 33.1) Pub Date : 2025-02-14 Anahita Bishop
Three-finger toxins are the major component of the venom of elapid snakes and have limited immunogenicity in antivenom-producing animals, which results in a weak antibody response. Current treatments comprising polyclonal antibodies therefore often lack efficacy in preventing neurotoxicity and tissue necrosis. In a recent study published in Nature, Torres et al. use deep-learning methods to produce
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Startup grows egg proteins in potato fields Nat. Biotechnol. (IF 33.1) Pub Date : 2025-02-14
Ovalbumin, the main protein in egg whites, is sought after as an ingredient by food manufacturers as it helps to boost nutritional value and extend the shelf life of packaged products. The team engineered the potato by inserting the entire ovalbumin DNA sequence into the leaf, which thus contained instructions to produce a fully functional protein that is nutritionally and chemically identical to that
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A chromatin-based model for deciphering gene interactions Nat. Biotechnol. (IF 33.1) Pub Date : 2025-02-14 Rong Li
Foundation models have leveraged diverse gene expression profiles for tasks such as cell type annotation or prediction of perturbation response. However, no model yet exists to demonstrate how the chromatin landscape regulates transcription. Writing in Nature, Lu et al. introduce the general expression transformer (GET), a foundation model trained on chromatin accessibility data from diverse human
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Colossal raises $200 million to bring back the dodo Nat. Biotechnol. (IF 33.1) Pub Date : 2025-02-14
Real-life Jurassic Park startup Colossal BioSciences announced January 15 that it has raised $200 million to bring back three extinct animals: the woolly mammoth, the Tasmanian tiger and the dodo bird. Resurrecting an extinct ancient species would be a first that would require Colossal researchers to overcome some colossal challenges. The company says it has amassed the most continuous and complete
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People Nat. Biotechnol. (IF 33.1) Pub Date : 2025-02-14
Recent moves of note in and around the biotech and pharma industries.
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AI biotechs launch bioprospecting expeditions with Indigenous groups, agree to share benefits Nat. Biotechnol. (IF 33.1) Pub Date : 2025-02-14
Companies are striking unconventional benefit-sharing agreements with national governments to scour planet hotspots, gathering thousands of new DNA and protein sequences to develop into new, commercially useful molecules.
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Undergraduate education as catalyst for public–private partnership in biotechnology Nat. Biotechnol. (IF 33.1) Pub Date : 2025-02-14 Toine ten Broeke, Thijs Koorman, Sandra Crnko, Erik Voets, Pauline de Goeje, Michael Y. Schakelaar, Heggert G. Rebel, Emma W. Pijnappel, Jorine G. F. Sanders, Jan Meeldijk, Rian Terveer-Couperus, Esther L. Geven, Gönül Dilaver, Erik van Tilborg, Lucas M. P. Beekman, Lars Guelen, Niels Bovenschen
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Redefining antibody patent protection using paratope mapping and CDR-scanning Nat. Biotechnol. (IF 33.1) Pub Date : 2025-02-14 Soma S. R. Banik, Xiaoxiang Deng, Edgar Davidson, Ulrich Storz, Benjamin J. Doranz
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Nitroglycerin-responsive gene switch for the on-demand production of therapeutic proteins Nat. Biomed. Eng. (IF 26.8) Pub Date : 2025-02-14 Mohamed Mahameed, Shuai Xue, Benjamin Danuser, Ghislaine Charpin-El Hamri, Mingqi Xie, Martin Fussenegger
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Microfluidic technologies for enhancing the potency, predictability and affordability of adoptive cell therapies Nat. Biomed. Eng. (IF 26.8) Pub Date : 2025-02-14 Zongjie Wang, Shana O. Kelley
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Discovery, characterization, and application of chromosomal integration sites for stable heterologous gene expression in Rhodotorula toruloides. Metab. Eng. (IF 6.8) Pub Date : 2025-02-14 Hao Xu,Longyuan Shi,Aashutosh Girish Boob,Wooyoung Park,Shih-I Tan,Vinh Gia Tran,John Carl Schultz,Huimin Zhao
Rhodotorula toruloides is a non-model, oleaginous yeast uniquely suited to produce acetyl-CoA-derived chemicals. However, the lack of well-characterized genomic integration sites has impeded the metabolic engineering of this organism. Here we report a set of computationally predicted and experimentally validated chromosomal integration sites in R. toruloides. We first implemented an in silico platform
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Targeting vaccines to dendritic cells by mimicking the processing and presentation of antigens in xenotransplant rejection Nat. Biomed. Eng. (IF 26.8) Pub Date : 2025-02-13 Jinjin Wang, Yuxuan Zhang, Yaru Jia, Haonan Xing, Fengfei Xu, Bozhang Xia, Wenjia Lai, Yuan Yuan, Xianlei Li, Shaobo Shan, Junge Chen, Weisheng Guo, Jinchao Zhang, Aiping Zheng, Jinghong Li, Ningqiang Gong, Xing-Jie Liang
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Issatchenkia orientalis as a platform organism for cost-effective production of organic acids. Metab. Eng. (IF 6.8) Pub Date : 2025-02-13 Shih-I Tan,Zijun Liu,Vinh Gia Tran,Teresa Anne Martin,Huimin Zhao
Driven by the urgent need to reduce the reliance on fossil fuels and mitigate environmental impacts, microbial cell factories capable of producing value-added products from renewable resources have gained significant attention over the past few decades. Notably, non-model yeasts with unique physiological characteristics have emerged as promising candidates for industrial applications, particularly
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High-throughput multiplexed serology via the mass-spectrometric analysis of isotopically barcoded beads Nat. Biomed. Eng. (IF 26.8) Pub Date : 2025-02-12 Alexandros P. Drainas, David R. McIlwain, Alec Dallas, Theresa Chu, Antonio Delgado-González, Maya Baron, Maria Angulo-Ibáñez, Angelica Trejo, Yunhao Bai, John W. Hickey, Guolan Lu, Scott Lu, Jesus Pineda-Ramirez, Khamal Anglin, Eugene T. Richardson, John C. Prostko, Edwin Frias, Venice Servellita, Noah Brazer, Charles Y. Chiu, Michael J. Peluso, Jeffrey N. Martin, Oliver F. Wirz, Tho D. Pham, Scott
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A gut-on-a-chip incorporating human faecal samples and peristalsis predicts responses to immune checkpoint inhibitors for melanoma Nat. Biomed. Eng. (IF 26.8) Pub Date : 2025-02-12 Mattia Ballerini, Serena Galiè, Punit Tyagi, Carlotta Catozzi, Hariam Raji, Amir Nabinejad, Angeli D. G. Macandog, Alessandro Cordiale, Bianca Ionela Slivinschi, Karol K. Kugiejko, Martina Freisa, Paola Occhetta, Jennifer A. Wargo, Pier F. Ferrucci, Emilia Cocorocchio, Nicola Segata, Andrea Vignati, Andrey Morgun, Michela Deleidi, Teresa Manzo, Marco Rasponi, Luigi Nezi
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Unlocking the Potential of Shewanella in Metabolic Engineering: Current Status, Challenges, and Opportunities. Metab. Eng. (IF 6.8) Pub Date : 2025-02-12 Yi-Nan Liu,Zhourui Liu,Jian Liu,Yidan Hu,Bin Cao
Shewanella species are facultative anaerobes with distinctive electrochemical properties, making them valuable for applications in energy conversion and environmental bioremediation. Due to their well-characterized electron transfer mechanisms and ease of genetic manipulation, Shewanella spp. have emerged as promising chassis for metabolic engineering. In this review, we provide a comprehensive overview
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Development of Methods to Produce SARS CoV‐2 Virus‐Like Particles at Scale Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-02-12 Melissa A. Edeling, Linda Earnest, Julio Carrera Montoya, Ashley Huey Yiing Yap, Jamie Mumford, Jason Roberts, Chinn Yi Wong, Dhiraj Hans, Joseph Grima, Nicole Bisset, Jesse Bodle, Steven Rockman, Joseph Torresi
The devastating global toll precipitated by the SARS CoV‐2 outbreak and the profound impact of vaccines in stemming that outbreak has established the need for molecular platforms capable of rapidly delivering effective, safe and accessible medical interventions in pandemic preparedness. We describe a simple, efficient and adaptable process to produce SARS CoV‐2 virus‐like particles (VLPs) that can
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Zeolitic Imidazole Framework‐8 Nanoparticles as an Alternative to Freund's Adjuvant for Klebsiella pneumoniae Recombinant Protein Vaccine Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-02-12 Gaowei Hu, Chunli Hong, Yingjie Miao, Wenji Wang, Longfei Yin, Xi Luo, Yongqian Fu
Vaccination represents a promising approach to combat resistant Klebsiella pneumoniae (KP). However, there is currently no licensed vaccine in the veterinary field. Outer membrane proteins have been proven to possess good immunogenicity, but Freund's adjuvant, which is commonly used to administer protein vaccines, has limitations such as a complicated formulation process as well as a tendency to cause
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Gains in early-onset dementia with progranulin open new paths for drug discovery Nat. Biotechnol. (IF 33.1) Pub Date : 2025-02-11
The first clinical results in patients with a genetic form of frontotemporal dementia (FTD) show that enhancing progranulin in the brain may halt disease progression. If successful, this potentially disease-modifying approach may uncover new avenues for treating other neurodegenerative diseases.
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Precise RNA targeting with CRISPR–Cas13d Nat. Biotechnol. (IF 33.1) Pub Date : 2025-02-11 Sydney K. Hart, Simon Müller, Hans-Hermann Wessels, Alejandro Méndez-Mancilla, Gediminas Drabavicius, Olivia Choi, Neville E. Sanjana
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Synaptic connectivity mapping among thousands of neurons via parallelized intracellular recording with a microhole electrode array Nat. Biomed. Eng. (IF 26.8) Pub Date : 2025-02-11 Jun Wang, Woo-Bin Jung, Rona S. Gertner, Hongkun Park, Donhee Ham
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Metabolic division engineering of Escherichia coli consortia for de novo biosynthesis of flavonoids and flavonoid glycosides. Metab. Eng. (IF 6.8) Pub Date : 2025-02-11 Zetian Qiu,Yumei Han,Jia Li,Yi Ren,Xue Liu,Shengying Li,Guang-Rong Zhao,Lei Du
Heterologous biosynthesis of natural products with long biosynthetic pathways in microorganisms often suffers from diverse problems, such as enzyme promiscuity and metabolic burden. Flavonoids and their glycosides are important phytochemicals in the diet of human beings, with various health benefits and biological activities. Despite previous efforts and achievements, efficient microbial production
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Enhanced Natural Killer Cell Proliferation by Stress‐Induced Feeder Cells Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-02-11 Donghyun Lee, Myeongkwan Song, Soonjo Kwon
Natural killer (NK) cells, integral to the innate immune system, are notable in cell therapies because of their applicability in allogeneic treatments, distinguishing them from T cells typically employed in conventional cell therapies. However, their limited half‐life (proliferative capability) poses a challenge for therapy. The limited half‐life creates difficulties in obtaining a sufficient number
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Assessing the Adjuvant Potential of Chinese Hamster Ovary Host Cell Proteins Using an In Vitro Dendritic Cell Assay Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-02-11 Sherin Panikulam, Hannah Morgan, Michael Gutknecht, Anette Karle, Atchaya Rajaratnam, Jennifer Muntwyler, Oliver Anderka, Nicolas Lebesgue, Thomas K. Villiger
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Biotechnology and Bioengineering: Volume 122, Number 3, March 2025 Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-02-11
Click on the article title to read more.
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Chimeric enzymes in the pulp and paper making industry: Current developments Biotechnol. Adv. (IF 12.1) Pub Date : 2025-02-09 Abdullah Arsalan, Yuvaraj Ravikumar, Xinrui Tang, Zijing Cao, Mei Zhao, Wenjing Sun, Xianghui Qi
The pulp and paper (P&P) industry plays a vital role in supporting the social and economic progress of a country by supplying essential commodities. Conventional P&P processing often consumes significant energy and use chemical agents to produce hazardous intermediates. The use of enzymes in the P&P industry has significantly reduced both the chemical and energy demands during processing. A variety
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Small circular RNAs as vaccines for cancer immunotherapy Nat. Biomed. Eng. (IF 26.8) Pub Date : 2025-02-07 Yu Zhang, Xiang Liu, Tingting Shen, Qiyan Wang, Shurong Zhou, Suling Yang, Shimiao Liao, Ting Su, Lei Mei, Bei Zhang, Khoa Huynh, Linying Xie, Youzhong Guo, Chunqing Guo, Katarzyna M. Tyc, Xufeng Qu, Xiang-Yang Wang, Jinze Liu, Guizhi Zhu
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Computational advances in biosynthetic gene cluster discovery and prediction Biotechnol. Adv. (IF 12.1) Pub Date : 2025-02-07 Sisi Zhu, Hongquan Xu, Yuhong Liu, Yanfeng Hong, Haowen Yang, Changli Zhou, Lin Tao
Biosynthetic gene clusters (BGCs) are groups of clustered genes found in bacteria, fungi, and some plants and animals that are crucial for synthesizing secondary metabolites. In recent years, genome mining of BGCs has emerged as a prominent research focus, particularly in natural product discovery and drug development. Compared to traditional experimental methods, applying computational techniques
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Enhancing Biomass Productivity by Forecast‐Informed Pond Operations Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-02-07 Hongxiang Yan, Mark S. Wigmosta, Ning Sun, Song Gao, Michael H. Huesemann
Microalgal cultivation for biofuels and proteins holds significant promise but faces challenges in achieving economically viable biomass productivity under variable environmental conditions. This study introduces a forecast‐informed pond operation (FIPO) system that uses numerical weather prediction (NWP) ensemble forecasts and the biomass assessment tool (BAT) to optimize daily dilution rates for
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One‐Pot Hetero‐Di‐C‐Glycosylation of the Natural Polyphenol Phloretin by a Single C‐Glycosyltransferase With Broad Sugar Substrate Specificity Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-02-07 Tuo Li, Annika J. E. Borg, Leo Krammer, Rolf Breinbauer, Bernd Nidetzky
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Polyhydroxyalkanoates production from microalgae for sustainable bioplastics: A review Biotechnol. Adv. (IF 12.1) Pub Date : 2025-02-06 Syarifa Ilhami, Siti Nur Syaza Abdul Rahman, Muhammad Iqhrammullah, Zhafran Hamid, Yee Ho Chai, Man Kee Lam
Microalgae have emerged as a promising and sustainable source for polyhydroxyalkanoates (PHA), which are increasingly recognized for their potential in bioplastics production. However, the widespread application of microalgae-derived PHA faces challenges related to economic feasibility and scalability. This review provides a comprehensive analysis of recent advancements in the cultivation and optimization
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Systematic Development of a Detergent Toolbox as an Alternative to Triton X‐100 Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-02-06 Varsha Yadav, Mary Lunson, Hannah Shore, Jean Aucamp
Detergents are routinely included in protein purification processes to inactivate enveloped viruses that may arise from adventitious or endogenous contamination. The detergent Triton X‐100 (TX‐100) has been widely used as part of the production process for therapeutic proteins. However, recent ecological studies indicate that TX‐100 and its metabolites detrimentally impact aquatic organisms, thus alternative
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Construction of a CRISPR‐Cas9‐Based Genetic Editing Tool for Serratia marcescens Using a Stationary Phase Promoter and Its Application in Putrescine Production Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-02-06 Linbo Gou, Di Liu, Tai‐Ping Fan, Huaxiang Deng, Yujie Cai
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SEED-Selection enables high-efficiency enrichment of primary T cells edited at multiple loci Nat. Biotechnol. (IF 33.1) Pub Date : 2025-02-05 Christopher R. Chang, Vivasvan S. Vykunta, Jae Hyun J. Lee, Ke Li, Clara Kochendoerfer, Joseph J. Muldoon, Charlotte H. Wang, Thomas Mazumder, Yang Sun, Daniel B. Goodman, William A. Nyberg, Chang Liu, Vincent Allain, Allison Rothrock, Chun J. Ye, Alexander Marson, Brian R. Shy, Justin Eyquem
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Safer non-viral DNA delivery using lipid nanoparticles loaded with endogenous anti-inflammatory lipids Nat. Biotechnol. (IF 33.1) Pub Date : 2025-02-05 Manthan N. Patel, Sachchidanand Tiwari, Yufei Wang, Sarah O’Neill, Jichuan Wu, Serena Omo-Lamai, Carolann Espy, Liam S. Chase, Aparajeeta Majumder, Evan Hoffman, Anit Shah, András Sárközy, Jeremy Katzen, Norbert Pardi, Jacob S. Brenner
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Sound healing and beyond Nat. Biotechnol. (IF 33.1) Pub Date : 2025-02-04
Ultrasound neurotechnologies are moving quickly into clinical trials in a wide variety of applications, and initiatives to open-source their manufacture will make them more accessible.
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Implantation of engineered adipocytes suppresses tumor progression in cancer models Nat. Biotechnol. (IF 33.1) Pub Date : 2025-02-04 Hai P. Nguyen, Kelly An, Yusuke Ito, Bhushan N. Kharbikar, Rory Sheng, Breanna Paredes, Elizabeth Murray, Kimberly Pham, Michael Bruck, Xujia Zhou, Cassandra Biellak, Aki Ushiki, Mai Nobuhara, Sarah L. Fong, Daniel A. Bernards, Filipa Lynce, Deborah A. Dillon, Mark Jesus M. Magbanua, Laura A. Huppert, Heinz Hammerlindl, Jace Anton Klein, Luis Valdiviez, Oliver Fiehn, Laura Esserman, Tejal A. Desai
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Quantifying metabolites using structure-switching aptamers coupled to DNA sequencing Nat. Biotechnol. (IF 33.1) Pub Date : 2025-02-04 June H. Tan, Andrew G. Fraser
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Author Correction: Restoration of tumour-growth suppression in vivo via systemic nanoparticle-mediated delivery of PTENmRNA Nat. Biomed. Eng. (IF 26.8) Pub Date : 2025-02-05 Mohammad Ariful Islam, Yingjie Xu, Wei Tao, Jessalyn M. Ubellacker, Michael Lim, Daniel Aum, Gha Young Lee, Kun Zhou, Harshal Zope, Mikyung Yu, Wuji Cao, James Trevor Oswald, Meshkat Dinarvand, Morteza Mahmoudi, Robert Langer, Philip W. Kantoff, Omid C. Farokhzad, Bruce R. Zetter, Jinjun Shi
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Evolving adeno-associated viruses for gene transfer to the kidney via cross-species cycling of capsid libraries Nat. Biomed. Eng. (IF 26.8) Pub Date : 2025-02-05 Alan Rosales, Leo O. Blondel, Joshua Hull, Qimeng Gao, Nihal Aykun, Jennifer L. Peek, Alejandra Vargas, Sophia Fergione, Mingqing Song, Matthew H. Wilson, Andrew S. Barbas, Aravind Asokan
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Author Correction: A microfluidic assay for the quantification of the metastatic propensity of breast cancer specimens Nat. Biomed. Eng. (IF 26.8) Pub Date : 2025-02-05 Christopher L. Yankaskas, Keyata N. Thompson, Colin D. Paul, Michele I. Vitolo, Panagiotis Mistriotis, Ankit Mahendra, Vivek K. Bajpai, Daniel J. Shea, Kristen M. Manto, Andreas C. Chai, Navin Varadarajan, Aikaterini Kontrogianni-Konstantopoulos, Stuart S. Martin, Konstantinos Konstantopoulos
Correction to: Nature Biomedical Engineering https://doi.org/10.1038/s41551-019-0400-9, published online 06 May 2019.
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Unleashing the potential of microbial biosynthesis of monoterpenes via enzyme and metabolic engineering Biotechnol. Adv. (IF 12.1) Pub Date : 2025-02-05 Dianqi Yang, Hong Liang, Xuxu Li, Chenyu Zhang, Zewei Lu, Xiaoqiang Ma
Monoterpenes (MTPs) are valuable isoprenoids widely used in cosmetics, food flavorings, pharmaceuticals, etc. Compared to plant extraction and chemical synthesis, microbial biosynthesis offers superior sustainability and efficiency in producing natural MTPs, overcoming the limitations of raw material dependency, environmental impact, and racemic mixtures inherent in these methods. This review comprehensively
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Demonstrating the Effectiveness of an Alternative to Triton X‐100 for Detergent‐Mediated Viral Inactivation in Biomanufacturing Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-02-05 Kakolie Banerjee, Alice Antonello, Sandra Johnson, Anja Licht, Almut Rapp, Corinne Miller
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Chromatography in Downstream Processing of Recombinant Adeno‐Associated Viruses: A Review of Current and Future Practises Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-02-05 Julius Klemens Lorek, Madelène Isaksson, Bernt Nilsson
Recombinant adeno‐associated virus (rAAV) has emerged as an attractive gene delivery vector platform to treat both rare and pervasive diseases. With more and more rAAV‐based therapies entering late‐stage clinical trials and commercialization, there is an increasing pressure on the rAAV manufacturing process to accelerate drug development, account for larger trials, and commercially provide high doses
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Author Correction: Multiplexed inhibition of immunosuppressive genes with Cas13d for combinatorial cancer immunotherapy Nat. Biotechnol. (IF 33.1) Pub Date : 2025-02-03 Feifei Zhang, Ryan D. Chow, Emily He, Chuanpeng Dong, Shan Xin, Daniyal Mirza, Yanzhi Feng, Xiaolong Tian, Nipun Verma, Medha Majety, Yueqi Zhang, Guangchuan Wang, Sidi Chen
Correction to: Nature Biotechnology https://doi.org/10.1038/s41587-024-02535-2, published online 16 January 2025.
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Author Correction: Bioinstructive implantable scaffolds for rapid in vivo manufacture and release of CAR-T cells Nat. Biotechnol. (IF 33.1) Pub Date : 2025-02-03 Pritha Agarwalla, Edikan A. Ogunnaike, Sarah Ahn, Kristen A. Froehlich, Anton Jansson, Frances S. Ligler, Gianpietro Dotti, Yevgeny Brudno
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Cellular variability as a driver for bioprocess innovation and optimization Biotechnol. Adv. (IF 12.1) Pub Date : 2025-02-04 M. Eigenfeld, S.P. Schwaminger
Cellular heterogeneity plays a crucial role in biotechnological processes, significantly influencing metabolic activity, product yield, and process consistency. This review explores the different dimensions of cellular heterogeneity, focusing on its manifestation at both single-cell and population levels. The study examines how factors such as asymmetric cell division, age, and environmental conditions
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Unlocking the full potential of plant cell-based production for valuable proteins: Challenges and innovative strategies Biotechnol. Adv. (IF 12.1) Pub Date : 2025-02-04 Jianfeng Xu, Paula PerezSanchez, Shekoofeh Sadravi
Plant cell-based bioproduction systems offer a promising platform for the sustainable production of valuable proteins as they provide distinctive advantages over mammalian cell culture and whole plant cultivation. However, significant technical challenges remain, including low productivity, altered efficacy of plant-derived proteins, along with issues in culture process development, such as cell clumping
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Towards the application of nature's catalytic nanomachines: Cellulosomes in 2nd generation biofuel production Biotechnol. Adv. (IF 12.1) Pub Date : 2025-01-31 Maša Vodovnik, Nataša Lindič
Cellulosomes are highly efficient, complex multi-enzyme assemblies, predominantly found in anaerobic bacteria, which offer substantial potential for second-generation biofuel production through efficient lignocellulosic biomass degradation, thus reducing the need for costly pretreatments. Recent advances in cellulosome research have significantly contributed to developing more efficient consolidated
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Machine Learning‐Powered Optimization of a CHO Cell Cultivation Process Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-01-31 Jannik Richter, Qimin Wang, Ferdinand Lange, Phil Thiel, Nina Yilmaz, Dörte Solle, Xiaoying Zhuang, Sascha Beutel