An easily prepared biosensor based on reduced graphene oxide (rGO) and glucose oxidase (GOx) enzyme was developed to monitor the enzymatic hydrolysis process of the second-generation (2G) ethanol process from green coconut biomass. The rGO-GOx biocomposite that modified a glassy carbon (GC) electrode was characterized by morphological, electrochemical and spectrophotometric techniques showing that the GOx enzyme was immobilized on the rGO. The parameters for glucose determination were optimized by square wave voltammetry (SWV). The developed biosensor was applied for the determination of glucose during the enzymatic hydrolysis step, showing that the process can be stopped with 12 h of reaction. Thus, an important achievement of this analysis is the reduced time to get a valuable result for the test, saving time and reducing the cost of the 2G ethanol process.

开发了一种易于制备的基于还原性氧化石墨烯（rGO）和葡萄糖氧化酶（GOx）酶的生物传感器，以监测绿色椰子生物质中第二代（2G）乙醇工艺的酶水解过程。修饰玻碳电极的rGO-GOx生物复合材料的形态，电化学和分光光度技术表明，GOx酶固定在rGO上。用于葡萄糖测定的参数通过方波伏安法（SWV）进行了优化。研发的生物传感器用于酶水解步骤中葡萄糖的测定，表明该过程可以在12小时的反应后停止。因此，此分析的一项重要成就是减少了获得有价值的测试结果的时间，