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High‐throughput extraction and automatic purification of alternariol from edible and medicinal herbs based on aptamer‐functionalized magnetic nanoparticles J. Sep. Sci. (IF 3.1) Pub Date : 2024-03-13 Ruiqi Yang, Liping Zhao, Fengchao Wang, Jin Chen, Xiao Ma, Yunxia Luan, Weijun Kong
Mycotoxin contamination is widespread in plants and herbs, posing serious threats to the consumer and human health. Of them, alternariol (AOH) has attracted great attention as an “emerging” mycotoxin in medicinal herbs. However, a specific and high‐throughput extraction method for AOH is currently lacking. Thus, developing an efficient pre‐treatment technique for AOH detection is extremely vital. Here
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Multi‐dimensional technology – Recent advances and applications for biotherapeutic characterization J. Sep. Sci. (IF 3.1) Pub Date : 2024-03-13 Thomas Bouvarel, Julien Camperi, Davy Guillarme
This review provides an overview of the latest advancements and applications in multi‐dimensional liquid chromatography coupled with mass spectrometry (mD‐LC‐MS), covering aspects such as inter‐laboratory studies, digestion strategy, trapping column, and multi‐level analysis. The shift from an offline to an online workflow reduces sample processing artifacts, analytical variability, analysis time,
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Porous poly(bismaleimide‐co‐divinylbenzene) microspheres as dispersive solid‐phase extraction adsorbent coupled to high‐performance liquid chromatography for the determination of triazine herbicide residues in vegetable samples J. Sep. Sci. (IF 3.1) Pub Date : 2024-03-13 Yang Xu, Jingyi Bao, Yuhan Ning, Weiping Wang, Ai‐jun Wang, Jiu‐ju Feng
In this work, monodisperse and nano‐porous poly(bismaleimide‐co‐divinylbenzene) microspheres with large specific surface area (427.6 m2/g) and rich pore structure were prepared by one‐pot self‐stable precipitation polymerization of 2,2′‐bis[4‐(4‐maleimidophenoxy) phenyl] propane and divinylbenzene. The prepared poly(bismaleimide‐co‐divinylbenzene) microspheres were employed as dispersive solid‐phase
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Kinematic viscosity prediction of jet fuels and alternative blending components via comprehensive two‐dimensional gas chromatography, partial least squares, and Yeo‐Johnson transformation J. Sep. Sci. (IF 3.1) Pub Date : 2024-03-13 Louis Edwards Caceres‐Martinez, Gozdem Kilaz
This work presents an accurate yet simplified partial least squares model to predict the kinematic viscosity of conventional and alternative jet fuels at −20°C using comprehensive two‐dimensional gas chromatography coupled to a flame ionization detector (GC × GC/FID). Three different normalization methods (mean‐centering, logarithmic, and Yeo‐Johnson) were evaluated to identify their impact in the
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Liquid chromatography‐mass spectrometry‐based strategy for systematic profiling of chemical components and associated quantitative analysis of quality markers in Qi‐Wei‐Tong‐Bi oral liquid J. Sep. Sci. (IF 3.1) Pub Date : 2024-03-13 Xiaojun Huang, Qingqing Fei, Sheng Yu, Rongli Qiu, Ting Geng, Xialin Chen, Liang Cao, Zhenzhong Wang, Mingqiu Shan
Qi‐Wei‐Tong‐Bi oral liquid (QWTB), a famous Chinese medicine preparation composed of seven crude drugs has a good therapeutic effect on rheumatoid arthritis and is widely used in China. However, its chemical composition and quality control have not been comprehensively and systematically investigated. In this study, high‐performance liquid chromatography coupled with quadrupole time‐of‐flight mass
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Covalent organic framework/layered double hydroxide composite–coated poly(ether ether ketone) jacket for stir bar sorptive extraction of Sudan dyes J. Sep. Sci. (IF 3.1) Pub Date : 2024-03-13 Zhenfu He, Shumin Ma, Peiqi Huang, Qionghuan Liang, Rong Wang
A novel coating for stir bar sorptive extraction was developed by growing a covalent organic framework, TpPa‐1 (derived from phenylenediamine and 1,3,5‐trimethylphloroglucinol), onto the surface of Ni‐Al layered double hydroxide. Using a poly(ether ether ketone) tube as the supporting substrate, a TpPa‐1/layered double hydroxide–coated stir bar was fabricated and demonstrated excellent extraction performance
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Ultra‐performance liquid chromatography‐quadrupole time‐of‐flight mass combined with UNIFI to study the mechanism of Tao Hong Si Wu Decoction in the treatment of postpartum blood stasis J. Sep. Sci. (IF 3.1) Pub Date : 2024-03-13 Mengdie Zhao, Wenwen Xia, Peiliang Zhang, Qingqing Xie, Wenyu Mu, Linfeng Tang, Zhuqing Liu, Lan Han, Daiyin Peng
Postpartum hemorrhage can lead to a variety of maternal complications. Tao Hong Si Wu Decoction (THSWD) is a traditional Chinese medicine used for treating gynecological diseases. However, the active ingredients of THSWD and its pharmacological mechanism of treatment for postpartum blood stasis still remained unclear. In this study, 201 components were identified in THSWD ethanol extract using ultra‐performance
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Deep eutectic solvent–based pressurized liquid extraction combined with dispersive liquid–liquid microextraction of organophosphorus pesticide residues in egg powder prior to high‐performance liquid chromatography analysis J. Sep. Sci. (IF 3.1) Pub Date : 2024-03-11 Omid Zareasghari, Afshin Javadi, Mohammad Reza Afshar Mogaddam
Herein, a deep eutectic solvent (DES)‐based miniaturized pressurized liquid extraction in combination with DES‐based dispersive liquid–liquid microextraction (DLLME) was developed for the extraction of organophosphorus pesticides (parathion–methyl, triazophos, parathion, diazinon, and phoxim) from egg powder samples prior to their analysis by a high‐performance liquid chromatography‐diode array detector
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Exploring the effect of Wuzhi capsule on the pharmacokinetics of regorafenib and its main metabolites in rat plasma using liquid chromatography‐tandem mass spectrometry J. Sep. Sci. (IF 3.1) Pub Date : 2024-03-11 Junfeng Zhu, Like Zhong, Yu Song, Haiying Ding, Wenxiu Xin, Gaoqi Xu, Luo Fang
Regorafenib is a small‐molecule tyrosine kinase inhibitor with severe hepatotoxicity. It undergoes metabolism mainly by CYP3A4 to generate active metabolites regorafenib‐N‐oxide (M2) and N‐desmethyl‐regorafenib‐N‐oxide (M5). Wuzhi capsule (WZC) is an herbal preparation derived from Schisandra sphenanthera and is potentially used to prevent regorafenib‐induced hepatotoxicity. This study aims to explore
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Analytical quality by design approach for the development of high‐performance liquid chromatography method for simultaneous analysis of metformin and sitagliptin in the presence of major degradation products J. Sep. Sci. (IF 3.1) Pub Date : 2024-03-11 Rajesh Pawar, Pothuraju Naresh, Bhadrayya Kalva, M. Raj Kumar, V. Ravichandiran, P. Ramalingam
An analytical quality by design‐based high‐performance liquid chromatography method for determining metformin (MET) and sitagliptin (SIT) in stress‐degraded samples was developed and validated. The analytical target profile and risk assessment‐driven critical method variables, for example, pH, % aqueous, and buffer concentration, were studied for their effect on method responses of retention time and
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Rapid screening and isolation of 5‐lipoxygenase inhibitors in Inonotus obliquus and mechanism of action in the treatment of asthma J. Sep. Sci. (IF 3.1) Pub Date : 2024-03-11 Haocheng Yun, Zhen Liu, Wanchao Hou, Qiang Liu, Yuyu Nong, Sainan Li, Chunming Liu
Accurate screening and targeted preparative isolation of active substances in natural medicines have long been two technical challenges in natural medicine research. This study outlines a new approach to improve the efficiency of natural product preparation, focusing on rapidly and accurately screening potential active ingredients in Inonotus obliquus as well as efficiently preparing 5‐lipoxidase (5‐LOX)
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Pharmacokinetics of four tannin compounds from Sanguisorba officinalis L. before and after processing by ultra‐high‐performance liquid chromatography‐tandem mass spectrometry J. Sep. Sci. (IF 3.1) Pub Date : 2024-02-26 Yuxin Wei, Chunjuan Yang, Shuang Jiang, Zhibin Wang
Sanguisorba officinalis L. possesses detoxifying, analgesic, and hemostatic properties. After charred processing, S. officinalis exhibits significantly enhanced medicinal effects. Currently, most pharmacokinetic studies focus on the chemical constituents of unprocessed S. officinalis. There is limited research on the comparison of chemical constituents before and after processing. This study established
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Rapid screening and target‐guided isolation of antioxidants from German chamomile by 2,2‐diphenyl‐1‐picrylhydrazyl‐ultra‐high‐performance liquid chromatography‐mass spectrometry coupled with off‐line two‐dimensional high‐speed countercurrent chromatography J. Sep. Sci. (IF 3.1) Pub Date : 2024-02-26 Wen Su, Shujing Jiang, Qi Liu, Conghao Sun, Xiaoqing Chen
German chamomile is one of the most effective herbal elements used in anti‐allergic products and as an antioxidant. Herein, the antioxidant activity of different extract fractions of German chamomile was initially evaluated using an off‐line 2,2‐diphenyl‐1‐picrylhydrazyl spectrophotometric assay. The ethyl acetate extract demonstrated the highest efficacy in scavenging free radicals. Based on this
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Simultaneous determination of selected ionophoric coccidiostats and amino acids in feed premixes using high‐performance liquid chromatography‐ultraviolet detection method J. Sep. Sci. (IF 3.1) Pub Date : 2024-02-26 Sahar Zayed, Fathalla Belal, Sona Barghash, Fatma Fouad
The combination of ionophoric coccidiostats and amino acids (AAs) is important in poultry feeding to enhance immunity and improve the growth and feed efficiency of birds suffering from coccidiosis. A simple, rapid, and economical high‐performance liquid chromatography‐ultraviolet detection (HPLC‐UV) method for the simultaneous determination of three ionophoric coccidiostats, namely salinomycin (SAL)
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Surface molecularly imprinted polymers based on magnetic multi‐walled carbon nanotubes for the highly selective purification of resveratrol from crude extracts of Vitis vinifera, Arachis hypogaea, and Polygonum cuspidatum J. Sep. Sci. (IF 3.1) Pub Date : 2024-02-26 Run‐Ze Cao, Qing‐Yan Gai, Jiao Jiao, Yuan Wang, Yu‐Jie Fu, Si‐Ming Nie, Yao Lu
In this work, surface molecularly imprinted polymers based on magnetic multi‐walled carbon nanotubes were prepared for the specific recognition and adsorption of resveratrol. The functionalization of magnetic multi‐walled carbon nanotubes and the synthesis process of surface molecularly imprinted polymers were optimized. Characterizations were performed to demonstrate the successful synthesis of the
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Evaluation of the key ingredient from the main production areas of Phellodendri Amurensis Cortex using ultra‐high‐performance liquid chromatography‐quadrupole time‐of‐flight tandem mass spectrometry and ultra‐high‐performance liquid chromatography coupled to triple‐quadrupole mass spectrometry J. Sep. Sci. (IF 3.1) Pub Date : 2024-02-26 Yuran Sun, Qiqi Zhao, Heng Fang, Hui Sun, Le Yang, Ye Sun, Guangli Yan, Ying Han, Xijun Wang
Phellodendri Amurensis Cortex (PAC) is a medicinal herb that has been generally used to treat diarrhea and jaundice. In order to comprehensively evaluate the PAC in the main production areas quality, a qualitative and quantitative method with highly effective, sensitive, and reliable was developed. The chemical compositions of PAC were analyzed, and fingerprints were established by ultra‐high‐performance
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Recycling of deep eutectic solvent in the extraction of ferulic acid from oil palm empty fruit bunch J. Sep. Sci. (IF 3.1) Pub Date : 2024-02-26 Mei Han Ng, Abdul Hadi Nu'man, Alias Hasliyanti
The study explored ferulic acid extraction from palm empty fruit bunch (EFB) fiber using deep eutectic solvent (DES) of chlorine chloride—acetic acid as the extraction medium and the way to recover and recycle the DES thereafter. Antisolvent was added to selectively precipitate the ferulic acid, which was recovered by filtration thereafter. Recycling the DES without further purification led to increased
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Recent trends in multidimensional countercurrent chromatography J. Sep. Sci. (IF 3.1) Pub Date : 2024-02-14 Jules Muhire, Xiao Sun, Fu-Xin Zhang, Xin-Yi Huang, Duo-Long Di
Countercurrent chromatography (CCC) is a potent separation approach known for its remarkable efficiency and capacity in preparation. It's applied as a substitute or combined with different chromatographic techniques, resulting in its rebranding as multidimensional CCC (MDCCC). Numerous essential mixtures from natural products contain hundreds or thousands of distinct components of importance. These
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Efficient discovery of active isolates from Dioscorea spongiosa by the combination of bioassay-guided macroporous resin column chromatography and high-speed counter-current chromatography J. Sep. Sci. (IF 3.1) Pub Date : 2024-02-14 Zhenqiang Wang, Jinqian Yu, Lei Zhao, Tong Niu, Xiao Wang
In the present study, twelve compounds from Dioscorea spongiosa were successfully purified by an efficient technique combined bioassay-guided fractionation macroporous resin column chromatography (MRCC) pretreatment and high-speed counter-current chromatography (HSCCC) separation for the first time. Then, D101 MRCC was used to fractionate the crude extract into five parts, which further applied the
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Combination of click chemistry and Schiff base reaction: Post-synthesis of covalent organic frameworks as an immobilized metal ion affinity chromatography platform for efficient capture of global phosphopeptides in serum with chronic obstructive pulmonary disease J. Sep. Sci. (IF 3.1) Pub Date : 2024-02-14 Qian Xu, Xiaoli Guo, Simeng Wang, Quanshou Feng, Shi Yan, Yinghua Yan
Reasonable design and construction of functionalized materials are of great importance for the enrichment of global phosphopeptides. In this work, Ti4+ functionalized hydrophilic covalent organic frameworks by introducing glutathione (GSH) and 2,3,4-trihydroxy benzaldehyde (THBA) via click chemistry and Schiff base reaction (COF-V@GSH-THBA-Ti4+) was constructed and applied for selective enrichment
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Comparison of filter membranes in the analysis of 183 veterinary and other drugs by liquid chromatography-tandem mass spectrometry J. Sep. Sci. (IF 3.1) Pub Date : 2024-02-14 Nicolás Michlig, Steven J. Lehotay, Alan R. Lightfield
Although filtration is one of the most common steps in sample preparation for chemical analysis, filter membrane materials can leach contaminants and/or retain some analytes in the filtered solutions. In multiclass, multiresidue analysis of veterinary drugs, it is challenging to find one type of filter membrane that does not retain at least some of the analytes before injection in ultrahigh-performance
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Optimization of generic conditions for electromembrane extraction of basic substances of moderate or low polarity J. Sep. Sci. (IF 3.1) Pub Date : 2024-02-14 Chenchen Song, Chen Zhou, Frederik André Hansen, Anne Oldeide Hay, Stig Pedersen-Bjergaard
Generic electromembrane extraction (EME) methods were developed and optimized for basic analytes of moderate or low polarity, employing prototype conductive vial EME equipment. Two generic methods, B1 and B2, were devised for mono- and dibasic compounds with distinct polarity windows: 2.0 < log P < 6.0 for B1 and 1.0 < log P < 4.5 for B2. In B1, 10 μL of 2-nitrophenyl octyl ether served as the liquid
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Rapid indirect separation of glutamine enantiomers by micellar electrokinetic chromatography. Analysis of dietary supplements J. Sep. Sci. (IF 3.1) Pub Date : 2024-02-14 Sandra Salido-Fortuna, Paloma Ruano-Culebras, María Luisa Marina, María Castro-Puyana
Glutamine is the most abundant free proteinogenic α-amino acid. It is naturally produced in the organism and acts as a precursor for the synthesis of different biologically important molecules (such as proteins or nucleotides). However, under stressful conditions, the organism is unable to produce it in enough amounts to function properly. Thus, glutamine (Gln)-based supplements have become increasingly
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Comparison of the applicability of electromembrane extraction and liquid-phase microextraction for extraction of non-polar basic drugs from different biological samples: Using clozapine as the model analyte J. Sep. Sci. (IF 3.1) Pub Date : 2024-02-14 Jianhua Xiong, Linxin Tian, Xiantao Shen, Chuixiu Huang
Understanding and comparing the applicability of electromembrane extraction (EME) and liquid-phase microextraction (LPME) is crucial for selecting an appropriate microextraction approach. In this work, EME and LPME based on supported liquid membranes were compared using biological samples, including whole blood, urine, saliva, and liver tissue. After optimization, efficient EME and LPME of clozapine
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Analysis of cerebrospinal fluid metabolites affected by WenDanTang based on ultra-high-performance liquid chromatography coupled with high-resolution mass spectrometry J. Sep. Sci. (IF 3.1) Pub Date : 2024-01-28 Yun Zou, Saixue Tang, Haozhi Li, Feilong Lu, Linlin Shao
WenDanTang (WDT) is a Chinese herbal formula used to treat various diseases, including neurodegenerative diseases. However, the neuroprotective metabolic pathways and the components involved in this process are not fully understood. In this study, we examined the neuroprotective metabolic pathways of WDT in rat brains using cerebrospinal fluid metabolomics and ultra-high-performance liquid chromat
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High temperature-size exclusion chromatography with triple detection to assess the molecular architecture of low-density polyethylene: Insight into branching and processability correlations J. Sep. Sci. (IF 3.1) Pub Date : 2024-01-28 Yogesha Subbaiah, Senthilkumar Shanmugam, Qua Eng Hau
In this study, three commercially available low-density polyethylene (LDPE) polymers produced via a tubular reactor process, with varying melt flow rates at 190°C/2.16 kg (4.0, 1.9, and 0.75 g/10 min), have been selected and subjected to high temperature-size exclusion chromatography (SEC) analysis coupled with an infrared-5 (IR-5), viscometer (VISCO), and multiangle laser light-scattering detectors
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Multilayer coatings based on gold nanoparticles and polymers with bovine serum albumin as a functional layer for the chiral separation in capillary electrochromatography J. Sep. Sci. (IF 3.1) Pub Date : 2024-01-28 Daria Makeeva, Violetta Morgacheva, Ekaterina Kolobova, Elena Solovyeva, Liudmila Kartsova
In this study, we developed physically adsorbed multi-layer coatings using poly-l-lysine or poly(diallyldimethylammonium chloride) and gold nanoparticles, which were functionalized with bovine serum albumin for the chiral separation in electrochromatography. The approach involves sequentially depositing positively charged polymers and negatively charged citrate-stabilized gold nanoparticles. By repeating
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Ultra-high-performance liquid chromatography with tandem mass spectrometry method for cellular toxicity and pharmacokinetic study of PEG1K polymers J. Sep. Sci. (IF 3.1) Pub Date : 2024-01-28 Yuncheng Ge, Ning Zhang, Xinyue Zheng, Luyao Yu, Yajun Liu, Hongyu Xue, Jing Zhao, Jiansong You, Meiyun Shi, Lei Yin
Polyethylene glycol (PEG) is one of the most commonly used polymers in drug delivery systems. The investigation of the pharmacokinetic behavior of PEG is important for revealing the toxicity and efficiency of PEG-related Nano-drug delivery systems. A high through-put and selective ultra-high-performance liquid chromatography with tandem mass spectrometry (UHPLC-MS/MS) method coupled with collision-induced
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In situ growth of imine-based covalent organic framework as stationary phase for open-tubular capillary electrochromatographic separation J. Sep. Sci. (IF 3.1) Pub Date : 2024-01-28 Xiao Niu, Shengda Qi, Jianong Sun, Ailing Zhu, Fangling Wang, Mingfang Wu, Wenjuan Lv, Hongli Chen
Designing advanced stationary phases to improve separation efficiency is essential in capillary electrochromatography. Due to their outstanding performance, covalent organic frameworks have recently demonstrated considerable promise in the field of separation science. Herein, an open-tubular capillary electrochromatography method was reported using porous imine-based covalent organic framework with
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Rapid identification of the compounds of Bushen Huoxue Prescription based on offline two-dimensional liquid chromatography with high-resolution mass spectrometry and molecular network technology J. Sep. Sci. (IF 3.1) Pub Date : 2024-01-28 Wei Kuang, Yu Wang, Yuxia Huang, Wenlin Wu, Mei Zhang
The comprehensive and efficient characterization of components in traditional Chinese medicine is crucial for elucidating its active constituents and uncovering its mechanism. Identifying the compounds of the Bushen Huoxue Prescription (BHP) is difficult because of its complex composition and the large difference in concentration among its compounds. In this study, a hydrophilic interaction liquid
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An optimization strategy for charged aerosol detection to linearize the detector response in the multicomponent quantitative analysis of Qishen Yiqi dripping pills J. Sep. Sci. (IF 3.1) Pub Date : 2024-01-28 Linlin Wu, Xingchu Gong, Haibin Qu
Charged aerosol detection, increasingly recognized for quantifying pharmaceutical compounds with weak ultraviolet absorption, is a universal detection technique for high-performance liquid chromatography (HPLC). Charged aerosol detection shows a non-linear response with increasing analyte concentration over a wide range, limiting its versatility in various analytical applications. In this work, a co-optimization
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Metabolic profiles of Fufang Xiling Jiedu capsule in rats by ultra-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry J. Sep. Sci. (IF 3.1) Pub Date : 2024-01-28 Yu-kang Liu, Ce-jia Liu, Ru-fang Tian, Xin-cun Liu, Yu-wei Zhang, Feng-chao Zhang, Jing-hua Zhang, Yuan-cheng Yao, Gui-yun Cao, Zhao-qing Meng
Fufang Xiling Jiedu capsule (FXJC), a traditional Chinese medicine that evolved from “Yinqiao Powder”, is widely used for the treatment of cold and influenza. However, due to a lack of in vivo metabolism research, the chemical components responsible for the therapeutic effects still remain unclear. Hence, this study aimed to describe the metabolic profiles of the FXJC in rat plasma, urine, and feces
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Green micellar liquid chromatographic method for simultaneous determination of antihistaminic drugs tripelennamine hydrochloride and diphenhydramine in pharmaceutical gel J. Sep. Sci. (IF 3.1) Pub Date : 2024-01-28 Sherin F. Hammad, Samah F. El-Malla, Basma Z. El-Khateeb
A new green micellar liquid chromatographic method has been developed and validated for the simultaneous determination of diphenhydramine (DPH) and tripelennamine hydrochloride (TRP) using a micellar mobile phase consisting of 1 mM Tween 20 in phosphate buffer pH 4:isopropanol (85:15, %v/v). The method was linear in the range of 4–150 and 5–120 μg/mL for TRP and DPH, respectively. The method was successfully
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Evaluation of the effects of three different processing methods of aconite on rat metabolites based on high-coverage pseudotargeted metabolomics J. Sep. Sci. (IF 3.1) Pub Date : 2024-01-11 Huifei Wu, Wenxia Zhang, ZhiEn Wang, Hui Lin, Jiayin Guo, Shijian Quan
Aconite is the processed product of the seed root of Aconitum carmichaelii Debx. Aconite is a commonly used traditional Chinese medicine, which is generally used after processing. Black aconite, light aconite, and salted aconite are three different processed aconite products. They have the effects of restoring yang and saving energy enemy, dispersing cold, and relieving pain. However, clinical aconite
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A novel method for evaluating pseudoallergy based on β-hexosaminidase and its application for traditional Chinese medicine injections J. Sep. Sci. (IF 3.1) Pub Date : 2024-01-11 Chen Yang, Jing-Wen Zhang, Jin Qi, Bo-Yang Yu
Pseudoallergy is a typical and common adverse drug reaction to injections, especially in traditional Chinese medicine injections (TCMIs). At present, the evaluation methods for pseudoallergy include cell methods in vitro and animal methods in vivo. The mast cell evaluation method based on the β-hexosaminidase (β-Hex)-catalyzed substrate, 4-nitrophenyl-β-N-acetyl-D-glucosaminide (4-NPG), is an important
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Enantioseparation of agrochemicals by gas chromatography. Exploring columns based on cyclodextrin derivatives dissolved into polysiloxanes J. Sep. Sci. (IF 3.1) Pub Date : 2024-01-11 Emilia Frickel Critto, Sol Giovannoni, Carlina Lancioni, Cecilia B. Castells
The main goal of this work is to expand the availability of chiral columns for the analysis of agrochemicals by gas chromatography. A broader offer of chiral stationary phases would allow shifting toward enantioselective analytical techniques environmentally more friendly for those compounds. We prepared seven chiral capillary columns based on derivatives of either, β-cyclodextrin or γ-cyclodextrins
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Hydroxyl-rich ferrofluid for efficient liquid phase microextraction of cinnamic acid derivatives in traditional Chinese medicine J. Sep. Sci. (IF 3.1) Pub Date : 2024-01-11 Runqin Wang, Xueling Zhang, Xiangyu Meng, Li Yang, Rongrong Xing, Xuan Chen, Shuang Hu
In this study, a hydroxyl-rich ferrofluid was prepared by dispersing silica-coated magnetic nanoparticles into a methyltrioctylammonium chloride-glycerol deep eutectic solvent and then employed in the preconcentration of trace-level of cinnamic acid derivatives (caffeic acid, p-hydroxycinnamic acid, ferulic acid, and cinnamic acid) in traditional Chinese medicine prior to high-performance liquid chromatography
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Simultaneous determination of sinomenine and its metabolites desmethyl-sinomenine and sinomenine N-oxide in rat plasma by liquid chromatography tandem mass spectrometry J. Sep. Sci. (IF 3.1) Pub Date : 2024-01-11 Ning He, Ali Fan, Dongmei Wu, Hongyan Yu, Kun Wang, Weijie Jiao, Xu Zhao
Sinomenine is an active ingredient extracted from herb medicine, which has been prescribed to treat rheumatoid arthritis in clinics. The present work was to develop a simple method to simultaneously determine sinomenine and its metabolites desmethyl sinomenine and sinomenine N-oxide in rat plasma by liquid chromatography tandem mass spectrometry. Precursor-to-product transitions for detection were
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Integrated ultra-high-performance liquid chromatography coupled with quadrupole-orbitrap mass spectrometry-based components analysis and network pharmacology strategy of Gancao Xiexin Decoction in treating gastric ulcer J. Sep. Sci. (IF 3.1) Pub Date : 2024-01-11 Rongjin Wang, Shoufang Tang, Limei Huang, Ziyi Chen, Yuwen Li, Shu Liu, Fengrui Song, Lihui Men, Zhongying Liu
Gancao Xiexin Decoction (GCXXD) is a traditional Chinese decoction that is often used in treating gastric ulcers. However, the substance basis and mechanism of action remain unclear. In this study, in vivo and in vitro components of GCXXD were analyzed by ultra-high-performance liquid chromatography coupled with quadrupole-orbitrap mass spectrometry. The compound Discover platform was used to ultimately
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Comparison of different extraction methods of active ingredients of Chinese medicine and natural products J. Sep. Sci. (IF 3.1) Pub Date : 2024-01-11 Geyuan Li, Dongya Chen
Like other traditional medicine in the world, Chinese traditional medicine (CTM) has a long history, which is a treasure of the combination of medicine and Chinese classical culture even more than 5000 years. For thousands of years, CTM has made great contributions to the reproduction and health of the Chinese people. It was an efficient therapeutic tool under the guidance of Chinese traditional medical
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Metabolomics-based pharmaceutical evaluation of different parts of Swertia chirayita (Roxb.) Buch.-Ham. ex C.B. Clarke from the western Himalayas J. Sep. Sci. (IF 3.1) Pub Date : 2024-01-11 Devesh Tewari, Sweta Bawari, Saurabh T Mishra, Pawan Gupta, Aryalaxmi M, Zoltán Cziáky, József Jeko, Irina Lazarova, Gökhan Zengin
Swertia species are common ingredients in numerous herbal remedies. It is also used to treat a wide range of illnesses and possess diverse therapeutic activities. The aim of the study is to elucidate the comprehensive metabolomics profile of Swertia chirayita and the role of various extraction methods in the phytochemical compositions of the extracts of S. chirayita, and their antioxidant and enzyme
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Target-guided isolation and purification of cyclooxygenase-2 inhibitors from Meconopsis integrifolia (Maxim.) Franch. by high-speed counter-current chromatography combined with ultrafiltration liquid chromatography J. Sep. Sci. (IF 3.1) Pub Date : 2024-01-11 Aijing Li, Mencuo La, Yao Wang, Tao Chen, Ruisha Mian, Fangfang He, Yulin Li, Denglang Zou
Meconopsis integrifolia (Maxim.) Franch. is used extensively in traditional Tibetan medicine for its potent anti-inflammatory properties. In this study, six cyclooxygenase-2 (COX-2) inhibitors were purified from M. integrifolia using high-speed counter-current chromatography guided by ultrafiltration liquid chromatography (ultrafiltration-LC). First, ultrafiltration-LC was performed to profile the
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Development and validation of a sensitive liquid chromatography-tandem mass spectrometry method for the assay of 12 substances in rat plasma and its application to rat pharmacokinetics of Epimedium and Psoraleae Fructus herb pair after oral administration J. Sep. Sci. (IF 3.1) Pub Date : 2024-01-11 Linwei Wang, Xiaoying Yin, Huan Liu, Yangyang Wang, Zhixiong Li, Yuxuan Zhao, Haibo Xu, Chenggang Huang, Xingxing Diao
Epimedium (EM) and Psoraleae Fructus (PF) are a traditional herb combination often used as a fixed form to treat osteoporosis disease in the clinic. However, the intricate interactions of this pair remain unknown. In our study, we undertook a comprehensive examination of their compatibility behaviors. Concurrently, a precise and sensitive quantitation method was successfully developed and validated
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Analysis of 14 β-agonists in pork using an automated wooden tip-based solid-phase microextraction device and ultra-high-performance liquid chromatography–tandem mass spectrometry J. Sep. Sci. (IF 3.1) Pub Date : 2024-01-11 Kai Li, Xiaoming Gong, Liming Jin, Mengxiao Liu, Mengmeng Hua
This study introduces a cost-effective, automated ultra-high-performance liquid chromatography–tandem mass spectrometry method for the detection of 14 β-agonists in pork using a novel solid-phase microextraction probe composed of polyacrylonitrile and molecularly imprinted polymer. Integrated into an automated extraction device, the probe optimizes extraction prior to analysis while reducing expenses
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Contactless conductivity detector as a tool for improving universality and sensitivity of capillary electrophoresis-frontal analysis: Proof of concept J. Sep. Sci. (IF 3.1) Pub Date : 2024-01-11 Taťána Bržezická, Hana Mlčochová, Zdeněk Glatz, Lenka Kohútová
Drug binding to plasma proteins influences processes such as liberation, adsorption, disposition, metabolism, and elimination of drugs, which are thus one of the key steps of a new drug development. As a result, the characterization of drug–protein interactions is an essential part of these time- and money-consuming processes. It is important to determine not only the binding strength and the stoichiometry
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Chemical composition and absorption characteristics of Raw and Prepared Cassiae Semen extracts based on ultra-high-performance liquid chromatography-quadrupole Orbitrap high-resolution mass spectrometry J. Sep. Sci. (IF 3.1) Pub Date : 2024-01-11 Yingying Chen, Hongwei Wu, Lixia Wang, Chunyu Yue, Xiaoxu Chen, Tong Wu, Yang Yang, Liying Tang, Zhuju Wang
In traditional Chinese medicine, the two commodity forms of Cassiae Semen Raw and Prepared Cassiae Semen, exert different clinical applications, in which Prepared Cassiae Semen is commonly used to treat liver and eye diseases. However, the material basis of Raw and Prepared Cassiae Semen remains unclear due to the limited studies on their overall composition and metabolism in vivo. In this study, an
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Chemical profiling of Simiao pill and quantification of main effective constituents in it by ultra-high-performance liquid chromatography coupled with Q Exactive Orbitrap and triple quadrupole mass spectrometry J. Sep. Sci. (IF 3.1) Pub Date : 2024-01-11 Le Yang, Ye Sun, Shuyun Wei, Hao Wen, Ruicheng Liu, Xijun Wang
Simiao pill is one of the most commonly used prescriptions in traditional Chinese medicine for the treatment of hyperuricemia and gout. However, methods based on more accurate and comprehensive qualitative and quantitative analyses of the active ingredients are not yet perfect due to limited methodology. This not only hinders the elucidation of the pharmacological mechanism of Simiao pill, but also
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Simultaneous chromatographic separation of the anomers of saccharides on a polymer sulfobetaine zwitterionic stationary phase J. Sep. Sci. (IF 3.1) Pub Date : 2023-12-24 Min Yang, Haokun Mei, Yu Jiang, Feifang Zhang, Ziteng Yu, Bingcheng Yang
Simultaneous chromatographic separation of the anomers of saccharides was achieved by using a polymer zwitterionic stationary phase functionalized by acrylamide-type sulfobetaine. By optimization of separation parameters including column temperature, pH, and flow rate, the column operated in hydrophilic interaction chromatography mode exhibited excellent separation selectivity toward five monosaccharides
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Deep eutectic solvent extraction combined with magnetic bead ligand fishing for identification of α-glucosidase inhibitors from Pueraria lobata J. Sep. Sci. (IF 3.1) Pub Date : 2023-12-22 Bing Li, Pan Jiao, Cheng Tang
In this study, a deep eutectic solvent (DES) extraction combined with a magnetic bead ligand affinity analytical method was developed and used for α-glucosidase inhibitor identification from Pueraria lobata. Several critical parameters affecting the analysis performance, including the type of DES, molar ratio, water amount, pH, salt concentration, and volume of DES, were investigated. The selected
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A quality-by-design evaluated liquid chromatography method development and validation for the separation and quantification of nitroxoline and its impurities J. Sep. Sci. (IF 3.1) Pub Date : 2023-12-22 Santhi Priya Lankalapalli, K. Vijaya Rachel, Vaishnavi Chintala, Leela Prasad Kowtharapu, Naresh Kumar Katari
A novel, isocratic, sensitive, stability-indicating high-performance liquid chromatography method was developed for the separation and quantification of related substances in nitroxoline (NTL). The chromatographic separation has been achieved on Inertsil ODS-3 V, (250 × 4.6 mm, 5 μm) at 240 nm using ethylenediamine tetraacetic acid buffer and methanol in the ratio of 60:40 v/v as mobile phase. The
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Screening, isolation, and activity evaluation of potential xanthine oxidase inhibitors in Poria Cum Radix Pini and mechanism of action in the treatment of gout disease J. Sep. Sci. (IF 3.1) Pub Date : 2023-12-22 Siyuan Zhuang, Haocheng Yun, Xu Zhou, Yanjie Li, Sainan Li, Chunming Liu, Yuchi Zhang
Poria Cum Radix Pini is a rare medicinal fungus that contains several potential therapeutic ingredients. On this basis, a particle swarm mathematical model was used to optimize the extraction process of total triterpenes from P. Cum Radix Pini, and xanthine oxidase inhibitors were screened using affinity ultrafiltration mass spectrometry. Meanwhile, the accuracy of the ultrafiltration assay was verified
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A robust method for simultaneous determination of eight B vitamins in human serum by liquid chromatography tandem mass spectrometry J. Sep. Sci. (IF 3.1) Pub Date : 2023-12-20 Yu-Mei Huo, Shang-Qing Zhang, Gao-Ping Wu, Hong-Bo Shan, Chao Pan
The level of vitamin B group in human serum is an important index of human health. Among B vitamins, cyanocobalamin in serum is unstable and its content is extremely low. Rapid and simultaneous detection of multiple B vitamins including cyanocobalamin is a challenge. Herein, we have developed a rapid and stable method that can realize the determination of thiamine, riboflavin, nicotinamide, pantothenic
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Large-scale separation of alkaloids from Corydalis decumbens by pH-zone-refining centrifugal partition chromatography and their anticomplement activity J. Sep. Sci. (IF 3.1) Pub Date : 2023-12-18 Xin-Xin Xie, Zhong-Jian Chen, Quan-Gang Zhu, Qin Yu, Tian-Yan Lian, Xue-Lian Xu, Ya Chen, Wei-Hua Song
Centrifugal partition chromatography in the pH-zone-refining mode was successfully applied to the separation of alkaloids from the crude extract of Corydalis decumbens. The experiment was performed with a two-phase solvent system composed of petroleum ether-ethyl acetate-ethanol-water (5:5:3:7, v/v/v/v) where triethylamine (10 mM) was added to the stationary phase and hydrochloric acid (10 mM) to the
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Characterization and quality evaluation of QiXueShuFu Decoction based on fingerprint and ultra-performance liquid chromatography-quadrupole-orbitrap mass spectrometry J. Sep. Sci. (IF 3.1) Pub Date : 2023-12-14 Liang Yao, Yuzhen Liu, Yu Cui, Julin Sun, Xiaojian Xia, Junping Wang, Yougang Wei, WeiDong Chen
QiXueShuFu Decoction (QXSFD) modified from the Bazhen Decoction which was originally from the classic Ming Dynasty is a traditional folk formula that boosts the body's immune system. However, its ambiguous chemical components limited its quality control evaluation. In this study, ultra-performance liquid chromatography (UPLC) fingerprint combined with multivariate analysis was used to evaluate the
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Rapid screening and evaluation of natural antioxidants from leaf, stem, and root of Artemisia argyi by online liquid microextraction combined with HPLC-based antioxidant assay system coupled with calibration quantitative analysis J. Sep. Sci. (IF 3.1) Pub Date : 2023-12-14 Wei-Qi Yang, Qi Huang, Meng-Qi Wu, Quan-Xi Mei, Yuan-Sheng Zou, Zheng-Ming Qian, Dan Tang
To reveal the utilization value of leaf, stem, and root of Artemisia argyi, a rapid online liquid microextraction combined with a high-performance liquid chromatography coupled with 2,2-nitrogen-di (3-ethyl-benzothiazole-6-sulfonic acid) diammonium salt antioxidant assay system was established for analysis of antioxidants in the leaf, stem, and root of A. argyi, and a calibration quantitative method
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Evaluation of solid-phase extraction sorbents for purification of oligosaccharides and glycans derivatized by positively charged labels followed by capillary electrophoretic analysis J. Sep. Sci. (IF 3.1) Pub Date : 2023-12-14 Denisa Smolkova, Dana Moravcova, Jana Lavicka
The sample preparation including labeling and clean-up represents a key analytical step in the analysis of oligosaccharides and glycans by either chromatographic or electrophoretic separation methods. Although the majority of labeling has been performed by neutral and/or negatively charged tags, the introduction of a positive charge into the saccharide molecule can significantly improve the analysis