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Efficient production of l‐glutathione by whole‐cell catalysis with ATP regeneration from adenosine Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-04-17 Siqi Zuo, Jiarun Zhao, Pengfei Zhang, Wenqian Liu, Ke Bi, Peilian Wei, Jiazhang Lian, Zhinan Xu
l‐glutathione (GSH) is an important tripeptide compound with extensive applications in medicine, food additives, and cosmetics industries. In this work, an innovative whole‐cell catalytic strategy was developed to enhance GSH production by combining metabolic engineering of GSH biosynthetic pathways with an adenosine‐based adenosine triphosphate (ATP) regeneration system in Escherichia coli. Concretely
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Biotechnology and Bioengineering: Volume 121, Number 5, May 2024 Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-04-13
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Multivariate data analysis on multisensor measurement for inline process monitoring of adenovirus production in HEK293 cells Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-04-13 Xingge Xu, Omar Farnós, Barbara C. M. F. Paes, Sean Nesdoly, Amine A. Kamen
In the era of Biopharma 4.0, process digitalization fundamentally requires accurate and timely monitoring of critical process parameters (CPPs) and quality attributes. Bioreactor systems are equipped with a variety of sensors to ensure process robustness and product quality. However, during the biphasic production of viral vectors or replication‐competent viruses for gene and cell therapies and vaccination
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Cover Image, Volume 121, Number 5, May 2024 Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-04-13 Klemen Božič, Ajda Sedlar, Špela Kralj, Urh Černigoj, Aleš Štrancar, Rok Sekirnik
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Back Cover Image, Volume 121, Number 5, May 2024 Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-04-13 Yixiao Zhang, Sri Madabhushi, Tiffany Tang, Hassan Raza, David J. Busch, Xi Zhao, James Ormes, Sen Xu, Joseph Moroney, Rubin Jiang, Henry Lin, Ren Liu
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Semiautomated design and soluble expression of a chimeric antigen TbpAB01 from Glaesserella parasuis Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-04-10 Jin‐Ping Chen, Lin Zhou, Jin‐Song Gong, Nan‐Kai Wang, Fen‐Fang Miao, Chang Su, Xin‐Le Gao, Guo‐Qiang Xu, Jin‐Song Shi, Zheng‐Hong Xu
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Establishment of low‐cost production platforms of polyhydroxyalkanoate bioplastics from Halomonas cupida J9 Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-04-08 Siqi Wang, Yujie Liu, Hongfu Guo, Yan Meng, Weini Xiong, Ruihua Liu, Chao Yang
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Machine learning approaches to predict TAS2R receptors for bitterants Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-04-08 Francesco Ferri, Marco Cannariato, Marco Agostino Deriu, Lorenzo Pallante
Bitter taste involves the detection of diverse chemical compounds by a family of G protein‐coupled receptors, known as taste receptor type 2 (TAS2R). It is often linked to toxins and harmful compounds and in particular bitter taste receptors participate in the regulation of glucose homeostasis, modulation of immune and inflammatory responses, and may have implications for various diseases. Human TAS2Rs
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Characterization and engineering of peroxisome targeting sequences for compartmentalization engineering in Pichia pastoris Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-04-03 Cuifang Ye, Haosen Hong, Jucan Gao, Mengxin Li, Yuanwei Gou, Di Gao, Chang Dong, Lei Huang, Zhinan Xu, Jiazhang Lian
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Biotechnology and Bioengineering: Volume 121, Number 4, April 2024 Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-03-31
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Plants as a cost‐effective source for customizable photosynthetic wound dressings: A proof of concept study Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-03-31 Sergio González‐Itier, Miguel Miranda, Rocío Corrales‐Orovio, Constanza Vera, Valentina Veloso‐Giménez, Camila Cárdenas‐Calderón, José Tomás Egaña
Oxygen is essential for tissue regeneration, playing a crucial role in several processes, including cell metabolism and immune response. Therefore, the delivery of oxygen to wounds is an active field of research, and recent studies have highlighted the potential use of photosynthetic biomaterials as alternative oxygenation approach. However, while plants have traditionally been used to enhance tissue
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Expression of filaments of the Geobacter extracellular cytochrome OmcS in Shewanella oneidensis Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-03-31 Tong Lin, Wenqi Ding, Danni Zhang, Zixuan You, Yun Yang, Feng Li, Dake Xu, Derek R. Lovley, Hao Song
The physiological role of Geobacter sulfurreducens extracellular cytochrome filaments is a matter of debate and the development of proposed electronic device applications of cytochrome filaments awaits methods for large‐scale cytochrome nanowire production. Functional studies in G. sulfurreducens are stymied by the broad diversity of redox‐active proteins on the outer cell surface and the redundancy
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Inhibition of hyphal formation together with biochar addition promotes erythritol production by Yarrowia lipolytica Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-03-29 Fangmei Liu, Kai Xia, Yuqing Chen, Ling Zhu, Lingzhi Zhu, Xuequn Zhao, Ruyi Sha, Jun Huang
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Sequence‐based design and construction of synthetic nanobody library Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-03-29 Chuanyong Liu, Yanping Li, Qinghua He, Jinheng Fu, Qingting Wei, Hao Lin, Ying Luo, Zhui Tu
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Development of inducible promoters for regulating gene expression in Clostridium tyrobutyricum for biobutanol production Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-03-28 Jun Feng, Qingke Wang, Zhen Qin, Xiaolong Guo, Hongxin Fu, Shang-Tian Yang, Jufang Wang
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Recent advances in biomedical applications of carbon and graphene quantum dots: A review Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-03-28 Abhijit Das, Manas Roy, Mitali Saha
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Prediction models for the flux decay profile and initial flux of microfiltration for therapeutic proteins Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-03-19 Kota Inoue, Yumiko Masuda, Tetsuo Torisu, Koichi Nonaka, Susumu Uchiyama
Microfiltration (MF) is an essential step during biopharmaceutical manufacturing. However, unexpected flux decay can occur. Although the flux decay profile and initial flux are important factors determining MF filterability, predicting them accurately is challenging, as the root cause of unexpected flux decay remains elusive. In this study, the methodology for developing a prediction model of flux
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Genome‐scale metabolic modeling of Thraustochytrium sp. RT2316‐16: Effects of nutrients on metabolism Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-03-19 Carolina Shene, Allison Leyton, Liset Flores, Daniela Chavez, Juan A. Asenjo, Yusuf Chisti
Marine thraustochytrids produce metabolically important lipids such as the long‐chain omega‐3 polyunsaturated fatty acids, carotenoids, and sterols. The growth and lipid production in thraustochytrids depends on the composition of the culture medium that often contains yeast extract as a source of amino acids. This work discusses the effects of individual amino acids provided in the culture medium
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An experimental and modeling approach to describe the deactivation of cellulases at the air–liquid interface Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-03-19 Laura Cachafeiro, Senta Heiss‐Blanquet, Damien Hudebine
Understanding the reaction mechanisms involved in the enzymatic hydrolysis of cellulose is important because it is kinetically the most limiting step of the bioethanol production process. The present work focuses on the enzymatic deactivation at the air–liquid interface, which is one of the aspects contributing to this global deactivation. This phenomenon has already been experimentally proven, but
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Itaconic acid production by co‐feeding of Ustilago maydis: A combined approach of experimental data, design of experiments, and metabolic modeling Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-03-18 Anita L. Ziegler, Lena Ullmann, Manuel Boßmann, Karla L. Stein, Ulf W. Liebal, Alexander Mitsos, Lars M. Blank
Itaconic acid is a platform chemical with a range of applications in polymer synthesis and is also discussed for biofuel production. While produced in industry from glucose or sucrose, co‐feeding of glucose and acetate was recently discussed to increase itaconic acid production by the smut fungus Ustilago maydis. In this study, we investigate the optimal co‐feeding conditions by interlocking experimental
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Residence time distribution in continuous virus filtration Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-03-18 Yu‐Cheng Chen, Gabriele Recanati, Fernando De Mathia, Dong‐Qiang Lin, Alois Jungbauer
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Improving stability of human three dimensional skin equivalents using plasma surface treatment Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-03-12 Suphanun Phuphanitcharoenkun, Fiona Louis, Yoshihiro Sowa, Michiya Matsusaki, Tanapat Palaga
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Transport‐controlled growth decoupling for self‐induced protein expression with a glycerol‐repressible genetic circuit Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-03-12 Alvaro R. Lara, Flavio Kunert, Vincent Vandenbroucke, Hilal Taymaz‐Nikerel, Luz María Martínez, Juan‐Carlos Sigala, Frank Delvigne, Guillermo Gosset, Jochen Büchs
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Continuous multi‐column capture of monoclonal antibodies with convective diffusive membrane adsorbers Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-03-12 Fabian Schmitz, Elias Knöchelmann, Thomas Kruse, Mirjana Minceva, Markus Kampmann
Downstream processing is the bottleneck in the continuous manufacturing of monoclonal antibodies (mAbs). To overcome throughput limitations, two different continuous processes with a novel convective diffusive protein A membrane adsorber (MA) were investigated: the rapid cycling parallel multi‐column chromatography (RC‐PMCC) process and the rapid cycling simulated moving bed (RC‐BioSMB) process. First
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Enhancing real‐time cell culture monitoring: Automated Raman model optimization with Taguchi method Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-03-08 Xiaoxiao Dong, Xu Yan, Yuxiang Wan, Dong Gao, Jingyu Jiao, Haibin Wang, Haibin Qu
Raman spectroscopy has found widespread usage in monitoring cell culture processes both in research and practical applications. However, commonly, preprocessing methods, spectral regions, and modeling parameters have been chosen based on experience or trial‐and‐error strategies. These choices can significantly impact the performance of the models. There is an urgent need for a simple, effective, and
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Bringing a scalable adaptive hybrid modeling framework closer to industrial use: Application on a multiscale fungal fermentation Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-03-07 Thomas Rydal, Jesper Frandsen, Gisela Nadal-Rey, Mads Orla Albæk, Pedram Ramin
Digitalization has paved the way for new paradigms such as digital shadows and digital twins for fermentation processes, opening the door for real-time process monitoring, control, and optimization. With a digital shadow, real-time model adaptation to accommodate complex metabolic phenomena such as metabolic shifts of a process can be monitored. Despite the many benefits of digitalization, the potential
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Host cell protein networks as a novel co-elution mechanism during protein A chromatography Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-03-07 Sherin Panikulam, Alexander Hanke, Frieder Kroener, Anette Karle, Oliver Anderka, Thomas K. Villiger, Nicolas Lebesgue
Host cell proteins (HCPs) are process-related impurities of therapeutic proteins produced in for example, Chinese hamster ovary (CHO) cells. Protein A affinity chromatography is the initial capture step to purify monoclonal antibodies or Fc-based proteins and is most effective for HCP removal. Previously proposed mechanisms that contribute to co-purification of HCPs with the therapeutic protein are
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ASIA: An automated stress‐inducible adaptor for enhanced stress protein expression in engineered Escherichia coli Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-03-07 Chuan‐Chieh Hsiang, I‐Son Ng
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Platform development for high‐throughput optimization of perfusion processes—Part II: Variation of perfusion rate strategies in microwell plates Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-03-04 Marie Dorn, Ciara Lucas, Kerensa Klottrup‐Rees, Ken Lee, Martina Micheletti
The biopharmaceutical industry is replacing fed‐batch with perfusion processes to take advantage of reduced capital and operational costs due to the operation at high cell densities (HCD) and improved productivities. HCDs are achieved by cell retention and continuous medium exchange, which is often based on the cell‐specific perfusion rate (CSPR). To obtain a cost‐productive process the perfusion rate
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Engineering an Escherichia coli based in vivo mRNA manufacturing platform Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-02-29 Edward Curry, George Muir, Jixin Qu, Zoltán Kis, Martyn Hulley, Adam Brown
Synthetic mRNA is currently produced in standardized in vitro transcription systems. However, this one‐size‐fits‐all approach has associated drawbacks in supply chain shortages, high reagent costs, complex product‐related impurity profiles, and limited design options for molecule‐specific optimization of product yield and quality. Herein, we describe for the first time development of an in vivo mRNA
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Correction to “Synergistic co-utilization of biomass-derived sugars enhances aromatic amino acid production by engineered Escherichia coli” Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-02-29
Liu, A., Machas, M., Mhatre, A., Hajinajaf, N., Sarnaik, A., Nichols, N., Frazer, S., Wang, X., Varman, A. M., Nielsen, D. R. (2024). Synergistic co-utilization of biomass-derived sugars enhances aromatic amino acid production by engineered Escherichia coli. Biotechnology and Bioengineering, 121:784–794. Throughout the “Results & Discussion” section, the text “Entner-Doudoroff pathway” and the abbreviated
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Simultaneous prediction of 16 quality attributes during protein A chromatography using machine learning based Raman spectroscopy models Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-02-28 Jiarui Wang, Jingyi Chen, Joey Studts, Gang Wang
Several key technologies for advancing biopharmaceutical manufacturing depend on the successful implementation of process analytical technologies that can monitor multiple product quality attributes in a continuous in-line setting. Raman spectroscopy is an emerging technology in the biopharma industry that promises to fit this strategic need, yet its application is not widespread due to limited success
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Quantitative characterization of tumor cell traction force on extracellular matrix by hydrogel microsphere stress sensor Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-02-26 Xingquan Ma, Cong Wang, Changchun Ji, Xiaoshan Cao, Yuqing Dong
Cell traction force (CTF) is a kind of active force that is a cell senses external environment and actively applies to the contact matrix which is currently a representative stress in cell–extracellular matrix (ECM) interaction. Studying the distribution and variation of CTF during cell–ECM interaction help to explain the impact of physical factors on cell behaviors from the perspective of mechanobiology
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Platform development for high‐throughput optimization of perfusion processes: Part I: Implementation of cell bleeds in microwell plates Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-02-23 Marie Dorn, Kerensa Klottrup‐Rees, Ken Lee, Martina Micheletti
The promise of continuous processing to increase yields and improve product quality of biopharmaceuticals while decreasing the manufacturing footprint is transformative. Developing and optimizing perfusion operations requires screening various parameters, which is expensive and time‐consuming when using benchtop bioreactors. Scale‐down models (SDMs) are the most feasible option for high‐throughput
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NIR spectroscopy—CNN‐enabled chemometrics for multianalyte monitoring in microbial fermentation Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-02-23 Shantanu Banerjee, Shyamapada Mandal, Naveen G. Jesubalan, Rijul Jain, Anurag S. Rathore
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Robust platform for inline Raman monitoring and control of perfusion cell culture Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-02-23 Boyong Wan, Misaal Patel, George Zhou, Michael Olma, Marco Bieri, Marvin Mueller, Emmanuel Appiah-Amponsah, Bhumit Patel, Karthik Jayapal
Perfusion cell culture has been gaining increasing popularity for biologics manufacturing due to benefits such as smaller footprint, increased productivity, consistent product quality and manufacturing flexibility, cost savings, and so forth. Process Analytics Technologies tools are highly desirable for effective monitoring and control of long-running perfusion processes. Raman has been widely investigated
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Enhanced sporulation of B. licheniformis BF-002 through automatic co-feeding of carbon and nitrogen sources Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-02-21 Jian Ding, Qingyuan Liu, Wenbiao Hou, Jun Cai, Bo Wang, Cheng Lu
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Biotechnology and Bioengineering: Volume 121, Number 3, March 2024 Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-02-19
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Bayesian cell therapy process optimization Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-02-19 Evan Claes, Tommy Heck, Kathleen Coddens, Maarten Sonnaert, Jan Schrooten, Jan Verwaeren
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A robust multinutrient kinetic model for enhanced lutein and biomass yields in mixotrophic microalgae cultivation: A step towards successful large-scale productions Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-02-19 Jihed Bentahar, Jean-Sébastien Deschênes
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Contributions of Chinese hamster ovary cell derived extracellular vesicles and other cellular materials to hollow fiber filter fouling during perfusion manufacturing of monoclonal antibodies Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-02-19 Yixiao Zhang, Sri Madabhushi, Tiffany Tang, Hassan Raza, David J. Busch, Xi Zhao, James Ormes, Sen Xu, Joseph Moroney, Rubin Jiang, Henry Lin, Ren Liu
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An optimized polymeric delivery system for piggyBac transposition Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-02-19 Daniel Nisakar Meenakshi Sundaram, Remant Bahadur K. C., Wei Fu, Hasan Uludağ
The piggyBac transposon/transposase system has been explored for long-term, stable gene expression to execute genomic integration of therapeutic genes, thus emerging as a strong alternative to viral transduction. Most studies with piggyBac transposition have employed physical methods for successful delivery of the necessary components of the piggyBac system into the cells. Very few studies have explored
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Supervised and unsupervised machine learning approaches for monitoring subvisible particles within an aluminum-salt adjuvanted vaccine formulation Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-02-19 David N. Greenblott, Caitlin V. Wood, Jingtao Zhang, Nelia Viza, Ramesh Chintala, Christopher P. Calderon, Theodore W. Randolph
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A low-temperature SPR-based assay for monoclonal antibody galactosylation and fucosylation assessment using FcγRIIA/B Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-02-14 Jimmy Gaudreault, Catherine Forest-Nault, Michel Gilbert, Yves Durocher, Olivier Henry, Gregory De Crescenzo
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Selective hydrophobic interaction chromatography for high purity of supercoiled DNA plasmids Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-02-14 Klemen Božič, Ajda Sedlar, Špela Kralj, Urh Černigoj, Aleš Štrancar, Rok Sekirnik
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New insights into Chlorella vulgaris applications Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-02-11 Mohammed Al-Hammadi, Mine Güngörmüşler
Environmental pollution is a big challenge that has been faced by humans in contemporary life. In this context, fossil fuel, cement production, and plastic waste pose a direct threat to the environment and biodiversity. One of the prominent solutions is the use of renewable sources, and different organisms to valorize wastes into green energy and bioplastics such as polylactic acid. Chlorella vulgaris
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Deep hybrid modeling of a HEK293 process: Combining long short-term memory networks with first principles equations Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-02-11 João R. C. Ramos, José Pinto, Gil Poiares-Oliveira, Ludovic Peeters, Patrick Dumas, Rui Oliveira
The combination of physical equations with deep learning is becoming a promising methodology for bioprocess digitalization. In this paper, we investigate for the first time the combination of long short-term memory (LSTM) networks with first principles equations in a hybrid workflow to describe human embryonic kidney 293 (HEK293) culture dynamics. Experimental data of 27 extracellular state variables
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Accelerating attribute-focused process and product development through the development and deployment of autonomous process analytical technology platform system Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-02-08 Chao-Hsiang (Richard) Wu, Becky Chan, Zac Sarich, Yaokai Duan, Janice Chen, Jiu-Li Song, Mike Berke, Les P. Miranda, Chetan T. Goudar
Enabling real-time monitoring and control of the biomanufacturing processes through product quality insights continues to be an area of focus in the biopharmaceutical industry. The goal is to manufacture products with the desired quality attributes. To realize this rigorous attribute-focused Quality by Design approach, it is critical to support the development of processes that consistently deliver
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Nanopore sequencing improves construction of customized CRISPR-based gene activation libraries Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-01-31 Handing Wang, Heng Yih Tan, Jiazhang Lian, Kang Zhou
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Cell culture product quality attribute prediction using convolutional neural networks and Raman spectroscopy Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-01-29 Hamid Khodabandehlou, Mohammad Rashedi, Tony Wang, Aditya Tulsyan, Gregg Schorner, Christopher Garvin, Cenk Undey
Advanced process control in the biopharmaceutical industry often lacks real-time measurements due to resource constraints. Raman spectroscopy and Partial Least Squares (PLS) models are often used to monitor bioprocess cultures in real-time. In spite of the ease of training, the accuracy of the PLS model is impacted if it is not used to predict quality attributes for the cell lines it is trained on
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A novel experimental method to determine substrate uptake kinetics of gaseous substrates applied to the carbon monoxide-fermenting Clostridium autoethanogenum Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-01-24 Maximilienne T. Allaart, Charilaos Korkontzelos, Diana Z. Sousa, Robbert Kleerebezem
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Computer-directed rational design enhanced the thermostability of carbonyl reductase LsCR for the synthesis of ticagrelor precursor Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-01-24 Shen-Yuan Xu, Rong-Liang Chu, Hua-Tao Liu, Chun-Yue Weng, Ya-Jun Wang, Yu-Guo Zheng
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Giving the cells what they need when they need it: Biosensor-based feeding control Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-01-23 Romain Kinet, Anne Richelle, Michael Colle, Didier Demaegd, Moritz von Stosch, Matthew Sanders, Hannah Sehrt, Frank Delvigne, Philippe Goffin
“Giving the cells exactly what they need, when they need it” is the core idea behind the proposed bioprocess control strategy: operating bioprocess based on the physiological behavior of the microbial population rather than exclusive monitoring of environmental parameters. We are envisioning to achieve this through the use of genetically encoded biosensors combined with online flow cytometry (FCM)
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Genetic algorithm-based semisupervised convolutional neural network for real-time monitoring of Escherichia coli fermentation of recombinant protein production using a Raman sensor Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-01-22 Yuan Liu, Xiaotian Zhou, Teng Wang, An Luo, Zhaojun Jia, Xingquan Pan, Weiqi Cai, Mengge Sun, Xuezhong Wang, Zhenguo Wen, Guangzheng Zhou
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Factors affecting product association as a mechanism of host-cell protein persistence in bioprocessing Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-01-19 Young Hoon Oh, Matthew L. Becker, Kerri M. Mendola, Leila H. Choe, Lie Min, Kelvin H. Lee, Yinges Yigzaw, Alexander Seay, Jerome Bill, Xuanwen Li, David J. Roush, Steven M. Cramer, Stefano Menegatti, Abraham M. Lenhoff
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Engineering of a decellularized bovine skin coated with antibiotics-loaded electrospun fibers with synergistic antibacterial activity for the treatment of infectious wounds Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-01-17 Sanaz Alizadeh, Jila Majidi, Mozhgan Jahani, Zahra Esmaeili, Zeinab Nokhbedehghan, Zahra Aliakbar Ahovan, Hajar Nasiri, Amir Mellati, Ali Hashemi, Narendra Pal Singh Chauhan, Mazaher Gholipourmalekabadi
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Realization of digital twin for dynamic control toward sample variation of ion exchange chromatography in antibody separation Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-01-17 Ce Shi, Xu-Jun Chen, Xue-Zhao Zhong, Yan Yang, Dong-Qiang Lin, Ran Chen
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Cover Image, Volume 121, Number 2, February 2024 Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-01-14 Oliver McRae, Peter L. L. Walls, Venkatesh Natarajan, Chris Antoniou, James C. Bird
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Acoustic levitation as a tool for cell-driven self-organization of human cell spheroids during long-term 3D culture Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-01-15 Lucile Rabiet, Lousineh Arakelian, Nathan Jeger-Madiot, Duván Rojas García, Jérôme Larghero, Jean-Luc Aider
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Contactless face video based vital signs detection framework for continuous health monitoring using feature optimization and hybrid neural network Biotechnol. Bioeng. (IF 3.8) Pub Date : 2024-01-14 Anju Anil Jalaja, Maruthai Kavitha
Continuous monitoring of vital signs such as respiration and heart rate is essential to detect and predict conditions that may affect the patient's well-being. To detect these vital signs most medical systems use contact sensors. They are not feasible for long term monitoring and are not repeatable. Vital signs using facial video-noncontact monitoring are becoming increasingly important. Researchers