The complexity of mechanical and biological processes in filamentous fungal fermentation remains a major obstacle to improving product yield. We previously demonstrated that the AGΔ‐GAGΔ strain of Aspergillus oryzae, lacking both α‐1,3‐glucan (AG) and galactosaminogalactan (GAG), had improved hyphal dispersion, reduced culture viscosity, and increased recombinant protein production. Here, we applied

Environmental pH plays a crucial role in microbial metabolism. Microorganisms adapt their metabolic strategies in response to different pH conditions, which must be carefully controlled in industrial production processes to achieve desired outcomes. However, the dynamic impact of pH on hierarchical utilization of mixed carbon sources remains poorly understood. In this study, we observed that Bacillus

A pH‐dependent affinity complex titration isotherm is derived based on the stoichiometry of target binding and both target and ligand titration equilibria to facilitate mechanistic modeling for protein A chromatography. The final isotherm can be regarded as a direct modification of the Langmuir isotherm with an apparent capacity and equilibrium constant. The model parameters can be estimated from four

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Recombinant vesicular stomatitis virus (rVSV) pseudoparticles displaying foreign glycoproteins are valuable for various applications, including vaccine vectors, oncolytic viruses, and research tools. Replication incompetent rVSV pseudoparticles, which do not encode their envelope proteins necessary for infection, offer increased versatility and rapid manufacturing. Traditionally, pseudotyping has been

Real‐time multivariate statistical process monitoring (RT‐MSPM) is essential to monitor health of bio‐pharmaceutical processes and detect anomalies and faults early in the process. RT‐MSPM methods are commonly used to monitor cell culture process operations in biologics drug substance manufacturing. Batch evolution models (BEMs) are among common RT‐MSPM methods. As an alternative to BEMs, it is possible

There is increasing interest in the development of intensified processes that can provide significant reductions in manufacturing costs for monoclonal antibody (mAb) products. This study examines the performance of an integrated membrane process that combines single‐pass tangential flow filtration (SPTFF) with high‐performance countercurrent membrane purification (HPCMP) for the preconditioning of

Technologies for large‐scale manufacturing of viral vectors for gene therapies, such as tangential flow filtration and membrane chromatography, are under development. In these early stages of process development, techno‐economic analyses are useful for identifying membrane properties yielding the greatest impact on process performance. In this study, we adapted a techno‐economic framework used for

Hyaline cartilage is a dense avascular tissue with low regenerative potential, present at the ends of the diarthrodial joints and in the cartilage growth plate. Skeletal diseases often result from extracellular changes in this tissue; however, studies of these are hindered by the tissue complexity, the difficulty in obtaining human material, and the cost of generating animal models. Recent developments

The gut microbiota plays an essential role in host health by regulating gut barrier function and immune system homeostasis. However, research into the physiological and immunological functions of the gut microbiota using In Vitro models that mimic the immune environment of the gut remains limited. Herein, we developed the Gut Microbial Immune & Epithelial Cellular System (GutMICS), a device for coculturing

Komagataella phaffii, also known as Pichia pastoris, is a powerful host for recombinant protein production, in part due to its exceptionally strong and tightly controlled PAOX1 promoter. Most K. phaffii bioprocesses for recombinant protein production rely on PAOX1 to achieve dynamic control in two‐phase processes. Cells are first grown under conditions that repress PAOX1 (growth phase), followed by

The increasing nitrogen emissions from industrial and agricultural activities have exacerbated pollution in aquatic ecosystems, highlighting a great need for effective nitrogen removal strategies. Genetic engineering has proven to be an effective strategy to improve the nitrogen removal capacity of denitrifying bacteria. However, the widespread use of antibiotics in the genetic modification of denitrifying

Hydrogel scaffolds show high potential in tissue engineering due to excellent mechanical properties and biocompatibility. However, an inherent lack of conductivity limits its application in areas requiring electrical stimulation. To address this issue, chitosan (CS)/gelatin (Gel) scaffolds were prepared with various concentrations of multi‐walled carbon nanotubes (MWCNTs). Results indicated that MWCNT

Removal of host cell components is a significant cost driver in the production of live virus vaccines. Filtration processes such as tangential flow filtration can be effective in this capacity by leveraging the relative size difference between viral particles and host proteins; however, filtration membranes can be fouled by larger proteins, particularly those of the extracellular matrix. In this study

A viral contamination model for steady‐state perfusion cell culture was developed to assess how sampling frequency and volume impacted the expected downstream viral clearance factor in integrated continuous biomanufacturing processes. The model used population balance rate equations for cells and free virions, incorporating the virus infection cycle. It simulated the states of cell cultures for both

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A genetic algorithm‐optimized neural network (ANN‐GA) was developed for real‐time monitoring of gibberellin (GA3) production during Fusarium fujikuroi fermentation. This model addresses the limitations of traditional off‐line detection methods, such as contamination risks and delayed feedback, by integrating six critical inputs—initial glucose concentration, fermentation time, temperature, pH, dissolved

In the published article, the images in Figure 4B,D were found to be from the same sample that was sequentially exposed to different conditions and analyzed. The authors have data from an independent experiment in which the gels were not sequentially analyzed (Figure 4 below). The updated figure does not alter the interpretation of the data or the conclusions of the study. We apologize for any inconvenience

Long‐term stable operation of bioelectrochemical systems (BES) presupposes the avoidance of mass transfer limitations of the electroactive biofilm. Excessive pH‐gradients from bulk to electrode interface or substrate limitations of the electroactive biofilm are known to diminish the electrical performance of BES. In this study the impact of the morphology of a mixed‐species electroactive biofilm cultivated

The reverse adipate degradation pathway (RADP) for adipic acid synthesis has garnered significant interest. However, the limited efficiency of existing pathways and their dependence on plasmids have hindered the practical application of microbial cell factories. In this study, the efficiency of the adipic acid synthetic pathway was enhanced by substituting and combinatorially expressing RADP enzymes

The SARS‐CoV‐2 pandemic highlighted the urgent need for biomanufacturing paradigms that are robust and fast. Here, we demonstrate the rapid process development and scalable cell‐free production of T7 RNA polymerase, a critical component in mRNA vaccine synthesis. We carry out a 1‐L cell‐free gene expression (CFE) reaction that achieves over 90% purity, low endotoxin levels, and enhanced activity relative

The rise in chronic lung diseases globally and the corresponding lag in drug discovery in this field highlights the need for In Vitro models closely mimicking In Vivo lung tissue. Efforts to date have largely focused on In Vitro coculture models, often neglecting the pulmonary vasculature's role in lung physiology and lacking perfusability. To address this gap, we utilized digital light processing

Cranial bone defects from trauma, congenital conditions, or surgery are challenging to treat due to the skull's limited regeneration. Traditional methods like autografts and allografts have drawbacks, including donor site issues and poor integration. 3D‐printed scaffolds provide a patient‐specific alternative, improving bone regeneration and integration. This review evaluates advancements in 3D‐printed

L-2-Aminobutyric acid (L-2-ABA) is a nonnatural chiral α-amino acid which is widely used in various chiral pharmaceuticals and medical intermediates. Currently, the microbial metabolic engineering approach to enable Escherichia coli to produce L-2-ABA autonomously exists the problem of low synthesis efficiency, limiting its large-scale application. In this study, we successfully constructed a strain

Clostridium tyrobutyricum has emerged as a non-pathogenic microbial cell factory capable of anaerobic production of various value-added products, such as butyrate, butanol, and butyl butyrate. This study reports the first systematic engineering of C. tyrobutyricum for the heterologous production of 1,3-propanediol (1,3-PDO) from industrial by-product crude glycerol. Initially, the glycerol reductive

Y. Du, M. Ghodousi, H. Qi, N. Haas, W. Xiao, and A. Khademhosseini, “Sequential Assembly of Cell-Laden Hydrogel Constructs to Engineer Vascular-Like Microchannels,” Biotechnology and Bioengineering 108, no. 7 (2011): 1693–1703, 10.1002/bit.23102. This correction is published due to concerns raised by a third party regarding high similarity between the cell-laden microgels between Figure 4B,D. The authors

Dietrich, A., Heim, L. and Hubbuch, J. (2025), Dual-Stage Cross-Flow Filtration: Integrated Capture and Purification of Virus-Like Particles. Biotechnology and Bioengineering 122: 884–894. https://doi.org/10.1002/bit.28914 In the sections “Abstract”, “Introduction”, “Results”, “Discussion”, and “Conclusion”, as well as in the Graphical Abstract, Figure 1, the captions of Figures 1 and 2, and the supplementary