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A DNA-free and genotype-independent CRISPR/Cas9 system in soybean Plant Physiol. (IF 6.5) Pub Date : 2024-09-23 Chikako Kuwabara, Ryuji Miki, Nobuyuki Maruyama, Masanori Yasui, Haruyasu Hamada, Yozo Nagira, Yumiko Hirayama, Wataru Ackley, Feng Li, Ryozo Imai, Naoaki Taoka, Tetsuya Yamada
Here, we report a smart genome editing system for soybean (Glycine max) using the in planta bombardment-ribonucleoprotein (iPB-RNP) method without introducing foreign DNA or requiring traditional tissue culture processes such as embryogenesis and organogenesis. Shoot apical meristem (SAM) of embryonic axes was used as the target tissue for genome editing because the SAM in soybean mature seeds has
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A one-way ticket: Wheat roots do not functionally refill xylem emboli following rehydration Plant Physiol. (IF 6.5) Pub Date : 2024-09-19 Beatrice L Harrison Day, Kate M Johnson, Vanessa Tonet, Ibrahim Bourbia, Chris J Blackman, Timothy J Brodribb
Understanding xylem embolism spread in roots is essential for predicting the loss of function across root systems during drought. However, the lasting relevance of root embolism to plant recovery depends on whether roots can refill xylem emboli and resume function after rehydration. Using MicroCT and optical and dye staining methods, we investigated embolism repair in rehydrated intact roots of wheat
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Lysine acetylation regulates the subcellular localization and function of WRKY63. Plant Physiol. (IF 6.5) Pub Date : 2024-09-17 Yuan-Hsin Shih,Fu-Yu Hung,Pei-Yu Lin,Jian-Sheng Chen,Yi-Sheng Cheng,Songguang Yang,Keqiang Wu
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Spatial sugar separation is key to how fast you get old. Plant Physiol. (IF 6.5) Pub Date : 2024-09-17 Ryo Yokoyama
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Tips and rules for easy design of active miPEPs and cPEPs. Plant Physiol. (IF 6.5) Pub Date : 2024-09-17 Mélanie Ormancey,Sabine Tourneur,Cécile Pouzet,Patrice Thuleau,Serge Plaza,Jean-Philippe Combier
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Phosphorylation of the transcription factor SlBIML1 by SlBIN2 kinases delays flowering in tomato Plant Physiol. (IF 6.5) Pub Date : 2024-09-17 Siwei Zhang, Rui Deng, Jianwei Liu, Dan Luo, Miaomiao Hu, Shuhua Huang, Meng Jiang, Jia Du, Tong Jin, Dehai Liu, Yuchao Li, Maqsood Khan, Shufen Wang, Xiaofeng Wang
Brassinosteroids (BRs) are well known for their important role in the regulation of plant growth and development. Plants with deficiency in BR signaling show delayed plant development and exhibit late flowering phenotypes. However, the precise mechanisms involved in this process require investigation. In this study, we cloned homologs of BRASSINOSTEROID INSENSITIVE 2 (SlBIN2), the GSK3-like protein
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The transcriptional integration of environmental cues with root cell type development Plant Physiol. (IF 6.5) Pub Date : 2024-09-17 Mona Gouran, Siobhan M Brady
Plant roots navigate the soil ecosystem with each cell type uniquely responding to environmental stimuli. Below ground, the plant's response to its surroundings is orchestrated at the cellular level, including morphological and molecular adaptations that shape root system architecture as well as tissue and organ functionality. Our understanding of the transcriptional responses at cell type resolution
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Developmental responses of roots to limited phosphate availability: research progress and application in cereals Plant Physiol. (IF 6.5) Pub Date : 2024-09-17 Hong Lu, Meiyan Ren, Rongbin Lin, Kangming Jin, Chuanzao Mao
Phosphorus (P), an essential macronutrient, is crucial for plant growth and development. However, available inorganic phosphate (Pi) is often scarce in soil, and its limited mobility exacerbates P deficiency in plants. Plants have developed complex mechanisms to adapt to Pi-limited soils. The root, the primary interface of the plant with soil, plays an essential role in plant adaptation to Pi-limited
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Histone deacetylase GhHDA5 negatively regulates Verticillium wilt resistance in cotton Plant Physiol. (IF 6.5) Pub Date : 2024-09-14 Chunyan Wei, Chaofan Wang, Xin Zhang, Weiyi Huang, Minghui Xing, Chunyan Han, Cangbao Lei, Youpeng Zhang, Xiangyu Zhang, Kai Cheng, Xiao Zhang
Verticillium wilt (VW) caused by Verticillium dahliae (V. dahliae) is one of the most destructive diseases in cotton (Gossypium spp.). Histone acetylation plays critical roles in plant development and adaptive responses to biotic and abiotic stresses. However, the relevance of histone acetylation in cotton VW resistance remains largely unclear. Here, we identified Histone Deacetylase 5 (GhHDA5) from
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Regulatory Network of the Late-Recruited Primary Decarboxylase C4NADP-ME in Sugarcane Plant Physiol. (IF 6.5) Pub Date : 2024-09-14 Xiuting Hua, Huihong Shi, Gui Zhuang, Yuhong Lan, Shaoli Zhou, Dongxu Zhao, Ming-Ju Amy Lyu, Sehrish Akbar, Jia Liu, Yuan Yuan, Zhen Li, Qing Jiang, Kaixin Huang, Yating Zhang, Qing Zhang, Gang Wang, Yu Wang, Xiaomin Yu, Pinghua Li, Xingtan Zhang, Jianping Wang, Shenghua Xiao, Wei Yao, Ray Ming, Xinguang Zhu, MuQing Zhang, Haibao Tang, Jisen Zhang
In agronomically important C4 grasses, efficient CO2 delivery to Rubisco is facilitated by NADP-malic enzyme (C4NADP-ME), which decarboxylates malate in bundle sheath cells. However, understanding the molecular regulation of the C4NADP-ME gene in sugarcane (Saccharum spp.) is hindered by its complex genetic background. Enzymatic activity assays demonstrated that decarboxylation in sugarcane Saccharum
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A leucine-rich-repeat receptor-like kinase regulates pollen aperture formation in rice Plant Physiol. (IF 6.5) Pub Date : 2024-09-14 Xu Zhang, Yu-Jin Kim, Qian Tan, Ki-Hong Jung, Wanqi Liang
Apertures in pollen grains exhibit species-specific patterns and provide an ideal model for studying cell-surface patterning. Pollen apertures are critical for cereal crop fertility, and while DEFECTIVE IN APERTURE FORMATION1 (OsDAF1) and INAPERTURATE POLLEN1 (OsINP1) have been documented to participate in pollen aperture formation in rice (Oryza sativa), the molecular transduction pathway regulating
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Hydraulic mechanism of limiting growth and maintaining survival of desert shrubs in arid habitats Plant Physiol. (IF 6.5) Pub Date : 2024-09-13 Jianqiang Huo, Chengyi Li, Yang Zhao, Gaoling Han, Xinrong Li, Zhishan Zhang
The growth and survival of woody plant species is mainly driven by evolutionary and environmental factors. However, little is known about the hydraulic mechanisms that respond to growth limitation and enable desert shrub survival in arid habitats. To shed light on these hydraulic mechanisms, 9-, 31-, and 56-year-old Caragana korshinskii plants that had been grown under different soil water conditions
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The transcription factors GmVOZ1A and GmWRI1a synergistically regulate oil biosynthesis in soybean Plant Physiol. (IF 6.5) Pub Date : 2024-09-13 Mingming Yang, Changhuan Du, Meng Li, Yuanzhuo Wang, Gege Bao, Jinxiu Huang, Qingyan Zhang, Shuzhen Zhang, Pengfei Xu, Weili Teng, Qingqing Li, Shanshan Liu, Bo Song, Qiang Yang, Zhikun Wang
Soybean [Glycine max (L.) Merr.] is a major oil-producing crop worldwide. Although several related proteins regulating soybean oil accumulation have been reported, little is known about the regulatory mechanisms. In this study, we characterized vascular plant one-zinc-finger 1A (GmVOZ1A) that interacts with WRINKLED 1a (GmWRI1a) using yeast two-hybrid library screening. The GmVOZ1A–GmWRI1a interaction
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Zinc finger protein LjRSDL regulates arbuscule degeneration of arbuscular mycorrhizal fungi in Lotus japonicus Plant Physiol. (IF 6.5) Pub Date : 2024-09-13 Yunjian Xu, Fang Liu, Fulang Wu, Ruifan Zou, Manli Zhao, Jianping Wu, Beijiu Cheng, Xiaoyu Li
In arbuscular mycorrhizal (AM) symbiosis, appropriate regulation of the formation, maintenance, and degeneration of the arbuscule are essential for plants and fungi. In this study, we identified a Cysteine-2/Histidine-2 zinc finger protein (C2H2-ZFP)-encoding gene in Lotus japonicus named Regulator of Symbiosome Differentiation-Like (LjRSDL) that is required for arbuscule degeneration. Evolutionary
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Efficient gene editing of a model fern species through gametophyte-based transformation Plant Physiol. (IF 6.5) Pub Date : 2024-09-13 Wei Jiang, Fenglin Deng, Mohammad Babla, Chen Chen, Dongmei Yang, Tao Tong, Yuan Qin, Guang Chen, Blaine Marchant, Pamela Soltis, Douglas Edward Soltis, Fanrong Zeng, Zhong-Hua Chen
The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated nuclease (Cas) system allows precise and easy editing of genes in many plant species. However, this system has not yet been applied to any fern species through gametophytes due to the complex characteristics of fern genomes, genetics, and physiology. Here, we established a protocol for gametophyte-based screening
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A DUF21 domain-containing protein regulates plant dwarfing in watermelon Plant Physiol. (IF 6.5) Pub Date : 2024-09-13 Piaoyun Sun, Hongjiao Zhao, Lihong Cao, Tian Zhang, Helong Zhang, Tongwen Yang, Bosi Zhao, Yanxin Jiang, Junyang Dong, Tianrui Chen, Biao Jiang, Zheng Li, Junjun Shen
Dwarf or semi-dwarf plant structures are well-suited for intensive farming, maximizing yield, and minimizing labor costs. Watermelon (Citrullus lanatus) is classified as an annual vine plant with elongated internodes, yet the mechanism governing watermelon dwarfing remains unclear. In this study, a compact watermelon mutant dwarf, induced by the insertion of T-DNA, was discovered. Through re-sequencing
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Linking timing to nitrogen use efficiency: rice OsEC-Ghd7-ARE1 module works on it. Plant Physiol. (IF 6.5) Pub Date : 2024-09-12 Munkhtsetseg Tsednee
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Guardians of the light: The redox regulation of the photosystem I during photosynthesis. Plant Physiol. (IF 6.5) Pub Date : 2024-09-12 Sara Selma
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The cytoskeleton controls membrane protein movement. Plant Physiol. (IF 6.5) Pub Date : 2024-09-11 Nicola Trozzi,Alicja B Kunkowska
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VvbHLH036, a basic helix–loop–helix transcription factor regulates the cold tolerance of grapevine Plant Physiol. (IF 6.5) Pub Date : 2024-09-11 Yujun Hou, Darren C J Wong, Xiaoming Sun, Qingyun Li, Huimin Zhou, Lin Meng, Xiaoli Liao, Zhenchang Liang, Rishi Aryal, Qingfeng Wang, Haiping Xin
Cold stress is an adverse environmental factor that limits the growth and productivity of horticulture crops such as grapes (Vitis vinifera). In this study, we identified a grapevine cold-induced basic helix–loop–helix (bHLH) transcription factor (VvbHLH036). Overexpression and CRISPR/Cas9-mediated knockout (KO) of VvbHLH036 enhanced and decreased cold tolerance in grapevine roots, respectively. Transcriptome
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Heterozygous fasciated ear mutations improve yield traits in inbred and hybrid maize lines Plant Physiol. (IF 6.5) Pub Date : 2024-09-11 Jinbiao Wang, Qi Zheng, Ruizhong Zhang, Zhaoyu Huang, Qingyu Wu, Lei Liu, Qiang Ning, David Jackson, Fang Xu
Heterozygous mutations in two genes encoding key regulators of development improve kernel row number in inbred and hybrid maize, revealing their potential for yield improvement.
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How coat proteins shape autophagy in plant cells Plant Physiol. (IF 6.5) Pub Date : 2024-09-11 Taijoon Chung, Ye Eun Choi, Kyoungjun Song, Hyera Jung
Autophagy is a membrane trafficking pathway through which eukaryotic cells target their own cytoplasmic constituents for degradation in the lytic compartment. Proper biogenesis of autophagic organelles requires a conserved set of autophagy-related (ATG) proteins and their interacting factors, such as signalling phospholipid phosphatidylinositol 3-phosphate (PI3P) and coat complex II (COPII). The COPII
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Navigating the shadows: SlCPK10 mediated flower abscission in tomatoes under low light. Plant Physiol. (IF 6.5) Pub Date : 2024-09-10 Prateek Jain
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Transcriptional corepressors in maize maintain meristem development Plant Physiol. (IF 6.5) Pub Date : 2024-09-10 Jason Gregory, Xue Liu, Zongliang Chen, Cecilia Gallardo, Jason Punskovsky, Gabriel Koslow, Mary Galli, Andrea Gallavotti
The formation of the plant body proceeds in a sequential post-embryonic manner through the action of meristems. Tightly coordinated meristem regulation is required for development and reproductive success, eventually determining yield in crop species. In maize (Zea mays), the RAMOSA1 ENHANCER LOCUS2 (REL2) family of transcriptional corepressors includes four members, REL2, RELK1 (REL2-LIKE1), RELK2
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Aquaporin CmPIP2; 3 links H2O2 signal and antioxidation to modulate trehalose-induced cold tolerance in melon seedlings Plant Physiol. (IF 6.5) Pub Date : 2024-09-09 Yuqing Han, Fei Luo, Adan Liang, Dongdong Xu, Hongyi Zhang, Tao Liu, Hongyan Qi
Cold stress severely restricts the growth and development of cold-sensitive crops. Trehalose (Tre), known as the ‘sugar of life’, plays key roles in regulating plant cold tolerance by triggering antioxidation. However, the relevant regulatory mechanism remains unclear. Here, we confirmed that Tre triggers apoplastic hydrogen peroxide (H2O2) production and thus plays key roles in improving the cold
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A hierarchical ubiquitination-mediated regulatory module controls bamboo lignin biosynthesis Plant Physiol. (IF 6.5) Pub Date : 2024-09-09 Kebin Yang, Ziyang Li, Chenglei Zhu, Yan Liu, Hui Li, Xiaolin Di, Xinzhang Song, Haiqing Ren, Zhimin Gao
The lignocellulosic feedstock of woody bamboo shows promising potential as an alternative to conventional wood, attributed to its excellent properties. The content and distribution of lignin serve as the foundation of these properties. While the regulation of lignin biosynthesis in bamboo has been extensively studied at the transcriptional level, its posttranslational control has remained poorly understood
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Chromosome doubling increases PECTIN METHYLESTERASE 2 expression, biomass, and osmotic stress tolerance in kiwifruit Plant Physiol. (IF 6.5) Pub Date : 2024-09-09 Yanyan Zhu, Xinlei Wang, Yan He, Yajing Liu, Runze Wang, Yongsheng Liu, Songhu Wang
Chromosome doubling-induced polyploidization is a popular tool for crop breeding. Polyploidy crops commonly have multiple advantages, including increased biomass and stress tolerance. However, little is known about the genes responsible for these advantages. We found kiwifruit (Actinidia chinensis cv. Hongyang) PECTIN METHYLESTERASE 2 (AcPME2)is substantially upregulated in artificially created tetraploid
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The soybean NUCLEAR FACTOR-Y C4 and α-EXPANSIN 7 module influences phosphorus uptake by regulating root morphology Plant Physiol. (IF 6.5) Pub Date : 2024-09-09 Xiaoqian Liu, Yupeng Cai, Weiwei Yao, Li Chen, Wensheng Hou
Soybean (Glycine max) is a globally important crop; however, its productivity is severely impacted by phosphorus (P) deficiency. Understanding the transcriptional regulation of low P (LP) response mechanisms is essential for enhancing soybean P use efficiency. In this study, we found that the Nuclear Factor-Y (NF-Y) transcription factor GmNF-YC4, in addition to its previously discovered role in regulating
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Transcription factor CpWRKY50 enhances anthracnose resistance by promoting jasmonic acid signaling in papaya Plant Physiol. (IF 6.5) Pub Date : 2024-09-09 Min Yang, Chenping Zhou, Ruibin Kuang, Xiaming Wu, Chuanhe Liu, Han He, Yuerong Wei
Colletotrichum brevisporum is an important fungal pathogen that causes anthracnose and has led to serious postharvest losses of papaya (Carica papaya L.) fruit in recent years. WRKY transcription factors play vital roles in regulating plant resistance to pathogens, but their functions in papaya anthracnose resistance need further exploration. In this study, we identified a WRKY transcription factor
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Bestrophin-like protein 4 is involved in photosynthetic acclimation to light fluctuations in Chlamydomonas Plant Physiol. (IF 6.5) Pub Date : 2024-09-07 Liat Adler, Chun Sing Lau, Kashif M Shaikh, Kim A van Maldegem, Alex L Payne-Dwyer, Cecile Lefoulon, Philipp Girr, Nicky Atkinson, James Barrett, Tom Z Emrich-Mills, Emilija Dukic, Michael R Blatt, Mark C Leake, Gilles Peltier, Cornelia Spetea, Adrien Burlacot, Alistair J McCormick, Luke C M Mackinder, Charlotte E Walker
In many eukaryotic algae, CO2 fixation by Rubisco is enhanced by a CO2-concentrating mechanism, which utilizes a Rubisco-rich organelle called the pyrenoid. The pyrenoid is traversed by a network of thylakoid membranes called pyrenoid tubules, which are proposed to deliver CO2. In the model alga Chlamydomonas (Chlamydomonas reinhardtii), the pyrenoid tubules have been proposed to be tethered to the
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Phosphorylation of auxin signaling repressor IAA8 by heat-responsive MPKs causes defective flower development Plant Physiol. (IF 6.5) Pub Date : 2024-09-07 Sun Ho Kim, Shah Hussain, Huyen Trang Thi Pham, Ulhas Sopanrao Kadam, Sunghwa Bahk, Zakiyah Ramadany, Jeongwoo Lee, Young Hun Song, Kyun Oh Lee, Jong Chan Hong, Woo Sik Chung
Heat stress is a substantial and imminent threat to plant growth and development. Understanding its adverse effects on plant development at the molecular level is crucial for sustainable agriculture. However, the molecular mechanism underlying how heat stress causes developmental defects in flowers remains poorly understood. Here, we identified Indole-3-Acetic Acid 8 (IAA8), a repressor of auxin signaling
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Transcription factors WRKY2 and WRKY34 control LATERAL ORGAN BOUNDARIES DOMAIN10 expression in pollen vegetative cell nuclei Plant Physiol. (IF 6.5) Pub Date : 2024-09-07 Thu-Hien Nguyen, Min Jung Kim, Jungmook Kim
The intricate regulation of gene expression determining cell fate during male gametogenesis involves a complex interplay of multiple transcriptional regulators. In Arabidopsis (Arabidopsis thaliana), the LATERAL ORGAN BOUNDARIES DOMAIN 10 (LBD10) transcription factor is prominent in early microspores and both the germ and vegetative cells of bicellular pollen, playing an important role in pollen development
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COBRA-LIKE4 Modulates Cellulose Synthase Velocity and Facilitates Cellulose Deposition in the Secondary Cell Wall Plant Physiol. (IF 6.5) Pub Date : 2024-09-05 Jan Xue, Grant McNair, Yoichiro Watanabe, Madison Kaplen, Sydne Guevara-Rozo, Mathias Schuetz, Rene Schneider, Shawn D Mansfield, Lacey Samuels
Cellulose is a critical component of secondary cell walls and woody tissues of plants. Cellulose synthase (CESA) complexes (CSCs) produce cellulose as they move within the plasma membrane, extruding glucan chains into the cell wall that coalesce and crystallize into cellulose fibrils. Here we examine COBRA-LIKE4 (COBL4), a GPI-anchored protein on the outer leaflet of the plasma membrane that is required
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The MdIAA29-MdARF4 complex plays an important role in balancing plant height with salt and drought stress responses Plant Physiol. (IF 6.5) Pub Date : 2024-09-05 Yingying Lei, Cui Chen, Wenjun Chen, Hongyan Dai
Breeding dwarf apple (Malus domestica) varieties is a recent trend in agriculture because such varieties are easy to maintain and have high yields; however, dwarf apple trees generally have poor stress tolerance. Balancing apple plant height and stress response has been an important breeding goal. In this study, aux/indole-3-acetic acid 29 gene in apple (MdIAA29) overexpression lines (#1, #2, #3) had
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Advantage looping: Gene regulatory circuits between microRNAs and their target transcription factors in plants Plant Physiol. (IF 6.5) Pub Date : 2024-09-05 Naveen Shankar, Utpal Nath
The 20-24 nucleotide microRNAs (miRNAs) and their target transcription factors (TF) have emerged as key regulators of diverse processes in plants, including organ development and environmental resilience. In several instances, the mature miRNAs degrade the TF-encoding transcripts, while their protein products in turn bind to the promoters of the respective miRNA-encoding genes and regulate their expression
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A conserved protein family in mirid bug Riptortus pedestris plays dual roles in regulating plant immunity Plant Physiol. (IF 6.5) Pub Date : 2024-09-05 Jiangxuan Zhou, Zhiyuan Yin, Danyu Shen, Qingsong Zhang, Yujie OYang, Xiaoxi Li, Yurong Ma, Lanping Ding, Yong Pei, Gan Ai, Yumei Dong, Donglei Yang, Yuanchao Wang, Daolong Dou, Ai Xia
The mirid bug (Riptortus pedestris), a major soybean pest, migrates into soybean fields during the pod filling stage and causes staygreen syndrome, which leads to substantial yield losses. The mechanism by which R. pedestris elicits soybean (Glycine max) defenses and counter-defenses remains largely unexplored. In this study, we characterized a protein family from R. pedestris, designated Riptortus
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YELLOW, SERRATED LEAF is essential for cotyledon vein patterning in Arabidopsis Plant Physiol. (IF 6.5) Pub Date : 2024-09-04 Yetao Wang, Yutong Zheng, Yafei Shi, Deyuan Jiang, Qi Kuang, Xiangsheng Ke, Ming Li, Yukun Wang, Xiaohong Yue, Qin Lu, Xin Hou
Venation develops complex patterns within the leaves of angiosperms, and the mechanism of leaf vein patterning remains poorly understood. Here, we report a spontaneous mutant that exhibits yellow serrated leaves and defective cotyledon vein patterning. We mapped and cloned the relevant gene YELLOW, SERRATED LEAF (YSL), a previously unreported gene in plants. YSL interacts with VH1-interacting kinase
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Sphingolipid homeostasis--how do cells know when enough is enough? Implications for plant pathogen responses Plant Physiol. (IF 6.5) Pub Date : 2024-09-02 Edgar B Cahoon, Panya Kim, Tian Xie, Ariadna González Solis, Gongshe Han, Xin Gong, Teresa M Dunn
Sphingolipid homeostatic regulation is important for balancing plant life and death. Plant cells finely tune sphingolipid biosynthesis to ensure sufficient levels to support growth through their basal functions as major components of endomembranes and the plasma membrane. Conversely, accumulation of sphingolipid biosynthetic intermediates, long-chain bases (LCBs) and ceramides, is associated with programmed
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CPK10 regulates low light–induced tomato flower drop downstream of IDL6 in a calcium-dependent manner Plant Physiol. (IF 6.5) Pub Date : 2024-09-02 Xin Fu, Ruizhen Li, Xianfeng Liu, Lina Cheng, Siqi Ge, Sai Wang, Yue Cai, Tong Zhang, Chun-Lin Shi, Sida Meng, Changhua Tan, Cai-Zhong Jiang, Tianlai Li, Mingfang Qi, Tao Xu
Flower drop is a major cause for yield loss in many crops. Previously, we found that the tomato (Solanum lycopersicum) INFLORESCENCE DEFICIENT IN ABSCISSION-Like (SlIDL6) gene contributes to flower drop induced by low light. However, the molecular mechanisms by which SlIDL6 acts as a signal to regulate low light–induced abscission remain unclear. In this study, SlIDL6 was found to elevate cytosolic
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N6-methyladenosine transcriptome-wide profiles of maize kernel development Plant Physiol. (IF 6.5) Pub Date : 2024-09-02 Jia Wen Wu, Guang Ming Zheng, Lin Zhang, Ya Jie Zhao, Ru Yu Yan, Ru Chang Ren, Yi Ming Wei, Kun Peng Li, Xian Sheng Zhang, Xiang Yu Zhao
Maize (Zea mays L.) kernel development is a complex and dynamic process involving cell division and differentiation, into a variety of cell types. Epigenetic modifications, including DNA methylation, play a pivotal role in regulating this process. N6-methyladenosine modification is a universal and dynamic post-transcriptional epigenetic modification that is involved in the regulation of plant development
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C2-domain abscisic acid-related proteins regulate the dynamics of a plasma membrane H+-ATPase in response to alkali stress Plant Physiol. (IF 6.5) Pub Date : 2024-09-01 Ai-Yu Guo, Wen-Qiang Wu, Wen-Cheng Liu, Yuan Zheng, Di Bai, Yan Li, Jie Xie, Siyi Guo, Chun-Peng Song
Arabidopsis (Arabidopsis thaliana) H+-ATPase1 (AHA1), a plasma membrane (PM)-localized H+-ATPase, plays a key role in plant alkali stress tolerance by pumping protons from the cytoplasm to the apoplast. However, its molecular dynamics are poorly understood. We report that many C2-domain ABA-related (CAR) protein family members interact with AHA1 in Arabidopsis. Single or double mutants of CAR1, CAR6
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Non-transformation methods for studying signaling pathways and genes involved in Brassica rapa pollen–stigma interactions Plant Physiol. (IF 6.5) Pub Date : 2024-08-30 Lili Zhang, Xiaoshuang Cui, Lin Yang, Abdul Raziq, Shiya Hao, Weiqing Zeng, Jiabao Huang, Yunyun Cao, Qiaohong Duan
Self-incompatibility (SI) is a mechanism in plants that prevents self-fertilization and promotes out-crossing. SI is also widely utilized in the breeding of Brassicaceae crops. Understanding the regulatory mechanisms of SI is essential but has been greatly restrained in most Brassicaceae crops due to inefficient transformation. Here, we developed methods for studying signaling pathways and genes of
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Periplasmic carbonic anhydrase CAH1 contributes to high inorganic carbon affinity in Chlamydomonas reinhardtii Plant Physiol. (IF 6.5) Pub Date : 2024-08-30 Daisuke Shimamura, Tomoaki Ikeuchi, Ami Matsuda, Yoshinori Tsuji, Hideya Fukuzawa, Keiichi Mochida, Takashi Yamano
Carbonic anhydrase (CA), an enzyme conserved across species, is pivotal in the interconversion of inorganic carbon (Ci; CO2 and HCO3–). Compared to the well-studied intracellular CA, the specific role of extracellular CA in photosynthetic organisms is still not well understood. In the green alga Chlamydomonas (Chlamydomonas reinhardtii), carbonic anhydrase 1 (CAH1), located at the periplasmic space
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The quantitative trait locus GWY10 controls rice grain width and yield Plant Physiol. (IF 6.5) Pub Date : 2024-08-29 Waseem Abbas, Qi Sun, Yana Cui, Abdullah Shalmani, Pengkun Xu, Yawei Fan, Dejian Zhang, Meng-en Wu, Xingxing Li, Yibo Li
A rice grain width and yield quantitative trait locus increases grain yield in near-isogenic lines and gene-edited lines and improves grain appearance and milling quality.
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RESPIRATORY BURST OXIDASE HOMOLOG 5.1 regulates H3K4me3 deposition and transcription after cold priming in cucumber Plant Physiol. (IF 6.5) Pub Date : 2024-08-29 Qinghua Di, Mengdi Zhou, Yansu Li, Yan Yan, Chaoxing He, Jun Wang, Xiaoqin Wang, Xianchang Yu, Mintao Sun
Plants can maintain acquired cold tolerance for a long period after cold priming, even after the resumption of warmer temperatures. However, the transcriptional mechanisms active during the recovery period after cold priming remain unknown. Here, we found that in cucumber (Cucumis sativus), cold priming altered the Histone H3 lysine 4 trimethylation (H3K4me3) signal of sustainably-induced (memory)
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Mining the oil code: new insights behind oil production in Brassica napus. Plant Physiol. (IF 6.5) Pub Date : 2024-08-28 Sebastián R Moreno
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Leaf Gas Film 1 promotes glycerol ester accumulation and formation of a tight root barrier to radial O2 loss in rice Plant Physiol. (IF 6.5) Pub Date : 2024-08-28 Juan de la Cruz Jiménez, Siti Noorrohmah, Kiran Suresh, Viktoria V Zeisler-Diehl, Lucas León Peralta Ogorek, Max Herzog, Elisa Pellegrini, Keisuke Nagai, Motoyuki Ashikari, Hirokazu Takahashi, Ole Pedersen, Lukas Schreiber, Mikio Nakazono
Rice (Oryza sativa L.) and many other wetland plants form an apoplastic barrier in the outer parts of the roots to restrict radial O2 loss to the rhizosphere during soil flooding. This barrier facilitates longitudinal internal O2 diffusion via gas-filled tissues from shoot to root apices, enabling root growth in anoxic soils. We tested the hypothesis that Leaf Gas Film 1 (LGF1), which influences leaf
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REPRESSOR OF UV-B PHOTOMORPHOGENESIS proteins target ABA INSENSITIVE 5 for degradation to promote early plant development Plant Physiol. (IF 6.5) Pub Date : 2024-08-28 Deeksha Singh, Oihik Mitra, Kalyan Mahapatra, Akshat Singh Raghuvanshi, Rucha Kulkarni, Sourav Datta
REPRESSOR OF UV-B PHOTOMORPHOGENESIS 1 (RUP1) and RUP2 are WD-40 domain-containing proteins that have been extensively characterized for their role in UV-B signaling. However, the roles of the RUP proteins outside the canonical UV-signaling pathway are less known. Here, we identify that RUP1 and RUP2 play important roles in ABA signaling to regulate seed germination and early seedling development in
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Allele-specific Expression of AP2-like ABA repressor 1 Regulates Iron Uptake by Modulating Rhizosphere pH in Apple Plant Physiol. (IF 6.5) Pub Date : 2024-08-28 Huaying Ma, Mengmeng Fu, Zhen Xu, Zicheng Chu, Ji Tian, Yi Wang, Xinzhong Zhang, Zhenhai Han, Ting Wu
Genetic variation within a species can result in allelic expression for natural selection or breeding efforts. Here, we identified an iron (Fe) deficiency-inducible gene, AP2-like ABA repressor 1 (MdABR1), in apple (Malus domestica). MdABR1 exhibited differential expression at the allelic level (MdABR131A and MdABR131G) in response to Fe deficiency. The W-box insertion in the promoter of MdABR131A
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Variation in Q10 of night-time leaf respiratory CO2 efflux by factors other than measurement temperature. Plant Physiol. (IF 6.5) Pub Date : 2024-08-27 Dan Bruhn
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A lipid synthase maintains metabolic flux for jasmonate synthesis to regulate root growth and phosphate homeostasis Plant Physiol. (IF 6.5) Pub Date : 2024-08-27 Mandavi Pandey, Lokesh Verma, Pawandeep Singh Kohli, Bhagat Singh, Abhijith Kochi, Jitender Giri
Plants require phosphate (Pi) for proper growth and development but often face scarcity of this vital nutrient in the soil. Pi-starvation triggers membrane lipid remodeling to utilize the membrane phospholipid-bound Pi in plants. In this process, phospholipids are replaced by non-Pi-containing galactolipids (MGDG, DGDG) and sulfolipids. The galactolipids ratio (MGDG:DGDG) is suggested to influence
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Cold mediates maize root hair developmental plasticity via epidermis-specific transcriptomic responses Plant Physiol. (IF 6.5) Pub Date : 2024-08-27 Yaping Zhou, Mauritz Leonard Sommer, Annika Meyer, Danning Wang, Alina Klaus, Tyll Stöcker, Caroline Marcon, Heiko Schoof, Georg Haberer, Chris-Carolin Schön, Peng Yu, Frank Hochholdinger
Cold stress during early development limits maize (Zea mays L.) production in temperate zones. Low temperatures restrict root growth and reprogram gene expression. Here, we provide a systematic transcriptomic landscape of maize primary roots, their tissues, and cell types in response to cold stress. The epidermis exhibited a unique transcriptomic cold response, and genes involved in root hair formation
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The effector SJP3 interferes with pistil development by sustaining SHORT VEGETATIVE PHASE 3 expression in jujube Plant Physiol. (IF 6.5) Pub Date : 2024-08-27 Mingsheng Deng, Fuli Ma, Liping Zhai, Xinyue Zhang, Ning Zhang, Yunyan Zheng, Wei Chen, Wenmin Zhou, Kaixue Pang, Junyong Zhou, Qibao Sun, Jun Sun
Jujube witches’ broom (JWB) is a phytoplasma disease that causes severe damage to jujube (Ziziphus jujuba) crops worldwide. Diseased jujube plants show enhanced vegetative growth after floral reversion, including leafy flower structures (phyllody) and the fourth whorl converting into a vegetative shoot. In previous research, secreted JWB protein 3 (SJP3) was identified as an inducer of phyllody. However
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Orchestration of phosphate homeostasis by the ITPK1-type inositol phosphate kinase in the liverwort Marchantia polymorpha Plant Physiol. (IF 6.5) Pub Date : 2024-08-27 Naga Jyothi Pullagurla, Supritam Shome, Guizhen Liu, Henning J Jessen, Debabrata Laha
Land plants have evolved sophisticated sensing mechanisms and signalling pathways to adapt to phosphate-limited environments. While molecular players contributing to these adaptations in flowering plants have been described, how non-vascular bryophytes regulate phosphate (Pi) homeostasis remained largely unknown. In this study, we present findings that both male and female plants of the liverwort Marchantia
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TaCAMTA4 negatively regulates H2O2-dependent wheat leaf rust resistance by activating catalase 1 expression Plant Physiol. (IF 6.5) Pub Date : 2024-08-27 Tianjie Sun, Nan Ma, Yuanyuan Jiao, Qian Wang, Qipeng Wang, Na Liu, Yan Chen, Shengfang Han, Chunyan Hou, Rongna Wang, Dongmei Wang
Leaf rust, caused by Puccinia triticina Erikss. (Pt), is a serious disease threatening wheat (Triticum aestivum L.) production worldwide. Hydrogen peroxide (H2O2) triggered by Pt infection in resistant wheat cultivars cause oxidative damage directly to biomolecules or is activated by calcium signaling and mediates the hypersensitive response. Calmodulin-binding transcriptional activator 4 (TaCAMTA4)
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The Complexity of Volatile Terpene Biosynthesis in Roses: Particular Insights into β-Citronellol Production Plant Physiol. (IF 6.5) Pub Date : 2024-08-26 Hongjie Li, Yueqing Li, Huijun Yan, Tingting Bao, Xiaotong Shan, Jean-Claude Caissard, Liangsheng Zhang, Huiyi Fang, Xue Bai, Jia Zhang, Zhaoxuan Wang, Min Wang, Qian Guan, Ming Cai, Guogui Ning, Xiujuan Jia, Benoît Boachon, Sylvie Baudino, Xiang Gao
The fascinating scent of rose (Rosa genus) flowers has captivated human senses for centuries, making them one of the most popular and widely used floral fragrances. Despite much progress over the last decade, many biochemical pathways responsible for rose scents remain unclear. We analyzed the floral scent compositions from various rose varieties and selected the modern cultivar Rosa hybrida ‘Double
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β-Carotene and its derivatives regulate pollen fertility in tomato Plant Physiol. (IF 6.5) Pub Date : 2024-08-26 Sombir Rao, Franz Joseph O’Hanna, Lily Saar, Abhijit Hazra, Olivia Hullihen, James J Giovannoni, Li Li
β-carotene promotes pollen germination and tube growth via a mechanism that regulates reactive oxygen species homeostasis in tomato.
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Promoting γ-aminobutyric acid accumulation to enhances saline-alkali tolerance in tomato Plant Physiol. (IF 6.5) Pub Date : 2024-08-26 Jingrong Wang, Yong Zhang, Junzheng Wang, Fang Ma, Linyang Wang, Xiangqiang Zhan, Guobin Li, Songshen Hu, Abid Khan, Haoran Dang, Tianlai Li, Xiaohui Hu
Saline-alkali stress is a widely distributed abiotic stress that severely limits plant growth. γ-Aminobutyric acid (GABA) accumulates rapidly in plants under saline-alkali stress, but the underlying molecular mechanisms and associated regulatory networks remain unclear. Here, we report a MYB-like protein, I-box binding factor (SlMYBI), which positively regulates saline-alkali tolerance through induced
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A cyclical marker system enables indefinite series of oligonucleotide-directed gene editing in Chlamydomonas reinhardtii Plant Physiol. (IF 6.5) Pub Date : 2024-08-24 Ian L Ross, Hong Phuong Le, Sabar Budiman, Dake Xiong, Fritz Hemker, Elizabeth A Millen, Melanie Oey, Ben Hankamer
CRISPR/Cas9 gene editing in the model green alga Chlamydomonas reinhardtii relies on the use of selective marker genes to enrich for non-selectable target mutations. This becomes challenging when many sequential modifications are required in a single cell line, as useful markers are limited. Here, we demonstrate a cyclical selection process which only requires a single marker gene to identify an almost
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Vernalization or devernalization? A question about VRT2. Plant Physiol. (IF 6.5) Pub Date : 2024-08-22 Dechang Cao