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Continuous Flow Delivery System for the Perfusion of Scaffold-based 3D Cultures Lab Chip (IF 6.1) Pub Date : 2024-07-26 Zachary Ryan Sitte, Elizabeth E. Karlsson, Haolin Li, Haibo Zhou, Matthew Ryen Lockett
The paper-based culture platform developed by Whitesides readily incorporates tissue-like structures into laboratories with established workflows that rely on monolayer cultures. Cell-laden hydrogels are deposited in these porous scaffolds with micropipettes; these scaffolds support the thin gel slabs, allowing them to be evaluated individually or stacked into thick constructs. The paper-based culture
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Integration of 3D Printed Mg2+ Potentiometric Sensors into Microfluidic Devices for Bioanalysis Lab Chip (IF 6.1) Pub Date : 2024-07-26 Sarah Farahani, Dalton L Glasco, Manar Elhassan, Pedaballi Sireesha, Jeffrey Bell
Electrochemical sensors provide an affordable and reliable approach towards the detection and monitoring of important biological species ranging from simple ions to complex biomolecules. The ability to miniaturize electrochemical sensors, coupled with their affordability and simple equipment requirements for signal readout, permits the use of these sensors at the point-of-care where analysis using
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Size-related variability of oxygen consumption rates in individual human hepatic cells Lab Chip (IF 6.1) Pub Date : 2024-07-26 Ermes Botte, Yuan Cui, Chiara Magliaro, Maria Tenje, Klaus Koren, Andrea Rinaldo, Roman Stocker, Lars Behrendt, Arti Ahluwalia
Accurate descriptions of the variability in single-cell oxygen consumption and its size-dependency are key to establishing more robust tissue models. By combining microfabricated devices with multiparameter identification algorithms, we demonstrate that single human hepatocytes exhibit an oxygen level-dependent consumption rate and that their maximal oxygen consumption rate is significantly lower than
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Two-dimensional high performance liquid chromatography purification of underivatized urinary prednisone and prednisolone for compound-specific stable carbon isotope analysis Analyst (IF 3.6) Pub Date : 2024-07-26 Quan Zhong Li, Bing Liu
The gas chromatography-combustion isotope ratio mass spectrometry (GC/C/IRMS) confirmation procedure for prednisone and prednisolone is still a great challenge for the doping control laboratory due to the many structurally similar steroids present in urinary matrices. This study aims to establish an innovative online two-dimensional high performance liquid chromatography (2D-HPLC) purification method
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I-motif sensor for the fluorometric detection of Monkeypox Analyst (IF 3.6) Pub Date : 2024-07-26 Tasnima Alam Asa, Young Jun Seo
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Analytical and Functional Characterization of Plasmid DNA Topological Forms and Multimers Anal. Chem. (IF 6.7) Pub Date : 2024-07-25 Daniel Ngoc Nguyen, Peggy Ko, Brian Roper, Gary Console, Yuan Gao, David Shaw, Christopher Yu, Peter Yehl, Kelly Zhang, Alexandre Goyon
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Rapid Screening of Biomarkers in KYSE-150 Cells Exposed to Polycyclic Aromatic Hydrocarbons via Inkjet Printing Single-Cell Mass Spectrometry Anal. Chem. (IF 6.7) Pub Date : 2024-07-25 Ai Ren, Fengming Chen, Chenjie Ren, Minli Yang, Chang Wang, Xuesong Feng, Feng Zhang
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Direct Enantiomer Differentiation of Drugs and Drug-Like Compounds via Noncovalent Copper–Amino Acid Complexation and Ion Mobility-Mass Spectrometry Anal. Chem. (IF 6.7) Pub Date : 2024-07-25 Benjamin K. Blakley, Emanuel Zlibut, Rashi M. Gupta, Jody C. May, John A. McLean
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In Situ High-Precision Measurement of Deep-Sea Dissolved Methane by Quartz-Enhanced Photoacoustic and Light-Induced Thermoelastic Spectroscopy Anal. Chem. (IF 6.7) Pub Date : 2024-07-24 Hao Liu, Xiang Chen, Mai Hu, Haoran Wang, Lu Yao, Zhenyu Xu, Guosheng Ma, Qiang Wang, Ruifeng Kan
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Levodopa: From Biological Significance to Continuous Monitoring ACS Sens. (IF 8.2) Pub Date : 2024-07-24 David Probst, Kartheek Batchu, John Robert Younce, Koji Sode
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Research Progress of Flexible Piezoresistive Sensors Based on Polymer Porous Materials ACS Sens. (IF 8.2) Pub Date : 2024-07-24 Song Han, Sheng Li, Xin Fu, Shihui Han, Huanyu Chen, Liu Zhang, Jun Wang, Gaohui Sun
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Extremely Sensitive Genetically Encoded Temperature Indicator Enabling Measurement at the Organelle Level ACS Sens. (IF 8.2) Pub Date : 2024-07-23 Shun-ichi Fukushima, Tetsuichi Wazawa, Kazunori Sugiura, Takeharu Nagai
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Phase-Engineered MoSe2/CeO2 Composites for Room-Temperature Gas Sensing with a Drastic Discrimination of NH3 and TEA Gases ACS Sens. (IF 8.2) Pub Date : 2024-07-23 Sukhwinder Singh, Ka Yoon Shin, Sungjoon Moon, Sang Sub Kim, Hyoun Woo Kim
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Label-Free Detection of DNA Base Mutation and Hybridized DNA by an Electrostatically Modified 3D Plasmonic Array ACS Sens. (IF 8.2) Pub Date : 2024-07-23 Xiaolu Xing, Yuhao Zhao, Haiyan Zhao, Ying Zhang, Ziqiu Chen, Bowen Liu, Bin Ren
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Construction of Fully Integrated and Energy Self-Sufficient NO2 Gas Sensors Utilizing Zinc-Air Batteries ACS Sens. (IF 8.2) Pub Date : 2024-07-22 Feifei Li, Xiao Wang, Xixi Li, Yao Fu, Zhaokun Sun, Keyang Zhao, Cunguang Zhu, Xijin Xu
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Surface-Enhanced Raman Scattering Monitoring of Tryptophan Dynamics in 3D Pancreatic Tumor Models ACS Sens. (IF 8.2) Pub Date : 2024-07-22 Pablo S. Valera, Margarida Henriques-Pereira, Marita Wagner, Vítor M. Gaspar, João F. Mano, Luis M. Liz-Marzán
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A multiplexed, allele-specific recombinase polymerase amplification assay with lateral flow readout for sickle cell disease detection Lab Chip (IF 6.1) Pub Date : 2024-07-25 Megan M. Chang, Mary E. Natoli, Alexis F. Wilkinson, Venée N. Tubman, Gladstone E. Airewele, Rebecca R. Richards-Kortum
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Nanofluidic resistive pulse sensing for characterization of extracellular vesicles Lab Chip (IF 6.1) Pub Date : 2024-07-25 Madalena R. C. Calado, Teresa C. Lage, Daniel A. M. André, Carlos Calaza, Carlos Marques, Carolina Herrero, João Piteira, Lars Montelius, Dmitri Y. Petrovykh, Lorena Diéguez, Alar Ainla
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Roll-to-Roll Manufacturing of Large Surface Area PDMS Devices, and Application to a Microfluidic Artificial Lung Lab Chip (IF 6.1) Pub Date : 2024-07-24 Andrew Zhang, Kartik Tharwani, Jennifer Wang, Gabriele Karl Seilo, Michael Atie, Joseph Allen Potkay
The ability to cost-effectively produce large surface area microfluidic devices would bring many small-scale technologies such as microfluidic artificial lungs (μALs) from the realm of research to clinical and commercial applications. However, efforts to scale up these devices, such as by stacking multiple flat μALs have been labor intensive and resulted in bulk devices. Here, we report an automated
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Efficient discovery of antibody binding pairs using a photobleaching strategy for bead encoding Lab Chip (IF 6.1) Pub Date : 2024-07-24 Shira Roth, Thomas Ferrante, David R Walt
Dye-encoded bead-based assays are widely used for diagnostics. Multiple bead populations are required for multiplexing and can be produced using different dye colors, labeling levels, or combinations of dye ratios. Ready-to-use multiplex bead populations restrict users to specific targets, are costly, or require specialized instrumentation. In-house methods produce few bead plexes or require many fine-tuning
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A novel microfluidic tool for the evaluation of local drug delivery systems in simulated in vivo conditions Lab Chip (IF 6.1) Pub Date : 2024-07-24 William A. Oates, Antonios D. Anastasiou
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A pumpless liver-on-a-chip for drug hepatotoxicity analysis Analyst (IF 3.6) Pub Date : 2024-07-25 Dian Jiao, Lan Xie, Wanli Xing
This study presents the development and validation of an innovative microfluidic liver-on-a-chip device utilizing gravity-driven perfusion for the evaluation of drug hepatotoxicity. This research involved the construction of a hydrogel-based coculture chip that integrates liver parenchymal and stellate cells within a tri-channel configuration. The assembly and operation of the liver-on-a-chip and its
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Efficient separation of large particles and giant cancer cells using an isosceles trapezoidal spiral microchannel Analyst (IF 3.6) Pub Date : 2024-07-22 Chanyong Park, Wanyoung Lim, Ryungeun Song, Jeonghun Han, Daeun You, Sangmin Kim, Jeong Eon Lee, Danny van Noort, Carl-Fredrik Mandenius, Jinkee Lee, Kyung-A. Hyun, Hyo-Il Jung, Sungsu Park
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Surface Sialic Acid Detection of Small Extracellular Vesicles at the Single-Particle Level by Nano-Flow Cytometry Anal. Chem. (IF 6.7) Pub Date : 2024-07-24 Niangui Cai, Xiaozhen Zhan, Yan Chen, Junwei Xue, Chen Chen, Yurou Li, Ye Tian, Xiaomei Yan
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Ratiometric Readout of Bacterial Infections via a Lyophilized CRISPR–Cas12a Sensor with Color-Changeable Bioluminescence Anal. Chem. (IF 6.7) Pub Date : 2024-07-24 Yu Wang, Xiaoning Xu, Jinqi Que, Xinyu Wang, Wei Ni, Yunhua Wu, Liu Yang, Yong Li
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“One-Pot” Readout Cyano-Programmable SERS-Encoded Platform Enables Ultrasensitive and Interference-Free Detection of Multitarget Bioamines Anal. Chem. (IF 6.7) Pub Date : 2024-07-24 Chen Chen, Xinyue Wang, Ximo Wang, Geoffrey I. N. Waterhouse, Mingdi Jiang, Xuguang Qiao, Zhixiang Xu
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Evaluation of a Liquid Chromatography–Tandem Mass Spectrometry Method for the Analysis of Glucosylceramide and Galactosylceramide Isoforms in Cerebrospinal Fluid of Parkinson’s Disease Patients Anal. Chem. (IF 6.7) Pub Date : 2024-07-24 Laura Castillo-Ribelles, Jose Antonio Arranz-Amo, Jorge Hernández-Vara, Daniela Samaniego-Toro, Silvia Enriquez-Calzada, Sara Lucas-Del Pozo, Maria Camprodon-Gomez, Ariadna Laguna, Mercedes Arrúe Gonzalo, Roser Ferrer, Marta Martinez-Vicente, Clara Carnicer-Caceres
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Effects of Relative Humidity and Phase on the Molecular Detection of Nascent Sea Spray Aerosol Using Extractive Electrospray Ionization Anal. Chem. (IF 6.7) Pub Date : 2024-07-24 Samantha M. Kruse, Paul R. Tumminello, Alexia N. Moore, Christopher Lee, Kimberly A. Prather, Jonathan H. Slade
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Sensitive Multiplexed MicroRNA Spatial Profiling and Data Classification Framework Applied to Murine Breast Tumors Anal. Chem. (IF 6.7) Pub Date : 2024-07-23 Omar N. Mohd, Yujing J. Heng, Lin Wang, Abhishek Thavamani, Erica S. Massicott, Gerburg M. Wulf, Frank J. Slack, Patrick S. Doyle
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Target-Triggered Aggregation of Modified E. coli for Diagnosis of Ovarian Cancer Anal. Chem. (IF 6.7) Pub Date : 2024-07-23 Zheying Mu, Yujing Zeng, Siyu Liu, Weikang Ge, Shiao Yang, Chenbo Ji, Xuemei Jia, Genxi Li
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Construction of Cell Membrane Chromatography Screening Materials Based on Avi-Tag Fused G Protein-Coupled Receptors Anal. Chem. (IF 6.7) Pub Date : 2024-07-23 Yanni Lv, Yamin Wang, Na Guo, Haoyun Bai, Yuhan Jiang, Yihan Huang, Hongfen Du, Shengli Han, Langchong He
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Surface functionalization of chalcogenide IR photonic sensor by means of polymer membrane devoted to water pollution Analyst (IF 3.6) Pub Date : 2024-07-24 Martin Vrazel, Kadar-Ismail Raissa, Rémi Courson, Hammouti Abdelali, Marek Bouska, Larrode Amelie, Baillieul Marion, Giraud William, Stephane Le Floch, Loic Bodiou, Joël Charrier, Boukerma Kada, MICHEL Karine, Petr Nemec, Virginie Nazabal
Rapid, simultaneous detection of organic chemical pollutants in water is an important issue in regards of preserving human health. In this study, the possibility of developing in situ reusable optical sensor capable of selective measurements, utilizing chalcogenide transducer supplemented by hydrophobic polymer membrane and build on detection by evanescent wave in mid-infrared spectrum is evaluated
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Antenna effect enhanced ECL immunoassay using microfloral europium porphyrin coordination polymers based on Eu3+ and TCPP for the detection of chloramphenicol in foods Analyst (IF 3.6) Pub Date : 2024-07-24 Xinyue Qian, Cheng Tan, Jing Zhang, Kang Wu, Anping Deng, Jianguo Li
The “antenna effect” is one of the most important energy transfer modes in lanthanide light-emitting polymers. In this study, novel luminescent nanostructured coordination polymers (Eu-PCP) were synthesized in one step using Eu3+ as the central metal ion and 5,10,15,20-tetrakis (4-carboxyphenyl) porphyrin (TCPP) as the organic ligand. Unique “antenna effect” observed between Eu3+ and TCPP leads to
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LA-ICPMS for In Situ U–Th Dating of Holocene Stalagmites Anal. Chem. (IF 6.7) Pub Date : 2024-07-23 Chung-Che Wu, Chuan-Chou Shen, Detlef Günther, Bodo Hattendorf
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Reversible Hydrophobic Deep Eutectic Solvent-Based Uranyl-Sensing Optode Film in Aqueous Streams: Color Transformation and Reusability Anal. Chem. (IF 6.7) Pub Date : 2024-07-23 Komal C. Shrivastava, K. S. Ajish Kumar, Arijit Sengupta, Sheikh Musharaf Ali, Jayshree Ramkumar
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Light-Driven Electrochemical Biosensing with DNA Origami-Assisted Hybrid Nanoantenna for Fumonisin B1 Monitoring Anal. Chem. (IF 6.7) Pub Date : 2024-07-23 Na Dong, Shuda Liu, Shuyun Meng, Zuo Chen, Yuye Li, Dong Liu, Tianyan You
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Endogenous Glutathione-Activated Nucleic Acid Molecular Circuitry for Cell-Specific MicroRNA Imaging Anal. Chem. (IF 6.7) Pub Date : 2024-07-23 Hui Wang, Yingying Chen, Yuqian Jiang, Yifei Wang, Ruomeng Li, Jinhua Shang, Fuan Wang
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1,1,2,2-Tetra(4-carboxylphenyl)ethylene-Based Metal–Organic Gel as Aggregation-Induced Electrochemiluminescence Emitter for the Detection of Aflatoxin B1 Based on Nanosurface Energy Transfer Anal. Chem. (IF 6.7) Pub Date : 2024-07-23 Yue Jia, Xiaoyue Zhang, Xuan Kuang, Dawei Fan, Xu Sun, Xiang Ren, Hongmin Ma, Dan Wu, Qin Wei
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High-Throughput Raman Spectroscopy by Horizontally Shifted Collection Fibers Anal. Chem. (IF 6.7) Pub Date : 2024-07-22 Koseki J. Kobayashi-Kirschvink, Alex Matlock, Peter T. C. So, Jeon Woong Kang
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Development of a Colorimetric and SERS Dual-Signal Platform via dCas9-Mediated Chain Assembly of Bifunctional Au@Pt Nanozymes for Ultrasensitive and Robust Salmonella Assay Anal. Chem. (IF 6.7) Pub Date : 2024-07-22 Han Jiang, Wei Chang, Xiaofan Zhu, Gang Liu, Kaiyong Liu, Wei Chen, Hua Wang, Panzhu Qin
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3D-Printed Microelectrodes for Biological Measurement Anal. Chem. (IF 6.7) Pub Date : 2024-07-22 Zehao Xue, Kanisha Patel, Paankhuri Bhatia, Chloe L. Miller, Ricoveer Singh Shergill, Bhavik Anil Patel
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3D cellular self-assembly on optical discs-imprinted nanopatterns Lab Chip (IF 6.1) Pub Date : 2024-07-23 Jeeyeon Lee, Chwee Teck Lim
Three-dimensional (3D) cellular assemblies such as cancer spheroids, or organoids, are gaining recognition for their physiological relevance, and versatility in various biological applications. Nanopatterns mimicking the extracellular matrix provide crucial topological cues guiding cellular behaviors, potentially facilitating 3D cell self-assembly. However, the widespread adoption of nanopatterns in
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A candidate reference measurement procedure for the quantification of α-synuclein in cerebrospinal fluid using an SI traceable primary calibrator and multiple reaction monitoring Analyst (IF 3.6) Pub Date : 2024-07-23 Leran Zhang, Eva Illes-Toth, Adam Cryar, Giles Drinkwater, Lucia Di Vagno, Marie-Laure Pons, Julia Mateyka, Bryan McCullough, Eli Achtar, Cailean Clarkson, Laura Göschel, Peter Körtvélyessy, Chris Mussell, Christopher J. Hopley, Agnes Flöel, Christophe Hirtz, Sylvain Lehmann, Milena Quaglia
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Low-cost heat assisted ambient ionization source for mass spectrometry in food and pharmaceutical screening Analyst (IF 3.6) Pub Date : 2024-07-19 Odhisea Gazeli, Efstathios A. Elia, Nikolaos Argirusis, Constantinos Lazarou, Charalambos Anastassiou, Joachim Franzke, Juan F. Garcia-Reyes, George E. Georghiou, Agapios Agapiou
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Novel mycophenolic acid precursor-based fluorescent probe for intracellular H2O2 detection in living cells and Daphnia magna and Zebrafish model systems Analyst (IF 3.6) Pub Date : 2024-07-18 Jongkeol An, Sujeong Park, Neha Jain, Youngsam Kim, Satish Balasaheb Nimse, David G. Churchill
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Supported gel slab scaffolds as a three-dimensional cell-based assay platform Analyst (IF 3.6) Pub Date : 2024-07-22 Zachary Ryan Sitte, Elizabeth E. Karlsson, Tyler S. Larson, Haolin Li, Haibo Zhou, Matthew Ryen Lockett
Cell-based assays are heavily relied on in the drug discovery pipeline, quickly pairing down large compound libraries to a manageable number of drug candidates for further characterization and evaluation. Monolayer cultures in which cells are deposited onto the bottom of well plates are the workhorse of many of these screens despite continued evidence of their inability to predict in vivo responses
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Simultaneous determination of the 18 steroids in hypotha-lamic pituitary gonadal axis based on UPLC–MS/MS with multimode ionization Analyst (IF 3.6) Pub Date : 2024-07-22 Yinan Zhang, Guanghui Wang, Yongjian Shao, Pei Zheng, Chunyan Guo, Zhiqun Liu, Lilai Shen, Zhiquan Liu, Jiafeng Ding, Hangjun Zhang
To objectively quantify changes in steroid hormones in organisms caused by adverse environmental loads, we developed a simple and sensitive UPLC–MS/MS (ultra-performance liquid chromatography triple quadrupole mass spectrometry) method for the simultaneous determination of 18 steroid hormones on the HPG axis. This analytical method based on liquid extraction and a multimode electrospray and at-mospheric
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FREE: Enhanced Feature Representation for Isotopic Envelope Evaluation in Top-Down Mass Spectra Deconvolution Anal. Chem. (IF 6.7) Pub Date : 2024-07-22 Jiancheng Zhong, Xingran Song, Shaokai Wang
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A Label-Free Sensor Array Based on Carbon Quantum Dots for Detection of α-Synuclein Oligomers in Saliva Anal. Chem. (IF 6.7) Pub Date : 2024-07-22 Zeinab Saedi, Mohammad Javad Masroor, Atieh Jahangiri-Manesh, Aram Rezaei, Karolina Siskova, Maryam Nikkhah
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Investigating Lipid Transporter Protein and Lipid Interactions Using Variable Temperature Electrospray Ionization, Ultraviolet Photodissociation Mass Spectrometry, and Collision Cross Section Analysis Anal. Chem. (IF 6.7) Pub Date : 2024-07-22 Virginia K. James, Bradley J. Voss, Amanda Helms, M. Stephen Trent, Jennifer S. Brodbelt
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Hyperpolarized Nano-NMR Platform for Quantification of Mass Limited Samples Anal. Chem. (IF 6.7) Pub Date : 2024-07-22 Roberta Pigliapochi, Thasin Peyear, Thomas Ruan, Kayvan R. Keshari
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DMF-DM-seq: Digital-Microfluidics Enabled Dual-Modality Sequencing of Single-Cell mRNA and microRNA with High Integration, Sensitivity, and Automation Anal. Chem. (IF 6.7) Pub Date : 2024-07-22 Yingwen Chen, Xuanqun Wang, Xing Na, Yingkun Zhang, Linfeng Cai, Jia Song, Chaoyong Yang
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Probing Short-Range Interactions in Isostructural Hydrate and Perhydrate of Dabrafenib by Magic-Angle Spinning Solid-State NMR Anal. Chem. (IF 6.7) Pub Date : 2024-07-21 Sunil K. Rai, Lokeswara Rao Potnuru, Nghia Tuan Duong, Toshio Yamazaki, Ashwini K. Nangia, Yusuke Nishiyama, Vipin Agarwal
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FAIMS Shotgun Lipidomics for Enhanced Class- and Charge-State Separation Complemented by Automated Ganglioside Annotation Anal. Chem. (IF 6.7) Pub Date : 2024-07-19 Katharina Hohenwallner, Leonida M. Lamp, Liuyu Peng, Madison Nuske, Jürgen Hartler, Gavin E. Reid, Evelyn Rampler
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Pd Nanoclusters-Sensitized MIL-125/TiO2 Nanochannel Arrays for Sensitive and Humidity-Resistant Formaldehyde Detection at Room Temperature ACS Sens. (IF 8.2) Pub Date : 2024-07-22 Jiahui Zhao, Haiquan Wang, Zhen-kun He, Wenwen Zhang, Yang Du, Xinze Li, Shujia Wang, Junjian Zhao, Yan-Yan Song, Zhida Gao
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A programmable and automated optical electrowetting-on-dielectric (oEWOD) driven platform for massively parallel and sequential processing of single cell assay operations Lab Chip (IF 6.1) Pub Date : 2024-07-22 Lawrence G. Welch, Jasper Estranero, Panagiotis Tourlomousis, Robert C. R. Wootton, Valentin Radu, Carlos González-Fernández, Tim J. Puchtler, Claire M. Murzeau, Nele M. G. Dieckmann, Aya Shibahara, Brooke W. Longbottom, Clare E. Bryant, Emma L. Talbot
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Optimized microfluidic formulation and organic excipients for improved lipid nanoparticle mediated genome editing Lab Chip (IF 6.1) Pub Date : 2024-07-22 Rohan Palanki, Emily L. Han, Amanda M. Murray, Rohin Maganti, Sophia Tang, Kelsey L. Swingle, Dongyoon Kim, Hannah Yamagata, Hannah C. Safford, Kaitlin Mrksich, William H. Peranteau, Michael J. Mitchell
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A Lateral Flow Biosensor Based on Isothermal Amplification for Visual Identification of Species and Sex from Bloodstain Anal. Chem. (IF 6.7) Pub Date : 2024-07-20 Huyun Zhou, Wenjing Hu, Jiaxing Zhang, Ting Ma, Xiaonan Liu, Jiangwei Yan
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Online Charge-Generation Derivatization by Electrochemical Radical Cations of Thianthrene: Mass Spectrometry Imaging of Estrogens in Biological Tissues Anal. Chem. (IF 6.7) Pub Date : 2024-07-20 Yingchao Liu, Jiahui Bai, Xiaoxia Dong, Yuqi Cao, Mingmai Bao, Yingjie Lu, Hui Zeng, Lixing Zhan, Yinlong Guo
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Sulfonic Acid-Functionalized Tetraphenylethylene-Amplified Electrochemiluminescence by Regulating π–π Interaction Anal. Chem. (IF 6.7) Pub Date : 2024-07-20 Yizhuo Fu, Jingke Pan, Yuhao Liu, Chao Lu