The use of agricultural biomass-based fertilizers, and the release of feces into the environment leads to last-lasting pollution of antibiotic resistance genes that cannot be removed from waters via traditional methods, resulting in significant health threats. To solve this issue, an antibiotic resistance gene removal method was proposed and tested that used sequence-specific DNA-binding designer zinc

The algal-bacterial symbiosis system (ABSS) is considered as a sustainable wastewater treatment process. This review provides a comprehensive overview of the mechanisms of ABSS for the removal of common pollutant, heavy metals, and especially for emerging pollutants. For the macroscopical level, this review not only describes in detail the reactor types, influencing factors, and the development of

The carbon and energy efficiency of a biomanufacturing process is of crucial importance in determining its economic viability. Formate dehydrogenase has been demonstrated to be beneficial in regenerating NADH from formate produced during sugar metabolism, thereby creating energy-efficient systems. Nevertheless, introducing enzyme(s) for butyryl butyrate (BB) biosynthesis based on this system, only

The aim of this study was to investigate the effects of various concentrations of antioxidants, including butyl hydroxy anisd (BHA), butylated hydroxytoluene (BHT), fulvic acid (FA), melatonin (MT), glycine betaine (GB) and putrescine (Put), on growth and lipid synthesis of microalgae under low-temperature (15 ℃). Changes in biochemical indicators, reactive oxygen species (ROS) level, glutathione (GSH)

Cordycepin, a nucleoside analog, is widely used in medicine and health products. However, the production of cordycepin from faces the challenges of low productivity and high rate of greenhouse gas emissions. In this study, by optimizing the cordycepin biosynthesis pathway through promoter combination, Kozak sequence, and enzyme fusion, enhancing the methanol assimilation capacity in peroxisomes, adjusting

Gamma-Aminobutyric acid (GABA) is a derivative of -glutamate, also a precursor for the synthesis of 2-pyrrolidone, which is a monomer of nylon-4. This study achieved a one-step biosynthesis of GABA and 2-pyrrolidone by overexpressing key genes involved in GABA and 2-pyrrolidone synthesis and deleting GABA degradation genes combined with reducing the degradation of 2-pyrrolidone precursor. The resulting

This study investigates the potential of TCPF to enhance phosphate recovery and nutrient bioavailability from sewage sludge ash (SSA) and fish meal (FM) through co-fermentation. The fungal treatment was found to significantly increase phosphate recovery, achieving up to 16 % efficiency, especially at a 10 g/L waste concentration. The key mechanism behind this enhancement is the production of low molecular

This study investigated the reduction of methane (CH) and nitrous oxide (NO) emissions from stormwater bioretention cells through microbial electrolytic cell (MEC), showing the largest reduction of 32.21 % (CH) at 9.2 μA/m of current density and 56.16 % (NO) at 3.5 μA/m of current density, compared with the corresponding in the control (0 μA/m of current density). Kinetic of CH and NO emissions could

Microbial degradation plays a crucial role in removing sulfonamides from soil, enhancing sulfamethoxazole (SMX) remediation. To further augment SMX removal efficiency and mitigate the transmission risk associated with antibiotic resistance genes (ARGs), this study proposes a novel approach that integrates micro-animals, microorganisms, and microbial fuel cell (MFC) technology. The results showed that

Two parallel pilot-scale reactors were operated to investigate pollutant removal performance and metabolic pathways in elemental sulfur-driven autotrophic denitrification (SDAD) process under low temperature and after addition of external electron donors. The results showed that low temperature slightly inhibited SDAD (average total nitrogen removal of ∼4.7 mg L) while supplement of sodium thiosulfate

This study assessed the characteristics and toxicity of aqueous pyrolytic liquid (APL) derived from digested sewage sludge on anaerobic digestion (AD) and determined its rate-limiting step. Digested sewage sludge was pyrolyzed at multiple temperatures (350–650 °C) and moisture levels (0–40.4 %), resulting in APLs with varying AD toxicities. APL 350 °C-0 % showed the least toxicity, whereas APL 650 °C-40

Utilization of organic community wastes towards deriving sustainable renewable energy and adequate disposal of the residual has been an important topic of investigation. Anaerobic digestion and co-digestion of rice-derived food waste and animal manure for sustainable biogas generation is crucial from the view-point of community consumption. This paper presents an extensive review of the important and

In this study, the effect of modulating fulvic acid (FA) concentrations (0, 25 and 50 mg/L) on nitrogen removal in a bioelectrochemical hydrogen autotrophic denitrification system (BHDS) was investigated. Results showed that FA increased the nitrate (NO-N) removal rate of the BHDSs from 37.8 to 46.2 and 45.2 mg N/(L·d) with a current intensity of 40 mA. The metagenomic analysis revealed that R2 (25 mg/L)

An innovative binary biol-based deep eutectic solvent (DES), specifically ethylamine hydrochloride-ethylene glycol (EaCl-EG), was developed for efficient pretreatment of eucalyptus biomass. This DES exhibited superior performance in achieving high delignification (85.0%) and xylan removal (80.0%), while preserving a significant amount of cellulose (94.5%) compared to choline chloride-based DES. Notably

Anaerobic digestion (AD) is an environmentally friendly technology that simultaneously stabilizes biowaste and produces biogas. Conventional AD faces challenges such as inadequate substrate degradation and low methane purity. Pressure-centric regulation serves as an AD optimization strategy that can enhance the digestion efficiency and generate higher-energy-value biogas. However, limited reviews have

In this study, ADM1-based kinetics were combined with a thermal model that accounts for various heat transfers inside and through the reactor’s boundaries. Computing the energy of bioreactions based on kinetic rates prevented an overestimation of approximately 20% in the heat demand of the heat exchanger, compared to calculations using feedstock degradation heat. This also improved the representation

The effect of different levels of temperature on resistance genes is not clear in mesophilic static composting (<50 °C). This study conducted livestock manure composting with different temperature gradients from 20 to 50 °C, it was found that the reduction rates of risk rank-I antibiotic resistance genes (from 3 % to 66 %), metal resistance genes (from −50 % to 76 %) and bacterial pathogens (from 72 %

has emerged as a prominent candidate for producing single-cell oil from cost-effective feedstocks. In this study, the capability of to produce punicic acid (PuA), a representative plant unusual fatty acid, was investigated. The introduction of acyl lipid desaturase and conjugase (PgFADX) allowed to accumulate 3.7 % of total fatty acids as PuA. Delta-12 acyl lipid desaturase (PgFAD2) and diacylglycerol

Baffled flow constructed wetland-microbial fuel cell (BFCW-MFC) coupling systems were constructed with baffles embedded in cathode chamber. The performance of BFCW-MFCs operated at different hydraulic retention times (HRTs) was evaluated. At the representative HRT of 48 h, embedding 1 or 2 baffles (i.e., BFCW-MFC1 and BFCW-MFC2) produced 32.9 % (29.5 mW/m) and 53.2 % (34.0 mW/m) more power density

Bacterioruberin is widely used in medicine, food, and cosmetics owing to its prominent characteristics of antioxidants and bioactivities. Bioconversion of methane into bacterioruberin is a promising way to address biomanufacturing substrate costs and greenhouse gas emissions but has not been achieved yet. Herein, this study aimed to upcycle methane to bacterioruberin by microbial consortia. The microbial

Phenol, quinoline, and pyridine, commonly found in industrial wastewater, disrupt the nitrification process, leading to nitrite accumulation. This study explores the potential mechanisms through which these biotoxic organic compounds affect nitrite accumulation, using metagenomic and molecular docking analyses. Despite increasing concentrations of these compounds from 40 to 160 mg/L, ammonia nitrogen

Simultaneous nitrification–denitrification (SND) is a promising nitrogen removal process. However, total nitrogen (TN) removal is limited due to unsatisfactory denitrification. This study demonstrated that short-time (1 h) pre-anoxic electro-stimulation significantly enhanced SND efficiency in the aerobic phase by promoting the proliferation of mixotrophic and heterotrophic denitrifiers. SND and TN

Landfill leachate contains ammonium and micropollutants. Ammonium can be biologically removed but bio-recalcitrant micropollutants removal requires post-treatment like ozonation. This study developed an expanded clay aggregates packed biofilm column (EBC) and demonstrated its feasibility of coupling biodegradation and ozonation (CBAO) to simultaneously remove nitrogen and bio-recalcitrant micropollutants

Recently, the growing health awareness of society on the utilization of fabrics has led to an increasing demand for natural-based antibacterial textiles. Lignin, a generous polyphenol compound in nature, is capable of preventing bacterial growth; in particular, it dwells bacteria closely together on human skin, such as , , , and . However, the antibacterial properties of lignin are limited by factors

Anaerobic electrochemical digestion (AED) is an effective system for recovering biogas from organic wastes. However, the effects of different anode potentials on anaerobic activated sludge remain unclear. This study confirmed that biofilms exhibited the best electroactivity at −0.2 V (vs. Ag/AgCl) compared to −0.4 V and 0 V. Gas was further regulated, with the highest hydrogen content (47 ± 7 %) observed

Temperature is the critical factor affecting the efficiency and cost of anaerobic digestion (AD). The current work develops a shift-temperature AD (STAD) between 35 °C and 55 °C, intending to optimise microbial community and promote substrate conversion. The experimental results showed that severe inhibition of biogas production occurred when the temperature was firstly increased stepwise from 35 °C

Sewage sludge derived fertilizer is a promising solution for phosphorus (P) recovery from biowaste, however, the inherent iron content in the sludge ash (SSA) impedes the P availability of the fused calcium magnesium phosphate fertilizer (FCMP). To achieve the goal of iron removal during the process, carbothermal reduction was adopted for the first time and the performance of carbon addition was systematically

This study investigated the molecular mechanism behind the highly efficient performance of nitrogen-doped carbon dots (NCDs)-assisted microbial electrosynthesis systems (MESs). The impact of NCDs (C:N precursor = 1:0.5–1:3) on acetogens was examined in the biocathode. The highest electrocatalytic performance was observed with NCDs. The maximum acetate production rate of 1.9 ± 0.1 mM d was achieved

Production of functional biocompounds from microalgae has garnered interest from different industrial sectors; however, their overall productivity must be substantially improved for commercialization. Herein, long-term acclimation of was conducted using glucose as an organic carbon source to enhance its heterotrophic capabilities and the production potential of loliolide. A year-long acclimation on

This paper presents an inverse design methodology that utilizes artificial intelligence (AI)-driven experiments to optimize the chemoenzymatic epoxidation of soyabean oil using hydrogen peroxide and lipase (Novozym 435). First, experiments are conducted using a systematic 3-level, 5-factor Box-Behnken design to explore the effect of input parameters on oxirane oxygen content ( (%)). Based on these

Microalgae as a promising approach for wastewater treatment, has challenges in directly treating digested piggery wastewater (DPW) with high ammonia nitrogen (NH-N) concentration. To improve the performance of microalgae in DPW treatment, straw was employed as a substrate to form a straw-microalgae biofilm. The results demonstrated that the straw-microalgae biofilm achieved the highest NH-N removal

The cyclohexane organic acid 3-dehydroshikimate (DHS) has potent antioxidant activity and is widely utilised in chemical and pharmaceutical industries. However, its production requires a long fermentation with a suboptimal yield and low productivity, and a disproportionate growth-to-production ratio impedes the upscaling of DHS synthesis in microbial cell factories. To overcome these limitations, competing

Photosynthetic biohybrid systems (PBSs) composed of semiconductor-microbial hybrids provide a novel approach for converting light into chemical energy. However, comprehending the intricate interactions between materials and microbes that lead to PBSs with high apparent quantum yields (AQY) is challenging. Machine learning holds promise in predicting these interactions. To address this issue, this study

Sophorose is the most effective inducer for cellulase production by . Currently, the biosynthesis of sophorose is very inefficient, resulting in that unavailable for cellulase production in industry. In this study, CoGH1A, a multifunctional thermophilic glycoside hydrolase, was employed for sophorose production. Under the optimized conditions, the sophorose yield was 37.86 g/L with a productivity of

N-acetyl-D-glucosamine and its dimer are degradation products of chitin waste with great potential in therapeutic and agricultural applications. However, the hydrolysis of insoluble chitin by chitinases remains a major bottleneck. This study investigated the biochemical properties and catalytic mechanisms of PoChi chitinase obtained from with a focus on enhancing its efficiency during the degradation

A self-corrosion microelectrolysis (SME)-enhanced membrane-aerated biofilm reactor (eMABR) was developed for the removal of pollutants and reduction of antibiotic resistance genes (ARGs). Fe and Fe formed iron oxides on the biofilm, which enhanced the adsorption and redox process. SME can induce microorganisms to secrete more extracellular proteins and up-regulate the expression of ammonia monooxygenase

Biodiesel serves as a crucial biofuel alternative to petroleum-based diesel fuels, achieved through enzymatic transesterification of oil substrates. This study aims to investigate stabilized lipase (LP) within calcium carbonate (CaCO) microparticles as a catalyst for solvent-free transesterification in biodiesel synthesis. The specific hydrolysis activity of the immobilized LP was 66% of that of free

Dynamic transformation of dissolved organic matter (DOM) contributes to short-chain fatty acids (SCFAs) production during anaerobic digestion. However, the impact of refined transformation of DOM ranked by molecular weight (MW) on SCFAs has never been investigated. Results indicated that DOM conversion order was 3500–7000 Da>(MW>14000 Da) > 7000–4000 Da during hydrolysis stage, while it was independent

In the current study, a novel heterogeneous catalyst has been prepared from waste coconut trunk biomass using an ultrasound-assisted batch reactor. It is observed from the characterization studies that the raw coconut trunk biomass consists of the maximum amount of silicon dioxide (SiO) present in it which is further converted to mullite (composition of 3AlO.2SiO) with a composition of 94.18 % (analyzed

This study investigates the performance, resilience and microbial community dynamics of two anaerobic processes, i.e. pure anammox (R1) and partial denitrification/anammox (PD/A) (R2), following a 30-day starvation period. The tolerance to starvation was assessed by comparing nitrogen removal efficiency and microbial activity across both reactors. Results show that the PD/A process recovery to pre-starvation

Microbial cell factories provide an efficient approach for the green manufacturing of chemicals. However, the excessive use of sugars increases the potential risk of food crisis. Methanol, an abundant feedstock, holds promise in facilitating low-carbon production processes. However, the current methanol bioconversion is hindered by limited regulatory strategies and relatively low conversion efficiency

The increasing production of industrial solid waste requires better disposal solutions. Porous hollow microspheres (PHM) are small inorganic materials with high surface area and adsorption capacity, but their potential for use in anaerobic digestion (AD) has not been explored. With PHM as additive, the effects of different industrial solid wastes (waste glass, steel slag, and fly ash) with different

Anaerobic ammonia oxidation (Anammox) exhibits promise for wastewater treatment,but the enrichment of anammox bacteria (AnAOB) in municipal wastewater treatment plants is a significant challenge. This study constructed a novel noxic-aerobic-xic (AAnO) process with a pure biofilm anoxic zone fed with actual fluctuating municipal wastewater and operated for six months to enrich AnAOB at ambient temperature

An integrated process of sludge in-situ fermentation, biological phosphorus removal and endogenous denitrification (ISFPR-ED) was developed to treat low ratio of chemical oxygen demand to nitrogen (COD/N) wastewater and waste activated sludge (WAS) in a single reactor. Nutrient removal and WAS reduction were achieved due to -dominated sludge fermentation provided organic carbon in extending the anaerobic

Insect-based protein production has gained traction in recent years. This has led to the increasing production of frass, the residual substrate from insect farming. As a relatively new substrate with characteristics that are not widely known, its energetic potential still needs to be investigated. In this context, this literature review aims to evaluate the potential of frass as a feedstock for bioenergy

A single-stage partial nitrification/anammox (PN/A) system with biocarriers was used to treat the permeate from an anaerobic membrane reactor (AnMBR) processing organic fraction of municipal solid wastes. The suitable Ca/P ratio and high pH in the AnMBR permeate facilitated hydroxyapatite (HAP) formation, enhancing the biofilm attachment and the settleability of suspended sludge. This maintained sufficient

The present research employs a pilot-scale selective electrodialysis system to treat liquid digestate, fractionating nutrient ions and exploring fertilizer creation via ammonia stripping and phosphorus precipitation, while studying pharmaceutical transport behavior and examining membrane fouling. The influence of diverse potentials was studied in simulated and real digestate, with 30 V application

This study is the first to explore the performance and mechanism of biochar-impacted sediment microbial fuel cell for removing antibiotic resistance genes (ARGs), and examines the effects of different biochar contents. The addition of 5% biochar produced the highest output voltage and power density, which increased by 100% and 219%, respectively, while simultaneously reducing the abundance and risk

The bioleaching utilizing indigenous microbial inoculation can continuously improve the dewaterability of sludge. In this study, metagenomic analysis was innovative employed to identify the key microorganisms and functional genes that affect the dewatering performance of sludge in the bioleaching conditioning process. The results demonstrated that long-term repeated inoculation of acidified sludge

Due to the serious threat posed by tebuconazole to the aquatic ecosystem, it is imperative to develop a highly efficient adsorbent material for the sustainable remediation of tebuconazole-contaminated water. Herein, a phosphorus (P)-doped biochar from corn straw and HPO was fabricated by one-step pyrolysis for tebuconazole adsorption. Results showed that the P-doped biochar produced at 500℃ (PBC500)

Production of medium chain fatty acids (MCFAs) from CO through microbial electrosynthesis (MES) holds great potential. The present study investigated the effect of cathode voltages of − 0.8 V (MES-1), −1.0 V (MES-2) and −1.2 V vs Ag/AgCl (MES-3), on the production of MCFAs from CO and ethanol using an enriched culture. Direct current (DC) power supply was used to maintain constant cathode voltages

Composting faces challenges with nitrogen (N) losses through ammonia (NH) and nitrous oxide (NO) emissions. In this study, wood vinegar (WV) and biochar (BC) were applied individually or combined into wheat straw and chicken manure composting. Results showed that BC and WV reduced NH volatilizations by 22–23 % individually, but their combined application achieved a 59 % reduction. However, this combination

Methylpyridines are a class of highly toxic pyridine derivatives. In this study, a novel degrading bacterium was isolated for 3-methylpyridine (3-MP) degradation ( ZJJ, GenBank accession NO. OP430847.1; CCTCC M 2022975). The maximum specific degradation rate, half-saturation constant and inhibition constant were fitted to be 0.48 h, 88.3 mg L and 924.0 mg L, respectively. During 3-MP biodegradation

Microbial electrosynthesis for CO utilization (MESCU) producing valuable chemicals with high energy density has garnered attention due to its long-term stability and high coulombic efficiency. The data-driven approaches offer a promising avenue by leveraging existing data to uncover the underlying patterns. This comprehensive review firstly uncovered the potentials of utilizing data-driven approaches

Initiating aerobic fermentation under low temperature is the main challenge for winter livestock manure composting. This study aims to address this issue by applying black soldier fly larvae (BSFL) frass as a co-composting additive to enhance the low-temperature composting process. Specifically, this work explored the effects of chicken manure and BSFL frass co-composting on the temperature, humus

Sludge pretreatment plays a crucial role in solubilizing particulate matters to release organic matter for subsequent anaerobic fermentation (AF). This study innovatively combines radio frequency (RF) heating and alkaline treatment, and finds that the combined pretreatment achieved a sludge disintegration rate of 35.11 %, which is 15.19 % and 8.48 % higher than single RF or alkaline pretreatment. The

The production of alternative proteins is of great significance in the mitigation of food problems. This study proposes an integrated approach including protein extraction, enzymatic hydrolysis, and fermentation to produce both plant proteins and single-cell proteins as alternative proteins from tobacco leaves, a highly-abundant and protein-rich agricultural waste. Alkaline extraction of proteins before

By utilizing the conformational selectivity of biosynthesis and the flexibility of chemical synthesis, researchers have formulated metabolic engineering-based semi-synthetic approaches that initiate with the final product’s structure and identify key biosynthesis intermediates. Nonetheless, these tailored semi-synthetic routes focused on end-products, neglecting the possibility of biobased intermediates

Microbial electrosynthesis (MES) shows great promise for converting CO into high-value chemicals. However, cathode biofilm erosion by high CO sparging and the unclear role of plankton in MES hinders the continuous improvement of its performance. This study aims to enhance biofilm resistance and improve interactions between bio-cathode and plankton by upgrading waste algal biomass into 3-D porous algal

The aerobic granular sludge (AGS) process had been extensively studied for its simultaneous nitrification and denitrification (SND) capabilities. Iron-carbon (IC) had enhanced AGS nitrogen removal efficiency, but the mechanism remained unclear. In this study, four reactors had been added with 50, 30, 10, and 0 g/L of IC. Total nitrogen removal efficiency increased with IC dosage under the same operation