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Orna Therapeutics: circular logic Nat. Biotechnol. (IF 68.164) Pub Date : 2022-08-05
In the ascendant world of mRNA medicines, circular RNA shows promise as an alternative way for making proteins inside human cells.
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The digital phenotype of vaccination Nat. Biotechnol. (IF 68.164) Pub Date : 2022-08-03 Giorgio Quer, Eric J. Topol, Steven R. Steinhubl
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Increase vigilance against cyberattacks Nat. Biotechnol. (IF 68.164) Pub Date : 2022-08-03
The biotech sector must devote more resources to cybersecurity — especially those companies that are manufacturers of essential medical products.
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The BioInnovation Institute as a catalyst for European life science startup creation Nat. Biotechnol. (IF 68.164) Pub Date : 2022-08-03 Jens Nielsen, Markus J. Herrgård, Bobby Soni
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BEHAV3D: an imaging and transcriptomics platform that unravels T cell antitumor activity Nat. Biotechnol. (IF 68.164) Pub Date : 2022-07-28
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Precision cancer targeting with antibody pairs Nat. Biotechnol. (IF 68.164) Pub Date : 2022-07-25 Robert T. Lawrence, Peter D. Senter
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Ras-mutant cancers are sensitive to small molecule inhibition of V-type ATPases in mice Nat. Biotechnol. (IF 68.164) Pub Date : 2022-07-25 Bhairavi Tolani, Anna Celli, Yanmin Yao, Yong Zi Tan, Richard Fetter, Christina R. Liem, Adam J. de Smith, Thamiya Vasanthakumar, Paola Bisignano, Adam D. Cotton, Ian B. Seiple, John L. Rubinstein, Marco Jost, Jonathan S. Weissman
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Logic-gated antibody pairs that selectively act on cells co-expressing two antigens Nat. Biotechnol. (IF 68.164) Pub Date : 2022-07-25 Simone C. Oostindie, Derek A. Rinaldi, Gijs G. Zom, Michael J. Wester, Desiree Paulet, Kusai Al-Tamimi, Els van der Meijden, Jennifer R. Scheick, Tessa Wilpshaar, Bart de Jong, Marloes Hoff-van den Broek, Rachel M. Grattan, Janita J. Oosterhoff, Julie Vignau, Sandra Verploegen, Peter Boross, Frank J. Beurskens, Diane S. Lidke, Janine Schuurman, Rob N. de Jong
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Uncovering the mode of action of engineered T cells in patient cancer organoids Nat. Biotechnol. (IF 68.164) Pub Date : 2022-07-25 Johanna F. Dekkers, Maria Alieva, Astrid Cleven, Farid Keramati, Amber K. L. Wezenaar, Esmée J. van Vliet, Jens Puschhof, Peter Brazda, Inez Johanna, Angelo D. Meringa, Heggert G. Rebel, Maj-Britt Buchholz, Mario Barrera Román, Amber L. Zeeman, Sam de Blank, Domenico Fasci, Maarten H. Geurts, Annelisa M. Cornel, Else Driehuis, Rosemary Millen, Trudy Straetemans, Mara J. T. Nicolasen, Tineke Aarts-Riemens
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A new route to vaccines using PROTACs Nat. Biotechnol. (IF 68.164) Pub Date : 2022-07-22 Brad Gilbertson, Kanta Subbarao
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Wastewater is a robust proxy for monitoring circulating SARS-CoV-2 variants Nat. Biotechnol. (IF 68.164) Pub Date : 2022-07-21
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The trispecific DARPin ensovibep inhibits diverse SARS-CoV-2 variants Nat. Biotechnol. (IF 68.164) Pub Date : 2022-07-21 Sylvia Rothenberger, Daniel L. Hurdiss, Marcel Walser, Francesca Malvezzi, Jennifer Mayor, Sarah Ryter, Hector Moreno, Nicole Liechti, Andreas Bosshart, Chloé Iss, Valérie Calabro, Andreas Cornelius, Tanja Hospodarsch, Alexandra Neculcea, Thamar Looser, Anja Schlegel, Simon Fontaine, Denis Villemagne, Maria Paladino, Dieter Schiegg, Susanne Mangold, Christian Reichen, Filip Radom, Yvonne Kaufmann,
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Framework for multiplicative scaling of single-cell proteomics Nat. Biotechnol. (IF 68.164) Pub Date : 2022-07-18
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Engineering circular RNA for enhanced protein production Nat. Biotechnol. (IF 68.164) Pub Date : 2022-07-18 Robert Chen, Sean K. Wang, Julia A. Belk, Laura Amaya, Zhijian Li, Angel Cardenas, Brian T. Abe, Chun-Kan Chen, Paul A. Wender, Howard Y. Chang
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Viral variant-resolved wastewater surveillance of SARS-CoV-2 at national scale Nat. Biotechnol. (IF 68.164) Pub Date : 2022-07-18 Fabian Amman, Rudolf Markt, Lukas Endler, Sebastian Hupfauf, Benedikt Agerer, Anna Schedl, Lukas Richter, Melanie Zechmeister, Martin Bicher, Georg Heiler, Petr Triska, Matthew Thornton, Thomas Penz, Martin Senekowitsch, Jan Laine, Zsofia Keszei, Peter Klimek, Fabiana Nägele, Markus Mayr, Beatrice Daleiden, Martin Steinlechner, Harald Niederstätter, Petra Heidinger, Wolfgang Rauch, Christoph Scheffknecht
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Increasing the throughput of sensitive proteomics by plexDIA Nat. Biotechnol. (IF 68.164) Pub Date : 2022-07-14 Jason Derks, Andrew Leduc, Georg Wallmann, R. Gray Huffman, Matthew Willetts, Saad Khan, Harrison Specht, Markus Ralser, Vadim Demichev, Nikolai Slavov
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People Nat. Biotechnol. (IF 68.164) Pub Date : 2022-07-13
Recent moves of note in and around the biotech and pharma industries.
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RNA drugs lower lipoprotein(a) and genetically driven cholesterol Nat. Biotechnol. (IF 68.164) Pub Date : 2022-07-13
Companies are deploying siRNA and antisense oligonucleotides to tackle dangerously high cholesterol driven by genetics, betting that a wider population will benefit.
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Immuno-oncology checkpoint inhibitors Nat. Biotechnol. (IF 68.164) Pub Date : 2022-07-13
Recent patents relating to checkpoint inhibitors and their use in treating cancers.
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IL-2 upgrades show promise at ASCO Nat. Biotechnol. (IF 68.164) Pub Date : 2022-07-13
Companies pursue souped-up IL-2 candidates, undeterred by Nektar’s setback.
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What digitalization in biology R&D means for biotech companies and life scientists Nat. Biotechnol. (IF 68.164) Pub Date : 2022-07-13 Sybil Wong, Mingke Pan, Allen Shaw, Markus Gershater
The digitalization of R&D can potentially transform the life sciences, but it cannot succeed without experts collaborating to develop a vision of what a better working environment can look like.
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Standardized annotation of translated open reading frames Nat. Biotechnol. (IF 68.164) Pub Date : 2022-07-13 Jonathan M. Mudge, Jorge Ruiz-Orera, John R. Prensner, Marie A. Brunet, Ferriol Calvet, Irwin Jungreis, Jose Manuel Gonzalez, Michele Magrane, Thomas F. Martinez, Jana Felicitas Schulz, Yucheng T. Yang, M. Mar Albà, Julie L. Aspden, Pavel V. Baranov, Ariel A. Bazzini, Elspeth Bruford, Maria Jesus Martin, Lorenzo Calviello, Anne-Ruxandra Carvunis, Jin Chen, Juan Pablo Couso, Eric W. Deutsch, Paul Flicek
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Bluebird's CALD gene therapy poised for approval. Nat. Biotechnol. (IF 68.164) Pub Date : 2022-07-13
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Enhancing untargeted metabolomics using metadata-based source annotation Nat. Biotechnol. (IF 68.164) Pub Date : 2022-07-07 Julia M. Gauglitz, Kiana A. West, Wout Bittremieux, Candace L. Williams, Kelly C. Weldon, Morgan Panitchpakdi, Francesca Di Ottavio, Christine M. Aceves, Elizabeth Brown, Nicole C. Sikora, Alan K. Jarmusch, Cameron Martino, Anupriya Tripathi, Michael J. Meehan, Kathleen Dorrestein, Justin P. Shaffer, Roxana Coras, Fernando Vargas, Lindsay DeRight Goldasich, Tara Schwartz, MacKenzie Bryant, Gregory
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Revisiting checkpoint blockade Nat. Biotechnol. (IF 68.164) Pub Date : 2022-07-05
After over a decade of experience with immune checkpoint inhibitors in oncology, more effort needs to be spent unraveling why some patients respond — and why the majority do not — and integrating knowledge about biomarkers into patient selection in trials.
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Identifying drug combinations that enhance treatment responses mediated by the tumor microenvironment Nat. Biotechnol. (IF 68.164) Pub Date : 2022-07-04
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A multiplex implantable microdevice assay identifies synergistic combinations of cancer immunotherapies and conventional drugs Nat. Biotechnol. (IF 68.164) Pub Date : 2022-07-04 Zuzana Tatarova, Dylan C. Blumberg, James E. Korkola, Laura M. Heiser, John L. Muschler, Pepper J. Schedin, Sebastian W. Ahn, Gordon B. Mills, Lisa M. Coussens, Oliver Jonas, Joe W. Gray
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Generation of a live attenuated influenza A vaccine by proteolysis targeting Nat. Biotechnol. (IF 68.164) Pub Date : 2022-07-04 Longlong Si, Quan Shen, Jing Li, Li Chen, Jinying Shen, Xue Xiao, Haiqing Bai, Tang Feng, Adam Yongxin Ye, Le Li, Chunhe Zhang, Zhen Li, Ping Wang, Crystal Yuri Oh, Atiq Nurani, Siwen Niu, Chengxin Zhang, Xiaoqiong Wei, Wanqiong Yuan, Hao Liao, Xiaojie Huang, Ning Wang, Wen-xia Tian, Hongwei Tian, Li Li, Xiaoheng Liu, Roberto Plebani
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Synthetic memory circuits for stable cell reprogramming in plants Nat. Biotechnol. (IF 68.164) Pub Date : 2022-07-04 James P. B. Lloyd, Florence Ly, Patrick Gong, Jahnvi Pflueger, Tessa Swain, Christian Pflueger, Elliott Fourie, Muhammad Adil Khan, Brendan N. Kidd, Ryan Lister
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Frequent aneuploidy in primary human T cells after CRISPR–Cas9 cleavage Nat. Biotechnol. (IF 68.164) Pub Date : 2022-06-30 Alessio David Nahmad, Eli Reuveni, Ella Goldschmidt, Tamar Tenne, Meytal Liberman, Miriam Horovitz-Fried, Rami Khosravi, Hila Kobo, Eyal Reinstein, Asaf Madi, Uri Ben-David, Adi Barzel
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Profiling the total single-cell transciptome using droplet microfluidics Nat. Biotechnol. (IF 68.164) Pub Date : 2022-06-28
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Archiving the genomic and genetic resources of glaciers Nat. Biotechnol. (IF 68.164) Pub Date : 2022-06-27
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High-throughput total RNA sequencing in single cells using VASA-seq Nat. Biotechnol. (IF 68.164) Pub Date : 2022-06-27 Fredrik Salmen, Joachim De Jonghe, Tomasz S. Kaminski, Anna Alemany, Guillermo E. Parada, Joe Verity-Legg, Ayaka Yanagida, Timo N. Kohler, Nicholas Battich, Floris van den Brekel, Anna L. Ellermann, Alfonso Martinez Arias, Jennifer Nichols, Martin Hemberg, Florian Hollfelder, Alexander van Oudenaarden
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A genome and gene catalog of glacier microbiomes Nat. Biotechnol. (IF 68.164) Pub Date : 2022-06-27 Yongqin Liu, Mukan Ji, Tao Yu, Julian Zaugg, Alexandre M. Anesio, Zhihao Zhang, Songnian Hu, Philip Hugenholtz, Keshao Liu, Pengfei Liu, Yuying Chen, Yingfeng Luo, Tandong Yao
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Plant hormone sensors as scaffolds for biosensor design Nat. Biotechnol. (IF 68.164) Pub Date : 2022-06-20
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Genome-wide mapping of somatic mutation rates uncovers drivers of cancer Nat. Biotechnol. (IF 68.164) Pub Date : 2022-06-20 Maxwell A. Sherman, Adam U. Yaari, Oliver Priebe, Felix Dietlein, Po-Ru Loh, Bonnie Berger
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Rapid biosensor development using plant hormone receptors as reprogrammable scaffolds Nat. Biotechnol. (IF 68.164) Pub Date : 2022-06-20 Jesús Beltrán, Paul J. Steiner, Matthew Bedewitz, Shuang Wei, Francis C. Peterson, Zongbo Li, Brigid E. Hughes, Zachary Hartley, Nicholas R. Robertson, Angélica V. Medina-Cucurella, Zachary T. Baumer, Alison C. Leonard, Sang-Youl Park, Brian F. Volkman, Dmitri A. Nusinow, Wenwan Zhong, Ian Wheeldon, Sean R. Cutler, Timothy A. Whitehead
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Smartphone apps in the COVID-19 pandemic Nat. Biotechnol. (IF 68.164) Pub Date : 2022-06-20 Jay A. Pandit, Jennifer M. Radin, Giorgio Quer, Eric J. Topol
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Measuring SARS-CoV-2 T cell immunity with a scalable qPCR-based assay Nat. Biotechnol. (IF 68.164) Pub Date : 2022-06-17
We developed two quantitative PCR-based assays to detect SARS-CoV-2-specific T cell immunity: qTACT and dqTACT. The assays quantify CXCL10 mRNA, after incubation of whole blood with viral peptides, as a proxy of an antigen-specific T cell response, and will allow population-level monitoring of cellular immunity to SARS-CoV-2.
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CRISPR technology Nat. Biotechnol. (IF 68.164) Pub Date : 2022-06-15
Recent patents relating to CRISPR methods and compositions for gene editing and therapeutic use.
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Topical herpesvirus gene therapy enters final lap Nat. Biotechnol. (IF 68.164) Pub Date : 2022-06-15
The first herpesvirus replacement gene therapy for epidermolysis bullosa, a blistering skin condition, approaches the clinic.
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People Nat. Biotechnol. (IF 68.164) Pub Date : 2022-06-15
Recent moves of note in and around the biotech and pharma industries.
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Unconventional γδ T cells ‘the new black’ in cancer therapy Nat. Biotechnol. (IF 68.164) Pub Date : 2022-06-15
A new era of cancer immunotherapy beckons as γδ T cell trials enter final stage.
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Vascepa patents under legal scrutiny Nat. Biotechnol. (IF 68.164) Pub Date : 2022-06-15 Gregory Curfman, Justin Cole
Even if Amarin prevails in its patent infringement lawsuit, its key patents on Vascepa are likely to be ruled invalid in ongoing litigation, opening the way for generic formulations.
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A virtual innovation bootcamp to remotely connect and empower students to solve COVID-19-related medical problems Nat. Biotechnol. (IF 68.164) Pub Date : 2022-06-15 Mario V. Russo, Abhinav J. Appukutty, Aadit P. Shah, Harsha K. Mohan, Andy G. S. Daniel, Andrew Pack, Richard Xie
An intensive program supplements student education and collaboration while narrowing the disconnect between rising healthcare needs and trainees with the technical expertise to tackle pandemic-related challenges.
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Towards the non-invasive imaging of brain networks and functions at high resolution Nat. Biotechnol. (IF 68.164) Pub Date : 2022-06-13
A new study describes a microscope combining three-photon excitation and adaptive optics capable of high-resolution in vivo imaging of fine neuronal structures in the mouse cortex through the intact skull. The authors demonstrate the use of this platform to guide precise laser-mediated microsurgery and for accurate and sensitive functional calcium imaging.
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A red light–responsive photoswitch for deep tissue optogenetics Nat. Biotechnol. (IF 68.164) Pub Date : 2022-06-13 Yuto Kuwasaki, Kazushi Suzuki, Gaigai Yu, Shota Yamamoto, Takahiro Otabe, Yuki Kakihara, Michiru Nishiwaki, Keita Miyake, Keiji Fushimi, Ramsey Bekdash, Yoshihiro Shimizu, Rei Narikawa, Takahiro Nakajima, Masayuki Yazawa, Moritoshi Sato
Red light penetrates deep into mammalian tissues and has low phototoxicity, but few optogenetic tools that use red light have been developed. Here we present MagRed, a red light–activatable photoswitch that consists of a red light–absorbing bacterial phytochrome incorporating a mammalian endogenous chromophore, biliverdin and a photo-state-specific binder that we developed using Affibody library selection
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Estimation of tumor cell total mRNA expression in 15 cancer types predicts disease progression Nat. Biotechnol. (IF 68.164) Pub Date : 2022-06-13 Shaolong Cao, Jennifer R. Wang, Shuangxi Ji, Peng Yang, Yaoyi Dai, Shuai Guo, Matthew D. Montierth, John Paul Shen, Xiao Zhao, Jingxiao Chen, Jaewon James Lee, Paola A. Guerrero, Nicholas Spetsieris, Nikolai Engedal, Sinja Taavitsainen, Kaixian Yu, Julie Livingstone, Vinayak Bhandari, Shawna M. Hubert, Najat C. Daw, P. Andrew Futreal, Eleni Efstathiou, Bora Lim, Andrea Viale, Jianjun Zhang, Matti Nykter
Single-cell RNA sequencing studies have suggested that total mRNA content correlates with tumor phenotypes. Technical and analytical challenges, however, have so far impeded at-scale pan-cancer examination of total mRNA content. Here we present a method to quantify tumor-specific total mRNA expression (TmS) from bulk sequencing data, taking into account tumor transcript proportion, purity and ploidy
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Rapid, scalable assessment of SARS-CoV-2 cellular immunity by whole-blood PCR Nat. Biotechnol. (IF 68.164) Pub Date : 2022-06-13 Megan Schwarz, Denis Torre, Daniel Lozano-Ojalvo, Anthony T. Tan, Tommaso Tabaglio, Slim Mzoughi, Rodrigo Sanchez-Tarjuelo, Nina Le Bert, Joey Ming Er Lim, Sandra Hatem, Kevin Tuballes, Carmen Camara, Eduardo Lopez-Granados, Estela Paz-Artal, Rafael Correa-Rocha, Alberto Ortiz, Marcos Lopez-Hoyos, Jose Portoles, Isabel Cervera, Maria Gonzalez-Perez, Irene Bodega-Mayor, Patricia Conde, Jesús Oteo-Iglesias
Fast, high-throughput methods for measuring the level and duration of protective immune responses to SARS-CoV-2 are needed to anticipate the risk of breakthrough infections. Here we report the development of two quantitative PCR assays for SARS-CoV-2-specific T cell activation. The assays are rapid, internally normalized and probe-based: qTACT requires RNA extraction and dqTACT avoids sample preparation
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Deep tissue multi-photon imaging using adaptive optics with direct focus sensing and shaping Nat. Biotechnol. (IF 68.164) Pub Date : 2022-06-13 Zhongya Qin, Zhentao She, Congping Chen, Wanjie Wu, Jackie K. Y. Lau, Nancy Y. Ip, Jianan Y. Qu
High-resolution optical imaging deep in tissues is challenging because of optical aberrations and scattering of light caused by the complex structure of living matter. Here we present an adaptive optics three-photon microscope based on analog lock-in phase detection for focus sensing and shaping (ALPHA-FSS). ALPHA-FSS accurately measures and effectively compensates for both aberrations and scattering
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CRISPR comes a-knock-in to reprogram antibodies in vivo Nat. Biotechnol. (IF 68.164) Pub Date : 2022-06-09 Harald Hartweger, Michel C. Nussenzweig
Reprogramming of antibody responses in mice is achieved via adeno-associated virus delivery of SaCas9, single guide RNA (sgRNA) and a repair template targeting immunoglobulin genes.
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In vivo engineered B cells secrete high titers of broadly neutralizing anti-HIV antibodies in mice Nat. Biotechnol. (IF 68.164) Pub Date : 2022-06-09 Alessio D. Nahmad, Cicera R. Lazzarotto, Natalie Zelikson, Talia Kustin, Mary Tenuta, Deli Huang, Inbal Reuveni, Daniel Nataf, Yuval Raviv, Miriam Horovitz-Fried, Iris Dotan, Yaron Carmi, Rina Rosin-Arbesfeld, David Nemazee, James E. Voss, Adi Stern, Shengdar Q. Tsai, Adi Barzel
Transplantation of B cells engineered ex vivo to secrete broadly neutralizing antibodies (bNAbs) has shown efficacy in disease models. However, clinical translation of this approach would require specialized medical centers, technically demanding protocols and major histocompatibility complex compatibility of donor cells and recipients. Here we report in vivo B cell engineering using two adeno-associated
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Lipophilic siRNA conjugates yield durable silencing in extrahepatic tissues Nat. Biotechnol. (IF 68.164) Pub Date : 2022-06-08
RNA interference (RNAi) therapeutics can silence disease-causing gene transcripts, but extrahepatic delivery has been challenging. Conjugating short interfering RNAs (siRNAs) to a lipophilic alkyl chain enabled safe delivery and long-term mRNA silencing in the brain, eye and lung in animal models, thereby opening new applications for RNAi therapeutics.
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Knock-in on CRISPR’s door Nat. Biotechnol. (IF 68.164) Pub Date : 2022-06-07
Interest is growing in genome-editing tools that can insert large chunks of DNA into the genome — and avoid the double-strand breaks associated with CRISPR–Cas9 genotoxicity.
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Visualization of living cells and tissues in many colors Nat. Biotechnol. (IF 68.164) Pub Date : 2022-06-06
Existing technologies can map the intricate spatial distribution of biomolecules and cell types within tissues, but not in specimens that remain alive and intact. This study introduces scission-accelerated fluorophore exchange (SAFE) bioorthogonal imaging tools that enable living cells and tissues to be deeply and serially profiled, revealing their biological dynamics across both space and time.
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First liver transplantation using a graft maintained ex vivo for several days Nat. Biotechnol. (IF 68.164) Pub Date : 2022-06-06
Our multidisciplinary team used novel perfusion technology to successfully keep an injured liver graft discarded by all other centers alive for several days, allowing proper graft evaluation and repair. The graft was successfully transplanted into a sick patient, who recovered quickly and enjoyed a normal life at one-year follow up.