
样式: 排序: IF: - GO 导出 标记为已读
-
Standardizing cassette-based deep mutagenesis by Golden Gate assembly Biotechnol. Bioeng. (IF 3.8) Pub Date : 2023-09-26 Nicolas Daffern, Irene M. Francino-Urdaniz, Zachary T. Baumer, Timothy A. Whitehead
-
Construction of prophage-free and highly-transformable Limosilactobacillus reuteri strains and their use for production of 1,3-propanediol Biotechnol. Bioeng. (IF 3.8) Pub Date : 2023-09-25 Kalpana Singh, Sunghoon Park
The lactic acid bacterium Limosilactobacillus reuteri (formerly Lactobacillus reuteri) is a desirable host for the production of 1,3-propanediol (1,3-PDO) from glycerol when 1,3-PDO is used in the food or cosmetic industry. However, the production is hindered by strain instability, causing cell lysis, and difficult gene manipulation. This study reveals that the stability of L. reuteri DSM 20016 and
-
Multi-lipase gene knockdown in Chinese hamster ovary cells using artificial microRNAs to reduce host cell protein mediated polysorbate degradation Biotechnol. Bioeng. (IF 3.8) Pub Date : 2023-09-25 Linus Weiß, Valerie Schmieder-Todtenhaupt, Frank Haemmerling, Daniel Lakatos, Patrick Schulz, Simon Fischer
-
Identification of optimal flow rate for culture media, cell density, and oxygen toward maximization of virus production in a fed-batch baculovirus-insect cell system Biotechnol. Bioeng. (IF 3.8) Pub Date : 2023-09-25 Surbhi Sharma, Jagadeesh Mahadevan, Lopamudra Giri, Kishalay Mitra
-
Enhancing the production of recombinant adeno-associated virus in synthetic cell lines through systematic characterization Biotechnol. Bioeng. (IF 3.8) Pub Date : 2023-09-25 Min Lu, Zion Lee, Yu-Chieh Lin, Ibrahim Irfanullah, Wen Cai, Wei-Shou Hu
-
An extracellular glucose sensor for substrate-dependent secretion and display of cellulose-degrading enzymes Biotechnol. Bioeng. (IF 3.8) Pub Date : 2023-09-25 Pamela B. Besada-Lombana, Wilfred Chen, Nancy A. Da Silva
-
On-column capping of poly dT media-tethered mRNA accomplishes high capping efficiency, enhanced mRNA recovery, and improved stability against RNase Biotechnol. Bioeng. (IF 3.8) Pub Date : 2023-09-25 Shiyi Che, Xue Feng, Zhengjun Li, Zhiguo Su, Guanghui Ma, Zhikao Li, Aibing Yu, Minsu Liu, Songping Zhang
The messenger RNA (mRNA) 5′-cap structure is indispensable for mRNA translation initiation and stability. Despite its importance, large-scale production of capped mRNA through in vitro transcription (IVT) synthesis using vaccinia capping enzyme (VCE) is challenging, due to the requirement of tedious and multiple pre-and-post separation steps causing mRNA loss and degradation. Here in the present study
-
Research progress in the detection of common foodborne hazardous substances based on functional nucleic acids biosensors Biotechnol. Bioeng. (IF 3.8) Pub Date : 2023-09-18 Zijie Chen, Zhen Liu, Jingjing Liu, Xilin Xiao
-
Site-specific crosslinking and assembly of tetrameric β-glucuronidase improve glycyrrhizin hydrolysis Biotechnol. Bioeng. (IF 3.8) Pub Date : 2023-09-14 Qibin Wang, Yingying Wang, Xing Jian, Ning Wang, Chun Li, Hu Liu
-
CALU-3 lung cells three-dimensionally assembled onto CellFate® matrix present angiotensin-converting enzyme-2 activity Biotechnol. Bioeng. (IF 3.8) Pub Date : 2023-09-10 Jeniffer Farias dos Santos, Emily Marques dos Reis, Fernanda Vieira Berti, Guilherme Colla, Janice Koepp, Viviane Abreu Nunes
Currently, there is a great need for the development of three-dimensional (3D) in vitro lung models. Particularly, the production of a suitable 3D model of pulmonary epithelium for understanding the pathophysiology of diseases such as the COVID-19 must consider the tissue architecture and presence, for example, of the angiotensin-converting enzyme-2 (ACE-2) in the cells. Different polymeric membranes
-
Bioactive decellularized adipose matrix prepared using a rapid, nonchemical/enzymatic method for adipogenesis Biotechnol. Bioeng. (IF 3.8) Pub Date : 2023-09-10 Jizhong Yang, Jiezhang Tang, Juanli Dang, Xiangke Rong, Kai Wang, Zhaoxiang Zhang, Mengmeng Hou, Zhou Yu, Chenggang Yi
-
Recent advances in upstream process development for production of recombinant adeno-associated virus Biotechnol. Bioeng. (IF 3.8) Pub Date : 2023-09-10 Jianfa Ou, Yawen Tang, Jianlin Xu, Julian Tucci, Michael C. Borys, Anurag Khetan
-
Biocatalytic role of cytochrome P450s to produce antibiotics: A review Biotechnol. Bioeng. (IF 3.8) Pub Date : 2023-09-10 Anup Adhikari, Sajan Shakya, Shreesti Shrestha, Dipa Aryal, Kavi Prasad Timalsina, Dipesh Dhakal, Yogan Khatri, Niranjan Parajuli
-
Role of carbon nanotubes (CNTs) in transgenic plant development Biotechnol. Bioeng. (IF 3.8) Pub Date : 2023-09-10 Praniti Pawar, Sanjana Anumalla, Suvarna Sharma
-
Integrated continuous downstream process of monoclonal antibody developed by converting the batch platform process based on the process characterization Biotechnol. Bioeng. (IF 3.8) Pub Date : 2023-09-10 Fuminori Konoike, Masatoshi Taniguchi, Shuichi Yamamoto
A continuous downstream process of monoclonal antibody was developed based on the process characterization. Periodic-counter current chromatography (PCCC) with two protein A columns was used for the capture step. For low pH virus inactivation (VI), a batch reactor was employed, which can work as a surge (buffer) tank. Flow-through chromatography (FTC) with two connected columns of different separation
-
Characterization and designing of an SAM riboswitch to establish a high-throughput screening platform for SAM overproduction in Saccharomyces cerevisiae Biotechnol. Bioeng. (IF 3.8) Pub Date : 2023-09-10 Xiaomeng Fu, Xiaoru Zuo, Xiaomeng Zhao, Huizhi Zhang, Chuanbo Zhang, Wenyu Lu
-
Macrophage cell morphology-imprinted substrates can modulate mesenchymal stem cell behaviors and macrophage M1/M2 polarization for wound healing applications Biotechnol. Bioeng. (IF 3.8) Pub Date : 2023-09-05 Mohammad Majidi, Saeedreza Pakzad, Maryam Salimi, Abdolnaser Azadbakht, Saieh Hajighasemlou, Moein Amoupour, Zeinab Nokhbedehghan, Shahin Bonakdar, Koushan Sineh Sepehr, Narendra Pal Singh Chauhan, Mazaher Gholipourmalekabadi
-
The potential role of mechanotransduction in the management of pediatric calvarial bone flap repair Biotechnol. Bioeng. (IF 3.8) Pub Date : 2023-09-05 Hanna Anderson, David S. Hersh, Yusuf Khan
Pediatric patients suffering traumatic brain injuries may require a decompressive craniectomy to accommodate brain swelling by removing a portion of the skull. Once the brain swelling subsides, the preserved calvarial bone flap is ideally replaced as an autograft during a cranioplasty to restore protection of the brain, as it can reintegrate and grow with the patient during immature skeletal development
-
Combination of microbial and chemical synthesis for the sustainable production of β-elemene, a promising plant-extracted anticancer compound Biotechnol. Bioeng. (IF 3.8) Pub Date : 2023-09-04 Wenjuan Li, Jie Mai, Lu Lin, Zhi-Gang Zhang, Rodrigo Ledesma-Amaro, Weiliang Dong, Xiao-Jun Ji
-
Exploration and evaluation of modular concepts for the design of full-scale pharmaceutical manufacturing facilities Biotechnol. Bioeng. (IF 3.8) Pub Date : 2023-09-03 Maria-Ona Bertran, Deenesh K. Babi
-
An insight into biosensing platforms used for the diagnosis of various lung diseases: A review Biotechnol. Bioeng. (IF 3.8) Pub Date : 2023-09-03 Avinash Sharma, Abija James, Deepak N. Kapoor, Hemlata Kaurav, Abhishek Kumar Sharma, Rupak Nagraik
Many of the infectious diseases are ubiquitous in nature and pose a threat to global and public health. The original cause for such type of serious maladies can be summarized as the scarcity of appropriate analysis and treatment methods. Pulmonary diseases are considered one of the life-threatening lung diseases that affect millions of people globally. It consists of several types, namely, asthma,
-
Enhancement of the substrate specificity of D-amino acid oxidase based on tunnel-pocket engineering Biotechnol. Bioeng. (IF 3.8) Pub Date : 2023-08-31 Liuyu Wang, Heng Tang, Hongli Zhu, Yaping Xue, Yuguo Zheng
D-Amino acid oxidase (DAAO) selectively catalyzes the oxidative deamination of D-amino acids, making it one of the most promising routes for synthesizing optically pure L-amino acids, including L-phosphinothricin ( L-PPT), a chiral herbicide with significant market potential. However, the native DAAOs that have been reported have low activity against unnatural acid substrate D-PPT. Herein, we designed
-
Structural-guided design to improve the catalytic performance of aldo-keto reductase KdAKR Biotechnol. Bioeng. (IF 3.8) Pub Date : 2023-08-29 Chen Dai, Hai-Xing Cao, Jia-Xin Tian, Yan-Chi Gao, Hua-Tao Liu, Shen-Yuan Xu, Ya-Jun Wang, Yu-Guo Zheng
Aldo-keto reductases (AKRs) are important biocatalysts that can be used to synthesize chiral pharmaceutical alcohols. In this study, the catalytic activity and stereoselectivity of a NADPH-dependent AKR from Kluyveromyces dobzhanskii (KdAKR) toward t-butyl 6-chloro (5S)-hydroxy-3-oxohexanoate ((5S)-CHOH) were improved by mutating its residues in the loop regions around the substrate-binding pocket
-
CRISPR/Cas systems for in situ imaging of intracellular nucleic acids: Concepts and applications Biotechnol. Bioeng. (IF 3.8) Pub Date : 2023-08-28 Kun Chen, Yufei Wang
-
A new easy method for determination of surface adhesion of phototrophic biofilms Biotechnol. Bioeng. (IF 3.8) Pub Date : 2023-08-28 Judith Stiefelmaier, Dorina Strieth, Susanne Schaefer, Björn Wrabl, Daniel Kronenberger, Ulrich Bröckel, Roland Ulber
Terrestrial cyanobacteria grow as phototrophic biofilms and offer a wide spectrum of interesting products. For cultivation of phototrophic biofilms different reactor concepts have been developed in the last years. One of the main influencing factors is the surface material and the adhesion strength of the chosen production strain. In this work a flow chamber was developed, in which, in combination
-
Microtube embedded hydrogel bioprinting for vascularization of tissue-engineered scaffolds Biotechnol. Bioeng. (IF 3.8) Pub Date : 2023-08-28 Yan Chen, Liyuan Wang, Ying Wang, Yingge Zhou
Vascular tissue engineering has been considered promising as one of the alternatives for viable artificial tissues and organs. Macro- and microscale hollow tubes fabricated with various techniques have been widely studied to mimic blood vessels. To date, the fabrication of biomimetic capillary vessels with sizes ranging from 1 to 10 µm is still challenging. In this paper, core-sheath microtubes were
-
Eliminating genes for a two-component system increases PHB productivity in Cupriavidus basilensis 4G11 under PHB suppressing, nonstress conditions Biotechnol. Bioeng. (IF 3.8) Pub Date : 2023-08-28 Kyle Sander, Anthony J. Abel, Skyler Friedline, William Sharpless, Jeffrey Skerker, Adam Deutschbauer, Douglas S. Clark, Adam P. Arkin
Species of bacteria from the genus Cupriavidus are known, in part, for their ability to produce high amounts of poly-hydroxybutyrate (PHB) making them attractive candidates for bioplastic production. The native synthesis of PHB occurs during periods of metabolic stress, and the process regulating the initiation of PHB accumulation in these organisms is not fully understood. Screening an RB-TnSeq transposon
-
Recent advances in structure-based enzyme engineering for functional reconstruction Biotechnol. Bioeng. (IF 3.8) Pub Date : 2023-08-28 Shen-Yuan Xu, Lei Zhou, Ying Xu, Han-Yue Hong, Chen Dai, Ya-Jun Wang, Yu-Guo Zheng
-
Scale-up analysis of geometrically dissimilar single-use bioreactors Biotechnol. Bioeng. (IF 3.8) Pub Date : 2023-08-22 Diana Kreitmayer, Srikanth R. Gopireddy, Yuichi Aki, Koichi Nonaka, Nora A. Urbanetz, Eva Gutheil
Cell culture scale-up is a challenging task due to the simultaneous change of multiple hydrodynamic process characteristics and their different dependencies on the bioreactor size as well as variation in the requirements of individual cell lines. Conventionally, the volumetric power input is the most common parameter to select the impeller speed for scale-up, however, it is well reported that this
-
The application of electrosprayed minocycline-loaded PLGA microparticles for the treatment of glioblastoma Biotechnol. Bioeng. (IF 3.8) Pub Date : 2023-08-22 Marco A. Arriaga, Juan A. Amieva, Jaqueline Quintanilla, Angela Jimenez, Julio Ledezma, Silverio Lopez, Karen S. Martirosyan, Sue Anne Chew
The survival of patients with glioblastoma multiforme (GBM), the most common and invasive form of malignant brain tumors, remains poor despite advances in current treatment methods including surgery, radiotherapy, and chemotherapy. Minocycline is a semi-synthetic tetracycline derivative that has been widely used as an antibiotic and more recently, it has been utilized as an antiangiogenic factor to
-
Cashew nut shell oil as a potential feedstock for biodiesel production: An overview Biotechnol. Bioeng. (IF 3.8) Pub Date : 2023-08-22 Srinivasan Kandaswamy, Venkatesa Murthy Swarupa, Souvik Sur, Gautam Choubey, Yuvarajan Devarajan, Ruby Mishra
Biodiesel outperforms diesel in emissions and engine performance. They burn efficiently in diesel engines and are eco-friendly. Since cashew nut shell liquid (CNSO) is waste, commercial biodiesel production from it should be profitable. CNSO is cheap and can reduce cashew processing factory waste. From cashew kernels, CNSL is extracted using various mechanical, thermal, and solvent extraction techniques
-
High-performance countercurrent membrane purification for host cell protein removal from monoclonal antibody products Biotechnol. Bioeng. (IF 3.8) Pub Date : 2023-08-18 Aylin Mohammadzadehmarandi, Amy Determan, Christian Krumm, Lucas D. McIntosh, Andrew L. Zydney
-
Performance of cross-linked enzyme crystals of engineered halohydrin dehalogenase HheG in different chemical reactor systems Biotechnol. Bioeng. (IF 3.8) Pub Date : 2023-08-18 Marcel Staar, Anett Schallmey
-
Development of a novel purification method for AAV vectors using tangential flow filtration Biotechnol. Bioeng. (IF 3.8) Pub Date : 2023-08-16 Rimi Miyaoka, Yuji Tsunekawa, Yae Kurosawa, Takako Sasaki, Azusa Onodera, Kenji Sakamoto, Yuko Kakiuchi, Mikako Wada, Yuko Nitahara-Kasahara, Hiromi Hayashita-Kinoh, Takashi Okada
Adeno-associated virus (AAV) vector can efficiently transduce therapeutic genes in various tissue types with less side effects; however, owing to complex multistep processes during manufacture, there have been surges in the pricing of recently approved AAV vector-based gene therapy products. This study aimed to develop a simple and efficient method for high-quality purification of AAV vector via tangential
-
Characterization of a quail suspension cell line for production of a fusogenic oncolytic virus Biotechnol. Bioeng. (IF 3.8) Pub Date : 2023-08-16 Sven Göbel, Karim E. Jaén, Rita P. Fernandes, Manfred Reiter, Jennifer Altomonte, Udo Reichl, Yvonne Genzel
The development of efficient processes for the production of oncolytic viruses (OV) plays a crucial role regarding the clinical success of virotherapy. Although many different OV platforms are currently under investigation, manufacturing of such viruses still mainly relies on static adherent cell cultures, which bear many challenges, particularly for fusogenic OVs. Availability of GMP-compliant continuous
-
Cover Image, Volume 120, Number 9, September 2023 Biotechnol. Bioeng. (IF 3.8) Pub Date : 2023-08-15 Marta H. G. Costa, Margarida S. Costa, Beatriz Painho, Carolina D. Sousa, Inês Carrondo, Enrique Oltra, Beatriz Pelacho, Felipe Prosper, Inês A. Isidro, Paula Alves, Margarida Serra
-
Bioprocess development to produce a hyperthermostable S-methyl-5′-thioadenosine phosphorylas in Escherichia coli Biotechnol. Bioeng. (IF 3.8) Pub Date : 2023-08-13 Julia Schollmeyer, Saskia Waldburger, Kendra Njo, Heba Yehia, Anke Kurreck, Peter Neubauer, Sebastian L. Riedel
-
Continuous precipitation-filtration process for initial capture of a monoclonal antibody product using a four-stage countercurrent hollow fiber membrane washing step Biotechnol. Bioeng. (IF 3.8) Pub Date : 2023-08-10 Mirko Minervini, Matthew Mergy, Yuncan Zhu, Mario A. Gutierrez Diaz, Craig Pointer, Oleg Shinkazh, Sheldon F. Oppenheim, Steven M. Cramer, Todd M. Przybycien, Andrew L. Zydney
The significant increase in product titers, coupled with the growing focus on continuous bioprocessing, has renewed interest in using precipitation as a low-cost alternative to Protein A chromatography for the primary capture of monoclonal antibody (mAb) products. In this work, a commercially relevant mAb was purified from clarified cell culture fluid using a tubular flow precipitation reactor with
-
High performance anion exchange chromatography purification of probiotic bacterial extracellular vesicles enhances purity and anti-inflammatory efficacy Biotechnol. Bioeng. (IF 3.8) Pub Date : 2023-08-09 Nicholas H. Pirolli, Laura Samantha C. Reus, Zuzanna Mamczarz, Sulayman Khan, William E. Bentley, Steven M. Jay
Bacterial extracellular vesicles (BEVs), including outer membrane vesicles, have emerged as a promising new class of vaccines and therapeutics to treat cancer and inflammatory diseases, among other applications. However, clinical translation of BEVs is hindered by a current lack of scalable and efficient purification methods. Here, we address downstream BEV biomanufacturing limitations by developing
-
Thermostability enhancement of polyethylene terephthalate degrading PETase using self- and nonself-ligating protein scaffolding approaches Biotechnol. Bioeng. (IF 3.8) Pub Date : 2023-08-09 Barindra Sana, Ke Ding, Jia Wei Siau, Rupali Reddy Pasula, Sharon Chee, Sharad Kharel, Jean-Baptise Henri Lena, Eunice Goh, Lakshminarayanan Rajamani, Yeng Ming Lam, Sierin Lim, John F. Ghadessy
Polyethylene terephthalate (PET) hydrolase enzymes show promise for enzymatic PET degradation and green recycling of single-use PET vessels representing a major source of global pollution. Their full potential can be unlocked with enzyme engineering to render activities on recalcitrant PET substrates commensurate with cost-effective recycling at scale. Thermostability is a highly desirable property
-
Transcriptomics and cell painting analysis reveals molecular and morphological features associated with fed-batch production performance in CHO recombinant clones Biotechnol. Bioeng. (IF 3.8) Pub Date : 2023-08-09 Luke Nelson, Mike Veling, Fatemeh Farhangdoust, Xuezhu Cai, Steve Huhn, Veronica Soloveva, Meiping Chang
-
Evaluation of a single-use disk stack centrifuge for improved efficiency and sustainability at 1000 L GMP manufacturing scale Biotechnol. Bioeng. (IF 3.8) Pub Date : 2023-08-04 Joshua Walker, Yik Lam, Abdullah Loman, John P. Smelko, Michael Rohr
Direct depth filtration is an established technology for single-use harvest operation. Advantages of direct depth filtration include familiarity with depth filtration in downstream processes and simplicity of the operation. Drawbacks include low capacity, large footprint, labor-intensive set-up, high water use, and high waste in the form of discarded filters. Single-use centrifugation is emerging as
-
Dose–response analysis after administration of a human platelet-derived exosome product on neurite outgrowth in vitro Biotechnol. Bioeng. (IF 3.8) Pub Date : 2023-08-04 Sara Saffari, Daan J. Rademakers, Nicholas Pulos, Alexander Y. Shin
Modulating the nerve's local microenvironment using exosomes is proposed to enhance nerve regeneration. This study aimed to determine the optimal dose of purified exosome product (PEP) required to exert maximal neurite extension. An in vitro dorsal root ganglion (DRG) neurite outgrowth assay was used to evaluate the effect of treatment with (i) 5% PEP, (ii) 10% PEP, (iii) 15% PEP, or (iv) 20% PEP on
-
Automated calibration and in-line measurement of product quality during therapeutic monoclonal antibody purification using Raman spectroscopy Biotechnol. Bioeng. (IF 3.8) Pub Date : 2023-08-03 Jiarui Wang, Jingyi Chen, Joey Studts, Gang Wang
-
Intracellular accumulation of c-di-GMP and its regulation on self-flocculation of the bacterial cells of Zymomonas mobilis Biotechnol. Bioeng. (IF 3.8) Pub Date : 2023-08-01 Kai Li, Juan Xia, Chen-Guang Liu, Xin-Qing Zhao, Feng-Wu Bai
Zymomonas mobilis is an emerging chassis for being engineered to produce bulk products due to its unique glycolysis through the Entner–Doudoroff pathway with less ATP produced for lower biomass accumulation and higher product yield. When self-flocculated, the bacterial cells are more productive, since they can self-immobilize within bioreactors for high density, and are more tolerant to stresses for
-
Stable hydrogel adhesion to polydimethylsiloxane enables cyclic mechanical stimulation of 3D-bioprinted smooth muscle constructs Biotechnol. Bioeng. (IF 3.8) Pub Date : 2023-08-01 Zongzhe Xuan, Leonid Gurevich, Jesper de Claville Christiansen, Vladimir Zachar, Cristian Pablo Pennisi
-
Insights into product and process related challenges of lentiviral vector bioprocessing Biotechnol. Bioeng. (IF 3.8) Pub Date : 2023-08-01 Christopher Perry, Noor Mujahid, Yasu Takeuchi, Andrea C. M. E. Rayat
Lentiviral vectors (LVs) are used in advanced therapies to transduce recipient cells for long term gene expression for therapeutic benefit. The vector is commonly pseudotyped with alternative viral envelope proteins to improve tropism and is selected for enhanced functional titers. However, their impact on manufacturing and the success of individual bioprocessing unit operations is seldom demonstrated
-
Using enhanced development tools offered by analytical Quality by Design to support switching of a quality control method Biotechnol. Bioeng. (IF 3.8) Pub Date : 2023-08-01 Philippe Simeoni, Michael Deissler, Roland Bienert, Manuela Gritsch, Jörg Nerkamp, Stephan Kirsch, Christoph Roesli, Thomas Pohl, Oliver Anderka, Gerald Gellermann
Quality by Design (QbD) principles play an increasingly important role in the pharmaceutical industry. Here, we used an analytical QbD (AQbD) approach to develop a capillary electrophoresis sodium dodecyl sulfate under reducing conditions (rCE-SDS), with the aim of replacing SDS-polyacrylamide gel electrophoresis (SDS-PAGE) as release and stability test method for a commercialized monoclonal antibody
-
Continuous polyhydroxybutyrate production from biogas in an innovative two-stage bioreactor configuration Biotechnol. Bioeng. (IF 3.8) Pub Date : 2023-07-27 Yadira Rodríguez, Silvia García, Raquel Lebrero, Raúl Muñoz
-
Nonlinear state estimation as tool for online monitoring and adaptive feed in high throughput cultivations Biotechnol. Bioeng. (IF 3.8) Pub Date : 2023-07-27 Annina Kemmer, Nico Fischer, Terrance Wilms, Linda Cai, Sebastian Groß, Rudibert King, Peter Neubauer, M. Nicolas Cruz Bournazou
Robotic facilities that can perform advanced cultivations (e.g., fed-batch or continuous) in high throughput have drastically increased the speed and reliability of the bioprocess development pipeline. Still, developing reliable analytical technologies, that can cope with the throughput of the cultivation system, has proven to be very challenging. On the one hand, the analytical accuracy suffers from
-
Protein fouling during constant-flux virus filtration: Mechanisms and modeling Biotechnol. Bioeng. (IF 3.8) Pub Date : 2023-07-25 Joshua Peles, Benjamin Cacace, Christina Carbrello, Sal Giglia, Andrew L. Zydney
-
Improving phytase production in Pichia pastoris fermentations through de-repression and methanol induction optimization Biotechnol. Bioeng. (IF 3.8) Pub Date : 2023-07-25 Carlos H. Luna-Flores, Yilun Weng, Alexander Wang, Xiaojing Chen, Bingyin Peng, Chun-Xia Zhao, Laura Navone, Juhani von Hellens, Robert E. Speight
-
Sodium chloride impacts glycosylation and N- and O-glycan site occupancy of an Fc-fusion protein Biotechnol. Bioeng. (IF 3.8) Pub Date : 2023-07-25 Susanne Bohl, Maxime Le Mignon, Thomas Kilian, Aline Zimmer
-
Implications of AAV affinity column reuse and vector stability on product quality attributes Biotechnol. Bioeng. (IF 3.8) Pub Date : 2023-07-24 Harshal Soni, Ira Lako, Matteo Placidi, Steven M. Cramer
In this work, the implications of AAV9 capsid design and column reuse on AAV9 vector product quality were assessed with POROS CaptureSelect (PCS) AAVX and AAV9 resins using sf9 insect cell-derived model AAV9 vectors with varying viral protein (VP) ratios. Chromatographic experiments with purified drug substance AAV9 model feeds indicated consistent vector elution profiles, independent of adeno-associated
-
Design space determination to optimize DNA complexation and full capsid formation in transient rAAV manufacturing Biotechnol. Bioeng. (IF 3.8) Pub Date : 2023-07-21 Qiang Fu, Yong Suk Lee, Erica A. Green, Yongdan Wang, So Young Park, Ashli Polanco, Kelvin H. Lee, Michael Betenbaugh, David McNally, Seongkyu Yoon
Recombinant adeno-associated virus (rAAV) vectors are a promising platform for in vivo gene therapies. However, cost-effective, well-characterized processes necessary to manufacture rAAV therapeutics are challenging to develop without an understanding of how process parameters (PPs) affect rAAV product quality attributes (PQAs). In this work, a central composite orthogonal experimental design was employed
-
Synchrotron radiation-based microcomputed tomography for three-dimensional growth analysis of Aspergillus niger pellets Biotechnol. Bioeng. (IF 3.8) Pub Date : 2023-07-21 Henri Müller, Charlotte Deffur, Stefan Schmideder, Lars Barthel, Tiaan Friedrich, Lukas Mirlach, Jörg U. Hammel, Vera Meyer, Heiko Briesen
-
Sensors and chemometrics in downstream processing Biotechnol. Bioeng. (IF 3.8) Pub Date : 2023-07-20 Astrid Dürauer, Alois Jungbauer, Theresa Scharl
-
Exploration of computational approaches to predict the structural features and recent trends in α-amylase production for industrial applications Biotechnol. Bioeng. (IF 3.8) Pub Date : 2023-07-21 Mohsin Shad, Naveed Hussain, Muhammad Usman, Muhammad W. Akhtar, Muhammad Sajjad
Amylases are biologically active enzymes that can hydrolyze starch to produce dextrin, glucose, maltose, and oligosaccharides. The amylases contribute approximately 30% to the global industrial enzyme market. The globally produced amylases are widely used in textile, biofuel, starch processing, food, bioremediation of environmental pollutants, pulp, and paper, clinical, and fermentation industries
-
Concentration-dependent diffusion of unlabeled protein within an in vitro hyaluronic acid matrix Biotechnol. Bioeng. (IF 3.8) Pub Date : 2023-07-19 Antonio C. F. dos Santos, Adib Ahmadzadegan, Eduardo Ximenes, Pavlos Vlachos, Arezoo Ardekani, Shiven Kapur, Vince Corvari, Michael R. Ladisch
-
Methodology for fast development of digital solutions in integrated continuous downstream processing Biotechnol. Bioeng. (IF 3.8) Pub Date : 2023-07-17 Niklas Andersson, Joaquín Gomis Fons, Madelène Isaksson, Simon Tallvod, Daniel Espinoza, Linnea Sjökvist, Gusten Zandler Andersson, Bernt Nilsson
The methodology for production of biologics is going through a paradigm shift from batch-wise operation to continuous production. Lot of efforts are focused on integration, intensification, and continuous operation for decreased foot-print, material, equipment, and increased productivity and product quality. These integrated continuous processes with on-line analytics become complex processes, which