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End to End Collaboration to Transform Biopharmaceutical Development and Manufacturing Biotechnol. Bioeng. (IF 4.002) Pub Date : 2021-01-22 John Erickson; Jeffrey Baker; Shawn Barrett; Ciaran Brady; Mark Brower; Ruben Carbonell; Tim Charlebois; Jon Coffman; Lisa Connell‐Crowley; Michael Coolbaugh; Eric Fallon; Eric Garr; Christopher Gillespie; Roger Hart; Allison Haug; Gregg Nyberg; Michael Phillips; David Pollard; Maen Qadan; Irina Ramos; Kelley Rogers; Gene Schaefer; Jason Walther; Kelvin Lee
An ambitious ten‐year collaborative program is described to invent, design, demonstrate and support commercialization of integrated biopharmaceutical manufacturing technology intended to transform the industry. Our goal is to enable improved control, robustness and security of supply, and dramatically reduced capital and operating cost, flexibility to supply an extremely diverse and changing portfolio
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Non‐Viral Gene Delivery to T Cells with Lipofectamine LTX Biotechnol. Bioeng. (IF 4.002) Pub Date : 2021-01-22 Emily Harris; Devon Zimmerman; Eric Warga; Anil Bamezai; Jacob Elmer
Retroviral gene delivery is widely used in T cell therapies for hematological cancers. However, viral vectors are expensive to manufacture, integrate genes in semi‐random patterns, and their transduction efficiency varies between patients. In this study, several non‐viral gene delivery vehicles, promoters, and additional variables were compared to optimize non‐viral transgene delivery and expression
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Process development and optimization of continuous capture with three‐column periodic counter‐current chromatography Biotechnol. Bioeng. (IF 4.002) Pub Date : 2021-01-22 Ce Shi; Qi‐Lei Zhang; Biao Jiao; Xu‐Jun Chen; Ran Chen; Wei Gong; Shan‐Jing Yao; Dong‐Qiang Lin
Continuous capture with affinity chromatography is one of the most important units for continuous manufacturing of monoclonal antibody (mAb). Due to the complexity of three‐column periodic counter‐current chromatography (3C‐PCC), three approaches (experimental, model‐based and simplified approaches) were studied for process development and optimization. The effects of residence time for interconnected
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Spatial distribution of mechanical properties in Pseudomonas aeruginosa biofilms, and their potential impacts on biofilm deformation Biotechnol. Bioeng. (IF 4.002) Pub Date : 2021-01-07 Juan P. Pavissich; Mengfei Li; Robert Nerenberg
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Miniaturized electrochemical biosensor based on whole‐cell for heavy metal ions detection in water Biotechnol. Bioeng. (IF 4.002) Pub Date : 2020-12-07 Emanuele L. Sciuto; Salvatore Petralia; Jan R. van der Meer; Sabrina Conoci
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Incorporating RNA‐Seq transcriptomics into glycosylation‐integrating metabolic network modelling kinetics: Multiomic Chinese hamster ovary (CHO) cell bioreactors Biotechnol. Bioeng. (IF 4.002) Pub Date : 2021-01-05 Lara Bezjak; Vivian Erklavec Zajec; Špela Baebler; Tjaša Stare; Kristina Gruden; Andrej Pohar; Uroš Novak; Blaž Likozar
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A high‐throughput and machine learning resistance monitoring system to determine the point of resistance for Escherichia coli with tetracycline: Combining UV‐visible spectrophotometry with principal component analysis Biotechnol. Bioeng. (IF 4.002) Pub Date : 2021-01-05 James Chapman; Rebecca Orrell‐Trigg; Ki Y. Kwoon; Vi K. Truong; Daniel Cozzolino
UV‐visible spectroscopy (UV‐Vis) is routinely used in microbiology as a tool to check the optical density (OD) pertaining to the growth stages of microbial cultures at the single wavelength of 600 nm, better known as the OD600. Typically, modern UV‐Vis spectrophotometers can scan in the region of approximately 200–1000 nm in the electromagnetic spectrum, where users do not extend the use of the instrument's
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Continued insights into virus clearance validation across continuous capture chromatography Biotechnol. Bioeng. (IF 4.002) Pub Date : 2021-01-09 James M. Angelo; Kevin Potter; Thomas Müller‐Späth; Xuankuo Xu; Zheng Jian Li; Sanchayita Ghose
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Substrate availability drives mixed culture fermentation of glucose to lactate at steady state Biotechnol. Bioeng. (IF 4.002) Pub Date : 2021-01-12 Robert D. Hoelzle; Daniel Puyol; Bernardino Virdis; Damien Batstone
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Biochemical characterization of a novel thermo‐halo‐tolerant GH5 endoglucanase from a thermal spring metagenome Biotechnol. Bioeng. (IF 4.002) Pub Date : 2021-01-06 Namrata Joshi; Girija Kaushal; Sudhir P. Singh
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Uncoupling growth and succinic acid production in an industrial Saccharomyces cerevisiae strain Biotechnol. Bioeng. (IF 4.002) Pub Date : 2021-01-06 Yaya Liu; Osman Esen; Jack T. Pronk; Walter M. van Gulik
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Biomanufacturing of Value‐Added Products from Oils or Fats: A Case Study on Cellular and Fermentation Engineering of Yarrowia lipolytica Biotechnol. Bioeng. (IF 4.002) Pub Date : 2021-01-20 Na Liu; Ya‐Hue Valerie Soong; Iman Mirzaee; Andrew Olsen; Peng Yu; Hsi‐Wu Wong; Dongming Xie
The United States produces more than 10 million tons of waste oils and fats each year. This paper aims to establish a new biomanufacturing platform that convert waste oils or fats into a series of value‐added products. Our research employs the oleaginous yeast Yarrowia lipolytica as a case study for citric acid (CA) production from waste oils. First, we conducted the CFD simulation of the bioreactor
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Metabolic engineering of HEK293 cells to improve transient transfection and cell budding of HIV‐1 virus‐like particles Biotechnol. Bioeng. (IF 4.002) Pub Date : 2021-01-19 Jesús Lavado‐García; Andy Díaz‐Maneh; Núria Canal‐Paulí; Pol Pérez‐Rubio; Francesc Gòdia; Laura Cervera
HIV‐1 Gag virus‐like particles (VLPs) are promising candidates for the development of future vaccines. Recent viral outbreaks have manifested the need of robust vaccine production platforms able to adapt to new challenges while achieving mass production capacity. For the rapid production of VLPs, the method of transient gene expression (TGE) have proved highly efficient. Based on a previous characterization
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Magnetoelastic Sensors for Real‐Time Tracking of Cell Growth Biotechnol. Bioeng. (IF 4.002) Pub Date : 2021-01-18 Sudhanshu Shekhar; Salil S. Karipott; Robert E. Guldberg; Keat Ghee Ong
Magnetoelastic (ME) sensors, which can be remotely activated via magnetic fields, are an excellent choice for wireless monitoring of biological parameters due to their ability to be scaled into different sizes and have their surface functionalized for chemical or biological sensing. In this study, we present the application of a commercially available ME material (Metglas 2826 MB) to develop a sensor
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An integrated continuous downstream process with real‐time control: A case study with periodic countercurrent chromatography and continuous virus inactivation Biotechnol. Bioeng. (IF 4.002) Pub Date : 2021-01-18 Anton Löfgren; Joaquin Gomis‐Fons; Niklas Andersson; Bernt Nilsson; Lotta Berghard; Christine Lagerquist Hägglund
Integrated continuous downstream processes with process analytical technology offer a promising opportunity to reduce production costs and increase process flexibility and adaptability. In this case study, an integrated continuous process was used to purify a recombinant protein on laboratory scale in a two‐system setup that can be used as a general downstream setup offering multi‐product and multi‐purpose
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Balancing glucose and oxygen uptake rates to enable high amorpha‐4,11‐diene production in Escherichia coli via the methylerythritol phosphate pathway Biotechnol. Bioeng. (IF 4.002) Pub Date : 2020-12-17 Vikas Patil; Christine N. S. Santos; Parayil K. Ajikumar; Stephen Sarria; Ralf Takors
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Temperature regulation as a tool to program synthetic microbial community composition Biotechnol. Bioeng. (IF 4.002) Pub Date : 2021-01-05 Adam G. Krieger; Jiahao Zhang; Xiaoxia N. Lin
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Flux redistribution of central carbon metabolism for efficient production of l‐tryptophan in Escherichia coli Biotechnol. Bioeng. (IF 4.002) Pub Date : 2021-01-05 Bo Xiong; Yongduo Zhu; Daoguang Tian; Shuai Jiang; Xiaoguang Fan; Qian Ma; Heyun Wu; Xixian Xie
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Real‐time ensemble microalgae growth forecasting with data assimilation Biotechnol. Bioeng. (IF 4.002) Pub Date : 2021-01-05 Hongxiang Yan; Mark S. Wigmosta; Ning Sun; Michael H. Huesemann; Song Gao
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H2 production by photofermentation in an innovative plate‐type photobioreactor with meandering channels Biotechnol. Bioeng. (IF 4.002) Pub Date : 2020-12-15 Violette Turon; Stéphane Ollivier; Gregory Cwicklinski; John C. Willison; Zoé Anxionnaz‐Minvielle
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Development of an in vitro coculture device for the investigation of host‐microbe interactions via integrative multiomics approaches Biotechnol. Bioeng. (IF 4.002) Pub Date : 2021-01-09 Won‐Suk Song; Sung Gyu Shin; Sung‐Hyun Jo; Jae‐Seung Lee; Hyo‐Jin Jeon; Ji‐Eun Kwon; Ji‐Hyeon Park; Sungwoo Cho; Jae Hyun Jeong; Byung‐Gee Kim; Yun‐Gon Kim
The commensal gut bacterium Akkermansia muciniphila is well known as a promising probiotic candidate that improves host health and prevents diseases. However, the biological interaction of A. muciniphila with human gut epithelial cells has rarely been explored for use in biotherapeutics. Here, we developed an in vitro device that simulates the gut epithelium to elucidate the biological effects of living
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Biologically rapid synthesized silver nanoparticles from aqueous Eucalyptus camaldulensis leaf extract: effects on hyphal growth, hydrolytic enzymes, and biofilm formation in Candida albicans Biotechnol. Bioeng. (IF 4.002) Pub Date : 2021-01-09 Suttiwan Wunnoo; Supakit Paosen; Sakkarin Lethongkam; Rattanavadee Sukkurd; Tassanai Waen‐ngoen; Taiyeebah Nuidate; Manthana Phengmak; Supayang P. Voravuthikunchai
Bionanotechnology has increasingly gained attention in biomedical fields as antifungal and antibiofilm agents. This study biosynthesized silver nanoparticles (bio‐AgNPs) using aqueous Eucalyptus camaldulensis leaf extract were successfully performed by a one‐step green approach. Spherical‐shaped nanoparticles, approximate size of 8.65 nm exhibited non‐cytotoxicity to erythrocytes, HeLa, and HaCaT cells
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Pilot‐scale demonstration of an end‐to‐end integrated and continuous biomanufacturing process Biotechnol. Bioeng. (IF 4.002) Pub Date : 2021-01-07 Michael J. Coolbaugh; Chad T. Varner; Tarl A. Vetter; Emily K. Davenport; Brad Bouchard; Marcus Fiadeiro; Nihal Tugcu; Jason Walther; Rohan Patil; Kevin Brower
There has been increasing momentum recently in the biopharmaceutical industry to transition from traditional batch processes to next‐generation integrated and continuous biomanufacturing. This transition from batch to continuous is expected to offer several advantages which, taken together, could significantly improve access to biologics drugs for patients. Despite this recent momentum, there has not
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A CRISPR‐Cas12a‐Derived Biosensor Enabling Portable Personal Glucose Meter Readout for Quantitative Detection of SARS‐CoV‐2 Biotechnol. Bioeng. (IF 4.002) Pub Date : 2021-01-06 Di Huang; Zhuwei Shi; Jiajie Qian; Ke Bi; Mengjun Fang; Zhinan Xu
SARS‐CoV‐2 has spread rapidly throughout the whole world and caused significant difficulties in the prevention and control of the epidemic. In this case, several detection methods have been established based on nucleic acid diagnostic techniques and immunoassays to achieve sensitive and specific detection of SARS‐CoV‐2. However, most methods are still largely dependent on professional instruments,
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Microfluidics for Microalgal Biotechnology Biotechnol. Bioeng. (IF 4.002) Pub Date : 2021-01-06 Berin Ozdalgic; Merve Ustun; Sajjad Rahmani Dabbagh; Berat Z. Haznedaroglu; Alper Kiraz; Savas Tasoglu
Microalgae have expanded their roles as renewable and sustainable feedstocks for biofuel, smart nutrition, biopharmaceutical, cosmeceutical, biosensing, and space technologies. They accumulate valuable biochemical compounds from protein, carbohydrate, and lipid groups, including pigments and carotenoids. Microalgal biomass, which can be adopted for multivalorization under biorefinery settings, allows
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Multi‐omics analysis of hiPSCs‐derived HLCs matured on‐chip revealed patterns typical of liver regeneration Biotechnol. Bioeng. (IF 4.002) Pub Date : 2021-01-06 Mathieu Danoy; Yannick Tauran; Stéphane Poulain; Rachid Jellali; Johanna Bruce; Marjorie Leduc; Morgane Le Gall; Francoise Gilard; Taketomo Kido; Hiroshi Arakawa; Karin Araya; Daiki Mori; Yukio Kato; Hiroyuki Kusuhara; Charles Plessy; Atsushi Miyajima; Yasuyuki Sakai; Eric Leclerc
Maturation of human‐induced pluripotent stem cells (hiPSCs)‐derived hepatocytes‐like cells (HLCs) toward a complete hepatocyte phenotype remains a challenge as primitiveness patterns are still commonly observed. In this work, we propose a modified differentiation protocol for those cells which includes a pre‐maturation in Petri dishes and a maturation in microfluidic biochip. For the first time, a
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Creation and Filtering of a Recurrent Spectral Library of CHO Cell Metabolites and Media Components Biotechnol. Bioeng. (IF 4.002) Pub Date : 2021-01-06 Kelly H. Telu; Ramesh Marupaka; Nirina R. Andriamaharavo; Yamil Simón‐Manso; Yuxue Liang; Yuri A. Mirokhin; Tallat H. Bukhari; Renae J. Preston; Lila Kashi; Zvi Kelman; Stephen E. Stein
This paper reports the first implementation of a new type of mass spectral library for the analysis of Chinese hamster ovary (CHO) cell metabolites that allows users to quickly identify most compounds in any complex metabolite sample. We also describe an annotation methodology developed to filter out artifacts and low‐quality spectra from recurrent unidentified spectra of metabolites. CHO cells are
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A peptide‐based coating toolbox to enable click chemistry on polymers, metals, and silicon through sortagging Biotechnol. Bioeng. (IF 4.002) Pub Date : 2021-01-06 Maximilian Nöth; Zhi Zou; Islam El‐Awaad; Letícia Celia de Lencastre Novaes; Guilherme Dilarri; Mehdi D. Davari; Henrique Ferreira; Felix Jakob; Ulrich Schwaneberg
A versatile peptide‐toolbox for surface functionalization was established by a combination of a universal material binding peptide (LCI‐anchor peptide) and sortase‐mediated bioconjugation (sortagging). This toolbox facilitates surface functionalization either as a one‐ or a two‐step strategy. In case of the one‐step strategy, the desired functionality was directly introduced to LCI. For the two‐step
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Linear flow‐velocity gradient chromatography—An efficient method for increasing the process efficiency of batch and continuous capture chromatography of proteins Biotechnol. Bioeng. (IF 4.002) Pub Date : 2020-12-07 Chyi‐Shin Chen; Kosei Ando; Noriko Yoshimoto; Shuichi Yamamoto
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Production and monitoring of biomass and fucoxanthin with brown microalgae under outdoor conditions Biotechnol. Bioeng. (IF 4.002) Pub Date : 2020-12-15 Fengzheng Gao; Marta Sá; Iago Teles (Cabanelas, ITD); René H. Wijffels; Maria J. Barbosa
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Temperature and oxygen level determine N2O respiration activities of heterotrophic N2O‐reducing bacteria: Biokinetic study Biotechnol. Bioeng. (IF 4.002) Pub Date : 2020-12-11 Yiwen Zhou; Toshikazu Suenaga; Chuang Qi; Shohei Riya; Masaaki Hosomi; Akihiko Terada
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Introduction of glycine synthase enables uptake of exogenous formate and strongly impacts the metabolism in Clostridium pasteurianum Biotechnol. Bioeng. (IF 4.002) Pub Date : 2020-12-17 Yaeseong Hong; Philipp Arbter; Wei Wang; Lilian N. Rojas; An‐Ping Zeng
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How to measure diffusion coefficients in biofilms: A critical analysis Biotechnol. Bioeng. (IF 4.002) Pub Date : 2020-12-07 Lenno van den Berg; Mark C. M. van Loosdrecht; Merle K. de Kreuk
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Long‐term biofouling formation mediated by extracellular proteins in Nannochloropsis gaditana microalga cultures at different medium N/P ratios Biotechnol. Bioeng. (IF 4.002) Pub Date : 2020-11-25 Y. Soriano‐Jerez; L. López‐Rosales; M. C. Cerón‐García; A. Sánchez‐Mirón; J. J. Gallardo‐Rodríguez; F. García‐Camacho; E. Molina‐Grima
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Mineralized paper scaffolds for bone tissue engineering Biotechnol. Bioeng. (IF 4.002) Pub Date : 2020-12-11 Xinchen Wu; Kierra Walsh; Sanika Suvarnapathaki; Darlin Lantigua; Colleen McCarthy; Gulden Camci‐Unal
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In situ tracking of microbeads for the detection of biofilm formation Biotechnol. Bioeng. (IF 4.002) Pub Date : 2020-12-10 Héloïse Boudarel; Jean‐Denis Mathias; Benoît Blaysat; Michel Grédiac
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Responsive polymer‐assisted 3D cryogel supports Huh7.5 as in vitro hepatitis C virus model and ectopic human hepatic tissue in athymic mice Biotechnol. Bioeng. (IF 4.002) Pub Date : 2020-12-09 Priyanka Jayal; Padmanava Behera; Ranajoy Mullick; Subbaraya G. Ramachandra; Saumitra Das; Ashok Kumar; Anjali Karande
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Effect of alginate matrix engineered to mimic the pancreatic microenvironment on encapsulated islet function Biotechnol. Bioeng. (IF 4.002) Pub Date : 2020-12-03 Kevin Enck; Riccardo Tamburrini; Chaimov Deborah; Carlo Gazia; Alec Jost; Fatma Khalil; Abdelrahman Alwan; Giuseppe Orlando; Emmanuel C. Opara
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Biofilm structure, dynamics, and ecology of an upscaled biocathode wastewater microbial fuel cell Biotechnol. Bioeng. (IF 4.002) Pub Date : 2020-12-11 Aaron Leininger; Matthew D. Yates; Mark Ramirez; Birthe Kjellerup
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Macroscopic modeling of bioreactors for recombinant protein producing Pichia pastoris in defined medium Biotechnol. Bioeng. (IF 4.002) Pub Date : 2020-12-04 Moo Sun Hong; M. Lourdes Velez‐Suberbie; Andrew J. Maloney; Andrew Biedermann; Kerry R. Love; J. Christopher Love; Tarit K. Mukhopadhyay; Richard D. Braatz
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Indirect regulation of temperature in raceway reactors by optimal management of culture depth Biotechnol. Bioeng. (IF 4.002) Pub Date : 2020-12-03 E. Rodríguez‐Miranda; J. L. Guzmán; F. G. Acién; M. Berenguel; A. Visioli
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PAMAM G4.5 dendrimers for targeted delivery of ferulic acid and paclitaxel to overcome P‐glycoprotein‐mediated multidrug resistance Biotechnol. Bioeng. (IF 4.002) Pub Date : 2020-12-07 Rajeshkumar Anbazhagan; Ganesan Muthusamy; Rajakumari Krishnamoorthi; Swedha Kumaresan; Nagarajan Rajendra Prasad; Juin‐Yih Lai; Jen‐Ming Yang; Hsieh‐Chih Tsai
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Engineering Soluble Artificial Epidermal Growth Factor Receptor Mimics Capable of Spontaneous in vitro Dimerization Biotechnol. Bioeng. (IF 4.002) Pub Date : 2020-12-17 Allison Sunderhaus; Ramsha Imran; Amanda Goudelock; Manon Nassar; Kendall Cooper; Dustin Patterson; May H. Abdel Aziz
Epidermal Growth Factor Receptor (EGFR) is a clinically validated target for a multitude of human cancers. The receptor is activated upon ligand binding through a critical dimerization step. Dimerization can be replicated in vitro by locally concentrating the receptor kinase domains on the surface of lipid‐based vesicles. In this work we investigated the use of coiled coils to induce spontaneous receptor
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Reduced type‐A carbohydrate‐binding module interactions to cellulose I leads to improved endocellulase activity Biotechnol. Bioeng. (IF 4.002) Pub Date : 2020-11-27 Bhargava Nemmaru; Nicholas Ramirez; Cindy J. Farino; John M. Yarbrough; Nicholas Kravchenko; Shishir P. S. Chundawat
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High‐ and low‐molecular‐weight chitosan act as adjuvants during single‐dose influenza A virus protein vaccination through distinct mechanisms Biotechnol. Bioeng. (IF 4.002) Pub Date : 2020-12-07 Anna T. Lampe; Eric J. Farris; Deborah M. Brown; Angela K. Pannier
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Non‐xenogeneic expansion and definitive endoderm differentiation of human pluripotent stem cells in an automated bioreactor Biotechnol. Bioeng. (IF 4.002) Pub Date : 2020-11-18 Elena F. Jacobson; Zijing Chen; Demetrios M. Stoukides; Gopika G. Nair; Matthias Hebrok; Emmanuel S. Tzanakakis
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Halophilic archaea and their potential to generate renewable fuels and chemicals Biotechnol. Bioeng. (IF 4.002) Pub Date : 2020-11-26 Lakshmi Kasirajan; Julie A. Maupin‐Furlow
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Cysteine in cell culture media induces acidic IgG1 species by disrupting the disulfide bond network Biotechnol. Bioeng. (IF 4.002) Pub Date : 2020-11-17 Elke Prade; Anne Zeck; Fabian Stiefel; Andreas Unsoeld; David Mentrup; Erik Arango Gutierrez; Ingo H. Gorr
A high degree of charge heterogeneity is an unfavorable phenomenon commonly observed for therapeutic monoclonal antibodies (mAbs). Removal of these impurities during manufacturing often comes at the cost of impaired step yields. A wide spectrum of posttranslational and chemical modifications is known to modify mAb charge. However, a deeper understanding of underlying mechanisms triggering charged species
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Separation of nasopharyngeal epithelial cells from carcinoma cells on 3D scaffold platforms Biotechnol. Bioeng. (IF 4.002) Pub Date : 2020-11-26 W. G. Zhang; Z. Y. Liu; S. W. Pang
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Production of recombinant butyrylcholinesterase from transgenic rice cell suspension cultures in a pilot‐scale bioreactor Biotechnol. Bioeng. (IF 4.002) Pub Date : 2020-11-26 Kantharakorn Macharoen; Min Du; Seongwon Jung; Karen A. McDonald; Somen Nandi
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Professor Daniel I.C. Wang: A Legacy of Education, Innovation, Publication, and Leadership Biotechnol. Bioeng. (IF 4.002) Pub Date : 2020-12-08 Noubar B. Afeyan; Charles L. Cooney
Biochemical engineering at MIT emerged in the 1950's with a focus on the use of fermentation technology for traditional food and beverage processing and the increasing demands of antibiotics production.
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Improving viral filtration capacity in biomanufacturing processes using aggregate binding properties of polyamide‐6,6 Biotechnol. Bioeng. (IF 4.002) Pub Date : 2020-11-26 Vitali Stanevich; Abhishek Pachalla; Briana Nunez; Mark McInnes; Christopher Nieder; John Schreffler
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Questioning coverage values determined by 2D western blots: A critical study on the characterization of anti‐HCP ELISA reagents Biotechnol. Bioeng. (IF 4.002) Pub Date : 2020-11-26 Christina Seisenberger; Tobias Graf; Markus Haindl; Harald Wegele; Michael Wiedmann; Stefanie Wohlrab
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Lassa hemorrhagic shock syndrome‐on‐a‐chip Biotechnol. Bioeng. (IF 4.002) Pub Date : 2020-11-26 Huaqi Tang; Yasmine Abouleila; Alireza Mashaghi
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Development of a microbe domestication pod (MD Pod) for in situ cultivation of micro‐encapsulated marine bacteria Biotechnol. Bioeng. (IF 4.002) Pub Date : 2020-11-26 Tartela Alkayyali; Emily Pope; Sydney K. Wheatley; Christopher Cartmell; Bradley Haltli; Russell G. Kerr; Ali Ahmadi
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Scaffold‐free biofabrication of adipocyte structures with magnetic levitation Biotechnol. Bioeng. (IF 4.002) Pub Date : 2020-11-18 Oyku Sarigil; Muge Anil‐Inevi; Burcu Firatligil‐Yildirir; Yagmur Ceren Unal; Ozden Yalcin‐Ozuysal; Gulistan Mese; H. Cumhur Tekin; Engin Ozcivici
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Functional expression of eukaryotic cytochrome P450s in yeast Biotechnol. Bioeng. (IF 4.002) Pub Date : 2020-11-18 Lihong Jiang; Lei Huang; Jin Cai; Zhinan Xu; Jiazhang Lian
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Modeling of photosynthesis and respiration rate for microalgae–bacteria consortia Biotechnol. Bioeng. (IF 4.002) Pub Date : 2020-11-12 Ana Sánchez Zurano; Cintia Gómez Serrano; F. Gabriel Acién‐Fernández; Jose M. Fernández‐Sevilla; Emilio Molina‐Grima
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Development and application of a cultivation platform for mammalian suspension cell lines with single‐cell resolution Biotechnol. Bioeng. (IF 4.002) Pub Date : 2020-11-17 Julian Schmitz; Sarah Täuber; Christoph Westerwalbesloh; Eric von Lieres; Thomas Noll; Alexander Grünberger
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Studying endothelial cell shedding and orientation using adaptive perfusion‐culture in a microfluidic vascular chip Biotechnol. Bioeng. (IF 4.002) Pub Date : 2020-11-17 Xiang Zhang; Zhenxing Wang; Yu Shrike Zhang; Shujie Yan; Chuanyu Hou; Youping Gong; Jingjiang Qiu; Mo Chen; Qian Li