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Molecular basis of proteolytic cleavage regulation by the extracellular matrix receptor dystroglycan Structure (IF 4.4) Pub Date : 2024-09-20 Michael J.M. Anderson, Amanda N. Hayward, Adam T. Smiley, Ke Shi, Matthew R. Pawlak, Eric J. Aird, Eva Grant, Lauren Greenberg, Hideki Aihara, Robert L. Evans, Christopher Ulens, Wendy R. Gordon
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The human touch: Utilizing AlphaFold 3 to analyze structures of endogenous metabolons Structure (IF 4.4) Pub Date : 2024-09-19 Toni K. Träger, Christian Tüting, Panagiotis L. Kastritis
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Lys716 in the transmembrane domain of yeast mitofusin Fzo1 modulates anchoring and fusion Structure (IF 4.4) Pub Date : 2024-09-18 Raphaëlle Versini, Marc Baaden, Laetitia Cavellini, Mickaël M. Cohen, Antoine Taly, Patrick F.J. Fuchs
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AlphaFold with conformational sampling reveals the structural landscape of homorepeats Structure (IF 4.4) Pub Date : 2024-09-18 David Fernandez Bonet, Shahrayar Ranyai, Luay Aswad, David P. Lane, Marie Arsenian-Henriksson, Michael Landreh, Dilraj Lama
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The future of integrated structural biology Structure (IF 4.4) Pub Date : 2024-09-17 Harald Schwalbe, Pauline Audergon, Natalie Haley, Claudia Alen Amaro, Jon Agirre, Marc Baldus, Lucia Banci, Wolfgang Baumeister, Martin Blackledge, Jose Maria Carazo, Kristina Djinovic Carugo, Patrick Celie, Isabella Felli, Darren J. Hart, Thomas Hauß, Lari Lehtiö, Kresten Lindorff-Larsen, José Márquez, André Matagne, Roberta Pierattelli, Matthias Wilmanns
Instruct-ERIC, “the European Research Infrastructure Consortium for Structural biology research,” is a pan-European distributed research infrastructure making high-end technologies and methods in structural biology available to users. Here, we describe the current state-of-the-art of integrated structural biology and discuss potential future scientific developments as an impulse for the scientific
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Structural characterization of the POTRA domains from A. baumannii reveals new conformations in BamA Structure (IF 4.4) Pub Date : 2024-09-17 Claire Overly Cottom, Robert Stephenson, Dante Ricci, Lixinhao Yang, James C. Gumbart, Nicholas Noinaj
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Rewiring protein binding specificity in paralogous DRG/DFRP complexes Structure (IF 4.4) Pub Date : 2024-09-13 Christian A.E. Westrip, Stephen J. Smerdon, Mathew L. Coleman
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Structural organization of pyruvate: ferredoxin oxidoreductase from the methanogenic archaeon Methanosarcina acetivorans Structure (IF 4.4) Pub Date : 2024-09-11 Matteo Cossu, Daniel Catlin, Sean J. Elliott, William W. Metcalf, Satish K. Nair
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RNA dynamics from experimental and computational approaches Structure (IF 4.4) Pub Date : 2024-09-05 Giovanni Bussi, Massimiliano Bonomi, Paraskevi Gkeka, Michael Sattler, Hashim M. Al-Hashimi, Pascal Auffinger, Maria Duca, Yann Foricher, Danny Incarnato, Alisha N. Jones, Serdal Kirmizialtin, Miroslav Krepl, Modesto Orozco, Giulia Palermo, Samuela Pasquali, Loïc Salmon, Harald Schwalbe, Eric Westhof, Martin Zacharias
Conformational dynamics is crucial for the biological function of RNA molecules and for their potential as therapeutic targets. This meeting report outlines key “take-home” messages that emerged from the presentations and discussions during the CECAM workshop “RNA dynamics from experimental and computational approaches” in Paris, June 26–28, 2023.
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Ordering the disordered Structure (IF 4.4) Pub Date : 2024-09-05 Sarah Shammas, Gabriella Heller, Alaji Bah, Emmanouela Filippidi, Alex Holehouse, Miao Yu, Janin Lautenschläger
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Expanding the insect defensin landscape Structure (IF 4.4) Pub Date : 2024-09-05 Krishnakoli Adhikary, Sébastien F. Poget
In this issue of Structure, Walker et al.1 determined the NMR structure of a recently discovered defensin, Pp19, from the venom of an assassin bug. This peptide adopts an α-defensin-like structure, which had not been observed in insects before. Unlike mammalian α-defensins, which are generally antimicrobial, Pp19 has insecticidal activity.
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Ping, pong, and freeze: Structural insights into the inhibition of ceramide synthase by Fumonisin B1 Structure (IF 4.4) Pub Date : 2024-09-05 Kexin Hu, Yu Cao
Fumonisin B1 (FB1) targets sphingolipid biosynthesis, inhibiting ceramide synthases. In this issue of Structure, Zhang et al.1 determined the cryoelectron microscopic structures of yeast ceramide synthase in complex with FB1 and its acylated derivative, acyl-FB1, revealing a two-step “ping-pong” mechanism for the N-acylation of FB1 and how it inhibits ceramide synthase.
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Antiterminator LoaP loads onto RNA to chase a runaway RNA polymerase Structure (IF 4.4) Pub Date : 2024-09-05 Bing Wang, Irina Artsimovitch
In this issue of Structure, Elghondakly et al.1 present the crystal structure of Thermoanaerobacter pseudethanolicus antiterminator LoaP, a member of a ubiquitous family of NusG transcription factors, bound to its target, a dfn RNA hairpin. LoaP uses RNA as a recognition determinant, which is unique among NusG paralogs and makes unusual contacts in the major groove of the RNA.
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Structural review of SARS-CoV-2 antiviral targets Structure (IF 4.4) Pub Date : 2024-09-05 Wen Cui, Yinkai Duan, Yan Gao, Wei Wang, Haitao Yang
The coronavirus disease 2019 (COVID-19), the disease caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), represents the most disastrous infectious disease pandemic of the past century. As a member of the Betacoronavirus genus, the SARS-CoV-2 genome encodes a total of 29 proteins. The spike protein, RNA-dependent RNA polymerase, and proteases play crucial roles in the virus replication
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Structural insights into the convergent evolution of sulfoxide synthase EgtB-IV, an ergothioneine-biosynthetic homolog of ovothiol synthase OvoA Structure (IF 4.4) Pub Date : 2024-08-30 Kendra A. Ireland, Chase M. Kayrouz, Marissa L. Abbott, Mohammad R. Seyedsayamdost, Katherine M. Davis
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Structure of calcineurin bound to PI4KA reveals dual interface in both PI4KA and FAM126A Structure (IF 4.4) Pub Date : 2024-08-30 Alexandria L. Shaw, Sushant Suresh, Matthew A.H. Parson, Noah J. Harris, Meredith L. Jenkins, Calvin K. Yip, John E. Burke
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Cryo-EM structures of a bathy phytochrome histidine kinase reveal a unique light-dependent activation mechanism Structure (IF 4.4) Pub Date : 2024-08-30 Szabolcs Bódizs, Petra Mészáros, Lukas Grunewald, Heikki Takala, Sebastian Westenhoff
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Novel, tightly structurally related N-myristoyltransferase inhibitors display equally potent yet distinct inhibitory mechanisms Structure (IF 4.4) Pub Date : 2024-08-28 Frédéric Rivière, Cyril Dian, Rémi F. Dutheil, Paul Monassa, Carmela Giglione, Thierry Meinnel
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Unveiling the double-edged sword: SOD1 trimers possess tissue-selective toxicity and bind septin-7 in motor neuron-like cells Structure (IF 4.4) Pub Date : 2024-08-28 Esther Sue Choi, Brianna Hnath, Congzhou Mike Sha, Nikolay V. Dokholyan
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An internal linker and pH biosensing by phosphatidylinositol 5-phosphate regulate the function of the ESCRT-0 component TOM1 Structure (IF 4.4) Pub Date : 2024-08-28 Wen Xiong, Tiffany G. Roach, Nicolas Ball, Marija Corluka, Josephine Beyer, Anne M. Brown, Daniel G.S. Capelluto
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A BTB extension and ion-binding domain contribute to the pentameric structure and TFAP2A binding of KCTD1 Structure (IF 4.4) Pub Date : 2024-08-26 Daniel M. Pinkas, Joshua C. Bufton, Alice E. Hunt, Charlotte E. Manning, William Richardson, Alex N. Bullock
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Cryo-EM structure of the SPFH-NfeD family protein complex QmcA-YbbJ Structure (IF 4.4) Pub Date : 2024-08-23 Kwan Ann Tan, Zhu Qiao, Zachary Ze En Lim, Joshua Yi Yeo, Yonlada Yong, Phong Hoa Do, Ero Rya, Yong-Gui Gao
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Scorpion α-toxin LqhαIT specifically interacts with a glycan at the pore domain of voltage-gated sodium channels Structure (IF 4.4) Pub Date : 2024-08-23 Swastik Phulera, Callum J. Dickson, Christopher J. Schwalen, Maryam Khoshouei, Samantha J. Cassell, Yishan Sun, Tara Condos, Jonathan Whicher, Wilhelm A. Weihofen
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Ca2+-dependent lipid preferences shape synaptotagmin-1 C2A and C2B dynamics: Insights from experiments and simulations Structure (IF 4.4) Pub Date : 2024-08-21 Julian Bender, Til Kundlacz, Lucas S.P. Rudden, Melissa Frick, Julia Bieber, Matteo T. Degiacomi, Carla Schmidt
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Structural basis for FN3K-mediated protein deglycation Structure (IF 4.4) Pub Date : 2024-08-21 Jameela Lokhandwala, Jenet K. Matlack, Tracess B. Smalley, Robert E. Miner, Timothy H. Tran, Jennifer M. Binning
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PB-GPT: An innovative GPT-based model for protein backbone generation Structure (IF 4.4) Pub Date : 2024-08-21 Xiaoping Min, Yiyang Liao, Xiao Chen, Qianli Yang, Junjie Ying, Jiajun Zou, Chongzhou Yang, Jun Zhang, Shengxiang Ge, Ningshao Xia
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Concerted deletions eliminate a neutralizing supersite in SARS-CoV-2 BA.2.87.1 spike Structure (IF 4.4) Pub Date : 2024-08-21 Helen M.E. Duyvesteyn, Aiste Dijokaite-Guraliuc, Chang Liu, Piyada Supasa, Barbara Kronsteiner, Katie Jeffery, Lizzie Stafford, Paul Klenerman, Susanna J. Dunachie, Juthathip Mongkolsapaya, Elizabeth E. Fry, Jingshan Ren, David I. Stuart, Gavin R. Screaton
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Crystal structure of blue laccase BP76, a unique termite suicidal defense weapon Structure (IF 4.4) Pub Date : 2024-08-15 Jana Škerlová, Jiří Brynda, Jan Šobotník, Marek Zákopčaník, Petr Novák, Thomas Bourguignon, David Sillam-Dussès, Pavlína Řezáčová
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Engineering of pH-dependent antigen binding properties for toxin-targeting IgG1 antibodies using light-chain shuffling Structure (IF 4.4) Pub Date : 2024-08-14 Tulika Tulika, Fulgencio Ruso-Julve, Shirin Ahmadi, Anne Ljungars, Esperanza Rivera-de-Torre, Jack Wade, Monica L. Fernández-Quintero, Timothy P. Jenkins, Selma B. Belfakir, Georgina M.S. Ross, Lars Boyens-Thiele, Alexander K. Buell, Siri A. Sakya, Christoffer V. Sørensen, Markus-Frederik Bohn, Line Ledsgaard, Bjørn G. Voldborg, Chiara Francavilla, Tilman Schlothauer, Bruno Lomonte, Andreas H. Laustsen
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Structural requirements for activity of Mind bomb1 in Notch signaling Structure (IF 4.4) Pub Date : 2024-08-08
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Unraveling Rubisco packaging within β-carboxysomes Structure (IF 4.4) Pub Date : 2024-08-08
In this issue of Structure, Kong et al. utilized cryoelectron tomography to closely examine Rubisco packaging within β-carboxysomes. They observed unique Rubisco packaging arrangements that may have important implications for carboxysome structural integrity.
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Descending to inhibit: Antagonist-induced downward shift of VSD II in TPC2 Structure (IF 4.4) Pub Date : 2024-08-08
In this issue of Structure, Chi et al.1 report structural and functional studies that reveal the inhibition mechanism of the lysosomal two-pore channel TPC2 by the antagonist SG-094, which is of interest for drug development. Antagonist binding induces the downward displacement of the voltage-sensor domain II (VSD II), which is accompanied by asymmetric conformational rearrangements of the entire channel
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Insights into the structure of RNPs from segmented negative-sense RNA viruses Structure (IF 4.4) Pub Date : 2024-08-08
The genome of segmented negative-sense single-stranded RNA viruses, such as influenza virus and bunyaviruses, is coated by viral nucleoproteins (NPs), forming a ribonucleoprotein (RNP). In this issue of Structure, Dick et al.1 expand our knowledge on the RNPs of these viruses by solving the structures of Thogoto virus NP and RNP.
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Finding the sweet spot of the insect gustatory receptor Structure (IF 4.4) Pub Date : 2024-08-08
In a recent issue of Nature, Gomes et al.1 utilized structural, experimental, and computational biology to investigate the ligand-gated activation of BmGr9, an insect gustatory receptor specifically tuned to D-fructose. Together with two other studies published elsewhere, they are the first to describe how sugars bind to insect gustatory receptors.
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Structure and assembly of the human IL-12 signaling complex Structure (IF 4.4) Pub Date : 2024-08-06
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Metal fluorides—multi-functional tools for the study of phosphoryl transfer enzymes, a practical guide Structure (IF 4.4) Pub Date : 2024-08-05 Erika Pellegrini, Pauline Juyoux, Jill von Velsen, Nicola J. Baxter, Hugh R.W. Dannatt, Yi Jin, Matthew J. Cliff, Jonathan P. Waltho, Matthew W. Bowler
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Molecular basis of CRX/DNA recognition and stoichiometry at the Ret4 response element Structure (IF 4.4) Pub Date : 2024-07-30
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Automated fiducial-based alignment of cryo-electron tomography tilt series in Dynamo Structure (IF 4.4) Pub Date : 2024-07-29
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Structural basis of phage transcriptional regulation Structure (IF 4.4) Pub Date : 2024-07-26 Chuchu He, Guanchen He, Yu Feng
Phages are the most prevalent and diverse entities in the biosphere and represent the simplest systems that are capable of self-replication. Many fundamental concepts of transcriptional regulation were revealed through phage studies. The replication of phages within bacteria entails the hijacking of the host transcription machinery. Typically, this is accomplished through proteins and RNAs encoded
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Structural basis for the inhibition of βFXIIa by garadacimab Structure (IF 4.4) Pub Date : 2024-07-25
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Molecular mechanism of the endothelin receptor type B interactions with Gs, Gi, and Gq Structure (IF 4.4) Pub Date : 2024-07-22
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Structural insights into the assembly pathway of the Helicobacter pylori CagT4SS outer membrane core complex Structure (IF 4.4) Pub Date : 2024-07-19
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Intracellular tau fragment droplets serve as seeds for tau fibrils Structure (IF 4.4) Pub Date : 2024-07-19
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Structural insights into an atypical histone binding mechanism by a PHD finger Structure (IF 4.4) Pub Date : 2024-07-18 Sabrina Grégoire, Janelle Grégoire, Yidai Yang, Sabrina Capitani, Monika Joshi, Sabina Sarvan, Arvin Zaker, Zhibin Ning, Daniel Figeys, Kathrin Ulrich, Joseph S. Brunzelle, Arvind Mer, Jean-Francois Couture
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Cryo-EM structures reveal the molecular mechanism of HflX-mediated erythromycin resistance in mycobacteria Structure (IF 4.4) Pub Date : 2024-07-18 Krishnamoorthi Srinivasan, Aneek Banerjee, Jayati Sengupta
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Crystal structure of lipase from Pseudomonas aeruginosa reveals an unusual catalytic triad conformation Structure (IF 4.4) Pub Date : 2024-07-17 Gang Xu, Hua Guo, Zhonglang Yu, Shulin Wang, Dandan Shen, Lirong Yang, Jianping Wu, Binbin Chen, Haoran Yu
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They all rock: A systematic comparison of conformational movements in LeuT-fold transporters Structure (IF 4.4) Pub Date : 2024-07-17 Jacob A. Licht, Samuel P. Berry, Michael A. Gutierrez, Rachelle Gaudet
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Berberine analog of chloramphenicol exhibits a distinct mode of action and unveils ribosome plasticity Structure (IF 4.4) Pub Date : 2024-07-16 Zahra Batool, Julia A. Pavlova, Madhura N. Paranjpe, Andrey G. Tereshchenkov, Dmitrii A. Lukianov, Ilya A. Osterman, Alexey A. Bogdanov, Natalia V. Sumbatyan, Yury S. Polikanov
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Structural basis of homodimerization of the JNK scaffold protein JIP2 and its heterodimerization with JIP1 Structure (IF 4.4) Pub Date : 2024-07-15 Laura Mariño Pérez, Francesco S. Ielasi, Alexandra Lee, Elise Delaforge, Pauline Juyoux, Maud Tengo, Roger J. Davis, Andrés Palencia, Malene Ringkjøbing Jensen
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Spike structures, receptor binding, and immune escape of recently circulating SARS-CoV-2 Omicron BA.2.86, JN.1, EG.5, EG.5.1, and HV.1 sub-variants Structure (IF 4.4) Pub Date : 2024-07-15 Linjie Li, Kaiyuan Shi, Yuhang Gu, Zepeng Xu, Chang Shu, Dedong Li, Junqing Sun, Mengqing Cong, Xiaomei Li, Xin Zhao, Guanghui Yu, Songnian Hu, Hui Tan, Jianxun Qi, Xiaopeng Ma, Kefang Liu, George F. Gao
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Structure of the iminium reaction intermediate in an engineered aldolase explains the carboligation activity toward arylated ketones and aldehydes Structure (IF 4.4) Pub Date : 2024-07-15 Hans Hebert, Eda Sönmez, Pasi Purhonen, Mikael Widersten
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Cryo-EM structure of the R388 plasmid conjugative pilus reveals a helical polymer characterized by an unusual pilin/phospholipid binary complex Structure (IF 4.4) Pub Date : 2024-07-12 Abhinav K. Vadakkepat, Songlin Xue, Adam Redzej, Terry K. Smith, Brian T. Ho, Gabriel Waksman
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Pho pictures provide powerful perspectives of phosphate importing proteins Structure (IF 4.4) Pub Date : 2024-07-11 David Speedman, David B. Sauer
In this issue of Structure, Schneider et al.1 report multiple structures of the low-affinity inorganic-phosphate transporter Pho90 from Saccharomyces cerevisiae. With remarkable resolution of the Divalent Anion Sodium Symporter family member, their cryo-EM studies of this fungal protein reveal new modes of sodium, substrate, and lipid binding.
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The merger of two major families of proteins that regulate cellular processes Structure (IF 4.4) Pub Date : 2024-07-11 Karlett J. Parra
In this issue of Structure, Oot and Wilkens1 present new mechanistic insights to finally merge the function of V-ATPase and TLDc domain proteins. They show that TLDc proteins directly affect V-ATPase activity and assembly, expanding our understanding of how V-ATPase and TLDc proteins exert a plethora of biological functions.
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Squishy to crusty: Biophysics reveal the molecular details of FUS droplet maturation Structure (IF 4.4) Pub Date : 2024-07-11 Erich J. Sohn, David S. Libich
In a recent issue of Nature Chemical Biology, Emmanouilidis et al. (2024) investigate the maturation of biomolecular condensates of FUS1-267 and probe the molecular details of droplet aging. They observe that the liquid-to-solid transition of the droplet is mediated at the surface by FUS1–267 molecules that have adopted β-strand conformations.
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Binding of steroid substrates reveals the key to the productive transition of the cytochrome P450 OleP Structure (IF 4.4) Pub Date : 2024-07-05 Antonella Costanzo, Francesca Fata, Ida Freda, Maria Laura De Sciscio, Elena Gugole, Giovanni Bulfaro, Matteo Di Renzo, Luca Barbizzi, Cécile Exertier, Giacomo Parisi, Marco D’Abramo, Beatrice Vallone, Carmelinda Savino, Linda Celeste Montemiglio
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Molecular mechanism of contactin 2 homophilic interaction Structure (IF 4.4) Pub Date : 2024-07-04 Shanghua Fan, Jianfang Liu, Nicolas Chofflet, Aaron O. Bailey, William K. Russell, Ziqi Zhang, Hideto Takahashi, Gang Ren, Gabby Rudenko
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Molecular insights into the initiation step of the Rcs signaling pathway Structure (IF 4.4) Pub Date : 2024-07-03 Nobuhiko Watanabe, Alexei Savchenko