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Priming enzymes from the pikromycin synthase reveal how assembly-line ketosynthases catalyze carbon-carbon chemistry Structure (IF 5.006) Pub Date : 2022-06-22 Miles S. Dickinson, Takeshi Miyazawa, Ryan S. McCool, Adrian T. Keatinge-Clay
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Inter-subunit crosstalk via PDZ synergistically governs allosteric activation of proapoptotic HtrA2 Structure (IF 5.006) Pub Date : 2022-06-22 Aasna L. Parui, Vandana Mishra, Shubhankar Dutta, Prasenjit Bhaumik, Kakoli Bose
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Structural basis for tunable affinity and specificity of LxCxE-dependent protein interactions with the retinoblastoma protein family Structure (IF 5.006) Pub Date : 2022-06-17 Sivasankar Putta, Lucia Alvarez, Stephan Lüdtke, Peter Sehr, Gerd A. Müller, Samantha M. Fernandez, Sarvind Tripathi, Joe Lewis, Toby J. Gibson, Lucia B. Chemes, Seth M. Rubin
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Binding of the SARS-CoV-2 envelope E protein to human BRD4 is essential for infection Structure (IF 5.006) Pub Date : 2022-06-17 Kendra R. Vann, Arpan Acharya, Suk Min Jang, Catherine Lachance, Mohamad Zandian, Tina A. Holt, Audrey L. Smith, Kabita Pandey, Donald L. Durden, Dalia El-Gamal, Jacques Côté, Siddappa N. Byrareddy, Tatiana G. Kutateladze
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Cullin-independent recognition of HHARI substrates by a dynamic RBR catalytic domain Structure (IF 5.006) Pub Date : 2022-06-17 Katherine H. Reiter, Alex Zelter, Maria K. Janowska, Michael Riffle, Nicholas Shulman, Brendan X. MacLean, Kaipo Tamura, Matthew C. Chambers, Michael J. MacCoss, Trisha N. Davis, Miklos Guttman, Peter S. Brzovic, Rachel E. Klevit
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Structures of an active type III-A CRISPR effector complex Structure (IF 5.006) Pub Date : 2022-06-16 Eric M. Smith, Sé Ferrell, Valerie L. Tokars, Alfonso Mondragón
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Triangulating variation in the population to define mechanisms for precision management of genetic disease Structure (IF 5.006) Pub Date : 2022-06-16 Chao Wang, Frédéric Anglès, William E. Balch
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Disease-linked mutations cause exposure of a protein quality control degron Structure (IF 5.006) Pub Date : 2022-06-13 Caroline Kampmeyer, Sven Larsen-Ledet, Morten Rose Wagnkilde, Mathias Michelsen, Henriette K.M. Iversen, Sofie V. Nielsen, Søren Lindemose, Alberto Caregnato, Tommer Ravid, Amelie Stein, Kaare Teilum, Kresten Lindorff-Larsen, Rasmus Hartmann-Petersen
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The guidance and adhesion protein FLRT2 dimerizes in cis via dual small-X3-small transmembrane motifs Structure (IF 5.006) Pub Date : 2022-06-13 Verity Jackson, Julia Hermann, Christopher J. Tynan, Daniel J. Rolfe, Robin A. Corey, Anna L. Duncan, Maxime Noriega, Amy Chu, Antreas C. Kalli, E. Yvonne Jones, Mark S.P. Sansom, Marisa L. Martin-Fernandez, Elena Seiradake, Matthieu Chavent
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Rational exploration of fold atlas for human solute carrier proteins Structure (IF 5.006) Pub Date : 2022-06-13 Tengyu Xie, Ximin Chi, Bangdong Huang, Fangfei Ye, Qiang Zhou, Jing Huang
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Discovery of isonucleotidic CDNs as potent STING agonists with immunomodulatory potential Structure (IF 5.006) Pub Date : 2022-06-10 Milan Dejmek, Michal Šála, Andrea Brazdova, Lenka Vanekova, Miroslav Smola, Martin Klíma, Petra Břehová, Miloš Buděšínský, Martin Dračínský, Eliška Procházková, Martin Zavřel, Ondřej Šimák, Ondřej Páv, Evzen Boura, Gabriel Birkuš, Radim Nencka
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Uncovering cryptic pockets in the SARS-CoV-2 spike glycoprotein Structure (IF 5.006) Pub Date : 2022-06-03 Lorena Zuzic, Firdaus Samsudin, Aishwary T. Shivgan, Palur V. Raghuvamsi, Jan K. Marzinek, Alister Boags, Conrado Pedebos, Nikhil K. Tulsian, Jim Warwicker, Paul MacAry, Max Crispin, Syma Khalid, Ganesh S. Anand, Peter J. Bond
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Cholesterol occupies the lipid translocation pathway to block phospholipid scrambling by a G protein-coupled receptor Structure (IF 5.006) Pub Date : 2022-06-03 Giulia Morra, Asghar M. Razavi, Anant K. Menon, George Khelashvili
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How insulin-like growth factor I binds to a hybrid insulin receptor type 1 insulin-like growth factor receptor Structure (IF 5.006) Pub Date : 2022-06-03 Yibin Xu, Mai B. Margetts, Hari Venugopal, John G. Menting, Nicholas S. Kirk, Tristan I. Croll, Carlie Delaine, Briony E. Forbes, Michael C. Lawrence
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A comprehensive structural analysis of the ATPase domain of human DNA topoisomerase II beta bound to AMPPNP, ADP, and the bisdioxopiperazine, ICRF193 Structure (IF 5.006) Pub Date : 2022-06-03 Elise M. Ling, Arnaud Baslé, Ian G. Cowell, Bert van den Berg, Tim R. Blower, Caroline A. Austin
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Crystal structure of the lipid flippase MurJ in a “squeezed” form distinct from its inward- and outward-facing forms Structure (IF 5.006) Pub Date : 2022-06-03 Hidetaka Kohga, Takaharu Mori, Yoshiki Tanaka, Kunihito Yoshikaie, Katsuhide Taniguchi, Kei Fujimoto, Lisa Fritz, Tanja Schneider, Tomoya Tsukazaki
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Cytokine aerobics: Oxidation controls cytokine dynamics and function Structure (IF 5.006) Pub Date : 2022-06-02 Markus Brandhofer, Jürgen Bernhagen
In this issue of Structure, Skeens et al. provide insights into the structure and dynamics of an oxidized form of the atypical cytokine macrophage migration-inhibitory factor (MIF). The study unveils a surprising conformational susceptibility of MIF to ambient redox alterations and identifies redox-sensitive residues and latent allostery sites with functional relevance.
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A new twin expands the VirB8-like protein family Structure (IF 5.006) Pub Date : 2022-06-02 Robine Maffo-Woulefack, Nathalie Leblond-Bourget, Badreddine Douzi
Conjugative transfer is mediated by specialized type IV secretion systems (T4SSs). However, their architecture and mode of function remain poorly defined in Gram-positives. In this issue of Structure, Jäger et al. reveal an exclusive assembly of PrgL and illustrate the importance of its structural organization in pCF10 conjugative transfer.
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Dusquetide modulates innate immune response through binding to p62 Structure (IF 5.006) Pub Date : 2022-05-30 Yi Zhang, Christina G. Towers, Upendra K. Singh, Jiuyang Liu, Maria Håkansson, Derek T. Logan, Oreola Donini, Tatiana G. Kutateladze
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Protein language-model embeddings for fast, accurate, and alignment-free protein structure prediction Structure (IF 5.006) Pub Date : 2022-05-23 Konstantin Weissenow, Michael Heinzinger, Burkhard Rost
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Thermodynamic analysis of amyloid fibril structures reveals a common framework for stability in amyloid polymorphs Structure (IF 5.006) Pub Date : 2022-05-23 Rob van der Kant, Nikolaos Louros, Joost Schymkowitz, Frederic Rousseau
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Refolding of lid subdomain of SARS-CoV-2 nsp14 upon nsp10 interaction releases exonuclease activity Structure (IF 5.006) Pub Date : 2022-05-23 Anna Czarna, Jacek Plewka, Leanid Kresik, Alex Matsuda, Abdulkarim Karim, Colin Robinson, Sean O’Byrne, Fraser Cunningham, Irene Georgiou, Piotr Wilk, Magdalena Pachota, Grzegorz Popowicz, Paul Graham Wyatt, Grzegorz Dubin, Krzysztof Pyrć
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Modeling of protein conformational changes with Rosetta guided by limited experimental data Structure (IF 5.006) Pub Date : 2022-05-20 Davide Sala, Diego del Alamo, Hassane S. Mchaourab, Jens Meiler
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Structure-guided optimization of light-activated chimeric G-protein-coupled receptors Structure (IF 5.006) Pub Date : 2022-05-18 Alexandra-Madelaine Tichy, Wang Lok So, Elliot J. Gerrard, Harald Janovjak
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Extracellular domain of PepT1 interacts with TM1 to facilitate substrate transport Structure (IF 5.006) Pub Date : 2022-05-16 Jiemin Shen, Miaohui Hu, Xiao Fan, Zhenning Ren, Corinne Portioli, Xiuwen Yan, Mingqiang Rong, Ming Zhou
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Structural insights into highly similar spatial organization of zinc-finger associated domains with a very low sequence similarity Structure (IF 5.006) Pub Date : 2022-05-16 Artem N. Bonchuk, Konstantin M. Boyko, Alena Y. Nikolaeva, Anna D. Burtseva, Vladimir O. Popov, Pavel G. Georgiev
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A drug and ATP binding site in type 1 ryanodine receptor Structure (IF 5.006) Pub Date : 2022-05-16 Zephan Melville, Haikel Dridi, Qi Yuan, Steven Reiken, Anetta Wronska, Yang Liu, Oliver B. Clarke, Andrew R. Marks
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Structures of oxysterol sensor EBI2/GPR183, a key regulator of the immune response Structure (IF 5.006) Pub Date : 2022-05-09 Hongwen Chen, Weijiao Huang, Xiaochun Li
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The accuracy of protein structures in solution determined by AlphaFold and NMR Structure (IF 5.006) Pub Date : 2022-05-09 Nicholas J. Fowler, Mike P. Williamson
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One enzyme, many substrates: How the vaccinia virus enzyme D9 decaps host and viral mRNAs alike Structure (IF 5.006) Pub Date : 2022-05-05 Vincent D. Maciej, Sutapa Chakrabarti
The decapping enzyme D9 acts on host mRNA and viral double-stranded RNA to promote viral replication in host cells. In this issue of Structure, Peters et al. (2022) present crystal structures of m7GDP-bound D9 to highlight how a conventional fold is adapted to target a wide variety of RNA substrates.
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Cartwheels create symmetry and asymmetry Structure (IF 5.006) Pub Date : 2022-05-05 Takashi Ochi
In this issue of Structure, Kantsadi et al. (2022) present the crystal structures of coiled-coil bundles from SAS-6, a core component of the centriole cartwheel, and reveal that two coiled-coil domains interact asymmetrically. This work provides insights into how the polarity of centrioles is established.
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The GTP responsiveness of PI5P4Kβ evolved from a compromised trade-off between activity and specificity Structure (IF 5.006) Pub Date : 2022-05-02 Koh Takeuchi, Yoshiki Ikeda, Miki Senda, Ayaka Harada, Koji Okuwaki, Kaori Fukuzawa, So Nakagawa, Hong Yang Yu, Lisa Nagase, Misaki Imai, Mika Sasaki, Yu-Hua Lo, Doshun Ito, Natsuki Osaka, Yuki Fujii, Atsuo T. Sasaki, Toshiya Senda
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Structure of an influenza group 2-neutralizing antibody targeting the hemagglutinin stem supersite Structure (IF 5.006) Pub Date : 2022-04-29 Crystal Sao-Fong Cheung, Jason Gorman, Sarah F. Andrews, Reda Rawi, Mateo Reveiz, Chen-Hsiang Shen, Yiran Wang, Darcy R. Harris, Alexandra F. Nazzari, Adam S. Olia, Julie Raab, I-Ting Teng, Raffaello Verardi, Shuishu Wang, Yongping Yang, Gwo-Yu Chuang, Adrian B. McDermott, Tongqing Zhou, Peter D. Kwong
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CryoEM structures of pseudouridine-free ribosome suggest impacts of chemical modifications on ribosome conformations Structure (IF 5.006) Pub Date : 2022-04-29 Yu Zhao, Jay Rai, Hongguo Yu, Hong Li
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Structural insights into cooperative DNA recognition by the CCAAT-binding complex and its bZIP transcription factor HapX Structure (IF 5.006) Pub Date : 2022-04-25 Eva M. Huber, Peter Hortschansky, Mareike T. Scheven, Matthias Misslinger, Hubertus Haas, Axel A. Brakhage, Michael Groll
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Inhibition of Plasmodium falciparum phenylalanine tRNA synthetase provides opportunity for antimalarial drug development Structure (IF 5.006) Pub Date : 2022-04-22 Manmohan Sharma, Nachiappan Mutharasappan, Yogavel Manickam, Karl Harlos, Bruno Melillo, Eamon Comer, Heena Tabassum, Suhel Parvez, Stuart L. Schreiber, Amit Sharma
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Basis for drug selectivity of plasmepsin IX and X inhibition in Plasmodium falciparum and vivax Structure (IF 5.006) Pub Date : 2022-04-22 Anthony N. Hodder, Janni Christensen, Stephen Scally, Tony Triglia, Anna Ngo, Richard W. Birkinshaw, Brodie Bailey, Paola Favuzza, Melanie H. Dietrich, Wai-Hong Tham, Peter E. Czabotar, Kym Lowes, Zhuyan Guo, Nicholas Murgolo, Manuel de Lera Ruiz, John A. McCauley, Brad E. Sleebs, David Olsen, Alan F. Cowman
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Structural and functional insights into the mechanism by which MutS2 recognizes a DNA junction Structure (IF 5.006) Pub Date : 2022-04-18 Kenji Fukui, Masao Inoue, Takeshi Murakawa, Seiki Baba, Takashi Kumasaka, Takato Yano
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Structure of the enterococcal T4SS protein PrgL reveals unique dimerization interface in the VirB8 protein family Structure (IF 5.006) Pub Date : 2022-04-15 Franziska Jäger, Anaïs Lamy, Wei-Sheng Sun, Nina Guerini, Ronnie P-A Berntsson
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Structural basis for llama nanobody recognition and neutralization of HIV-1 at the CD4-binding site Structure (IF 5.006) Pub Date : 2022-04-11 Tongqing Zhou, Lei Chen, Jason Gorman, Shuishu Wang, Young D. Kwon, Bob C. Lin, Mark K. Louder, Reda Rawi, Erik-Stephane D. Stancofski, Yongping Yang, Baoshan Zhang, Anna Forsman Quigley, Laura E. McCoy, Lucy Rutten, Theo Verrips, Robin A. Weiss, Nicole A. Doria-Rose, Peter D. Kwong
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Artificial neural networks for solution scattering data analysis Structure (IF 5.006) Pub Date : 2022-04-11 Dmitry S. Molodenskiy, Dmitri I. Svergun, Alexey G. Kikhney
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Structural characterization of a dimerization interface in the CD28 transmembrane domain Structure (IF 5.006) Pub Date : 2022-04-08 Hongyi Wu, Ruiyu Cao, Maorong Wen, Hongjuan Xue, Bo OuYang
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Mechanistic insight into light-dependent recognition of Timeless by Drosophila Cryptochrome Structure (IF 5.006) Pub Date : 2022-04-08 Changfan Lin, Connor M. Schneps, Siddarth Chandrasekaran, Abir Ganguly, Brian R. Crane
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DEER experiments reveal fundamental differences between calmodulin complexes with IQ and MARCKS peptides in solution Structure (IF 5.006) Pub Date : 2022-04-08 Chandrima Jash, Akiva Feintuch, Shira Nudelman, Nurit Manukovsky, Elwy H. Abdelkader, Sudeshna Bhattacharya, Gunnar Jeschke, Gottfried Otting, Daniella Goldfarb
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Structural characterization of dicyanopyridine containing DNMT1-selective, non-nucleoside inhibitors Structure (IF 5.006) Pub Date : 2022-04-07 John R. Horton, Sarath Pathuri, Kristen Wong, Ren Ren, Lourdes Rueda, David T. Fosbenner, Dirk A. Heerding, Michael T. McCabe, Melissa B. Pappalardi, Xing Zhang, Bryan W. King, Xiaodong Cheng
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An AI-assisted cryo-EM pipeline for structural studies of cellular extracts Structure (IF 5.006) Pub Date : 2022-04-07 Cole Bourque, Björn Udo Klink, Christos Gatsogiannis
Proteins, the building blocks of life, often form large assemblies to perform their function but are traditionally studied separately in structural biology. In this issue of Structure, Skalidis et al. (2022) present a workflow to identify members of intact protein communities and solve their structures de novo to near-atomic resolution.
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Who’s your neighbor? Suggesting alternative assemblies of E/F type bilin lyases from crystal lattice analysis Structure (IF 5.006) Pub Date : 2022-04-07 Maayan Suissa-Szlejf, Jenia Sklyar, Noam Adir
Attachment of bilins to phycobiliproteins is performed by dedicated lyases. In this issue of Structure, Kumarapperuma et al., 2022 present the structure of an E/F type lyase-isomerase that identifies the correct biological interface between active domains, suggesting that a previous E/F lyase misidentified the heterodimer structure from the crystal lattice.
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Who's your neighbor? Suggesting alternative assemblies of E/F type bilin lyases from crystal lattice analysis. Structure (IF 5.006) Pub Date : 2022-04-07 Maayan Suissa-Szlejf,Jenia Sklyar,Noam Adir
Attachment of bilins to phycobiliproteins is performed by dedicated lyases. In this issue of Structure, Kumarapperuma et al., 2022 present the structure of an E/F type lyase-isomerase that identifies the correct biological interface between active domains, suggesting that a previous E/F lyase misidentified the heterodimer structure from the crystal lattice.
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An AI-assisted cryo-EM pipeline for structural studies of cellular extracts. Structure (IF 5.006) Pub Date : 2022-04-07 Cole Bourque,Björn Udo Klink,Christos Gatsogiannis
Proteins, the building blocks of life, often form large assemblies to perform their function but are traditionally studied separately in structural biology. In this issue of Structure, Skalidis et al. (2022) present a workflow to identify members of intact protein communities and solve their structures de novo to near-atomic resolution.
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Structure of SNX9 SH3 in complex with a viral ligand reveals the molecular basis of its unique specificity for alanine-containing class I SH3 motifs Structure (IF 5.006) Pub Date : 2022-04-06 Helena Tossavainen, Hasan Uğurlu, Mikael Karjalainen, Maarit Hellman, Lina Antenucci, Riku Fagerlund, Kalle Saksela, Perttu Permi
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Redox-dependent structure and dynamics of macrophage migration inhibitory factor reveal sites of latent allostery Structure (IF 5.006) Pub Date : 2022-04-04 Erin Skeens, Meagan Gadzuk-Shea, Dilip Shah, Vineet Bhandari, Devin K. Schweppe, Rebecca B. Berlow, George P. Lisi
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Computational studies of the principle of dynamic-change-driven protein interactions Structure (IF 5.006) Pub Date : 2022-04-04 Zhen-lu Li, Carla Mattos, Matthias Buck
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Cross-linking and mass spectrometry as a tool for studying the structural biology of ribonucleoproteins Structure (IF 5.006) Pub Date : 2022-04-01 Chris P. Sarnowski, Maria Bikaki, Alexander Leitner
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Structural characterization of dicyanopyridine containing DNMT1-selective, non-nucleoside inhibitors. Structure (IF 5.006) Pub Date : 2022-04-07 John R Horton,Sarath Pathuri,Kristen Wong,Ren Ren,Lourdes Rueda,David T Fosbenner,Dirk A Heerding,Michael T McCabe,Melissa B Pappalardi,Xing Zhang,Bryan W King,Xiaodong Cheng
DNMT1 maintains the parental DNA methylation pattern on newly replicated hemimethylated DNA. The failure of this maintenance process causes aberrant DNA methylation that affects transcription and contributes to the development and progression of cancers such as acute myeloid leukemia. Here, we structurally characterized a set of newly discovered DNMT1-selective, reversible, non-nucleoside inhibitors
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Deamidation of the human eye lens protein γS-crystallin accelerates oxidative aging Structure (IF 5.006) Pub Date : 2022-03-25 Brenna Norton-Baker, Pedram Mehrabi, Ashley O. Kwok, Kyle W. Roskamp, Megan A. Rocha, Marc A. Sprague-Piercy, David von Stetten, R.J. Dwayne Miller, Rachel W. Martin
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Structure, dynamics, and function of PsDef2 defensin from Pinus sylvestris Structure (IF 5.006) Pub Date : 2022-03-24 Irina Bukhteeva, Natalia I. Hrunyk, Yuri M. Yusypovych, Yulia I. Shalovylo, Valentina Kovaleva, Irina V. Nesmelova
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Structure of the poxvirus decapping enzyme D9 reveals its mechanism of cap recognition and catalysis Structure (IF 5.006) Pub Date : 2022-03-14 Jessica K. Peters, Ryan W. Tibble, Marcin Warminski, Jacek Jemielity, John D. Gross
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A “spindle and thread” mechanism unblocks p53 translation by modulating N-terminal disorder Structure (IF 5.006) Pub Date : 2022-03-14 Margit Kaldmäe, Thibault Vosselman, Xueying Zhong, Dilraj Lama, Gefei Chen, Mihkel Saluri, Nina Kronqvist, Jia Wei Siau, Aik Seng Ng, Farid J. Ghadessy, Pierre Sabatier, Borivoj Vojtesek, Médoune Sarr, Cagla Sahin, Nicklas Österlund, Leopold L. Ilag, Venla A. Väänänen, Saikiran Sedimbi, Michael Landreh
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Quantitative structural analysis of influenza virus by cryo-electron tomography and convolutional neural networks Structure (IF 5.006) Pub Date : 2022-03-14 Qiuyu J. Huang, Kangkang Song, Chen Xu, Daniel N.A. Bolon, Jennifer P. Wang, Robert W. Finberg, Celia A. Schiffer, Mohan Somasundaran
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Crystal structure of the human MUS81-EME2 complex Structure (IF 5.006) Pub Date : 2022-03-14 Zhengkang Hua, Qianqian Fang, Danping Zhang, Zhipu Luo, Cai Yuan, Zhonghui Lin