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Targeting ceramide synthases for the development of new antifungals Structure (IF 4.3) Pub Date : 2025-06-23 Deveney Dasilva, Nivea Pereira de Sa, Kathryn Takemura, Kalani Jayanetti, Jeehyun Karen You, Nathalia Vieira de Sa, Gabriel Soares Matos, Andy Zhong, Can E. Senkal, Yusuf Hannun, Iwao Ojima, John Mallamo, John B. McCarthy, Maurizio Del Poeta
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Rapid hepatitis C virus replication machinery removal after antiviral treatment with DAA monitored by multimodal imaging Structure (IF 4.4) Pub Date : 2025-06-18 Victoria Castro, Gema Calvo, Ana J. Pérez-Berná, Kevin Mamprin, Sergey Kapishnikov, David Rogers, Stephen O'Connor, Paul Sheridan, Kenneth Fahy, Eva Pereiro, Pablo Gastaminza
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High throughput cryo-EM provides structural understanding for modulators of the lysosomal ion channel TRPML1 Structure (IF 4.4) Pub Date : 2025-06-17 Judith Reeks, Pravin Mahajan, Mellissa Clark, Suzanna R. Cowan, Elena Di Daniel, Christopher P. Earl, Samantha Fisher, Rhian S. Holvey, Scott M. Jackson, Emyr Lloyd-Evans, Carmine M. Morgillo, Paul N. Mortenson, Marc O’Reilly, Caroline J. Richardson, Patrick Schöpf, Daniel M. Tams, Helen Waller-Evans, Simon E. Ward, Stuart Whibley, Pamela A. Williams, Christopher N. Johnson
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The continuous evolution of biomolecular force fields Structure (IF 4.4) Pub Date : 2025-06-16 Xiaoli Lu, Jinfeng Chen, Jing Huang
Biomolecular force fields have continuously evolved to improve their accuracy and broaden their applications in biological and therapeutic discoveries. The rapid adaptation of advanced computational technology, in particular the recent deep learning revolution, has led to an unprecedented ability to model and simulate biomolecules, as well as new opportunities in force field parametrization. Here,
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Redefining the role of the EryM acetyltransferase in natural product biosynthetic pathways Structure (IF 4.4) Pub Date : 2025-06-13 Yihua Li, Xunkun Liu, Natalia R. Harris, Jacquelyn R. Roberts, Estefanía Martínez Valdivia, Xinrui Ji, Janet L. Smith
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A class III ligand oscillates between internal and terminal binding modes as it engages with the Dishevelled PDZ domain Structure (IF 4.4) Pub Date : 2025-06-13 Jitender Kumar, Miroslav Micka, Jan Komárek, Tomáš Klumpler, Vojtěch Bystrý, Remco Sprangers, Cyril Bařinka, Vítězslav Bryja, Konstantinos Tripsianes
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Structural insights into ZBTB20 action at the AFP promoter Structure (IF 4.4) Pub Date : 2025-06-09 Lingna Yang, Huajiao Xie, Xiaoqing Wan, Mengfeng Li, Mengqi Lv, Yi Duan, Yunyu Shi, Weiping J. Zhang, Fudong Li
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Secondary chelation through shared water provides ion selectivity in bacterial sodium channels Structure (IF 4.4) Pub Date : 2025-06-09 Yury A. Trofimov, Anton O. Chugunov, Alexander A. Vassilevski
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Antimalarial drug artemisinin stabilizes PfRACK1 binding to the ribosome Structure (IF 4.4) Pub Date : 2025-06-05 Ka Diam Go, Xin-Fu Yan, Grennady Wirjanata, Rya Ero, Samuel Pazicky, Jerzy Dziekan, Seth Tjia, Julien Lescar, Zbynek Bozdech, Yong-Gui Gao
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Structural insights into functional regulation of the human CPEB3 prion by an amyloid-forming segment Structure (IF 4.4) Pub Date : 2025-06-05 Maria D. Flores, Michael R. Sawaya, David R. Boyer, Samantha Zink, Susanna Tovmasyan, Adrian Saucedo, Logan S. Richards, Chih-Te Zee, Jorge Cardenas, Luana Fioriti, Jose A. Rodriguez
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The effect of G-quadruplexes on TDP43 condensation, distribution, and toxicity Structure (IF 4.4) Pub Date : 2025-06-05 Emily G. Oldani, Kevin M. Reynolds Caicedo, McKenna E. Spaeth Herda, Adam H. Sachs, Erich G. Chapman, Sunil Kumar, Daniel A. Linseman, Scott Horowitz
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Structural plasticity of bacterial ubiquitin-like proteins in stress sensing Structure (IF 4.3) Pub Date : 2025-06-05 Weixun Li, Xiang Gao
In this issue of Structure, Gong et al.1 provide structural insights into the diversity of bacterial ubiquitin-like proteins and characterize their Ca2+-dependent oligomerization into dimers and filaments. Their findings suggest a potential mechanism by which fluctuations in metal-ion concentrations could modulate bacterial stress-response pathways.
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Color mapping of multitarget protein clusters Structure (IF 4.3) Pub Date : 2025-06-05 Hirushi Gunasekara, Ying S. Hu
Visualizing subcellular distributions of proteins and assessing their colocalization patterns are central to understanding the organization and interactions of molecular assemblies. In this issue of Structure, Kiuchi et al.1 introduce protein cluster coloring to map complex association patterns among eight endogenous proteins at the periphery of the clathrin-coated structure, revealing their multilayered
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Need for speed: Mass photometry as a sample analysis tool for structural studies Structure (IF 4.3) Pub Date : 2025-06-05 Lauren E. Vostal
Mass photometry (MP) is a label-free approach that measures the mass of single molecules in solution. Here, we discuss the principles of MP, example uses of the technique, and how it can be a valuable tool for structural biologists in streamlining cryoelectron microscopy (cryo-EM) pipelines.
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Biochemical analysis of PD-L1 ubiquitination by CRL3SPOP, ARIH1, and NEDD4 family ubiquitin ligases Structure (IF 4.4) Pub Date : 2025-06-04 Guojiao Xie, Lin Gao, Renee Lu, Linxia Tian, Tiantian Zheng, Xinning Li, Yongjun Dang, Philip A. Cole, Xian Yu, Hanjie Jiang, Zan Chen
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Structural insights into the interaction of Hir2 and Hpc2 in the yeast Hir histone chaperone complex Structure (IF 4.4) Pub Date : 2025-05-30 Chu-Hsin Tseng, Wen-Lin Hsieh, Wesley Tien Chiang, Nien-Jen Hu, Chia-Liang Lin
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The MIT domain of STAMBP autoinhibits its deubiquitination activity Structure (IF 4.4) Pub Date : 2025-05-28 Ziyue Chen, Guanchao Wang, Yifan Zhang, Jianping Ding
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Exploring insulin-receptor dynamics: Stability and binding mechanisms Structure (IF 4.4) Pub Date : 2025-05-26 Amanda D. Stange, Lorena Zuzic, Birgit Schiøtt, Nils A. Berglund
Insulin binding to the insulin receptor (IR) induces large conformational changes leading to receptor activation. Although there exists a considerable number of IR structures in different conformational and insulin-saturation states, they cannot provide dynamic information or the resolved order of events leading to receptor activation. In this study, we employed molecular dynamics (MD) simulations
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Acquisition of quaternary trimer interaction as a key step in the lineage maturation of a broad and potent HIV-1 neutralizing antibody Structure (IF 4.4) Pub Date : 2025-05-23 Qingbo Liu, Ruth J. Parsons, Kevin Wiehe, Robert J. Edwards, Kevin O. Saunders, Peng Zhang, Huiyi Miao, Kedamawit Tilahun, Julia Jones, Yue Chen, Bhavna Hora, Wilton B. Williams, David Easterhoff, Xiao Huang, Katarzyna Janowska, Katayoun Mansouri, Barton F. Haynes, Priyamvada Acharya, Paolo Lusso
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Unprocessed BMP9 precursor is an intrinsic antagonist for its active growth factor Structure (IF 4.3) Pub Date : 2025-05-23 Weida Zhang, Yuanyuan Zhang, Weidong Mao, Tao Huang, Xinrong Yu, Xiaohong Qin, Li-Zhi Mi
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Chloride binding does not influence prestin motor speed at very high frequencies in the mouse outer hair cell Structure (IF 4.4) Pub Date : 2025-05-21 Jun-Ping Bai, Chenou Zhang, Iman Bahader, Nicola Strenzke, Vijay Renigunta, Dominik Oliver, Dhasakumar Navaratnam, Oliver Beckstein, Joseph Santos-Sacchi
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The molecular mechanisms of visual chromophore release from cellular retinaldehyde-binding protein Structure (IF 4.4) Pub Date : 2025-05-21 Daniel Santos, Lorenzo Foglia, Philip D. Kiser, Alvin Yu
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Structural study on human microbiome-derived polyketide synthases that assemble genotoxic colibactin Structure (IF 4.4) Pub Date : 2025-05-16 Minjae Kim, Jinwoo Kim, Gyu Sung Lee, Paul Dominic B. Olinares, Yougant Airan, Jasmine L. Chow, Jongseok Park, Yujin Jeong, Jiho Park, Brian T. Chait, Seth B. Herzon, Chung Sub Kim, Jin Young Kang
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A comprehensive engineering strategy improves potency and manufacturability of a near pan-neutralizing antibody against HIV Structure (IF 4.4) Pub Date : 2025-05-14 Mohammad M. Sajadi, Abdolrahim Abbasi, Zahra Rikhtegaran Tehrani, Christine Siska, Rutilio Clark, Woo Chi, Michael S. Seaman, Dieter Mielke, Kshitij Wagh, Qingbo Liu, Taylor Jumpa, Randal R. Ketchem, Dung N. Nguyen, William D. Tolbert, Brian G. Pierce, Ben Atkinson, Derrick Deming, Megan Sprague, Andrew Asakawa, David Ferrer, Anthony DeVico
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Structural insights from active site variants and β-8 loop interactions in viperin-like enzymes Structure (IF 4.4) Pub Date : 2025-05-14 Jake C. Lachowicz, Steven Grudman, Jeffrey B. Bonanno, Andras Fiser, Tyler L. Grove
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Structural and functional interrelationships of histone H2A with its variants H2A.Z and H2A.W in Arabidopsis Structure (IF 4.4) Pub Date : 2025-05-12 Youchao Wang, Jiabing Wu, Shuoming Yang, Xiang Li, Jiachen Wang, Qinghe Lv, Xiaoyu Zhu, Guoliang Lu, Jinru Zhang, Wen-Hui Shen, Bing Liu, Jinzhong Lin, Aiwu Dong
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Sla2 is a core interaction hub for clathrin light chain and the Pan1/End3/Sla1 complex Structure (IF 4.4) Pub Date : 2025-05-09 George Draper-Barr, Lucas A. Defelipe, David Ruiz-Carrillo, Emil Gustavsson, Meytal Landau, Maria García-Alai
The interaction network of Sla2, a vital endocytic mid-coat adaptor protein, undergoes constant rearrangement. Sla2 serves as a scaffold linking the membrane to the actin cytoskeleton, with its role modulated by the clathrin light chain (CLC), which inhibits Sla2’s function under certain conditions. We show that Sla2 has two independent binding sites for CLC: one previously described in homologs of
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Structural insights into the human system y+L amino acid transporter complex Structure (IF 4.4) Pub Date : 2025-05-09 Lu Dai, Kangtai Xu, Ting Zhang, Xiaoting Wang, Qian Zeng, Hao Liang, Chenye Xu, Liuqing Yang, Zilong Wang, Renhong Yan
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Structural basis of human uromodulin filament networks in uropathogen capture Structure (IF 4.4) Pub Date : 2025-05-08 Andrew N. Chang, Gabriele Cerutti, Yuki Ogawa, Alia Basler, Willa Switzer, Mia Eng-Kohn, Carolyn Lee, Anthony W.P. Fitzpatrick
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The YEATS domain is a selective reader of histone methacrylation Structure (IF 4.4) Pub Date : 2025-05-07 Dustin C. Becht, Jiabao Song, Karthik Selvam, Kejun Yin, Weizhi Bai, Yingming Zhao, Ronghu Wu, Y. George Zheng, Tatiana G. Kutateladze
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Asymmetric structure of podophage N4 from the Schitoviridae family reveals a type of tube-sheath short-tail architecture Structure (IF 4.4) Pub Date : 2025-05-02 Jing Zheng, Hao Pang, Hao Xiao, Junquan Zhou, Zhonghua Liu, Wenyuan Chen, Hongrong Liu
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Introducing Bonnie Murphy, Ville Kaila, Maxie Roessler, Volha Chukhutsina, Alisia Fadini, Sonya Hanson, Filipe Maia, and Kirill Kovalev Structure (IF 4.4) Pub Date : 2025-05-01 Bonnie J. Murphy, Ville R.I. Kaila, Maxie M. Roessler, Volha Chukhutsina, Alisia Fadini, Sonya M. Hanson, Filipe R.N.C. Maia, Kirill Kovalev
In this Voices article, we introduce eight impressive scientists at different career stages who spoke at the recent “8th Ringberg Workshop on Structural Biology (with FELs)” organized by Ilme Schlichting from the Max Planck Institute for Medical Research, Heidelberg. We asked the authors to tell us more about themselves and their exciting research.
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Cryo-ET unravels the mystery of Ad5-nCoV vaccines Structure (IF 4.4) Pub Date : 2025-05-01 Chang Liu, Zunlong Ke
The Ad5-nCoV vaccine (Convidecia) against COVID-19 showed promising clinical results. However, the molecular mechanisms underlying its high immunogenicity and potential adverse reactions have remained elusive. In this issue of Structure, Dong et al.1 employed cryo-electron tomography as a powerful technique to show that abundant prefusion spike protein formation is induced by Ad5-nCoV vaccines.
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Putting the brakes on endocytosis Structure (IF 4.4) Pub Date : 2025-05-01 Bernard T. Kelly
In this issue of Structure, Horatscheck et al.1 report the isolation and characterization of two improved small molecule drugs for the selective inhibition of clathrin-mediated endocytosis.
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Functional conformations or poor mimetics? Structure (IF 4.4) Pub Date : 2025-05-01 Hugues Nury, Rouslan G. Efremov
Membrane proteins function within lipid membranes but require mimetics for structural studies. In this issue of Structure, Hoffmann et al.1 show how different mimetics bias the conformational spectrum of the MsbA transporter. This study highlights the need for improved membrane mimetics and sustained efforts to link conformations to function.
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Structural insights into the receptor-binding domain of bat coronavirus ZXC21 Structure (IF 4.4) Pub Date : 2025-04-29 Chenghai Wang, Xiaoyan Nan, Yang Deng, Shilong Fan, Xin Li, Jun Lan
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A distinctive IGHV3-66 SARS-CoV-2 neutralizing antibody elicited by primary infection with an Omicron variant Structure (IF 4.4) Pub Date : 2025-04-29 Qing Fan, Congcong Liu, Huimin Guo, Shilong Tang, Haiyan Wang, Bing Zhou, Yuehong Sun, Miao Wang, Xiangyang Ge, Lei Liu, Bin Ju, Zheng Zhang
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Cholesterol-dependent dimerization and conformational dynamics of EphA2 receptors from coarse-grained and all-atom simulations Structure (IF 4.4) Pub Date : 2025-04-24 Amita Rani Sahoo, Nisha Bhattarai, Matthias Buck
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Laminar organization of molecular complexes in a clathrin coat revealed by nanoscale protein colocalization Structure (IF 4.3) Pub Date : 2025-04-23 Tai Kiuchi, Ryouhei Kobayashi, Shuichiro Ogawa, Louis L.H. Elverston, Dimitrios Vavylonis, Naoki Watanabe
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Cryo-EM structures of a Xanthomonas phage: Insights into viral architecture and implications for the model phage HK97 Structure (IF 4.3) Pub Date : 2025-04-23 Mingcheng Guo, Aohan Wang, Yaqi Zheng, Chaoying Liu, Qianqian Shao, Yunfei Deng, Lin Li, Yueting Wang, Xiaofang Wang, Yue Shen, Jun Qian, Xiaofeng Zhou, Qianglin Fang
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The structure of the R2T complex reveals a different architecture from the related HSP90 cochaperone R2TP Structure (IF 4.4) Pub Date : 2025-04-18 Alberto Palacios-Abella, Andrés López-Perrote, Jasminka Boskovic, Sandra Fonseca, Cristina Úrbez, Vicente Rubio, Oscar Llorca, David Alabadí
(Structure 33, 740–752; April 3, 2025)
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Structural diversity and oligomerization of bacterial ubiquitin-like proteins Structure (IF 4.3) Pub Date : 2025-04-17 Minheng Gong, Qiaozhen Ye, Yajie Gu, Lydia R. Chambers, Andrey A. Bobkov, Neal K. Arakawa, Mariusz Matyszewski, Kevin D. Corbett
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Structural insights into the assembly and regulation of 2′-O RNA methylation by SARS-CoV-2 nsp16/nsp10 Structure (IF 4.3) Pub Date : 2025-04-11 Anurag Misra, R. Rahisuddin, Manish Parihar, Shailee Arya, Thiruselvam Viswanathan, Nathaniel Jackson, Shan Qi, Siu-Hong Chan, Reuben S. Harris, Luis Martinez-Sobrido, Yogesh K. Gupta
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High-resolution cryo-EM analysis visualizes hydrated type I and IV pilus structures from enterotoxigenic Escherichia coli Structure (IF 4.3) Pub Date : 2025-04-11 Kazuki Kawahara, Hiroya Oki, Minato Iimori, Ryuki Muramoto, Tomoya Imai, Christoph Gerle, Hideki Shigematsu, Shigeaki Matsuda, Tetsuya Iida, Shota Nakamura
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RQC2 is a major player in peptide release from stalled ribosomes Structure (IF 4.3) Pub Date : 2025-04-04 Céline Fabret, Emmanuel Giudice, Sophie Chat, Reynald Gillet, Olivier Namy
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A tale of detergent tails: GPCR activation beyond ligands Structure (IF 4.4) Pub Date : 2025-04-03 Eugene Agyemang, Rajan Lamichhane
In this issue of Structure, Banerjee et al.1 use single-molecule FRET to explore how various detergents and cholesterol influence the conformational dynamics of the extracellular domain of the metabotropic glutamate receptor 2 (mGluR2). They show that the local membrane environment modulates the receptor’s active-inactive state equilibrium and identifies specific cholesterol-binding sites, offering
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Spy-ing on nucleic acids: Atomic resolution of the S. pyogenes CRISPR-Cas9 surveillance state Structure (IF 4.4) Pub Date : 2025-04-03 Alexa L. Knight, George P. Lisi
In a recent issue of Cell Chemical Biology, De Paula et al.1 report an extensive methyl-TROSY solution NMR study of the CRISPR-Cas9 holoenzyme. Studying millisecond-to-second protein dynamics using individual domain constructs of Cas9 coupled to structural interrogations of the full-length enzyme, the authors describe the Cas9 “surveillance state,” a molecular mechanism driving the discrimination between
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Structure of the Staphylococcus aureus bacteriophage 80α neck shows details of the DNA, tail completion protein, and tape measure protein Structure (IF 4.3) Pub Date : 2025-04-01 James L. Kizziah, Amarshi Mukherjee, Laura K. Parker, Terje Dokland
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Disulfide-stabilized diabodies enable near-atomic cryo-EM imaging of small proteins: A case study of the bacterial Na+/citrate symporter CitS Structure (IF 4.3) Pub Date : 2025-03-31 Subin Kim, Ji Won Kim, Jun Gyou Park, Sang Soo Lee, Seung Hun Choi, Jie-Oh Lee, Mi Sun Jin
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Structural basis for the constitutive activity of the melanocortin receptor family Structure (IF 4.3) Pub Date : 2025-03-28 Wenbo Feng, Qingtong Zhou, Chang Zheng, Dehua Yang, Ming-Wei Wang
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ATP binding controls the molecular function of bacterial MutS2 by mediating closure of the dimeric clamp structure Structure (IF 4.3) Pub Date : 2025-03-28 Kenji Fukui, Takeshi Murakawa, Nobumasa Hino, Naoyuki Kondo, Takato Yano
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A mesophilic Argonaute from Clostridium formicaceticum with efficient DNA cleavage activity guided by small DNA Structure (IF 4.3) Pub Date : 2025-03-28 Jianrui Li, Meixia Yu, Zhijia Yang, Yueheng Zhou, Yunpeng Teng, Zijian Wang, Jian Chen, Jinsheng Lai, Beibei Xin
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Structurally diverse C-terminal accessory domains in type I ABC importers reveal distinct regulatory mechanisms Structure (IF 4.4) Pub Date : 2025-03-24 Evan R. Buechel, Valentina S. Dimitrova, Alexandra Karagiaridi, Lydia G. Kenney, Heather W. Pinkett
ATP-binding cassette (ABC) transporters are critical for cellular processes, facilitating the transport of various substrates across membranes by harnessing ATP hydrolysis. These transporters are divided into importers and exporters, with importers playing key roles in nutrient uptake and bacterial virulence. Despite their therapeutic potential as drug targets, the regulatory mechanisms governing ABC
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A structural perspective on the temperature dependent activity of enzymes Structure (IF 4.4) Pub Date : 2025-03-21 Matthew J. McLeod, Sarah A.E. Barwell, Todd Holyoak, Robert E. Thorne
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Molecular basis of Ad5-nCoV vaccine-induced immunogenicity Structure (IF 4.4) Pub Date : 2025-03-19 Dongyang Dong, Yutong Song, Shipo Wu, Busen Wang, Cheng Peng, Weiping Zhang, Weizheng Kong, Zheyuan Zhang, Jingwen Song, Li-Hua Hou, Sai Li
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Distinct binding modes drive the broad neutralization profile of two persistent influenza hemagglutinin stem-specific antibody lineages Structure (IF 4.4) Pub Date : 2025-03-19 Grace E. Mantus, Gabriele Cerutti, Michael Chambers, Rebecca A. Gillespie, Geoffrey D. Shimberg, Abby Spangler, Jason Gorman, Tongqing Zhou, Chen-Hsiang Shen, Masaru Kanekiyo, Peter D. Kwong, Lawrence Shapiro, Sarah F. Andrews
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Cryo-EM structure of the human monocarboxylate transporter 10 Structure (IF 4.4) Pub Date : 2025-03-19 Viktoria Bågenholm, Karl Patric Nordlin, Andrea Pasquadibisceglie, Andrey Belinskiy, Caroline Marcher Holm, Hajira Ahmed Hotiana, Kamil Gotfryd, Lucie Delemotte, Hussam Hassan Nour-Eldin, Per Amstrup Pedersen, Pontus Gourdon
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Next-generation small molecule inhibitors of clathrin function acutely inhibit endocytosis Structure (IF 4.4) Pub Date : 2025-03-19 André Horatscheck, Michael Krauß, Haydar Bulut, Valerie Chambon, Massilullah Shafaq Zadah, Estelle Dransart, Kimberly Peloza, Karine F. Santos, Mark J. Robertson, Kate Prichard, Sandra Miksche, Silke Radetzki, Jens-Peter von Kries, Markus C. Wahl, Adam McCluskey, Ludger Johannes, Volker Haucke, Marc Nazaré
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Structure and dynamics of a nucleosome core particle based on Widom 603 DNA sequence Structure (IF 4.4) Pub Date : 2025-03-17 Grigoriy A. Armeev, Andrey V. Moiseenko, Nikita A. Motorin, Dmitriy A. Afonin, Lei Zhao, Veniamin A. Vasilev, Pavel D. Oleinikov, Grigory S. Glukhov, Georgy S. Peters, Vasily M. Studitsky, Alexey V. Feofanov, Alexey K. Shaytan, Xiangyan Shi, Olga S. Sokolova
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Structures and mechanisms of the ABC transporter ABCB1 from Arabidopsis thaliana Structure (IF 4.4) Pub Date : 2025-03-17 Qian Chen, Li Zhu, Sufen Zhang, Shuai Qiao, Zhong Jie Ding, Shao Jian Zheng, Jiangtao Guo, Nannan Su