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Improved Mixing Properties of Stirred Fermentation of an Aspergillus oryzae Hyphal Dispersion Mutant Biotechnol. Bioeng. (IF 3.6) Pub Date : 2025-06-21 Shunya Susukida, Ken Miyazawa, Hikaru Ichikawa, Kiyoaki Muto, Akira Yoshimi, Toshitaka Kumagai, Yoshikazu Kato, Keietsu Abe
The complexity of mechanical and biological processes in filamentous fungal fermentation remains a major obstacle to improving product yield. We previously demonstrated that the AGΔ‐GAGΔ strain of Aspergillus oryzae, lacking both α‐1,3‐glucan (AG) and galactosaminogalactan (GAG), had improved hyphal dispersion, reduced culture viscosity, and increased recombinant protein production. Here, we applied
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Assessment of Anticancer Effects of Aloe vera on 3D Liver Tumor Spheroids in a Microfluidic Platform Biotechnol. Bioeng. (IF 3.6) Pub Date : 2025-06-20 Atakan Tevlek, Gunes Kibar, Barbaros Cetin
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The P134Q Sucrose Phosphorylase: Subtle Changes in the Catalytic Properties Benefit the Production of 2‐O‐α‐Glucosyl Glycerol Biotechnol. Bioeng. (IF 3.6) Pub Date : 2025-06-20 Alexander Sigg, Mario Klimacek, Martin Pfeiffer, Jorick Franceus, Tom Desmet, Bernd Nidetzky
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3D Culture in Functionalized FN‐Silk Networks Facilitate Proliferation, Differentiation and Phenotypic Stability of Mature Human Primary Cells and Stem Cells Biotechnol. Bioeng. (IF 3.6) Pub Date : 2025-06-17 Astrid Källén, Nayere Taebnia, Mona Widhe, Volker M. Lauschke, My Hedhammar
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Unsupervised Machine Learning‐Based Process Analytical Tools for Near Real‐Time Cell Morphology Analysis During CAR‐T Cell Manufacturing Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-06-16 Nidhi G. Thite, Michael Yarnell, Terry J. Fry, Matthew Seefeldt, Christopher P. Calderon, Theodore W. Randolph
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Systems Biology Analysis of the Effect of pH on Lactate Fermentation in Bacillus coagulans Under Mixed Carbon Sources Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-06-12 Zhihao Liu, Yonghong Wang
Environmental pH plays a crucial role in microbial metabolism. Microorganisms adapt their metabolic strategies in response to different pH conditions, which must be carefully controlled in industrial production processes to achieve desired outcomes. However, the dynamic impact of pH on hierarchical utilization of mixed carbon sources remains poorly understood. In this study, we observed that Bacillus
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An Affinity Complex Titration Isotherm for Mechanistic Modeling in Protein: A Chromatography Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-06-12 Wendi Zhang, Virginia DiNenna, Todd Przybycien
A pH‐dependent affinity complex titration isotherm is derived based on the stoichiometry of target binding and both target and ligand titration equilibria to facilitate mechanistic modeling for protein A chromatography. The final isotherm can be regarded as a direct modification of the Langmuir isotherm with an apparent capacity and equilibrium constant. The model parameters can be estimated from four
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Biotechnology and Bioengineering: Volume 122, Number 7, July 2025 Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-06-12
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Data‐Augmented Deep Learning Algorithm for Accurate Control of Bioethanol Fermentation Using an Online Raman Analyzer Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-06-09 Kaidi Ji, Xiaofei Yu, Lifan Chen, Yongbo Wang, Zhiqiang Guo, Biao Chen, Qingyang Li, Zhen Li, Hu Zhang, Guan Wang, Yingping Zhuang, Yinlan Ruan
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Rewiring Cupriavidus necator for Enhanced Polyhydroxybutyrate Production via Genetic Toolkits and Feeding Strategy Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-06-07 Yu‐Chieh Lin, I‐Son Ng
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Enrichment of Full AAV2 Using Multicolumn Countercurrent Solvent Gradient Purification (MCSGP) Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-06-05 Julia M. Müller, Tereza Müllerová, Daniela Tobler, Damian Hauri, Richard Plieninger, Yuki Higuchi, Ryosuke Takahashi, Sebastian Vogg, Thomas Müller‐Späth, Thomas K. Villiger
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Rational Design and Protein Engineering of {SH2 Domain–≫ Flexible Linker–≫ Self‐Controlling Peptide} Fusion System With Phosphorylation‐Regulated Molecular Switch Functionality Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-06-05 Peng Zhou, Yunyi Zhang, Kexin Li, Haiyang Ye, Li Mei, Shuyong Shang
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Scalable Production of Recombinant Vesicular Stomatitis Virus Pseudoparticles Using HEK293 Suspension Cultures Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-06-04 Zhe Zhang, Elzbieta Wloga, Benjamin O. Fulton, Louis Coplan, Hanne Bak, Andrew D. Tustian
Recombinant vesicular stomatitis virus (rVSV) pseudoparticles displaying foreign glycoproteins are valuable for various applications, including vaccine vectors, oncolytic viruses, and research tools. Replication incompetent rVSV pseudoparticles, which do not encode their envelope proteins necessary for infection, offer increased versatility and rapid manufacturing. Traditionally, pseudotyping has been
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A Scalable Deep Learning Approach for Real‐Time Multivariate Monitoring of Biopharmaceutical Processes With No Prior Product‐Specific History Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-06-03 Nima Sammaknejad, Jessica Lee, Jan Michael Austria, Nadia Duenas, Leila Heiba, Govi Sridharan, Jeff Davis, Cenk Undey
Real‐time multivariate statistical process monitoring (RT‐MSPM) is essential to monitor health of bio‐pharmaceutical processes and detect anomalies and faults early in the process. RT‐MSPM methods are commonly used to monitor cell culture process operations in biologics drug substance manufacturing. Batch evolution models (BEMs) are among common RT‐MSPM methods. As an alternative to BEMs, it is possible
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Integration of Single Pass Tangential Flow Filtration and High Performance Countercurrent Membrane Purification for Intensification of Monoclonal Antibody Processing Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-06-02 Mirko Minervini, Aylin Mohammadzadehmarandi, Ali Behboudi, Sheldon F. Oppenheim, Andrew L. Zydney
There is increasing interest in the development of intensified processes that can provide significant reductions in manufacturing costs for monoclonal antibody (mAb) products. This study examines the performance of an integrated membrane process that combines single‐pass tangential flow filtration (SPTFF) with high‐performance countercurrent membrane purification (HPCMP) for the preconditioning of
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Dynamic Regulation of Efflux Pump Protein AcrA by Quorum Sensing System to Improve Free Fatty Acids Production in Escherichia coli Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-05-30 Lixia Fang, Peishi Wen, Jiaqi Zhang, Yingxiu Cao
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Techno‐Economic Analysis of Membrane‐Based Purification Platforms for AAV Vector Production Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-05-30 Juan J. Romero, Eleanor W. Jenkins, Jacob I. Monroe, S. Ranil Wickramasinghe, Xianghong Qian, Dibakar Bhattacharyya, Scott M. Husson
Technologies for large‐scale manufacturing of viral vectors for gene therapies, such as tangential flow filtration and membrane chromatography, are under development. In these early stages of process development, techno‐economic analyses are useful for identifying membrane properties yielding the greatest impact on process performance. In this study, we adapted a techno‐economic framework used for
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N‐ to C‐Glycoside Rearrangement of Uridine 5′‐Phosphate in Two Enzymatic Steps for the Production of Pseudouridine 5′‐Phosphate Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-05-28 Martin Pfeiffer, Franziska Guld, Bernd Nidetzky
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Dynamic Compression Improves Chondrogenesis in the Tissue Engineered Model of Cartilage Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-05-26 Marc V. Farcasanu, Thais de las Heras Ruiz, Francesca M. Johnson de Sousa Brito, Jamie Soul, Jonathan Coxhead, Matthew J. German, David A. Young, Ana M. Ferreira‐Duarte, Katarzyna A. Piróg
Hyaline cartilage is a dense avascular tissue with low regenerative potential, present at the ends of the diarthrodial joints and in the cartilage growth plate. Skeletal diseases often result from extracellular changes in this tissue; however, studies of these are hindered by the tissue complexity, the difficulty in obtaining human material, and the cost of generating animal models. Recent developments
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Post‐Treatment of CO₂ Emissions With Microalgae: Magnetic Field‐Induced Improvements in an AirLift Photoreactor Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-05-26 Luis Torres, José Octavio Saucedo‐Lucero, Aitor Aizpuru, Sonia Arriaga
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Development of the Gut Microbial Immune and Epithelial Cellular System (GutMICS) to Investigate the Immunological Role of Gut Anaerobes Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-05-24 Seo‐Young Kwon, Sung‐Hyun Jo, Joonha Park, Ji‐Hyeon Park, Ye‐Rim Kim, Ji‐Hyun Baek, Min‐Gyu Kim, Bo‐Gyeong Choi, Na Youn Hong, Hee Kyoung Jung, Hee‐Wook Ryu, Jessie S. Jeon, Yun‐Gon Kim
The gut microbiota plays an essential role in host health by regulating gut barrier function and immune system homeostasis. However, research into the physiological and immunological functions of the gut microbiota using In Vitro models that mimic the immune environment of the gut remains limited. Herein, we developed the Gut Microbial Immune & Epithelial Cellular System (GutMICS), a device for coculturing
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Balancing Doses of EL222 and Light Improves Optogenetic Induction of Protein Production in Komagataella phaffii Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-05-24 Shannon M. Hoffman, Sebastián Espinel‐Ríos, Sarah K. Kwartler, Makoto A. Lalwani, José L. Avalos
Komagataella phaffii, also known as Pichia pastoris, is a powerful host for recombinant protein production, in part due to its exceptionally strong and tightly controlled PAOX1 promoter. Most K. phaffii bioprocesses for recombinant protein production rely on PAOX1 to achieve dynamic control in two‐phase processes. Cells are first grown under conditions that repress PAOX1 (growth phase), followed by
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Controlling Human Stem Cell‐Derived Islet Composition Using Magnetic Sorting Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-05-22 Allison B. Kelley, Mira Shunkarova, Marlie M. Maestas, Sarah E. Gale, Noyonika Mukherjee, Nathaniel J. Hogrebe, Jeffrey R. Millman
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Enhancing Biological Denitrification Efficiency With a Novel Antibiotic‐Free Plasmid Strategy Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-05-21 Yang‐Yang Fan, Yu‐Han Chai, Jie‐Ru Peng, Xin‐Lu Cai, Juan‐Juan Zou, Min Wang, Li Zhang, Wen‐Wen Liu, Xiang Xiao, Han‐Qing Yu
The increasing nitrogen emissions from industrial and agricultural activities have exacerbated pollution in aquatic ecosystems, highlighting a great need for effective nitrogen removal strategies. Genetic engineering has proven to be an effective strategy to improve the nitrogen removal capacity of denitrifying bacteria. However, the widespread use of antibiotics in the genetic modification of denitrifying
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CS/Gel/MWCNTs Conductive Scaffolds Assisted by Electrical Stimulus for Skin Tissue Engineering Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-05-16 Zixian Liu, Zijun Ma, Nini Li, Jinyao Zhang, Meng Li, Lu Han, Rong Cheng, Zhizhong Shen, Dan Han, Shengbo Sang
Hydrogel scaffolds show high potential in tissue engineering due to excellent mechanical properties and biocompatibility. However, an inherent lack of conductivity limits its application in areas requiring electrical stimulation. To address this issue, chitosan (CS)/gelatin (Gel) scaffolds were prepared with various concentrations of multi‐walled carbon nanotubes (MWCNTs). Results indicated that MWCNT
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Modeling and Optimization of Recombinant Tocilizumab Production From Pichia pastoris Using Response Surface Methodology and Artificial Neural Network Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-05-15 Prabir Kumar Das, Ansuman Sahoo, Venkata Dasu Veeranki
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CRISPR‐Editing of the Vero Cell Line Improves Processability of Live Virus Vaccines by Enabling Targeted Proteolysis of Fibronectin Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-05-14 Jackson K. B. Cahn, Henry Ludwicki, Jillian Shingler, Shannon Gulvin, Young Zhang, Adam Kristopeit, Christopher Ton, Michael A. Winters, James M. Wagner, Jeffrey Moore
Removal of host cell components is a significant cost driver in the production of live virus vaccines. Filtration processes such as tangential flow filtration can be effective in this capacity by leveraging the relative size difference between viral particles and host proteins; however, filtration membranes can be fouled by larger proteins, particularly those of the extracellular matrix. In this study
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Developing a Model for Virus Contamination in Perfusion Bioreactors to Establish Rational Control Strategies for Integrated Continuous Bioprocessing Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-05-14 Takao ITO, Takashi Nihei, Koichi Yamamoto, Naoto Watanabe, Yoshiyuki Tokieda, Yumiko Masuda, Masaki Okada
A viral contamination model for steady‐state perfusion cell culture was developed to assess how sampling frequency and volume impacted the expected downstream viral clearance factor in integrated continuous biomanufacturing processes. The model used population balance rate equations for cells and free virions, incorporating the virus infection cycle. It simulated the states of cell cultures for both
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Biotechnology and Bioengineering: Volume 122, Number 6, June 2025 Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-05-12
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Hybrid Modeling of the Reversed‐Phase Chromatographic Purification of an Oligonucleotide: Few‐Shot Learning From Differentiable Physics Solver‐in‐the‐Loop Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-05-09 Yu‐Cheng Chen, Ismaele Fioretti, Dong‐Qiang Lin, Mattia Sponchioni
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Protein Engineering for Enhancing Electron Transfer in P450‐Mediated Catalysis Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-05-09 Yuemin Li, Hongwei Yu, Lidan Ye
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RSEA: A Web Server for Pathway Enrichment Analysis of Metabolic Reaction Sets Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-05-09 Merve Yarıcı, Furkan Cantürk, Serdar Dursun, Hatice Nur Aydın, Muhammed Erkan Karabekmez
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A Genetic Algorithms‐Based Neural Network Model to Monitor Gibberellic Acid GA3 Fermentation Process by Fusarium fujikuroi Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-05-09 Jia‐Cong Huang, Jun‐Lin Wu, Zhi‐Kui Nie, Tian‐Qiong Shi
A genetic algorithm‐optimized neural network (ANN‐GA) was developed for real‐time monitoring of gibberellin (GA3) production during Fusarium fujikuroi fermentation. This model addresses the limitations of traditional off‐line detection methods, such as contamination risks and delayed feedback, by integrating six critical inputs—initial glucose concentration, fermentation time, temperature, pH, dissolved
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Cell‐Penetrating Peptide‐Based Triple Nanocomplex Enables Efficient Nuclear Gene Delivery in Chlamydomonas reinhardtii Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-05-08 Eun Jeong Sim, Quynh‐Giao Tran, Yu Rim Lee, Trang Thi Le, Hyang Ran Yoon, Dong‐Yun Choi, Dae‐Hyun Cho, Jin‐Ho Yun, Hong Il Choi, Hee‐Sik Kim, Yong Jae Lee
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A Comprehensive Model for Separating Systematic Bias and Noise in Metabolomic Timecourse Data—A Nonlinear B-Spline Mixed-Effects Approach Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-05-08 Kathy Sharon Isaac, Stanislav Sokolenko
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Isolation of Anammoxosomes From the Aggregate Culture of Ca. Brocadia Sapporoensis and Assembly of Ladderane Liposomes Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-05-08 Tomáš Podzimek, Terezie Cisarová, Michal Dvořák, Barbora Vokatá, Christina Karmann, Jaroslav Hanuš, Martin Balouch, Matěj Malý, Jana Hajšlová, Vojtěch Kouba, Jan Bartáček, František Štěpánek, Petra Lipovová
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Correction to “Sequential Assembly of Cell‐Laden Hydrogel Constructs to Engineer Vascular‐Like Microchannels” Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-05-06 Yanan Du, Majid Ghodousi, Hao Qi, Nikhil Haas, Wenqian Xiao, Ali Khademhosseini
In the published article, the images in Figure 4B,D were found to be from the same sample that was sequentially exposed to different conditions and analyzed. The authors have data from an independent experiment in which the gels were not sequentially analyzed (Figure 4 below). The updated figure does not alter the interpretation of the data or the conclusions of the study. We apologize for any inconvenience
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Detecting Excess Biofilm Thickness in Microbial Electrolysis Cells by Real‐Time In‐Situ Biofilm Monitoring Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-05-03 Andreas Netsch, Inka Latussek, Harald Horn, Michael Wagner
Long‐term stable operation of bioelectrochemical systems (BES) presupposes the avoidance of mass transfer limitations of the electroactive biofilm. Excessive pH‐gradients from bulk to electrode interface or substrate limitations of the electroactive biofilm are known to diminish the electrical performance of BES. In this study the impact of the morphology of a mixed‐species electroactive biofilm cultivated
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Mechanism‐Guided Protein Engineering of Paraburkholderia xenovorans Lactamase for the Green Synthesis of 2‐(2‐Oxopyrrolidin‐1‐yl)‐Butanoic Acid Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-05-03 Wenzhe Ma, Wanqing Wei, Jian Wen, Cong Gao, Guipeng Hu, Xiaomin Li, Jia Liu, Jing Wu, Wei Song
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Unlocking the Potential of Medicago truncatula A17 Cell Suspension Cultures for Bioproduction of Astaxanthin and Canthaxanthin Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-05-02 Susana M. Gomes, Bárbara A. Rebelo, Rita Abranches
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Switching Nitrilase With Enantioselective Hydration Activity for Efficient Synthesis of Chiral 2,2‐Dimethylcyclopropanecarboxamide Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-05-01 Xiao‐Ling Tang, Xu‐Gang Zhang, Ye‐Tao Zhang, Xian‐Ming Ye, Ren‐Chao Zheng, Yu‐Guo Zheng
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Process Intensification for Recombinant Protein Production in E. coli via Identification of Active Nodes in Cellular Metabolism and Dynamic Flux Balance Analysis Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-04-30 Hardik Dodia, Charandatta Muddana, Vivek Mishra, Avinash Vellore Sunder, Pramod P. Wangikar
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Engineering Precursor and Cofactor Metabolism in Escherichia Coli for Enhanced Adipic Acid Production From Glucose Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-04-30 Weijia Yuan, Chong Qiu, Jia Liu, Xiaomin Li, Guipeng Hu, Cong Gao, Liming Liu
The reverse adipate degradation pathway (RADP) for adipic acid synthesis has garnered significant interest. However, the limited efficiency of existing pathways and their dependence on plasmids have hindered the practical application of microbial cell factories. In this study, the efficiency of the adipic acid synthetic pathway was enhanced by substituting and combinatorially expressing RADP enzymes
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Scalable Cell‐Free Production of Active T7 RNA Polymerase Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-04-29 Ryan N. Rezvani, Rochelle Aw, Wei Chan, Krishnathreya Satish, Han Chen, Adi Lavy, Swechha Rimal, Divyesh A. Patel, Govind Rao, James R. Swartz, Matthew P. DeLisa, Erik Kvam, Ashty S. Karim, Antje Krüger, Weston Kightlinger, Michael C. Jewett
The SARS‐CoV‐2 pandemic highlighted the urgent need for biomanufacturing paradigms that are robust and fast. Here, we demonstrate the rapid process development and scalable cell‐free production of T7 RNA polymerase, a critical component in mRNA vaccine synthesis. We carry out a 1‐L cell‐free gene expression (CFE) reaction that achieves over 90% purity, low endotoxin levels, and enhanced activity relative
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Establishing a 3D Vascularized Tri‐Culture Model of the Human Airways via a Digital Light Processing Bioprinter Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-04-29 Sakshi Phogat, Tony Ju Feng Guo, Fama Thiam, Emmanuel Twumasi Osei
The rise in chronic lung diseases globally and the corresponding lag in drug discovery in this field highlights the need for In Vitro models closely mimicking In Vivo lung tissue. Efforts to date have largely focused on In Vitro coculture models, often neglecting the pulmonary vasculature's role in lung physiology and lacking perfusability. To address this gap, we utilized digital light processing
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3D‐Printed Scaffolds for Cranial Bone Regeneration: A Systematic Review of Design, Materials, and Computational Optimization Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-04-28 Elnaz Khorasani, Bahman Vahidi
Cranial bone defects from trauma, congenital conditions, or surgery are challenging to treat due to the skull's limited regeneration. Traditional methods like autografts and allografts have drawbacks, including donor site issues and poor integration. 3D‐printed scaffolds provide a patient‐specific alternative, improving bone regeneration and integration. This review evaluates advancements in 3D‐printed
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Microtiter Plate Cultivation Systems Enable Chemically Diverse Metabolic Footprints During Bacterial Natural Product Discovery Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-04-26 Anton Lindig, Georg Hubmann, Stephan Lütz
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A Mathematical Model for Determining Probabilistic Design Space in Mesenchymal Stem Cell Passage Culture Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-04-25 Keita Hirono, Yusuke Hayashi, Yuuki Ogawa, Masahiro Kino‐oka, Hirokazu Sugiyama
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Combinatorial Metabolic Engineering for Enhanced Gibberellic Acid Biosynthesis in Fusarium fujikuroi Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-04-24 Xia Ke, Yao Chen, Rui Jia, Hao-Nan Wang, Zhi-Qiang Liu, Yu-Guo Zheng
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Modeling Analysis of Oxygen Transfer Efficiency in Rest Cell Catalysis for Extra High-Titer Xylonic Acid Bioproduction Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-04-24 Xia Hua, Yong Xu
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Dynamic Remodeling of Mechano-Sensing Complexes in Suspended Fibroblast Cell-Sheets Under External Mechanical Stimulus Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-04-24 Madoka Suzuki, Keiko Kawauchi, Hiroaki Machiyama, Hiroaki Hirata, Shin'ichi Ishiwata, Hideaki Fujita
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Optimized Biosynthetic Pathway for Nonnatural Amino Acids: An Efficient Approach for L-2-Aminobutyric Acid Production Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-04-23 Jianmiao Xu, Yuan Tao, Qilan Shan, Yan Feng, Yihong Wang, Zhiqiang Liu, Yuguo Zheng
L-2-Aminobutyric acid (L-2-ABA) is a nonnatural chiral α-amino acid which is widely used in various chiral pharmaceuticals and medical intermediates. Currently, the microbial metabolic engineering approach to enable Escherichia coli to produce L-2-ABA autonomously exists the problem of low synthesis efficiency, limiting its large-scale application. In this study, we successfully constructed a strain
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Integrating Affinity Chromatography in the Platform Process for Adenovirus Purification Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-04-22 Yuxuan Wu, Eduardo Barbieri, William K. Smith, Arianna Minzoni, Ryan E. Kilgore, Wenning Chu, Michael A. Daniele, Stefano Menegatti
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Systems Metabolic Engineering of Clostridium tyrobutyricum for 1,3-Propanediol Production From Crude Glycerol Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-04-20 Yongzhang Mo, Xiaolong Guo, Yang Lan, Jufang Wang, Hongxin Fu
Clostridium tyrobutyricum has emerged as a non-pathogenic microbial cell factory capable of anaerobic production of various value-added products, such as butyrate, butanol, and butyl butyrate. This study reports the first systematic engineering of C. tyrobutyricum for the heterologous production of 1,3-propanediol (1,3-PDO) from industrial by-product crude glycerol. Initially, the glycerol reductive
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Correction to “Sequential Assembly of Cell-Laden Hydrogel Constructs to Engineer Vascular-Like Microchannels” Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-04-18
Y. Du, M. Ghodousi, H. Qi, N. Haas, W. Xiao, and A. Khademhosseini, “Sequential Assembly of Cell-Laden Hydrogel Constructs to Engineer Vascular-Like Microchannels,” Biotechnology and Bioengineering 108, no. 7 (2011): 1693–1703, 10.1002/bit.23102. This correction is published due to concerns raised by a third party regarding high similarity between the cell-laden microgels between Figure 4B,D. The authors
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Temporal Galactose-Manganese Feeding in Fed-Batch and Perfusion Bioreactors Modulates UDP-Galactose Pools for Enhanced mAb Glycosylation Homogeneity Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-04-18 Aron Gyorgypal, Erica Fratz-Berilla, Casey Kohnhorst, David N. Powers, Shishir P. S. Chundawat
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Heterologous Expression of Candida antarctica Lipase B in Aspergillus niger Using CRISPR/Cas9-mediated Multi-Gene Editing Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-04-18 Hongmei Nie, Mengmeng Jin, Zebin Wang, Jianyong Zheng, Yinjun Zhang, Xiaojun Li
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Altering 15-Lipoxygenases to 18-Lipoxygenases and Their Application to the Production of 5,18-Dihydroxyeicosapentaenoic Acids Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-04-16 Jin Lee, Su-Hwan Kang, Tae-Eui Lee, Deok-Kun Oh
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Modeling the Migration and Growth of Shewanella Oneidensis MR-1 in a Diffusion-Dominated Microfluidic Gradient Chamber Under the Influence of an Antibiotic Concentration Gradient Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-04-16 Somayeh G. Esfahani, Reinaldo E. Alcalde, Albert J. Valocchi, Robert A. Sanford, Charles J. Werth
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Erratum to “Dual-Stage Cross-Flow Filtration: Integrated Capture and Purification of Virus-Like Particles” Biotechnol. Bioeng. (IF 3.5) Pub Date : 2025-04-16
Dietrich, A., Heim, L. and Hubbuch, J. (2025), Dual-Stage Cross-Flow Filtration: Integrated Capture and Purification of Virus-Like Particles. Biotechnology and Bioengineering 122: 884–894. https://doi.org/10.1002/bit.28914 In the sections “Abstract”, “Introduction”, “Results”, “Discussion”, and “Conclusion”, as well as in the Graphical Abstract, Figure 1, the captions of Figures 1 and 2, and the supplementary