The use of natural polymers and electrospinning as a new method of wound dressing production is one of the things that can revolutionize the medical world. Due to the importance of wound healing and characteristics such as anti-inflammatory and antimicrobial properties, it is possible to use natural compounds such as fungi and metabolites derived from them to produce wound dressing. In this study, schizophyllan (SPG) as an extracellular polysaccharide was extracted from Iranian Schizophyllum commune (NCBI MG761830) and then the silver nanoparticles (AgNPs) were produced by the in-situ method in 1.5 % SPG solution. Afterward, they were combined with polyvinyl alcohol 10 % (PVA) polymer to strengthen the fiber structure. We investigated the properties of nanofibers containing PVA/SPG-AgNPs and PVA/SPG20 %. The physicochemical properties of two fibers were investigated by SEM, TEM, FTIR, contact angle, water uptake, nanoparticle release, and biological test (antibacterial, and MTT). The diameter of the nanofiber-containing the AgNPs was about 169 nm and the other nanofiber was about 212 nm. The highest inhibition of the growth of the bacterium by PVA/SPG-AgNPs against E. coli and S. aureus was about 88.34 % and 64.7 %, respectively. The silver ion release from PVA/SPG-AgNPs nanofibers was 21 μg/ml after fifth day. Both nanofibers had no toxic effect on L929 fibroblast cells.

中文翻译：

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PVA / Schizophyllan-AgNPs纳米纤维的原位制备作为伤口愈合的潜力：表征和细胞毒性。

天然聚合物和静电纺丝作为伤口敷料生产的新方法的使用是可以彻底改变医学界的事物之一。由于伤口愈合的重要性和诸如抗炎和抗微生物特性的特性，可以使用天然化合物（例如真菌和衍生自它们的代谢产物）来生产伤口敷料。在这项研究中，裂殖体（SPG）是一种细胞外多糖，是从伊朗裂殖体公社中提取的。（NCBI MG761830），然后通过原位方法在1.5％SPG溶液中制备银纳米颗粒（AgNPs）。之后，将它们与10％聚乙烯醇（PVA）聚合物结合以增强纤维结构。我们研究了含有PVA / SPG-AgNPs和PVA / SPG20％的纳米纤维的性能。通过SEM，TEM，FTIR，接触角，吸水率，纳米颗粒释放和生物学测试（抗菌和MTT）研究了两种纤维的理化性质。包含纳米纤维的AgNP的直径为约169nm，另一根纳米纤维为约212nm。PVA / SPG-AgNPs对大肠杆菌和金黄色葡萄球菌对细菌生长的抑制作用最高分别约为88.34％和64.7％。第五天后，从PVA / SPG-AgNPs纳米纤维释放的银离子为21μg/ ml。两种纳米纤维均对L929成纤维细胞没有毒性作用。