Signal amplification plays a significant role in nucleic acid analysis, especially in trace nucleic acid detection. Transcription-based signal amplification is a very important component in the field of signal amplification, and has received extensive attention depending on outstanding cyclic transcription ability. However, the low efficiency of transcription caused by limited template length usually

Arsenic(III) is a highly toxic pollutant in the environment, and the development of nanomaterials with high electrochemical activity for As(III) detection is a research focus at present. Herein, an electrochemical sensing platform based on polyaniline nanofibers embedded with Ag@SiO2 nanoparticles (Ag@SiO2/PANI NFs) was successfully constructed for trace As(III) determination. Owing to an in-situ chemical

The localization of photosensitizers and timely efficacy evaluation highly affect efficiency of photodynamic therapy (PDT) and the resistance of residual tumor cells. Mitochondria-targeted PDT is an effective modality; however, it may encounter certain resistance caused by autophagy, a cytoprotective manner competing with cell apoptosis. Mitochondria and RNA interact closely in cell apoptosis and autophagy

Extracellular antibiotic resistance genes (eARGs) exacerbate the propagation of antimicrobial resistance in the environment and pose great threats to human health. On-site identification and quantification of multiple eARGs at trace level are an urgent need. Herein, we designed a core-satellite platform based on surface enhanced Raman scattering (SERS) to simultaneously detect multiple eARGs (sul1

In this work, we demonstrate a novel cascaded fiber-optic structure depending on Fabry-Perot interference and anti-resonance (AR) effect for seawater salinity and temperature simultaneous measurement. An in-fiber microchannel Fabry-Perot interferometer (FPI) is obtained in a single-mode fiber (SMF) by using femtosecond laser radiation and hydrofluoric acid corrosion, which is filled with thermosensitive

Volatile organic compounds (VOCs) detection in ultra-low concentrations is highly demanded in industry and daily life. Ethanol is one of the most encountered VOCs. Through calcining the precursor of metal-organic frameworks (MOFs), which are in situ grown on 2D Ti3C2Tx sheets, Co3O4/Ti3C2Tx nanocomposites with the mesoporous structure were synthesized for ethanol sensing. The results showed that the

In this work, cost-effective and highly sensitive electrochemical magnetic molecularly imprinted particles (MMIPs) sensors were proposed for indirectly detecting sulfamethazine (SMZ) and sulfamonomethoxine (SMM). MMIPs were synthesized using a sol-gel process using SMZ or SMM as a template, Fe3O4-NH2 as a core, and tetraethoxysilane as a cross-linking agent, then the template removal. The electrochemical

The design of the FET-type gas sensor affects various sensor performance factors such as sensitivity, noise, and power consumption. However, few studies comprehensively consider the impact of sensor design on the factors. Here, we show how the design of FET-type gas sensors influences a variety of performance factors and provide design guidelines for FET-type gas sensors. Sensors with several sensing

This paper reports on ammonia (NH3) sensing characteristics of a new semiconducting metal oxide (SMO) sensor. employing a cerium oxide (CeO2) thin film and platinum (Pt) nanoparticles (NPs) as a sensing layer. The thickness of CeO2 thin film is 30 nm and the particle size of Pt NPs is about 4 to 6 nm. Based on the enhanced surface area/volume (SA/V) ratio and catalytic ability of Pt metal, the sensing

Piwi-interacting RNAs (piRNAs) are a class of noncoding small RNAs that have been identified as promising biomarkers for cancer detection. However, the development of emerging detection methods for piRNAs has not attracted widespread attention. In this work, we proposed an ultrasensitive photoelectrochemical (PEC) biosensor that combines photosensitive hybrid with enzymatic signal amplification strategy

Protein adsorption onto solid surfaces is an immanent and spontaneous phenomenon that occurs in many fundamental biomedical applications. Recent research mainly focuses on collectively measuring the protein behaviors in confined localized nanosensing areas, whereas ignoring the ambient aqueous variation that directly affects protein behavior, such as the hydrogen bonding network in protein-water which

Photonic crystal fibers (PCFs), providing ingenious microfluidic channels and intense light guidance, have many advantages in the detection of three-dimensional surface-enhanced Raman scattering (SERS). In solid-core PCFs, the light interacts with the sample in the form of an evanescent field, which is weak and limits the SERS detection performance. In this paper, we propose a new mechanism based on

Developing low-cost optical hydrogen sensors for applications in the fields related to hydrogen economy has attracted great attentions, but still remains challenging. Herein, a new type of optical indicator based on Pd-decorated PdO nanoparticle nanonetworks (Pd-PdO NNs) is reported. Pd-PdO NNs have the capability to indicate the presence of hydrogen in concentration range of 0.5% to 100 vol% H2 in

High-field asymmetric waveform ion mobility spectrometry (FAIMS) is a specific ion mobility device used to detect and characterize gas-phase ions under ambient conditions. This device separates ions based on differences in their mobilities, which are characterized by alpha dependence (α(E/N)). However, no effective theoretical model currently exists to describe the accuracy and optimize experimental

In this study, silver nanoparticle-coated polydopamine-copper phosphate hybrid nanoflowers (Ag-PDA-Cu3(PO4)2 NFs) were synthesized and demonstrated to serve as efficient surface-enhanced Raman spectroscopy (SERS) probes for detecting thiol-containing molecules such as benzenethiol and thiocholine. Ag-PDA-Cu3(PO4)2 NFs were fabricated by forming PDA-Cu3(PO4)2 NFs through the nucleation of Cu3(PO4)2

Detection of NO2 attracted increasing interest to check the air quality and monitor exhaust emission of combustion facilities. In this study, the flame spray pyrolysis (FSP) method was used to prepare the La-doped WO3 nanoparticles as NO2 sensing materials for the first time. The FSP-made La-doped WO3 nanoparticles showed that the doped La atoms were dispersed homogeneously on WO3 particles in the

Gas sensors with trivalent cation-conducting solids have been reviewed. In general, because ionic conductivity in solids is strongly influenced by the valence state of migrating ion species, multivalent cation conducting solids are not considered to be suitable candidates for electrochemical devices, including gas sensors. However, the discovery of highly trivalent cation conducting solids in two-

2, 2′-azinobis-3-ethylbenzthiazoline-6-sulfonate (ABTS), 3, 3′, 5, 5′-tetramethylbenzidine (TMB), o-phenylenediamine (OPD) are typical chromogenic substrates to evaluate the activity of peroxidase-like nanozymes. However, these substrates are toxic, unstable, and the coloration reactions are slow. Herein, the classical starch-sodium iodide reagent was utilized as the chromogenic substrate to evaluate

Monitoring trace circulating tumor cells (CTCs) in whole blood is important for early diagnosis and treatment of cancers. Whereas, it remains a major difficulty for detecting CTCs because of their extremely low contents in blood. Herein, by coupling biobarcode amplification method with sequence-responsive and enzyme-embedded DNA nanocapsules, we report a highly sensitive, label-free and specific electrochemical

This study aimed to develop a fluorescent molecularly imprinted photonic crystals hydrogel (FMIPH) strip based on carbon dots (CDs) and inverse opal photonic crystals (PCs) for the detection of the active ingredient rutin in Sophora japonica products. The FMIPH-based sensing system was constructed using rutin as template molecules, 4-vinylpyridine as functional monomers, ethylene glycol dimethacrylate

Carbon dioxide (CO2) sensor has a wide range of applications in many industrial fields. However, most existing CO2 sensors exhibit obvious cross-sensitivity to environmental relative humidity (RH). Therefore, it becomes critical for CO2 sensors to eliminate the interference of humidity. In this study, based on the advantages of conveniently constructing sensing network and multiplexing capability of

In this paper, a gas sensing technique based on multi-resonance photoacoustic spectroscopy (M-PAS) is firstly proposed for simultaneous detection of multi-component gas molecules. To explore the capabilities of this technique, a spherical resonator with multiple resonant modes and high quality factors are detailedly developed by theoretical simulation and experimental analysis. The proposed M-PAS technique

Single cell analysis preserves the heterogeneity information of target cell population in search of rare biomarkers for disease diagnosis. Microfluidic technology facilitates single cell analysis through its high integrability with multi-functionalities, high sensitivity, precision and dynamic range for digital assays. However, demonstrated microfluidic devices for single cell analysis suffer from

As a new generation of electrochemiluminescence (ECL) technology, the electrochemical stripping chemiluminescence (ESCL), which combines the advantages of chemiluminescence and stripping voltammetry, has attracted increasing attention in the biosensor field. Here, Ag nanoclusters (NCs) were prepared using DNA double strands as a template and explored to construct an ESCL aptamer sensor (aptasensor)

A novel ECL biosensor was developed for ultrasensitive mercury ions (Hg2+) determination based on gold nanoparticles-coated nitrogen-doped carbon dots (Au@CNDs) as luminophores and exonuclease III (Exo III) as well as CRISPR/Cas12a as signal amplifiers. The biosensor was fabricated by pre-modifying Au@CNDs hybrids and ferrocene-labeled single-stranded DNA (Fc-ssDNA) layer in sequence on the interface

Accurate, robust, and rapid diagnostics is the basis for all well-functioning healthcare. There is a large need in point-of-care biosensors to facilitate diagnosis and reduce the need for cumbersome laboratory equipment. Proteases are key virulence factors in periodontitis. Periodontal disease is very common and characterized by inflammation and infection in the tooth-supporting structures and is linked

Alzheimer’s disease (AD), as a serious neurodegenerative disease, has posed a great threat to human health. Sensitive detection of its specific biomarker amyloid-β oligomers (AβO) is very important. This work developed a novel electrochemiluminescence (ECL) biosensor for detecting AβO with poly [(9,9-dioctyl-fluorenyl-2,7-diacyl)-alt-co-(9-hexyl-3,6-carbazole)] nanoparticles (PFA NPs) as luminophore

In this work, a novel electrochemiluminescence (ECL) biosensor was fabricated to detect the mutant KRAS gene in the tumor tissues. Firstly, a new kind of chitosan-based dots was prepared and regulated by phenylboronic acid (PBA) as ECL nanoprobe. Furthermore, the electrochemically deposited Ag structures with controllable morphology have been developed on the electrode. The detailed electrochemical

The rapid and accurate determination of Cardiac troponin I (cTnI) has become more and more important to decrease the mortality of acute myocardial infarction (AMI). Herein, signal-on electrochemical aptasensors were fabricated for the sensitive and label-free determination of cTnI by combining the physical and electrochemical properties of ferrocene-based covalent organic framework nanosheets (Fc-COFNs)

Digital microfluidic chips are liquid processors that perform biochemical assays by moving, merging and splitting droplets using the electrowetting effect. Yet, hardwiring electrowetting chips becomes tedious as soon as they include more than a few dozen electrodes. Single-sided continuous opto-electrowetting, where the electrowetting effect of a featureless semiconductor film is controlled by light

Herein, a novel room-temperature methanol sensor was designed and fabricated by constructing a core-shell heterostructure composed of stannous sulfide (SnS) and zeolitic imidazolate framework-8 (ZIF-8). The core-shell morphology of the obtained SnS/ZIF-8 nanocomposite is characterized by tiny ZIF-8 nanocrystalline uniformly attached on the surface of SnS nanoflakes. During the synthesis, the SnS surface

Semiconducting metal oxides and metal catalysts have been widely used for gas sensors with high precision and reliability. In this study, we report a Pd-WO3 based chemiresistive sensor that can detect hydrogen at the ppm level at room temperature with a remarkably high response and selectivity. The colloidal solutions with Pd and WO3 nanoparticles were spray-deposited with controlled charge states

A flexible sensor can be applied to monitor human motion, which can further be engineered with soft robotics to monitor their motion in the real time. However, to realize deformation detection with high-sensitivity in soft robots while simultaneously maintaining a high linearity and cyclic stability of the sensor yet remains as an unmet challenge. Herein, we fabricated strain sensors exhibiting a buckle

Timely and large-scale screening of pathogen-caused infection is highly important to prevent the latent epidemic. With its high amplification efficiency and less requirement on the instrument, the isothermal nucleic acids amplification (INAA) technique has shown great potential in realizing point-of-care (POC) detection of pathogens. However, the ability to realize a high-order multiplexity in one

Cell responses to varying electric fields can reveal insights on cell biology with important implications for pharmaceutical and basic research. In this work, we exploit spectral information content in Opto-Electronic Tweezers (OET) systems through machine learning for label-free characterization of cell dielectric properties aimed at cell classification and drug response evaluation. A customized

As an important antitussive and expectorant herbal medicine, Fritillariae cirrhosae Bulbus (FCB) is rare and expensive, leading to massive adulterations in the market. Herein, we report a solid colorimetric sensor array (CSA) method for the rapid identification of FCB and its adulterations. This CSA was fabricated by printing nine pH indicators on a mixed cellulose ester membrane via a commercially