A feature of severe COVID-19 is the onset of an acute and intense systemic inflammatory response referred to as the “cytokine storm”. The cytokine storm is characterized by high serum levels of inflammatory cytokines and the subsequent transport of inflammatory cells to damaging levels in vital organs (e.g., myocarditis). Immune trafficking and its effect on underlying tissues (e.g., myocardium) are

A conventional molecular assay-based point-of-care (POC) diagnostic test involves three major stages: deoxyribonucleic acid (DNA) extraction, amplification, and amplicon detection. Among these steps, DNA extraction is costly and time-consuming. Nevertheless, it is a crucial step for the identification of sensitive and specific diseases. This review summarizes the advantages and disadvantages of DNA

As a non-invasive, highly specific and sensitive assay strategy, liquid biopsy plays an important role in the diagnosis, the prognosis and the adjuvant therapy of cancer. Circulating tumor cell (CTC) have been considered as a crucial biomarker for liquid biopsy. However, CTCs are difficult to be detected accurately due to their very low content in blood. Herein, HepG2 cells stained with Hcy-716 fluorescence

Electrochemical–electrochemical (EE) redox cycling using interdigitated array (IDA) electrodes is a simple and efficient approach for signal amplification. However, IDA electrodes with high or low electrocatalytic activities are inappropriate for obtaining high signal-to-background ratios in serum containing various interfering redox-active species. Accordingly, herein, Au IDA electrodes are modified

Many microfluidic “lab on paper” devices have been demonstrated for point-of-use applications, but scalable fabrication methods are necessary for these devices to make it past the proof-of-concept stage and become viable commercial products. Commercially available wax printers such as the Xerox ColorQube presented a near-ideal compromise between cost, ease of prototyping, and throughput, but in 2016

Metal oxide-based gas sensors are widely studied and applied due to simple operation principle, small size and high stability. However, numerous gas sensors based on metals oxides usually exhibit high optimal operating temperatures and show poor response/recovery performance at room temperature (RT). To overcome the above problems, innovative device forms should be explored. Herein, we developed a

The rapid and accurate measurement of Raman signals on the test line (T-line) of surface-enhanced Raman scattering immunochromatography assay (SERS-ICA) strip is necessary for on-site detection based on SERS. Here, a novel linear spot Raman detector which can directly collect the SERS signals of the whole T-line on the dual-diagnostic SERS-ICA strip is developed for detection of multiple respiratory

MicroRNAs (miRNAs) are non-coding RNA sequences that regulate many biological processes and have become central targets of biomedical research. However, their naturally low abundances in biological samples necessitates the development of sensitive analytical techniques to conduct routine miRNA measurements in research laboratories. Digital PCR has the potential to meet this need because of its single-molecule

In recent years, magnetic nanoparticle sensor technologies have attracted considerable interest in the point-of-care-testing (POCT) field, especially in Lateral Flow Immunoassays (LFIA). Although the visual signal of magnetic nanoparticles is reduced during inspection, it can be compensated for by magnetic induction, and detection results can be quantified by magnetic sensors. Sensors that use magnetic

Droplet injection strategies are a promising tool to reduce the large amount of sample consumed in serial femtosecond crystallography (SFX) measurements at X-ray free electron lasers (XFELs) with continuous injection approaches. Here, we demonstrate a new modular microfluidic droplet injector (MDI) design that was successfully applied to deliver microcrystals of the human NAD(P)H: Quinone oxidoreductase

Microfluidic impedance cytometry is emerging as a label-free, low-cost and portable solution for cell analysis. Impedance-based cell or particle characterization is provided by microfluidic and electronic devices. We report the design and characterization of a miniaturized flow cytometer based on a 3-dimensional (3D) hydrodynamic focusing mechanism. The sheath adaptively concentrated the sample laterally

In this work, an analytical method for the determination of two endogenous aldehydes (hexanal and heptanal) as lung cancer biomarkers in saliva samples is presented for the first time. The method is based on a modification of magnetic headspace adsorptive microextraction (M–HS–AME) followed by gas chromatography coupled to mass spectrometry (GC-MS). For this purpose, an external magnetic field generated

In this paper, a Pt/Al2O3/AlGaN/GaN metal-insulator-semiconductor high electron mobility transistor (MIS-HEMT) sensor was fabricated for sensing hydrogen (H2) gas and compared to a more conventional Pt-HEMT sensor. The drain-source current (Ids) versus gate-source voltage (Vgs) property (transfer curve) and hydrogen detection characteristics in synthetic air ambient between the HEMT sensor and MIS-HEMT

In this experiment, using MXene quantum dot (MQD) as the core and thiamethoxam (TMX) as the template, a dual-functional surface molecularly imprinted polymer nanoprobe (MQD@MIP) for detecting of TMX was synthesized by microwave digestion procedure. And MQD@MIP was characterized in detail. It was found that this nanoprobe had dual function, which not only could specifically recognize TMX, but also could