Dark teas are prepared by a microbial fermentation process. Flavan-3-ol B-ring fission analogues (FBRFAs) are some of the key bioactive constituents that characterise dark teas. The precursors and the synthetic mechanism involved in the formation of FBRFAs are not known. Using a unique solid-state fermentation system with β-cyclodextrin inclusion complexation, as well as targeted chromatographic isolation

Cobalamin-independent methionine synthases (MS) are zinc-binding methyltransferases that catalyze de novo methionine biosynthesis in higher plants, which are enzymes critically involved in seed germination and plant growth. Here we report a highly selective sulfonyl fluoride-based probe for chemoproteomic profiling of MS enzymes in living systems of the model plant Arabidopsis thaliana, as implemented

The sensory impacts of two thiols, 2-methyl-3-furanthiol (MFT) and 2-furfurylthiol (FFT), in Chinese soy sauce aroma-type Baijiu (SSB), strong aroma-type Baijiu (STB), and light (LTB) aroma-type Baijiu (liquor) were evaluated and combined with partial least squares discriminant analysis (PLS-DA) to differentiate Chinese Baijiu. The flavor dilution (FD) factors of these two thiols ranged from 81-6561

ABSTRACT: Rapeseed (Brassica napus L.) is highly susceptible to boron (B) and phosphorus (P) deficiencies, yet knowledge of how these two essential elements interact to contribute to plant growth and crop yield is limited. To this end, a pot experiment with three P application rates (5, 75 and 150 mg P2O5 kg-1 dry soil) and two B application rates (0.25 and 1 mg B kg-1 dry soil) was conducted. The

The increased use of the emerging per- and polyfluoroalkyl substances (PFASs) has led to extensive concerns around the world. Effective detection methods to trace their pollution characteristics and environmental behaviors in complex soil-crop systems are urgently needed. In this study, a reliable and matrix effect-free method was developed for simultaneous determination of fourteen legacy and emerging

In the context of the expansion of the human population, availability of food, and in extension of animal feed, is a big issue. Favoring a circular economy by the valorisation of by-products is a sustainable way to be more efficient. Animal by-products are an interesting source of feed materials due to their richness in proteins of high nutritional value. Prevention and control efforts have allowed

Typical glycoconjugate of glutamic acid, namely, N-(β-D-deoxyfructos-1-yl)-L-glutamic acid was successfully synthesized as the primary isomer in yield of 96.08% using a food-grade preparation method, and its chemical structure was clearly demonstrated by mass spectrometry and 1D/2D NMR. The reaction kinetics of glucose and glutamic acid were systematically studied to investigate the effect of lyophilization

The production of acetone-butanol-ethanol by solventogenic Clostridium using lignocellulosic biomass can be a potential alternative to petroleum-based butanol. However, previous studies on non-detoxified lignocellulose hydrolysate could not provide better results when compared to those in synthetic medium. In this study, we engineered the pentose pathway of Clostridium beijerinckii NCIMB 8052, which

RNA interference is a promising crop protection technology that has seen rapid development in the past several years. Here, we have investigated polyamino acid biopolymers, inorganic nanomaterials, and hybrid organic-inorganic nanomaterials for delivery of dsRNA and efficacy of gene knockdown using the model nematode Caenorhabditis elegans. By using an oral route of delivery, we are able to approximate

The peel of Citrus reticulata “Chachi” (GCP), which is highly valued in China for its health-promoting effects, is usually collected at different development stages to be processed into various functional foods. In the present work, a rapid method based on UHPLC-QE Orbitrap MS incorporated untargeted and pseudotargeted metabolomics analysis was developed to investigate the chemical variations in GCP

Soaking of soybean seeds is prerequisite for the production of soy foods and it has been shown that the extent of water absorbed during different imbibition conditions directly affects the quality of the subsequent soybean seed products by yet unknown mechanisms. In order to elucidate the molecular changes in soybean seeds during different soaking temperatures, we performed an integrated proteomics

This work aimed to investigate the effect of enzymatic crosslinking on the allergenic potential of shrimp tropomyosin (TM), Met e 1. The crosslinked TM with laccase (CL), laccase/caffeic acid (CLC and CLC+) and transglutaminase (CTG and CTG+) formed macromolecules and altered the allergen conformation. The IgG/IgE-binding potentials of the crosslinked TM were reduced as confirmed by western blotting

Enzymatic browning is one of the main problems faced by the food industry due to the enzyme polyphenol oxidase (PPO) action provoking undesirable colour change in the presence of oxygen. Here, we report the evaluation of ten different azamacrocyclic compounds with diverse morphologies as potential inhibitors against the activity of PPO, both in model and real systems. An initial screening of ten ligands

The non-anthocyanin phenolic compounds in juice and wine produced from fruits of white bilberry, a non-pigmented mutant of Vaccinium myrtillus, and blue bilberry (pigmented variety) were analyzed using LC–DAD and LC–DAD–ESI-QTOF-MS. On the basis elution order, UV-vis spectra, accurate mass data and fragmentation pattern and standards, 42 compounds, including 22 phenolic acids, 15 flavonols, and 5 flavan-3-ols

Proteus mirabilis could convert L-phenylalanine into 2-phenylethanol via the Ehrlich pathway, the amino acid deaminase pathway, and the aromatic amino acid decarboxylase pathway. The aromatic amino acid decarboxylase pathway was proved for the first time in P. mirabilis. In this pathway, L-aromatic amino acid transferase demonstrated a unique catalytic property, transforming 2-penylethylamine into

The following joint Editorial was originally published in ACS Applied Materials & Interfaces (DOI: 10.1021/acsami.0c10979). We confront the terrible reality that systemic racism and discrimination impacts the daily personal and professional lives of many members of the scientific community and broader society. In the U.S., the brutal killing of George Floyd while in police custody is one of the most

To decrease D-lactic acid production cost, cane molasses and soybean meal, low-cost agro-industrial wastes, were selected as feedstock. Firstly, cane molasses was used directly by L. delbrueckii S-NL31, and the nutrients were released from soybean meal by protease hydrolysis. Subsequently, to ensure intensive substrate utilization and enhanced D-lactic acid production from cane molasses and soybean

Lipophenols have been stressed as an emerging class of functional compounds. However, little is known about their diversity. Thus, this study is aimed at developing a new method for the extraction, clean-up, and UHPLC-ESI-QqQ-MS/MS-based analysis of the lipophenols derived from hydroxytyrosol (HT): α-linolenic (HT-ALA), linoleic acid (HT-LA), and oleic acid (HT-OA). The method validated provides reliable

The onion, known as the bulb onion or common onion, is not only key ingredients in many tasty and healthy vegetarian meals but also many traditional medicines. Nine new flavonoids [cepaflavas A, B (5, 6), cepadials A–D (7–9, and 14), and cepabiflas A–C (10–12)] and six known compounds (1–4, 13, 15) were obtained from the outer skins of Allium cepa L.. Among them, compounds 5, 6, and 9 might be artificial

Fosthiazate is a widely used chiral organophosphorous nematicide with four stereoisomers. The present study systemically assessed the stereoselectivity of fosthiazate for the first time, including absolute configuration confirmation, stereoselective bioactivity toward nematode and aphid, toxicity to honeybees, and the stereoselective degradation in cucumber and pepper under field conditions. The absolute

The chemical structure of β-carotene in Figure 8 and the TOC graphic was drawn in error in the original publication and is corrected here. Figure 8. Formation of β-ionone in tea (C. sinensis) leaves exposed to multiple stresses during the tea withering process. TOC graphic In addtion, the GenBank numbers were listed in error and corrected here. GenBank accession numbers MH119136 (CsCCD1a), TEA013266

The kinetics of the reaction between epicatechin and various carbonyl compounds typically formed in cooked and stored foods were evaluated in model systems at pH 7.4 and 37 °C, and the corresponding reaction products in stored UHT milk added epicatechin were identified by HPLC-MS/MS. The rate constants for the reactions of carbonyl compounds with epicatechin decreased in the following the order: methylglyoxal;

Microcystins (MCs) are hepatotoxic heptapeptides produced by cyanobacteria and are potent inhibitors of protein phosphatases in eukaryotic cells. Algae for dietary supplements are harvested from outdoor environments and can be contaminated with MCs. Monitoring of MCs in these products is necessary, but is complicated by their structural diversity (> 250 congeners). We used a combination of protein