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Derivation of an Upscaled Model for Xylitol Production With Immobilized Microorganisms Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-02-17 Rolando Zenteno-Catemaxca, Roel Hernandez-Rodriguez, Epifanio Morales-Zárate, Eliseo Hernandez-Martinez
Xylitol, a polyalcohol with anticariogenic properties, finds broad applications in different sectors. Research into new production methods has highlighted the biochemical route using immobilized microorganisms. However, challenges remain in optimizing operating conditions and understanding mass transport and biochemical reactions. Different macroscopic models have been proposed to address these challenges
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Development of Methods to Produce SARS CoV‐2 Virus‐Like Particles at Scale Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-02-12 Melissa A. Edeling, Linda Earnest, Julio Carrera Montoya, Ashley Huey Yiing Yap, Jamie Mumford, Jason Roberts, Chinn Yi Wong, Dhiraj Hans, Joseph Grima, Nicole Bisset, Jesse Bodle, Steven Rockman, Joseph Torresi
The devastating global toll precipitated by the SARS CoV‐2 outbreak and the profound impact of vaccines in stemming that outbreak has established the need for molecular platforms capable of rapidly delivering effective, safe and accessible medical interventions in pandemic preparedness. We describe a simple, efficient and adaptable process to produce SARS CoV‐2 virus‐like particles (VLPs) that can
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Zeolitic Imidazole Framework‐8 Nanoparticles as an Alternative to Freund's Adjuvant for Klebsiella pneumoniae Recombinant Protein Vaccine Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-02-12 Gaowei Hu, Chunli Hong, Yingjie Miao, Wenji Wang, Longfei Yin, Xi Luo, Yongqian Fu
Vaccination represents a promising approach to combat resistant Klebsiella pneumoniae (KP). However, there is currently no licensed vaccine in the veterinary field. Outer membrane proteins have been proven to possess good immunogenicity, but Freund's adjuvant, which is commonly used to administer protein vaccines, has limitations such as a complicated formulation process as well as a tendency to cause
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Enhanced Natural Killer Cell Proliferation by Stress‐Induced Feeder Cells Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-02-11 Donghyun Lee, Myeongkwan Song, Soonjo Kwon
Natural killer (NK) cells, integral to the innate immune system, are notable in cell therapies because of their applicability in allogeneic treatments, distinguishing them from T cells typically employed in conventional cell therapies. However, their limited half‐life (proliferative capability) poses a challenge for therapy. The limited half‐life creates difficulties in obtaining a sufficient number
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Assessing the Adjuvant Potential of Chinese Hamster Ovary Host Cell Proteins Using an In Vitro Dendritic Cell Assay Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-02-11 Sherin Panikulam, Hannah Morgan, Michael Gutknecht, Anette Karle, Atchaya Rajaratnam, Jennifer Muntwyler, Oliver Anderka, Nicolas Lebesgue, Thomas K. Villiger
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Biotechnology and Bioengineering: Volume 122, Number 3, March 2025 Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-02-11
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Enhancing Biomass Productivity by Forecast‐Informed Pond Operations Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-02-07 Hongxiang Yan, Mark S. Wigmosta, Ning Sun, Song Gao, Michael H. Huesemann
Microalgal cultivation for biofuels and proteins holds significant promise but faces challenges in achieving economically viable biomass productivity under variable environmental conditions. This study introduces a forecast‐informed pond operation (FIPO) system that uses numerical weather prediction (NWP) ensemble forecasts and the biomass assessment tool (BAT) to optimize daily dilution rates for
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One‐Pot Hetero‐Di‐C‐Glycosylation of the Natural Polyphenol Phloretin by a Single C‐Glycosyltransferase With Broad Sugar Substrate Specificity Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-02-07 Tuo Li, Annika J. E. Borg, Leo Krammer, Rolf Breinbauer, Bernd Nidetzky
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Systematic Development of a Detergent Toolbox as an Alternative to Triton X‐100 Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-02-06 Varsha Yadav, Mary Lunson, Hannah Shore, Jean Aucamp
Detergents are routinely included in protein purification processes to inactivate enveloped viruses that may arise from adventitious or endogenous contamination. The detergent Triton X‐100 (TX‐100) has been widely used as part of the production process for therapeutic proteins. However, recent ecological studies indicate that TX‐100 and its metabolites detrimentally impact aquatic organisms, thus alternative
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Construction of a CRISPR‐Cas9‐Based Genetic Editing Tool for Serratia marcescens Using a Stationary Phase Promoter and Its Application in Putrescine Production Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-02-06 Linbo Gou, Di Liu, Tai‐Ping Fan, Huaxiang Deng, Yujie Cai
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Demonstrating the Effectiveness of an Alternative to Triton X‐100 for Detergent‐Mediated Viral Inactivation in Biomanufacturing Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-02-05 Kakolie Banerjee, Alice Antonello, Sandra Johnson, Anja Licht, Almut Rapp, Corinne Miller
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Chromatography in Downstream Processing of Recombinant Adeno‐Associated Viruses: A Review of Current and Future Practises Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-02-05 Julius Klemens Lorek, Madelène Isaksson, Bernt Nilsson
Recombinant adeno‐associated virus (rAAV) has emerged as an attractive gene delivery vector platform to treat both rare and pervasive diseases. With more and more rAAV‐based therapies entering late‐stage clinical trials and commercialization, there is an increasing pressure on the rAAV manufacturing process to accelerate drug development, account for larger trials, and commercially provide high doses
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Machine Learning‐Powered Optimization of a CHO Cell Cultivation Process Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-01-31 Jannik Richter, Qimin Wang, Ferdinand Lange, Phil Thiel, Nina Yilmaz, Dörte Solle, Xiaoying Zhuang, Sascha Beutel
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Effects of Air Temperature on Transient Expression of Influenza Hemagglutinin in Nicotiana benthamiana: Analysis of Transgene Transcription and Plant Stress Responses Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-01-31 Patthasarun Pruksarojanakul, Kazune Atsumi, Youngjun Oh, Nobuyuki Matoba, Ryo Matsuda
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pH Threshold Impacts Chalcopyrite Bioleaching Dynamics for the Extreme Thermoacidophile Sulfurisphaera ohwakuensis Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-01-31 Daniel J. Willard, Mohammad J. H. Manesh, Kaitlyn M. John, Robert M. Kelly
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A New Modeling Approach for Predicting the Growth of Filamentous Algae in Outdoor Algae‐Based Wastewater Treatment Systems Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-01-31 Sulochana Pitawala, Peter J. Scales, Gregory J. O. Martin
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Alterations in Protein Phosphorylation and Arginine Biosynthesis Metabolism Confer β‐Phenylethanol Tolerance in Saccharomyces cerevisiae Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-01-31 Chenghan Yang, Yilin Ren, Li Zhang, Yina Li, Chunxia Wang, Haifeng Hang, Xiwei Tian, Ali Mohsin, Ju Chu, Yingping Zhuang
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Modeling the Performance of an Anaerobic Moving Bed Biofilm Reactor Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-01-31 Yuhang Cai, Joshua P. Boltz, Bruce E. Rittmann
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Effectively Guiding Cell Elongation and Alignment by Constructing Micro/Nano Hierarchical Patterned Titania on Titanium Substrate Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-01-30 Feng‐Jiao Bai, Hui Wang, Yu‐Qing Hu, Yun‐Fei Shao, Yi‐Ran Zhu, Yu‐Lin Jiang, Jian‐Chen Hu, Hui‐Jing Zhao, Ke‐Qin Zhang
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Proteo‐Transcriptomic Analysis Reveals the Mechanisms Underlying Escherichia coli Phenotypic Shifts Under Blue Light Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-01-29 Shuqi Shi, Wenlu Qi, Jinming Zhang, Caice Liang, Wei Liu, Hui Han, Wei Zhuang, Tianpeng Chen, Wenjun Sun, Yong Chen
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High‐Efficiency CRISPR‐Cas9 Genome Editing Unveils Biofilm Insights and Enhances Antimicrobial Activity in Bacillus velezensis FZB42 Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-01-28 Na Guo, Shangjun Wang, Christopher Tyler Whitfield, William D. Batchelor, Yifen Wang, David Blersch, Brendan T. Higgins, Yucheng Feng, Mark R. Liles, Luz E. de‐Bashan, Yi Wang, Yuechao Ma
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Graphene Oxide‐Assisted Tapered Microfiber Super‐Sensor for Rapid Detection of Mycobacterium tuberculosis Antigens Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-01-27 Ren Liu, Tianhuan Song, Xiaolin Chen, Zhiheng Yu, Cunliang Yang, Xianchao Zhang, Fengli Huang, Hao Jia, Jijun Feng, Yujiong Wang
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A Dive Into Yeast's Sugar Diet—Comparing the Metabolic Response of Glucose, Fructose, Sucrose, and Maltose Under Dynamic Feast/Famine Conditions Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-01-27 Koen Johannes Anthonius Verhagen, Ilse Henrike Pardijs, Hendrik Matthijs van Klaveren, Sebastian Aljoscha Wahl
Microbes experience dynamic conditions in natural habitats as well as in engineered environments, such as large‐scale bioreactors, which exhibit increased mixing times and inhomogeneities. While single perturbations have been studied for several organisms and substrates, the impact of recurring short‐term perturbations remains largely unknown. In this study, we investigated the response of Saccharomyces
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Application of Multiple Base‐Editing Mediated by Polycistronic tRNA‐gRNA‐Processing System in Pig Cells Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-01-23 Wudi Zhao, Xiangxing Zhu, Guobin Huang, Hao Gu, Yanzhen Bi, Dongsheng Tang, Hongyan Ren
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Reducing Structural Nonidentifiabilities in Upstream Bioprocess Models Using Profile‐Likelihood Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-01-18 Heiko Babel, Ola Omar, Albert Paul, Joachim Bär
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Real‐Time Auto Controlling of Viable Cell Density in Perfusion Cultivation Aided by In‐Line Dielectric Spectroscopy With Segmented Adaptive PLS Model Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-01-18 Yunpeng Sun, Qiongqiong Zhang, Yunfei He, Dongliang Chen, Zheyu Wang, Xiang Zheng, Mingyue Fang, Hang Zhou
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A Novel Screening System to Characterize and Engineer Quorum Quenching Lactonases Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-01-18 Kitty Sompiyachoke, Joseph Bravo, Rakesh Sikdar, Jowan Abdullah, Mikael H. Elias
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Continuous Production of Influenza VLPs Using IC‐BEVS and Multi‐Stage Bioreactors Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-01-18 Ricardo Correia, Taja Zotler, Francisco Ferraz, Bárbara Fernandes, Miguel Graça, Gorben P. Pijlman, Paula M. Alves, António Roldão
The insect cell‐baculovirus expression vector system (IC‐BEVS) has been an asset to produce biologics for over 30 years. With the current trend in biotechnology shifting toward process intensification and integration, developing intensified processes such as continuous production is crucial to hold this platform as a suitable alternative to others. However, the implementation of continuous production
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Creating a Halotolerant Degrader for Efficient Mineralization of p‐Nitrophenol‐Substituted Organophosphorus Pesticides in High‐Saline Wastewater Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-01-17 Yujie Liu, Weini Xiong, Yuting Jiang, Yan Meng, Wanwan Zhao, Chao Yang, Ruihua Liu
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Dynamic Culture of Bioprinted Liver Tumor Spheroids in a Pillar/Perfusion Plate for Predictive Screening of Anticancer Drugs Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-01-17 Pranav Joshi, Hamilton Silva do Nascimento, Soo‐Yeon Kang, Minseong Lee, Manav Goud Vanga, Sang‐Hyun Lee, Bosung Ku, Moyses dos Santos Miranda, Moo‐Yeal Lee
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A Paradigm of Computer Vision and Deep Learning Empowers the Strain Screening and Bioprocess Detection Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-01-17 Feng Xu, Lihuan Su, Yuan Wang, Kaihao Hu, Ling Liu, Rong Ben, Hao Gao, Ali Mohsin, Ju Chu, Xiwei Tian
High‐performance strain and corresponding fermentation process are essential for achieving efficient biomanufacturing. However, conventional offline detection methods for products are cumbersome and less stable, hindering the “Test” module in the operation of “Design‐Build‐Test‐Learn” cycle for strain screening and fermentation process optimization. This study proposed and validated an innovative research
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Manganese Peroxidase Participates in the Liquid–Solid–Gas Triphase Regulation on Microbial Degradation of Lignocellulose in Solid‐State Fermentation Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-01-15 Lei Zhu, Juan Ding, Wenjing Xue, Shu Zhou, Longyu Wang, Ailiang Jiang, Mingwen Zhao, Qin He, Ang Ren
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Engineering Gene and Protein Switches for Regulation of Lineage‐Specifying Transcription Factors Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-01-13 Ana P. Teixeira, Nik Franko, Martin Fussenegger
Human pluripotent stem cells (hPSCs) can be differentiated in vitro to an increasing number of mature cell types, presenting significant promise for addressing a wide range of diseases and studying human development. One approach to further enhance stem cell differentiation methods would be to coordinate multiple inducible gene or protein switches to operate simultaneously within the same cell, with
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Heterologous Expression and Optimization of Fermentation Conditions for Recombinant Ikarugamycin Production Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-01-12 Julia K. Evers, Anna Glöckle, Monique Wiegand, Sebastian Schuler, Manuel Einsiedler, Tobias A. M. Gulder
Ikarugamycin is a member of the natural product family of the polycyclic tetramate macrolactams (PoTeMs). The compound exhibits a diverse range of biological activities, including antimicrobial, antiprotozoal, anti‐leukemic, and anti‐inflammatory properties. In addition, it interferes with several crucial cellular functions, such as oxidized low‐density lipoprotein uptake in macrophages, Nef‐induced
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Biotechnology and Bioengineering: Volume 122, Number 2, February 2025 Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-01-10
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The Effect of Accessibility of Insoluble Substrate on the Overall Kinetics of Enzymatic Degradation Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-01-07 Zdeněk Petrášek, Bernd Nidetzky
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Genome‐Scale Community Model‐Guided Development of Bacterial Coculture for Lignocellulose Bioconversion Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-01-06 Pritam Kundu, Amit Ghosh
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A Novel, Site‐Specific N‐Linked Glycosylation Model Provides Mechanistic Insights Into the Process‐Condition Dependent Distinct Fab and Fc Glycosylation of an IgG1 Monoclonal Antibody Produced by CHO VRC01 Cells Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-12-31 Jayanth Venkatarama Reddy, Thomas Leibiger, Sumit Kumar Singh, Kelvin H. Lee, Eleftherios Papoutsakis, Marianthi Ierapetritou
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Predictions to Increase Lasso Peptide Production in the Heterologous Host Streptomyces coelicolor M1152 Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-12-30 Valeria Razmilic, Juan A. Asenjo, Irene Martínez
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H2 Consumption by Various Acetogenic Bacteria Follows First‐Order Kinetics up to H2 Saturation Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-12-28 Laura Muñoz‐Duarte, Susmit Chakraborty, Louise Vinther Grøn, Maria Florencia Bambace, Jacopo Catalano, Jo Philips
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CFD‐Based Determination of Optimal Design and Operating Conditions of a Fermentation Reactor Using Bayesian Optimization Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-12-28 Hongbum Choi, Kosan Roh, Jay H. Lee
The efficiency of fermentation reactors is significantly impacted by gas dispersion and concentration distribution, which are influenced by the reactor's design and operating conditions. As the process scales up, optimizing these parameters becomes crucial due to the pronounced concentration gradients that can arise. This study integrates the kinetics of the fermentation process with hydrodynamic analysis
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Dual‐Stage Cross‐Flow Filtration: Integrated Capture and Purification of Virus‐Like Particles Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-12-26 Annabelle Dietrich, Luca Heim, Jürgen Hubbuch
Virus‐like particles (VLPs) are a versatile technology for the targeted delivery of genetic material through packaging and potential surface modifications for directed delivery or immunological issues. Although VLP production is relatively simple as they can be recombinantly produced using microorganisms such as Escherichia coli, their current downstream processing often relies on individually developed
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Recent Research on Chondrocyte Dedifferentiation and Insights for Regenerative Medicine Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-12-24 Weixian Su, Yupeng Nie, Shicong Zheng, Yongchang Yao
Chondrocytes maintain the balance of the extracellular matrix by synthesizing glycoproteins, collagen, proteoglycans and hyaluronic acid. Chondrocyte dedifferentiation refers to a process in which chondrocytes lose their mature differentiated phenotype and transform into a fibroblast‐like morphology with fewer differentiated stages and inferior function under external stimulation. The important mechanism
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Open‐Source Anaerobic Digestion Modeling Platform, Anaerobic Digestion Model No. 1 Fast (ADM1F) Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-12-24 Kuang Zhu, Wenjuan Zhang, Elchin Jafarov, Satish Karra, Kurt Solander, Meltem Urgun Demirtas, Lutgarde Raskin, Steven Skerlos
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Impact of Hormone on Growth and GA3 Regulation of Anthocyanin Biosynthesis in Suspension‐Culture Cells of Cyclocarya paliurus Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-12-23 Chuanqing Pan, Yuan Liu, Daobang Tang, Jiguang Chen, Zhongping Yin
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Research on Intelligent Monitoring and Concentration Prediction for Penicillin Fermentation Process Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-12-22 Yin Zhang, Kai Zhang, Ting Hu, Libo Yuan
In the biopharmaceutical industry, accurately predicting penicillin concentration during fermentation is key to boosting production efficiency and quality assurance. This study leverages the PenSim simulation data set and applies various machine learning and deep learning techniques to forecast penicillin fermentation concentration. Initially, through correlation analysis, nine feature variables with
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Efficient Multiplex Genome Editing of the Cyanobacterium Synechocystis sp. PCC6803 via CRISPR-Cas12a Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-12-19 Wei Du, Luna L. Meister, Tobias van Grinsven, Filipe Branco dos Santos
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Functional Expression and Construction of a Self-Sufficient Cytochrome P450 Chimera for Efficient Steroidal C14α Hydroxylation in Escherichia coli Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-12-19 Xia Ke, Hong-Duo Dong, Xi-Man Zhao, Xin-Xin Wang, Zhi-Qiang Liu, Yu-Guo Zheng
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In Situ, High-Resolution Quantification of CO2 Uptake Rates via Automated Off-Gas Analysis Illuminates Carbon Uptake Dynamics in Cyanobacterial Cultures Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-12-18 Christopher M. Jones, Sean Innes, Steven Holland, Tyson Burch, Sydney Parrish, David R. Nielsen
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Biosynthesis of Bacteriochlorophylls and Bacteriochlorophyllides in Escherichia coli Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-12-17 Baiyang Wang, Qiancheng Liao, Chenyang Xia, Fei Gan
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Mesenchymal Stem Cells-Derived Small Extracellular Vesicles and Their Validation as a Promising Treatment for Chondrosarcoma in a 3D Model in Vitro Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-12-17 Eugenia Romano, Francesca Perut, Sofia Avnet, Gemma Di Pompo, Simona Silvestri, Felicia Roffo, Nicola Baldini, Paolo Antonio Netti, Enza Torino
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Kinetic Modeling of the Antibody Disulfide Bond Reduction Reaction With Integrated Prediction of the Drug Load Profile for Cysteine-Conjugated ADCs Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-12-17 Jan Tobias Weggen, Pedro González, Kimberly Hui, Ryan Bean, Michaela Wendeler, Jürgen Hubbuch
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An Additive-Fabricated Biphasic Scaffold for Procedurally Promoting Bone Regeneration via Antioxidant and Osteogenesis Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-12-16 Chunyu Han, Zhenxu Wu, Yuqi Gao, Shuang Yang, Yu Wang, Min Guo, Yueyue Li, Wanzhong Yin, Ling Liu, Wenzhi Song, Peibiao Zhang, Liqiang Wang
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3D Printed GelMA/CHIMA Cross-Linked Network Hydrogel for Angiogenesis Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-12-11 Zixian Liu, Meng Li, Rong Cheng, Lijing Wang, Peiyi Hou, Lu Han, Shengbo Sang
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Biotechnology and Bioengineering: Volume 122, Number 1, January 2025 Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-12-11
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Fluorescent Reporter-Based Fiber Optic Probe for Continuous Detection of Antibodies Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-12-10 Suman Nandy, Binh V. Vu, Vijay M. Maranholkar, Atul Goyal, Katerina Kourentzi, Richard C. Willson
Measurement of antibody and antibody fusion protein concentration is vital for process development and manufacturing. Continuous, in-line monitoring of antibody concentration could be useful in a variety of applications, such as controlling the loading of protein A columns to prevent breakthrough, monitoring bioreactor titer, and detecting leaks past ultrafiltration/diafiltration membranes. Molecule-specific
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Oleaginous Yeast Biology Elucidated With Comparative Transcriptomics Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-12-10 Sarah J. Weintraub, Zekun Li, Carter L. Nakagawa, Joseph H. Collins, Eric M. Young
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Monitoring of the Biotechnological Production of Dihydroxyacetone Using a Low-Field 1H NMR Spectrometer Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-12-10 Lukas Mahler, Ebru Tasdemir, Anna Nickisch-Hartfiel, Christian Mayer, Martin Jaeger
The concept of sustainable production necessitates the utilization of waste and by-products as raw materials, the implementation of biotechnological processes, and the introduction of automated real-time monitoring for efficient use of resources. One example is the biocatalyzed conversion of the reusable by-product glycerin by acetic acid bacteria to dihydroxyacetone (DHA), which is of great importance
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Increased Mevalonate Production Using Engineered Citrate Synthase and Phosphofructokinase Variants of Escherichia coli Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-12-09 Jeffrey K. Dodelin, Abigail E. Rose, Hemshikha Rajpurohit, Mark A. Eiteman
Mevalonate is a biochemical precursor to a wide range of isoprenoids. The mevalonate pathway uses three moles of acetyl-CoA, and therefore native pathways which metabolize acetyl-CoA compete with mevalonate synthesis. Moreover, the final step in mevalonate formation, mediated by hydroxymethylglutaryl-CoA reductase, requires NADPH as a co-substrate. This study focuses on chromosomal modification of
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Photoproduction of Aviation Fuel β-Caryophyllene From the Eukaryotic Green Microalga Chlamydomonas reinhardtii Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-12-08 Xiaotan Dou, Mengjie Li, Yunlong Ge, Gerui Yin, Xinyu Wang, Song Xue, Baolin Jia, Lihan Zi, Huihui Wan, Yimei Xi, Zhanyou Chi, Fantao Kong