-
In‐tube solid‐phase microextraction of polycyclic aromatic hydrocarbons from refinery water samples using UiO‐66/polyacrylonitrile electrospun nanofibers followed by high‐performance liquid chromatography‐ultraviolet detection J. Sep. Sci. (IF 2.8) Pub Date : 2024-09-14 Seyedeh Sara Nasrollahi, Yadollah Yamini
A simple and quick fiber‐in‐tube solid‐phase microextraction (FIT‐SPME) was introduced for the extraction and determination of nine polycyclic aromatic hydrocarbons followed by a high‐performance liquid chromatography‐ultraviolet detector in refinery water samples. For this purpose, a water‐resistant metal‐organic framework with a high surface area called UiO‐66 has been applied in the form of an electrospun
-
-
The analysis of e‐liquids: A study on chemical diversity and metal content using gas chromatography‐mass spectrometry and inductively coupled plasma‐mass spectrometry J. Sep. Sci. (IF 2.8) Pub Date : 2024-09-11 Matjaž Rantaša, David Majer, Matjaž Finšgar
This work aimed to determine the chemical composition of 22 e‐liquids available on the Slovenian market. Four different gas chromatography (GC) sample introduction techniques; headspace‐GC‐mass spectrometry (HS‐GC‐MS), liquid injection‐GC‐MS (LI‐GC‐MS), HS‐solid‐phase microextraction‐GC‐MS (HS‐SPME‐GC‐MS), and direct‐immersion‐SPME‐GC‐MS (DI‐SPME‐GC‐MS) were employed for qualitative analysis. Various
-
Low swelling and high mechanical strength organosilane hybrid polydivinylbenzene microspheres for hydrophilic interaction chromatography applications J. Sep. Sci. (IF 2.8) Pub Date : 2024-09-10 Yang Yang, Yuexin Li, Yang Zhao, Changyu Cai, Xinmiao Liang, Yanxiong Ke
In this work, monodisperse organosilane hybrid polymer microspheres with a particle size of about 5 µm were synthesized using seed swelling polymerization. The organosilicon reagent methacryloxypropyltrimethoxysilane was introduced into the polymer framework as a copolymerization monomer, and the crosslinking degree of the microspheres was improved by the hydrolysis‐condensation reaction of siloxanes
-
Construction of amine‐rich coating utilizing amine curing reaction of triglycidyl isocyanate with triethylenetetramine on the surface of poly(styrene‐divinylbenzene) microspheres for preparation of anion exchange stationary phase J. Sep. Sci. (IF 2.8) Pub Date : 2024-09-10 Junwei Liu, Yuqi Yan, Haojie Li, Shiting Zhang, Guoqing Wang, Yu‐an Sun, Yan Zhu
Epoxy resins, as important thermosetting polymers, exhibit excellent adhesion to various substrates. In view of this, reticulate coating of triglycidyl isocyanate with triethylenetetramine was introduced onto the surface of poly(styrene‐divinylbenzene) utilizing amine curing reaction to obtain poly(styrene‐divinylbenzene)@triglycidyl isocyanate‐triethylenetetramine composite microspheres. The amino
-
Development of a pre‐column derivatization ultra‐high‐performance liquid chromatography method for polysaccharide and monosaccharide quantification in Lycium barbarum J. Sep. Sci. (IF 2.8) Pub Date : 2024-09-05 Zhilong Zhao, Yunfeng Bai, Huan Chen, Weibiao Wang, Shuqin Ding, Yue Zhang, Yuping Sa, Guoning Chen, Xueqin Ma
Given the limited specificity and accuracy observed in the current official colorimetric quantification of polysaccharide in Lycium barbarum, our study aims to establish a novel, specific, accurate, and economic pre‐column derivatization ultra‐high‐performance liquid chromatography (UHPLC) method for determining the monosaccharide and polysaccharide content in L. barbarum. The optimization of extraction
-
A Dual Membrane Electrodialytic Phosphoric Acid Eluent Generator for Ion Exclusion Chromatography-A Gas-Free Electrodialytic Phosphoric Acid Generator. J. Sep. Sci. (IF 2.8) Pub Date : 2024-09-01 Xiaolu Sun,Jingwen Tang,Weiqing Chen,Feifang Zhang,Bingcheng Yang
Relative to suppressed conductometric anion exchange chromatography, conductometric ion exclusion chromatography (IEC) is much less popular. Poor limit of detection and manual preparation of eluent are primarily responsible for this. We describe an electrodialytic phosphoric eluent generator for the online production of phosphoric acid eluent at a concentration exceeding 35 mM. It is a sandwiched configuration
-
Recent advances in liquid chromatography-tandem mass spectrometry for the detection of thyroid hormones and thyroglobulin in clinical samples: A review. J. Sep. Sci. (IF 2.8) Pub Date : 2024-09-01 Yuting Jin,Taiyu Zhai,Ying Wang,Jiuyan Li,Tingting Wang,Jing Huang
Thyroid hormones (THs), including triiodothyronine (T3), thyroxine (T4), and their metabolites, are essential for regulating development, growth, and energy metabolism. Thyroglobulin (Tg) produced by thyroid follicular cells acts as an essential substrate for TH synthesis. The combination of THs with Tg is a widely used serological laboratory test for thyroid function assessment. Early detection and
-
Prediction model for retention volumes of the three‐phase solvent system in high‐speed countercurrent chromatography J. Sep. Sci. (IF 2.8) Pub Date : 2024-08-31 Jules Muhire, Fu‐Xin Zhang, Xiao Sun, Xing‐Cui Wang, Dong Pei, Xin‐Yi Huang, Duo‐Long Di
Owing to its ability to separate substances with a broad scope of polarities, exploring the three‐phase solvent systems (TPSSs) with high‐speed countercurrent chromatography is a topic of interest in separation science, and their retention volumes should be more concerned. This study primarily investigates the behavior of retention volumes while examining the isolation abilities of the TPSS in the
-
A comprehensive strategy of lipidomics and pharmacokinetics based on ultra‐high‐performance liquid chromatography‐mass spectrometry of Shaoyao Gancao Decoction J. Sep. Sci. (IF 2.8) Pub Date : 2024-08-31 Xin Li, Juan Xie, Yuhan Li, Wenxuan Cui, Tongrui Zhang, Qing Li, Kaishun Bi, Ran Liu
Shaoyao Gancao Decoction (SGD), a traditional Chinese medicine, has been proven to have a good liver protection effect, but the mechanism and pharmacodynamic substances of SGD in the treatment of acute liver injury are still unclear. In this study, an ultra‐high‐performance liquid chromatography‐quadrupole‐time‐of‐flight mass spectrometry (UHPLC‐Q‐TOF‐MS) method was established to characterize 107
-
Development of analytical method for determination of 1,3‐dimethylamylamine in sports nutrition and bodybuilding supplements using pH‐switchable deep eutectic solvents followed by high‐performance liquid chromatography‐diode array detector J. Sep. Sci. (IF 2.8) Pub Date : 2024-08-31 Dijun Shen, Jie Zhang, Ying Zhang, Xiru Du
In this work, an easy, safe, simple, and efficient pH‐switchable deep eutectic solvents (DESs)‐based liquid phase microextraction followed by high‐performance liquid chromatography‐diode array detector analysis was developed for the determination of 1,3‐dimethylamylamine (DMAA). The switchability of the obtained DESs was investigated by changing the pH. Then the best‐selected DES was characterized
-
Pseudomorphic synthesis of pore size‐tunable mesoporous silica spherical particles and their application for the fraction of low‐molecular‐weight heparin J. Sep. Sci. (IF 2.8) Pub Date : 2024-08-30 Yang Zhao, Jie Li, Yang Yang, Yujie Bi, Changyu Cai, Yanxiong Ke
In this study, spherical silica with pore size varied from 30 to 200 Å was synthesized by pseudomorphic transformation at atmospheric pressure. 40–80 Å silica particles with a narrow pore distribution were obtained by using quaternary amine cationic surfactants and different kinds of swelling agents, including polypropylene glycol, 1,3,5‐trimethylbenzene, alkanes, and alkanols. Alkyl imidazolium ionic
-
-
Characterization of a mixed mode fluorocarbon/weak anion exchange sorbent for the separation of perfluoroalkyl substances J. Sep. Sci. (IF 2.8) Pub Date : 2024-08-28 Aghogho A. Olomukoro, Ruichao Xie, Fabiola X. Fernandez Paucar, Charlotte DeRosa, Neil D. Danielson, Emanuela Gionfriddo
The ubiquitous presence and persistence of per‐ and polyfluoroalkyl substances (PFAS) in the environment have raised concerns in the scientific community. Current research efforts are prioritizing effective PFAS remediation through novel sorbents with orthogonal interaction mechanisms. Recognized sorption mechanisms between PFAS and sorbents include hydrophobic, electrostatic, and fluorine‐fluorine
-
Determination of amino acids and peptides without their pre‐column derivatization by capillary electrophoresis with ultraviolet and contactless conductivity detection. An overview J. Sep. Sci. (IF 2.8) Pub Date : 2024-08-27 Liudmila Kartsova, Anastasiia Maliushevska
This review provides an overview of recent works focusing on the determination of amino acids (AAs) and peptides using capillary electrophoresis with contactless conductivity detection and ultraviolet (UV) detection, which is the most widespread detection in capillary electromigration techniques, without pre‐capillary derivatization. Available options for the UV detection of these analytes, such as
-
Research progress on pretreatment technology for the analysis of amphetamine biological samples J. Sep. Sci. (IF 2.8) Pub Date : 2024-08-27 Zebin Cao, Jianping Zhou
Sample pretreatment technology is crucial for drug analysis and detection, because the effect of sample pretreatment directly determinates the final analysis results. In recent years, with the continuous innovation of microextraction and other technologies like material preparation technologies and assistant technologies for extraction, the sample pretreatment techniques in the process of drug analysis
-
Evaluation of fluorous affinity using fluoroalkyl‐modified silica gel and selective separation of poly‐fluoroalkyl substances in organic solvents J. Sep. Sci. (IF 2.8) Pub Date : 2024-08-27 Atsuya Tadano, Yoshiyuki Watabe, Tetsuya Tanigawa, Sayaka Konishi‐Yamada, Takuya Kubo
In this study, we focused on the fluorous affinity acting among fluorine compounds, and then developed a new separation medium and evaluated their performance. We prepared the stationary phases for a column using silica gel‐modified alkyl fluoride and investigated the characteristics of fluorous affinity by comparing them with a typical stationary phase, which does not contain fluorine, using high‐performance
-
Generalized flow calculation of the gas flow in a network of capillaries used in gas chromatography J. Sep. Sci. (IF 2.8) Pub Date : 2024-08-23 Jan Leppert, Tillman Brehmer, Peter Boeker, Matthias Wüst
A general method for the calculation of the flow and pressure of a gas in a network of cylindrical capillaries is presented. This method is used specifically for gas chromatographic systems in this work. With this approach, it is possible to easily calculate flow and pressures in complex gas chromatographic systems, like flow‐modulated or thermal‐modulated multidimensional gas chromatographic systems
-
Advances in the selection of functional monomers for molecularly imprinted polymers: A review J. Sep. Sci. (IF 2.8) Pub Date : 2024-08-22 Jiaqi Niu, Min Du, Wanqing Wu, Jianlei Yang, Qing Chen
Molecularly imprinted polymers, a type of special polymer materials, are widely used in biosensing and other fields due to their ability to specifically recognize target molecules, often called “artificial receptors.”. Nowadays, researchers are constantly exploring new design and synthesis methods for molecularly imprinted materials to improve the selectivity and sensitivity of molecularly imprinted
-
Determination of xanthophylls in egg yolk using a combination of spectrophotometric and diol phase high‐performance liquid chromatography methods J. Sep. Sci. (IF 2.8) Pub Date : 2024-08-22 Anh Van Nguyen, Victor Ivanovic Deineka, T. G. Burzhinskaya, Irina Petrovna Blinova, Lyudmila Aleksandrovna Deineka, Anh Thi Ngoc Vu
Normal‐phase (NP) liquid chromatography is one of the most effective methods for separating isomers with sensitive structural features, including xanthophyll isomers. In this work, reverse‐phase (RP) and NP liquid chromatography (LC), with silica gel and diol phase, respectively, were evaluated for the separation of xanthophyll isomers. The results showed that RP LC with monomeric C18 phase not only
-
Substantial benefits of an inert biphenyl column for the analysis of steroids and their phase II metabolites in biological samples J. Sep. Sci. (IF 2.8) Pub Date : 2024-08-22 Mathieu Galmiche, Marie‐Anaïs Monat, Diego A. Lopez, Cyrille Lamboley, Paul Connolly, Sergey Girel, Davy Guillarme, Isabel Meister, Serge Rudaz
Steroids can be used as biomarkers in clinical metabolomics and other fields related to human toxicology. This chemical group is known for its complexity, considering its number of isobaric compounds and the wide variety of phases I and II metabolic pathways that parent compounds can undergo. For a successful analysis of steroids in biological samples, liquid chromatography separation must be finely
-
Feature‐based molecular networking with MS2LDA to profile compounds in Lanbuzheng based on ultra‐high‐performance liquid chromatography‐quadrupole Exactive Orbitrap high‐resolution mass spectrometry J. Sep. Sci. (IF 2.8) Pub Date : 2024-08-22 Xixian Kong, Guanghuan Tian, Tong Wu, Shaowei Hu, Jie Zhao, Fuzhu Pan, JingTong Liu, Yi OuYang, Liying Tang, Hongwei Wu
Lanbuzheng (Geum japonicum Thunb. var. chinense Bolle), a plant found in Southwest China, is a traditional Chinese medicine that promotes hematopoiesis and antioxidant functions. Many of its chemical constituents remain unknown, posing challenges both to understanding its pharmacological mechanisms and to conducting quality control research. In this work, ultra‐high performance liquid chromatography
-
Hydrophilic materials based on hydrogel polymers for enrichment of glycopeptides from serum of colorectal cancer patients J. Sep. Sci. (IF 2.8) Pub Date : 2024-08-22 Xiqian Zhang, Danni Wang, Jiahui Shao, Haijiao Mao, Chuan‐Fan Ding, Yinghua Yan
In this work, a composite hydrogel material consisting of chitosan‐based composite hydrogel was prepared by a simple and rapid synthetic method and will be named three‐dimensional (3D)‐IL‐COF‐1@CS hydrogel. Possessing a stable 3D network structure and outstanding hydrophilicity, the novel hydrogel is capable of capturing glycopeptides. The 3D‐IL‐COF‐1@CS hydrogel showed good sensitivity (0.1 fmol/µL)
-
Analysis of nitrogen‐containing rocket fuel transformation products in loamy soil by thermal desorption gas chromatography‐tandem mass spectrometry J. Sep. Sci. (IF 2.8) Pub Date : 2024-08-16 Mark S. Popov, Dmitry S. Kosyakov, Nikolay V. Ul'yanovskii
Unsymmetrical dimethylhydrazine (UDMH) based rocket fuel, when released into the soil, undergoes oxidative transformations to form a variety of toxic nitrogen‐containing products (TPs). Loamy soils containing aluminosilicates (clay) are capable of strong binding and retention of UDMH TPs due to a combination of polar sorption and cation‐exchange properties, posing challenges for their extraction and
-
-
Comprehensive review of combustion ion chromatography for the analysis of total, adsorbable, and extractable organic fluorine J. Sep. Sci. (IF 2.8) Pub Date : 2024-08-13 Charles Phillip Shelor, Cable Warren, Chimauchem V. Odinaka, Kalpana Dumre
Poly‐ and perfluoroalkyl substances (PFAS) are a class of persistent organic pollutants whose high stability and appreciable water solubility have led to near‐global contamination. PFAS are bioaccumulative toxins that have been linked to a myriad of disorders and have been detected nearly universally in human blood. Liquid chromatography‐tandem mass spectrometry is the most frequent method used for
-
Multiclass and multi‐residue determination of pesticides and antibiotics in sediment from an aquacultural environment based on modified dispersive solid‐phase extraction J. Sep. Sci. (IF 2.8) Pub Date : 2024-08-13 Zhuozhen Qian, Shuifen Tang, Zhiyu Liu, Fangfang Luo, Yanting Chen, Shaohong Wei
A quick, easy, cheap, effective, rugged, and safe method was developed for the multi‐residue analysis of pesticides and antibiotics in aquaculture sediment using high‐performance liquid chromatography‐tandem mass spectrometry (HPLC‐MS/MS). The developed method is based on ultrasonic extraction with acetonitrile and phosphate buffer, salting with sodium chloride, and cleaning with dispersive solid‐phase
-
Macroporous polymeric resin for the purification of flavonoids from medicinal plants: A review J. Sep. Sci. (IF 2.8) Pub Date : 2024-08-13 Raghad Yousef Ali Aljawarneh, Mohamad Shazeli Che Zain, Fauziahanim Zakaria
The purification of flavonoids using the macroporous polymer resin method has gained attention in recent years due to its simplicity, precision, cost‐effectiveness, and the ability to separate flavonoids from other constituents. Several studies have been conducted to investigate the efficiency and effectiveness of macroporous polymer resin in purifying flavonoids from various plant sources. This review
-
Recent progress of porous cage materials in sample preparation, chromatographic separation, and detection J. Sep. Sci. (IF 2.8) Pub Date : 2024-08-09 Hongzhi Hu, Ling Xia, Gongke Li, Yi Chen
Porous cage materials with certain dimensions, sizes, shapes, and functions have been regarded as promising materials for sample preparation, chromatographic separation, and detection process. In contrast to infinite frameworks such as metal‐organic frameworks or covalent organic frameworks, porous cage materials are constructed from discrete molecules containing at least one internal cavity. The well‐defined
-
Room temperature synthesis of a chiral covalent organic framework core‐shell composite for high‐performance liquid chromatography enantioseparation J. Sep. Sci. (IF 2.8) Pub Date : 2024-08-07 Yue Zhang, Yan‐Rui Lu, Cheng Liu, An‐Xu Ma, Zong‐Hong Luo, Hong‐Mei Zhou, Jun‐Hui Zhang, Bang‐Jin Wang, Sheng‐Ming Xie, Li‐Ming Yuan
In this article, chiral covalent organic framework core‐shell composite CCOF‐TpPa‐Py@SiO2 was facilely synthesized by induction at room temperature. The CCOF‐TpPa‐Py@SiO2 core‐shell composite was used as a chiral stationary phase for the separation of the racemates by high‐performance liquid chromatography, which exhibits good separation performance for chiral compounds including ketones, alcohols
-
Electromembrane extraction of peptides based on charge, hydrophobicity, and size – A large‐scale fundamental study of the extraction window J. Sep. Sci. (IF 2.8) Pub Date : 2024-08-02 Torstein Kige Rye, Chien‐Yun Lee, Andreas Zellner, Sara Haglund Moen, Samira Dowlatshah, Trine Grønhaug Halvorsen, Stig Pedersen‐Bjergaard, Frederik André Hansen
This study investigated the capability of electromembrane extraction (EME) as a general technique for peptides, by extracting complex pools of peptides comprising in total of 5953 different substances, varying in size from seven to 16 amino acids. Electromembrane extraction was conducted from a sample adjusted to pH 3.0 and utilized a liquid membrane consisting of 2‐nitrophenyl octyl ether and carvacrol
-
Screening and isolation of quorum sensing inhibitors of Pseudomonas aeruginosa from Phellodendron amurense extracts using bio‐affinity chromatography J. Sep. Sci. (IF 2.8) Pub Date : 2024-08-02 Yu Yi, Ye Zhou, Susu Lin, Kefan Shi, Jianfeng Mei, Guoqing Ying, Shujiang Wu
Drug‐resistant bacterial infections pose a significant challenge in the field of bacterial disease treatment. Finding new antibacterial pathways and targets to combat drug‐resistant bacteria is crucial. The bacterial quorum sensing (QS) system regulates the expression of bacterial virulence factors. Inhibiting bacterial QS and reducing bacterial virulence can achieve antibacterial therapeutic effects
-
Ultrasound‐assisted matrix solid‐phase extraction based on deep eutectic solvents and zinc oxide: Extraction and determination of six active ingredients in Ligustri Lucidi Fructus J. Sep. Sci. (IF 2.8) Pub Date : 2024-08-02 Chengjialu Qian, Shizhao Wang, Hongyan Chen, Jiankuan Li
In this study, we propose a novel strategy utilizing deep eutectic solvents (DESs) as both the extraction solvent and dispersing liquid, with nanometer zinc oxide (ZnO) serving as the adsorbent. This method incorporates ultrasound‐assisted matrix solid phase dispersion (UA‐MSPD) for the extraction of six active components (salidroside, echinacoside, acteoside, specnuezhenide, nuezhenoside G13, and
-
-
An ultra‐fast ultra‐high‐performance liquid chromatography‐tandem mass spectrometry method for estimating the in vitro metabolic stability of palbociclib in human liver microsomes: In silico study for metabolic lability, absorption, distribution, metabolism, and excretion features, and DEREK alerts screening J. Sep. Sci. (IF 2.8) Pub Date : 2024-08-01 Mohamed W. Attwa, Ali S. Abdelhameed, Adnan A. Kadi
Palbociclib (Ibrance; Pfizer) was approved for the management of metastatic breast cancer characterized by hormone receptor‐positive/human epidermal growth factor receptor 2 negative status. The objective of this study was to create a fast, precise, environmentally friendly, and highly sensitive ultra‐high‐performance liquid chromatography‐tandem mass spectrometry approach for quantifying palbociclib
-
An ultra‐fast green ultra‐high‐performance liquid chromatography‐tandem mass spectrometry method for estimating the in vitro metabolic stability of zotizalkib in human liver microsomes J. Sep. Sci. (IF 2.8) Pub Date : 2024-08-01 Mohamed W. Attwa, Ali S. Abdelhameed, Adnan A. Kadi
Zotizalkib (ZTK, TPX‐0131) is a fourth‐generation highly effective inhibitor of wild‐type anaplastic lymphoma kinase (ALK) and ALK‐resistant mutations that can penetrate the central nervous system. It exhibited greater potency compared to all five officially approved ALK inhibitors. The aim of this study was to develop a rapid, accurate, eco‐friendly, and highly sensitive ultra‐high‐performance liquid
-
Magnetic materials as adsorbents for the pre‐concentration and separation of active ingredients from herbal medicine J. Sep. Sci. (IF 2.8) Pub Date : 2024-07-29 Hezhao Bai, Guohua Teng, Chen Zhang, Jingyi Yang, Wenzhi Yang, Fei Tian
Herbal medicine (HM) is crucial in disease management and contains complex compounds with few active pharmacological ingredients, presenting challenges in quality control of raw materials and formulations. Effective separation, identification, and analysis of active components are vital for HM efficacy. Traditional methods like liquid‐liquid extraction and solid‐phase extraction are time‐consuming
-
Hydrophilic interaction chromatographic evaluation of zwitterionic polymer grafted silica gel via multiple binding sites J. Sep. Sci. (IF 2.8) Pub Date : 2024-07-26 Pengcheng Zhang, Yongxing Hu, Kunling Liu, Yaming Sun, Lijun He, Wenjie Zhao
A novel zwitterionic polymer grafted silica stationary phase, Sil‐PZIC, was prepared by bonding poly(ethylene maleic anhydride) molecules on the surface of silica via multiple binding sites, followed by ammonolysis of maleic anhydride through a nucleophilic substitution reaction with ethylenediamine. The stationary phase was characterized by solid‐state 13C nuclear magnetic resonance, zeta potential
-
Nickel‐ion substituted hydroxyapatite matrices for metal‐affinity chromatographic purification of recombinant proteins J. Sep. Sci. (IF 2.8) Pub Date : 2024-07-26 Anbuthangam Ashokan, T. S. Sampath Kumar, Guhan Jayaraman
Hydroxyapatite (HAp) is a calcium phosphate ceramic, widely used as a matrix for protein chromatography. The crystal structure of HAp is amenable to a wide range of substitutions, thus allowing for the alteration of its properties. In this study, nickel‐ion substituted HAp (NiSHAp) was synthesized using a wet‐precipitation method, followed by spray drying. This resulted in the structural incorporation
-
Development and validation of stability indicating assay method for mitapivat: Identification of novel hydrolytic, photolytic, and oxidative forced degradation products employing quadrupole‐time of flight mass spectrometry J. Sep. Sci. (IF 2.8) Pub Date : 2024-07-26 Manasi Ashok Bagul, Yatesh Patil, Sayalee Sanjay Mane, Anandhu Kunnath Shaji, Pintu Das, Om Prakash Ranjan, Swapnil Jayant Dengale
Mitapivat is a novel, first‐in‐class orally active pyruvate kinase activator approved by the US Food and Drug Administration in 2022 for the treatment of hemolytic anemia. There is no literature available regarding the identification of degradation impurities of mitapivat. The present study deals with the degradation behavior of mitapivat under various stress conditions such as hydrolytic, photolytic
-
Electrospun nanofibers‐based thin film microextraction for enrichment of phthalate esters in biodegradable plastics J. Sep. Sci. (IF 2.8) Pub Date : 2024-07-22 Ping Su, Qiqi Wang, Mengxi Li, Xinrui Tian, Jiayi Song, Yi Yang
In this work, a novel electrospun nanofiber (PAN/TpBD; 2,4,6‐triformylphloroglucinol [Tp] and benzidine [BD]; polyacrylonitrile [PAN]) was fabricated via a facile electrospinning method and utilized as adsorbent in thin film microextraction (TFME) of phthalate esters (PAEs) (dimethyl phthalate, diethyl phthalate, diallyl phthalate, dibutyl phthalate, and dioctyl phthalate) in biodegradable plastics
-
Rapid identification of chemical components and screening of acetylcholinesterase inhibitors from Dalbergia odorifera based on mass defect and diagnostic ion filtering strategy, affinity ultrafiltration, and liquid chromatography‐tandem mass spectrometry J. Sep. Sci. (IF 2.8) Pub Date : 2024-07-22 Hongbin Zhang, Yuecheng Liu, Ling Zhang, Zhenhua Tian, Hui Zhang, Haiqiang Jiang
Dalbergia odorifera is a natural product rich in pharmacological ingredients, but the comprehensive characterization and rapid profiling of active components remain a challenge. Thus, an integrated data mining and identification strategy was exploited to efficiently identify the chemical constituents and screen acetylcholinesterase inhibitors (AChEIs) through affinity ultrafiltration and ultra‐high‐performance
-
Evaluation and comparison of liquid chromatography/high‐resolution mass spectrometry platforms for the separation and characterization of ginsenosides from the leaves of Panax ginseng J. Sep. Sci. (IF 2.8) Pub Date : 2024-07-22 Xiaohang Li, Yadan Zou, Huizhen Cheng, Mengxiang Ding, Yang Yang, Lili Hong, Ying Xiong, Min Zhang, Xue Li, Qinhua Chen, Hongda Wang, Yuanwu Cui, Wenzhi Yang
The measurement of data repeatability in small‐molecule metabolites acquired within and among different liquid chromatography‐mass spectrometry (LC‐MS) platforms is crucial for data sharing or data transfer in natural products research. This work was designed to investigate and evaluate the separation and detection performance of three commercial high‐resolution LC‐MS platforms (e.g., Agilent 6550
-
Multiple analyses of main flavor components in reconstituted tobacco and transfer behavior of their key substances during heating J. Sep. Sci. (IF 2.8) Pub Date : 2024-07-22 Jianyang Shi, Yunxia Yang, Ting Zhang, Kun Liang, Linqing Guo, Ruijie Deng, Kai Liu, Yao Ren
Reconstituted tobacco (RT) is a product made by reprocessing tobacco waste, experiencing a growing demand for heat‐not‐burn products. The purpose of this study is to analyze the main flavor ingredients in RT aerosol, as well as the transfer behavior of key flavor substances from substrates to aerosol and the concentrations of these compounds in the substrate after heating. First, we demonstrated that
-
Magnetic molecularly imprinted polymer based on coordination for the determination of trace banned substance furosemide in human urine J. Sep. Sci. (IF 2.8) Pub Date : 2024-07-22 Kunlin He, Qian Chen, Xueling Chen, Chungu Zhang, Shun Feng, Lianhai Shan
Furosemide (FUR), banned in sports events by the World Anti‐Doping Agency, is a key target in drug tests, necessitating a pretreatment material capable of selectively, rapidly, and sufficiently separating/enriching analytes from complex matrices. Herein, a metal‐mediated magnetic molecularly imprinted polymer (mMIP) was rationally designed and synthesized for the specific capture of FUR. The preparations
-
Indirect and sensitive determination of microRNAs by magnetic field‐assisted capillary sieving electrophoresis combined with catalytic hairpin assembly J. Sep. Sci. (IF 2.8) Pub Date : 2024-07-22 Jinfeng Ning, Junlan Ye, Qingqing Wang, Wei Wang
To determine multiple microRNAs (miRNAs) from cells simultaneously is essential for understanding biological functions. Capillary electrophoresis (CE) can simultaneously determine multiple miRNAs by separation. Nevertheless, similar lengths and low concentrations in cells make miRNAs hard to separate and detect. In this study, CE with laser‐induced fluorescence detection was combined with catalytic
-
Correction to “A microwave‐assisted extraction method combined with magnetic ionic liquid‐based dispersive liquid–liquid microextraction for the extraction of chloramine–T from fish samples prior to its determination by high‐performance liquid chromatography” J. Sep. Sci. (IF 2.8) Pub Date : 2024-07-22
-
-
Fast screening of α‐glucosidase inhibitors from Ginkgo biloba leaf by using α‐glucosidase immobilized on magnetic metal‐organic framework J. Sep. Sci. (IF 2.8) Pub Date : 2024-07-19 Yue Li, Hongmei Liu, Sikai Wang, Wei Fang, Xinxin Jiang, Guoqi Zhang, Yan Zhao
In this study, a ligand fishing method for the screening of α‐glucosidase inhibitors from Ginkgo biloba leaf was established for the first time using α‐glucosidase immobilized on the magnetic metal‐organic framework. The immobilized α‐glucosidase exhibited enhanced resistance to temperature and pH, as well as good thermal stability and reusability. Two ligands, namely quercitrin and quercetin, were
-
Preparative separation of the constituents from Ginkgo biloba L. leaves by a combination of multi‐stage solvent extraction and countercurrent chromatography J. Sep. Sci. (IF 2.8) Pub Date : 2024-07-15 Zhuo Liu, Peng Tian, Luqi Li, Jinxing Guo, Yi Zhou, Shuhui Liu, Zhi Yang
In this study, we employed a combination approach for the preparative separation of constituents from Ginkgo biloba L. leaves. It involved multi‐stage solvent extractions utilizing two‐phase multi‐solvent systems and countercurrent chromatography (CCC) separations using three different solvent systems. The n‐heptane/ethyl acetate/water (1:1:2, v/v) and n‐heptane/ethyl acetate/methanol/water (HepEMWat
-
Quantification of amino acids secreted by yeast cells by hydrophilic interaction liquid chromatography‐tandem mass spectrometry J. Sep. Sci. (IF 2.8) Pub Date : 2024-07-10 Xiongwei Yin, Luís Santos Sousa, Bruno André, Erwin Adams, Ann Van Schepdael
Monitoring the levels of amino acids (AAs) in biological cell cultures provides key information to understand the regulation of cell growth and metabolism. Saccharomyces cerevisiae can naturally excrete AAs, making accurate detection and determination of amino acid levels within the cultivation medium pivotal for gaining insights into this still poorly known process. Given that most AAs lack ultraviolet
-
Atypical applications of transverse diffusion of laminar flow profiles methodology for in‐capillary reactions in capillary electrophoresis J. Sep. Sci. (IF 2.8) Pub Date : 2024-07-10 Taťána Bržezická, Lenka Kohútová, Zdeněk Glatz
Capillary electrophoresis (CE) is a powerful separation technique offering quick and efficient analyses in various fields of bioanalytical chemistry. It is characterized by many well‐known advantages, but one, which is perhaps the most important for this application field, is somewhat overlooked. It is the possibility to perform chemical and biochemical reactions at the nL scale inside the separation
-
A comprehensive strategy based on ultra‐high performance liquid chromatography with diode array detector fingerprinting and multi‐component ultra‐performance liquid chromatography with tandem mass spectrometry technology for quality control of Jiawei Huoxiang Zhengqi Pill J. Sep. Sci. (IF 2.8) Pub Date : 2024-07-10 Yang Lu, Ting Lei, Xinyang Chen, Na Ning, Qiuling Huang, Xu Wu, Shengpeng Wang, Peng Li, Li Wan, Jiliang Cao
Jiawei Huoxiang Zhengqi Pill (JHZP) is a commonly used Chinese patent medicine for the clinical treatment of headache, dizziness, chest tightness as well as abdominal distension, and pain caused by wind‐cold flu. In this study, a comprehensive strategy combining ultra‐high performance liquid chromatography with diode array detector (UHPLC‐DAD) fingerprinting and multi‐component quantitative analysis
-
Recent progress for chiral stationary phases based on chiral porous materials in high‐performance liquid chromatography and gas chromatography separation J. Sep. Sci. (IF 2.8) Pub Date : 2024-07-05 Zhen Wang, Wei Wang, Ai‐Qin Luo, Li‐Ming Yuan
Chirality is a fundamental property of nature. Separation and analysis of racemates are of great importance in the fields of medicine and the production of chiral biopharmaceutical intermediates. Chiral chromatography has the characteristics of a wide separation range, fast separation speed, and high efficiency. The development and preparation of novel chiral stationary phases with good chiral recognition
-
One‐step enrichment and stepwise elution of glycoproteins and phosphoproteins by hydrophilic Ti4+‐immobilized dendrimer poly(glycidyl methacrylate) microparticles functionalized with polyethylenimine and phytic acid J. Sep. Sci. (IF 2.8) Pub Date : 2024-07-01 Hao Wang, Simin Li, Xinyan Wang, Xinyue Li, Minzhi Jiang, Shiying Chen, Zaiqi Hu, Haitian Li, Yuzhen Xu, Lei Jin
Glycosylation and phosphorylation rank as paramount post‐translational modifications, and their analysis heavily relies on enrichment techniques. In this work, a facile approach was developed for the one‐step simultaneous enrichment and stepwise elution of glycoproteins and phosphoproteins. The core of this approach was the application of the novel titanium (IV) ion immobilized poly(glycidyl methacrylate)
-
-
Determination of six short‐chain fatty acids in rat feces using headspace solid‐phase dynamic extraction coupled with GC‐MS J. Sep. Sci. (IF 2.8) Pub Date : 2024-06-28 Lihua Xing, Qiang Zhang, Juan Liu, Nianjun Yu, Yiqun Jia
Short‐chain fatty acids (SCFAs) are organic acids with carbon atoms less than six, released through fermentation products by intestinal microbiome, having multiple physiological activities. Considering weak acidity and high volatility, derivatization or liquid–liquid extraction is essential, which is time consuming. Headspace‐solid‐phase dynamic extraction (HS‐SPDE) coupled with gas chromatography‐mass
-
Exploring separation mechanisms and lipophilicity in hydrophilic interaction chromatography conditions by thin‐layer chromatography of anesthetics and adjuvant drugs as polar model compounds J. Sep. Sci. (IF 2.8) Pub Date : 2024-06-28 Aleksandra Radoičić, Sandra Šegan, Dušanka Milojković‐Opsenica
The chromatographic behavior of the selected compounds was studied under conditions of hydrophilic interaction liquid chromatography (HILIC). The effect of mobile phase composition on the retention in different chromatographic systems was systematically examined using high‐performance thin‐layer chromatography. The sorbents of different polarity and adsorption characteristics were selected and mixtures
-
Discovery and determination of misuse and chemotypes of Pogostemon cablin by liquid chromatography‐quadrupole time‐of‐flight mass spectrometry and liquid chromatography with diode‐array detector J. Sep. Sci. (IF 2.8) Pub Date : 2024-06-21 Chieh‐Chun Wei, Zi‐Rong Tseng, Hsiao‐Wei Liao
Traditional Chinese medicine (TCM) has garnered significant scientific interest in healthcare but faces increased regulatory scrutiny due to concerns about uncontrolled usage. This study focuses on characterizing Pogostemon cablin (PC) to mitigate potential misuse and identify chemotype differences. Leveraging untargeted metabolomics, we identified 222 distinctive features effectively differentiating
-
Modeling the liquid‐liquid chromatography separation of cannabinoids from hemp extracts J. Sep. Sci. (IF 2.8) Pub Date : 2024-06-21 Simon Vlad Luca, Melanie Gerigk, Ahmad Adeel Arshad, Mirjana Minceva
The separation of cannabinoids from hemp materials is nowadays one of the most promising industrial applications of liquid‐liquid chromatography (LLC). Despite various experimental research efforts to purify cannabinoids, there are currently few works on process modeling. Thus, this study aimed to explore a straightforward approach to model the LLC separation of cannabinoids from two hemp extracts