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Prof. Armen Trchounian (1956–2020) FEMS Microbiol. Lett. (IF 1.987) Pub Date : 2020-12-22 Schink B.
Prof. Armen Trchounian, Head of the Department of Biochemistry, Microbiology, and Biotechnology at Yerevan State University (Yerevan, Armenia), passed away on 18 November 2020, aged 64 years, in the context of a COVID-19 infection. With him, the FEMS community loses a highly competent scientist and an always constructive and friendly ambassador of his beloved home country Armenia.
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Proof of the triple prerequisite conditions which are essential for carbapenem resistance development in Klebsiella pneumoniae by using Radiation-mediated mutagenesis FEMS Microbiol. Lett. (IF 1.987) Pub Date : 2021-01-19 Naina Adren Pinto; Roshan D'Souza; Le Phuong Nguyen; Thao Nguyen Vu; Dongeun Yong
Evolution of multi-drug resistant bacteria has led to worldwide research to better understand the various resistance mechanisms in these strains. Every year, novel information on carbapenem resistance and its mechanisms is being discovered. In this study, radiation-mediated mutagenesis was used to transform a carbapenem-resistant Klebsiella pneumoniae strain to a carbapenem-susceptible bacterium. Through
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Simultaneous detection of Streptococcus Pneumoniae and prevention of carryover contamination using multiple cross displacement amplification with Antarctic thermal sensitive uracil-DNA-glycosylase and a lateral flow biosensor FEMS Microbiol. Lett. (IF 1.987) Pub Date : 2021-01-19 Linlin Yan; Fan Zhao; Lina Niu; Yu Cai; Lei Wu; Xiaoxue Zhu; Jinqing Nong; Shoukui Hu
Streptococcus pneumoniae is an important clinical pathogenic bacterium that is the primary cause of meningitis, septicemia, and community-acquired pneumonia. The mortality rate of pneumococcal disease is high, especially in children younger than 5 years old. Rapid and accurate detection of S. pneumoniae is critical for clinical diagnosis. A ply gene-based multiple cross displacement amplification (MCDA)
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Functional characterization of the putative FAD synthase from Mycoplasma hyopneumoniae FEMS Microbiol. Lett. (IF 1.987) Pub Date : 2021-01-16 Amanda Malvessi Cattani; Camila Vieira Pinheiro; Irene Silveira Schrank; Franciele Maboni Siqueira
In bacteria, the biosynthesis of the cofactor flavin adenine dinucleotide (FAD), important in many physiological responses, is catalyzed by the bifunctional enzyme FAD synthase (FADSyn) which converts riboflavin into FAD by both kinase and adenylylation activity. The in silico 3D structure of a putative FADSyn from Mycoplasma hyopneumoniae (MhpFADSyn), the etiological agent of enzootic pneumonia was
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Issues in cyanobacterial taxonomy: comprehensive case study of unbranched, false branched and true branched heterocytous cyanobacteria FEMS Microbiol. Lett. (IF 1.987) Pub Date : 2021-01-16 Deeksha Mishra; Aniket Saraf; Naresh Kumar; Sagarika Pal; Prashant Singh
The order Nostocales is represented by morphologically diverse forms with respect to the branching patterns and polarity of the filaments. With growing understanding of taxonomy and systematics, members of the order Nostocales have also undergone multiple taxonomic revisions. The last decade has seen a surge in the description of new genera and families within the order Nostocales. In this study, we
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Immunoglobulin-fold containing bacterial adhesins: Molecular and structural perspectives in host tissue colonization and infection FEMS Microbiol. Lett. (IF 1.987) Pub Date : 2020-12-23 Shruti Chatterjee; Aditya J Basak; Asha V Nair; Kheerthana Duraivelan; Dibyendu Samanta
Immunoglobulin (Ig) domains are one of the most widespread protein domains encoded by the human genome and are present in a large array of proteins with diverse biological functions. These Ig domains possess a central structure, the immunoglobulin fold, which is a sandwich of two β sheets, each made up of anti-parallel β strands, surrounding a central hydrophobic core. Apart from humans, proteins containing
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Elimination of GlnKAmtB affects serine biosynthesis and improves growth and stress tolerance of Escherichia coli under nutrient-rich conditions FEMS Microbiol. Lett. (IF 1.987) Pub Date : 2020-11-26 Romina Frare; Margarita Stritzler; Cecilia Pascuan; Karen Liebrenz; Luisa Galindo-Sotomonte; Gabriela Soto; Pablo Iván Nikel; Nicolás Ayub
Nitrogen is a most important nutrient resource for Escherichia coli and other bacteria that harbor the glnKamtB operon, a high-affinity ammonium uptake system highly interconnected with cellular metabolism. Although this system confers an advantage to bacteria when growing under nitrogen-limiting conditions, little is known about the impact of these genes on microbial fitness under nutrient-rich conditions
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Production of methylmercury by methanogens in mercury contaminated estuarine sediments FEMS Microbiol. Lett. (IF 1.987) Pub Date : 2020-11-26 Yuwei Wang; Spencer Roth; Jeffra K Schaefer; John R Reinfelder; Nathan Yee
Anaerobic bacteria are known to produce neurotoxic methylmercury [MeHg] when elemental mercury [Hg(0)] is provided as the sole mercury source. In this study, we examined the formation of MeHg in anaerobic incubations of sediment collected from the San Jacinto River estuary (Texas, USA) amended with aqueous Hg(0) to investigate the microbial communities involved in the conversion of Hg(0) to MeHg. The
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Analysis of disulphide bond linkage between CoA and protein cysteine thiols during sporulation and in spores of Bacillus species FEMS Microbiol. Lett. (IF 1.987) Pub Date : 2020-11-18 Alexander Zhyvoloup; Bess Yi Kun Yu; Jovana Baković; Mathew Davis-Lunn; Maria-Armineh Tossounian; Naam Thomas; Yugo Tsuchiya; Sew Yeu Peak-Chew; Sivaramesh Wigneshweraraj; Valeriy Filonenko; Mark Skehel; Peter Setlow; Ivan Gout
Spores of Bacillus species have novel properties, which allow them to lie dormant for years and then germinate under favourable conditions. In the current work, the role of a key metabolic integrator, coenzyme A (CoA), in redox regulation of growing cells and during spore formation in Bacillus megaterium and Bacillus subtilis is studied. Exposing these growing cells to oxidising agents or carbon deprivation
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Video-based learning to enhance teaching of practical microbiology FEMS Microbiol. Lett. (IF 1.987) Pub Date : 2020-12-22 Katrina Lacey; J Gerard Wall
Video-based learning is an increasingly important methodology in higher education and has particular value in practical teaching. In order to enhance learning and promote student engagement in our undergraduate microbiology programme, we designed and produced a suite of teaching videos which demonstrate laboratory techniques core to the syllabus. The methods were demonstrated by PhD students and the
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Corrigendum to: Microbial products from wastes and residues FEMS Microbiol. Lett. (IF 1.987) Pub Date : 2020-11-25
In the originally published version of this manuscript, the following text and reference Acknowledgements were inadvertently omitted:
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Transcriptional analysis of metabolic and virulence genes associated with biofilm formation in Piscirickettsia salmonis strains FEMS Microbiol. Lett. (IF 1.987) Pub Date : 2020-10-31 A Zúñiga; C Solis; C Cartes; G Nourdin; A Yañez; A Romero; D Haussmann; J Figueroa
Piscirickettsia salmonis is a facultative intracellular bacterium that generates piscirickettsiosis affecting salmonids in Chile. The bacterium has the adaptability to survive in the marine environment under multiple stressful conditions. In this sense, this work focused on the analysis of a gene battery associated with biofilm formation under different culture conditions and on the adaptability of
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Cadmium transport activity of four mercury transporters (MerC, MerE, MerF and MerT) and effects of the periplasmic mercury-binding protein MerP on Mer-dependent cadmium uptake FEMS Microbiol. Lett. (IF 1.987) Pub Date : 2020-10-29 Yuka Ohshiro; Shimpei Uraguchi; Ryosuke Nakamura; Yasukazu Takanezawa; Masako Kiyono
Mercury superfamily proteins, i.e. inner membrane-spanning proteins (MerC, MerE, MerF and MerT) and a periplasmic mercury-binding protein (MerP), transport mercury into the cytoplasm. A previous study demonstrated that a Mer transporter homolog exhibits cadmium transport activity; based on this, the present study aimed to evaluate the cadmium transport activity of MerC, MerE, MerF and MerT and the
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Development and application of a real-time loop-mediated isothermal amplification method for quantification of Acetobacter aceti in red wine FEMS Microbiol. Lett. (IF 1.987) Pub Date : 2020-10-06 Jingfeng Zhang; Li Wang; Lei Shi; Xun Chen; Meidan Liang; Lichao Zhao
This study reports the development and optimization of a real-time loop-mediated isothermal amplification (qLAMP) method for rapid detection of Acetobacter aceti strain in red wine samples. Our results showed that the primers and probes designed for 16S rRNA were effective for A. aceti detection. The quantification limit of real-time polymerase chain reaction (qPCR) and qLAMP in pure culture was 2
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Microbial products from wastes and residues FEMS Microbiol. Lett. (IF 1.987) Pub Date : 2020-10-16 Seraphim Papanikolaou; George Aggelis
Our society currently faces the twin challenges of resource depletion and waste accumulation leading to rapidly escalating raw material costs and increasingly expensive and restrictive waste disposal issue. A plethora of agricultural, aquaculture, food-processing and industrial activities generates enormous quantities of organic wastes and residues every year, the eco-friendly treatment of which constitutes
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Gut microbiota composition in obese and non-obese adult relatives from the highlands of Papua New Guinea FEMS Microbiol. Lett. (IF 1.987) Pub Date : 2020-10-06 Marinjho E Jonduo; Lorry Wawae; Geraldine Masiria; Wataru Suda; Masahira Hattori; Lena Takayasu; Mohammad Y Abdad; Andrew R Greenhill; Paul F Horwood; William Pomat; Masahiro Umezaki
Obesity is a condition that results from an imbalance between energy intake and expenditure. Recently, obesity has been linked to differences in the composition of gut microbiota. To examine this association in Papua New Guinea (PNG) highlanders, fecal samples were collected from 18 adults; nine obese participants were paired with their non-obese relative. Amplification of the 16S rRNA gene targeting
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Assessment of phage-mediated inhibition of Salmonella Typhimurium treated with sublethal concentrations of ceftriaxone and ciprofloxacin FEMS Microbiol. Lett. (IF 1.987) Pub Date : 2020-10-05 Gibeom Jeon; Juhee Ahn
This study was designed to evaluate the synergistic effect of phage (P22) and antibiotic on the inhibition of Salmonella Typhimurium exposed to ceftriaxone (CEF) and ciprofloxacin (CIP). The effect of phage and antibiotic treatments was evaluated by plaque size, disk diffusion, antibiotic susceptibility and phage multiplication assays. The sequential treatment effect of phage and antibiotic was carried
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Temperature-sensitive recombinant subtilisin protease variants that efficiently degrade molecular biology enzymes FEMS Microbiol. Lett. (IF 1.987) Pub Date : 2020-10-05 Vanessa C Thompson; Bailey E McGuire; Mia S Frier; Max S G Legg; Tyler W Dyer; Geoff Gudavicius; Sheila Potter; Francis E Nano
We used error-prone PCR to generate mutations in a subtilisin protease-encoding gene, and screened for recombinants that expressed temperature-sensitive (TS) variants. From the dozens of mutations that we detected in the recombinant genes we found that those mutations that affected aspartate-75 had the most profound effect on temperature stability. We thus focused our analysis on two variants of subtilisin
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Aerobic microbial communities in the sediments of a marine oxygen minimum zone FEMS Microbiol. Lett. (IF 1.987) Pub Date : 2020-09-25 Sabyasachi Bhattacharya; Chayan Roy; Subhrangshu Mandal; Jagannath Sarkar; Moidu Jameela Rameez; Nibendu Mondal; Tarunendu Mapder; Sumit Chatterjee; Prosenjit Pyne; Masrure Alam; Prabir Kumar Haldar; Rimi Roy; Svetlana Fernandes; Aditya Peketi; Ranadhir Chakraborty; Aninda Mazumdar; Wriddhiman Ghosh
The ecology of aerobic microorganisms is never explored in marine oxygen minimum zone (OMZ) sediments. Here we reveal aerobic bacterial communities along ∼3 m sediment-horizons of the eastern Arabian Sea OMZ. Sulfide-containing sediment-cores retrieved from 530 mbsl (meters beneath the sea-level) and 580 mbsl were explored at 15–30 cm intervals, using metagenomics, pure-culture-isolation, genomics
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The type of microorganism and substrate determines the odor fingerprint of dried bacteria targeting microbial protein production FEMS Microbiol. Lett. (IF 1.987) Pub Date : 2020-09-24 Myrsini Sakarika; Daylan Amelia Tzompa Sosa; Mathilde Depoortere; Hayley Rottiers; Ramon Ganigué; Koen Dewettinck; Korneel Rabaey
The rapidly increasing demand for protein has led to the pursuit of new protein sources, among which microbial protein (MP) is one of the most promising. Although the nutritional properties of MP are important and often well-studied, the sensory properties of the microbial cells will in part determine the commercial success of the product and are much less investigated. Here we assessed the odor fingerprint
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Strengthening the TCA cycle to alleviate metabolic stress due to blocking by-products synthesis pathway in Klebsiella pneumoniae. FEMS Microbiol. Lett. (IF 1.987) Pub Date : 2020-09-09 Mengmeng Xie,Xinyao Lu,Hong Zong,Bin Zhuge
1,3-Propanediol (1,3-PDO) is an important synthetic monomer for the production of polytrimethylene terephthalate (PTT). Here, we engineered Klebsiella pneumoniae by a multi-strategy to improve 1,3-PDO production and reduce by-products synthesis. First, the 2,3-butanediol (2,3-BDO) synthesis pathway was blocked by deleting the budB gene, resulting in a 74% decrease of 2,3-BDO titer. The synthesis of
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Impact of inter-amoebic phagocytosis on the L. pneumophila growth. FEMS Microbiol. Lett. (IF 1.987) Pub Date : 2020-08-29 Rafik Dey,Mouh Rayane Mameri,Selena Trajkovic-Bodennec,Jacques Bodennec,Pierre Pernin
Free-living amoebae are known to act as replication niches for the pathogenic bacterium Legionella pneumophila in freshwater environments. However, we previously reported that some strains of the Willaertia magna species are more resistant to L. pneumophila infection and differ in their ability to support its growth. From this observation, we hypothesize that L. pneumophila growth in environment could
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Tyrosine tRNA synthetase as a novel extracellular immunomodulatory protein in Streptococcus anginosus. FEMS Microbiol. Lett. (IF 1.987) Pub Date : 2020-09-14 Yu Shimoyama,Taichi Ishikawa,Yoshitoyo Kodama,Shigenobu Kimura,Minoru Sasaki
Streptococcus anginosus is frequently detected in patients with infective endocarditis, abscesses or oral cancer. Although S. anginosus is considered the causative pathogen of these diseases, the pathogenic mechanisms of the bacterium have remained unclear. Previously, we suggested that an extracellular antigen from S. anginosus (SAA) serves as a pathogenic factor by inducing nitric oxide production
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Degradation of butachlor and propanil by Pseudomonas sp. strain But2 and Acinetobacter baumannii strain DT. FEMS Microbiol. Lett. (IF 1.987) Pub Date : 2020-09-08 Ha Danh Duc,Nguyen Thi Dieu Thuy,Huynh Thi Thanh Truc,Nguyen Thi Huynh Nhu,Nguyen Thi Oanh
Herbicides have been extensively used globally, resulting in severe environmental pollution. Novel butachlor-degrading Pseudomonas sp. strain But2 isolated from soil can degrade butachlor regardless of the concentration and grows without a lag phase. Specific degradation was increased at 0.01–0.1 mM, and did not change significantly at higher concentrations. During degradation, 2-chloro-N-(2,6-diethylphenyl)
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Unraveling the xylanolytic potential of Acidobacteria bacterium AB60 from Cerrado soils. FEMS Microbiol. Lett. (IF 1.987) Pub Date : 2020-09-08 Gisele Regina Rodrigues,Otávio Henrique Bezerra Pinto,Luís Felipe Schroeder,Gabriel da Rocha Fernandes,Ohana Yonara Assis Costa,Betania Ferraz Quirino,Eiko Eurya Kuramae,Cristine Chaves Barreto
The presence of genes for glycosyl hydrolases in many Acidobacteria genomes indicates an important role in the degradation of plant cell wall material. Acidobacteria bacterium AB60 was obtained from Cerrado oligotrophic soil in Brazil, where this phylum is abundant. The 16S rRNA gene analyses showed that AB60 was closely related to the genera Occallatibacter and Telmatobacter. However, AB60 grew on
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Comparison of microbial diversity during two different wine fermentation processes. FEMS Microbiol. Lett. (IF 1.987) Pub Date : 2020-09-08 Miroslav Böhmer,Dávid Smoľak,Katarína Ženišová,Zuzana Čaplová,Domenico Pangallo,Andrea Puškárová,Mária Bučková,Tereza Cabicarová,Jaroslav Budiš,Katarína Šoltýs,Diana Rusňáková,Tomáš Kuchta,Tomáš Szemes
Wine production is a complex procedure in which an important role is played by many microorganisms, particularly yeasts and bacteria. In modern wineries, alcoholic fermentation is usually carried out by adding microbial starter cultures of Saccharomyces cerevisiae strains for precisely controlled production. Nowadays, in the Slovak Republic, autochthonous vinification is getting more popular. The present
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A comparison of the inhibitory activities of Lactobacillus and Bifidobacterium against Penicillium expansum and an analysis of potential antifungal metabolites. FEMS Microbiol. Lett. (IF 1.987) Pub Date : 2020-08-26 Nanzhen Qiao,Leilei Yu,Chengcheng Zhang,Chaozhi Wei,Jianxin Zhao,Hao Zhang,Fengwei Tian,Qixiao Zhai,Wei Chen
The infection of fruits by Penicillium expansum (P. expansum) do not only cause economic loss but also potentially endanger human health, especially because few biocontrol agents against this fungus have been well studied yet. In this work, to verity the antifungal activity against P. expansum of 22 Bifidobacterium and 44 Lactobacillus, dual-culture overlay assay, microtiter plate well assay and agar
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A MarR-family transcriptional factor MapR positively regulates actinorhodin production in Streptomyces coelicolor. FEMS Microbiol. Lett. (IF 1.987) Pub Date : 2020-08-21 Zhong Xu,Yuanyuan Li
Production of secondary metabolites is tightly regulated by transcriptional regulators in Streptomyces. Many regulators have been identified by gene disruption in Streptomyces; however, there are still unknown regulatory genes yet to be revealed due to gene silence under laboratory culture conditions. In present study, a T7 expression system was used to identify novel regulators by gene deletion and
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Transition-state theory-based analysis of diffusion of water in yeast cells FEMS Microbiol. Lett. (IF 1.987) Pub Date : 2020-09-21 Mehvish K Durrani; Jonghoon Kang
ABSTRACTScientific journals have played an essential role in the diffusion of research breakthroughs. For many years there was no competition between journals, but, in recent decades they have become categorized by a careful assessment of their published contents based on several metric parameters. Of greater note, the ‘prestige index’ has become an essential tool used by public and private institutions
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Trends in Microbiology publications: are classic scientific journals condemned to extinction? FEMS Microbiol. Lett. (IF 1.987) Pub Date : 2020-08-29 Sergi Maicas,Ruth Sánchez-Fresneda,Juan-Carlos Argüelles
Scientific journals have played an essential role in the diffusion of research breakthroughs. For many years there was no competition between journals, but, in recent decades they have become categorized by a careful assessment of their published contents based on several metric parameters. Of greater note, the ‘prestige index’ has become an essential tool used by public and private institutions to
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Rehydration of dry corn preserves the desirable bacterial community during ensiling. FEMS Microbiol. Lett. (IF 1.987) Pub Date : 2020-08-25 Paula de Almeida Carvalho-Estrada,Pedro Avelino Maia de Andrade,Solidete de Fátima Paziani,Luiz Gustavo Nussio,Maria Carolina Quecine
This study evaluated the rehydration approach of mature corn grains as an alternative for high-moisture corn grain silage production in distinct corn hybrids, storage period, cultivation locations and kernel maturity at plant harvest. High-moisture corn was used as a control. The dry matter content and pH of the silage were measured, and the bacterial community associated with corn grains pre- and
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A novel sophorolipid-producing Candida keroseneae GBME-IAUF-2 as a potential agent in microbial enhanced oil recovery (MEOR). FEMS Microbiol. Lett. (IF 1.987) Pub Date : 2020-08-24 Zahra Ganji,Keivan Beheshti-Maal,Ahmadreza Massah,Zarrindokht Emami-Karvani
The biosurfactants have extensive applications in food and petroleum microbiology. The aims of this research were isolation and characterization of thermo-tolerant biosurfactants from highly producing yeast strains. The Bushnell Hass medium was used for screening the biosurfactant-producing yeasts. Biosurfactant presence was evaluated using oil displacement assay and surface tension test. The best
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Diatom DNA barcodes for forensic discrimination of drowning incidents. FEMS Microbiol. Lett. (IF 1.987) Pub Date : 2020-08-24 Mengyan Liu,Yi Zhao,Yuzhe Sun,Ping Wu,Shiliang Zhou,Liang Ren
The presence of diatoms in victim's internal organs has been regarded as a gold biological evidence of drowning. The idea becomes true at the advent of DNA metabarcoding. Unfortunately, the DNA barcode of diatoms are far from being applicable due to neither consensus on the barcode and nor reliable reference library.In this study we tested 23 pairs of primers, including two new primer pairs, Baci18S
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Bacteriophytochrome from Magnetospirillum magneticum affects phototactic behavior in response to light. FEMS Microbiol. Lett. (IF 1.987) Pub Date : 2020-08-21 Haitao Chen,Dandan Li,Yao Cai,Long-Fei Wu,Tao Song
Phytochromes are a class of photoreceptors found in plants and in some fungi, cyanobacteria, and photoautotrophic and heterotrophic bacteria. Although phytochromes have been structurally characterized in some bacteria, their biological and ecological roles in magnetotactic bacteria remain unexplored. Here, we describe the biochemical characterization of recombinant bacteriophytochrome (BphP) from magnetotactic
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Analysis of 5'-NAD capping of mRNAs in dormant spores of Bacillus subtilis. FEMS Microbiol. Lett. (IF 1.987) Pub Date : 2020-08-21 D Levi Craft,George Korza,Yaqing Zhang,Jens Frindert,Andres Jäschke,Melissa J Caimano,Peter Setlow
Spores of Gram-positive bacteria contain 10s–1000s of different mRNAs. However, Bacillus subtilis spores contain only ∼ 50 mRNAs at > 1 molecule/spore, almost all transcribed only in the developing spore and encoding spore proteins. However, some spore mRNAs could be stabilized to ensure they are intact in dormant spores, perhaps to direct synthesis of proteins essential for spores’ conversion to a
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Kinetic and substrate complex characterization of RamA, a corrinoid protein reductive activase from Methanosarcina barkeri. FEMS Microbiol. Lett. (IF 1.987) Pub Date : 2020-08-25 Katherine A Huening,Ruisheng Jiang,Joseph A Krzycki
In microbial corrinoid-dependent methyltransferase systems, adventitious Co(I)-corrinoid oxidation halts catalysis and necessitates repair by ATP-dependent reductive activases. RamA, an activase with a C-terminal ferredoxin domain with two [4Fe-4S] clusters from methanogenic archaea has been far less studied than the bacterial activases bearing an N-terminal ferredoxin domain with one [2Fe-2S] cluster
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Gut microbiome adaptation to extreme cold winter in wild plateau pika (Ochotona curzoniae) on the Qinghai-Tibet Plateau. FEMS Microbiol. Lett. (IF 1.987) Pub Date : 2020-08-25 Yijie Wang,Rui Zhou,Qiaoling Yu,Tianshu Feng,Huan Li
The Qinghai-Tibet Plateau (QTP) is harsh environment characterized by low temperature, high altitude and hypoxia, while some native mammals may well adapt to the extreme climate. However, how animal gut microbial community structure and function adapt to extreme cold climate is not well understood. Plateau pika (Ochotona curzoniae) is an ideal animal model to study the effects of climate change on
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A toddler SHIME® model to study microbiota of young children. FEMS Microbiol. Lett. (IF 1.987) Pub Date : 2020-08-25 Pauline Bondue,Sarah Lebrun,Bernard Taminiau,Nadia Everaert,Gisele LaPointe,Sebastien Crevecoeur,Georges Daube,Veronique Delcenserie
The ‘first 1,000 days of life’ determine the gut microbiota composition and can have long-term health consequences. In this study, the simulator of the human intestinal microbial ecosystem (SHIME®) model, which represents the main functional sections of the digestive tract, was chosen to study the microbiota of young children. The aim of this study was to reproduce the digestive process of toddlers
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'Bacterial Programmed Cell Death': cellular altruism or genetic selfism? FEMS Microbiol. Lett. (IF 1.987) Pub Date : 2020-08-21 Bhaskar Chandra Mohan Ramisetty,Pavithra Anantharaman Sudhakari
Cell-dependent propagation of the ‘self’ is the driver of all species, organisms, and even genes. Conceivably, elimination of these entities is caused by cellular death. Then, how can genes that cause the death of the same cell evolve? Programmed cell death (PCD) is the gene-dependent self-inflicted death. In multicellular organisms, PCD of a cell confers fitness to the surviving rest of the organism
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The hydrogen threshold of obligately methyl-reducing methanogens. FEMS Microbiol. Lett. (IF 1.987) Pub Date : 2020-08-21 Christopher Feldewert,Kristina Lang,Andreas Brune
Methanogenesis is the final step in the anaerobic degradation of organic matter. The most important substrates of methanogens are hydrogen plus carbon dioxide and acetate, but also the use of methanol, methylated amines, and aromatic methoxy groups appears to be more widespread than originally thought. Except for most members of the family Methanosarcinaceae, all methylotrophic methanogens require
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Recovery of chromium, copper and vanadium combined with electricity generation in two-chambered microbial fuel cells. FEMS Microbiol. Lett. (IF 1.987) Pub Date : 2020-08-05 Kartik S Aiyer
Microbial fuel cells (MFCs) offer a promising solution towards recovery and treatment of heavy metal pollutants. In this study, two-chambered MFCs were employed for recovery of chromium, copper and vanadium (Cr (VI), Cu (II) and V (V)). One g/L concentrations of K2Cr2O7, CuCl2 and NaVO3 served as catholytes, while a mixed culture was used as anolyte. Cr (VI), Cu (II) and V (V) were reduced biologically
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Isolation and plasmid characterisation of Salmonella enterica serovar Albany harbouring mcr-5 from retail chicken meat in Japan. FEMS Microbiol. Lett. (IF 1.987) Pub Date : 2020-08-05 Yuki Wakabayashi,Tsuyoshi Sekizuka,Takahiro Yamaguchi,Akira Fukuda,Masato Suzuki,Ryuji Kawahara,Masumi Taguchi,Makoto Kuroda,Keiko Semba,Hiroto Shinomiya,Kentaro Kawatsu
The emergence of plasmid-mediated colistin resistance genes (mcr), which is occurring in numerous countries, is a worldwide concern, primarily because colistin is a last-resort antibiotic. Compared to E. coli, prevalence of mcr genes in Salmonella is unclear in Japan. Here we screened for mcr-1–5 genes in our collection of Salmonella strains isolated from retail meat products collected in Japan from
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Direct antibacterial and antibiotic resistance modulatory activity of chalcones synthesized from the natural product 2-hydroxy-3,4,6-trimethoxyacetophenone. FEMS Microbiol. Lett. (IF 1.987) Pub Date : 2020-08-05 Thiago Sampaio de Freitas,Jayze da Cunha Xavier,Raimundo L S Pereira,Janaina E Rocha,Dédora F Muniz,Priscila T da Silva,João P da Hora,Hélcio S Dos Santos,Paulo N Bandeira,Carlos E S Nogueira,Alexandre M R Teixeira,Henrique D M Coutinho
Antibiotic for clinical use lose its effectiveness over time due to bacterial resistance. In this work, four chalcones with modifications in their ligands were synthesized from the natural product 2-hydroxy-3,4,6-trimethoxyacetophenone, characterized by nuclear magnetic resonance (NMR) and infrared spectroscopy, and tested in bacterial models to investigate the direct and modifiers effects of the antibiotic
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Enterococcus faecalis and Vibrio harveyicolonize low-density polyethylene and biodegradable plastics under marine conditions. FEMS Microbiol. Lett. (IF 1.987) Pub Date : 2020-07-27 Ilef Hchaichi,Francesca Bandini,Giulia Spini,Mohamed Banni,Pier Sandro Cocconcelli,Edoardo Puglisi
Conventional plastics and bioplastics are known to accumulate in aquatic ecosystems, emerging as new surfaces for biofilm formation and gene exchanges. On the other hand, the fate of non-conventional bioplastics in the marine environment is still unclear. In this study we have measured, by means of crystal violet test and scanning electron microscopy (SEM), the ability of two model bacteria, Vibrio
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The biosurfactants iturin, lichenysin and surfactin, from vaginally isolated lactobacilli, prevent biofilm formation by pathogenic Candida. FEMS Microbiol. Lett. (IF 1.987) Pub Date : 2020-07-25 Jakline Nelson,Ahmed O El-Gendy,Moselhy S Mansy,Mohamed A Ramadan,Ramy K Aziz
Lactic acid bacteria (LAB), particularly lactobacilli, are major components of the vaginal microbiota. Lactobacilli are facultative anaerobes forming a critical line of defense against pathogenic microorganisms, including those forming biofilms, such as Candida spp. This study aimed to investigate the anti-adhesion capabilities of vaginal Lactobacillus isolates against biofilms formed by pathogenic
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Description of Shewanella salipaludis sp. nov., isolated from a salt marsh. FEMS Microbiol. Lett. (IF 1.987) Pub Date : 2020-07-18 Sooyeon Park,Siyu Chen,Jung-Sook Lee,Wonyong Kim,Jung-Hoon Yoon
A Gram-stain-negative and flagellated bacterial strain, SHSM-M6T, was isolated from salt marsh from Yellow Sea, Republic of Korea. Neighbor-joining phylogenetic tree of 16S rRNA gene sequences showed that strain SHSM-M6T belongs to the genus Shewanella. 16S rRNA gene sequence similarity values between strain SHSM-M6T and the type strains of Shewanella species were <98.0%. The average nucleotide identity
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Assessment of the microbiological quality of natural mineral waters according to the manufacturing time of 20 L returnable packs in Brazil. FEMS Microbiol. Lett. (IF 1.987) Pub Date : 2020-07-17 Isabelle da Silva Luz,Luiza Vasconcellos,Valéria de Mello Medeiros,Catia Aparecida Chaia Miranda,Carla de Oliveira Rosas,Márcia Maria Araújo Pimenta,Fernando César Ferreira,Célia Maria Carvalho Pereira Araújo Romão,Marcelo Luiz Lima Brandão,Marize Pereira Miagostovich
This study aimed to assess the microbiological quality of natural mineral waters commercialized in 20 L returnable packs in Brazil by investigating the presence of bacteria and viruses in packs with different manufacturing times (Tm). With this purpose, 99 water samples from 33 lots (n = 3/batch) of 15 brands, obtained from packs with three intervals of Tm, were analyzed. Total coliforms (16.2%), Pseudomonas
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The recruitment of bacterial communities by the plant root system changed by acid mine drainage pollution in soils. FEMS Microbiol. Lett. (IF 1.987) Pub Date : 2020-07-16 Yang Li,Liang Yuan,Sheng Xue,Bingjun Liu,Gang Jin
This study aims to better understand the relationship between the response to acid mine drainage (AMD) stress of tolerant plants and changes in root-related bacterial communities. In this study, reed stems were planted in AMD-polluted and unpolluted soils, and high-throughput sequencing was conducted to analyze the bacterial community composition in the soil, rhizosphere, rhizoplane and endosphere
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The HlyD-like membrane fusion protein All5304 is essential for acid stress survival of the filamentous cyanobacterium Anabaena sp. PCC 7120. FEMS Microbiol. Lett. (IF 1.987) Pub Date : 2020-06-27 Dmitry Shvarev,Iris Maldener
Acid stress is an environmental problem for plants and fresh water cyanobacteria like the filamentous, heterocyst forming species Anabaena sp. PCC 7120 (hereafter Anabaena sp.). Heterocyst differentiation, cell–cell communication and nitrogen fixation has been deeply studied in this model organism, but little is known about the cellular response of Anabaena sp. to decreased pH values, causing acid
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Prevalence, antibiotic susceptibility and characterization of Vibrio parahaemolyticus isolates in China. FEMS Microbiol. Lett. (IF 1.987) Pub Date : 2020-08-08 Tengfei Xie,Quan Yu,Xiong Tang,Jing Zhao,Xujun He
Vibrio parahaemolyticus is a marine and estuarine bacterium that poses a major threat to human health worldwide. In this study, from 2017 to 2019, we evaluated 900 food samples collected from China in 2017, with an aimed to determine the incidence and features of V.parahaemolyticus in ready-to-eat (RTE) foods, shrimp and fish in China. The contamination rates in these were 3.67%, 19.33%, and 10.67%
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Mediterranean Sea bacteria as a potential source of long-chain polyunsaturated fatty acids. FEMS Microbiol. Lett. (IF 1.987) Pub Date : 2020-08-07 Roberta Romano,Noura Raddadi,Fabio Fava
Long-chain polyunsaturated fatty acids (LC-PUFAs), including EPA (eicosapentaenoic acid) and DHA (docosahexaenoic acid) are important nutritional ingredients in fish feed. So far, fish oil has been used as a main source of LC-PUFAs; however, the limited global supply of fish oil is not able to meet the demand of the growing aquaculture sector. Hence, sustainability of aquaculture industry could be
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Shotgun metagenomics of indigenous bacteria collected from the banks of the San Jacinto River for biodegradation of aromatic waste. FEMS Microbiol. Lett. (IF 1.987) Pub Date : 2020-08-06 Rupa Iyer,Ashish Damania
Several Eastern Harris County communities lie near the now enclosed San Jacinto River (SJR) Waste Pit Superfund, a dumping ground for chlorinated dioxins and other paper mill waste products. Currently, no active monitoring of the SJR is conducted for these toxins with the exact concentration and health impact to the area unknown. As such, remediation and monitoring efforts outside of the Superfund
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Adopt a Bacterium: a professional development opportunity for teacher assistants. FEMS Microbiol. Lett. (IF 1.987) Pub Date : 2020-08-05 N P Taschner,L G de Almeida,R A Pose,R C C Ferreira
Science teaching in most Brazilian Universities tends to focus mainly on lectures and provides few opportunities for the development of modern teaching skills. Our group developed an online tool called Adopt a Bacterium, which consists on a Facebook group where teacher assistants (TAs) can interact with students and have a first contact with student-focused learning approaches. This work shows the
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The outlier Pseudomonas aeruginosa strain ATCC 9027 harbors a defective LasR quorum-sensing transcriptional regulator. FEMS Microbiol. Lett. (IF 1.987) Pub Date : 2020-07-21 Selene García-Reyes,Martín P Soto-Aceves,Miguel Cocotl-Yañez,Abigail González-Valdez,Luis Servín-González,Gloria Soberón Chávez
Pseudomonas aeruginosa infections represent an important health problem that has been recognized by the World Health Organization as a research priority. A complex regulatory network called the quorum sensing (QS) regulates several P. aeruginosa virulence-related traits, including production of elastase, rhamnolipids and pyocyanin. The avirulent P. aeruginosa strain ATCC 9027 belongs to clade 3, which
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Archaea join the conversation: detection of AHL-like activity across a range of archaeal isolates. FEMS Microbiol. Lett. (IF 1.987) Pub Date : 2020-07-21 James Charlesworth,Onder Kimyon,Michael Manefield,Charlotte J Beloe,Brendan P Burns
Quorum sensing is a mechanism of genetic control allowing single cell organisms to coordinate phenotypic response(s) across a local population and is often critical for ecosystem function. Although quorum sensing has been extensively studied in bacteria comparatively less is known about this mechanism in Archaea. Given the growing significance of Archaea in both natural and anthropogenic settings,
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Optimization of scotta as growth medium to preserve biodiversity and maximise bacterial cells concentration of natural starter cultures for Pecorino Romano PDO cheese. FEMS Microbiol. Lett. (IF 1.987) Pub Date : 2020-07-21 Luigi Chessa,Antonio Paba,Elisabetta Daga,Marco Caredda,Roberta Comunian
Preservation of cheese microbiota biodiversity is central to the sensory quality of traditional and PDO cheeses. Lyophilized commercial selected starters, being advantageous in terms of cells concentration, are supplanting natural cultures causing important loss of microbial biodiversity in the dairy environment. Biodiversity could be recovered using natural starter cultures, however their cells concentration
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Membrane transporters in the bioproduction of organic acids: state of the art and future perspectives for industrial applications. FEMS Microbiol. Lett. (IF 1.987) Pub Date : 2020-07-18 I Soares-Silva,D Ribas,M Sousa-Silva,J Azevedo-Silva,T Rendulić,M Casal
Organic acids such as monocarboxylic acids, dicarboxylic acids or even more complex molecules such as sugar acids, have displayed great applicability in the industry as these compounds are used as platform chemicals for polymer, food, agricultural and pharmaceutical sectors. Chemical synthesis of these compounds from petroleum derivatives is currently their major source of production. However, increasing
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Antibacterial effects of plant extracts with hurdle technology against Vibrio cholerae. FEMS Microbiol. Lett. (IF 1.987) Pub Date : 2020-07-16 Tabitha Mlowoka Kayira,Hiroyuki Nakano
Vibrio cholerae (V. cholerae) is an etiological cause of cholera implicated in several pandemics. Antibacterial activity of plant extracts has been established. However, these extracts exhibit activity at a concentration which may alter organoleptic attributes of water and food, hence limiting their application. In this light, there is need to device ways of reducing plant extracts effective levels
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Expression of the Campylobacter jejuni FliD protein and its reaction to chicken sera. FEMS Microbiol. Lett. (IF 1.987) Pub Date : 2020-07-13 Xiao-Yan Zhang,Qian Zhou,Meng-Jun Tang,Jun-Hua Pu,Yan-Feng Fan,Jun-Xian Lu,Jin-Lin Huang,Yu-Shi Gao
Campylobacter is a leading causative pathogen of acute bacterial gastroenteritis among humans. Contaminated chicken products are regarded as major sources of human infection. The flagellar capping protein (FliD), which plays important roles in colonization and adhesion to the mucosal surface of chicken caeca, is conserved among Campylobacter jejuni strains. In this study, the recombinant C. jejuni