Recent advances in cell-free protein synthesis have enabled the folding and assembly of full-length antibodies at high titers with extracts from prokaryotic cells. Coupled with the facile engineering of the E. coli translation machinery, E. coli based in vitro protein synthesis reactions have emerged as a leading source of IgG molecules with non-natural amino acids incorporated at specific locations

The N-glycan pattern of an IgG antibody, attached at a conserved site within the Fc region, is a critical antibody quality attribute whose structural variability can also impact antibody function. For tailoring the Fc glycoprofile, glycoengineering in cell lines as well as Fc amino acid mutations have been applied. Multiple glycoengineered CHO cell lines were generated, including defucosylated (FUT8KO)

This erratum is being published to correct the mistake in Figure 3 caption, we wrote Figures 3a and 3b for different magnifications for H&E staining images. The Figure 3 caption “Figure 3. Histological and immunohistochemical staining of the cell-seeded hydrogels. (a and b) Sections were stained with H&E at (a) day 14 and (b) day 28; (c and d) sections were stained with Safranin O for GAGs after (c)

Microbial superhost strains should provide an ideal platform for the efficient homologous or heterologous phenotypic expression of biosynthetic gene clusters (BGCs) of new and novel bioactive molecules. Our aim in the current study was to perform a comparative study at the bioprocess and metabolite levels of the previously designed superhost strain Streptomyces coelicolor M1152 and its derivative strain

Adventitious agent testing in biomanufacturing requires assays of broad detection capability in order to screen for as many infectious agents as possible. The current gold standard for general infectious adventitious virus screening is the in vitro assay in which test articles are cultured onto a panel of different cell lines and observed for cytopathic effect (CPE). However, this assay is inherently

Effects of fluid dynamics on cells are often studied by growing the cells on the base of cylindrical wells or dishes that are swirled on the horizontal platform of an orbital shaker. The swirling culture medium applies a shear stress to the cells that varies in magnitude and directionality from the centre to the edge of the vessel. Computational fluid dynamics methods are used to simulate the flow

Manufacturing has been the key factor limiting rollout of vaccination during the COVID-19 pandemic, requiring rapid development and large-scale implementation of novel manufacturing technologies.