This study investigated the combination of low-thermal and sodium dodecylbenzene sulfonate (SDBS) as a pretreatment method to improve the volatile fatty acids (VFA) production from waste activated sludge (WAS). The results showed that the maximum VFA yield of 320 ± 7.7 mg COD/g VS was obtained in the combined pretreatment (0.01 g SDBS/g TS + 70 ℃ for 60 min), which was 1.8, 1.7 and 4.0 times of that

To study the effects of citric acid on fermentation process of Amomum villosum silage, A. villosum was ensiled without or with 1%, 2% citric acid and fermentation parameters and bacterial diversity were analyzed after 3, 7, 14, 30 days ensiling, respectively. Citric acid treated silages had lower dry matter loss (1.83% vs 2.23%), pH (3.84 vs 6.02), ammonia-N (0.33 vs 1.79 g/kg DM) and coliform bacteria

A new bioprocess has been developed that allows for producing β-carotene from the xylose portion of bioenergy sorghum. Bioenergy sorghum was pretreated in a pilot-scale continuous hydrothermal reactor followed by disc refining. Xylose was extracted using low-severity dilute acid hydrolysis. A xylose yield of 64.9% (17.4 g/L) was obtained by hydrolyzing at 120°C for 5 min with 2% sulfuric acid. The

The coexistence of nitrate and antibiotics in wastewater is a common problem. The study aimed to explore response of denitrifying community, denitrification genes and antibiotic resistance genes (ARGs) to oxytetracycline (OTC) stress in polycaprolactone (PCL) supported solid-phase denitrification (SPD) reactors. Complete nitrate reduction (> 99%) was achieved in SPD system with OTC stress of 0, 0.05

The performance of nitrogen and organic carbon removal in a single reactor (R1) operating with A. faecalis strain NR aerobically was assessed. Under 150 mg/L influent NH4+-N, 91.3%, 71.4% and 90.9% of NH4+-N, TN and TOC were removed, presenting much higher efficiency than a control bioreactor inoculating activated sludge (R0). The amoA gene expression from strain NR in R1 was 7.8 times higher than

Water activity in biotechnological applications plays a vital role. Estimation and precise control of water activity will enhance the product. The objective here is to use digital image processing technique in MATLAB for the quantification of the biomass and water condensate on a horizontal surface. A model has been developed with the data produced experimentally in this study in abiotic conditions

Improving the content and production of high-value ketocarotenoid pigments is critical for the commercialization of microalgal biorefineries. This study reported the use of magnesium aminoclay (MgAC) nanoparticles for enhancement of astaxanthin production by Haematococcus pluvialis in photoautotrophic cultures. Addition of 1.0 g/L MgAC significantly promoted cellular astaxanthin biosynthesis (302±69

Lactic acid (LA) fermentation was conducted with suspended and immobilized cells of an isolated Lactobacillus plantarum 23 strain using various fermentation strategies. Glucose and an alternative, relatively inexpensive carbon source - the hydrolysate of microalga Chlorella vulgaris ESP-31, were used as the carbon source. Batch fermentation using immobilized cells of L. plantarum 23 could enhance LA

Nanoparticles and antibiotics, the two most frequently detected emerging pollutants from different wastewater sources, are eventually discharged into wastewater treatment plants. In this study, the widely used materials CuNPs and oxytetracycline (OTC) were selected as target pollutants to investigate their joint effects on anaerobic ammonium oxidation (anammox). The results indicated that the environmental

In the present investigation, recovery of malic acid from the fermentation broth was performed by using in situ reactive extraction method employing different combination of amine and solvent systems. Totally six solvent mixtures were tested for toxicity on Aspergillus niger PJR1. Further, effect of solvent mixture concentration on separation of malic acid was investigated. Solvent system consisting

Sewage can become a valuable source if its treatment is re-oriented for recovery. An anaerobic forward osmosis membrane bioreactor (AnOMBR) was developed for real municipal sewage treatment to investigate performance, biogas production, flux change and mixed liquor characteristics. The AnOMBR had a good treatment capacity with removal ratio of chemical oxygen demand, ammonia nitrogen, total nitrogen

Two Lytic polysaccharide Mono-Oxygenases (LPMOs), non-modular (PMO_08942) and modular (PMO_07920), from thermotolerant fungus Aspergillus terreus 9DR cloned and expressed in Pichia pastoris X33 and purified to homogeneity using ion-exchange chromatography were found to be of ∼29 and ∼40 kDa, respectively. Both LPMOs were optimally active at 50° C; PMO_08942 was active under acidic condition (pH 5.0)

A mutant library of the key amino acid residue site E387 in γ-glutamyltranspeptidase was constructed to screen the mutant enzymes with significantly improved thermal stability (E387Q). The reaction temperature of the mutant enzyme (E387Q) was 10℃ higher than that of the parent enzyme. Ultrasound-assisted synthesis of L-theanine by γ-glutamyltranspeptidase was investigated. The effects of ultrasonic

A single helical baffle (SHB), consisting of twisted turns, was developed to convert straight flow into spiral flow in a Chlorella PY-ZU1 open raceway pond (ORWP) bubbled with 15% CO2. Microalgal solution flowing through the SHB alternative helical interspaces generated whirling flow both vertically and horizontally, which decreased mixing and increased mass transfer rates. The optimized SHB had a

This study investigated whether bioaugmentation can improve the anaerobic digestion (AD) performance of food waste (FW), as well as the effects of addition dosage and frequency on the bioaugmentation’s performance and economic feasibility. The findings demonstrated that all the bioaugmented digesters, regardless of dosage and frequency, performed more effectively in biogas production than the non-bioaugmentation

The experiments on bio-oil recovery in a vertical tubular condenser with two flumes were conducted for speculating the componential distribution of walnut shell pyrolysis vapors during condensation. Bio-oil elements and functional groups from different locations of condenser were compared with each other. Aromatic H and H in phenolic OH were concentrated in the top and middle bio-oil and their percentage

The macronutrients ratio present in tannery wastes is normally not ideal for anaerobic digestion (AD). In most cases, it is necessary to add nutrients to obtain a more balanced AD process and to ensure favorable conditions for the growth and metabolism of microorganisms. The aim of this study was to verify the influence of the components of nutrient solution added to AD of tannery wastes with regard

In this study, the biological decolorization of reactive black 5 (RB5) by Klebsiella sp. KL-1 in yeast extract (YE) medium was captured the recolorization after exposure to O2, which induced a 15.82% reduction in decolorization efficiency. Similar result was also observed in YE+lactose medium, but not in YE+glucose/xylose media (groups YE+Glu/Xyl). Through biodegradation studies, several degradation

The present research explored the optimization of volatile fatty acids (VFA) production from cheese whey in an anaerobic sequencing batch reactor (AnSBR). For that purpose, changes of solid and hydraulic retention times (SRT and HRT) were applied. Moreover, the experiments were coupled to metagenomic analyses by 16S rRNA sequencing. Results showed an enhancement of process effectiveness at longer SRT

Two grades of recycled wood (Waste Wood A and Waste Wood B) were fractionated on a pilot scale (800 BD kg) to monomeric sugars, lignin and lignosulfonates using SO2-Ethanol-Water (AVAP®) technology including pretreatment, separation of cellulosic and hemicellulosic streams, and saccharification. Carbohydrate mass balance was obtained through determination of poly-, oligo- and monosaccharides as well

Oleaginous microorganisms are among the most promising alternative sources of lipids for oleochemicals and biofuels. However, in the course of lipid production, reactive oxygen species (ROS) are generated inevitably as byproducts of aerobic metabolisms. Although excessive accumulation of ROS leads to lipid peroxidation, DNA damage, and protein denaturation, ROS accumulation has been suggested to enhance

Flotation is a key step during mineral separation. Xanthates are the most commonly used collectors for recovering Cu, Ni, and Zn from sulphide ores. However, xanthates are fossil-based and toxic for the environment. The aim of this study was to evaluate the use of lignin nanoparticles and microparticles as sustainable and environmentally friendly collectors. Lignin particles demonstrated good selectivity

The inclusion of S0 hydrolysis in a kinetic model of autotrophic denitrification has been recently proposed; however the model has not been calibrated or validated yet. Thus, a new methodology was developed and applied to calibrate and validate this kinetic model for the first time. An inoculum adapted from a poultry wastewater treatment plant at stoichiometric S0/NO3- ratio was used. The model was

Lignocellulosic waste has offered a cost-effective and food security-wise substrate for the generation of biofuels and value-added products. However, its recalcitrant properties necessitate pretreatment. Of the various pretreatment methods, alkaline techniques have gained prominence as efficient catalysts. The kraft pulping industry represents a major hub for the generation of white, black and green