Ultrasound-assisted synthesis of chiral cysteine-capped CdSe quantum dots for fluorometric differentiation and quantitation of tryptophan enantiomers,Microchimica Acta

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Ultrasound-assisted synthesis of chiral cysteine-capped CdSe quantum dots for fluorometric differentiation and quantitation of tryptophan enantiomers
Microchimica Acta ( IF 5.3 ) Pub Date : 2019-12-19 , DOI: 10.1007/s00604-019-4046-9
Saber Zare ₁ , Javad Tashkhourian ₁

Affiliation

A room temperature ultrasound-assisted method was applied to synthesize L- and D-cysteine-capped CdSe quantum dots (QDs). The QDs were characterized by XRD, FT-IR, and TEM. They have diameters of 5–7 nm and are shown to be viable probes for highly selective chiral recognition of tryptophan (Trp) enantiomers by fluorometry. The green fluorescence of the capped QDs (with excitation/emission maxima at 380/527 nm and 380/520 nm for L-Cys and D-Cys QDs, respectively) is differently quenched by D- and L-Trp in a high selective manner, with negligible interference by other species. The calibration plots and corresponding Stern-Volmer plots for both Trp enantiomers were investigated by two different approaches: In the first, each individual enantiomer was tested. In the second, each enantiomer was tested in the presence of a 100-folds excess of the other enantiomer. The detection limits for the recognition of L- and D-Trp are 4.2 and 4.7 nM, respectively, for the first approach. In the presence of the other enantiomer, the LODs are 4.4 and 4.8 nM. The linear range extends from 0.1 to 15 μM for both enantiomers. Graphical abstract Schematic representation of tryptophan (Trp) chiral recognition process. The fluorescence (green, ON) of D- and L-Cys (cysteine)-capped CdSe QDs is quenched (black, OFF) through a preferential and selective interaction with L- and D-Trp, respectively. Schematic representation of tryptophan (Trp) chiral recognition process. The fluorescence (green, ON) of D- and L-Cys (cysteine)-capped CdSe QDs is quenched (black, OFF) through a preferential and selective interaction with L- and D-Trp, respectively.

中文翻译：

超声辅助合成手性半胱氨酸封端的 CdSe 量子点，用于色氨酸对映体的荧光区分和定量

应用室温超声辅助方法合成 L-和 D-半胱氨酸封端的 CdSe 量子点 (QD)。通过 XRD、FT-IR 和 TEM 对 QD 进行表征。它们的直径为 5-7 nm，并且被证明是通过荧光测定法对色氨酸 (Trp) 对映体进行高度选择性手性识别的可行探针。加帽 QD 的绿色荧光（L-Cys 和 D-Cys QD 的激发/发射最大值分别为 380/527 nm 和 380/520 nm）被 D-和 L-Trp 以高选择性方式猝灭，其他物种的干扰可以忽略不计。通过两种不同的方法研究了两种 Trp 对映体的校准图和相应的 Stern-Volmer 图：首先，测试每个单独的对映体。在第二，在另一种对映体过量 100 倍的情况下测试每种对映体。对于第一种方法，L-和 D-Trp 识别的检测限分别为 4.2 和 4.7 nM。在存在其他对映异构体的情况下，LOD 为 4.4 和 4.8 nM。两种对映异构体的线性范围从 0.1 到 15 μM。色氨酸 (Trp) 手性识别过程的图形摘要示意图。D- 和 L-Cys（半胱氨酸）封端的 CdSe QD 的荧光（绿色，ON）分别通过与 L- 和 D-Trp 的优先和选择性相互作用被淬灭（黑色，OFF）。色氨酸 (Trp) 手性识别过程的示意图。D-和 L-Cys（半胱氨酸）封端的 CdSe QD 的荧光（绿色，ON）通过与 L-和 D-Trp 的优先和选择性相互作用被淬灭（黑色，OFF），

更新日期：2019-12-19

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