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Meta-analysis of the make-up and properties of in vitro models of the healthy and diseased blood-brain barrier. Nat. Biomed. Eng. (IF 26.8) Pub Date : 2024-09-20 James G Shamul,Zhiyuan Wang,Hyeyeon Gong,Wenquan Ou,Alisa M White,Diogo P Moniz-Garcia,Shuo Gu,Alisa Morss Clyne,Alfredo Quiñones-Hinojosa,Xiaoming He
In vitro models of the human blood-brain barrier (BBB) are increasingly used to develop therapeutics that can cross the BBB for treating diseases of the central nervous system. Here we report a meta-analysis of the make-up and properties of transwell and microfluidic models of the healthy BBB and of BBBs in glioblastoma, Alzheimer's disease, Parkinson's disease and inflammatory diseases. We found that
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Biointerface engineering through amalgamation of gene technology and site‐specific growth factor conjugation for efficient osteodifferentiation Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-09-20 Zhenxu Wu, Li Mo, Zongliang Wang, Liangsong Song, Eiry Kobatake, Yoshihiro Ito, Yi Wang, Peibiao Zhang
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Physics‐informed neural networks for biopharmaceutical cultivation processes: Consideration of varying process parameter settings Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-09-19 Niklas Adebar, Sabine Arnold, Liliana M. Herrera, Victor N. Emenike, Thomas Wucherpfennig, Jens Smiatek
We present a new modeling approach for the study and prediction of important process outcomes of biotechnological cultivation processes under the influence of process parameter variations. Our model is based on physics‐informed neural networks (PINNs) in combination with kinetic growth equations. Using Taylor series, multivariate external process parameter variations for important variables such as
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Buffer system improves the removal of host cell protein impurities in monoclonal antibody purification Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-09-19 Dániel Lakatos, Martina Idler, Selina Stibitzky, Jennifer Amann, Jakob Schuschkewitz, Dominik Krayl, Judith Liebau, Jan‐Hendrik Grosch, Erik Arango Gutierrez, Simon Kluters
Polysorbates (PS) are commonly used as stabilizers of biopharmaceuticals such as monoclonal antibodies (mAbs). However, they are prone to chemical and enzymatic degradation. The latter can be caused by residual host cell proteins (HCPs) in the drug substance. Degradation affects the functionality of the PS surfactant which can lead to formation of particles. An increasing number of publications describe
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Culture system for longitudinal monitoring of bone dynamics ex vivo Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-09-19 Esther E.A. Cramer, Kim C.J. Hermsen, Linda M. Kock, Keita Ito, Sandra Hofmann
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Electrobiofabrication of antibody sensor interfaces within a 3D printed device yield rapid and robust electrochemical measurements of titer and glycan structure Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-09-16 Chen-Yu Chen, Dana Motabar, Fauziah Rahma Zakaria, Eunkyoung Kim, Benjamin Wu, Gregory F. Payne, William E. Bentley
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Targeting double-stranded nucleic acids using the λExo–pDNA system Nat. Biotechnol. (IF 33.1) Pub Date : 2024-09-18
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A developmental route to hematopoietic stem cells Nat. Biotechnol. (IF 33.1) Pub Date : 2024-09-18 Adam C. Wilkinson, Marella F. T. R. de Bruijn
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Bacteriophage λ exonuclease and a 5′-phosphorylated DNA guide allow PAM-independent targeting of double-stranded nucleic acids Nat. Biotechnol. (IF 33.1) Pub Date : 2024-09-18 Shengnan Fu, Junjie Li, Jing Chen, Linghao Zhang, Jiajia Liu, Huiyu Liu, Xin Su
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3D printed gelatin methacryloyl hydrogels for perfusion culture of human trabecular meshwork cells and glaucoma studies Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-09-18 Bikram Adhikari, Prasanga Barakoti, Mina B. Pantcheva, Melissa D. Krebs
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Active diagnostic ingredients (ADIs) for PCR: A mini‐bioreactor producing dNTPs with silica immobilized R5‐kinases Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-09-18 Anna R. Bird, Elizabeth A. H. Hall
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3D bioprinting technology and equipment based on microvalve control Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-09-18 Rihui Kang, Jiaxing Wu, Rong Cheng, Meng Li, Luxiao Sang, Hulin Zhang, Shengbo Sang
3D bioprinting technology is widely used in biomedical fields such as tissue regeneration and constructing pathological model. The prevailing printing technique is extrusion‐based bioprinting. In this printing method, the bioink needs to meet both printability and functionality, which are often conflicting requirements. Therefore, this study has developed an innovative microvalve‐based equipment, incorporating
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Clear insight into complex multimodal resins and impurities to overcome recombinant protein purification challenges: A review Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-09-18 Maryam Moazami Goodarzi, Reza Jalalirad
Increasing attention has been paid to the purity of therapeutic proteins imposing extensive costs and challenges to the downstream processing of biopharmaceuticals. One of the efforts, that has been exerted to overcome such limitations, was developing multimodal or mixed‐mode chromatography (MMC) resins for launching selective, orthogonal, non‐affinity purification platforms. Despite relatively extensive
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An experimental census of retrons for DNA production and genome editing Nat. Biotechnol. (IF 33.1) Pub Date : 2024-09-17 Asim G. Khan, Matías Rojas-Montero, Alejandro González-Delgado, Santiago C. Lopez, Rebecca F. Fang, Kate D. Crawford, Seth L. Shipman
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Development of a multigene expression system using 2A peptides in Rhodosporidium toruloides Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-09-17 Xiao Guo, Zhenzhen Bai, Huimin Zhao, Shuobo Shi
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Engineering of heterobifunctional biopolymers for tunable binding and precipitation of Strep-Tag proteins and virus-like nanoparticles Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-09-15 James Tang, Matthew Becker, Abraham Lenhoff, Wilfred Chen
Affinity precipitation is a powerful separation method in that it combines the binding selectivity of affinity chromatography with precipitation of captured biomolecules via phase separation triggered by small changes in the environment, e.g., pH, ionic strength, temperature, light, etc. Elastin-like polypeptides (ELPs) are thermally responsive biopolymers composed of pentapeptide repeats VPGVG that
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The modulation of lung cancer cell motility by Calponin 3 is achieved through its ability to sense and respond to changes in substrate stiffness Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-09-15 Yinxiu Chi, Xianhui Wang, Xiaoyun Shao, Dongliang Zhang, Jingjing Han, Linhong Deng, Liucai Yang, Xuebin Qu
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Non-viral targeted insertion of large payloads into T cells Nat. Biomed. Eng. (IF 26.8) Pub Date : 2024-09-16 Zsuzsanna Izsvák
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Architecting a Transcriptional Repressor-based Genetic Inverter for Tryptophan Derived Pathway Regulation in Escherichia coli. Metab. Eng. (IF 6.8) Pub Date : 2024-09-16 Xinyu Gong,Yuxi Teng,Jianli Zhang,Qi Gan,Ming Song,Ameen Alaraj,Peter Kner,Yajun Yan
Efficient microbial cell factories require intricate and precise metabolic regulations for optimized production, which can be significantly aided by implementing regulatory genetic circuits with versatile functions. However, constructing functionally diverse genetic circuits in host strains is challenging. Especially, functional diversification based on transcriptional repressors has been rarely explored
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Biotech news from around the world Nat. Biotechnol. (IF 33.1) Pub Date : 2024-09-13
Researchers from Kenya’s Egerton University and Makerere University in Uganda begin a pilot program to mass breed desert locusts as a sustainable, protein-rich food source for animals and humans. The locusts are grown in giant domed-shaped, temperature- and humidity-controlled greenhouses equipped with wire gauze cages to stop them escaping. The Locust4Industry project aims to enable industrial exploitation
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FDA approves first IDH-targeted glioma drug Nat. Biotechnol. (IF 33.1) Pub Date : 2024-09-13
Voranigo (vorasidenib), made by French drugmaker Servier Pharmaceuticals, was approved in August by the US Food and Drug Administration. The small-molecule isocitrate dehydrogenase-1 (IDH1) and isocitrate dehydrogenase-2 (IDH2) inhibitor has the go-ahead to treat patients with grade 2 astrocytoma or oligodendroglioma with IDH1 or IDH2 mutations. The approval follows a successful phase 3 trial showing
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Engineered parasite delivers therapeutic proteins to the mouse brain Nat. Biotechnol. (IF 33.1) Pub Date : 2024-09-13 Iris Marchal
Macromolecular drugs have limited applications in the brain because they cannot cross the blood–brain barrier. A parasite that does have the natural capacity to travel to the brain is Toxoplasma gondii. In a study in Nature Microbiology, Bracha et al. make use of this feature, engineering T. gondii to deliver large proteins to the brains of mice. The authors targeted several proteins with different
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Genentech, Sangamo ink Alzheimer’s deal Nat. Biotechnol. (IF 33.1) Pub Date : 2024-09-13
Genentech, part of Roche, has signed an exclusive license with Sangamo Therapeutics to develop genomic treatments for two neurodegenerative diseases. Sangamo will receive $50 million up front and could earn up to $1.9 billion in development and commercial milestone payments plus tiered royalties for its proprietary zinc finger repressors directed to the gene encoding the tau protein, which is involved
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Biogenetic Blooms: it’s microbial, and it’s art Nat. Biotechnol. (IF 33.1) Pub Date : 2024-09-13
Nearly a century later, in a bungalow three blocks from the sun-baked crowds of Rockaway Beach, New York, Karen Ingram meticulously paints portraits of flower blossoms in a Petri dish using a medium of yeast. She calls her series “Biogenetic Blooms”: blue roses, yellow and orange daffodils, red tulips, pink orchids, columbines, snapdragons, irises, black-eyed Susans. The process is surprisingly tricky
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Targeting fatty acid metabolism promotes a healthy vaginal microbiome Nat. Biotechnol. (IF 33.1) Pub Date : 2024-09-13 Iris Marchal
Specific microbial communities in the female genital tract are linked to health outcomes; for example, a microbiome deficient in Lactobacillus is observed in bacterial vaginosis. However, available therapies may not effectively promote the growth of beneficial bacterial species that could prevent the recurrence of disease. Writing in Cell, Zhu et al. propose that targeting unsaturated long-chain fatty
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From Bench to Podcast: Nicoletta Cieri Nat. Biotechnol. (IF 33.1) Pub Date : 2024-09-13
On this episode, our Associate Editor Cláudia Vilhena interviews Nicoletta Cieri, the first author of a great story on how a devised analytic framework can aid in the prevention of post-transplant disease recurrence. Join us as Nicoletta and Cláudia explore the highlights of the story, discuss the future of individualized medicine and touch base on future career opportunities following the postdoc
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Bioremediation Nat. Biotechnol. (IF 33.1) Pub Date : 2024-09-13
Recent patents relating to compositions and methods for the remediation of polluted and contaminated environments.
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US selects first ten drugs for price drops Nat. Biotechnol. (IF 33.1) Pub Date : 2024-09-13
A historic law that negotiates price discounts for Medicare prescription drugs has led to the selection of the first ten drugs to be covered by these negotiations. From January 2026, enrollees will pay between 38% and 79% less for the ten commonly prescribed outpatient drugs. The negotiated prices are expected to save taxpayers billions of dollars. The biopharma industry, however, argues that the prices
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Intestinal organoids with an autologous immune compartment Nat. Biotechnol. (IF 33.1) Pub Date : 2024-09-13 Iris Marchal
Although stem-cell derived organoids are useful tools for studying epithelial functions in health and disease, they generally lack the immune compartment critical for modeling organ-level processes. In a paper published in Nature, Recaldin et al. now integrate tissue-resident memory T (TRM) cells into human intestinal organoids and show how this model can be used to gain insight into drug-induced intestinal
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1H24 biotech job picture Nat. Biotechnol. (IF 33.1) Pub Date : 2024-09-13 Michael Francisco
A semiannual snapshot of job expansions, reductions and availability in the biotech and pharma sectors.
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Enzymes boost ‘rock weathering’ to trap CO2 in soil Nat. Biotechnol. (IF 33.1) Pub Date : 2024-09-13
Spreading powdered basalt on farmland traps CO2 from the atmosphere, a technology that is cheap, requires no huge steel reactors and, with a biotech boost, may even reduce fertilizer use.
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People Nat. Biotechnol. (IF 33.1) Pub Date : 2024-09-13
Recent moves of note in and around the biotech and pharma industries.
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The prevalence of post-NDA drug patents and their relationship to the timing of generic approval Nat. Biotechnol. (IF 33.1) Pub Date : 2024-09-13 Jonathan J. Darrow, Victor Van de Wiele, Beatrice Brown, Aaron S. Kesselheim
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A virtual case competition approach to bridging business acumen and biomedical graduate training Nat. Biotechnol. (IF 33.1) Pub Date : 2024-09-13 Kara M. Donovan, Robert S. Goldsmith, Erica V. Ely, Samridhi Garg, Lloyd P. Ruiz, Mitre Athaiya, Sophia Meyer, Michelle Sydney Smith, Ishtiaq Mawla
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Foundation models build on ChatGPT tech to learn the fundamental language of biology Nat. Biotechnol. (IF 33.1) Pub Date : 2024-09-13
Scientists are using ever more sophisticated AI algorithms trained on vast, unlabeled datasets to develop models that can ‘interpret’ biological data to help guide biomolecule design.
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The Wild West of spike-in normalization Nat. Biotechnol. (IF 33.1) Pub Date : 2024-09-13 Lauren A. Patel, Yuwei Cao, Eric M. Mendenhall, Christopher Benner, Alon Goren
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Elucidating sequence‐function relationships in a template‐independent polymerase to enable novel DNA recording applications Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-09-14 Marija Milisavljevic, Teresa Rojas Rodriguez, Keith E. J. Tyo
Harnessing DNA as a high‐density storage medium for information storage and molecular recording of signals has been of increasing interest in the biotechnology field. Recently, progress in enzymatic DNA synthesis, DNA digital data storage, and DNA‐based molecular recording has been made by leveraging the activity of the template‐independent DNA polymerase, terminal deoxynucleotidyl transferase (TdT)
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Learning what keeps nanomedicines in tumours Nat. Biomed. Eng. (IF 26.8) Pub Date : 2024-09-13 Yifan Wang, Benjamin R. Schrank, Wen Jiang, Betty Y. S. Kim
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In situ formation of biomolecular condensates as intracellular drug reservoirs for augmenting chemotherapy Nat. Biomed. Eng. (IF 26.8) Pub Date : 2024-09-13 Tingxizi Liang, Yuxiang Dong, Irina Cheng, Ping Wen, Fengqin Li, Feng Liu, Qing Wu, En Ren, Peifeng Liu, Hongjun Li, Zhen Gu
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Advances in multi-enzyme co-localization strategies for the construction of microbial cell factory Biotechnol. Adv. (IF 12.1) Pub Date : 2024-09-13 Qi Guo, Yu-Xin Yang, Dong-Xun Li, Xiao-Jun Ji, Na Wu, Yue-Tong Wang, Chao Ye, Tian-Qiong Shi
Biomanufacturing, driven by technologies such as synthetic biology, offers significant potential to advance the bioeconomy and promote sustainable development. It is anticipated to transform traditional manufacturing and become a key industry in future strategies. Cell factories are the core of biomanufacturing. The advancement of synthetic biology and growing market demand have led to the production
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Experimental characterization and prediction of Escherichia coli host cell proteome retention during preparative chromatography Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-09-13 Roxana Disela, Tim Neijenhuis, Olivier Le Bussy, Geoffroy Geldhof, Marieke Klijn, Martin Pabst, Marcel Ottens
Purification of recombinantly produced biopharmaceuticals involves removal of host cell material, such as host cell proteins (HCPs). For lysates of the common expression host Escherichia coli (E. coli) over 1500 unique proteins can be identified. Currently, understanding the behavior of individual HCPs for purification operations, such as preparative chromatography, is limited. Therefore, we aim to
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Recoded gene circuits for multiplexed genetic code expansion Nat. Biotechnol. (IF 33.1) Pub Date : 2024-09-11
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Dedifferentiating myoblasts back into a satellite cell state in vitro Nat. Biotechnol. (IF 33.1) Pub Date : 2024-09-11
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Organoid culture promotes dedifferentiation of mouse myoblasts into stem cells capable of complete muscle regeneration Nat. Biotechnol. (IF 33.1) Pub Date : 2024-09-11 Feodor D. Price, Mark N. Matyas, Andrew R. Gehrke, William Chen, Erica A. Wolin, Kristina M. Holton, Rebecca M. Gibbs, Alice Lee, Pooja S. Singu, Jeffrey S. Sakakeeny, James M. Poteracki, Kelsey Goune, Isabella T. Pfeiffer, Sarah A. Boswell, Peter K. Sorger, Mansi Srivastava, Kathleen Lindahl Pfaff, Emanuela Gussoni, Sean M. Buchanan, Lee L. Rubin
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Efficient genetic code expansion without host genome modifications Nat. Biotechnol. (IF 33.1) Pub Date : 2024-09-11 Alan Costello, Alexander A. Peterson, David L. Lanster, Zhiyi Li, Gavriela D. Carver, Ahmed H. Badran
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Droplet-based single-cell sequencing: Strategies and applications Biotechnol. Adv. (IF 12.1) Pub Date : 2024-09-11 Yuting Shang, Zhengzheng Wang, Liqing Xi, Yantao Wang, Meijing Liu, Ying Feng, Juan Wang, Qingping Wu, Xinran Xiang, Moutong Chen, Yu Ding
Notable advancements in single-cell omics technologies have not only addressed longstanding challenges but also enabled unprecedented studies of cellular heterogeneity with unprecedented resolution and scale. These strides have led to groundbreaking insights into complex biological systems, paving the way for a more profound comprehension of human biology and diseases. The droplet microfluidic technology
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Recent advances in engineering synthetic biomolecular condensates Biotechnol. Adv. (IF 12.1) Pub Date : 2024-09-11 Li Wan, Juntao Ke, Yingying Zhu, Wenli Zhang, Wanmeng Mu
Biomolecular condensates are intriguing entities found within living cells. These structures possess the ability to selectively concentrate specific components through phase separation, thereby playing a crucial role in the spatiotemporal regulation of a wide range of cellular processes and metabolic activities. To date, extensive studies have been dedicated to unraveling the intricate connections
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The biosynthesis of L-phenylalanine-derived compounds by engineered microbes Biotechnol. Adv. (IF 12.1) Pub Date : 2024-09-11 Min Qiu, Jie Jiang, Wankui Jiang, Wenming Zhang, Yujia Jiang, Fengxue Xin, Min Jiang
L-Phenylalanine (L-Phe) is an important aromatic amino acid, which has been widely used in food, health care products, medicine and other fields. Based on the relatively mature microbial biosynthesis process, a variety of -phenylalanine-derived compounds have attracted more and more attentions owing to their extensively potential applications in the fields of food, medicine, spices, cosmetics, and
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Biotechnology and Bioengineering: Volume 121, Number 10, October 2024 Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-09-11
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Biosynthesis of nonnutritive monosaccharide d‐allulose by metabolically engineered Escherichia coli from nutritive disaccharide sucrose Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-09-11 Ling‐Jie Zheng, Wei‐Xiang Chen, Shang‐He Zheng, Irfan Ullah, Hui‐Dong Zheng, Li‐Hai Fan, Qiang Guo
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Cover Image, Volume 121, Number 10, October 2024 Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-09-11 Melanie Maier, Stefan Schneider, Linus Weiss, Simon Fischer, Daniel Lakatos, Joey Studts, Matthias Franzreb
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Production of marine-derived bioactive peptide molecules for industrial applications: A reverse engineering approach Biotechnol. Adv. (IF 12.1) Pub Date : 2024-09-10 Akash J. Surwase, Narsinh L. Thakur
This review examines a wide range of marine microbial-derived bioactive peptide molecules, emphasizing the significance of reverse engineering in their production. The discussion encompasses the advancements in Marine Natural Products (MNPs) bio-manufacturing through the integration of omics-driven microbial engineering and bioinformatics. The distinctive features of non-ribosomally synthesised peptides
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Rational design of short‐chain dehydrogenase/reductase for enantio‐complementary synthesis of chiral 1,2‐diols by successive hydroxymethylation and reduction of aldehydes Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-09-10 Xiu‐Xin Ren, Bing‐Mei Su, Xin‐Qi Xu, Lian Xu, Juan Lin
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Application of microbubble air flotation to harvest Microcystis sp. from agriculture wastewater: The regulation and mechanisms Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-09-10 Jianfeng Ye, Zhihao Zhu, Zhaofeng Song, Huiting Xu, Tianchen Xu, Hui Liu
The harvesting of microalgae is the main bottleneck of its large‐scale biomass production, and seeking an efficient, green, and low‐cost microalgae harvesting technology is one of the urgent problems to be solved. Microbubble air flotation has been proven to be an effective measure, but the mechanisms of microbubbles‐algal cell attachment are still unclear. In this study, microbubble air flotation
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Phage genome engineering with retrons Nat. Biotechnol. (IF 33.1) Pub Date : 2024-09-09 Ilya Osterman, Rotem Sorek
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A pan-cancer dye for solid-tumour screening, resection and wound monitoring via short-wave and near-infrared fluorescence imaging Nat. Biomed. Eng. (IF 26.8) Pub Date : 2024-09-09 Benedict Edward Mc Larney, Ali Yasin Sonay, Elana Apfelbaum, Nermin Mostafa, Sébastien Monette, Dana Goerzen, Nicole Aguirre, Rüdiger M. Exner, Christine Habjan, Elizabeth Isaac, Ngan Bao Phung, Magdalena Skubal, Mijin Kim, Anuja Ogirala, Darren Veach, Daniel A. Heller, Jan Grimm
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Combinatorial iterative method for metabolic engineering of Yarrowia lipolytica: application for betanin biosynthesis. Metab. Eng. (IF 6.8) Pub Date : 2024-09-09 Wei Jiang,Shengbao Wang,Paulo Avila,Tue Sparholt Jørgensen,Zhijie Yang,Irina Borodina
Combinatorial library-based metabolic engineering approaches allow lower cost and faster strain development. We developed a genetic toolbox EXPRESSYALI for combinatorial engineering of the oleaginous yeast Yarrowia lipolytica. The toolbox enables consecutive rounds of engineering, where up to three combinatorially assembled gene expression cassettes can be integrated into each yeast clone per round
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Auto‐transduction in lentiviral vector bioprocessing: A quantitative assessment and a novel inhibition strategy Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-09-08 Thomas Williams‐Fegredo, Lee Davies, Carol Knevelman, James Miskin, Kyriacos Mitrophanous, Qasim A. Rafiq
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Advance computational tools for multiomics data learning Biotechnol. Adv. (IF 12.1) Pub Date : 2024-09-07 Sheikh Mansoor, Saira Hamid, Thai Thanh Tuan, Jong-Eun Park, Yong Suk Chung
The burgeoning field of bioinformatics has seen a surge in computational tools tailored for omics data analysis driven by the heterogeneous and high-dimensional nature of omics data. In biomedical and plant science research multi-omics data has become pivotal for predictive analytics in the era of big data necessitating sophisticated computational methodologies. This review explores a diverse array
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From natural to synthetic: Promoter engineering in yeast expression systems Biotechnol. Adv. (IF 12.1) Pub Date : 2024-09-07 Ly Nguyen, Bernhard Schmelzer, Stuart Wilkinson, Diethard Mattanovich
Synthetic promoters are particularly relevant for application not only in yeast expression systems designed for high-level heterologous protein production but also in other applications such as metabolic engineering, cell biological research, and stage-specific gene expression control. By designing synthetic promoters, researcher can create customized expression systems tailored to specific needs,