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Residence time distribution in continuous virus filtration Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-03-17 Yu-Cheng Chen, Gabriele Recanati, Fernando De Mathia, Dong-Qiang Lin, Alois Jungbauer
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Improving stability of human three dimensional skin equivalents using plasma surface treatment Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-03-12 Suphanun Phuphanitcharoenkun, Fiona Louis, Yoshihiro Sowa, Michiya Matsusaki, Tanapat Palaga
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Transport‐controlled growth decoupling for self‐induced protein expression with a glycerol‐repressible genetic circuit Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-03-12 Alvaro R. Lara, Flavio Kunert, Vincent Vandenbroucke, Hilal Taymaz‐Nikerel, Luz María Martínez, Juan‐Carlos Sigala, Frank Delvigne, Guillermo Gosset, Jochen Büchs
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Continuous multi‐column capture of monoclonal antibodies with convective diffusive membrane adsorbers Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-03-12 Fabian Schmitz, Elias Knöchelmann, Thomas Kruse, Mirjana Minceva, Markus Kampmann
Downstream processing is the bottleneck in the continuous manufacturing of monoclonal antibodies (mAbs). To overcome throughput limitations, two different continuous processes with a novel convective diffusive protein A membrane adsorber (MA) were investigated: the rapid cycling parallel multi‐column chromatography (RC‐PMCC) process and the rapid cycling simulated moving bed (RC‐BioSMB) process. First
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Bringing a scalable adaptive hybrid modeling framework closer to industrial use: Application on a multiscale fungal fermentation Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-03-08 Thomas Rydal, Jesper Frandsen, Gisela Nadal‐Rey, Mads Orla Albæk, Pedram Ramin
Digitalization has paved the way for new paradigms such as digital shadows and digital twins for fermentation processes, opening the door for real‐time process monitoring, control, and optimization. With a digital shadow, real‐time model adaptation to accommodate complex metabolic phenomena such as metabolic shifts of a process can be monitored. Despite the many benefits of digitalization, the potential
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Enhancing real‐time cell culture monitoring: Automated Raman model optimization with Taguchi method Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-03-08 Xiaoxiao Dong, Xu Yan, Yuxiang Wan, Dong Gao, Jingyu Jiao, Haibin Wang, Haibin Qu
Raman spectroscopy has found widespread usage in monitoring cell culture processes both in research and practical applications. However, commonly, preprocessing methods, spectral regions, and modeling parameters have been chosen based on experience or trial‐and‐error strategies. These choices can significantly impact the performance of the models. There is an urgent need for a simple, effective, and
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Host cell protein networks as a novel co‐elution mechanism during protein A chromatography Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-03-08 Sherin Panikulam, Alexander Hanke, Frieder Kroener, Anette Karle, Oliver Anderka, Thomas K. Villiger, Nicolas Lebesgue
Host cell proteins (HCPs) are process‐related impurities of therapeutic proteins produced in for example, Chinese hamster ovary (CHO) cells. Protein A affinity chromatography is the initial capture step to purify monoclonal antibodies or Fc‐based proteins and is most effective for HCP removal. Previously proposed mechanisms that contribute to co‐purification of HCPs with the therapeutic protein are
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ASIA: An automated stress‐inducible adaptor for enhanced stress protein expression in engineered Escherichia coli Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-03-07 Chuan‐Chieh Hsiang, I‐Son Ng
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Platform development for high‐throughput optimization of perfusion processes—Part II: Variation of perfusion rate strategies in microwell plates Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-03-04 Marie Dorn, Ciara Lucas, Kerensa Klottrup‐Rees, Ken Lee, Martina Micheletti
The biopharmaceutical industry is replacing fed‐batch with perfusion processes to take advantage of reduced capital and operational costs due to the operation at high cell densities (HCD) and improved productivities. HCDs are achieved by cell retention and continuous medium exchange, which is often based on the cell‐specific perfusion rate (CSPR). To obtain a cost‐productive process the perfusion rate
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Engineering an Escherichia coli based in vivo mRNA manufacturing platform Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-02-29 Edward Curry, George Muir, Jixin Qu, Zoltán Kis, Martyn Hulley, Adam Brown
Synthetic mRNA is currently produced in standardized in vitro transcription systems. However, this one‐size‐fits‐all approach has associated drawbacks in supply chain shortages, high reagent costs, complex product‐related impurity profiles, and limited design options for molecule‐specific optimization of product yield and quality. Herein, we describe for the first time development of an in vivo mRNA
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Correction to “Synergistic co‐utilization of biomass‐derived sugars enhances aromatic amino acid production by engineered Escherichia coli” Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-02-29
Liu, A., Machas, M., Mhatre, A., Hajinajaf, N., Sarnaik, A., Nichols, N., Frazer, S., Wang, X., Varman, A. M., Nielsen, D. R. (2024). Synergistic co-utilization of biomass-derived sugars enhances aromatic amino acid production by engineered Escherichia coli. Biotechnology and Bioengineering, 121:784–794. Throughout the “Results & Discussion” section, the text “Entner-Doudoroff pathway” and the abbreviated
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Simultaneous prediction of 16 quality attributes during protein A chromatography using machine learning based Raman spectroscopy models Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-02-29 Jiarui Wang, Jingyi Chen, Joey Studts, Gang Wang
Several key technologies for advancing biopharmaceutical manufacturing depend on the successful implementation of process analytical technologies that can monitor multiple product quality attributes in a continuous in‐line setting. Raman spectroscopy is an emerging technology in the biopharma industry that promises to fit this strategic need, yet its application is not widespread due to limited success
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Quantitative characterization of tumor cell traction force on extracellular matrix by hydrogel microsphere stress sensor Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-02-26 Xingquan Ma, Cong Wang, Changchun Ji, Xiaoshan Cao, Yuqing Dong
Cell traction force (CTF) is a kind of active force that is a cell senses external environment and actively applies to the contact matrix which is currently a representative stress in cell–extracellular matrix (ECM) interaction. Studying the distribution and variation of CTF during cell–ECM interaction help to explain the impact of physical factors on cell behaviors from the perspective of mechanobiology
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Platform development for high‐throughput optimization of perfusion processes: Part I: Implementation of cell bleeds in microwell plates Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-02-23 Marie Dorn, Kerensa Klottrup‐Rees, Ken Lee, Martina Micheletti
The promise of continuous processing to increase yields and improve product quality of biopharmaceuticals while decreasing the manufacturing footprint is transformative. Developing and optimizing perfusion operations requires screening various parameters, which is expensive and time‐consuming when using benchtop bioreactors. Scale‐down models (SDMs) are the most feasible option for high‐throughput
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NIR spectroscopy—CNN‐enabled chemometrics for multianalyte monitoring in microbial fermentation Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-02-23 Shantanu Banerjee, Shyamapada Mandal, Naveen G. Jesubalan, Rijul Jain, Anurag S. Rathore
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Robust platform for inline Raman monitoring and control of perfusion cell culture Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-02-23 Boyong Wan, Misaal Patel, George Zhou, Michael Olma, Marco Bieri, Marvin Mueller, Emmanuel Appiah‐Amponsah, Bhumit Patel, Karthik Jayapal
Perfusion cell culture has been gaining increasing popularity for biologics manufacturing due to benefits such as smaller footprint, increased productivity, consistent product quality and manufacturing flexibility, cost savings, and so forth. Process Analytics Technologies tools are highly desirable for effective monitoring and control of long‐running perfusion processes. Raman has been widely investigated
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Enhanced sporulation of B. licheniformis BF‐002 through automatic co‐feeding of carbon and nitrogen sources Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-02-21 Jian Ding, Qingyuan Liu, Wenbiao Hou, Jun Cai, Bo Wang, Cheng Lu
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Biotechnology and Bioengineering: Volume 121, Number 3, March 2024 Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-02-19
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Bayesian cell therapy process optimization Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-02-19 Evan Claes, Tommy Heck, Kathleen Coddens, Maarten Sonnaert, Jan Schrooten, Jan Verwaeren
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A robust multinutrient kinetic model for enhanced lutein and biomass yields in mixotrophic microalgae cultivation: A step towards successful large‐scale productions Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-02-19 Jihed Bentahar, Jean‐Sébastien Deschênes
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Contributions of Chinese hamster ovary cell derived extracellular vesicles and other cellular materials to hollow fiber filter fouling during perfusion manufacturing of monoclonal antibodies Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-02-19 Yixiao Zhang, Sri Madabhushi, Tiffany Tang, Hassan Raza, David J. Busch, Xi Zhao, James Ormes, Sen Xu, Joseph Moroney, Rubin Jiang, Henry Lin, Ren Liu
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An optimized polymeric delivery system for piggyBac transposition Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-02-19 Daniel Nisakar Meenakshi Sundaram, Remant Bahadur K. C., Wei Fu, Hasan Uludağ
The piggyBac transposon/transposase system has been explored for long‐term, stable gene expression to execute genomic integration of therapeutic genes, thus emerging as a strong alternative to viral transduction. Most studies with piggyBac transposition have employed physical methods for successful delivery of the necessary components of the piggyBac system into the cells. Very few studies have explored
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Supervised and unsupervised machine learning approaches for monitoring subvisible particles within an aluminum‐salt adjuanted vaccine formulation Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-02-19 David N. Greenblott, Caitlin V. Wood, Jingtao Zhang, Nelia Viza, Ramesh Chintala, Christopher P. Calderon, Theodore W. Randolph
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A low-temperature SPR-based assay for monoclonal antibody galactosylation and fucosylation assessment using FcγRIIA/B Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-02-14 Jimmy Gaudreault, Catherine Forest-Nault, Michel Gilbert, Yves Durocher, Olivier Henry, Gregory De Crescenzo
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Selective hydrophobic interaction chromatography for high purity of supercoiled DNA plasmids Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-02-14 Klemen Božič, Ajda Sedlar, Špela Kralj, Urh Černigoj, Aleš Štrancar, Rok Sekirnik
High purity of plasmid DNA (pDNA), particularly in supercoiled isoform (SC), is used for various biopharmaceutical applications, such as a transfecting agent for production of gene therapy viral vectors, for pDNA vaccines, or as a precursor for linearized form that serves as a template for mRNA synthesis. In clinical manufacturing, pDNA is commonly extracted from Escherichia coli cells with alkaline
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New insights into Chlorella vulgaris applications Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-02-11 Mohammed Al-Hammadi, Mine Güngörmüşler
Environmental pollution is a big challenge that has been faced by humans in contemporary life. In this context, fossil fuel, cement production, and plastic waste pose a direct threat to the environment and biodiversity. One of the prominent solutions is the use of renewable sources, and different organisms to valorize wastes into green energy and bioplastics such as polylactic acid. Chlorella vulgaris
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Deep hybrid modeling of a HEK293 process: Combining long short-term memory networks with first principles equations Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-02-11 João R. C. Ramos, José Pinto, Gil Poiares-Oliveira, Ludovic Peeters, Patrick Dumas, Rui Oliveira
The combination of physical equations with deep learning is becoming a promising methodology for bioprocess digitalization. In this paper, we investigate for the first time the combination of long short-term memory (LSTM) networks with first principles equations in a hybrid workflow to describe human embryonic kidney 293 (HEK293) culture dynamics. Experimental data of 27 extracellular state variables
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Accelerating attribute-focused process and product development through the development and deployment of autonomous process analytical technology platform system Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-02-08 Chao-Hsiang (Richard) Wu, Becky Chan, Zac Sarich, Yaokai Duan, Janice Chen, Jiu-Li Song, Mike Berke, Les P. Miranda, Chetan T. Goudar
Enabling real-time monitoring and control of the biomanufacturing processes through product quality insights continues to be an area of focus in the biopharmaceutical industry. The goal is to manufacture products with the desired quality attributes. To realize this rigorous attribute-focused Quality by Design approach, it is critical to support the development of processes that consistently deliver
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Nanopore sequencing improves construction of customized CRISPR-based gene activation libraries Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-01-31 Handing Wang, Heng Yih Tan, Jiazhang Lian, Kang Zhou
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Cell culture product quality attribute prediction using convolutional neural networks and Raman spectroscopy Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-01-29 Hamid Khodabandehlou, Mohammad Rashedi, Tony Wang, Aditya Tulsyan, Gregg Schorner, Christopher Garvin, Cenk Undey
Advanced process control in the biopharmaceutical industry often lacks real-time measurements due to resource constraints. Raman spectroscopy and Partial Least Squares (PLS) models are often used to monitor bioprocess cultures in real-time. In spite of the ease of training, the accuracy of the PLS model is impacted if it is not used to predict quality attributes for the cell lines it is trained on
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A novel experimental method to determine substrate uptake kinetics of gaseous substrates applied to the carbon monoxide-fermenting Clostridium autoethanogenum Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-01-24 Maximilienne T. Allaart, Charilaos Korkontzelos, Diana Z. Sousa, Robbert Kleerebezem
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Computer-directed rational design enhanced the thermostability of carbonyl reductase LsCR for the synthesis of ticagrelor precursor Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-01-24 Shen-Yuan Xu, Rong-Liang Chu, Hua-Tao Liu, Chun-Yue Weng, Ya-Jun Wang, Yu-Guo Zheng
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Giving the cells what they need when they need it: Biosensor-based feeding control Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-01-23 Romain Kinet, Anne Richelle, Michael Colle, Didier Demaegd, Moritz von Stosch, Matthew Sanders, Hannah Sehrt, Frank Delvigne, Philippe Goffin
“Giving the cells exactly what they need, when they need it” is the core idea behind the proposed bioprocess control strategy: operating bioprocess based on the physiological behavior of the microbial population rather than exclusive monitoring of environmental parameters. We are envisioning to achieve this through the use of genetically encoded biosensors combined with online flow cytometry (FCM)
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Genetic algorithm-based semisupervised convolutional neural network for real-time monitoring of Escherichia coli fermentation of recombinant protein production using a Raman sensor Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-01-22 Yuan Liu, Xiaotian Zhou, Teng Wang, An Luo, Zhaojun Jia, Xingquan Pan, Weiqi Cai, Mengge Sun, Xuezhong Wang, Zhenguo Wen, Guangzheng Zhou
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Factors affecting product association as a mechanism of host-cell protein persistence in bioprocessing Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-01-19 Young Hoon Oh, Matthew L. Becker, Kerri M. Mendola, Leila H. Choe, Lie Min, Kelvin H. Lee, Yinges Yigzaw, Alexander Seay, Jerome Bill, Xuanwen Li, David J. Roush, Steven M. Cramer, Stefano Menegatti, Abraham M. Lenhoff
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Engineering of a decellularized bovine skin coated with antibiotics-loaded electrospun fibers with synergistic antibacterial activity for the treatment of infectious wounds Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-01-17 Sanaz Alizadeh, Jila Majidi, Mozhgan Jahani, Zahra Esmaeili, Zeinab Nokhbedehghan, Zahra Aliakbar Ahovan, Hajar Nasiri, Amir Mellati, Ali Hashemi, Narendra Pal Singh Chauhan, Mazaher Gholipourmalekabadi
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Acoustic levitation as a tool for cell-driven self-organization of human cell spheroids during long-term 3D culture Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-01-15 Lucile Rabiet, Lousineh Arakelian, Nathan Jeger-Madiot, Duván Rojas García, Jérôme Larghero, Jean-Luc Aider
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Realization of digital twin for dynamic control toward sample variation of ion exchange chromatography in antibody separation Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-01-17 Ce Shi, Xu-Jun Chen, Xue-Zhao Zhong, Yan Yang, Dong-Qiang Lin, Ran Chen
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Cover Image, Volume 121, Number 2, February 2024 Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-01-14 Oliver McRae, Peter L. L. Walls, Venkatesh Natarajan, Chris Antoniou, James C. Bird
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Contactless face video based vital signs detection framework for continuous health monitoring using feature optimization and hybrid neural network Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-01-14 Anju Anil Jalaja, Maruthai Kavitha
Continuous monitoring of vital signs such as respiration and heart rate is essential to detect and predict conditions that may affect the patient's well-being. To detect these vital signs most medical systems use contact sensors. They are not feasible for long term monitoring and are not repeatable. Vital signs using facial video-noncontact monitoring are becoming increasingly important. Researchers
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Dynamic flux balance analysis of high cell density fed-batch culture of Escherichia coli BL21 (DE3) with mass spectrometry-based spent media analysis Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-01-12 Hardik Dodia, Vivek Mishra, Prajval Nakrani, Charandatta Muddana, Anant Kedia, Sneha Rana, Deepti Sahasrabuddhe, Pramod P. Wangikar
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Reviewing the process intensification landscape through the introduction of a novel, multitiered classification for downstream processing Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-01-12 Louis Crowley, Paul Cashen, Marc Noverraz, Martin Lobedann, Piergiuseppe Nestola
A demand for process intensification in biomanufacturing has increased over the past decade due to the ever-expanding market for biopharmaceuticals. This is largely driven by factors such as a surge in biosimilars as patents expire, an aging population, and a rise in chronic diseases. With these market demands, pressure upon biomanufacturers to produce quality products with rapid turnaround escalates
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Mimicking CHO large-scale effects in the single multicompartment bioreactor: A new approach to access scale-up behavior Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-01-08 Lena Gaugler, Sebastian Hofmann, Michael Schlüter, Ralf Takors
During the scale-up of biopharmaceutical production processes, insufficiently predictable performance losses may occur alongside gradients and heterogeneities. To overcome such performance losses, tools are required to explain, predict, and ultimately prohibit inconsistencies between laboratory and commercial scale. In this work, we performed CHO fed-batch cultivations in the single multicompartment
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Mechanobiological stimulation in organ-on-a-chip systems reduces hepatic drug metabolic capacity in favor of regenerative specialization Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-01-07 Benedikt Scheidecker, Stéphane Poulain, Masahiro Sugimoto, Hiroshi Arakawa, Soo H. Kim, Takumi Kawanishi, Yukio Kato, Mathieu Danoy, Masaki Nishikawa, Yasuyuki Sakai
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A highly efficient cell culture method using oxygen-permeable PDMS-based honeycomb microwells produces functional liver organoids from human induced pluripotent stem cell-derived carboxypeptidase M liver progenitor cells Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-01-07 Tia Utami, Mathieu Danoy, Rubina Rahaman Khadim, Fumiya Tokito, Hiroshi Arakawa, Yukio Kato, Taketomo Kido, Atsushi Miyajima, Masaki Nishikawa, Yasuyuki Sakai
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Engineering Escherichia coli for constitutive production of monophosphoryl lipid A vaccine adjuvant Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-01-07 Hyunjung Jin, Yuhyun Ji, Jinsu An, Da Hui Ha, Ye-Ram Lee, Hye-Ji Kim, Choon Geun Lee, Wooyeon Jeong, Ick Chan Kwon, Eun Gyeong Yang, Ki Hun Kim, Chankyu Lee, Hak Suk Chung
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Recombinant protein expression in proteome-reduced cells under aerobic and oxygen-limited regimes Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-01-04 Alvaro R. Lara, Jose Utrilla, Luz María Martínez, Niels Krausch, Lucas Kaspersetz, David Hidalgo, Nicolas Cruz-Bournazou, Peter Neubauer, Juan-Carlos Sigala, Guillermo Gosset, Jochen Büchs
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First- and second-generation integrated process for bioethanol production: Fermentation of molasses diluted with hemicellulose hydrolysate by recombinant Saccharomyces cerevisiae Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-01-04 Isabela de Oliveira Pereira, Ângela A. dos Santos, Nick C. Guimarães, Cleilton S. Lima, Eduardo Zanella, Akinori Matsushika, Sarita C. Rabelo, Boris U. Stambuk, Jaciane L. Ienczak
The integration of first- (1G) and second-generation (2G) ethanol production by adding sugarcane juice or molasses to lignocellulosic hydrolysates offers the possibility to overcome the problem of inhibitors (acetic acid, furfural, hydroxymethylfurfural and phenolic compounds), and add nutrients (such as salts, sugars and nitrogen sources) to the fermentation medium, allowing the production of higher
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Protein constraints in genome-scale metabolic models: Data integration, parameter estimation, and prediction of metabolic phenotypes Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-01-04 Maurício Alexander de Moura Ferreira, Wendel Batista da Silveira, Zoran Nikoloski
Genome-scale metabolic models provide a valuable resource to study metabolism and cell physiology. These models are employed with approaches from the constraint-based modeling framework to predict metabolic and physiological phenotypes. The prediction performance of genome-scale metabolic models can be improved by including protein constraints. The resulting protein-constrained models consider data
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Identification of yttrium oxide-specific peptides for future recycling of rare earth elements from electronic scrap Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-01-02 Danielle Maass, Peter Boelens, Christoph Bloss, Gerda Claus, Sonja Harter, Dominik Günther, Katrin Pollmann, Franziska Lederer
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Biodiesel production in India: Prospects, challenges, and sustainable directions Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-01-02 Christopher Selvam Damian, Yuvarajan Devarajan, Ravikumar Jayabal
Biodiesel has the potential to serve as a feasible substitute for fossil fuels in many sectors, including transportation and internal combustion engines, without requiring extensive modifications. UsinHemg alternative energy sources, including biodiesel, is necessary to effectively tackle the growing demand predominantly observed in the transportation sector. This review is aimed to examine the technological
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2,6-diaminopurine (Z)-containing toehold probes improve genotyping sensitivity Biotechnol. Bioeng. (IF 3.8) Pub Date : 2023-12-27 Shaohua Kang, Qian Liu, Jie Zhang, Yan Zhang, Hao Qi
2,6-diaminopurine (Z), a naturally occurring noncanonical nucleotide base found in bacteriophages, enhances DNA hybridization by forming three hydrogen bonds with thymine (T). These distinct biochemical characteristics make it particularly valuable in applications that rely on the thermodynamics of DNA hybridization. However, the practical use of Z-containing oligos is limited by their high production
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Investigation into struvite precipitation: A commonly encountered problem during fermentations on chemically defined media Biotechnol. Bioeng. (IF 3.8) Pub Date : 2023-12-27 Thomas Steimann, David Wollborn, Florian Röck, Rebekka Horstmann, Elina Schmitt, Jonas Johannes Christ, Lars Mathias Blank, Jochen Büchs
Chemically defined mineral media are widely used in bioprocesses, as these show less batch to batch variation compared with complex media. Nonetheless, the recommended media formulations often lead to the formation of precipitants at elevated pH values. These precipitates are insoluble and reduce the availability of macronutrients to the cells, which can result in limiting growth rates and lower productivity
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Telehealth of cardiac devices for CVD treatment Biotechnol. Bioeng. (IF 3.8) Pub Date : 2023-12-27 Sushil Shelke, Krishna Veerubhotla, Yugyung Lee, Chi H. Lee
This review covers currently available cardiac implantable electronic devices (CIEDs) as well as updated progress in real-time monitoring techniques for CIEDs. A variety of implantable and wearable devices that can diagnose and monitor patients with cardiovascular diseases are summarized, and various working mechanisms and principles of monitoring techniques for Telehealth and mHealth are discussed
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Concentration of clarified pool by single-pass tangential flow filtration to improve productivity of protein A capture step: Impact of clarification strategies Biotechnol. Bioeng. (IF 3.8) Pub Date : 2023-12-27 Santosh B. Rahane, Akshat Gupta, Philip Szymanski, Dana Kinzlmaier, Patrick McGee, Elizabeth Goodrich
Protein A capture chromatography remains a high-cost and relatively low-productivity step for downstream processing of monoclonal antibodies. As bioprocessing transitions toward intensified processes, maximizing the efficiency of individual steps is key to achieving economic targets. This study was performed to assess the impact of inline concentration of clarified cell culture fluid (CCF), using single-pass
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PETR: A novel peristaltic mixed tubular bioreactor simulating human colonic conditions Biotechnol. Bioeng. (IF 3.8) Pub Date : 2023-12-27 David Vorländer, Gábor Schultz, Kristin Hoffmann, Detlev Rasch, Katrin Dohnt
A novel bioreactor simulating human colonic conditions for in vitro cultivation of intestinal microbiota is presented. The PEristaltic mixed Tubular bioReactor (PETR) is modular designed and periodically kneaded to simulate intestinal peristalsis. The reactor is introduced, characterized from a bioprocess engineer's perspective and discussed in its ability to mimic colon conditions. PETR provides physiological
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Humanized mouse models: A valuable platform for preclinical evaluation of human cancer Biotechnol. Bioeng. (IF 3.8) Pub Date : 2023-12-27 Yuening Yang, Jiaqian Li, Dan Li, Weilin Zhou, Feiyang Yan, Wei Wang
Animal models are routinely employed to assess the treatments for human cancer. However, due to significant differences in genetic backgrounds, traditional animal models are unable to meet bioresearch needs. To overcome this restriction, researchers have generated and optimized immunodeficient mice, and then engrafted human genes, cells, tissues, or organs in mice so that the responses in the model
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Modeling large-scale bioreactors with diffusion equations. Part I: Predicting axial dispersion coefficient and mixing times Biotechnol. Bioeng. (IF 3.8) Pub Date : 2023-12-27 Pauli Losoi, Jukka Konttinen, Ville Santala
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Modeling large-scale bioreactors with diffusion equations. Part II: Characterizing substrate, oxygen, temperature, carbon dioxide, and pH profiles Biotechnol. Bioeng. (IF 3.8) Pub Date : 2023-12-27 Pauli Losoi, Jukka Konttinen, Ville Santala
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Chromosome recombination and modification by LoxP-mediated evolution in Vibrio natriegens using CRISPR-associated transposases Biotechnol. Bioeng. (IF 3.8) Pub Date : 2023-12-22 Jiaqi Xu, Yijie Sun, Jianping Wu, Sheng Yang, Lirong Yang
Chromosome rearrangement by LoxP-mediated evolution has emerged as a powerful approach to studying how chromosome architecture impacts phenotypes. However, it relies on the in vitro synthesis of artificial chromosomes. The recently reported CRISPR-associated transposases (CASTs) held great promise for the efficient insertion of abundant LoxP sites directly onto the genome of wild-type strains. In this