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Peroxidasin is required for full viability in development and for maintenance of tissue mechanics in adults Matrix Biol. (IF 6.9) Pub Date : 2023-11-23 K. Elkie Peebles, Kimberly S. LaFever, Patrick S. Page-McCaw, Selene Colon, Dan Wang, Aubrie M. Stricker, Nicholas Ferrell, Gautam Bhave, Andrea Page-McCaw
Basement membranes are thin strong sheets of extracellular matrix. They provide mechanical and biochemical support to epithelia, muscles, nerves, and blood vessels, among other tissues. The mechanical properties of basement membranes are conferred in part by Collagen IV (Col4), an abundant protein of basement membranes that forms an extensive two-dimensional network through head-to-head and tail-to-tail
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Fiber alignment in 3D collagen networks as a biophysical marker for cell contractility Matrix Biol. (IF 6.9) Pub Date : 2023-11-14 David Böhringer, Andreas Bauer, Ivana Moravec, Lars Bischof, Delf Kah, Christoph Mark, Thomas J. Grundy, Ekkehard Görlach, Geraldine M O’Neill, Silvia Budday, Pamela L. Strissel, Reiner Strick, Andrea Malandrino, Richard Gerum, Michael Mak, Martin Rausch, Ben Fabry
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TGF-β induces matrisome pathological alterations and EMT in patient-derived prostate cancer tumoroids Matrix Biol. (IF 6.9) Pub Date : 2023-11-08 Soraia Fernandes, Jorge Oliver-De La Cruz, Sofia Morazzo, Francesco Niro, Marco Cassani, Helena Ďuríková, Alessio Caravella, Piergiuseppe Fiore, Giulia Azzato, Giuseppe De Marco, Agostino Lauria, Valerio Izzi, Veronika Bosáková, Jan Fric, Petr Filipensky, Giancarlo Forte
Extracellular matrix (ECM) tumorigenic alterations resulting in high matrix deposition and stiffening are hallmarks of adenocarcinomas and are collectively defined as desmoplasia. Here, we thoroughly analysed primary prostate cancer tissues obtained from numerous patients undergoing radical prostatectomy to highlight reproducible structural changes in the ECM leading to the loss of the glandular architecture
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Targeting the αVβ3/NgR2 pathway in neuroendocrine prostate cancer Matrix Biol. (IF 6.9) Pub Date : 2023-11-11 Anna Testa, Fabio Quaglia, Nicole M. Naranjo, Cecilia E. Verrillo, Christopher D. Shields, Stephen Lin, Maxwell W. Pickles, Drini F. Hamza, Tami Von Schalscha, David A. Cheresh, Benjamin Leiby, Qin Liu, Jianyi Ding, William K. Kelly, D. Craig Hooper, Eva Corey, Edward F. Plow, Dario C. Altieri, Lucia R. Languino
Highly aggressive, metastatic, neuroendocrine prostate cancer, which typically develops from prostate cancer cells acquiring resistance to androgen deprivation therapy, is associated with limited treatment options and hence poor prognosis. We have previously demonstrated that the αVβ3 integrin is over-expressed in neuroendocrine prostate cancer. We now show that LM609, a monoclonal antibody that specifically
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Age related changes in hyaluronan expression leads to Meibomian gland dysfunction Matrix Biol. (IF 6.9) Pub Date : 2023-11-08 Sudhir Verma, Isabel Y. Moreno, Mingxia Sun, Tarsis Ferreira Gesteira, Vivien J. Coulson-Thomas
The prevalence of dry eye disease (DED) ranges from ∼5 to 50 % and its associated symptoms decrease productivity and reduce the quality of life. Approximately 85 % of all DED cases are caused by Meibomian gland dysfunction (MGD). As humans and mice age, their Meibomian glands (MGs) undergo age-related changes resulting in age related-MGD (ARMGD). The precise cause of ARMGD remains elusive, which makes
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Pulmonary fibroelastosis - A review Matrix Biol. (IF 6.9) Pub Date : 2023-11-03 Dan J.K. Yombo, Satish K. Madala, Chanukya P. Vemulapalli, Harshavardhana H. Ediga, William D. Hardie
Elastin is a long-lived fibrous protein that is abundant in the extracellular matrix of the lung. Elastic fibers provide the lung the characteristic elasticity during inhalation with recoil during exhalation thereby ensuring efficient gas exchange. Excessive deposition of elastin and other extracellular matrix proteins reduces lung compliance by impairing ventilation and compromising gas exchange.
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Mmp14 is required for matrisome homeostasis and circadian rhythm in fibroblasts Matrix Biol. (IF 6.9) Pub Date : 2023-10-31 Ching-Yan Chloé Yeung, Richa Garva, Adam Pickard, Yinhui Lu, Venkatesh Mallikarjun, Joe Swift, Susan H. Taylor, Jyoti Rai, David R. Eyre, Mayank Chaturvedi, Yoshifumi Itoh, Qing-Jun Meng, Cornelia Mauch, Paola Zigrino, Karl E. Kadler
The circadian clock in tendon regulates the daily rhythmic synthesis of collagen-I and the appearance and disappearance of small-diameter collagen fibrils in the extracellular matrix. How the fibrils are assembled and removed is not fully understood. Here, we first showed that the collagenase, membrane type I-matrix metalloproteinase (MT1-MMP, encoded by Mmp14), is regulated by the circadian clock
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Tissue inhibitors of metalloproteinases are proteolytic targets of matrix metalloproteinase 9 Matrix Biol. (IF 6.9) Pub Date : 2023-10-06 Sasha Coates-Park, Carolyn Lazaroff, Sadeechya Gurung, Josh Rich, Alexandra Colladay, Maura O'Neill, Georgina S. Butler, Christopher M. Overall, William G. Stetler-Stevenson, David Peeney
Extracellular proteolysis and turnover are core processes of tissue homeostasis. The predominant matrix-degrading enzymes are members of the Matrix Metalloproteinase (MMP) family. MMPs extensively degrade core matrix components in addition to processing a range of other factors in the extracellular, plasma membrane, and intracellular compartments. The proteolytic activity of MMPs is modulated by the
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Molecular cues for immune cells from small leucine-rich repeat proteoglycans in their extracellular matrix-associated and free forms Matrix Biol. (IF 6.9) Pub Date : 2023-10-02 George Maiti, Sean Ashworth, Tansol Choi, Shukti Chakravarti
In this review we highlight emerging immune regulatory functions of lumican, keratocan, fibromodulin, biglycan and decorin, which are members of the small leucine-rich proteoglycans (SLRP) of the extracellular matrix (ECM). These SLRPs have been studied extensively as collagen-fibril regulatory structural components of the skin, cornea, bone and cartilage in homeostasis. However, SLRPs released from
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Inhibition of hyaluronan synthesis prevents β-cell loss in obesity-associated type 2 diabetes Matrix Biol. (IF 6.9) Pub Date : 2023-09-30 Nadine Nagy, Gernot Kaber, Vivekananda G. Sunkari, Payton L. Marshall, Aviv Hargil, Hedwich F. Kuipers, Heather D. Ishak, Marika Bogdani, Rebecca L. Hull, Maria Grandoch, Jens W. Fischer, Tracey L. McLaughlin, Thomas N. Wight, Paul L. Bollyky
Pancreatic β-cell dysfunction and death are central to the pathogenesis of type 2 diabetes (T2D). We identified a novel role for the inflammatory extracellular matrix polymer hyaluronan (HA) in this pathophysiology. Low concentrations of HA were present in healthy pancreatic islets. However, HA substantially accumulated in cadaveric islets of T2D patients and islets of the db/db mouse model of T2D
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Unraveling the role of TGFβ signaling in thoracic aortic aneurysm and dissection using Fbn1 mutant mouse models Matrix Biol. (IF 6.9) Pub Date : 2023-09-06 Violette Deleeuw, Eric Carlson, Marjolijn Renard, Keith D. Zientek, Phillip A. Wilmarth, Ashok P. Reddy, Elise C. Manalo, Sara F. Tufa, Douglas R. Keene, Margie Olbinado, Marco Stampanoni, Sachiko Kanki, Hiromi Yanagisawa, Laura Muiño Mosquera, Patrick Sips, Julie De Backer, Lynn Y. Sakai
Although abnormal TGFβ signaling is observed in several heritable forms of thoracic aortic aneurysms and dissections including Marfan syndrome, its precise role in aortic disease progression is still disputed. Using a mouse genetic approach and quantitative isobaric labeling proteomics, we sought to elucidate the role of TGFβ signaling in three Fbn1 mutant mouse models representing a range of aortic
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En route towards a personalized medicine approach: Innovative therapeutic modalities for connective tissue disorders Matrix Biol. (IF 6.9) Pub Date : 2023-08-30 Charlene Redhead, Nandaraj Taye, Dirk Hubmacher
Connective tissue disorders can be caused by pathogenic variants (mutations) in genes encoding extracellular matrix (ECM) proteins. Such disorders typically manifest during development or postnatal growth and result in significant morbidity and mortality. The development of curative treatments for connective tissue disorders is hampered in part by the inability of many mature connective tissues to
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Autocrine IL-6 drives cell and extracellular matrix anisotropy in scar fibroblasts Matrix Biol. (IF 6.9) Pub Date : 2023-09-01 Fiona N. Kenny, Stefania Marcotti, Deandra Belo De Freitas, Elena M. Drudi, Vivienne Leech, Rachel E. Bell, Jennifer Easton, María-del-Carmen Díaz-de-la-Loza, Roland Fleck, Leanne Allison, Christina Philippeos, Angelika Manhart, Tanya J. Shaw, Brian M. Stramer
Fibrosis is associated with dramatic changes in extracellular matrix (ECM) architecture of unknown etiology. Here we exploit keloid scars as a paradigm to understand fibrotic ECM organization. We reveal that keloid patient fibroblasts uniquely produce a globally aligned ECM network in 2-D culture as observed in scar tissue. ECM anisotropy develops after rapid initiation of a fibroblast supracellular
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Global, but not chondrocyte-specific, MT1-MMP deficiency in adult mice causes inflammatory arthritis Matrix Biol. (IF 6.9) Pub Date : 2023-08-19 Xiao-dan Xia, Govind Gill, Haiming Lin, Daniela M. Roth, Hong-mei Gu, Xiang-jiang Wang, Feng-yi Su, Adekunle Alabi, Maria Alexiou, Ziyang Zhang, Gui-qing Wang, Daniel Graf, Da-wei Zhang
Membrane-type I metalloproteinase (MT1-MMP/MMP14) plays a key role in various pathophysiological processes, indicating an unaddressed need for a targeted therapeutic approach. However, mice genetically deficient in Mmp14 show severe defects in development and growth. To investigate the possibility of MT1-MMP inhibition as a safe treatment in adults, we generated global Mmp14 tamoxifen-induced conditional
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Emilin2 fosters vascular stability by promoting pericyte recruitment Matrix Biol. (IF 6.9) Pub Date : 2023-08-12 Albina Fejza, Lucrezia Camicia, Greta Carobolante, Evelina Poletto, Alice Paulitti, Giorgia Schinello, Emanuele Di Siena, Renato Cannizzaro, Renato V. Iozzo, Gustavo Baldassarre, Eva Andreuzzi, Paola Spessotto, Maurizio Mongiat
Angiogenesis, the formation of the new blood vessels from pre-existing vasculature, is an essential process occurring under both normal and pathological conditions, such as inflammation and cancer. This complex process is regulated by several cytokines, growth factors and extracellular matrix components modulating endothelial cell and pericyte function. In this study, we discovered that the extracellular
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The extracellular matrix – immune microenvironment crosstalk in cancer therapy: Challenges and opportunities Matrix Biol. (IF 6.9) Pub Date : 2023-07-29 Lara Closset, Okan Gultekin, Sahar Salehi, Dhifaf Sarhan, Kaisa Lehti, Jordi Gonzalez-Molina
Targeting the tumour immune microenvironment (TIME) by cancer immunotherapy has led to improved patient outcomes. However, response to these treatments is heterogeneous and cancer-type dependant. The therapeutic activity of classical cancer therapies such as chemotherapy, radiotherapy, and surgical oncology is modulated by alterations of the TIME. A major regulator of immune cell function and resistance
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SIRT4 protects against intestinal fibrosis by facilitating GLS1 degradation Matrix Biol. (IF 6.9) Pub Date : 2023-08-02 Xinru Xue, Xi Zeng, Xiaoqian Wu, Kexin Mu, Yue Dai, Zhifeng Wei
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The clock transcription factor BMAL1 is a key regulator of extracellular matrix homeostasis and cell fate in the intervertebral disc Matrix Biol. (IF 6.9) Pub Date : 2023-07-24 Michal Dudek, Honor Morris, Natalie Rogers, Dharshika RJ Pathiranage, Sujitha Saba Raj, Danny Chan, Karl E Kadler, Judith Hoyland, Qing-Jun Meng
The circadian clock in mammals temporally coordinates physiological and behavioural processes to anticipate daily rhythmic changes in their environment. Chronic disruption to circadian rhythms (e.g., through ageing or shift work) is thought to contribute to a multitude of diseases, including degeneration of the musculoskeletal system. The intervertebral disc (IVD) in the spine contains circadian clocks
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Peptide location fingerprinting identifies structural alterations within basement membrane components in ageing kidney Matrix Biol. (IF 6.9) Pub Date : 2023-07-10 Alexander Eckersley, Mychel RPT Morais, Matiss Ozols, Rachel Lennon
During ageing, the glomerular and tubular basement membranes (BM) of the kidney undergo a progressive decline in function that is underpinned by histological changes, including glomerulosclerosis and tubular interstitial fibrosis and atrophy. This BM-specific ageing is thought to result from damage accumulation to long-lived extracellular matrix (ECM) protein structures. Determining which BM proteins
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SEMA7a primes integrin α5β1 engagement instructing fibroblast mechanotransduction, phenotype and transcriptional programming Matrix Biol. (IF 6.9) Pub Date : 2023-07-07 Hu P, Miller AE, Yeh CR, Bingham GC, Civelek M, Barker TH
Integrins are cellular receptors that bind the extracellular matrix (ECM) and facilitate the transduction of biochemical and biophysical microenvironment cues into cellular responses. Upon engaging the ECM, integrin heterodimers must rapidly strengthen their binding with the ECM, resulting in the assembly of force-resistant and force-sensitive integrin associated complexes (IACs). The IACs constitute
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Fibrolamellar carcinomas–growth arrested by paracrine signals complexed with synthesized 3-O sulfated heparan sulfate oligosaccharides Matrix Biol. (IF 6.9) Pub Date : 2023-07-02 Wencheng Zhang, Yongmei Xu, Xicheng Wang, Tsunekazu Oikawa, Guowei Su, Eliane Wauthier, Guoxiu Wu, Praveen Sethupathy, Zhiying He, Jian Liu, Lola M. Reid
Fibrolamellar carcinomas (FLCs), lethal tumors occurring in children to young adults, have genetic signatures implicating derivation from biliary tree stem cell (BTSC) subpopulations, co-hepato/pancreatic stem cells, involved in hepatic and pancreatic regeneration. FLCs and BTSCs express pluripotency genes, endodermal transcription factors, and stem cell surface, cytoplasmic and proliferation biomarkers
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TSG6 hyaluronan matrix remodeling dampens the inflammatory response during colitis Matrix Biol. (IF 6.9) Pub Date : 2023-06-29 Nansy Albtoush, Kimberly A. Queisser, Ash Zawerton, Mark E. Lauer, Ellen J. Beswick, Aaron C Petrey
In response to tissue injury, changes in the extracellular matrix (ECM) can directly affect the inflammatory response and contribute to disease progression or resolution. During inflammation, the glycosaminoglycan hyaluronan (HA) becomes modified by tumor necrosis factor stimulated gene-6 (TSG6). TSG6 covalently transfers heavy chain (HC) proteins from inter-α-trypsin inhibitor (IαI) to HA in a transesterification
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Absence of TRIC-B from type XIV Osteogenesis Imperfecta osteoblasts alters cell adhesion and mitochondrial function – A multi-omics study Matrix Biol. (IF 6.9) Pub Date : 2023-06-20 Milena Jovanovic, Apratim Mitra, Roberta Besio, Barbara Maria Contento, Ka Wai Wong, Alberta Derkyi, Michael To, Antonella Forlino, Ryan K Dale, Joan C Marini
Osteogenesis Imperfecta (OI) is a heritable collagen-related bone dysplasia characterized by bone fractures, growth deficiency and skeletal deformity. Type XIV OI is a recessive OI form caused by null mutations in TMEM38B, which encodes the ER membrane intracellular cation channel TRIC-B. Previously, we showed that absence of TMEM38B alters calcium flux in the ER of OI patient osteoblasts and fibroblasts
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ADAMTS3 restricts cancer invasion in models of early breast cancer progression through enhanced fibronectin degradation Matrix Biol. (IF 6.9) Pub Date : 2023-06-17 Shayin V. Gibson, Elizabeta Madzharova, Amandine C. Tan, Michael D. Allen, Ulrich auf dem Keller, J. Louise Jones, Edward P. Carter, Richard P. Grose
Proteases have long been associated with cancer progression, due to their ability to facilitate invasion upon matrix remodelling. However, proteases are not simply degraders of the matrix, but also play fundamental roles in modulating cellular behaviour through the proteolytic processing of specific substrates. Indeed, proteases can elicit both pro- and anti- tumorigenic effects depending on context
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Cell differentiation and matrix organization are differentially affected during bone formation in osteogenesis imperfecta zebrafish models with different genetic defects impacting collagen type I structure Matrix Biol. (IF 6.9) Pub Date : 2023-06-17 Valentina Daponte, Francesca Tonelli, Cecilia Masiero, Delfien Syx, Chloé Exbrayat-Héritier, Marco Biggiogera, Andy Willaert, Antonio Rossi, Paul J. Coucke, Florence Ruggiero, Antonella Forlino
Osteogenesis imperfecta (OI) is a family of rare heritable skeletal disorders associated with dominant mutations in the collagen type I encoding genes and recessive defects in proteins involved in collagen type I synthesis and processing and in osteoblast differentiation and activity. Historically, it was believed that the OI bone phenotype was only caused by abnormal collagen type I fibrils in the
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Collagen VI deposition mediates stromal T cell trapping through inhibition of T cell motility in the prostate tumor microenvironment Matrix Biol. (IF 6.9) Pub Date : 2023-06-16 Hawley C. Pruitt, Ya Guan, Hudson Liu, Alexis E Carey, W. Nathaniel Brennen, Jiayun Lu, Corrine Joshu, Ashani Weeraratna, Tamara L. Lotan, T.S. Karin Eisinger-Mathason, Sharon Gerecht
The tumor extracellular matrix (ECM) is a barrier to anti-tumor immunity in solid tumors by disrupting T cell-tumor cell interaction underlying the need for elucidating mechanisms by which specific ECM proteins impact T cell motility and activity within the desmoplastic stroma of solid tumors. Here, we show that Collagen VI (Col VI) deposition correlates with stromal T cell density in human prostate
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Endothelial basement membrane laminins - new players in mouse and human myoendothelial junctions and shear stress communication Matrix Biol. (IF 6.9) Pub Date : 2023-06-11 Anna-Liisa Luik, Melanie-Jane Hannocks, Sophie Loismann, Kishan Kapupara, Manuela Cerina, Miesje van der Stoel, Yaroslav Tsytsyura, Nataliya Glyvuk, Caroline Nordenvall, Jürgen Klingauf, Stephan Huveneers, Sven Meuth, Lars Jakobsson, Lydia Sorokin
Basement membranes (BMs) are critical but frequently ignored components of the vascular system. Using high-resolution confocal imaging of whole-mount-stained mesenteric arteries, we identify integrins, vinculin, focal adhesion kinase (FAK) and several BM proteins including laminins as novel components of myoendothelial junctions (MEJs), anatomical microdomains that are emerging as regulators of cross-talk
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Matrix stiffening facilitates the collective invasion of breast cancer through the periostin-integrin mechanotransduction pathway Matrix Biol. (IF 6.9) Pub Date : 2023-05-23 Tiantian Wu, Shanshan Xiong, Mimi Chen, Bjorn T. Tam, Wei Chen, Ke Dong, Zhenling Ma, Zhe Wang, Gaoliang Ouyang
Matrix rigidity is a critical contributor to tumor progression; however, whether and how matrix stiffness modulates the collective invasion of tumor cells remain unknown. Here we demonstrate that increased matrix stiffness activates YAP to promote the secretion of periostin (POSTN) in cancer-associated fibroblasts, which in turn augments the matrix rigidity of mammary glands and breast tumor tissues
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TGFβ-2 haploinsufficiency causes early death in mice with Marfan syndrome Matrix Biol. (IF 6.9) Pub Date : 2023-05-20 Nalani Sachan, Colin K.L. Phoon, Lior Zilberberg, Matthias C. Kugler, Taylor Ene, Shana B. Mintz, Sae-Il Murtada, Dar Weiss, Glenn I. Fishman, Jay D. Humphrey, Daniel B. Rifkin
To assess the contribution of individual TGF-β isoforms to aortopathy in Marfan syndrome (MFS), we quantified the survival and phenotypes of mice with a combined fibrillin1 (the gene defective in MFS) hypomorphic mutation and a TGF-β1, 2, or 3 heterozygous null mutation. The loss of TGF-β2, and only TGF-β2, resulted in 80% of the double mutant animals dying earlier, by postnatal day 20, than MFS only
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3-O sulfation of syndecan-1 mediated by the sulfotransferase HS3ST3a1 enhances myeloma aggressiveness Matrix Biol. (IF 6.9) Pub Date : 2023-05-16 L. Baert, B. Manfroi, M. Quintero, O. Chavarria, P.V. Barbon, E. Clement, A. Zeller, T. Van Kuppevelt, N. Sturm, J. Moreaux, A. Tveita, B. Bogen, T. McKee, B. Huard
Multiple myeloma is a hematological neoplasm derived from plasma cells invariably developing in the bone marrow (BM). The persisting clinical challenge in MM resides in its high ability to resist drugs as shown by the frequent relapses observed in patients regardless of the treatment applied. In a mouse model of MM, we identified a subpopulation of cells harboring increased resistance to current MM
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Secreted ADAMTS-like 2 promotes myoblast differentiation by potentiating WNT signaling Matrix Biol. (IF 6.9) Pub Date : 2023-05-14 Nandaraj Taye, Mukti Singh, Clair Baldock, Dirk Hubmacher
Myogenesis is the process that generates multinucleated contractile myofibers from muscle stem cells during skeletal muscle development and regeneration. Myogenesis is governed by myogenic regulatory transcription factors, including MYOD1. Here, we identified the secreted matricellular protein ADAMTS-like 2 (ADAMTSL2) as part of a Wnt-dependent positive feedback loop, which augmented or sustained MYOD1
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CaMKII inhibition due to TRIC-B loss-of-function dysregulates SMAD signaling in osteogenesis imperfecta Matrix Biol. (IF 6.9) Pub Date : 2023-05-11 Roberta Besio, Barbara M. Contento, Nadia Garibaldi, Marta Filibian, Stephan Sonntag, Doron Shmerling, Francesca Tonelli, Marco Biggiogera, Marisa Brini, Andrea Salmaso, Milena Jovanovic, Joan C. Marini, Antonio Rossi, Antonella Forlino
Ca2+ is a second messenger that regulates a variety of cellular responses in bone, including osteoblast differentiation. Mutations in trimeric intracellular cation channel B (TRIC-B), an endoplasmic reticulum channel specific for K+, a counter ion for Ca2+flux, affect bone and cause a recessive form of osteogenesis imperfecta (OI) with a still puzzling mechanism. Using a conditional Tmem38b knock out
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Positive feedback loops between fibroblasts and the mechanical environment contribute to dermal fibrosis Matrix Biol. (IF 6.9) Pub Date : 2023-05-08 Liang Zhu, Lechen Liu, Aoli Wang, Jinwen Liu, Xin Huang, Tao Zan
Dermal fibrosis is characterized by excessive deposition of extracellular matrix in the dermis and affects millions of people worldwide and causes limited movement, disfigurement and psychological distress in patients. Fibroblast dysfunction of plays a central role in the pathogenesis of dermal fibrosis and is controlled by distinct factors. Recent studies support the hypothesis that fibroblasts can
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Matricellular proteins in atherosclerosis development Matrix Biol. (IF 6.9) Pub Date : 2023-04-20 Naveed Pervaiz, Ishita Kathuria, Ravi Varma Aithabathula, Bhupesh Singla
The extracellular matrix (ECM) is an intricate network composed of various multi-domain macromolecules like collagen, proteoglycans, and fibronectin, etc., that form a structurally stable composite, contributing to the mechanical properties of tissue. However, matricellular proteins are non-structural, secretory extracellular matrix proteins, which modulate various cellular functions via interacting
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Tumor-Associated Fibrosis Impairs the Response to Immunotherapy Matrix Biol. (IF 6.9) Pub Date : 2023-04-18 Angha Naik, Andrew Leask
Previously, impaired responses to immunotherapy in cancer had been attributed mainly to inherent tumor characteristics (tumor cell intrinsic factors) such as low immunogenicity, (low) mutational burden, weak host immune system, etc. However, mapping the responses of immunotherapeutic regimes in clinical trials for different types of cancer has pointed towards an obvious commonality – that tumors with
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Dual role of enhancer of zeste homolog 2 in the regulation of ultraviolet radiation-induced matrix metalloproteinase-1 and type I procollagen expression in human dermal fibroblasts Matrix Biol. (IF 6.9) Pub Date : 2023-04-07 Min-Kyoung Kim, Hye Sun Shin, Mi Hee Shin, Haesoo Kim, Dong Hun Lee, Jin Ho Chung
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Controlled extracellular proteolysis of thrombospondins Matrix Biol. (IF 6.9) Pub Date : 2023-03-30 Laura Carminati, Elena Carlessi, Elisa Longhi, Giulia Taraboletti
Limited proteolysis of thrombospondins is a powerful mechanism to ensure dynamic tuning of their activities in the extracellular space. Thrombospondins are multifunctional matricellular proteins composed of multiple domains, each with a specific pattern of interactions with cell receptors, matrix components and soluble factors (growth factors, cytokines and proteases), thus with different effects on
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Activation of skeletal muscle FAPs by LPA requires the Hippo signaling via the FAK pathway Matrix Biol. (IF 6.9) Pub Date : 2023-04-03 Meilyn Cruz-Soca, Jennifer Faundez-Contreras, Adriana Córdova-Casanova, Felipe S. Gallardo, Alexia Bock-Pereda, Jerold Chun, Juan Carlos Casar, Enrique Brandan
Lysophosphatidic acid (LPA) is a lysophospholipid that signals through six G-protein coupled receptors (LPARs), LPA1 to LPA6. LPA has been described as a potent modulator of fibrosis in different pathologies. In skeletal muscle, LPA increases fibrosis-related proteins and the number of fibro/adipogenic progenitors (FAPs). FAPs are the primary source of ECM-secreting myofibroblasts in acute and chronic
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Tissue-specific collagen hydroxylation at GEP/GDP triplets mediated by P4HA2 Matrix Biol. (IF 6.9) Pub Date : 2023-03-30 Dafné WILHELM, Alison WURTZ, Hanane ABOUELFARAH, Guillaume SANCHEZ, Catherine BUI, Jean-Baptiste VINCOURT
Collagen, the most abundant organic compound of vertebrate organisms, is a supramolecular, protein-made polymer. Details of its post-translational maturation largely determine the mechanical properties of connective tissues. Its assembly requires massive, heterogeneous prolyl-4-hydroxylation (P4H), catalyzed by Prolyl-4-hydroxylases (P4HA1-3), providing thermostability to its elemental, triple helical
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Health consequences of mutant cartilage oligomeric matrix protein and its relationship to abnormal growth and joint degeneration Matrix Biol. (IF 6.9) Pub Date : 2023-03-30 Jacqueline T. Hecht, Frankie Chiu, Alka Veerisetty, Mohammad Hossain, Karen L. Posey
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Echinatin maintains glutathione homeostasis in vascular smooth muscle cells to protect against matrix remodeling and arterial stiffening Matrix Biol. (IF 6.9) Pub Date : 2023-03-22 Jianrui Zhang, Si-an Xie, Jin Wang, Jiayu Liu, Yueqi Liu, Shuang Zhou, Xixi Li, Lili Han, Wei Pang, Weijuan Yao, Yi Fu, Wei Kong, Min Ye, Jing Zhou
Decreased vascular compliance of the large arteries as indicated by increased pulse wave velocity is shown to be associated with atherosclerosis and the related cardiovascular events. The positive correlation between arterial stiffening and disease progression derives a hypothesis that softening the arterial wall may protect against atherosclerosis, despite that the mechanisms controlling the cellular
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Tonic repression of collagen I by the bradykinin receptor 2 in skin fibroblasts Matrix Biol. (IF 6.9) Pub Date : 2023-03-15 Hui Hui Wong, Sze Hwee Seet, Charles C Bascom, Robert J Isfort, Frederic A Bard
Imbalance of collagen I expression results in severe pathologies. Apart from activation by the TGFβ-receptor/Smad pathway, control of collagen I expression remains poorly understood. Here, we used human dermal fibroblasts expressing a mCherry fluorescent protein driven by endogenous COL1A1 promoter to functionally screen the kinome and phosphatome. We identify 8 negative regulators, revealing that
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Delayed inhibition of collagen deposition by targeting bone morphogenetic protein 1 promotes recovery after spinal cord injury Matrix Biol. (IF 6.9) Pub Date : 2023-03-12 Yifan Huang, Peng Gao, Tao Qin, Bo Chu, Tao Xu, Jiang Yi, Qian Wang, Zhenqi Yang, Tao Jiang, Jin Fan, Shujie Zhao, Wei Zhou, Jian Chen, Guoyong Yin
Fibrotic scars appear after spinal cord injury (SCI) and are mainly composed of fibroblasts and excess extracellular matrix (ECM), including different types of collagen. The temporal and spatial distribution and role of excess collagens and ECM after SCI are not yet fully understood. Here, we identified that the procollagen type I C-terminal propeptide (PICP), a marker of collagen type I deposition
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Conditional expression of endorepellin in the tumor vasculature attenuates breast cancer growth, angiogenesis and hyaluronan deposition Matrix Biol. (IF 6.9) Pub Date : 2023-03-11 Carolyn G. Chen, Aastha Kapoor, Christopher Xie, Alison Moss, Rajanikanth Vadigepalli, Sylvie Ricard-Blum, Renato V. Iozzo
The tumor stroma of most solid malignancies is characterized by a pathological accumulation of pro-angiogenic and pro-tumorigenic hyaluronan driving tumorigenesis and metastatic potential. Of all three hyaluronan synthase isoforms, HAS2 is the primary enzyme that promotes the build-up of tumorigenic HA in breast cancer. Previously, we discovered that endorepellin, the angiostatic C-terminal fragment
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Phase-specific signatures of wound fibroblasts and matrix patterns define cancer-associated fibroblast subtypes Matrix Biol. (IF 6.9) Pub Date : 2023-03-11 Mateusz S. Wietecha, David Lauenstein, Michael Cangkrama, Sybille Seiler, Juyoung Jin, Andreas Goppelt, Manfred Claassen, Mitchell P. Levesque, Reinhard Dummer, Sabine Werner
Healing wounds and cancers present remarkable cellular and molecular parallels, but the specific roles of the healing phases are largely unknown. We developed a bioinformatics pipeline to identify genes and pathways that define distinct phases across the time-course of healing. Their comparison to cancer transcriptomes revealed that a resolution phase wound signature is associated with increased severity
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Dual transgene amelioration of Lama2-null muscular dystrophy Matrix Biol. (IF 6.9) Pub Date : 2023-03-05 Karen K. McKee, Peter D. Yurchenco
Null mutations of the Lama2-gene cause a severe congenital muscular dystrophy and associated neuropathy. In the absence of laminin-α2 (Lmα2) there is a compensatory replacement by Lmα4, a subunit that lacks the polymerization and α-dystroglycan (αDG)-binding properties of Lmα2. The dystrophic phenotype in the dy3K/dy3K Lama2−/− mouse were evaluated with transgenes driving expression of two synthetic
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Increased expression of the pathological O-glycosylated form of oncofetal fibronectin in the multidrug resistance phenotype of cancer cells Matrix Biol. (IF 6.9) Pub Date : 2023-03-05 Jhenifer Santos dos Reis, Marcos André Rodrigues da Costa Santos, Kelli Monteiro da Costa, Celio Geraldo Freire-de-Lima, Alexandre Morrot, Jose Osvaldo Previato, Lucia Mendonça Previato, Leonardo Marques da Fonseca, Leonardo Freire-de-Lima
Changes in protein glycosylation are a hallmark of transformed cells and modulate numerous phenomena associated with cancer progression, such as the acquisition of multidrug resistance (MDR) phenotype. Different families of glycosyltransferases and their products have already been described as possible modulators of the MDR phenotype. Among the glycosyltransferases intensively studied in cancer research
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CCBE1 promotes mitochondrial fusion by inhibiting the TGFβ-DRP1 axis to prevent the progression of hepatocellular carcinoma Matrix Biol. (IF 6.9) Pub Date : 2023-02-26 Guang-Ang Tian, Wen-Ting Xu, Xue-Li Zhang, Yao-Qi Zhou, Yue Sun, Li-Peng Hu, Shu-Heng Jiang, Hui-Zhen Nie, Zhi-Gang Zhang, Lei Zhu, Jun Li, Xiao-Mei Yang, Lin-Li Yao
The extracellular matrix (ECM), as an important component of the tumor microenvironment, exerts various roles in tumor formation. Mitochondrial dynamic disorder is closely implicated in tumorigenesis, including hyperfission in HCC. We aimed to determine the influence of the ECM-related protein CCBE1 on mitochondrial dynamics in HCC. Here, we found that CCBE1 was capable of promoting mitochondrial fusion
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Different molecular weights of hyaluronan research in knee osteoarthritis: A state-of-the-art review Matrix Biol. (IF 6.9) Pub Date : 2023-02-26 Ko-Hsiu Lu, Peace Wun-Ang Lu, Chiao-Wen Lin, Eric Wun-Hao Lu, Shun-Fa Yang
Osteoarthritis (OA), the most common form of arthritis, is characterized by progressive cartilage destruction, concomitant adaptive osteogenesis, and loss of joint function. The progression of OA with aging is associated with a decrease in native hyaluronan (HA, hyaluronate or hyaluronic acid) with a high molecular weight (HMW) in synovial fluid and a subsequent increase in lower MW HA and fragments
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Targeting LAYN inhibits colorectal cancer metastasis and tumor-associated macrophage infiltration induced by hyaluronan oligosaccharides Matrix Biol. (IF 6.9) Pub Date : 2023-02-18 Yabing Yang, Zuyang Chen, Xiaodong Chu, Qiuxia Yan, Jiashuai He, Yanguan Guo, Zhan Zhao, Yiran Zhang, Dahai Hu, Hui Ding, Xiaoxu Zhao, Yunlong Pan, Hongmei Dong, Lu Wang, Jinghua Pan
The accumulation of hyaluronan oligosaccharides (oHA) in colorectal cancer (CRC) is closely related to tumor metastasis, but the underlying mechanism remains unclear. In this study, we first described that LAYN, a novel HA receptor, was upregulated in CRC tissue. Aberrant LAYN expression correlated with CRC metastasis and poor prognosis and positively correlated with tumor-associated macrophage (TAM)
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Extracellular matrix in high-grade serous ovarian cancer: Advances in understanding of carcinogenesis and cancer biology Matrix Biol. (IF 6.9) Pub Date : 2023-02-11 Yazmin Brown, Susan Hua, Pradeep S. Tanwar
High-grade serous ovarian cancer (HGSOC) is notoriously known as the “silent killer” of post-menopausal women as it has an insidious progression and is the deadliest gynaecological cancer. Although a dual origin of HGSOC is now widely accepted, there is growing evidence that most cases of HGSOC originate from the fallopian tube epithelium. In this review, we will address the fallopian tube origin and
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Hyaluronan in the pathogenesis of acute and post-acute COVID-19 infection Matrix Biol. (IF 6.9) Pub Date : 2023-02-05 Henry W. Barnes, Sally Demirdjian, Naomi L. Haddock, Gernot Kaber, Hunter A. Martinez, Nadine Nagy, Harry Karmouty-Quintana, Paul L. Bollyky
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) recently emerged as the cause of a global pandemic. Infection with SARS-CoV-2 can result in COVID-19 with both acute and chronic disease manifestations that continue to impact many patients long after the resolution of viral replication. There is therefore great interest in understanding the host factors that contribute to COVID-19 pathogenesis
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A new mouse model of elastin haploinsufficiency highlights the importance of elastin to vascular development and blood pressure regulation Matrix Biol. (IF 6.9) Pub Date : 2023-02-10 Bridget M. Brengle, Michelle Lin, Robyn A. Roth, Kara D. Jones, Jessica E. Wagenseil, Robert P. Mecham, Carmen M. Halabi
Supravalvular aortic stenosis (SVAS) is an autosomal dominant disease resulting from elastin (ELN) haploinsufficiency. Individuals with SVAS typically develop a thickened arterial media with an increased number of elastic lamellae and smooth muscle cell (SMC) layers and stenosis superior to the aortic valve. A mouse model of SVAS (Eln+/−) was generated that recapitulates many aspects of the human disease
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Low molecular weight hyaluronan inhibits lung epithelial ion channels by activating the calcium-sensing receptor Matrix Biol. (IF 6.9) Pub Date : 2023-02-08 Ahmed Lazrak, Weifeng Song, Zhihong Yu, Shaoyan Zhang, Anoma Nellore, Charles W. Hoopes, Bradford A. Woodworth, Sadis Matalon
Herein, we tested the hypothesis that low molecular weight hyaluronan (LMW-HA) inhibits lung epithelial ions transport in-vivo, ex-vivo, and in-vitro by activating the calcium-sensing receptor (CaSR). Twenty-four hours post intranasal instillation of 50–150 µg/ml LMW-HA to C57BL/6 mice, there was a 75% inhibition of alveolar fluid clearance (AFC), a threefold increase in the epithelial lining fluid
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Mechanical loading is required for initiation of extracellular matrix deposition at the developing murine myotendinous junction Matrix Biol. (IF 6.9) Pub Date : 2023-01-26 Sarah N. Lipp, Kathryn R. Jacobson, Haley A. Colling, Tyler G. Tuttle, Dalton T. Miles, Kaitlin P. McCreery, Sarah Calve
The myotendinous junction (MTJ) contributes to the generation of motion by connecting muscle to tendon. At the adult MTJ, a specialized extracellular matrix (ECM) is thought to contribute to the mechanical integrity of the muscle-tendon interface, but the factors that influence MTJ formation during mammalian development are unclear. Here, we combined 3D imaging and proteomics with murine models in
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Infiltrating CD8+ T cells and M2 macrophages are retained in tumor matrix tracks enriched in low tension fibronectin fibers Matrix Biol. (IF 6.9) Pub Date : 2023-01-18 Charlotte M. Fonta, Thomas Loustau, Chengbei Li, Suchithra Poilil Surendran, Uwe Hansen, Devadarssen Murdamoothoo, Mario C. Benn, Ines Velazquez-Quesada, Raphael Carapito, Gertraud Orend, Viola Vogel
Tracks rich in matrix and cells, as described in several cancer types, have immunosuppressive functions and separate tumor nests and stroma, yet their origin is unknown. Immunostainings of cryosections from mouse breast tumors show that these tracks are bordered by an endothelial-like basement membrane, filled with fibers of collagen adjacent to tenascin-C (TNC) and low-tension fibronectin (Fn) fibers
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Temporally and spatially regulated collagen XVIII isoforms are involved in ureteric tree development via the TSP1-like domain Matrix Biol. (IF 6.9) Pub Date : 2023-01-06 Mia M. Rinta-Jaskari, Florence Naillat, Heli J. Ruotsalainen, Jarkko T. Koivunen, Takako Sasaki, Ilkka Pietilä, Harri P. Elamaa, Inderjeet Kaur, Aki Manninen, Seppo J. Vainio, Taina A. Pihlajaniemi
Collagen XVIII (ColXVIII) is a component of the extracellular matrix implicated in embryogenesis and control of tissue homoeostasis. We now provide evidence that ColXVIII has a specific role in renal branching morphogenesis as observed in analyses of total and isoform-specific knockout embryos and mice. The expression of the short and the two longer isoforms differ temporally and spatially during renal
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Lamellar thickness measurements in control and osteogenesis imperfecta human bone, with development of a method of automated thickness averaging to simplify quantitation Matrix Biol. (IF 6.9) Pub Date : 2022-12-31 Jarred Chow, Nicolas Ryan, Sandra J. Shefelbine, Frederic Shapiro
Lamellar bone that forms in moderate and severe osteogenesis imperfecta (OI) is composed of structurally irregular lamellae compared to those in control bone. OI and control cortical bone fragments were prepared for light microscopy in standardized fashion: decalcified, embedded in plastic, sectioned and stained with toluidine blue. Polarization light microscopy (PLM) was used to demonstrate and quantify
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Decorin improves human pancreatic β-cell function and regulates ECM expression in vitro Matrix Biol. (IF 6.9) Pub Date : 2022-12-30 Max Urbanczyk, Abiramy Jeyagaran, Aline Zbinden, Chuan-en Lu, Julia Marzi, Laurence Kuhlburger, Sven Nahnsen, Shannon L. Layland, Garry Duffy, Katja Schenke-Layland
Transplantation of islets of Langerhans is a promising alternative treatment strategy in severe cases of type 1 diabetes mellitus; however, the success rate is limited by the survival rate of the cells post-transplantation. Restoration of the native pancreatic niche during transplantation potentially can help to improve cell viability and function. Here, we assessed for the first time the regulatory
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Substrate stiffness regulates the recurrent glioblastoma cell morphology and aggressiveness Matrix Biol. (IF 6.9) Pub Date : 2022-12-21 Anagha Acharekar, Ketaki Bachal, Pallavi Shirke, Rahul Thorat, Archisman Banerjee, Nilesh Gardi, Abhijit Majumder, Shilpee Dutt
Recurrent glioblastoma is highly aggressive with currently no specific treatment regime. Therefore, to identify novel therapeutic targets for recurrent GBM, we used a cellular model developed in our lab from commercially available cell line U87MG and patient-derived cultures that allows the comparison between radiation naïve (Parent) and recurrent GBM cells generated after parent cells are exposed