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  • Staphylcoccus aureus metabolites promote IL-10.
    Nat. Microbiol. (IF 15.54) Pub Date : 2020-09-22
    Alice Prince

    Glycolytic Staphylococcus aureus generate lactate that targets specific histone deacetylases to stimulate the production of IL-10 and enable biofilm formation.

    更新日期:2020-09-22
  • Author Correction: A nonlinear time-series analysis approach to identify thresholds in associations between population antibiotic use and rates of resistance.
    Nat. Microbiol. (IF 15.54) Pub Date : 2020-09-21
    José-María López-Lozano,Timothy Lawes,César Nebot,Arielle Beyaert,Xavier Bertrand,Didier Hocquet,Mamoon Aldeyab,Michael Scott,Geraldine Conlon-Bingham,David Farren,Gábor Kardos,Adina Fésűs,Jesús Rodríguez-Baño,Pilar Retamar,Nieves Gonzalo-Jiménez,Ian M Gould,

    An amendment to this paper has been published and can be accessed via a link at the top of the paper.

    更新日期:2020-09-22
  • Discovery of broad-spectrum fungicides that block septin-dependent infection processes of pathogenic fungi.
    Nat. Microbiol. (IF 15.54) Pub Date : 2020-09-21
    Min He,Jia Su,Youpin Xu,Jinhua Chen,Mawsheng Chern,Mingliang Lei,Tuo Qi,Zongkuan Wang,Lauren S Ryder,Bozeng Tang,Míriam Osés-Ruiz,Keke Zhu,Yuyan Cao,Xia Yan,Iris Eisermann,Yuan Luo,Weitao Li,Jing Wang,Junjie Yin,Sin Man Lam,Guoxiong Peng,Xiaofang Sun,Xiaobo Zhu,Bingtian Ma,Jichun Wang,Jiali Liu,Hai Qing,Li Song,Long Wang,Qingqing Hou,Peng Qin,Yan Li,Jing Fan,Deqiang Li,Yuping Wang,Xiue Wang,Ling Jiang

    Many pathogenic fungi depend on the development of specialized infection structures called appressoria to invade their hosts and cause disease. Impairing the function of fungal infection structures therefore provides a potential means by which diseases could be prevented. In spite of this extraordinary potential, however, relatively few anti-penetrant drugs have been developed to control fungal diseases

    更新日期:2020-09-22
  • Spermine-mediated tight sealing of the Magnaporthe oryzae appressorial pore-rice leaf surface interface.
    Nat. Microbiol. (IF 15.54) Pub Date : 2020-09-14
    Raquel O Rocha,Christian Elowsky,Ngoc T T Pham,Richard A Wilson

    Cellular adhesion mediates many important plant–microbe interactions. In the devastating blast fungus Magnaporthe oryzae1, powerful glycoprotein-rich mucilage adhesives2 cement melanized and pressurized dome-shaped infection cells—appressoria—to host rice leaf surfaces. Enormous internal turgor pressure is directed onto a penetration peg emerging from the unmelanized, thin-walled pore at the appressorial

    更新日期:2020-09-14
  • Structures of the stator complex that drives rotation of the bacterial flagellum.
    Nat. Microbiol. (IF 15.54) Pub Date : 2020-09-14
    Justin C Deme,Steven Johnson,Owen Vickery,Amy Muellbauer,Holly Monkhouse,Thomas Griffiths,Rory Hennell James,Ben C Berks,James W Coulton,Phillip J Stansfeld,Susan M Lea

    The bacterial flagellum is the prototypical protein nanomachine and comprises a rotating helical propeller attached to a membrane-embedded motor complex. The motor consists of a central rotor surrounded by stator units that couple ion flow across the cytoplasmic membrane to generate torque. Here, we present the structures of the stator complexes from Clostridium sporogenes, Bacillus subtilis and Vibrio

    更新日期:2020-09-14
  • Antibody-dependent enhancement and SARS-CoV-2 vaccines and therapies.
    Nat. Microbiol. (IF 15.54) Pub Date : 2020-09-09
    Wen Shi Lee,Adam K Wheatley,Stephen J Kent,Brandon J DeKosky

    Antibody-based drugs and vaccines against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are being expedited through preclinical and clinical development. Data from the study of SARS-CoV and other respiratory viruses suggest that anti-SARS-CoV-2 antibodies could exacerbate COVID-19 through antibody-dependent enhancement (ADE). Previous respiratory syncytial virus and dengue virus vaccine

    更新日期:2020-09-10
  • Near-atomic cryo-electron microscopy structures of varicella-zoster virus capsids.
    Nat. Microbiol. (IF 15.54) Pub Date : 2020-09-07
    Wei Wang,Qingbing Zheng,Dequan Pan,Hai Yu,Wenkun Fu,Jian Liu,Maozhou He,Rui Zhu,Yuze Cai,Yang Huang,Zhenghui Zha,Zhenqin Chen,Xiangzhong Ye,Jinle Han,Yuqiong Que,Ting Wu,Jun Zhang,Shaowei Li,Hua Zhu,Z Hong Zhou,Tong Cheng,Ningshao Xia

    Varicella-zoster virus (VZV) is a medically important human herpesvirus that causes chickenpox and shingles, but its cell-associated nature has hindered structure studies. Here we report the cryo-electron microscopy structures of purified VZV A-capsid and C-capsid, as well as of the DNA-containing capsid inside the virion. Atomic models derived from these structures show that, despite enclosing a genome

    更新日期:2020-09-08
  • Anthrax lethal factor cleaves regulatory subunits of phosphoinositide-3 kinase to contribute to toxin lethality.
    Nat. Microbiol. (IF 15.54) Pub Date : 2020-09-07
    Megan A Mendenhall,Shihui Liu,Makayla K Portley,Danielle O'Mard,Rasem Fattah,Roman Szabo,Thomas H Bugge,Jaspal S Khillan,Stephen H Leppla,Mahtab Moayeri

    Anthrax lethal toxin (LT), produced by Bacillus anthracis, comprises a receptor-binding moiety, protective antigen and the lethal factor (LF) protease1,2. Although LF is known to cleave mitogen-activated protein kinase kinases (MEKs/MKKs) and some variants of the NLRP1 inflammasome sensor, targeting of these pathways does not explain the lethality of anthrax toxin1,2. Here we report that the regulatory

    更新日期:2020-09-08
  • Publisher Correction: Keystone taxa indispensable for microbiome recovery.
    Nat. Microbiol. (IF 15.54) Pub Date : 2020-09-02
    Sean M Gibbons

    An amendment to this paper has been published and can be accessed via a link at the top of the paper.

    更新日期:2020-09-02
  • Fluoxazolevir inhibits hepatitis C virus infection in humanized chimeric mice by blocking viral membrane fusion.
    Nat. Microbiol. (IF 15.54) Pub Date : 2020-08-31
    Christopher D Ma,Michio Imamura,Daniel C Talley,Adam Rolt,Xin Xu,Amy Q Wang,Derek Le,Takuro Uchida,Mitsutaka Osawa,Yuji Teraoka,Kelin Li,Xin Hu,Seung Bum Park,Nishanth Chalasani,Parker H Irvin,Andres E Dulcey,Noel Southall,Juan J Marugan,Zongyi Hu,Kazuaki Chayama,Kevin J Frankowski,Tsanyang Jake Liang

    Fluoxazolevir is an aryloxazole-based entry inhibitor of hepatitis C virus (HCV). We show that fluoxazolevir inhibits fusion of HCV with hepatic cells by binding HCV envelope protein 1 to prevent fusion. Nine of ten fluoxazolevir resistance-associated substitutions are in envelope protein 1, and four are in a putative fusion peptide. Pharmacokinetic studies in mice, rats and dogs revealed that fluoxazolevir

    更新日期:2020-08-31
  • Keystone taxa indispensable for microbiome recovery.
    Nat. Microbiol. (IF 15.54) Pub Date : 2020-08-24
    Sean M Gibbons

    Meta-analysis of four human cohort studies reveals specific keystone bacteria associated with gut microbiome recovery after antibiotic exposure.

    更新日期:2020-08-25
  • Early HIV replication revisited.
    Nat. Microbiol. (IF 15.54) Pub Date : 2020-08-24
    Nathalie Jane Arhel

    The dogma that conversion of the RNA genome of HIV into DNA occurs in the cytoplasm before nuclear entry is challenged using nuclear pore blockade experiments.

    更新日期:2020-08-25
  • Transcriptional and functional insights into the host immune response against the emerging fungal pathogen Candida auris.
    Nat. Microbiol. (IF 15.54) Pub Date : 2020-08-24
    Mariolina Bruno,Simone Kersten,Judith M Bain,Martin Jaeger,Diletta Rosati,Michael D Kruppa,Douglas W Lowman,Peter J Rice,Bridget Graves,Zuchao Ma,Yue Ning Jiao,Anuradha Chowdhary,George Renieris,Frank L van de Veerdonk,Bart-Jan Kullberg,Evangelos J Giamarellos-Bourboulis,Alexander Hoischen,Neil A R Gow,Alistair J P Brown,Jacques F Meis,David L Williams,Mihai G Netea

    Candida auris is among the most important emerging fungal pathogens, yet mechanistic insights into its immune recognition and control are lacking. Here, we integrate transcriptional and functional immune-cell profiling to uncover innate defence mechanisms against C. auris. C. auris induces a specific transcriptome in human mononuclear cells, a stronger cytokine response compared with Candida albicans

    更新日期:2020-08-25
  • Large freshwater phages with the potential to augment aerobic methane oxidation.
    Nat. Microbiol. (IF 15.54) Pub Date : 2020-08-24
    Lin-Xing Chen,Raphaël Méheust,Alexander Crits-Christoph,Katherine D McMahon,Tara Colenbrander Nelson,Gregory F Slater,Lesley A Warren,Jillian F Banfield

    There is growing evidence that phages with unusually large genomes are common across various microbiomes, but little is known about their genetic inventories or potential ecosystem impacts. In the present study, we reconstructed large phage genomes from freshwater lakes known to contain bacteria that oxidize methane. Of manually curated genomes, 22 (18 are complete), ranging from 159 kilobase (kb)

    更新日期:2020-08-25
  • Influenza virus repurposes the antiviral protein IFIT2 to promote translation of viral mRNAs.
    Nat. Microbiol. (IF 15.54) Pub Date : 2020-08-24
    Vy Tran,Mitchell P Ledwith,Thiprampai Thamamongood,Christina A Higgins,Shashank Tripathi,Max W Chang,Christopher Benner,Adolfo García-Sastre,Martin Schwemmle,Adrianus C M Boon,Michael S Diamond,Andrew Mehle

    Cells infected by influenza virus mount a large-scale antiviral response and most cells ultimately initiate cell-death pathways in an attempt to suppress viral replication. We performed a CRISPR–Cas9-knockout selection designed to identify host factors required for replication after viral entry. We identified a large class of presumptive antiviral factors that unexpectedly act as important proviral

    更新日期:2020-08-25
  • Diversity and classification of cyclic-oligonucleotide-based anti-phage signalling systems.
    Nat. Microbiol. (IF 15.54) Pub Date : 2020-08-24
    Adi Millman,Sarah Melamed,Gil Amitai,Rotem Sorek

    Cyclic-oligonucleotide-based anti-phage signalling systems (CBASS) are a family of defence systems against bacteriophages (hereafter phages) that share ancestry with the cGAS–STING innate immune pathway in animals. CBASS systems are composed of an oligonucleotide cyclase, which generates signalling cyclic oligonucleotides in response to phage infection, and an effector that is activated by the cyclic

    更新日期:2020-08-25
  • Single-cell RNA-sequencing reports growth-condition-specific global transcriptomes of individual bacteria.
    Nat. Microbiol. (IF 15.54) Pub Date : 2020-08-17
    Fabian Imdahl,Ehsan Vafadarnejad,Christina Homberger,Antoine-Emmanuel Saliba,Jörg Vogel

    Bacteria respond to changes in their environment with specific transcriptional programmes, but even within genetically identical populations these programmes are not homogenously expressed1. Such transcriptional heterogeneity between individual bacteria allows genetically clonal communities to develop a complex array of phenotypes1, examples of which include persisters that resist antibiotic treatment

    更新日期:2020-08-17
  • Mitochondrial dysfunction caused by outer membrane vesicles from Gram-negative bacteria activates intrinsic apoptosis and inflammation.
    Nat. Microbiol. (IF 15.54) Pub Date : 2020-08-17
    Pankaj Deo,Seong H Chow,Mei-Ling Han,Mary Speir,Cheng Huang,Ralf B Schittenhelm,Subhash Dhital,Jack Emery,Jian Li,Benjamin T Kile,James E Vince,Kate E Lawlor,Thomas Naderer

    Sensing of microbes activates the innate immune system, depending on functional mitochondria. However, pathogenic bacteria inhibit mitochondrial activity by delivering toxins via outer membrane vesicles (OMVs). How macrophages respond to pathogenic microbes that target mitochondria remains unclear. Here, we show that macrophages exposed to OMVs from Neisseria gonorrhoeae, uropathogenic Escherichia

    更新日期:2020-08-17
  • Dissimilatory sulfate reduction in the archaeon 'Candidatus Vulcanisaeta moutnovskia' sheds light on the evolution of sulfur metabolism.
    Nat. Microbiol. (IF 15.54) Pub Date : 2020-08-17
    Nikolay A Chernyh,Sinje Neukirchen,Evgenii N Frolov,Filipa L Sousa,Margarita L Miroshnichenko,Alexander Y Merkel,Nikolay V Pimenov,Dimitry Y Sorokin,Sergio Ciordia,María Carmen Mena,Manuel Ferrer,Peter N Golyshin,Alexander V Lebedinsky,Inês A Cardoso Pereira,Elizaveta A Bonch-Osmolovskaya

    Dissimilatory sulfate reduction (DSR)—an important reaction in the biogeochemical sulfur cycle—has been dated to the Palaeoarchaean using geological evidence, but its evolutionary history is poorly understood. Several lineages of bacteria carry out DSR, but in archaea only Archaeoglobus, which acquired DSR genes from bacteria, has been proven to catalyse this reaction. We investigated substantial rates

    更新日期:2020-08-17
  • Comprehensive analysis of IncC plasmid conjugation identifies a crucial role for the transcriptional regulator AcaB.
    Nat. Microbiol. (IF 15.54) Pub Date : 2020-08-17
    Steven J Hancock,Minh-Duy Phan,Zhenyao Luo,Alvin W Lo,Kate M Peters,Nguyen Thi Khanh Nhu,Brian M Forde,Jason Whitfield,Ji Yang,Richard A Strugnell,David L Paterson,Timothy R Walsh,Bostjan Kobe,Scott A Beatson,Mark A Schembri

    The IncC family of broad-host-range plasmids enables the spread of antibiotic resistance genes among human enteric pathogens1,2,3. Although aspects of IncC plasmid conjugation have been well studied4,5,6,7,8,9, many roles of conjugation genes have been assigned based solely on sequence similarity. We applied hypersaturated transposon mutagenesis and transposon-directed insertion-site sequencing to

    更新日期:2020-08-17
  • Author Correction: Genomes of all known members of a Plasmodium subgenus reveal paths to virulent human malaria.
    Nat. Microbiol. (IF 15.54) Pub Date : 2020-08-14
    Thomas D Otto,Aude Gilabert,Thomas Crellen,Ulrike Böhme,Céline Arnathau,Mandy Sanders,Samuel O Oyola,Alain Prince Okouga,Larson Boundenga,Eric Willaume,Barthélémy Ngoubangoye,Nancy Diamella Moukodoum,Christophe Paupy,Patrick Durand,Virginie Rougeron,Benjamin Ollomo,François Renaud,Chris Newbold,Matthew Berriman,Franck Prugnolle

    An amendment to this paper has been published and can be accessed via a link at the top of the paper.

    更新日期:2020-08-15
  • Genetic circuit design automation for yeast.
    Nat. Microbiol. (IF 15.54) Pub Date : 2020-08-03
    Ye Chen,Shuyi Zhang,Eric M Young,Timothy S Jones,Douglas Densmore,Christopher A Voigt

    Cells can be programmed to monitor and react to their environment using genetic circuits. Design automation software maps a desired circuit function to a DNA sequence, a process that requires units of gene regulation (gates) that are simple to connect and behave predictably. This poses a challenge for eukaryotes due to their complex mechanisms of transcription and translation. To this end, we have

    更新日期:2020-08-03
  • Reprogramming of host glutamine metabolism during Chlamydia trachomatis infection and its key role in peptidoglycan synthesis.
    Nat. Microbiol. (IF 15.54) Pub Date : 2020-08-03
    Karthika Rajeeve,Nadine Vollmuth,Sudha Janaki-Raman,Thomas F Wulff,Apoorva Baluapuri,Francesca R Dejure,Claudia Huber,Julian Fink,Maximilian Schmalhofer,Werner Schmitz,Rajeeve Sivadasan,Elmar Wolf,Wolfgang Eisenreich,Almut Schulze,Jürgen Seibel,Thomas Rudel

    Obligate intracellular bacteria such as Chlamydia trachomatis undergo a complex developmental cycle between infectious, non-replicative elementary-body and non-infectious, replicative reticulate-body forms. Elementary bodies transform to reticulate bodies shortly after entering a host cell, a crucial process in infection, initiating chlamydial replication. As Chlamydia fail to replicate outside the

    更新日期:2020-08-03
  • Research funding after COVID-19.
    Nat. Microbiol. (IF 15.54) Pub Date : 2020-08-01
    Miguel Prudêncio,Joana C Costa

    更新日期:2020-08-01
  • Evolutionary origins of the SARS-CoV-2 sarbecovirus lineage responsible for the COVID-19 pandemic.
    Nat. Microbiol. (IF 15.54) Pub Date : 2020-07-28
    Maciej F Boni,Philippe Lemey,Xiaowei Jiang,Tommy Tsan-Yuk Lam,Blair W Perry,Todd A Castoe,Andrew Rambaut,David L Robertson

    There are outstanding evolutionary questions on the recent emergence of human coronavirus SARS-CoV-2 including the role of reservoir species, the role of recombination and its time of divergence from animal viruses. We find that the sarbecoviruses—the viral subgenus containing SARS-CoV and SARS-CoV-2—undergo frequent recombination and exhibit spatially structured genetic diversity on a regional scale

    更新日期:2020-07-28
  • Structures of capsid and capsid-associated tegument complex inside the Epstein-Barr virus.
    Nat. Microbiol. (IF 15.54) Pub Date : 2020-07-27
    Wei Liu,Yanxiang Cui,Caiyan Wang,Zihang Li,Danyang Gong,Xinghong Dai,Guo-Qiang Bi,Ren Sun,Z Hong Zhou

    As the first discovered human cancer virus, Epstein–Barr virus (EBV) causes Burkitt’s lymphoma and nasopharyngeal carcinoma. Isolating virions for determining high-resolution structures has been hindered by latency—a hallmark of EBV infection—and atomic structures are thus available only for recombinantly expressed EBV proteins. In the present study, by symmetry relaxation and subparticle reconstruction

    更新日期:2020-07-27
  • Epigenetic cell fate in Candida albicans is controlled by transcription factor condensates acting at super-enhancer-like elements.
    Nat. Microbiol. (IF 15.54) Pub Date : 2020-07-27
    Corey Frazer,Mae I Staples,Yoori Kim,Matthew Hirakawa,Maureen A Dowell,Nicole V Johnson,Aaron D Hernday,Veronica H Ryan,Nicolas L Fawzi,Ilya J Finkelstein,Richard J Bennett

    Cell identity in eukaryotes is controlled by transcriptional regulatory networks that define cell-type-specific gene expression. In the opportunistic fungal pathogen Candida albicans, transcriptional regulatory networks regulate epigenetic switching between two alternative cell states, ‘white’ and ‘opaque’, that exhibit distinct host interactions. In the present study, we reveal that the transcription

    更新日期:2020-07-27
  • Sulfamethoxazole drug stress upregulates antioxidant immunomodulatory metabolites in Escherichia coli.
    Nat. Microbiol. (IF 15.54) Pub Date : 2020-07-27
    Hyun Bong Park,Zheng Wei,Joonseok Oh,Hao Xu,Chung Sub Kim,Rurun Wang,Thomas P Wyche,Grazia Piizzi,Richard A Flavell,Jason M Crawford

    Escherichia coli is an important model organism in microbiology and a prominent member of the human microbiota1. Environmental isolates readily colonize the gastrointestinal tract of humans and other animals, and they can serve diverse probiotic, commensal and pathogenic roles in the host2,3,4. Although certain strains have been associated with the severity of inflammatory bowel disease (IBD)2,5, the

    更新日期:2020-07-27
  • A marine virus as foe and friend.
    Nat. Microbiol. (IF 15.54) Pub Date : 2020-07-24
    David L Kirchman

    A virus has been found inserted into the genome of the most abundant bacteria in the oceans. Even though the virus can kill its host, the genes carried by the prophage may help these bacteria flourish around the world.

    更新日期:2020-07-24
  • Dual-mechanism antibiotics.
    Nat. Microbiol. (IF 15.54) Pub Date : 2020-07-24
    Ursula Theuretzbacher

    A dual-mechanism compound with antibacterial activity that targets both folate metabolism and membrane integrity, with a favourable resistance profile in vitro, is identified by screening a small molecule library.

    更新日期:2020-07-24
  • Glycan degradation writ large in the ocean.
    Nat. Microbiol. (IF 15.54) Pub Date : 2020-07-24
    Fiona Cuskin,Elisabeth C Lowe

    Marine bacteria that produce an enormous panoply of glycan-degrading enzymes are identified as having a role in releasing fixed carbon present in glycan-rich cell walls of brown algae.

    更新日期:2020-07-24
  • What has the World Health Organization done for us?
    Nat. Microbiol. (IF 15.54) Pub Date : 2020-07-24

    We reflect on why we need the World Health Organization now more than ever.

    更新日期:2020-07-24
  • Rhizobiome shields plants from infection.
    Nat. Microbiol. (IF 15.54) Pub Date : 2020-07-24
    Shaul Pollak,Otto X Cordero

    Iron-scavenging molecules called siderophores that are produced by the native microbial residents of plant roots fend off plant pathogens such as Ralstonia solanacearum.

    更新日期:2020-07-24
  • LY6E impairs coronavirus fusion and confers immune control of viral disease.
    Nat. Microbiol. (IF 15.54) Pub Date : 2020-07-23
    Stephanie Pfaender,Katrina B Mar,Eleftherios Michailidis,Annika Kratzel,Ian N Boys,Philip V'kovski,Wenchun Fan,Jenna N Kelly,Dagny Hirt,Nadine Ebert,Hanspeter Stalder,Hannah Kleine-Weber,Markus Hoffmann,Hans-Heinrich Hoffmann,Mohsan Saeed,Ronald Dijkman,Eike Steinmann,Mary Wight-Carter,Matthew B McDougal,Natasha W Hanners,Stefan Pöhlmann,Tom Gallagher,Daniel Todt,Gert Zimmer,Charles M Rice,John W Schoggins

    Zoonotic coronaviruses (CoVs) are substantial threats to global health, as exemplified by the emergence of two severe acute respiratory syndrome CoVs (SARS-CoV and SARS-CoV-2) and Middle East respiratory syndrome CoV (MERS-CoV) within two decades1,2,3. Host immune responses to CoVs are complex and regulated in part through antiviral interferons. However, interferon-stimulated gene products that inhibit

    更新日期:2020-07-23
  • Doubling of the known set of RNA viruses by metagenomic analysis of an aquatic virome.
    Nat. Microbiol. (IF 15.54) Pub Date : 2020-07-20
    Yuri I Wolf,Sukrit Silas,Yongjie Wang,Shuang Wu,Michael Bocek,Darius Kazlauskas,Mart Krupovic,Andrew Fire,Valerian V Dolja,Eugene V Koonin

    RNA viruses in aquatic environments remain poorly studied. Here, we analysed the RNA virome from approximately 10 l water from Yangshan Deep-Water Harbour near the Yangtze River estuary in China and identified more than 4,500 distinct RNA viruses, doubling the previously known set of viruses. Phylogenomic analysis identified several major lineages, roughly, at the taxonomic ranks of class, order and

    更新日期:2020-07-20
  • Vpu modulates DNA repair to suppress innate sensing and hyper-integration of HIV-1.
    Nat. Microbiol. (IF 15.54) Pub Date : 2020-07-20
    Meta Volcic,Konstantin M J Sparrer,Lennart Koepke,Dominik Hotter,Daniel Sauter,Christina M Stürzel,Myriam Scherer,Thomas Stamminger,Thomas G Hofmann,Nathalie J Arhel,Lisa Wiesmüller,Frank Kirchhoff

    To avoid innate sensing and immune control, human immunodeficiency virus type 1 (HIV-1) has to prevent the accumulation of viral complementary DNA species. Here, we show that the late HIV-1 accessory protein Vpu hijacks DNA repair mechanisms to promote degradation of nuclear viral cDNA in cells that are already productively infected. Vpu achieves this by interacting with RanBP2–RanGAP1*SUMO1–Ubc9 SUMO

    更新日期:2020-07-20
  • Inhibition of the integrated stress response by viral proteins that block p-eIF2-eIF2B association.
    Nat. Microbiol. (IF 15.54) Pub Date : 2020-07-20
    Huib H Rabouw,Linda J Visser,Tim C Passchier,Martijn A Langereis,Fan Liu,Piero Giansanti,Arno L W van Vliet,José G Dekker,Susanne G van der Grein,Jesús G Saucedo,Aditya A Anand,Mikael E Trellet,Alexandre M J J Bonvin,Peter Walter,Albert J R Heck,Raoul J de Groot,Frank J M van Kuppeveld

    Eukaryotic cells, when exposed to environmental or internal stress, activate the integrated stress response (ISR) to restore homeostasis and promote cell survival. Specific stress stimuli prompt dedicated stress kinases to phosphorylate eukaryotic initiation factor 2 (eIF2). Phosphorylated eIF2 (p-eIF2) in turn sequesters the eIF2-specific guanine exchange factor eIF2B to block eIF2 recycling, thereby

    更新日期:2020-07-20
  • A dynamic nomenclature proposal for SARS-CoV-2 lineages to assist genomic epidemiology.
    Nat. Microbiol. (IF 15.54) Pub Date : 2020-07-15
    Andrew Rambaut,Edward C Holmes,Áine O'Toole,Verity Hill,John T McCrone,Christopher Ruis,Louis du Plessis,Oliver G Pybus

    The ongoing pandemic spread of a new human coronavirus, SARS-CoV-2, which is associated with severe pneumonia/disease (COVID-19), has resulted in the generation of tens of thousands of virus genome sequences. The rate of genome generation is unprecedented, yet there is currently no coherent nor accepted scheme for naming the expanding phylogenetic diversity of SARS-CoV-2. Here, we present a rational

    更新日期:2020-07-15
  • Targeting the trypanosome kinetochore with CLK1 protein kinase inhibitors.
    Nat. Microbiol. (IF 15.54) Pub Date : 2020-07-13
    Manuel Saldivia,Eric Fang,Xiaolei Ma,Elmarie Myburgh,Juliana B T Carnielli,Christopher Bower-Lepts,Elaine Brown,Ryan Ritchie,Suresh B Lakshminarayana,Yen-Liang Chen,Debjani Patra,Elizabeth Ornelas,Hazel X Y Koh,Sarah L Williams,Frantisek Supek,Daniel Paape,Richard McCulloch,Marcel Kaiser,Michael P Barrett,Jan Jiricek,Thierry T Diagana,Jeremy C Mottram,Srinivasa P S Rao

    The kinetochore is a macromolecular structure that assembles on the centromeres of chromosomes and provides the major attachment point for spindle microtubules during mitosis. In Trypanosoma brucei, the proteins that make up the kinetochore are highly divergent; the inner kinetochore comprises at least 20 distinct and essential proteins (KKT1–20) that include four protein kinases—CLK1 (also known as

    更新日期:2020-07-13
  • Orally efficacious broad-spectrum allosteric inhibitor of paramyxovirus polymerase.
    Nat. Microbiol. (IF 15.54) Pub Date : 2020-07-13
    Robert M Cox,Julien Sourimant,Mart Toots,Jeong-Joong Yoon,Satoshi Ikegame,Mugunthan Govindarajan,Ruth E Watkinson,Patricia Thibault,Negar Makhsous,Michelle J Lin,Jose R Marengo,Zachary Sticher,Alexander A Kolykhalov,Michael G Natchus,Alexander L Greninger,Benhur Lee,Richard K Plemper

    Paramyxoviruses such as human parainfluenza virus type-3 (HPIV3) and measles virus (MeV) are a substantial health threat. In a high-throughput screen for inhibitors of HPIV3 (a major cause of acute respiratory infection), we identified GHP-88309—a non-nucleoside inhibitor of viral polymerase activity that possesses unusual broad-spectrum activity against diverse paramyxoviruses including respiroviruses

    更新日期:2020-07-13
  • Adenovirus-mediated ubiquitination alters protein-RNA binding and aids viral RNA processing.
    Nat. Microbiol. (IF 15.54) Pub Date : 2020-07-13
    Christin Herrmann,Joseph M Dybas,Jennifer C Liddle,Alexander M Price,Katharina E Hayer,Richard Lauman,Caitlin E Purman,Matthew Charman,Eui Tae Kim,Benjamin A Garcia,Matthew D Weitzman

    Viruses promote infection by hijacking the ubiquitin machinery of the host to counteract or redirect cellular processes. Adenovirus encodes two early proteins, E1B55K and E4orf6, that together co-opt a cellular ubiquitin ligase complex to overcome host defences and promote virus production. Adenovirus mutants lacking E1B55K or E4orf6 display defects in viral RNA processing and protein production, but

    更新日期:2020-07-13
  • Lactate production by Staphylococcus aureus biofilm inhibits HDAC11 to reprogramme the host immune response during persistent infection.
    Nat. Microbiol. (IF 15.54) Pub Date : 2020-07-13
    Cortney E Heim,Megan E Bosch,Kelsey J Yamada,Amy L Aldrich,Sujata S Chaudhari,David Klinkebiel,Casey M Gries,Abdulelah A Alqarzaee,Yixuan Li,Vinai C Thomas,Edward Seto,Adam R Karpf,Tammy Kielian

    Staphylococcus aureus is a leading cause of biofilm-associated prosthetic joint infection (PJI), resulting in considerable disability and prolonged treatment. It is known that host leukocyte IL-10 production is required for S. aureus biofilm persistence in PJI. An S. aureus bursa aurealis Tn library consisting of 1,952 non-essential genes was screened for mutants that failed to induce IL-10 in myeloid-derived

    更新日期:2020-07-13
  • Analytical sensitivity and efficiency comparisons of SARS-CoV-2 RT-qPCR primer-probe sets.
    Nat. Microbiol. (IF 15.54) Pub Date : 2020-07-10
    Chantal B F Vogels,Anderson F Brito,Anne L Wyllie,Joseph R Fauver,Isabel M Ott,Chaney C Kalinich,Mary E Petrone,Arnau Casanovas-Massana,M Catherine Muenker,Adam J Moore,Jonathan Klein,Peiwen Lu,Alice Lu-Culligan,Xiaodong Jiang,Daniel J Kim,Eriko Kudo,Tianyang Mao,Miyu Moriyama,Ji Eun Oh,Annsea Park,Julio Silva,Eric Song,Takehiro Takahashi,Manabu Taura,Maria Tokuyama,Arvind Venkataraman,Orr-El Weizman

    The recent spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) exemplifies the critical need for accurate and rapid diagnostic assays to prompt clinical and public health interventions. Currently, several quantitative reverse transcription–PCR (RT–qPCR) assays are being used by clinical, research and public health laboratories. However, it is currently unclear whether results from

    更新日期:2020-07-10
  • Collective interactions augment influenza A virus replication in a host-dependent manner.
    Nat. Microbiol. (IF 15.54) Pub Date : 2020-07-06
    Kara L Phipps,Ketaki Ganti,Nathan T Jacobs,Chung-Young Lee,Silvia Carnaccini,Maria C White,Miglena Manandhar,Brett E Pickett,Gene S Tan,Lucas M Ferreri,Daniel R Perez,Anice C Lowen

    Infection with a single influenza A virus (IAV) is only rarely sufficient to initiate productive infection. Instead, multiple viral genomes are often required in a given cell. Here, we show that the reliance of IAV on multiple infection can form an important species barrier. Namely, we find that avian H9N2 viruses representative of those circulating widely at the poultry–human interface exhibit acute

    更新日期:2020-07-06
  • Phase-variable capsular polysaccharides and lipoproteins modify bacteriophage susceptibility in Bacteroides thetaiotaomicron.
    Nat. Microbiol. (IF 15.54) Pub Date : 2020-06-29
    Nathan T Porter,Andrew J Hryckowian,Bryan D Merrill,Jaime J Fuentes,Jackson O Gardner,Robert W P Glowacki,Shaleni Singh,Ryan D Crawford,Evan S Snitkin,Justin L Sonnenburg,Eric C Martens

    A variety of cell surface structures dictate interactions between bacteria and their environment, including their viruses (bacteriophages). Members of the human gut Bacteroidetes characteristically produce several phase-variable capsular polysaccharides (CPSs), but their contributions to bacteriophage interactions are unknown. To begin to understand how CPSs have an impact on Bacteroides–phage interactions

    更新日期:2020-06-29
  • Nature Microbiology after #ShutDownSTEM.
    Nat. Microbiol. (IF 15.54) Pub Date : 2020-06-25

    Efforts to improve representation of Black communities in Nature Microbiology start now.

    更新日期:2020-06-26
  • Secrete to beat the heat.
    Nat. Microbiol. (IF 15.54) Pub Date : 2020-06-25
    Kevin R Lauterjung,Neydis Moreno Morales,Megan N McClean

    Cooperative behaviour enables populations of yeast cells to survive high temperatures.

    更新日期:2020-06-26
  • Cholesterol access aids pathogen spread.
    Nat. Microbiol. (IF 15.54) Pub Date : 2020-06-25
    Norma W Andrews

    Some cytosolic bacteria deform the plasma membrane to spread from cell to cell. Secretion of 25-hydroxy-cholesterol by macrophages has emerged as a protective response that depletes the cholesterol pool used by these bacteria to disseminate through epithelia.

    更新日期:2020-06-26
  • Wildlife surveillance for emergent disease.
    Nat. Microbiol. (IF 15.54) Pub Date : 2020-06-25
    Jens H Kuhn

    Monkeypox, a zoonotic disease that is marked in humans by a smallpox-like rash, may present as a rash-less respiratory disease in wild chimpanzees.

    更新日期:2020-06-26
  • Genome-wide associations of human gut microbiome variation and implications for causal inference analyses.
    Nat. Microbiol. (IF 15.54) Pub Date : 2020-06-22
    David A Hughes,Rodrigo Bacigalupe,Jun Wang,Malte C Rühlemann,Raul Y Tito,Gwen Falony,Marie Joossens,Sara Vieira-Silva,Liesbet Henckaerts,Leen Rymenans,Chloë Verspecht,Susan Ring,Andre Franke,Kaitlin H Wade,Nicholas J Timpson,Jeroen Raes

    Recent population-based1,2,3,4 and clinical studies5 have identified a range of factors associated with human gut microbiome variation. Murine quantitative trait loci6, human twin studies7 and microbiome genome-wide association studies1,3,8,9,10,11,12 have provided evidence for genetic contributions to microbiome composition. Despite this, there is still poor overlap in genetic association across human

    更新日期:2020-06-23
  • Author Correction: Structural restrictions for influenza neuraminidase activity promote adaptation and diversification.
    Nat. Microbiol. (IF 15.54) Pub Date : 2020-06-16
    Hao Wang,Dan Dou,Henrik Östbye,Rebecca Revol,Robert Daniels

    An amendment to this paper has been published and can be accessed via a link at the top of the paper.

    更新日期:2020-06-16
  • FOXO1 promotes HIV latency by suppressing ER stress in T cells.
    Nat. Microbiol. (IF 15.54) Pub Date : 2020-06-15
    Albert Vallejo-Gracia,Irene P Chen,Rosalba Perrone,Emilie Besnard,Daniela Boehm,Emilie Battivelli,Tugsan Tezil,Karsten Krey,Kyle A Raymond,Philip A Hull,Marius Walter,Ireneusz Habrylo,Andrew Cruz,Steven Deeks,Satish Pillai,Eric Verdin,Melanie Ott

    Quiescence is a hallmark of CD4+ T cells latently infected with human immunodeficiency virus 1 (HIV-1). While reversing this quiescence is an effective approach to reactivate latent HIV from T cells in culture, it can cause deleterious cytokine dysregulation in patients. As a key regulator of T-cell quiescence, FOXO1 promotes latency and suppresses productive HIV infection. We report that, in resting

    更新日期:2020-06-15
  • Exosome mimicry by a HAVCR1-NPC1 pathway of endosomal fusion mediates hepatitis A virus infection.
    Nat. Microbiol. (IF 15.54) Pub Date : 2020-06-15
    Maria Isabel Costafreda,Abdolrahim Abbasi,Hsinyi Lu,Gerardo Kaplan

    Cell-to-cell communication by exosomes controls normal and pathogenic processes1,2. Viruses can spread in exosomes and thereby avoid immune recognition3. While biogenesis, binding and uptake of exosomes are well characterized4,5, delivery of exosome cargo into the cytoplasm is poorly understood3. We report that the phosphatidylserine receptor HAVCR1 (refs. 6,7) and the cholesterol transporter NPC1

    更新日期:2020-06-15
  • Spatiotemporal proteomics uncovers cathepsin-dependent macrophage cell death during Salmonella infection.
    Nat. Microbiol. (IF 15.54) Pub Date : 2020-06-08
    Joel Selkrig,Nan Li,Annika Hausmann,Matthew S J Mangan,Matylda Zietek,André Mateus,Jacob Bobonis,Anna Sueki,Haruna Imamura,Bachir El Debs,Gianluca Sigismondo,Bogdan I Florea,Herman S Overkleeft,Nataša Kopitar-Jerala,Boris Turk,Pedro Beltrao,Mikhail M Savitski,Eicke Latz,Wolf-Dietrich Hardt,Jeroen Krijgsveld,Athanasios Typas

    The interplay between host and pathogen relies heavily on rapid protein synthesis and accurate protein targeting to ensure pathogen destruction. To gain insight into this dynamic interface, we combined Click chemistry with pulsed stable isotope labelling of amino acids in cell culture to quantify the host proteome response during macrophage infection with the intracellular bacterial pathogen Salmonella

    更新日期:2020-06-08
  • Roadmap for naming uncultivated Archaea and Bacteria.
    Nat. Microbiol. (IF 15.54) Pub Date : 2020-06-08
    Alison E Murray,John Freudenstein,Simonetta Gribaldo,Roland Hatzenpichler,Philip Hugenholtz,Peter Kämpfer,Konstantinos T Konstantinidis,Christopher E Lane,R Thane Papke,Donovan H Parks,Ramon Rossello-Mora,Matthew B Stott,Iain C Sutcliffe,J Cameron Thrash,Stephanus N Venter,William B Whitman,Silvia G Acinas,Rudolf I Amann,Karthik Anantharaman,Jean Armengaud,Brett J Baker,Roman A Barco,Helge B Bode,Eric

    The assembly of single-amplified genomes (SAGs) and metagenome-assembled genomes (MAGs) has led to a surge in genome-based discoveries of members affiliated with Archaea and Bacteria, bringing with it a need to develop guidelines for nomenclature of uncultivated microorganisms. The International Code of Nomenclature of Prokaryotes (ICNP) only recognizes cultures as ‘type material’, thereby preventing

    更新日期:2020-06-08
  • A nutrient-limited screen unmasks rifabutin hyperactivity for extensively drug-resistant Acinetobacter baumannii.
    Nat. Microbiol. (IF 15.54) Pub Date : 2020-06-08
    Brian Luna,Vincent Trebosc,Bosul Lee,Malina Bakowski,Amber Ulhaq,Jun Yan,Peggy Lu,Jiaqi Cheng,Travis Nielsen,Juhyeon Lim,Warisa Ketphan,Hyungjin Eoh,Case McNamara,Nicholas Skandalis,Rosemary She,Christian Kemmer,Sergio Lociuro,Glenn E Dale,Brad Spellberg

    Industry screens of large chemical libraries have traditionally relied on rich media to ensure rapid bacterial growth in high-throughput testing. We used eukaryotic, nutrient-limited growth media in a compound screen that unmasked a previously unknown hyperactivity of the old antibiotic, rifabutin (RBT), against highly resistant Acinetobacter baumannii. In nutrient-limited, but not rich, media, RBT

    更新日期:2020-06-08
  • Nuclear pore blockade reveals that HIV-1 completes reverse transcription and uncoating in the nucleus.
    Nat. Microbiol. (IF 15.54) Pub Date : 2020-06-01
    Adarsh Dharan,Niklas Bachmann,Sarah Talley,Virginia Zwikelmaier,Edward M Campbell

    Retroviral infection involves the reverse transcription of the viral RNA genome into DNA, which is subsequently integrated into the host cell genome. Human immunodeficiency virus type 1 (HIV-1) and other lentiviruses mediate the infection of non-dividing cells through the ability of the capsid protein1 to engage the cellular nuclear import pathways of the target cell and mediate their nuclear translocation

    更新日期:2020-06-01
  • Structural insights into assembly, operation and inhibition of a type I restriction-modification system.
    Nat. Microbiol. (IF 15.54) Pub Date : 2020-06-01
    Yina Gao,Duanfang Cao,Jingpeng Zhu,Han Feng,Xiu Luo,Songqing Liu,Xiao-Xue Yan,Xinzheng Zhang,Pu Gao

    Type I restriction–modification (R–M) systems are widespread in prokaryotic genomes and provide robust protection against foreign DNA. They are multisubunit enzymes with methyltransferase, endonuclease and translocase activities. Despite extensive studies over the past five decades, little is known about the molecular mechanisms of these sophisticated machines. Here, we report the cryo-electron microscopy

    更新日期:2020-06-01
  • Heads or tails for type V pilus assembly.
    Nat. Microbiol. (IF 15.54) Pub Date : 2020-05-28
    Lori L Burrows

    Protease-enabled strand exchange to mediate assembly of type V pilus is confirmed using a combination of cryogenic electron microscopy and X-ray crystal structures.

    更新日期:2020-05-28
  • Streptomyces' scent attracts spore dispersers.
    Nat. Microbiol. (IF 15.54) Pub Date : 2020-05-28
    Marko Rohlfs

    Earthy-smelling volatiles produced by sporulating Streptomyces attract soil arthropods that eat the bacteria and spread ‘hitch-hiking’ spores.

    更新日期:2020-05-28
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