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The LCG1-OsBP5/OsEBP89-Wx module regulates the grain chalkiness and taste quality in rice Plant Biotech. J. (IF 10.1) Pub Date : 2024-09-23 Bin Tu, Tao Zhang, Pin Liu, Wen Yang, Ling Zheng, Ying Dai, Hao Wang, Song Lin, Zehua Zhang, Xiaohang Zheng, Mengting Yuan, Yong Chen, Xiaobo Zhu, Hua Yuan, Ting Li, Jiawei Xiong, Zhaohui Zhong, Weilan Chen, Bingtian Ma, Peng Qin, Yuping Wang, Shigui Li
It is well known that the overall quality of japonica/geng rice is superior to that of indica/xian rice varieties. However, the molecular mechanisms underlying the quality disparities between these two subspecies of rice are still largely unknown. In this study, we have pinpointed a gene homologous to SLR1, termed LCG1, exhibiting significant expression during early caryopsis development and playing
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A gap-free genome of pillar peach (Prunus persica L.) provides new insights into branch angle and double flower traits Plant Biotech. J. (IF 10.1) Pub Date : 2024-09-22 Haipeng Zhang, Xiaodong Lian, Fan Gao, Conghao Song, Beibei Feng, Xianbo Zheng, Xiaobei Wang, Nan Hou, Jun Cheng, Wei Wang, Langlang Zhang, Jidong Li, Xia Ye, Jiancan Feng, Bin Tan
Peach (Prunus persica L.), a deciduous fruit tree in the Rosaceae family, is widely cultivated around the world. The release of the peach genome which significantly propelled the progress of gene mapping (Lian et al., 2022; Verde et al., 2013; Zhou et al., 2023). However, the existing peach genomes all contain multiple gaps (Table S1), which may lead to inaccurate gene annotation or gene mapping (Zhou
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Knockout of phosphatidate phosphohydrolase genes confers broad-spectrum disease resistance in plants Plant Biotech. J. (IF 10.1) Pub Date : 2024-09-22 Qiuwen Gong, Gan Sha, Xinyu Han, Zhenhua Guo, Lei Yang, Ting Chen, Wei Yang, Ronglei Tan, Meng Liu, Fengdie Xia, Guang Chen, Yufei Li, Xin Shen, Kabin Xie, Guangqin Cai, Honghong Hu, Jie Luo, Qiang Li, Guotian Li
Phosphatidic acid (PA) is considered a second messenger that interacts with protein kinases, phosphatases and NADPH oxidases (Kong et al., 2024), amplifying the signal to initiate plant defence responses (Li and Wang, 2019). In rice, mutation of RBL1 causes the accumulation of PA, enhancing multipathogen resistance (Sha et al., 2023). In our previous study, we attempted to rescue rbl1 mutant by overexpressing
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Identifying resistant mutations in the herbicide target site of the plant 4-hydroxyphenylpyruvate dioxygenase Plant Biotech. J. (IF 10.1) Pub Date : 2024-09-20 Mugui Wang, Yingli Zhong, Yuxin He, Jiyong Xie, Hongtao Xie, Yingying Wang, Li Xue, Xin Wang, Gaurav Zinta, Vipasha Verma, Hongzhi Wang, Yanfei Mao, Jian-Kang Zhu
Weed species have increasingly emerged with resistance against previously effective herbicides, such as glyphosate and inhibitors of acetyl coenzyme A carboxylase (ACCase) and acetolactate synthase (ALS) (Heap, 2024). Owing to its novel mode of action, 4-hydroxyphenylpyruvate dioxygenase (HPPD)-inhibitors are effective in controlling herbicide-resistant weeds and recently attracted much attention.
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KRN5b regulates maize kernel row number through mediating phosphoinositol signalling Plant Biotech. J. (IF 10.1) Pub Date : 2024-09-20 Xiaomeng Shen, Lei Liu, Thu Tran, Qiang Ning, Manfei Li, Liangliang Huang, Ran Zhao, Yunfu Li, Xiangyu Qing, David Jackson, Yan Bai, Weibin Song, Jinsheng Lai, Zuxin Zhang, Haiming Zhao
Kernel row number (KRN) is a major yield related trait for maize (Zea mays L.) and is also a major goal of breeders, as it can increase the number of kernels per plant. Thus, identifying new genetic factors involving in KRN formation may accelerate improving yield-related traits genetically. We herein describe a new kernel number-related gene (KRN5b) identified from KRN QTL qKRN5b and encoding an inositol
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A telomere‐to‐telomere haplotype‐resolved genome of white‐fruited strawberry reveals the complexity of fruit colour formation of cultivated strawberry Plant Biotech. J. (IF 10.1) Pub Date : 2024-09-20 Junxiang Zhang, Shuang Liu, Shuo Zhao, Yuxin Nie, Zhihong Zhang
Cultivated strawberry (Fragaria × ananassa, 2n = 8x = 56) is an important horticultural crop with substantial economic and nutritional value. The improvement of cultivated strawberry is more challenging not only in its octoploid genome but also in the frequent homoeologous exchanges and polyploidization, which replaces substantial portions of some subgenomes with sequences derived from ancestrally
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Genomes of Aegilops umbellulata provide new insights into unique structural variations and genetic diversity in the U‐genome for wheat improvement Plant Biotech. J. (IF 10.1) Pub Date : 2024-09-18 Jatinder Singh, Santosh Gudi, Peter J. Maughan, Zhaohui Liu, James Kolmer, Meinan Wang, Xianming Chen, Matthew N. Rouse, Pauline Lasserre‐Zuber, Héléne Rimbert, Sunish Sehgal, Jason D. Fiedler, Frédéric Choulet, Maricelis Acevedo, Rajeev Gupta, Upinder Gill
SummaryAegilops umbellulata serve as an important reservoir for novel biotic and abiotic stress tolerance for wheat improvement. However, chromosomal rearrangements and evolutionary trajectory of this species remain to be elucidated. Here, we present a comprehensive investigation into Ae. umbellulata genome by generating a high‐quality near telomere‐to‐telomere genome assembly of PI 554389 and resequencing
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miR827 orchestrates the regulation of SPX‐MFS1 and SPX‐MFS5 with the assistance of lncRNA767 to enhance phosphate starvation tolerance and maize development Plant Biotech. J. (IF 10.1) Pub Date : 2024-09-17 Lei Chen, Juan He, Xufeng Wang, Shiru Zhang, Jinkang Pan, Jianxiang Peng, Beixin Mo, Lin Liu
SummaryMicroRNA827 (miR827) is functionally conserved among different plant species and displays species‐specific characteristics, but the mechanisms by which miR827 regulates phosphate (Pi) starvation tolerance and maize development remain elusive. We found that miR827 selectively targets the Pi transporter genes SPX‐MFS1 and SPX‐MFS5. miR827 overexpression improved the Pi starvation tolerance, plant
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High-quality haplotype-resolved chromosome assembly provides evolutionary insights and targeted steviol glycosides (SGs) biosynthesis in Stevia rebaudiana Bertoni Plant Biotech. J. (IF 10.1) Pub Date : 2024-09-16 Mamta Masand, Shikha Sharma, Sangeeta Kumari, Poonam Pal, Aasim Majeed, Gopal Singh, Ram Kumar Sharma
Stevia rebaudiana Bertoni is popular source of plant-derived low/no-calorie natural sweeteners (LNCSs), collectively known as steviol glycosides (SGs). Nevertheless, genetic predisposition for targeted biosynthesis of SGs is complex due to multi-substrate functionality of key uridine diphosphate glycosyltransferases (UGTs). Here, we created a high-quality monoploid assembly of 1.34 Gb with N50 value
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Metabolic engineering of vitamin D3 in Solanaceae plants Plant Biotech. J. (IF 10.1) Pub Date : 2024-09-16 Marianna Boccia, Kerstin Ploβ, Maritta Kunert, Radhika Keshan, Mustafa Hatam, Veit Grabe, Sarah E. O'Connor, Prashant D. Sonawane
Vitamin D is a lipid-soluble sterol that plays an essential role in human health. Deficiency of this vitamin increases the risk of osteoporosis, hypertension, autoimmune diseases, infectious disease, diabetes and cancer. Vitamin D exists in two major forms: vitamin D3 (cholecalciferol), mainly found in animal food source, and vitamin D2 (ergocalciferol), typically present in sundried and ultraviolet-B
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Natural variations in the Cis-elements of GhRPRS1 contributing to petal colour diversity in cotton Plant Biotech. J. (IF 10.1) Pub Date : 2024-09-16 Wei Hu, Yanli Chen, Zhenzhen Xu, Linqiang Liu, Da Yan, Miaoyang Liu, Qingdi Yan, Yihao Zhang, Lan Yang, Chenxu Gao, Renju Liu, Wenqiang Qin, Pengfei Miao, Meng Ma, Peng Wang, Babai Gao, Fuguang Li, Zhaoen Yang
The cotton genus comprises both diploid and allotetraploid species, and the diversity in petal colour within this genus offers valuable targets for studying orthologous gene function differentiation and evolution. However, the genetic basis for this diversity in petal colour remains largely unknown. The red petal colour primarily comes from C, G, K, and D genome species, and it is likely that the common
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A gain‐of‐function mutation at the C‐terminus of FT‐D1 promotes heading by interacting with 14‐3‐3A and FDL6 in wheat Plant Biotech. J. (IF 10.1) Pub Date : 2024-09-14 Yuting Li, Hongchun Xiong, Huijun Guo, Yongdun Xie, Linshu Zhao, Jiayu Gu, Huiyuan Li, Shirong Zhao, Yuping Ding, Chunyun Zhou, Zhengwu Fang, Luxiang Liu
SummaryVernalization and photoperiod pathways converging at FT1 control the transition to flowering in wheat. Here, we identified a gain‐of‐function mutation in FT‐D1 that results in earlier heading date (HD), and shorter plant height and spike length in the gamma ray‐induced eh1 wheat mutant. Knockout of the wild‐type and overexpression of the mutated FT‐D1 indicate that both alleles are functional
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A natural variation contributes to sugar accumulation in fruit during tomato domestication Plant Biotech. J. (IF 10.1) Pub Date : 2024-09-13 Zhiqiang Wang, Yarong Zhao, Minmin Zheng, Shuojun Yu, Yang Gao, Guangtao Zhu, Jian‐Kang Zhu, Kai Hua, Zhen Wang
Tomato is the most widely consumed fruit and vegetable crop in the world, serving as an important source of micronutrients in human diet (Zhu et al., 2018). The impact of sugar on the taste of tomato fruits is generally estimated by determining their total soluble solids (TSS) accumulation (Kader, 2008), therefore, the determination of TSS is responsible for the fruit quality of tomato designed for
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Editing of OsPsaL gene improves both yield and antiviral immunity in rice Plant Biotech. J. (IF 10.1) Pub Date : 2024-09-13 Ruifang Zhang, Hehong Zhang, Lulu Li, Yanjun Li, Kaili Xie, Jianping Chen, Zongtao Sun
Rice (Oryza sativa) is a staple food supply for over half of the global population. Various phytopathogens including viruses pose a significant threat to rice yield and quality. Southern rice black-streaked dwarf virus (SRBSDV), belonged to the genus Fijivirus, family Reoviridae, has become a major virus species leading to substantial crop losses in Asian nations (Zhang et al., 2023). Traditional breeding
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Comparative evaluation of gene copy number estimation techniques in genetically modified crops: insights from Southern blotting, qPCR, dPCR and NGS Plant Biotech. J. (IF 10.1) Pub Date : 2024-09-12 Wenting Xu, Jingang Liang, Fan Wang, Litao Yang
Gene copy number is crucial for understanding genomic architecture and its implications in plant and animal genetics (Alonge et al., 2020; Castagnone-Sereno et al., 2019). In agriculture, variations in gene copy number (CNVs) are vital as they affect yield, stress resistance and metabolic capabilities (Yuan et al., 2021). Transgenesis, involving the introduction of foreign DNA into plant genomes, has
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High‐yield, plant‐based production of an antimicrobial peptide with potent activity in a mouse model Plant Biotech. J. (IF 10.1) Pub Date : 2024-09-12 Shahid Chaudhary, Zahir Ali, Aarón Pantoja‐Angles, Sherin Abdelrahman, Cynthia Olivia Baldelamar Juárez, Gundra Sivakrishna Rao, Pei‐Ying Hong, Charlotte Hauser, Magdy Mahfouz
SummaryPlants offer a promising chassis for the large‐scale, cost‐effective production of diverse therapeutics, including antimicrobial peptides (AMPs). However, key advances will reduce production costs, including simplifying the downstream processing and purification steps. Here, using Nicotiana benthamiana plants, we present an improved modular design that enables AMPs to be secreted via the endomembrane
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Towards chloroplastic nanofactories: formation of proteinaceous scaffolds for metabolic engineering Plant Biotech. J. (IF 10.1) Pub Date : 2024-09-06 Matthew E. Dwyer, John E. Froehlich, Daniel A. Raba, Melissa Borrusch, Linda Danhof, Naveen Sharma, Eric J. Young, Federica Brandizzi, Christoph Benning, Cheryl A. Kerfeld
The evolution of eukaryotic lipid-bound organelles allows for specialized metabolism to occur within spatially distinct metabolic landscapes within the same cell. However, this strategy of compartmentalization is not unique to eukaryotic organisms. Many bacteria, spread across 45 different phyla, contain loci that encode for specialized bacterial microcompartments (BMCs) (Sutter et al., 2021). The
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eIF2Bβ confers resistance to Turnip mosaic virus by recruiting ALKBH9B to modify viral RNA methylation Plant Biotech. J. (IF 10.1) Pub Date : 2024-09-04 Tongyun Sha, Zhangping Li, Shirui Xu, Tongbing Su, Jannat Shopan, Xingming Jin, Yueying Deng, Xiaolong Lyu, Zhongyuan Hu, Mingfang Zhang, Jinghua Yang
SummaryEukaryotic translation initiation factors (eIFs) are the primary targets for overcoming RNA virus resistance in plants. In a previous study, we mapped a BjeIF2Bβ from Brassica juncea representing a new class of plant virus resistance genes associated with resistance to Turnip mosaic virus (TuMV). However, the mechanism underlying eIF2Bβ‐mediated virus resistance remains unclear. In this study
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ScDB: A comprehensive database dedicated to Saccharum, facilitating functional genomics and molecular biology studies in sugarcane Plant Biotech. J. (IF 10.1) Pub Date : 2024-08-30 Siyuan Chen, Xiaoxi Feng, Zhe Zhang, Xiuting Hua, Qing Zhang, Chengjie Chen, Jiawei Li, Xiaojing Liu, Chenyu Weng, Baoshan Chen, Muqing Zhang, Wei Yao, Haibao Tang, Ray Ming, Jisen Zhang
Sugarcane is the world's important sugar crop, serving as the primary feedstock for the production of sugar and biofuels. Modern sugarcane cultivar resulting from deliberate interspecific hybridization between Saccharum officinarum and Saccharum spontaneum. The utilization of wild resources is essential for the development of high-quality sugarcane varieties, and the genomic and omics analyses of these
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AaMYC3 bridges the regulation of glandular trichome density and artemisinin biosynthesis in Artemisia annua Plant Biotech. J. (IF 10.1) Pub Date : 2024-08-27 Mingyuan Yuan, Yinguo Sheng, Jingjing Bao, Wenkai Wu, Guibin Nie, Lingjian Wang, Junfeng Cao
SummaryArtemisinin, the well‐known natural product for treating malaria, is biosynthesised and stored in the glandular‐secreting trichomes (GSTs) of Artemisia annua. While numerous efforts have clarified artemisinin metabolism and regulation, the molecular association between artemisinin biosynthesis and GST development remains elusive. Here, we identified AaMYC3, a bHLH transcription factor of A.
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Natural variation in the Tn1a promoter regulates tillering in rice Plant Biotech. J. (IF 10.1) Pub Date : 2024-08-27 Tao Yang, Xiaoqian Ma, Quan Zhang, Lin Li, Rui Zhu, An Zeng, Wanying Liu, Haixia Liu, Yulong Wang, Shichen Han, Najeeb Ullah Khan, Jinjie Li, Zichao Li, Zhanying Zhang, Hongliang Zhang
SummaryRice tillering is an important agronomic trait that influences plant architecture and ultimately affects yield. This can be genetically improved by mining favourable variations in genes associated with tillering. Based on a previous study on dynamic tiller number, we cloned the gene Tiller number 1a (Tn1a), which encodes a membrane‐localised protein containing the C2 domain that negatively regulates
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A resource for functional investigation of miRNAs in rice responses to viral infection Plant Biotech. J. (IF 10.1) Pub Date : 2024-08-24 Baogang Zhang, Xiong Zhang, Wenji Li, Dezhuo Pan, Baining Ma, Xinhui Duan, Chaoyi Dong, Lu Wang, Mingfu Zhao, Shanshan Zhao, Shuai Zhang, Jianguo Wu
In rice agriculture, the rice grassy stunt virus (RGSV) and rice ragged stunt virus (RRSV) present significant biosafety challenges. RGSV, a Bunyaviridae family virus with single-stranded RNA and RRSV, a double-stranded RNA virus from the Reoviridae family, are mainly transmitted by the brown planthopper, posing a co-infection risk that can cause rice yellowing syndrome. This syndrome can severely
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SUBSTANDARD STARCH GRAIN7 regulates starch grain size and endosperm development in rice Plant Biotech. J. (IF 10.1) Pub Date : 2024-08-24 Haigang Yan, Yulong Ren, Binglei Zhang, Jie Jin, Feilong Du, Zhuangzhuang Shan, Yushuang Fu, Yun Zhu, Xin Wang, Changyuan Zhu, Yue Cai, Jie Zhang, Fan Wang, Xiao Zhang, Rongqi Wang, Yongxiang Wang, Hancong Xu, Ling Jiang, Xi Liu, Shanshan Zhu, Qibing Lin, Cailin Lei, Zhijun Cheng, Yihua Wang, Wenwei Zhang, Jianmin Wan
SummaryStarch is synthesized as insoluble, semicrystalline particles within plant chloroplast and amyloplast, which are referred to as starch grains (SGs). The size and morphology of SGs in the cereal endosperm are diverse and species–specific, representing a key determinant of the suitability of starch for industrial applications. However, the molecular mechanisms modulating SG size in cereal endosperm
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MdWRKY31‐MdNAC7 regulatory network: orchestrating fruit softening by modulating cell wall‐modifying enzyme MdXTH2 in response to ethylene signalling Plant Biotech. J. (IF 10.1) Pub Date : 2024-08-24 Jia‐Hui Wang, Quan Sun, Chang‐Ning Ma, Meng‐Meng Wei, Chu‐Kun Wang, Yu‐Wen Zhao, Wen‐Yan Wang, Da‐Gang Hu
SummarySoftening in fruit adversely impacts their edible quality and commercial value, leading to substantial economic losses during fruit ripening, long‐term storage, long‐distance transportation, and marketing. As the apple fruit demonstrates climacteric respiration, its firmness decreases with increasing ethylene release rate during fruit ripening and postharvest storage. However, the molecular
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Multiplex Expression Cassette Assembly: A flexible and versatile method for building complex genetic circuits in conventional vectors Plant Biotech. J. (IF 10.1) Pub Date : 2024-08-23 Xun Jiang, Zhuoxiang Zhang, Xiuming Wu, Changmei Li, Xuan Sun, Yiting Li, Aixia Chang, Aiguo Yang, Changqing Yang
SummaryThe manipulation of multiple transcription units for simultaneous and coordinated expression is not only key to building complex genetic circuits to accomplish diverse functions in synthetic biology, but is also important in crop breeding for significantly improved productivity and overall performance. However, building constructs with multiple independent transcription units for fine‐tuned
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Editing and genome‐wide analysis upstream open reading frames contributes to enhancing salt tolerance in tomato Plant Biotech. J. (IF 10.1) Pub Date : 2024-08-22 Chunping Jia, Juan Wang, Bin Guo, Tao Yang, Haitao Yang, Baike Wang, Qinghui Yu
SummaryThe salinization of soil constitutes a substantial hindrance to the advancement of sustainable agriculture. Our research seeks to elucidate the role of a Rab GTPase‐activating protein (RabGAP) family member, SlRabGAP22, in salt tolerance and its translational regulation under salt stress in tomatoes, employing gene‐editing techniques and ribosome profiling methodologies. Findings demonstrate
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DEAD‐box RNA helicase RH20 positively regulates RNAi‐based antiviral immunity in plants by associating with SGS3/RDR6 bodies Plant Biotech. J. (IF 10.1) Pub Date : 2024-08-21 Zhiyan Wen, Rujian Hu, Qinglin Pi, Dingliang Zhang, Jiangning Duan, Zhen Li, Qian Li, Xiaoyun Zhao, Meng Yang, Xiaofei Zhao, Deshui Liu, Zhen Su, Dawei Li, Yongliang Zhang
SummaryRNA silencing plays a crucial role in defending against viral infections in diverse eukaryotic hosts. Despite extensive studies on core components of the antiviral RNAi pathway such as DCLs, AGOs and RDRs proteins, host factors involved in antiviral RNAi remain incompletely understood. In this study, we employed the proximity labelling approach to identify the host factors required for antiviral
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Ustilaginoidea virens secreted effector UvSec117 hijacks OsWRKY31‐OsAOC module to suppress jasmonic acid‐mediated immunity in rice Plant Biotech. J. (IF 10.1) Pub Date : 2024-08-16 Yuhang Duan, Guogen Yang, Jintian Tang, Yuan Fang, Hailin Wang, Zhaoyun Wang, Hao Liu, Xiaolin Chen, Junbin Huang, Jing Chen, Qiutao Xu, Lu Zheng, Xiaoyang Chen
Rice false smut (RFS) caused by Ustilaginoidea virens is one of the most important disease in rice (Oryza sativa)-growing regions worldwide. RFS not only causes rice yield losses but also potentially threatens human and animal health by producing cyclopeptide mycotoxins (Sun et al., 2020). Introducing genetically encoded resistance is an environmentally friendly, economical approach to controlling
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Wild rice: unlocking the future of rice breeding Plant Biotech. J. (IF 10.1) Pub Date : 2024-08-16 Xiaoming Zheng, Youlin Peng, Jiyue Qiao, Robert Henry, Qian Qian
SummaryGermplasm resources serve as the foundations of advancements in breeding and are crucial for maintaining food security. Wild rice species of the genus Oryza include rich sources of genetic diversity and high adaptability, making them a substantial resource for rice breeding. The discovery of wild‐type cytoplasmic male sterility resources enabled the achievement of the ‘three lines’ goal in hybrid
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Loss‐of‐function of LIGULELESS1 activates the jasmonate pathway and promotes maize resistance to corn leaf aphids Plant Biotech. J. (IF 10.1) Pub Date : 2024-08-15 Shijie Huang, Chuanhong Wang, Ling Wang, Shuai Li, Tengyue Wang, Zhen Tao, Yibing Zhao, Jing Ma, Mengjie Zhao, Xinqiao Zhang, Lei Wang, Chuanxiao Xie, Peijin Li
SummaryCorn leaf aphids (Rhopalosiphum maidis) are highly destructive pests of maize (Zea mays) that threaten growth and seed yield, but resources for aphid resistance are scarce. Here, we identified an aphid‐resistant maize mutant, resistance to aphids 1 (rta1), which is allelic to LIGULELESS1 (LG1). We confirmed LG1's role in aphid resistance using the independent allele lg1‐2, allelism tests and
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Pan‐genome analysis of 13 Spinacia accessions reveals structural variations associated with sex chromosome evolution and domestication traits in spinach Plant Biotech. J. (IF 10.1) Pub Date : 2024-08-03 Hongbing She, Zhiyuan Liu, Zhaosheng Xu, Helong Zhang, Jian Wu, Feng Cheng, Xiaowu Wang, Wei Qian
SummaryStructural variations (SVs) are major genetic variants that can be involved in the origin, adaptation and domestication of species. However, the identification and characterization of SVs in Spinacia species are rare due to the lack of a pan‐genome. Here, we report eight chromosome‐scale assemblies of cultivated spinach and its two wild species. After integration with five existing assemblies
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Conserved features and diversity attributes of chimeric RNAs across accessions in four plants Plant Biotech. J. (IF 10.1) Pub Date : 2024-08-01 Jia Cong, Sinan Zhang, Qi Zhang, Xiting Yu, Jiazhi Huang, Xin Wei, Xuehui Huang, Jie Qiu, Xiaoyi Zhou
SummaryAs a non‐collinear expression form of genetic information, chimeric RNAs increase the complexity of transcriptome in diverse organisms. Although chimeric RNAs have been identified in plants, few common features have been revealed. Here, we systemically explored the landscape of chimeric RNAs across multi‐accession and multi‐tissue using pan‐genome and transcriptome data of four plants: rice
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GhSBI1, a CUP‐SHAPED COTYLEDON 2 homologue, modulates branch internode elongation in cotton Plant Biotech. J. (IF 10.1) Pub Date : 2024-07-26 Weiping Zhong, Lanxin Wu, Yan Li, Xiaxuan Li, Junyi Wang, Jingwen Pan, Shouhong Zhu, Shentao Fang, Jinbo Yao, Yongshan Zhang, Wei Chen
SummaryBranch length is an important plant architecture trait in cotton (Gossypium) breeding. Development of cultivars with short branch has been proposed as a main object to enhance cotton yield potential, because they are suitable for high planting density. Here, we report the molecular cloning and characterization of a semi‐dominant quantitative trait locus, Short Branch Internode 1(GhSBI1), which
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Optimized dCas9 programmable transcriptional activators for plants Plant Biotech. J. (IF 10.1) Pub Date : 2024-07-26 Matthew H. Zinselmeier, Juan Armando Casas‐Mollano, Jonathan Cors, Adam Sychla, Stephen C. Heinsch, Daniel F. Voytas, Michael J. Smanski
Understanding how gene expression impacts plant development and physiology is important for crop engineering. Programmable transcriptional activators (PTAs), including CRISPR-Cas activators, have relied on a limited number of transcriptional activation domains (ADs) (Casas-Mollano et al., 2020; Lowder et al., 2018; Pan et al., 2021; Papikian et al., 2019). Usually, the VP64 domain, derived from herpes
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Unravelling the genetic basis and regulation networks related to fibre quality improvement using chromosome segment substitution lines in cotton Plant Biotech. J. (IF 10.1) Pub Date : 2024-07-24 Guoan Qi, Zhanfeng Si, Lisha Xuan, Zegang Han, Yan Hu, Lei Fang, Fan Dai, Tianzhen Zhang
SummaryThe elucidation of genetic architecture and molecular regulatory networks underlying complex traits remains a significant challenge in life science, largely due to the substantial background effects that arise from epistasis and gene–environment interactions. The chromosome segment substitution line (CSSL) is an ideal material for genetic and molecular dissection of complex traits due to its
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ZmPHR1 contributes to drought resistance by modulating phosphate homeostasis in maize Plant Biotech. J. (IF 10.1) Pub Date : 2024-07-22 Meng‐Zhi Tian, Hai‐Feng Wang, Yan Tian, Jie Hao, Hui‐Ling Guo, Li‐Mei Chen, Ya‐Kang Wei, Shi‐Hao Zhan, Hong‐Tao Yu, Yi‐Fang Chen
SummaryAs an essential macronutrient, phosphorus (P) is often a limiting nutrient because of its low availability and mobility in soils. Drought is a major environmental stress that reduces crop yield. How plants balance and combine P‐starvation responses (PSRs) and drought resistance is unclear. In this study, we identified the transcription factor ZmPHR1 as a major regulator of PSRs that modulates
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PhieDBEs: a DBD‐containing, PAM‐flexible, high‐efficiency dual base editor toolbox with wide targeting scope for use in plants Plant Biotech. J. (IF 10.1) Pub Date : 2024-07-19 Zhiye Zheng, Taoli Liu, Nan Chai, Dongchang Zeng, Ruixiang Zhang, Yang Wu, Jiaxuan Hang, Yuxin Liu, Qindi Deng, Jiantao Tan, Jialin Liu, Xianrong Xie, Yao‐Guang Liu, Qinlong Zhu
SummaryDual base editors (DBEs) enable simultaneous A‐to‐G and C‐to‐T conversions, expanding mutation types. However, low editing efficiency and narrow targeting range limit the widespread use of DBEs in plants. The single‐strand DNA binding domain of RAD51 DBD can be fused to base editors to improve their editing efficiency. However, it remains unclear how the DBD affects dual base editing performance
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Polyamines: pleiotropic molecules regulating plant development and enhancing crop yield and quality Plant Biotech. J. (IF 10.1) Pub Date : 2024-07-18 Haishan Yang, Yinyin Fang, Zhiman Liang, Tian Qin, Ji‐Hong Liu, Taibo Liu
SummaryPolyamines (PAs) are pleiotropic bioorganic molecules. Cellular PA contents are determined by a balance between PA synthesis and degradation. PAs have been extensively demonstrated to play vital roles in the modulation of plant developmental processes and adaptation to various environmental stresses. In this review, the latest advances on the diverse roles of PAs in a range of developmental
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Genetic variation in the aquaporin TONOPLAST INTRINSIC PROTEIN 4;3 modulates maize cold tolerance Plant Biotech. J. (IF 10.1) Pub Date : 2024-07-18 Rong Zeng, Xiaoyan Zhang, Guangshu Song, Qingxue Lv, Minze Li, Diyi Fu, Zhuo Zhang, Lei Gao, Shuaisong Zhang, Xiaohong Yang, Feng Tian, Shuhua Yang, Yiting Shi
SummaryCold stress is a major abiotic stress that threatens maize (Zea mays L.) production worldwide. Understanding the molecular mechanisms underlying cold tolerance is crucial for breeding resilient maize varieties. Tonoplast intrinsic proteins (TIPs) are a subfamily of aquaporins in plants. Here, we report that TIP family proteins are involved in maize cold tolerance. The expression of most TIP
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Enhanced efficacy of glycoengineered rice cell‐produced trastuzumab Plant Biotech. J. (IF 10.1) Pub Date : 2024-07-17 Jun‐Hye Shin, Sera Oh, Mi‐Hwa Jang, Seok‐Yong Lee, Chanhong Min, Young‐Jae Eu, Hilal Begum, Jong‐Chan Kim, Gap Ryol Lee, Han‐Bin Oh, Matthew J. Paul, Julian K.‐C. Ma, Ho‐Shin Gwak, Hyewon Youn, Seong‐Ryong Kim
SummaryFor several decades, a plant‐based expression system has been proposed as an alternative platform for the production of biopharmaceuticals including therapeutic monoclonal antibodies (mAbs), but the immunogenicity concerns associated with plant‐specific N‐glycans attached in plant‐based biopharmaceuticals has not been completely solved. To eliminate all plant‐specific N‐glycan structure, eight
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Ectopic enhancer–enhancer interactions as causal forces driving RNA-directed DNA methylation in gene regulatory regions Plant Biotech. J. (IF 10.1) Pub Date : 2024-07-17 Yazhou Yang, Jia Liu, Stacy D. Singer, Guohua Yan, Dennis R. Bennet, Yue Liu, Jean-Michel Hily, Weirong Xu, Yingzhen Yang, Xiping Wang, Gan-Yuan Zhong, Zhongchi Liu, Yong-Chiang An, Huawei Liu, Zongrang Liu
Cis-regulatory elements (CREs) are integral to the spatiotemporal and quantitative expression dynamics of target genes, thus directly influencing phenotypic variation and evolution. However, many of these CREs become highly susceptible to transcriptional silencing when in a transgenic state, particularly when organised as tandem repeats. We investigated the mechanism of this phenomenon and found that
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Knockout of ZmNST2 promotes bioethanol production from corn stover Plant Biotech. J. (IF 10.1) Pub Date : 2024-07-15 Ying Wang, Ye Xing, Xinyu Yang, Yanwen Yu, Jiankun Li, Chenyang Zhao, Mengyu Yuan, Weili Huang, Yue Yin, Guohui Liu, Yuqing Sun, Haochuan Li, Jihua Tang, Qin Zhang, Mingyue Gou
The crude oil crisis causes an increasing demand of renewable energy, among which, bioethanol is considered the cleanest and renewable liquid fuel alternative to fossil fuel (An Tran et al., 2019). Bioethanol was mostly produced from sugarcane and corn, which violates vigorously against the world's food security. Alternatively, efforts have been made to produce bioethanol from non-food lignocellulose
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Development of a multi‐resistance and high‐yield rice variety using multigene transformation and gene editing Plant Biotech. J. (IF 10.1) Pub Date : 2024-07-14 Changyan Li, Zaihui Zhou, Xinzhu Xiong, Chuanxu Li, Chuanhong Li, Enlong Shen, Jianyu Wang, Wenjun Zha, Bian Wu, Hao Chen, Lei Zhou, Yongjun Lin, Aiqing You
Approximately one-third of the total annual food production in the world is lost owing to pests, diseases and weeds. Therefore, the challenges posed by crop losses and population growth have emphasized the need for better breeding techniques (FAO et al., 2023). Practical experience has demonstrated that the utilization of existing resistance genes to breed and cultivate herbicide- and pest-resistant
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Precision editing of a susceptibility gene promoter to alter its methylation modification for engineering rice resilience to biotic and abiotic stresses Plant Biotech. J. (IF 10.1) Pub Date : 2024-07-14 Jingjing Tian, Hang Zhang, Shuxin Li, Yongjun Lin, Lizhong Xiong, Meng Yuan
Rice is a primary food crop, and its yield is threatened by biotic and abiotic stresses. Xanthomonas oryzae pv. oryzae (Xoo) causes bacterial blight, a chief bacterial disease of rice. Xoo infects rice depending on its transcriptional activation-like effectors (TALEs), which specifically target effector binding elements (EBEs) in the promoter of host susceptibility (S) genes and regulate S genes' expression
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Cotton BOP1 mediates SUMOylation of GhBES1 to regulate fibre development and plant architecture Plant Biotech. J. (IF 10.1) Pub Date : 2024-07-14 Bingting Wang, Zhian Wang, Ye Tang, Naiqin Zhong, Jiahe Wu
SummaryThe Arabidopsis BLADE‐ON‐PETIOLE (BOP) genes are primarily known for their roles in regulating leaf and floral patterning. However, the broader functions of BOPs in regulating plant traits remain largely unexplored. In this study, we investigated the role of the Gossypium hirsutum BOP1 gene in the regulation of fibre length and plant height through the brassinosteroid (BR) signalling pathway
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CRISPR/Cas9-mediated editing of Bs5 and Bs5L in tomato leads to resistance against Xanthomonas Plant Biotech. J. (IF 10.1) Pub Date : 2024-07-12 Arturo Ortega, Kyungyong Seong, Alex Schultink, Daniela Paula de Toledo Thomazella, Eunyoung Seo, Elaine Zhang, Julie Pham, Myeong-Je Cho, Douglas Dahlbeck, Jacqueline Warren, Gerald V. Minsavage, Jeffrey B. Jones, Edgar Sierra-Orozco, Samuel F. Hutton, Brian Staskawicz
Bacterial spot, caused by Xanthomonas species, is a devastating disease of tomato (Solanum lycopersicum) and pepper (Capsicum annuum) (Schwartz et al., 2015). The recessively inherited resistance, bacterial spot 5 (bs5), in pepper (hereafter referred to as Cabs5) can confer resistance against different Xanthomonas strains (Jones et al., 2002). The Cabs5 resistance is characterized by the absence of
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Just FIND‐IT: Harnessing the true power of induced mutagenesis Plant Biotech. J. (IF 10.1) Pub Date : 2024-07-09 Christoph Dockter, Søren Knudsen, Magnus Wohlfahrt Rasmussen, Birgitte Skadhauge, Birger Lindberg Møller
In nature, genetic variation occurs in every population and results in the evolution of a diversity of new properties, some of which promote the survival of the species. To accelerate nature's evolution based on genetic diversity, plant breeders may induce additional mutations to raise the number of genetic variations increasing the chances to obtain varieties with new desired traits like improved
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Bt corn and cotton planting may benefit peanut growers by reducing aflatoxin risk Plant Biotech. J. (IF 10.1) Pub Date : 2024-07-08 Jina Yu, David A. Hennessy, Felicia Wu
SummaryDecades of studies have shown that Bt corn, by reducing insect damage, has lower levels of mycotoxins (fungal toxins), such as aflatoxin and fumonisin, than conventional corn. We used crop insurance data to infer that this benefit from Bt crops extends to reducing aflatoxin risk in peanuts: a non‐Bt crop. In consequence, we suggest that any benefit–cost assessment of how transgenic Bt crops
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Fine‐tuning the N‐glycosylation of recombinant human erythropoietin using Chlamydomonas reinhardtii mutants Plant Biotech. J. (IF 10.1) Pub Date : 2024-07-05 S. Leprovost, C. Plasson, J. Balieu, M‐L. Walet‐Balieu, P. Lerouge, M. Bardor, E. Mathieu‐Rivet
SummaryMicroalgae are considered as attractive expression systems for the production of biologics. As photosynthetic unicellular organisms, they do not require costly and complex media for growing and are able to secrete proteins and perform protein glycosylation. Some biologics have been successfully produced in the green microalgae Chlamydomonas reinhardtii. However, post‐translational modifications
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Mutation of the Vinv 5′ UTR regulatory region reduces acrylamide levels in processed potato to reach EU food-safety standards Plant Biotech. J. (IF 10.1) Pub Date : 2024-07-01 Leonard Shumbe, Emanoella Soares, Yordan Muhovski, Inga Smit, Hervé Vanderschuren
The recent prohibition of Chlorpropham (CIPC) in the EU (Commission Implementing Regulation(EU) 2019/989) is prompting the potato processing industry to search for alternative and safer anti-sprouting approaches. Storage at cold temperature (i.e. 4 °C) has emerged as a valuable option for long term storage of potato without the use of CIPC. However, most commercial potato varieties accumulate high
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Enhancing peanut nutritional quality by editing AhKCS genes lacking natural variation Plant Biotech. J. (IF 10.1) Pub Date : 2024-07-01 Dongxin Huai, Xiaomeng Xue, Jie Wu, Manish K. Pandey, Nian Liu, Li Huang, Liying Yan, Yuning Chen, Xin Wang, Qianqian Wang, Yanping Kang, Zhihui Wang, Huifang Jiang, Rajeev K. Varshney, Boshou Liao, Yong Lei
Peanut (Arachis hypogaea L.) is a globally staple oilseed crop, extensively cultivated in tropical and subtropical regions. Due to its substantial oil (approximately 46%–58%) and protein (around 22%–32%) content, the peanut plays a pivotal role in addressing malnutrition and ensuring food security in many regions. The fatty acid profiles of vegetable oil and foods have recently garnered increased attention
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Natural variation of WBR7 confers rice high yield and quality by modulating sucrose supply in sink organs Plant Biotech. J. (IF 10.1) Pub Date : 2024-06-29 Huan Shi, Peng Yun, Yun Zhu, Lu Wang, Yipei Wang, Pingbo Li, Hao Zhou, Shiyuan Cheng, Rongjia Liu, Guanjun Gao, Qinglu Zhang, Jinghua Xiao, Yibo Li, Lizhong Xiong, Aiqing You, Yuqing He
SummaryGrain chalkiness is an undesirable trait that negatively regulates grain yield and quality in rice. However, the regulatory mechanism underlying chalkiness is complex and remains unclear. We identified a positive regulator of white‐belly rate (WBR). The WBR7 gene encodes sucrose synthase 3 (SUS3). A weak functional allele of WBR7 is beneficial in increasing grain yield and quality. During the
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A miniature alternative to Cas9 and Cas12: Transposon-associated TnpB mediates targeted genome editing in plants Plant Biotech. J. (IF 10.1) Pub Date : 2024-06-28 Subhasis Karmakar, Debasmita Panda, Sonali Panda, Manaswini Dash, Romio Saha, Priya Das, S.P. Avinash, Justin Shih, Yinong Yang, A. K. Nayak, Mirza J. Baig, Kutubuddin A. Molla
The two popular genome editor nucleases, Cas9 and Cas12a, hypothetically evolved from IscB and TnpB, respectively (Altae-Tran et al., 2021). Recent reports showed that TnpBs also function as RNA-guided nucleases in human cells (Karvelis et al., 2021). TnpB proteins are much smaller (~400 aa) than Cas9 (~1000–1400 aa) and Cas12a (~1300 aa). The large cargo size of Cas9 and Cas12a hinders their delivery
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The fnr‐like mutants confer isoxaben tolerance by initiating mitochondrial retrograde signalling Plant Biotech. J. (IF 10.1) Pub Date : 2024-06-27 Ronan C. Broad, Michael Ogden, Arka Dutta, Peter M. Dracatos, James Whelan, Staffan Persson, Ghazanfar Abbas Khan
SummaryIsoxaben is a pre‐emergent herbicide used to control broadleaf weeds. While the phytotoxic mechanism is not completely understood, isoxaben interferes with cellulose synthesis. Certain mutations in cellulose synthase complex proteins can confer isoxaben tolerance; however, these mutations can cause compromised cellulose synthesis and perturbed plant growth, rendering them unsuitable as herbicide
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Delineation of loci governing an extra-earliness trait in lentil (Lens culinaris Medik.) using the QTL-Seq approach Plant Biotech. J. (IF 10.1) Pub Date : 2024-06-25 Kumbarahally Murthigowda Shivaprasad, Harsh K. Dikshit, Gyan Prakash Mishra, Subodh Kumar Sinha, Muraleedhar Aski, Manju Kohli, Dwijesh C. Mishra, Amit Kumar Singh, Soma Gupta, Akanksha Singh, Kuldeep Tripathi, Ranjeet Ranjan Kumar, Atul Kumar, Girish Kumar Jha, Shiv Kumar, Rajeev K. Varshney
Developing early maturing lentil has the potential to minimize yield losses, mainly during terminal drought. Whole-genome resequencing (WGRS) based QTL-seq identified the loci governing earliness in lentil. The genetic analysis for maturity duration provided a good fit to 3:1 segregation (F2), indicating earliness as a recessive trait. WGRS of Globe Mutant (late parent), late-flowering, and early-flowering
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A CRISPR/Cas9‐mediated mutant library of seed‐preferred genes in rice Plant Biotech. J. (IF 10.1) Pub Date : 2024-06-25 Dongsheng Zhao, Siyu Chen, Yangshuo Han, Guanqing Liu, Jinyu Liu, Qingqing Yang, Tao Zhang, Jilei Shen, Xiaolei Fan, Changquan Zhang, Tao Zhang, Qianfeng Li, Chen Chen, Qiaoquan Liu
Rice seeds are an important energy source for humans. Seed traits are difficult to observe and controlled by complex networks. Therefore, mutant libraries enriched in seed traits are vital for interpreting gene functions during seed development as well as grain yield and quality formation. Using the simple and efficient genomic editing tool, several CRISPR/Cas9-based mutant libraries have been generated
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The complex hexaploid oil-Camellia genome traces back its phylogenomic history and multi-omics analysis of Camellia oil biosynthesis Plant Biotech. J. (IF 10.1) Pub Date : 2024-06-24 Huaguo Zhu, Fuqiu Wang, Zhongping Xu, Guanying Wang, Lisong Hu, Junyong Cheng, Xianhong Ge, Jinxuan Liu, Wei Chen, Qiang Li, Fei Xue, Feng Liu, Wenying Li, Lan Wu, Xinqi Cheng, Xinxin Tang, Chaochen Yang, Keith Lindsey, Xianlong Zhang, Fang Ding, Haiyan Hu, Xiaoming Hu, Shuangxia Jin
Oil-Camellia (Camellia oleifera), belonging to the Theaceae family Camellia, is an important woody edible oil tree species. The Camellia oil in its mature seed kernels, mainly consists of more than 90% unsaturated fatty acids, tea polyphenols, flavonoids, squalene and other active substances, which is one of the best quality edible vegetable oils in the world. However, genetic research and molecular