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FakET: Simulating cryo-electron tomograms with neural style transfer Structure (IF 4.4) Pub Date : 2025-02-12 Pavol Harar, Lukas Herrmann, Philipp Grohs, David Haselbach
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Dynamic integration of feature- and template-based methods improves the prediction of conformational B cell epitopes Structure (IF 4.4) Pub Date : 2025-02-11 Yueyue Shen, Zheng Jiang, Rong Liu
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Unique mechanisms to increase structural stability and enhance antigen binding in nanobodies Structure (IF 4.4) Pub Date : 2025-02-11 Natalia E. Ketaren, Peter C. Fridy, Vladimir Malashkevich, Tanmoy Sanyal, Marc Brillantes, Mary K. Thompson, Deena A. Oren, Jeffrey B. Bonanno, Andrej Šali, Steven C. Almo, Brian T. Chait, Michael P. Rout
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Selective deuteration of an RNA:RNA complex for structural analysis using small-angle scattering Structure (IF 4.4) Pub Date : 2025-02-10 Aldrex Munsayac, Wellington C. Leite, Jesse B. Hopkins, Ian Hall, Hugh M. O’Neill, Sarah C. Keane
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CryoVIA: An image analysis toolkit for the quantification of membrane structures from cryo-EM micrographs Structure (IF 4.4) Pub Date : 2025-02-06 Philipp Schönnenbeck, Benedikt Junglas, Carsten Sachse
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The mechanosensitive channel YbiO has a conductance equivalent to the largest gated-pore Structure (IF 4.4) Pub Date : 2025-02-06 Benjamin J. Lane, Mariangela Dionysopoulou, Nana Yan, Jonathan D. Lippiat, Stephen P. Muench, Christos Pliotas
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The Anti-TB Structural Center at ShanghaiTech University Structure (IF 4.4) Pub Date : 2025-02-06 Haitao Yang, Lu Zhang, Quan Wang, Jun Li, Xiuna Yang, Yan Gao, Bing Zhang
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Transcription termination—Some like it hot Structure (IF 4.4) Pub Date : 2025-02-06 Kristine Bourke Arnvig, Finn Werner
In this issue of Structure, Dikunova et al.1 report the structure of the trimeric torpedo complex, the key factor responsible for transcription termination by RNA polymerase II R(NAPII) at the end of protein-encoding genes. The comparison between meso- and thermophilic torpedoes provides intriguing insights into thermal adaptions and mechanisms of termination.
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Epithelial sodium channels assemble in an orderly manner: Biology does not play dice Structure (IF 4.4) Pub Date : 2025-02-06 Sarah Pellizzari, Erhu Cao
In this issue of Structure, Houser et al.1 report cryoelectron microscopy (cryo-EM) structures of two atypical forms of the heterotrimeric epithelial sodium channel (ENaC) in which either a δ or a β subunit assembles with one β and one γ subunit. These structures shed light on the molecular principles that govern the assembly of distinct ENaC trimers.
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Illuminating cholesterol-mTORC1 signaling: LYCHOS in focus Structure (IF 4.4) Pub Date : 2025-02-06 Hijai R. Shin, Roberto Zoncu
In a recent issue of Nature, Bayly-Jones et al.1 report the first cryoelectron microscopy (cryo-EM) structure of the lysosomal transmembrane protein LYCHOS, which mediates cholesterol sensing by mTORC1. LYCHOS forms a homodimer, with cholesterol engagement at the transporter-GPCR domain interface, coupled to auxin binding at the transporter-like domain, suggesting multi-domain coordination as critical
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Structures of a T1-like siphophage reveal capsid stabilization mechanisms and high structural similarities with a myophage Structure (IF 4.4) Pub Date : 2025-02-05 Can Cai, Yueting Wang, Yunshu Liu, Qianqian Shao, Aohan Wang, Lin Li, Yaqi Zheng, Tianyi Zhang, Ziwen Luo, Chongguang Yang, Qianglin Fang
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The structure of a NEMO construct engineered for screening reveals novel determinants of inhibition Structure (IF 4.4) Pub Date : 2025-02-04 Amy E. Kennedy, Adam H. Barczewski, Christina R. Arnoldy, J. Pepper Pennington, Kelly A. Tiernan, M. Beatriz Hidalgo, Caroline C. Reilly, Tanyawan Wongsri, Michael J. Ragusa, Gevorg Grigoryan, Dale F. Mierke, Maria Pellegrini
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Direct effect of membrane environment on the activation of mGluR2 revealed by single-molecule FRET Structure (IF 4.4) Pub Date : 2025-02-04 Chiranjib Banerjee, Brandon Wey-Hung Liauw, Reza Vafabakhsh
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Architecture of an embracing lipase-foldase complex of the type II secretion system of Acinetobacter baumannii Structure (IF 4.4) Pub Date : 2025-02-03 Yuri Rafael de Oliveira Silva, Carlos Contreras-Martel, Ricardo Rodrigues de Melo, Letícia Maria Zanphorlin, Daniel Maragno Trindade, Andréa Dessen
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Structural basis for substrate binding, catalysis and inhibition of cancer target mitochondrial creatine kinase by a covalent inhibitor Structure (IF 4.4) Pub Date : 2025-02-03 Merve Demir, Laura Koepping, Ya Li, Lynn Fujimoto, Andrey Bobkov, Jianhua Zhao, Taro Hitosugi, Eduard Sergienko
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Water occupancy in the Acinetobacter baumannii F-ATP synthase c-ring and its implications as a novel inhibitor target Structure (IF 4.4) Pub Date : 2025-01-31 Alexander Krah, Vandana Grover, Tuck Choy Fong, Peter J. Bond, Gerhard Grüber
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Septin assemblies promote the lipid organization of membranes Structure (IF 4.4) Pub Date : 2025-01-31 Fatima El Alaoui, Isabelle Al-Akiki, Sandy Ibanes, Sébastien Lyonnais, David Sanchez-Fuentes, Rudy Desgarceaux, Chantal Cazevieille, Marie-Pierre Blanchard, Andrea Parmeggiani, Adrian Carretero-Genevrier, Simonetta Piatti, Laura Picas
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Computational design and improvement of a broad influenza virus HA stem targeting antibody Structure (IF 4.4) Pub Date : 2025-01-29 Huarui Duan, Xiaojing Chi, Xuehua Yang, Shengnan Pan, Xiuying Liu, Peixiang Gao, Fangyuan Zhang, Xinhui Zhang, Xuemeng Dong, Yi Liao, Wei Yang
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Structural insights into polyisoprenyl-binding glycosyltransferases Structure (IF 4.4) Pub Date : 2025-01-29 Allen P. Zinkle, Ryan T. Morgan, Rie Nygaard, Filippo Mancia
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Structure of a zoonotic H5N1 hemagglutinin reveals a receptor-binding site occupied by an auto-glycan Structure (IF 4.4) Pub Date : 2025-01-29 Nicholas C. Morano, Yicheng Guo, Jordan E. Becker, Zhiteng Li, Jian Yu, David D. Ho, Lawrence Shapiro, Peter D. Kwong
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NSUN6 inhibitor discovery guided by its mRNA substrate bound crystal structure Structure (IF 4.4) Pub Date : 2025-01-24 Fumei Zhong, Tian Pu, Qian Hu, Mingwei Li, Lei Wang, Suman Wang, Ke Ruan, Yunyu Shi, Beicheng Sun, Yiyang Jiang, Mengqi Lv
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Structural and functional analysis of SAM-dependent N-methyltransferases involved in ovoselenol and ovothiol biosynthesis Structure (IF 4.4) Pub Date : 2025-01-24 Kendra A. Ireland, Chase M. Kayrouz, Marissa L. Abbott, Mohammad R. Seyedsayamdost, Katherine M. Davis
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Structures and mRNP remodeling mechanism of the TREX-2 complex Structure (IF 4.4) Pub Date : 2025-01-24 Yihu Xie, Bradley P. Clarke, Dongqi Xie, Menghan Mei, Prasanna Bhat, Pate S. Hill, Alexia E. Angelos, Tolga Çağatay, Mariam Haider, Scott E. Collier, Melissa G. Chambers, Vasilisa Aksenova, Mary Dasso, Beatriz M.A. Fontoura, Yi Ren
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Structure-guided insights into TIR-mediated bacterial and eukaryotic immunity Structure (IF 4.4) Pub Date : 2025-01-20 Arpita Chakravarti, Dinshaw J. Patel
Within the course of evolution, TIR (Toll/interleukin-1 receptor) domains acquired a myriad of functional specificities. This has significantly added to their well-established roles in innate immune signaling. These additional functions include nicotinamide adenine dinucleotide (NAD)(P) hydrolase, RNA/DNA nuclease (in plants), CN (cyclic nucleotide) cyclase, and base exchanger activities. Owing to
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Structural variants of AcrIIC5 inhibit Cas9 via divergent binding interfaces Structure (IF 4.4) Pub Date : 2025-01-17 Sung-Hyun Hong, So Young An, Changkon Park, Youngim Kim, Eun-Hee Kim, Nak-Kyoon Kim, Jeong-Yong Suh
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Structural insights into human ABCA7-mediated lipid transport Structure (IF 4.4) Pub Date : 2025-01-17 Shu-Cheng Fang, Liang Wang, Meng-Ting Cheng, Da Xu, Zhi-Peng Chen, Jie Wang, Wenli Liao, Yanyan Li, Cong-Zhao Zhou, Wen-Tao Hou, Yuxing Chen
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Understanding the structural and functional diversity of ATP-PPases using protein domains and functional families in the CATH database Structure (IF 4.4) Pub Date : 2025-01-17 Jialin Yin, Vaishali P. Waman, Neeladri Sen, Mohd Firdaus-Raih, Su Datt Lam, Christine Orengo
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Structural insights into the role of the proline rich region in tau function Structure (IF 4.4) Pub Date : 2025-01-17 Karen Acosta, Christopher R. Brue, Polina Holubovska, Hee Jong Kim, Leland Mayne, Kenji Murakami, Elizabeth Rhoades
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Metal fluorides—multi-functional tools for the study of phosphoryl transfer enzymes, a practical guide Structure (IF 4.4) Pub Date : 2025-01-17 Erika Pellegrini, Pauline Juyoux, Jill von Velsen, Nicola J. Baxter, Hugh R.W. Dannatt, Yi Jin, Matthew J. Cliff, Jonathan P. Waltho, Matthew W. Bowler
(Structure 32, 1834–1846.e1–e3; October 3, 2024)
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Structure of E6AP in complex with HPV16-E6 and p53 reveals a novel ordered domain important for E3 ligase activation Structure (IF 4.4) Pub Date : 2025-01-15 Sebastian Kenny, Shalini Iyer, Clinton A. Gabel, Natalia Tegenfeldt, Andrew G. DeMarco, Mark C. Hall, Leifu Chang, V. Jo Davisson, Scott Vande Pol, Chittaranjan Das
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Mg2+-dependent mechanism of environmental versatility in a multidrug efflux pump Structure (IF 4.4) Pub Date : 2025-01-13 Benjamin Russell Lewis, Muhammad R. Uddin, Katie M. Kuo, Laila M.N. Shah, Nicola J. Harris, Paula J. Booth, Dietmar Hammerschmid, James C. Gumbart, Helen I. Zgurskaya, Eamonn Reading
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The structural biology of deoxyhypusination complexes Structure (IF 4.4) Pub Date : 2025-01-13 Elżbieta Wątor-Wilk, Piotr Wilk, Przemysław Grudnik
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A novel interleukin-10 antibody graft to treat inflammatory bowel disease Structure (IF 4.4) Pub Date : 2025-01-10 Michael DiDonato, Carolina Turk Simpson, Todd Vo, Mark Knuth, Bernhard Geierstanger, Joanna Jamontt, David H. Jones, John W. Fathman, Donnie DeLarosa, Tobias Junt, Damien Picard, Ulrike Sommer, Morten Bagger, Eric Peters, Shelly Meeusen, Glen Spraggon
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Cryo-EM structure and complementary drug efflux activity of the Acinetobacter baumannii multidrug efflux pump AdeG Structure (IF 4.4) Pub Date : 2025-01-10 Zhenlin Ouyang, Wenbo He, Di Wu, Hao An, Lei Duan, Min Jiao, Xiaoyu He, Qinyue Yu, Jiaxin Zhang, Qian Qin, Ruochen Wang, Fang Zheng, Peter M. Hwang, Xiaoting Hua, Li Zhu, Yurong Wen
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Cryo-EM reconstruction of yeast ADP-actin filament at 2.5 Å resolution. A comparison with vertebrate F-actin Structure (IF 4.4) Pub Date : 2025-01-10 Sarah R. Stevenson, Svetomir B. Tzokov, Indrajit Lahiri, Kathryn R. Ayscough, Per A. Bullough
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4D structural biology—The 9th Murnau Conference on structural biology Structure (IF 4.4) Pub Date : 2025-01-02 Janosch Hennig, Cristina Paulino
The data presented at the 9th International Murnau Conference on September 18–21, 2024, the largest recurring structural biology meeting in Central Europe, illustrated the thriving state of the structural biology community. This is largely attributed to the ground-breaking developments over the last decade, which were intensely discussed during the meeting.
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Lysozyme revisited Structure (IF 4.4) Pub Date : 2025-01-02 Esko Oksanen
Lysozyme is a model system for crystallographers. In this issue of Structure, Ramos et al. report atomic resolution neutron structures of lysozyme, which unambiguously show the protonation states and hydrogen-bonding networks of the active site. This resolves mechanistic questions that have been debated for decades and provides a unique view to a protein at atomic detail.
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AI decodes CNNM Na+/Mg2+ exchange Structure (IF 4.4) Pub Date : 2025-01-02 Thushara Nethramangalath, Loren W. Runnels
In this issue of Structure, Ma et al.1 apply the artificial intelligence system AlphaFold2, which was designed to predict three-dimensional protein structures from amino acid sequences with atomic accuracy, to model the conformal dynamics of the prokaryotic TpCorC and human CNNM2 and CNNM4 transporters, providing mechanistic insight into how sodium drives magnesium efflux.
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It takes two to tango: The second membrane-binding site in peripheral proteins Structure (IF 4.4) Pub Date : 2025-01-02 Anand Srivastavava
In this issue of Structure, Soteriou et al.1 use cell biology, in vitro reconstitution approaches, and molecular dynamics (MD) simulations to characterize the membrane association of AKT1. The authors show that the AKT1 pleckstrin homology domain contains two essential and cooperative PI(3,4,5)P3-binding sites that enable stable membrane binding of AKT1 in the requisite orientation required for effective
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Cryoelectron microscopy with elemental sensitivity Structure (IF 4.4) Pub Date : 2025-01-02 Hannah Ochner, Tanmay A.M. Bharat
In a recent issue of Nature Methods, Pfeil-Gardiner et al. (2024)1 combine electron energy-loss spectroscopy and single-particle cryoelectron microscopy to allow the spatially resolved imaging of the elemental composition of macromolecules.
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Structural insights into the biochemical mechanism of the E2/E3 hybrid enzyme UBE2O Structure (IF 4.4) Pub Date : 2024-12-30 Hao Huang, Wenning Zhu, Bin Huang, Ziyang Fu, Yuxian Xiong, Dan Cao, Yuxin Ye, Qing Chang, Wenqi Li, Long Li, Huan Zhou, Xiaogang Niu, Wei Zhang
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The structure and assembly of the hetero-octameric BLOC-one-related complex Structure (IF 4.4) Pub Date : 2024-12-30 Xuan Ge, Jinqi Ren, Kewei Gu, Weibin Gong, Kang Shen, Wei Feng
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Physicochemical features of subunit interfaces and their role in self-assembly across the ferritin superfamily Structure (IF 4.4) Pub Date : 2024-12-30 Amrita, Soumyananda Chakraborti, Sucharita Dey
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The structures of the peptide transporters SLC15A3 and SLC15A4 reveal the recognition mechanisms for substrate and TASL Structure (IF 4.4) Pub Date : 2024-12-23 Zhikuan Zhang, Shota Kasai, Kentaro Sakaniwa, Akiko Fujimura, Umeharu Ohto, Toshiyuki Shimizu
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Antigen presentation by MHC-II is shaped by competitive and cooperative allosteric mechanisms of peptide exchange Structure (IF 4.4) Pub Date : 2024-12-20 Matthias Günther, Jana Sticht, Christian Freund, Thomas Höfer
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Dantrolene inhibition of ryanodine receptor 1 carrying the severe malignant hyperthermia mutation Y522S visualized by cryo-EM Structure (IF 4.4) Pub Date : 2024-12-20 Kavita A. Iyer, Takuya Kobayashi, Takashi Murayama, Montserrat Samsó
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Systematic characterization of indel variants using a yeast-based protein folding sensor Structure (IF 4.4) Pub Date : 2024-12-19 Sven Larsen-Ledet, Søren Lindemose, Aleksandra Panfilova, Sarah Gersing, Caroline H. Suhr, Aitana Victoria Genzor, Heleen Lanters, Sofie V. Nielsen, Kresten Lindorff-Larsen, Jakob R. Winther, Amelie Stein, Rasmus Hartmann-Petersen
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Conformational response of αIIbβ3 and αVβ3 integrins to force Structure (IF 4.4) Pub Date : 2024-12-19 Reza Kolasangiani, Khashayar Farzanian, Yunfeng Chen, Martin A. Schwartz, Tamara C. Bidone
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Near-atomic cryo-EM structure of the light-harvesting complex LH2 from the sulfur purple bacterium Ectothiorhodospira haloalkaliphila Structure (IF 4.4) Pub Date : 2024-12-17 Anna D. Burtseva, Timur N. Baymukhametov, Maxim A. Bolshakov, Zoya К. Makhneva, Andrey V. Mardanov, Andrey M. Tsedilin, Huawei Zhang, Vladimir.O. Popov, Aleksandr A. Ashikhmin, Konstantin M. Boyko
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Structural insights into subunit-dependent functional regulation in epithelial sodium channels Structure (IF 4.4) Pub Date : 2024-12-11 Alexandra Houser, Isabelle Baconguis
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Automated fibril structure calculations in Xplor-NIH Structure (IF 4.4) Pub Date : 2024-12-10 Alexander M. Barclay, Moses H. Milchberg, Owen A. Warmuth, Marcus D. Tuttle, Christopher J. Dennis, Charles D. Schwieters, Chad M. Rienstra
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Ion coupling and inhibitory mechanisms of the human presynaptic high-affinity choline transporter CHT1 Structure (IF 4.4) Pub Date : 2024-12-09 Yunlong Qiu, Yiwei Gao, Qinru Bai, Yan Zhao
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Assembly of the Xrn2/Rat1–Rai1–Rtt103 termination complexes in mesophilic and thermophilic organisms Structure (IF 4.4) Pub Date : 2024-12-09 Alzbeta Dikunova, Nikola Noskova, Jan H. Overbeck, Martin Polak, David Stelzig, David Zapletal, Karel Kubicek, Jiri Novacek, Remco Sprangers, Richard Stefl
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Cost-benefit analysis of cryogenic electron tomography subtomogram averaging of chaperonin MmCpn at near atomic resolution Structure (IF 4.4) Pub Date : 2024-12-06 Yanyan Zhao, Michael F. Schmid, Wah Chiu
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Does the structure of transthyretin amyloid fibrils vary depending on the organ of accumulation? Structure (IF 4.4) Pub Date : 2024-12-05 Mineyuki Mizuguchi
In this issue of Structure, Nguyen et al.1 reveal that amyloid fibrils of the transthyretin (TTR) V30M variant from the heart and nerves of the same patient exhibit structural homogeneity. This finding is crucial for advancing our understanding of V30M-TTR amyloid deposition, which leads to fatal ATTRv amyloidosis.
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A finger that gets in the way: When binding isn’t just about the bound state Structure (IF 4.4) Pub Date : 2024-12-05 David Neuhaus, Katherine Stott
In this issue of Structure, Viennet et al.1 report a study characterizing the DNA binding by a three-zinc-finger fragment from the transcription factor BCL11A, with the unusual feature that an interfinger interaction in the free protein is disrupted during binding, which provides a positive entropic contribution that enhances the affinity.
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The universal Rhs shell structure accommodates various toxins inside and different functional decorations on the outside Structure (IF 4.4) Pub Date : 2024-12-05
In this issue of Structure, Kielkopf et al.1 report the crystal structures of Rhs proteins that are genetically fused to the type VI secretion system …
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Trace_y: Software algorithms for structural analysis of individual helical filaments by three-dimensional contact point reconstruction atomic force microscopy Structure (IF 4.4) Pub Date : 2024-12-05 Wei-Feng Xue
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Mechanistic and structural insights into EstS1 esterase: A potent broad-spectrum phthalate diester degrading enzyme Structure (IF 4.4) Pub Date : 2024-12-05 Shalja Verma, Shweta Choudhary, Kamble Amith Kumar, Jai Krishna Mahto, Anil Kumar Vamsi K, Ishani Mishra, Vellanki Bhanu Prakash, Debabrata Sircar, Shailly Tomar, Ashwani Kumar Sharma, Jitin Singla, Pravindra Kumar
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Structures of TASK-1 and TASK-3 K2P channels provide insight into their gating and dysfunction in disease Structure (IF 4.4) Pub Date : 2024-12-04 Peter Rory Hall, Thibault Jouen-Tachoire, Marcus Schewe, Peter Proks, Thomas Baukrowitz, Elisabeth P. Carpenter, Simon Newstead, Karin E.J. Rödström, Stephen J. Tucker