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Fine mapping of qAHPS07 and functional studies of AhRUVBL2 controlling pod size in peanut (Arachis hypogaea L.) Plant Biotech. J. (IF 13.263) Pub Date : 2023-05-31 Hui Yang, Lu Luo, Yuying Li, Huadong Li, Xiurong Zhang, Kun Zhang, Suqing Zhu, Xuanlin Li, Yingjie Li, Yongshan Wan, Fengzhen Liu
Cultivated peanut (Arachis hypogaea L.) is an important oil and cash crop. Pod size is one of the major traits determining yield and commodity characteristic of peanut. Fine mapping of quantitative trait locus (QTL) and identification of candidate genes associated with pod size are essential for genetic improvement and molecular breeding of peanut varieties. In this study, a major QTL related to pod
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Genome editing of the susceptibility gene ZmNANMT confers multiple disease resistance without agronomic penalty in maize Plant Biotech. J. (IF 13.263) Pub Date : 2023-05-30 Ya-Jie Li, Jin-Ming Gu, Shijun Ma, Yang Xu, Mengjie Liu, Chuang Zhang, Xiangguo Liu, Guan-Feng Wang
Maize is one of the most important crops in the world. However, great yield losses have been caused by several major diseases worldwide, including southern leaf blight (SLB), northern leaf blight (NLB) and Fusarium stalk rot (FSR) (Mueller et al., 2020). Identification of resistance genes and cultivation of genetic materials conferring multiple disease resistance (MDR) is one of the most effective
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Engineered gamma radiation phytosensors for environmental monitoring Plant Biotech. J. (IF 13.263) Pub Date : 2023-05-24 Robert G. Sears, Stephen B. Rigoulot, Alessandro Occhialini, Britany Morgan, Tayebeh Kakeshpour, Holly Brabazon, Caitlin N. Barnes, Erin M. Seaberry, Brianna Jacobs, Chandler Brown, Yongil Yang, Tayler M. Schimel, Scott C. Lenaghan, C. Neal Stewart
Nuclear energy, already a practical solution for supplying energy on a scale similar to fossil fuels, will likely increase its footprint over the next several decades to meet current climate goals. Gamma radiation is produced during fission in existing nuclear reactors and thus the need to detect leakage from nuclear plants, and effects of such leakage on ecosystems will likely also increase. At present
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Homeostasis of flavonoids and triterpenoids most likely modulates starch metabolism for pollen tube penetration in rice Plant Biotech. J. (IF 13.263) Pub Date : 2023-05-23 Hua-Mao Wu, Dong-Jiang Xie, Peng-Fei Jia, Zuo-Shun Tang, Dong-Qiao Shi, Guang-Hou Shui, Guo-Dong Wang, Wei-Cai Yang
In angiosperms, the timely delivery of sperm cell nuclei by pollen tube (PT) to the ovule is vital for double fertilization. Penetration of PT into maternal stigma tissue is a critical step for sperm cell nuclei delivery, yet little is known about the process. Here, a male-specific and sporophytic mutant xt6, where PTs are able to germinate but unable to penetrate the stigma tissue, is reported in
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Rice OsGA2ox9 regulates seed GA metabolism and dormancy Plant Biotech. J. (IF 13.263) Pub Date : 2023-05-23 Mei-Qing Xing, Su-Hui Chen, Xiao-Fan Zhang, Hong-Wei Xue
Seed development is important for grain yield and quality and finely controlled by complex regulatory networks involving various factors. Phytohormone gibberellin (GA) plays an essential role in late embryogenesis of Arabidopsis and GA metabolism and signalling-related genes are preferentially expressed in rice embryo. Considering the effect in promoting seed germination, how to ensure that GA will
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Low temperature compensates for defective tapetum initiation to restore the fertility of the novel TGMS line ostms15 Plant Biotech. J. (IF 13.263) Pub Date : 2023-05-19 Yu Han, Sheng-Zhe Jiang, Xiang Zhong, Xing Chen, Chang-Kai Ma, Yan-Ming Yang, Yi-Chen Mao, Si-Da Zhou, Lei Zhou, Yan-Fei Zhang, Xue-Hui Huang, Hui Zhang, Lai-Geng Li, Jun Zhu, Zhong-Nan Yang
In rice breeding, thermosensitive genic male sterility (TGMS) lines based on the tms5 locus have been extensively employed. Here, we reported a novel rice TGMS line ostms15 (Oryza sativa ssp. japonica ZH11) which show male sterility under high temperature and fertility under low temperature. Field evaluation from 2018 to 2021 revealed that its sterility under high temperature is more stable than that
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The RUBY reporter enables efficient haploid identification in maize and tomato Plant Biotech. J. (IF 13.263) Pub Date : 2023-05-17 Dong Wang, Yu Zhong, Bin Feng, Xiaolong Qi, Tongzheng Yan, Jinchu Liu, Shuwei Guo, Yuwen Wang, Zongkai Liu, Dehe Cheng, Yuling Zhang, Yunlu Shi, Shuaisong Zhang, RuXue Pan, Chenxu Liu, Shaojiang Chen
In vivo haploid induction has been extended from maize to monocotyledonous plants like rice, wheat, millet and dicotyledonous plants such as tomato, rapeseed, tobacco and cabbage. Accurate identification of haploids is a crucial step of doubled haploid technology, where a useful identification marker is very pivotal. R1-nj is an extensively used visual marker for haploid identification in maize. RFP
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The AP2/ERF transcription factor TOE4b regulates photoperiodic flowering and grain yield per plant in soybean Plant Biotech. J. (IF 13.263) Pub Date : 2023-05-12 Haiyang Li, Haiping Du, Zerong Huang, Milan He, Lingping Kong, Chao Fang, Liyu Chen, Hui Yang, Yuhang Zhang, Baohui Liu, Fanjiang Kong, Xiaohui Zhao
Photoperiod-mediated flowering determines the phenological adaptability of crops including soybean (Glycine max). A genome-wide association study (GWAS) identified a new flowering time locus, Time of flowering 13 (Tof13), which defined a gene encoding an AP2/ERF transcription factor. This new transcription factor, which we named TOE4b, is localized in the nucleus. TOE4b has been selected for soybean
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A systematic dissection in oilseed rape provides insights into the genetic architecture and molecular mechanism of yield heterosis Plant Biotech. J. (IF 13.263) Pub Date : 2023-05-11 Jiang Ye, Huabing Liang, Xueyang Zhao, Na Li, Dongji Song, Jiepeng Zhan, Xinfa Wang, Jinxing Tu, Rajeev Kumar Varshney, Jiaqin Shi, Hanzhong Wang
Heterosis refers to the better performance of cross progeny compared with inbred parents, and its utilization contributes greatly to agricultural production. Several hypotheses have been proposed to explain heterosis mainly including dominance, over-dominance (or pseudo-overdominance) and epistasis. However, systematic dissection and verification of these hypotheses are rarely documented. Here, comparison
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A dramatic decline in fruit citrate induced by mutagenesis of a NAC transcription factor, AcNAC1 Plant Biotech. J. (IF 13.263) Pub Date : 2023-05-10 Bei-ling Fu, Wen-qiu Wang, Xiang Li, Tong-hui Qi, Qiu-fang Shen, Kun-feng Li, Xiao-fen Liu, Shao-jia Li, Andrew C. Allan, Xue-ren Yin
Citrate is a common primary metabolite which often characterizes fruit flavour. The key regulators of citrate accumulation in fruit and vegetables are poorly understood. We systematically analysed the dynamic profiles of organic acid components during the development of kiwifruit (Actinidia spp.). Citrate continuously accumulated so that it became the predominate contributor to total acidity at harvest
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Identification of two novel rice S genes through combination of association and transcription analyses with gene-editing technology Plant Biotech. J. (IF 13.263) Pub Date : 2023-05-08 Yuchen Xu, Lu Bai, Minghao Liu, Yanchen Liu, Shasha Peng, Pei Hu, Dan Wang, Qi Liu, Shuangyong Yan, Lijun Gao, Xuli Wang, Yuese Ning, Shimin Zuo, Wenjing Zheng, Shiming Liu, Wensheng Xiang, Guo-Liang Wang, Houxiang Kang
Traditional rice blast resistance breeding largely depends on utilizing typical resistance (R) genes. However, the lack of durable R genes has prompted rice breeders to find new resistance resources. Susceptibility (S) genes are potential new targets for resistance genetic engineering using genome-editing technologies, but identifying them is still challenging. Here, through the integration of genome-wide
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Metabolic engineering and mechanical investigation of enhanced plant autoluminescence Plant Biotech. J. (IF 13.263) Pub Date : 2023-05-08 Peng Zheng, Jieyu Ge, Jiayi Ji, Jingling Zhong, Hongyu Chen, Daren Luo, Wei Li, Bo Bi, Yongjun Ma, Wanghui Tong, Leiqin Han, Siqi Ma, Yuqi Zhang, Jianping Wu, Yanqiu Zhao, Ronghui Pan, Pengxiang Fan, Mengzhu Lu, Hao Du
The fungal bioluminescence pathway (FBP) was identified from glowing fungi, which releases self-sustained visible green luminescence. However, weak bioluminescence limits the potential application of the bioluminescence system. Here, we screened and characterized a C3′H1 (4-coumaroyl shikimate/quinate 3′-hydroxylase) gene from Brassica napus, which efficiently converts p-coumaroyl shikimate to caffeic
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Genomic analyses provide insights into the polyploidization-driven herbicide adaptation in Leptochloa weeds Plant Biotech. J. (IF 13.263) Pub Date : 2023-05-08 Ke Chen, Haona Yang, Yajun Peng, Ducai Liu, Jingyuan Zhang, Zhenghong Zhao, Lamei Wu, Tao Lin, Lianyang Bai, Lifeng Wang
Polyploidy confers a selective advantage under stress conditions; however, whether polyploidization mediates enhanced herbicide adaptation remains largely unknown. Tetraploid Leptochloa chinensis is a notorious weed in the rice ecosystem, causing severe yield loss in rice. In China, L. chinensis has only one sister species, the diploid L. panicea, whose damage is rarely reported. To gain insights into
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Comparative transcriptome profiling reveals the multiple levels of crosstalk in phytohormone networks in Brassica napus Plant Biotech. J. (IF 13.263) Pub Date : 2023-05-08 Dongxu Liu, Guanbo Yan, Shengbo Wang, Liangqian Yu, Wei Lin, Shaoping Lu, Liang Guo, Qing-Yong Yang, Cheng Dai
Plant hormones are the intrinsic factors that control plant development. The integration of different phytohormone pathways in a complex network of synergistic, antagonistic and additive interactions has been elucidated in model plants. However, the systemic level of transcriptional responses to hormone crosstalk in Brassica napus is largely unknown. Here, we present an in-depth temporal-resolution
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The ubiquitin-binding protein MdRAD23D1 mediates drought response by regulating degradation of the proline-rich protein MdPRP6 in apple (Malus domestica) Plant Biotech. J. (IF 13.263) Pub Date : 2023-05-04 Xiao-Li Zhang, Xiao-Qing Gong, Xin-Jian Su, Hai-Xia Yu, Si-Yuan Cheng, Jing-Wen Huang, Dan-Yang Li, Zhao-Long Lei, Ming-jun Li, Feng-wang Ma
RAD23 (RADIATION SENSITIVE23) proteins are a group of UBL-UBA (ubiquitin-like-ubiquitin-associated) proteins that shuttle ubiquitylated proteins to the 26S proteasome for breakdown. Drought stress is a major environmental constraint that limits plant growth and production, but whether RAD23 proteins are involved in this process is unclear. Here, we demonstrated that a shuttle protein, MdRAD23D1, mediated
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High-efficiency prime editing enables new strategies for broad-spectrum resistance to bacterial blight of rice Plant Biotech. J. (IF 13.263) Pub Date : 2023-05-03 Ajay Gupta, Bo Liu, Qi-Jun Chen, Bing Yang
Using genetic resistance against bacterial blight (BB) caused by Xanthomonas oryzae pathovar oryzae (Xoo) is a major objective in rice breeding programmes. Prime editing (PE) has the potential to create novel germplasm against Xoo. Here, we use an improved prime-editing system to implement two new strategies for BB resistance. Knock-in of TAL effector binding elements (EBE) derived from the BB susceptible
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Developing a UV–visible reporter-assisted CRISPR/Cas9 gene editing system to alter flowering time in Chrysanthemum indicum Plant Biotech. J. (IF 13.263) Pub Date : 2023-05-01 Lei Liu, Yujin Xue, Jiayi Luo, Mingzheng Han, Xuening Liu, Tianhua Jiang, Yafei Zhao, Yanjie Xu, Chao Ma
Chrysanthemum (Chrysanthemum morifolium Ramat.) is an economically important ornamental crop worldwide. This typical obligate short-day (SD) herbaceous perennial in the Asteraceae (Compositae) family is a useful model for studying the photoperiodic control of flowering. However, the complex, heterozygous chrysanthemum genome and the self-incompatibility of this species have hindered basic and applied
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Creating high-resistant starch rice by simultaneous editing of SS3a and SS3b Plant Biotech. J. (IF 13.263) Pub Date : 2023-05-01 Lichun Huang, Ying Xiao, Wei Zhao, Yanan Rao, Huimin Shen, Zhengwen Gu, Xiaolei Fan, Qianfeng Li, Changquan Zhang, Qiaoquan Liu
The conventional starch from cereals is usually high in energy and easily digested, absorbed and converted into blood sugar in human small intestine, while resistant starch (RS) is hardly enzymatically hydrolysed into glucose in human small intestine and only fermented into beneficial short-chain fatty acids in large intestine (Jukanti et al., 2020; Shen et al., 2022). Thus, RS-rich foods can not only
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Evolution-guided multiomics provide insights into the strengthening of bioactive flavone biosynthesis in medicinal pummelo Plant Biotech. J. (IF 13.263) Pub Date : 2023-04-28 Weikang Zheng, Wang Zhang, Dahui Liu, Minqiang Yin, Xia Wang, Shouchuang Wang, Shuangqian Shen, Shengjun Liu, Yue Huang, Xinxin Li, Qian Zhao, Lu Yan, Yuantao Xu, Shiqi Yu, Bin Hu, Tao Yuan, Zhinan Mei, Lanping Guo, Jie Luo, Xiuxin Deng, Qiang Xu, Luqi Huang, Zhaocheng Ma
Pummelo (Citrus maxima or Citrus grandis) is a basic species and an important type for breeding in Citrus. Pummelo is used not only for fresh consumption but also for medicinal purposes. However, the molecular basis of medicinal traits is unclear. Here, compared with wild citrus species/Citrus-related genera, the content of 43 bioactive metabolites and their derivatives increased in the pummelo. Furthermore
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Rice ubiquitin-conjugating enzyme OsUbc13 negatively regulates immunity against pathogens by enhancing the activity of OsSnRK1a Plant Biotech. J. (IF 13.263) Pub Date : 2023-04-27 Jianping Liu, Bo Nie, Boling Yu, Feiyun Xu, Qian Zhang, Ya Wang, Weifeng Xu
Ubc13 is required for Lys63-linked polyubiquitination and innate immune responses in mammals, but its functions in plant immunity still remain largely unknown. Here, we used molecular biological, pathological, biochemical, and genetic approaches to evaluate the roles of rice OsUbc13 in response to pathogens. The OsUbc13-RNA interference (RNAi) lines with lesion mimic phenotypes displayed a significant
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Loss of OsHRC function confers blast resistance without yield penalty in rice Plant Biotech. J. (IF 13.263) Pub Date : 2023-04-27 Yanpeng Ding, Fuping Zhang, Fangyao Sun, Jilu Liu, Zhenzhen Zhu, Xi He, Guihua Bai, Zhongfu Ni, Qixin Sun, Zhenqi Su
Rice blast, caused by Magnaporthe oryzae, is a serious disease in rice (Oryzae sativa L.) worldwide. Because the pathogen mutates rapidly, rice blast has become one of the major threats for global rice production (Wang and Valent, 2017). Using host resistance is the most effective method for blast control. However most rice resistance genes are race-specific (Li et al., 2017). Discovering race non-specific
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Design of high-monounsaturated fatty acid soybean seed oil using GmPDCTs knockout via a CRISPR-Cas9 system Plant Biotech. J. (IF 13.263) Pub Date : 2023-04-21 Haibo Li, Runnan Zhou, Peiyan Liu, Mingliang Yang, Dawei Xin, Chunyan Liu, Zhanguo Zhang, Xiaoxia Wu, Qingshan Chen, Ying Zhao
Soybeans (Glycine max (L.) Merr.) are critical resources for vegetable oil production for human and animal consumption as well as biofuel synthesis. Soybean oils are primarily composed of triacylglycerols (TAGs) with a certain mixture of fatty acid moieties. In general, the composition is approximately 62% polyunsaturated fatty acids (PUFAs), 23% monounsaturated fatty acids (MUFAs) and 15% saturated
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Chromosome-scale genome assembly-assisted identification of Mi-9 gene in Solanum arcanum accession LA2157, conferring heat-stable resistance to Meloidogyne incognita Plant Biotech. J. (IF 13.263) Pub Date : 2023-04-19 Lijun Jiang, Jian Ling, Jianlong Zhao, Yu Yang, Yuhong Yang, Yan Li, Yang Jiao, Zhenchuan Mao, Yunsheng Wang, Bingyan Xie
Root-knot nematodes (RKNs) are infamous plant pathogens in tomato production, causing considerable losses in agriculture worldwide. Mi-1 is the only commercially available RKN-resistance gene; however, the resistance is inactivated when the soil temperature is over 28 °C. Mi-9 in wild tomato (Solanum arcanum LA2157) has stable resistance to RKNs under high temperature but has not been cloned and applied
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The engineered CRISPR-Mb2Cas12a variant enables sensitive and fast nucleic acid-based pathogens diagnostics in the field Plant Biotech. J. (IF 13.263) Pub Date : 2023-04-17 Jian Jiao, Yiqi Liu, Mengli Yang, Jingcheng Zheng, Chonghuai Liu, Wenxiu Ye, Shangwei Song, Tuanhui Bai, Chunhui Song, Miaomiao Wang, Jiangli Shi, Ran Wan, Kunxi Zhang, Pengbo Hao, Jiancan Feng, Xianbo Zheng
Existing CRISPR/Cas12a-based diagnostic platforms offer accurate and vigorous monitoring of nucleic acid targets, but have the potential to be further optimized for more efficient detection. Here, we profiled 16 Cas12a orthologs, focusing on their trans-cleavage activity and their potential as diagnostic enzymes. We observed the Mb2Cas12a has more robust trans-cleavage activity than other orthologs
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Divergent selection of KNR6 maximizes grain production by balancing the flowering-time adaptation and ear size in maize Plant Biotech. J. (IF 13.263) Pub Date : 2023-04-16 Weiya Li, Haitao Jia, Manfei Li, Yiqin Huang, Wenkang Chen, Pengfei Yin, Zhixing Yang, Qiuyue Chen, Feng Tian, Zuxin Zhang, Xiaohong Yang, Lei Liu
Domestication and improvement have led to dramatic changes in the plant architecture and inflorescence in maize. These transformations were achieved by selecting desirable alleles for target traits to meet human needs and local environment adaptation, such as grain yield and flowering time (Doebley et al., 2006). Indeed, the selection of KRN2 and KRN4 genes contributed to the enlargement of ear size
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Rice tapetum differentiation is sensitive to downregulation of OsUCH3, a ubiquitin C-terminal hydrolase Plant Biotech. J. (IF 13.263) Pub Date : 2023-04-14 Dong-Hui Wang, Na Liu, Si-Da Ye, Zhi-Shan Chen, Ya-Nan Lin, Zhao-Hui Liu, Zhi-Hong Xu, Shu-Nong Bai
The tapetum is a special tissue within the stamen that supplies nutrients to support microspore differentiation. Many genes have been identified to function in tapetum differentiation (Astrand et al., 2021). Ubiquitin C-terminal hydrolase (UCH) is a type of cysteine protease involved in protein deubiquitination process (Mevissen and Komander, 2017) and plays roles in gonadal transformation and spermatogenesis
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The miR156b–GmSPL2b module mediates male fertility regulation of cytoplasmic male sterility-based restorer line under high-temperature stress in soybean Plant Biotech. J. (IF 13.263) Pub Date : 2023-04-14 Xianlong Ding, Jinfeng Guo, Menglin Lv, Hongjie Wang, Ying Sheng, Ying Liu, Junyi Gai, Shouping Yang
High-temperature (HT) stress at flowering stage causes significant damage to soybean, including pollen abortion and fertilization failure, but few genes involved in male fertility regulation under HT stress in soybean have been characterized. Here, we demonstrated that miR156b–GmSPL2b module involved in male fertility regulation of soybean cytoplasmic male sterility (CMS)-based restorer line under
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Tunable control of insect pheromone biosynthesis in Nicotiana benthamiana Plant Biotech. J. (IF 13.263) Pub Date : 2023-04-09 Kalyani Kallam, Elena Moreno-Giménez, Ruben Mateos-Fernández, Connor Tansley, Silvia Gianoglio, Diego Orzaez, Nicola Patron
Previous work has demonstrated that plants can be used as production platforms for molecules used in health, medicine, and agriculture. Production has been exemplified in both stable transgenic plants and using transient expression strategies. In particular, species of Nicotiana have been engineered to produce a range of useful molecules, including insect sex pheromones, which are valued for species-specific
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Corrigendum Plant Biotech. J. (IF 13.263) Pub Date : 2023-04-11
In the article “Genetic variation in CaTIFY4b contributes to drought adaptation in chickpea” by Barmukh et al. (2022), the authors wish to add a double affiliation to co-author Jana Kholova, and insert an additional funding grant in the Acknowledgements section. The affiliation of Jana Kholova is as follows: 1Crops Physiology & Modeling, Accelerated Crop Improvement Research Theme, International Crops
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Auxin inhibits lignin and cellulose biosynthesis in stone cells of pear fruit via the PbrARF13-PbrNSC-PbrMYB132 transcriptional regulatory cascade Plant Biotech. J. (IF 13.263) Pub Date : 2023-04-09 Shaozhuo Xu, Manyi Sun, Jia-Long Yao, Xiuxia Liu, Yongsong Xue, Guangyan Yang, Rongxiang Zhu, Weitao Jiang, Runze Wang, Cheng Xue, Zhiquan Mao, Jun Wu
Stone cells are often present in pear fruit, and they can seriously affect the fruit quality when present in large numbers. The plant growth regulator NAA, a synthetic auxin, is known to play an active role in fruit development regulation. However, the genetic mechanisms of NAA regulation of stone cell formation are still unclear. Here, we demonstrated that exogenous application of 200 μM NAA reduced
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Phytophthora sojae effector PsAvh113 associates with the soybean transcription factor GmDPB to inhibit catalase-mediated immunity Plant Biotech. J. (IF 13.263) Pub Date : 2023-03-27 Xiaoguo Zhu, Liang Guo, Ruiqing Zhu, Xiaoyi Zhou, Jianing Zhang, Die Li, Shidan He, Yongli Qiao
Phytophthora species are the most destructive plant pathogens worldwide and the main threat to agricultural and natural ecosystems; however, their pathogenic mechanism remains largely unknown. Here, we show that Avh113 effector is required for the virulence of Phytophthora sojae and is important for development of Phytophthora root and stem rot (PRSR) in soybean (Glycine max). Ectopic expression of
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Multiplex CRISPR/Cas9-mediated mutagenesis of alfalfa FLOWERING LOCUS Ta1 (MsFTa1) leads to delayed flowering time with improved forage biomass yield and quality Plant Biotech. J. (IF 13.263) Pub Date : 2023-03-25 Tezera W. Wolabu, Kashif Mahmood, Ivone Torres Jerez, Lili Cong, Jianfei Yun, Michael Udvardi, Million Tadege, Zengyu Wang, Jiangqi Wen
Alfalfa (Medicago sativa L.) is a perennial flowering plant in the legume family that is widely cultivated as a forage crop for its high yield, forage quality and related agricultural and economic benefits. Alfalfa is a photoperiod sensitive long-day (LD) plant that can accomplish its vegetative and reproductive phases in a short period of time. However, rapid flowering can compromise forage biomass
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Rapid production of novel beneficial alleles for improving rice appearance quality by targeting a regulatory element of SLG7 Plant Biotech. J. (IF 13.263) Pub Date : 2023-03-25 Wenchen Tan, Jun Miao, Bo Xu, Chuting Zhou, Yirui Wang, Xueqi Gu, Shuainan Liang, Baoxiang Wang, Chen Chen, Jinyan Zhu, Shimin Zuo, Zefeng Yang, Zhiyun Gong, Aiqing You, Shujun Wu, Guohua Liang, Yong Zhou
Rice, an important food crop consumed by more than half of the world's population, is also a tradeable commodity. The appearance quality of rice, characterized by grain shape, chalkiness, transparency and colour, greatly influences its commercial value. Generally, varieties with slender grains are associated with good appearance quality (Zhao et al., 2022). Several quantitative trait loci (QTL) for
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Production of double haploid watermelon via maternal haploid induction Plant Biotech. J. (IF 13.263) Pub Date : 2023-03-23 Shouwei Tian, Jian Zhang, Hong Zhao, Mei Zong, Maoying Li, Guoyi Gong, Jinfang Wang, Jie Zhang, Yi Ren, Haiying Zhang, Shaofang Li, Changlong Wen, Yong Xu
Watermelon (Citrullus lanatus, 2n = 2x = 22) is an economically important Cucurbitaceae species that is widely cultivated worldwide. Commercial watermelon cultivars are usually F1 hybrids due to harness heterosis, which used pure lines as parents. However, obtaining pure inbred lines requires at least eight generations of self-pollinating, which is time-consuming, resource-intensive and cost-prohibitive
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Comprehensive genomic analyses of Vigna unguiculata provide insights into population differentiation and the genetic basis of key agricultural traits Plant Biotech. J. (IF 13.263) Pub Date : 2023-03-25 Lei Pan, Minghui Liu, Yan Kang, Xiang Mei, Gege Hu, Chun Bao, Yu Zheng, Huixia Zhao, Chanyou Chen, Nian Wang
Vigna unguiculata is an important legume crop worldwide. The subsp. sesquipedalis and unguiculata are the two major types grown; the former is mainly grown in Asia to produce fresh pods, while the latter is mainly grown in Africa to produce seeds. Here, a chromosome-scale genome for subsp. sesquipedalis was generated by combining high-fidelity (HiFi) long-read sequencing with high-throughput chromosome
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Poaceae-specific β-1,3;1,4-d-glucans link jasmonate signalling to OsLecRK1-mediated defence response during rice-brown planthopper interactions Plant Biotech. J. (IF 13.263) Pub Date : 2023-03-23 Yang-Shuo Dai, Di Liu, Wuxiu Guo, Zhi-Xuan Liu, Xue Zhang, Li-Li Shi, De-Mian Zhou, Ling-Na Wang, Kui Kang, Feng-Zhu Wang, Shan-Shan Zhao, Yi-Fang Tan, Tian Hu, Wu Chen, Peng Li, Qing-Ming Zhou, Long-Yu Yuan, Zhenfei Zhang, Yue-Qin Chen, Wen-Qing Zhang, Juan Li, Lu-Jun Yu, Shi Xiao
Brown planthopper (BPH, Nilaparvata lugens), a highly destructive insect pest, poses a serious threat to rice (Oryza sativa) production worldwide. Jasmonates are key phytohormones that regulate plant defences against BPH; however, the molecular link between jasmonates and BPH responses in rice remains largely unknown. Here, we discovered a Poaceae-specific metabolite, mixed-linkage β-1,3;1,4-d-glucan
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N6-methyladenosine RNA modification regulates cotton drought response in a Ca2+ and ABA-dependent manner Plant Biotech. J. (IF 13.263) Pub Date : 2023-03-22 Baoqi Li, Mengmeng Zhang, Weinan Sun, Dandan Yue, Yizan Ma, Boyang Zhang, Lingfeng Duan, Maojun Wang, Keith Lindsey, Xinhui Nie, Xianlong Zhang, Xiyan Yang
N6-methyladenosine (m6A) is the most prevalent internal modification present in mRNAs, and is considered to participate in a range of developmental and biological processes. Drought response is highly regulated at the genomic, transcriptional and post-transcriptional levels. However, the biological function and regulatory mechanism of m6A modification in the drought stress response is still poorly
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Engineering microalgae for water phosphorus recovery to close the phosphorus cycle Plant Biotech. J. (IF 13.263) Pub Date : 2023-03-15 Long Wang, Xianqing Jia, Lei Xu, Jiahong Yu, Suna Ren, Yujie Yang, Kaibin Wang, Damar López-Arredondo, Luis Herrera-Estrella, Hans Lambers, Keke Yi
As a finite and non-renewable resource, phosphorus (P) is essential to all life and crucial for crop growth and food production. The boosted agricultural use and associated loss of P to the aquatic environment are increasing environmental pollution, harming ecosystems, and threatening future global food security. Thus, recovering and reusing P from water bodies is urgently needed to close the P cycle
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Nucleotide-binding leucine-rich repeat network underlies nonhost resistance of pepper against the Irish potato famine pathogen Phytophthora infestans Plant Biotech. J. (IF 13.263) Pub Date : 2023-03-13 Soohyun Oh, Sejun Kim, Hyo-Jeong oPark, Myung-Shin Kim, Min-Ki Seo, Chih-Hang Wu, Hyun-Ah Lee, Hyun-Soon Kim, Sophien Kamoun, Doil Choi
Nonhost resistance (NHR) is a robust plant immune response against non-adapted pathogens. A number of nucleotide-binding leucine-rich repeat (NLR) proteins that recognize non-adapted pathogens have been identified, although the underlying molecular mechanisms driving robustness of NHR are still unknown. Here, we screened 57 effectors of the potato late blight pathogen Phytophthora infestans in nonhost
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Depletion of the NbCORE receptor drastically improves agroinfiltration productivity in older Nicotiana benthamiana plants Plant Biotech. J. (IF 13.263) Pub Date : 2023-03-14 Isobel Dodds, Changlong Chen, Pierre Buscaill, Renier A. L. van der Hoorn
Nicotiana benthamiana is increasingly used for transient gene expression to produce antibodies, vaccines, and other pharmaceutical proteins but transient gene expression is low in fully developed, 6–8-week old plants. This low gene expression is thought to be caused by the perception of the cold shock protein (CSP) of Agrobacterium tumefaciens. The CSP receptor is contested because both NbCSPR and
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Single-cell transcriptome atlas identified novel regulators for pigment gland morphogenesis in cotton Plant Biotech. J. (IF 13.263) Pub Date : 2023-03-10 Lu Long, Fu-Chun Xu, Chun-Hu Wang, Xiao-Tong Zhao, Man Yuan, Chun-Peng Song, Wei Gao
Cotton (Gossypium spp.) is a leading economic crop that is grown in more than 50 countries. The cottonseeds, once regarded as the by-product of fibre production, contain a rich supply of unsaturated fatty acids, proteins and vitamins. To date, the annual production of cottonseeds has the potential to meet the protein requirements for 550 million people globally, which shows great potential as a food
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Killer to cure: Expression and production costs calculation of tobacco plant-made cancer-immune checkpoint inhibitors Plant Biotech. J. (IF 13.263) Pub Date : 2023-02-21 Laura A. Ridgley, Nicole Falci Finardi, Benjamin B. Gengenbach, Patrick Opdensteinen, Zack Croxford, Julian K.-C. Ma, Mark Bodman-Smith, Johannes F. Buyel, Audrey Y.-H. Teh
Immune checkpoint inhibitors (ICIs) have achieved huge clinical success. However, many still have limited response rates, and are prohibitively costly. There is a need for effective and affordable ICIs, as well as local manufacturing capacity to improve accessibility, especially to low-to-middle income countries (LMICs). Here, we have successfully expressed three key ICIs (anti-PD-1 Nivolumab, anti-NKG2A
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Deep haplotype analyses of target-site resistance locus ACCase in blackgrass enabled by pool-based amplicon sequencing Plant Biotech. J. (IF 13.263) Pub Date : 2023-02-18 Sonja Kersten, Fernando A. Rabanal, Johannes Herrmann, Martin Hess, Zev N. Kronenberg, Karl Schmid, Detlef Weigel
Rapid adaptation of weeds to herbicide applications in agriculture through resistance development is a widespread phenomenon. In particular, the grass Alopecurus myosuroides is an extremely problematic weed in cereal crops with the potential to manifest resistance in only a few generations. Target-site resistances (TSRs), with their strong phenotypic response, play an important role in this rapid adaptive
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HSP90.2 promotes CO2 assimilation rate, grain weight and yield in wheat Plant Biotech. J. (IF 13.263) Pub Date : 2023-02-16 Yan Yan, Meng-Lu Wang, Yue-Ting Guo, Ci-Hang Ding, Ke-Xin Niu, Xiao-Ming Li, Congwei Sun, Zhongdong Dong, Dangqun Cui, Awais Rasheed, Chenyang Hao, Xueyong Zhang, Ganggang Guo, Zhongfu Ni, Qixin Sun, Feng Chen, Jin-Ying Gou(缑金营)
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Faster induction of photosynthesis increases biomass and grain yield in glasshouse-grown transgenic Sorghum bicolor overexpressing Rieske FeS Plant Biotech. J. (IF 13.263) Pub Date : 2023-02-14 Maria Ermakova, Russell Woodford, Zachary Taylor, Robert T. Furbank, Srinivas Belide, Susanne von Caemmerer
Sorghum is one of the most important crops providing food and feed in many of the world's harsher environments. Sorghum utilizes the C4 pathway of photosynthesis in which a biochemical carbon-concentrating mechanism results in high CO2 assimilation rates. Overexpressing the Rieske FeS subunit of the Cytochrome b6f complex was previously shown to increase the rate of photosynthetic electron transport
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LAZY3 interacts with LAZY2 to regulate tiller angle by modulating shoot gravity perception in rice Plant Biotech. J. (IF 13.263) Pub Date : 2023-02-14 Yueyue Cai, Linzhou Huang, Yuqi Song, Yundong Yuan, Shuo Xu, Xueping Wang, Yan Liang, Jie Zhou, Guifu Liu, Jiayang Li, Wenguang Wang, Yonghong Wang
Starch biosynthesis in gravity-sensing tissues of rice shoot determines the magnitude of rice shoot gravitropism and thus tiller angle. However, the molecular mechanism underlying starch biosynthesis in rice gravity-sensing tissues is still unclear. We characterized a novel tiller angle gene LAZY3 (LA3) in rice through map-based cloning. Biochemical, molecular and genetic studies further demonstrated
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Biotinylated Tn5 transposase-mediated CUT&Tag efficiently profiles transcription factor-DNA interactions in plants Plant Biotech. J. (IF 13.263) Pub Date : 2023-02-14 Xiao-Yuan Tao, Xue-Ying Guan, Gao-Jie Hong, Yu-Qing He, Su-Juan Li, Shou-Li Feng, Jian Wang, Guang Chen, Fei Xu, Jia-Wei Wang, Sheng-Chun Xu
In contrast to CUT&Tag approaches for profiling bulk histone modifications, current CUT&Tag methods for analysing specific transcription factor (TF)-DNA interactions remain technically challenging due to TFs having relatively low abundance. Moreover, an efficient CUT&Tag strategy for plant TFs is not yet available. Here, we first applied biotinylated Tn5 transposase-mediated CUT&Tag (B-CUT&Tag) to
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The “humanized” N-glycosylation pathway in CRISPR/Cas9-edited Nicotiana benthamiana significantly enhances the immunogenicity of a S/preS1 Hepatitis B Virus antigen and the virus-neutralizing antibody response in vaccinated mice Plant Biotech. J. (IF 13.263) Pub Date : 2023-02-13 Ana-Maria Pantazica, André van Eerde, Mihaela-Olivia Dobrica, Iuliana Caras, Irina Ionescu, Adriana Costache, Catalin Tucureanu, Hege Steen, Catalin Lazar, Inger Heldal, Sissel Haugslien, Adrian Onu, Crina Stavaru, Norica Branza-Nichita, Jihong Liu Clarke
The recent SARS-CoV-2 pandemic has taught the world a costly lesson about the devastating consequences of viral disease outbreaks but also, the remarkable impact of vaccination in limiting life and economic losses. Vaccination against human Hepatitis B Virus (HBV), a major human pathogen affecting 290 million people worldwide, remains a key action towards viral hepatitis elimination by 2030. To meet
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Protein interactomes for plant lipid biosynthesis and their biotechnological applications Plant Biotech. J. (IF 13.263) Pub Date : 2023-02-10 Yang Xu, Stacy D. Singer, Guanqun Chen
Plant lipids have essential biological roles in plant development and stress responses through their functions in cell membrane formation, energy storage and signalling. Vegetable oil, which is composed mainly of the storage lipid triacylglycerol, also has important applications in food, biofuel and oleochemical industries. Lipid biosynthesis occurs in multiple subcellular compartments and involves
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Field assessment of genome-edited, low asparagine wheat: Europe's first CRISPR wheat field trial Plant Biotech. J. (IF 13.263) Pub Date : 2023-02-09 Sarah Raffan, Joseph Oddy, Andrew Mead, Gary Barker, Tanya Curtis, Sarah Usher, Christopher Burt, Nigel G. Halford
We reported in this journal in 2021 the generation of wheat genotypes in which the asparagine synthetase gene, TaASN2, had been ‘knocked out’ using CRISPR-Cas9 (Raffan et al., 2021). The editing had been achieved by introducing genes encoding the Cas9 nuclease, four guide RNAs (gRNAs) and a Bar marker gene into wheat (Triticum aestivum) cv. Cadenza. Here we report the results of a field trial of Line
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TaTPP-7A positively feedback regulates grain filling and wheat grain yield through T6P-SnRK1 signalling pathway and sugar–ABA interaction Plant Biotech. J. (IF 13.263) Pub Date : 2023-02-08 Hongxia Liu, Xuemei Si, Zhenyu Wang, Liangjing Cao, Lifeng Gao, Xiaolong Zhou, Wenxi Wang, Ke Wang, Chengzhi Jiao, Lei Zhuang, Yunchuan Liu, Jian Hou, Tian Li, Chenyang Hao, Weilong Guo, Jun Liu, Xueyong Zhang
Grain size and filling are two key determinants of grain thousand-kernel weight (TKW) and crop yield, therefore they have undergone strong selection since cereal was domesticated. Genetic dissection of the two traits will improve yield potential in crops. A quantitative trait locus significantly associated with wheat grain TKW was detected on chromosome 7AS flanked by a simple sequence repeat marker
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A novel R2R3-MYB transcription factor FaMYB5 positively regulates anthocyanin and proanthocyanidin biosynthesis in cultivated strawberries (Fragaria × ananassa) Plant Biotech. J. (IF 13.263) Pub Date : 2023-02-08 Leiyu Jiang, Maolan Yue, Yongqiang Liu, Nating Zhang, Yuanxiu Lin, Yunting Zhang, Yan Wang, Mengyao Li, Ya Luo, Yong Zhang, Xiaorong Wang, Qing Chen, Haoru Tang
Flavonoids have a major contribution to the fruit quality in cultivated strawberries and are regulated by MYB, bHLH and WD40 transcriptional factors. We reported here the identification of the FaMYB5, an R2R3-MYB transcription factor, which positively regulated the accumulation of anthocyanins and proanthocyanidins through the trans-activation of the F3'H and LAR. The strawberry FaEGL3 and FaLWD1/FaLWD1-like
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Production of the SARS-CoV-2 receptor-binding domain in stably transformed rice plants for developing country applications Plant Biotech. J. (IF 13.263) Pub Date : 2023-02-05 Andrea Saba-Mayoral, Claudia Rosa, Guillermo Sobrino-Mengual, Victoria Armario-Najera, Paul Christou, Teresa Capell
Most of the 41 approved COVID-19 vaccines and ~220 candidates currently undergoing clinical testing (Basta and Moodie, 2020) are designed around the SARS-CoV-2 spike (S) protein or the receptor-binding domain (RBD) within it, which facilitates virus uptake by binding to angiotensin-converting enzyme 2 (ACE2) on the cell surface. The S protein/RBD is also used as a component of diagnostic kits for the
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Genotyping of DNA pools identifies untapped landraces and genomic regions to develop next-generation varieties Plant Biotech. J. (IF 13.263) Pub Date : 2023-02-05 Mariangela Arca, Brigitte Gouesnard, Tristan Mary-Huard, Marie-Christine Le Paslier, Cyril Bauland, Valérie Combes, Delphine Madur, Alain Charcosset, Stéphane D. Nicolas
Landraces, that is, traditional varieties, have a large diversity that is underexploited in modern breeding. A novel DNA pooling strategy was implemented to identify promising landraces and genomic regions to enlarge the genetic diversity of modern varieties. As proof of concept, DNA pools from 156 American and European maize landraces representing 2340 individuals were genotyped with an SNP array
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Functional analysis of the glutathione S-transferases from Thinopyrum and its derivatives on wheat Fusarium head blight resistance Plant Biotech. J. (IF 13.263) Pub Date : 2023-02-01 Xianrui Guo, Qinghua Shi, Mian Wang, Jing Yuan, Jing Zhang, Jing Wang, Yang Liu, Handong Su, Zhen Wang, Jinbang Li, Cheng Liu, Xingguo Ye, Fangpu Han
Fusarium head blight (FHB) is one of the devastating diseases for wheat production worldwide, which causes significant yield losses and reduces grain quality because of mycotoxins contamination in wheat grains. As wheat relatives, Thinopyrum elongatum and Th. ponticum are important genetic resources that can be used to improve wheat FHB resistance. Using recombinant inbred lines derived from a cross
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OsFTL12, a member of FT-like family, modulates the heading date and plant architecture by florigen repression complex in rice Plant Biotech. J. (IF 13.263) Pub Date : 2023-01-31 Rui Zheng, Xiangbing Meng, Qingliang Hu, Bo Yang, Guicai Cui, Yingying Li, Siju Zhang, Yu Zhang, Xuan Ma, Xiaoguang Song, Shanshan Liang, Yunhai Li, Jiayang Li, Hong Yu, Weijiang Luan
FLOWERING LOCUS T (FT), a florigen in Arabidopsis, plays critical roles in floral transition. Among 13 FT-like members in rice, OsFTL2 (Hd3a) and OsFTL3 (RFT1), two rice homologues of FT, have been well characterized to act as florigens to induce flowering under short-day (SD) and long-day (LD) conditions, respectively, but the functions of other rice FT-like members remain largely unclear. Here, we