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Isolation and characterization of a multidrug-resistant Staphylococcus aureus infecting phage and its therapeutic use in mice FEMS Microbiol. Lett. (IF 2.2) Pub Date : 2024-09-13 Zhen Xiao, Hongyi Xu, Juan Wang, Xueyuan Hu, Xiumei Huang, Shiping Song, Qingqing Zhang, Yanxin Liu, Yaopeng Liu, Na Liu, Junhui Liu, Ge Zhao, Xiyue Zhang, Yuehua Li, Jianmei Zhao, Junwei Wang, Huanqi Liu, Lin Wang, Zhina Qu
In recent years, the emergence of multidrug-resistant bacteria has limited the selection of drugs for treating bacterial infections, reduced clinical efficacy, and increased treatment costs and mortality. It is urgent to find alternative antibiotics. In order to explore a new method for controlling methicillin resistant Staphylococcus aureus (S. aureus) , this study isolated and purified a multi drug
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Organization, Conservation, and Diversity of Biosynthetic Gene Clusters in Bacillus sp. BH32 and Its Closest Relatives in the Bacillus cereus Group FEMS Microbiol. Lett. (IF 2.2) Pub Date : 2024-09-09 Hadj Ahmed Belaouni, Amine Yekkour, Abdelghani Zitouni, Atika Meklat
This study explores the organization, conservation, and diversity of biosynthetic gene clusters (BGCs) among Bacillus sp. strain BH32, a plant-beneficial bacterial endophyte, and its closest non-type Bacillus cereus group strains. BGC profiles were predicted for each of the 17 selected strains using antiSMASH, resulting in the detection of a total of 198 BGCs. We quantitatively compared the BGCs and
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Carbapenemase and extended spectrum beta-lactamase producing bacteria in waters originating from a single landfill in Slovenia FEMS Microbiol. Lett. (IF 2.2) Pub Date : 2024-09-01 Valerija Tkalec, Polona Lindic, Tatjana Jursa, Hermina Ivanusa Sket, Leon Maric, Mojca Cimerman, Maja Rupnik, Andrej Golle
Groundwater, rainwater, and leachate associated with a single landfill were analysed to detect extended spectrum beta-lactamase (ESBL) producing and carbapenemase (CP) producing bacteria. After cultivation on three commercial selective-differential media, 240 bacterial isolates were obtained and identified by MALDI-TOF MS. Isolates from clinically relevant species were further genotyped by Enterobacterial
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Antimicrobial susceptibility of commensal Neisseria spp. In parents and their children in Belgium: a cross-sectional survey FEMS Microbiol. Lett. (IF 2.2) Pub Date : 2024-08-30 Saïd Abdellati, Zina Gestels, Jolein Gyonne Elise Laumen, Christophe Van Dijck, Irith De Baetselier, Tessa de Block, Dorien Van den Bossche, Thibaut Vanbaelen, Izumo Kanesaka, Sheeba Santhini Manoharan-Basil, Chris Kenyon
Background Commensal Neisseria species are part of the oropharyngeal microbiome and play an important role in nitrate reduction and protecting against colonization by pathogenic bacteria. They do, however, also serve as a reservoir of antimicrobial resistance. Little is known about the prevalence of these species in the general population, how this varies by age and how antimicrobial susceptibility
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The influence of cigR gene on the pathogenicity of Salmonella paratyphi A in vitro and in vivo. FEMS Microbiol. Lett. (IF 2.2) Pub Date : 2024-01-09 Junlei Yin,Lijun Wang,Ronghua Shen,Jinjiao He,Shaozu Li,Huajian Wang,Zhao Cheng
Salmonella Paratyphi A is the causative agent of paratyphoid fever A which is a serious threat to human health in many countries. The cigR gene located in Salmonella pathogenicity island 3 is a type III secretion system 2 effector gene. However, the influence of cigR gene on the pathogenicity of Salmonella Paratyphi A remains unclear. Here, a cigR gene deletion mutant of Salmonella Paratyphi A was
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A universal and constant rate of gene content change traces pangenome flux to LUCA. FEMS Microbiol. Lett. (IF 2.2) Pub Date : 2024-01-09 Katharina Trost,Michael R Knopp,Jessica L E Wimmer,Fernando D K Tria,William F Martin
Prokaryotic genomes constantly undergo gene flux via lateral gene transfer, generating a pangenome structure consisting of a conserved core genome surrounded by a more variable accessory genome shell. Over time, flux generates change in genome content. Here, we measure and compare the rate of genome flux for 5655 prokaryotic genomes as a function of amino acid sequence divergence in 36 universally
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Heterologous expression and purification of the phage lysin-like bacteriocin LysL from lactococcus lactis LAC460 FEMS Microbiol. Lett. (IF 2.2) Pub Date : 2024-08-16 Samira Mokhtari, Per E J Saris, Timo M Takala
The wild-type Lactococcus lactis strain LAC460 produces two bacteriocin-like phage lysins, LysL and LysP. This study aimed to produce and secrete LysL in various heterologous hosts and an In vitro cell-free expression system for further functional studies. Initially, the lysL gene from L. lactis LAC460 was cloned into Lactococcus cremoris NZ9000 and L. lactis N8 strains, with and without the usp45
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Antibacterial mode of action of garviecin LG34 against Gram-negative bacterium Salmonella typhimurium FEMS Microbiol. Lett. (IF 2.2) Pub Date : 2024-08-13 Yurong Gao, Depeng Li
Garviecin LG34 produced by Lactococcus garvieae LG34 exhibits wide-spectrum antibacterial activity against both Gram-positive and Gram-negative bacteria. This work aimed at clarifying the antibacterial mode of action of garviecin LG34 against Gram-negative bacterium S. typhimurium. To determine the concentration for the bacteriocin antimicrobial mode experiments, the minimum concentration of garviecin
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Metabolic reprogramming in the food-borne pathogen Listeria monocytogenes as a critical defence against acid stress FEMS Microbiol. Lett. (IF 2.2) Pub Date : 2024-08-08 Jialun Wu, Chuhan Wang, Conor O'Byrne
The ability to sense and respond effectively to acidic stress is important for microorganisms to survive and proliferate in fluctuating environments. As specific metabolic activities can serve to buffer the cytoplasmic pH, microorganisms re-wire their metabolism to favour these reactions and thereby mitigate acid stress. The orally-acquired pathogen Listeria monocytogenes exploits alternative metabolic
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Impact of chitin-derived β-N-acetyl-D-glucosaminyl-(1,4)-D-glucosamine on chitinase up-regulation in Shewanella baltica FEMS Microbiol. Lett. (IF 2.2) Pub Date : 2024-08-07 Takako Hirano, Masahiro Yokoyama, Masafumi Ikejima, Haruka Shiraishi, Wataru Hakamata, Toshiyuki Nishio
The first steps in chitin degradation in marine bacteria involve chitinase, which produces N,N′-diacetylchitobiose (GlcNAc)2 from chitin. Moreover, in Vibrio bacteria, chitinase activity is enhanced by heterodisaccharide β-N-acetyl-D-glucosaminyl-(1,4)-D-glucosamine (GlcNAc-GlcN) produced from (GlcNAc)2 by chitin oligosaccharide deacetylase (COD). However, the role of COD in other marine bacteria,
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Identification of mutations resulting in de-repression of the intermedilysin gene by sequential mutagenesis of its promoter region in Streptococcus intermedius FEMS Microbiol. Lett. (IF 2.2) Pub Date : 2024-08-02 Toshifumi Tomoyasu, Atsushi Tabata, Hideaki Nagamune
Streptococcus intermedius secretes the human-specific cytolysin intermedilysin (ILY), a crucial factor in the pathogenicity of this bacterium. Previously, we reported that a lactose phosphotransferase repressor (LacR) represses ily expression and that its mutation increases ILY production. Interestingly, UNS40, a strain isolated from a liver abscess, produces high levels of ILY despite the absence
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Genomic analysis of foodborne Staphylococcus aureus obtained from unannounced food inspections between 2012 and 2021 in East China FEMS Microbiol. Lett. (IF 2.2) Pub Date : 2024-08-01 Chu Lu, Wenjie Xiao, Haoyun Yu, Weiyi Song, Zhemin Zhou, Ning Dong, Zhihai Fan, Heng Li
Staphylococcus aureus is a significant cause of foodborne illness in China. Our investigation concentrated on the genetic characterization of foodborne S. aureus identified during unannounced inspections conducted in Suzhou from 2012 to 2021. Dominant clones included CC1, CC398, CC188, and CC7, with CC398 notably increasing in 2020–2021. The isolates commonly contained 1–3 plasmids, with rep5a (48
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Influence of furfural on the physiology of Acinetobacter baylyi ADP1 FEMS Microbiol. Lett. (IF 2.2) Pub Date : 2024-07-30 José Eduardo Arteaga, Ernesto Rivera-Becerril, Sylvie Le Borgne, Juan-Carlos Sigala
Pretreatment of lignocellulosic biomass produces growth inhibitory substances such as furfural which is toxic to microorganisms. Acinetobacter baylyi ADP1 cannot use furfural as a carbon source, instead it biotransforms this compound into difurfuryl ether using the NADH-dependent dehydrogenases AreB and FrmA during aerobic acetate catabolism. However, NADH consumption for furfural biotransformation
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Analysis of CenKR essentiality in sinorhizobium meliloti and its activity at a target gene promoter in vivo FEMS Microbiol. Lett. (IF 2.2) Pub Date : 2024-07-25 Isabela R Freire, Eukene O Bensig, ZiYu Kuang, Shawn R MacLellan
The two-component regulatory system CenK-CenR has recently emerged as a regulator of cell envelope and cell division processes in the alpha-proteobacteria. In Sinorhizobium meliloti, CenK-CenR regulates the expression of SrlA, a thioredoxin-domain protein of unknown function. Deletion of srlA causes sensitivity to salt and oxidizing agents on solid growth medium. In this work, we report that the response
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Expression regulation of type III secretion system 2 in Vibrio parahaemolyticus by catabolite activator protein FEMS Microbiol. Lett. (IF 2.2) Pub Date : 2024-07-24 Tomotaka Tanabe, Mitsuki Tsukamoto, Mahiro Shioda, Kenjiro Nagaoka, Tatsuya Funahashi
Vibrio parahaemolyticus has two sets of type III secretion systems that are major pathogenic factors: T3SS1 (cytotoxicity) and T3SS2 (enterotoxicity). V. parahaemolyticus mainly colonizes the distal small intestine after oral infection and may be exposed to carbon-limiting stress due to the lack of readily available carbohydrates in this environment. Catabolite activator protein (CAP), a transcription
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Enzymatic characterization of five thioredoxins and a thioredoxin reductase from Myxococcus xanthus FEMS Microbiol. Lett. (IF 2.2) Pub Date : 2024-07-20 Ryota Tanifuji, Yoshio Kimura
Thioredoxin (Trx) is a disulfide-containing redox protein that functions as a disulfide oxidoreductase. Myxococcus xanthus contains five Trxs (Trx1–Trx5) and one Trx reductase (TrxR). Trxs typically have a CGPC active-site motif; however, M. xanthus Trxs have slightly different active-site sequences, with the exception of Trx4. The five Trxs of M. xanthus exhibited reduced activities against insulin
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Tryptophan transport gene inactivation promotes the development of antibiotic resistance in Escherichia coli FEMS Microbiol. Lett. (IF 2.2) Pub Date : 2024-07-19 Beibei Hou, Jianxiao Song, Huan Wang, Nan Ye, Rui-Wu Wang
Indole serves as a signaling molecule that could regulate different bacterial physiological processes, including antibiotic resistance through biofilm formation and drug efflux pump activity. In Escherichia coli (E. coli), indole is produced through the tryptophan pathway, which involves three permeases (Mtr, AroP, and TnaB) that can transport the amino acid tryptophan. Although these permeases paly
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Isolation, identification and biological characterization of bacterial endophytes isolated from Gunnera Perpensa L. FEMS Microbiol. Lett. (IF 2.2) Pub Date : 2024-07-18 Siphiwe Godfrey Mahlangu, Nodumo Zulu, Mahloro Hope Serepa-Dlamini, Siew Leng Tai
In the present study, eleven endophytic bacterial strains, Herbaspirillum sp. (GP-SGM1, GP-SGM2, GP-SGM3, GP-SGM11), Pseudomonas sp. (GP-SGM4, GP-SGM5), Novosphingobium sp. GP-SGM6, Chryseobacterium sp. GP-SGM7, Labedella sp. GP-SGM8, Brevibacterium sp. GP-SGM9, and Pseudomonas sp. GP-SGM10, were isolated from the rhizomes of Gunnera perpensa L. The growth kinetics, assessed through maximum growth
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Regulatory role of VvsB protein on serine protease activity of VvsA in Vibrio vulnificus FEMS Microbiol. Lett. (IF 2.2) Pub Date : 2024-07-17 Tomoka Kawase, Anusuya Debnath, Keinosuke Okamoto
Background Vibrio vulnificus NCIMB2137, a Gram-negative, metalloprotease negative estuarine strain was isolated from a diseased eel. A 45 kDa chymotrypsin-like alkaline serine protease known as VvsA has been recently reported as one of the major virulence factor responsible for the pathogenesis of this strain. The vvsA gene along with a downstream gene vvsB, whose function is still unknown constitute
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A modular inquiry-based semester theme that integrates data science education and bioinformatics in protein structure function courses FEMS Microbiol. Lett. (IF 2.2) Pub Date : 2024-07-16 Zareen Amtul, Forough Firoozbakht, Iman Rezaeian, Arham A Aziz, Padmini Gehlaut
Background With an exponential growth in biological data and computing power, familiarity with bioinformatics has become a demanding and popular skill set both in academia and industry. There is a need to increase students’ competencies to be able to take on bioinformatic careers, to get them familiarized with scientific professions in data science and the academic training required to pursue them
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High-throughput method for screening pendimethalin-degrading bacteria from one microbial bank FEMS Microbiol. Lett. (IF 2.2) Pub Date : 2024-07-08 Ya-Peng Peng, Liang Ma, Ying Huang, Ming-He Mo, Jian-Jin Liu, Tong Liu
The extensive use of chemical pesticides, such as herbicides, has resulted in significant environmental pollution. Microbial degradation represents a crucial approach for managing this pesticide-associated pollution, with enrichment culturing serving as a method for isolating pesticide-degrading microorganisms. However, the efficiency of this strategy is limited, often yielding only a few isolated
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The effectiveness of endolysin ENDO-1252 from Salmonella bacteriophage-1252 against nontyphoidal Salmonella enterica FEMS Microbiol. Lett. (IF 2.2) Pub Date : 2024-07-06 Chuan-Wei Tung, Dita Julianingsih, Christa Canagarajah, George Sellers, Aaron Scriba, Zabdiel Alvarado-Martínez, Zajeba Tabashsum, Debabrata Biswas
Salmonella enterica (S. enterica) is the most common food and waterborne pathogen worldwide. The growing trend of antibiotic-resistant S. enterica poses severe healthcare threats. As an alternative antimicrobial agent, bacteriophage-encoded endolysins (endolysin) are a potential agent in controlling S. enterica infection. Endolysins are enzymes that particularly target the peptidoglycan layer of bacterial
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Dynamic responses of Salmonella Typhimurium to re-exposure to sublethal ciprofloxacin FEMS Microbiol. Lett. (IF 2.2) Pub Date : 2024-06-27 Jiseok Yi, Junhwan Kim, Juhee Ahn
This study was designed to evaluate the history-dependent behaviors of Salmonella Typhimurium re-exposed to sublethal levels of ciprofloxacin. The S. Typhimurium cells were pre-exposed to 0 (CON), 1/16 (LOW), 1/8 (MED), and 1/4 (HIGH) MICs of ciprofloxacin, followed by re-exposure to the same concentrations. The bacterial growth, post-antibiotic effect (PAE), relative fitness, and swimming motility
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PqqF inhibits T6SS secretion by decreasing the pH in Serratia marcescens FS14 FEMS Microbiol. Lett. (IF 2.2) Pub Date : 2024-06-22 Fengyu Jia, Xuede Peng, Xiaomei Yang, Shenshen Qiu, Shanshan Jia, Tingting Ran, Weiwu Wang, Dongqing Xu
Pyrroloquinoline quinone (PQQ) is a redox cofactor with numerous important physiological functions, and the type VI secretion system (T6SS) is commonly found in Gram-negative bacteria and plays important roles in physiological metabolism of the bacteria. In this study, we found that the deletion of pqqF enhanced the secretion of Hcp-1 in Serratia marcesens FS14 in M9 medium. Transcriptional analysis
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Effects of sample storage temperature and duration on the detection of foliar endophytes of tea plants (Camellia sinensis L.) in summer and winter FEMS Microbiol. Lett. (IF 2.2) Pub Date : 2024-06-13 Phyu Mar Win, Norihisa Matsushita, Kenji Fukuda
Seasonal changes in the diversity of tea plant endophytic fungi and the effects of sample storage conditions on detection were analyzed. Tea leaves were collected from the Saitama Tea Research Institute in Japan during winter (January 2020) and summer (August 2020). The effects of storage temperature (5 °C, 10 °C, 20 °C, 25 °C, and 30 °C) and durations (1, 2, 3, 4, 5, 6, and 7 days) on endophytic fungal
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Contributing to an inclusive education for neurodivergent students: sharing reflections, practices and experiences (revised – no track changes 24 May) FEMS Microbiol. Lett. (IF 2.2) Pub Date : 2024-06-12 Giorgia Pigato
It is estimated that 1 in 7 individuals, more than 15% of the population in the UK, are neurodivergent. In recent years, there has been a notable increase in university students disclosing disabilities, specific learning difficulties, or mental health conditions. Despite this, students with disabilities and learning differences often experience lower levels of well-being compared to their peers, and
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The MATH test. A three-phase assay? FEMS Microbiol. Lett. (IF 2.2) Pub Date : 2024-06-12 Jesús A Salas-Tovar, Sarai Escobedo-García, Guadalupe I Olivas, Carlos H Acosta-Muñiz, Federico Harte, David R Sepulveda
This study aimed to investigating the possible interference caused by glass test tubes on the quantification of bacterial adhesion to hydrocarbons by the MATH test. The adhesion of four bacteria to hexadecane and to glass test tubes was evaluated employing different suspending polar phases. The role of the ionic strength of the polar phase regarding adhesion to glassware was investigated. Within the
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Impacts of sample handling and storage conditions on archiving physiologically active soil microbial communities FEMS Microbiol. Lett. (IF 2.2) Pub Date : 2024-06-12 Marcus T Brock, Hilary G Morrison, Loïs Maignien, Cynthia Weinig
Soil microbial communities are fundamental to ecosystem processes and plant growth, yet community composition is seasonally and successionally dynamic, which interferes with long-term iterative experimentation of plant-microbe interactions. We explore how soil sample handling (e.g. filtering) and sample storage conditions impact the ability to revive the original, physiologically active, soil microbial
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Rapid adaptation of the rainbow trout intestinal microbiota to the use of a high-starch 100% plant-based diet FEMS Microbiol. Lett. (IF 2.2) Pub Date : 2024-06-09 Raphaël Defaix, Jep Lokesh, Jessica Calo, Sandra Biasutti, Anne Surget, Frédéric Terrier, José Luis Soengas, Stéphane Panserat, Karine Ricaud
Short-term adaptation of the microbiota could promote nutrient degradation and the host health. While numerous studies are currently undertaking feeding trials using sustainable diets for the aquaculture industry, the extent to which the microbiota adapts to these novel diets is poorly described. The incorporation of carbohydrates (CHO) within a 100% plant-based diet could offer a novel, cost-effective
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Increasing community prevalence of extended-spectrum beta-lactamase-producing Escherichia coli in urine is associated with increasing district-level antibiotic consumption FEMS Microbiol. Lett. (IF 2.2) Pub Date : 2024-06-01 Larissa Hermann de Souza Nunes, Victoria Stadler Tasca Ribeiro, Rafael Buttini Salviato, Ana Paula de Andrade, Paula Hansen Suss, Fernando José Vicenzi, Adriano Akira Ferreira Hino, João Paulo Telles, Felipe Francisco Tuon
Introduction This study aimed to analyze ESBL-producing Escherichia coli prevalence in urine samples collected between 2011–2019 in Curitiba, a large city in Brazil, and relating it to antibiotic consumption and sanitary conditions. Methods This is a longitudinal study correlating prevalence of ESBL-producing E. coli isolates from urine samples with district-level antibiotic consumption and sociodemographic
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Using “adopt a bacterium” as an E-learning tool for simultaneously teaching microbiology to different health-related university courses FEMS Microbiol. Lett. (IF 2.2) Pub Date : 2024-05-23 Hadassa Cristhina de Azevedo Soares dos Santos, Bárbara Rodrigues Cintra Armellini, Giovanna Lello Naves, Vanessa Bueris, Ana Carolina Ramos Moreno, Rita de Cássia Café Ferreira
The COVID-19 pandemic has posed challenges for education, particularly in undergraduate teaching. In this study, we report on the experience of how a private university successfully addressed this challenge through an active methodology applied to a microbiology discipline offered remotely to students from various health-related courses (veterinary, physiotherapy, nursing, biomedicine, and nutrition)
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Evaluation and comparison of colorimetric outputs for yeast-based biosensors in laboratory and point-of-use settings FEMS Microbiol. Lett. (IF 2.2) Pub Date : 2024-05-22 Andrea Clausen Lind, Florian David, Verena Siewers
Recent research has shown the potential of yeast-based biosensors (YBBs) for point-of-use detection of pathogens and target molecules in saliva, blood, and urine samples. The choice of output can greatly affect the sensitivity, dynamic range, detection time, and ease-of-use of a sensor. For visual detection without the need for additional reagents or machinery, colorimetric outputs have shown great
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Lactate metabolism promotes in vivo fitness during Acinetobacter baumannii infection FEMS Microbiol. Lett. (IF 2.2) Pub Date : 2024-05-09 Faye C Morris, Yan Jiang, Ying Fu, Xenia Kostoulias, Gerald L Murray, Yusong Yu, Anton Y Peleg
Acinetobacter baumannii is one of the most prevalent causes of nosocomial infections worldwide. However, a paucity of information exists regarding the connection between metabolic capacity and in vivo bacterial fitness. Elevated lactate is a key marker of severe sepsis. We have previously shown that the putative A. baumannii lactate permease gene, lldP, is upregulated during in vivo infection. Here
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Sensitivity of Salmonella Typhimurium to nisin in vitro and in orange juice under refrigeration FEMS Microbiol. Lett. (IF 2.2) Pub Date : 2024-05-08 Carla Gabriela Fernandes da Silva, Flávio Santos Lopes, Álissy Fernanda Cardoso Vieira Valois, Cláudia Vieira Prudêncio
The influence of environmental factors on Salmonella sensitivity to nisin in vitro and in refrigerated orange juice were investigated. Nisin activity was observed in the different conditions, but the highest efficiency was achieved at lower pH (4.0) and with higher bacteriocin concentration (174 µM). Moreover, the bactericidal action was directly proportional to the incubation period. When tested in
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Comparison of PetrifilmTM AC and Pour plate techniques used for the heterotrophic aerobic bacterial count in water FEMS Microbiol. Lett. (IF 2.2) Pub Date : 2024-04-30 Faith Mkhwanazi, Tshilidzi Mazibuko, Olivia Mosoma, Malefaso Rathebe, Mrudula Patel
Heterotrophic bacteria (HPC) are commonly found in water samples. While these bacterial counts do not necessarily indicate a health hazard, high counts provide a good indication of the efficiency of water disinfection and integrity of distribution systems. The aim of this study was to compare the PetrifimTM AC method to the Pour Plate technique for the testing of HPC in water samples. Artificially
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Deletion of yghZ in Escherichia coli promotes growth in presence of furfural with xylose as carbon source FEMS Microbiol. Lett. (IF 2.2) Pub Date : 2024-04-26 Bilal Jilani
Thermo-acidic pretreatment of lignocellulosic biomass is required to make it amenable to microbial metabolism and results in generation of furfural due to breakdown of pentose sugars. Furfural is toxic to microbial metabolism and results in reduced microbial productivity and increased production costs. This study asks if deletion of yghZ gene which encodes a NADPH-dependent aldehyde reductase enzyme
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Identification and characterization of a novel type of ketohexokinase from the haloarchaeon Haloferax volcanii FEMS Microbiol. Lett. (IF 2.2) Pub Date : 2024-04-08 Marius Ortjohann, Peter Schönheit
Ketohexokinase (KHK) catalyzes the ATP-dependent phosphorylation of fructose, forming fructose-1-phosphate and ADP. The enzyme is well studied in Eukarya, in particular in human and other vertebrates, but homologs have not been identified in Bacteria and Archaea. Here we report the identification of a novel type of KHK from the haloarchaeon Haloferax volcanii (HvKHK). The encoding gene khk was identified
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Diagnostic accuracy of the IFN-γ release assay using RD1 immunodominant T-cell antigens for diagnosis of extrapulmonary tuberculosis FEMS Microbiol. Lett. (IF 2.2) Pub Date : 2024-03-27 Setareh Mamishi, Babak Pourakbari, Reihaneh Hosseinpour Sadeghi, Majid Marjani, Shima Mahmoudi
The diagnosis of extrapulmonary tuberculosis (EPTB) poses a significant challenge, with controversies surrounding the accuracy of IFN-γ release assays (IGRAs). This study aimed to assess the diagnostic accuracy of RD1 immunodominant T-cell antigens, including ESAT-6, CFP-10, PE35, and PPE68 proteins, for immunodiagnosis of EPTB. Twenty-nine patients with EPTB were enrolled, and recombinant PE35, PPE68
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Bacterial diversity in a continuum from supraglacial habitats to a proglacial lake on the Tibetan Plateau FEMS Microbiol. Lett. (IF 2.2) Pub Date : 2024-03-22 Tingting Xing, Keshao Liu, Mukan Ji, Yuying Chen, Yongqin Liu
Mountain glaciers are frequently assessed for their hydrological connectivity from glaciers to proglacial lakes. Ecological process on glacier surfaces and downstream ecosystems have often been investigated separately, but few studies have focused on the connectivity between the different glacial habitats. Therefore, it remains a limited understanding of bacterial community assembly across different
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An extensive ion-pair/hydrogen-bond network contributes to the thermostability of the MutL ATPase domain from Aquifex aeolicus FEMS Microbiol. Lett. (IF 2.2) Pub Date : 2024-03-22 Ayaka Shibuya, Maki Yokote, Atsushi Suzuki, Kenji Fukui, Takato Yano
Proteins from hyperthermophiles often contain a large number of ionic interactions. Close examination of the previously determined crystal structure of the ATPase domain of MutL from a hyperthermophile, Aquifex aeolicus, revealed that the domain contains a continuous ion-pair/hydrogen-bond network consisting of 11 charged amino acid residues on a β-sheet. Mutations were introduced to disrupt the network
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Lactococcus cell envelope proteases enable lactococcal growth in minimal growth media supplemented with high molecular weight proteins of plant and animal origin FEMS Microbiol. Lett. (IF 2.2) Pub Date : 2024-03-12 Lise Friis Christensen, Ida Nynne Laforce, Judith C M Wolkers-Rooijackers, Martin Steen Mortensen, Eddy J Smid, Egon Bech Hansen
Lactic acid bacteria (LAB) have evolved into fastidious microorganisms that require amino acids from environmental sources. Some LAB have cell envelope proteases (CEPs) that drive the proteolysis of high molecular weight proteins like casein in milk. CEP activity is typically studied using casein as the predominant substrate, even though CEPs can hydrolyze other protein sources. Plant protein hydrolysis
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Application and development of a TaqMan-based real-time PCR assay for rapid detection of snakehead vesiculovirus FEMS Microbiol. Lett. (IF 2.2) Pub Date : 2024-03-08 Cuiping Gong, Panpan Zhu, Jiaxin Ye, Jianfeng Lou, Liwen Zhang, Xiaodan Liu, Weiguang Kong
Snakehead vesiculovirus (SHVV) is one of the primary pathogens responsible for viral diseases in the snakehead fish. A TaqMan-based real-time PCR assay was established for the rapid detection and quantification of SHVV in this study. Specific primers and fluorescent probes were designed for phosphoprotein (P) gene, and after optimizing the reaction conditions, the results indicated that the detection
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An experience of multidisciplinary tutorials sessions about antibiotics in the third year of pharmacy studies in Angers, France: Learning assessment and evaluation of students’ feelings by a mixed approach FEMS Microbiol. Lett. (IF 2.2) Pub Date : 2024-03-07 Audrey Taisne, Samuel Legeay, Isabelle Baglin, Olivier Duval, Matthieu Eveillard
For undergraduate pharmacy students, the first step of antimicrobial stewardship learning objectives is to integrate antimicrobial knowledge from the foundational sciences. We hypothesised that using a multidisciplinary approach including two sessions of tutorials could be relevant in term of students’ interest, satisfaction and learning retention time. The evaluation of students’ feelings was based
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Unlocking the plant growth promoting potential of yeast spp.: Exploring species from the Moroccan extremophilic environment for enhanced plant growth and sustainable farming FEMS Microbiol. Lett. (IF 2.2) Pub Date : 2024-02-29 Anas Raklami, Olubukola Oluranti Babalola, Martin Jemo, Ahmed Nafis
In this study, we successfully isolated two distinct yeasts from Moroccan extreme environments. These yeasts were subjected to molecular characterization by analyzing their ITS regions. Our research thoroughly characterizes plant growth-promoting (PGP) abilities and their drought and salt stress tolerance. In a greenhouse assay, we examined the impact of selected yeasts on Medicago sativa’s growth
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A comprehensive diversity analysis on the gut microbiomes of ASD patients: from alpha, beta to gamma diversities FEMS Microbiol. Lett. (IF 2.2) Pub Date : 2024-02-29 Hongju (Daisy) Chen, Lianwei Li, Fubing Yu, Zhanshan (Sam) Ma
Autism spectrum disorder (ASD) is estimated to influence as many as 1% children worldwide, but its etiology is still unclear. It has been suggested that gut microbiomes play an important role in regulating abnormal behaviors associated with ASD. A de facto standard analysis on the microbiome-associated diseases has been diversity analysis, and nevertheless, existing studies on ASD-microbiome relationship
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Variation in growth rates between cultures hinders the cultivation of ammonia-oxidizing bacteria FEMS Microbiol. Lett. (IF 2.2) Pub Date : 2024-02-24 Rino Isshiki, Hirotsugu Fujitani, Satoshi Tsuneda
Ammonia-oxidizing bacteria, key players in the nitrogen cycle, have been the focus of extensive research. Numerous novel species have been isolated and their growth dynamics were studied. Despite these efforts, controlling their growth to obtain diverse physiological findings remains a challenge. These bacteria often fail to grow, even under optimal conditions. This unpredictable growth pattern could
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Enhancement of yellow pigments production via high CaCl2 stress fermentation of Monascus purpureus FEMS Microbiol. Lett. (IF 2.2) Pub Date : 2024-02-19 Yuqing Bai, Wei Zhang, Ruonan Guo, Jiyuan Yu, Yurong Wang
Monascus pigments (MPs) are a kind of natural ingredient fermented by Monascus spp., which contains three types of pigments: red, orange and yellow ones. Monascus yellow pigments have a restricted yield and cannot meet industrial application. The method and mechanism of CaCl2 improving yellow pigments production by liquid fermentation of M. purpureus M8 were studied in order to overcome the low yield
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Methylocystis dominates methane oxidation in glacier foreland soil at elevated temperature FEMS Microbiol. Lett. (IF 2.2) Pub Date : 2024-02-17 Xinshu Zhu, Yongcui Deng, Yongqin Liu
Methane-oxidizing bacteria (methanotrophs) play an important role in mitigating methane emissions in various ecological environments, including cold regions. However, the response of methanotrophs in these cold environments to extreme temperatures above the in-situ temperature has not been thoroughly explored. Therefore, this study collected soil samples from Longxiazailongba (LXZ) and Qiangyong (QY)
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New Genomic techniques applied to food cultures: a powerful contribution to innovative, safe and sustainable food products FEMS Microbiol. Lett. (IF 2.2) Pub Date : 2024-02-07 Fabio Dal Bello, Laetitia Bocquet, Audrey Bru, Svend Laulund, Ronnie Machielsen, Matteo Raneri, Vincent Sewalt, Noël van Peij, Patrice Ville, Federica Volonté, Yolanda White, Jakub Rusek
Non-transgenic New Genomic Techniques (NGTs) have emerged as a promising tool for food industries, allowing food cultures to contribute to an innovative, safe and more sustainable food system. NGTs have the potential to be applied to microorganisms, delivering on challenging performance traits like texture, flavour and an increase of nutritional value. This paper brings insights on how non-transgenic
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Microbes and us: microbiology literacy in Greece FEMS Microbiol. Lett. (IF 2.2) Pub Date : 2024-02-02 Hera Karayanni, Eleni Motsiou, Vasiliki Sapountzi, Lydia Meggou, Maria Pagkoutsou, Aikaterini Triantafyllidi, Alexandra-Kyparisia Markouti, Sevasti Zervou, Stelios Anastasopoulos, George Efthimiou
Microbes are ubiquitous and provide numerous services to humans and our planet. However, a query arises as to whether these microbial services are valued by the general public especially after unprecedented conditions like the COVID-19 pandemic. In this context a survey was conducted to investigate the concept of microbe in Greece. Thematic analysis of 672 anonymous responses (age range 4–75yo) received
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The MAT1 locus is required for microconidia-mediated sexual fertility in the rice blast fungus FEMS Microbiol. Lett. (IF 2.2) Pub Date : 2024-02-02 Kohtetsu Kita, Momotaka Uchida, Tsutomu Arie, Tohru Teraoka, Hisatoshi Kaku, Yasukazu Kanda, Masaki Mori, Takayuki Arazoe, Takashi Kamakura
Rice blast fungus (Pyricularia oryzae) is a heterothallic ascomycete that causes the most destructive disease in cultivated rice worldwide. This fungus reproduces sexually and asexually, and its mating type is determined by the MAT1 locus, MAT1-1 or MAT1-2. Interestingly, most rice-infecting field isolates show a loss of female fertility, but the MAT1 locus is highly conserved in female-sterile isolates
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Merging and concatenation of sequencing reads: a bioinformatics workflow for the comprehensive profiling of microbiome from amplicon data FEMS Microbiol. Lett. (IF 2.2) Pub Date : 2024-02-02 Meganathan P Ramakodi
A comprehensive profiling of microbial diversity is essential to understand the ecosystem functions. Universal primer sets such as the 515Y/926R could amplify a part of 16S and 18S rRNA and infer the diversity of prokaryotes and eukaryotes. However, the analyses of mixed sequencing data pose a bioinformatics challenge; the 16S and 18S rRNA sequences need to be separated first and analysed individually/
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Knockout of adenylosuccinate synthase purA increases susceptibility to colistin in Escherichia coli FEMS Microbiol. Lett. (IF 2.2) Pub Date : 2024-02-02 Tomonori Kano, Kazuya Ishikawa, Kazuyuki Furuta, Chikara Kaito
Colistin is a cationic cyclic antimicrobial peptide used as a last resort against multidrug-resistant gram-negative bacteria. To understand the factors involved in colistin susceptibility, we screened colistin-sensitive mutants from an E. coli gene-knockout library (Keio collection). The knockout of purA, whose product catalyzes the synthesis of adenylosuccinate from IMP in the de novo purine synthesis
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Interleukin-22 (IL-22) Enhanced the Mucosal Barrier and Inhibited the Invasion of Salmonella enterica in Human induced Pluripotent Stem Cell-derived Small Intestinal Epithelial Cells FEMS Microbiol. Lett. (IF 2.2) Pub Date : 2024-01-25 Fuka Yamazaki, Kyosuke Kobayashi, Junko Mochizuki, Toshihiro Sashihara
Human induced pluripotent stem cell-derived small intestinal epithelial cell (hiPSC-SIEC) monolayers are useful in vitro models for evaluating the gut mucosal barrier; however, their reactivity to cytokines, which are closely related to the regulation of mucosal barrier function, remains unclear. Interleukin (IL)-22 is a cytokine that contributes to regulate the mucosal barrier in the intestinal epithelia
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Analysis of CRISPR-Cas loci distribution in Xanthomonas citri and its possible control by the quorum sensing system FEMS Microbiol. Lett. (IF 2.2) Pub Date : 2024-01-20 Paula Maria Moreira Martins, Laís Moreira Granato, Túlio Morgan, Julia Lopes Nalin, Marco Aurélio Takita, Poliane Alfenas-Zerbini, Alessandra Alves de Souza
Xanthomonas is an important genus of plant-associated bacteria that causes significant yield losses of economically important crops worldwide. Different approaches have assessed genetic diversity and evolutionary interrelationships among the Xanthomonas species. However, information from clustered regularly interspaced short palindromic repeats (CRISPR) has yet to be explored. In this work, we analyzed
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Bacteria Involved in the Sulfur Cycle in Tarballs Collected from the Alabama Gulf Coast FEMS Microbiol. Lett. (IF 2.2) Pub Date : 2024-01-17 Joo-Han Gwak, Sung-Keun Rhee, Joong-Wook Park
Tarballs are formed from released or discharged crude oil containing sulfur compounds. A considerable amount and variety of sulfate-reducing bacteria (SRB) and sulfur-oxidizing bacteria (SOB) were identified in tarballs collected from the intertidal and supratidal zones of Alabama's Gulf beaches. Amplicon sequencing of the bacterial 16S rRNA gene showed that SRB were more abundantly distributed in
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Light irradiation changes the regulation pattern of BtCrgA on carotenogenesis in Blakeslea trispora FEMS Microbiol. Lett. (IF 2.2) Pub Date : 2024-01-11 Jiamin Yang, Mingxi Zeng, Hui Wu, Zhenlin Han, Zhiyan (Rock) Du, Xiaobin Yu, Wei Luo
CrgA has been shown to be a negative regulator of carotenogenesis in some filamentous fungi, while light irradiation is an inducible environmental factor for carotenoid biosynthesis. To clarify the relationship between CrgA and light-inducible carotenogenesis in Blakeslea trispora, the cis-acting elements of the btcrgA promoter region were investigated, followed by the analyses of correlation between
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Theoretical hypothesis in a direct electron transfer between non-interacting Fe-S proteins within an artificial fusion FEMS Microbiol. Lett. (IF 2.2) Pub Date : 2024-01-10 Jae Kyu Lim
Reduction of CO2 to formate utilizing formate dehydrogenases has been attempted biologically and electrochemically. However, the conversion efficiency is very low due to the low energy potential of electron donors and/or electron competition with other electron acceptors. To overcome such a low conversion efficiency, I focused on a direct electron transfer between two unrelated redox enzymes for the
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Global transcriptome analysis of Pseudomonas aeruginosa NT06 response to potassium chloride, sodium lactate, sodium citrate, and microaerophilic conditions in a fish ecosystem. FEMS Microbiol. Lett. (IF 2.2) Pub Date : 2024-01-09 Natalia Tomaś,Kamila Myszka,Łukasz Wolko,Wojciech Juzwa
Pseudomonas aeruginosa is an opportunistic pathogen that recently has been increasingly isolated from foods, especially from minimally processed fish-based products. Those are preserved by the addition of sodium chloride (NaCl) and packaging in a modified atmosphere. However, the current trends of minimizing NaCl content may result in an increased occurrence of P. aeruginosa. NaCl can be replaced with
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Methane production from the biodegradation of lignite with different sizes by mixed fungi-methanogen microflora. FEMS Microbiol. Lett. (IF 2.2) Pub Date : 2024-01-09 Longzhen Gao,Xiao Feng,Yixuan Zhang,Hongguang Guo,Xiaogang Mu,Zaixing Huang,Michael Urynowicz
Biogenic coalbed methane (CBM) is a developing clean energy source. However, it is unclear how the mechanisms of bio-methane production with different sizes of coal. In this work, pulverized coal (PC) and lump coal (LC) were used for methane production by mixed fungi-methanogen microflora. The lower methane production from LC was observed. The aromatic carbon of coal was degraded slightly by 2.17%