The aim of this work is to study a bifunctional endoglucanase/carboxylesterase in Sphingobacterium soilsilvae Em02 and express it in soluble form in engineered Escherichia coli. The molecular weight of the recombinant protein of the bifunctional enzyme was 41 KDa. This research also determined the enzymatic activities of the bifunctional enzymes using microcrystalline cellulose and p-nitrophenyl butyrate

Recycling waste gases from industry is vital for the transition toward a circular economy. The model microbe Clostridium ljungdahlii reduces carbon from syngas and primarily produces acetate and ethanol. Here, a gas fermentation experiment is presented in chemostats with C. ljungdahlii and pure carbon monoxide (CO) as feedstock while entirely omitting yeast extract. A maximum ethanol production rate

Heavy metals in livestock manure pose a threat to the environment after biogas fertilizer being utilized, while its bioavailability is reduced substantially by passivator during the anaerobic digestion. In this study, an optimal composite passivator of humic acid, fly ash and biochar with proportion of 7.5%:7.5%:7.5% and 5.0%:7.5%:7.5% is obtained and the passivation mechanism on Cu and Zn during anaerobic

Bioenergy production is the most sought-after topics at the crunch of energy demand, climate change and waste generation. In view of this, lignocellulosic biomass (LCB) rich in complex organic content has the potential to produce bioenergy in several forms following the pretreatment. Hydrothermal pretreatment that employs high temperatures and pressures is gaining momentum for organics recovery from

The present work aims to assess the treatment of unprocessed urban wastewater using the microalga Scenedesmus almeriensis. Two 12 m3 raceway reactors, one supplemented by wastewater and the second by chemical fertilizer, operating outdoors in a semi-continuous mode, were used for eight months. Results suggested that S. almeriensis can be produced in wastewater without affecting the photosynthetic apparatus

In this study, exogenous Megasphaera elsdenii inoculum and acetate supplementation were introduced at the acidogenic phase to regulate the acidogenic fermentation pathway and assess their effects on food waste (FW) carbon conversion in two-phase anaerobic digestion (AD) system. These two additives significantly accelerated organic removal efficiency and subsequently increased FW hydrolysis and acidogenesis

Microwave-assisted heating is an effective heating method for thermochemical conversion of biomass. In this study, experimental syngas production from microwave-assisted air gasification of biomass in a fluidized bed reactor at different gasification temperatures, equivalence ratios and silicon carbide loads, was studied and reported. The results showed that the highest syngas yield (78.2 wt.%), higher

Co-composting of forest residues (FR) with cattle manure (CM) results in a low nitrogen (N) conversion efficiency, a low organic matter (OM) degradation rate, and a low quality compost product. This study evaluated the effects of addition of bone charcoal (BC), pumice (PM), or straw biochar (SB) at a ratio of 10% (w/w) on the co-composting of FR with CM. The highest quality compost was obtained with

Lignocellulose is resistant to degradation and requires pretreatment before hydrolytic enzymes can release fermentable sugars. Sulfuric acid has been widely used for biomass pretreatment, but high amount of degradation products usually occurred when using this method. To enhance accessibility to cellulose, we studied the performances of several dilute organic acid pretreatments of sugarcane bagasse

Substrate toxicity would limit the upgrading of waste biomass to medium-chain fatty acids (MCFAs). In this work, two fermentation modes of electro-fermentation (EF) and traditional fermentation (TF) with different concentration of liquor fermentation waste (20%, 40%, 60%) were used for MCFAs production as well as mechanism investigation. The highest caproate (4.04 g/L) and butyrate (13.96 g/L) concentrations

In this study, an innovative approach was developed to enhance the hydrolysis through phase-separated pretreatment by removing exopolymeric substance via mild sonication followed by thermo-Fenton disintegration. The exopolymeric substance fragmentation was enhanced at the sonic specific energy input of 2.58 kJ/kg total solids. After exopolymeric substance removal, the disintegration of biomass by thermo-Fenton

Polybrominated diphenyl ethers (PBDEs), derived from electronics, furniture, etc., are detected with high level in excess sludge (ES). In this work, the influence of PBDEs on ES dark fermentation (ESDF) hydrogen production and the related key mechanisms were explored. The result shows PBDEs exposure reduced hydrogen production, and hydrogen accumulation decreased from 17.6 mL/g in blank to 12.3 mL/g

Recycling cellulase can reduce the cost of lignocellulosic enzymatic hydrolysis. Here, a lignin-grafted sulfobetaine (LSB) was first synthesized by grafting sulfobetaine (SB) on enzymatic hydrolysis lignin (EHL). LSB had a sensitive response of pH and temperature. LSB dissolved under the conditions of lignocellulosic enzymatic hydrolysis (pH 5.0, 50 °C). After hydrolysis, LSB co-precipitated with cellulase

Lignocellulose represents the most abundant carbon-capturing substance that is convertible for biofuels and bioproduction. Although biomass pretreatments have been broadly applied to reduce lignocellulose recalcitrance for enhanced enzymatic saccharification, they mostly require strong conditions with potential secondary waste release. By classifying all major types of pretreatments that have been

Biorefineries integrate processes for the sustainable conversion of biomass into chemicals, materials, and bioenergy so that resources are optimized and effluents are minimized. Despite the vast potential of lignocellulosic biorefineries, their success depends heavily on effective, economically viable, and sustainable biomass fractionation. Although efficient, organosolv pretreatment still faces challenges

The production of high-value products from lignocellulosic biomass is carried out through the selective scission of crosslinked C–C/C–O bonds. Several techniques are applied nowadays to optimize biomass conversion into desired products with high yields. Photocatalytic technology has been proven to be a valuable tool for valorizing biomass at mild conditions. The photoproduced reactive oxygen species

Lignocellulose biomass during pretreament release various copmounds, among them important one is reducing sugars, which can be utilized for the production of biofuels and some other products. Thereby, innovative greener pretreatment techniques for lignocellulosic materials have been considered to open a new door in the aspects of digestibility of the rigid carbohydrate–lignin matrix to reduce the particle

To resolve environmental problems associated with rice straw and silage effluent disposal, silage effluent pretreating rice straw for the anaerobic production of volatile fatty acids (VFAs) was investigated. To prevent the lactic acid bacteria in silage effluent from inhibiting anaerobic fermentation, four phenyllactic acid (PLA) levels were set (0, 0.1, 0.3, 0.5 mg/kg). The total VFA yields of treatments

Abstract not available

Bio–wastes and their utilization has been increasing enormously, due to its generation and management practices towards making the clearner environment. Bio–waste disposal that follow the emerging global human population has commended the hunt to certain methods sustainably for the bio–waste management to overwhelmed the ecological issues, prompted by means of the collection of such waste materials