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Quantitative proteomic analysis reveals that the Rap1/MAPK/ERK pathway is inhibited through selenomethionine strengthening antioxidant activity.
Biometals ( IF 4.1 ) Pub Date : 2019-12-13 , DOI: 10.1007/s10534-019-00229-w
Zhe Liu 1 , Feng Zhang 2 , Lina Cui 3 , Jihong Wang 1 , Ping Lu 4 , Rui Zhao 1 , Hua Zhang 1 , Jianfa Wang 5 , Chunqiu Li 1 , Rui Wu 5
Affiliation  

To investigate the influence on the proteome of chicken skeletal muscles of Selenomethionine (SeMet) use, 36 chicks were fed with SeMet feeding for 35 days. A total of 72 1-day old broiler chicks were randomly allocated into two groups (n = 36/group): the control group (C group), the SeMet supplemented group (SeMet group). The Selenium (Se) concentrations of skeletal muscles from the chicks with basal diet (negative control group) and SeMet feeding were found to be 0.01 mg/kg and 0.40 mg/kg, respectively. The skeletal muscles from the two groups were investigated using isobaric Tags for Relative and Absolute Quantitation (iTRAQ), coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. This proteomic analysis identified proteins that were differentially expressed between the two groups. A total of 3564 proteins from the SeMet and the control (C) groups at 35 days were analyzed. 86 proteins were found by iTRAQ to be differentially expressed in the SeMet group, including 38 up-regulated proteins and 48 down-regulated proteins. These differential proteins were later identified as being mainly involved in antioxidant and enzyme-regulating activities. Fluorescent quantitative PCR(qPCR) and Western blot analyse proved to be consistent with the results of iTRAQ identification. The differentially expressed proteins (DEPs) identified in our work could be specific biomarkers related to SeMet intake in chicks. SeMet intake may strengthen antioxidant activity through Rap1/mitogen-activated protein kinases (MAPK)/extracellular signal-regulated kinase (ERK) signal pathways.

中文翻译:

定量蛋白质组学分析表明,Rap1 / MAPK / ERK途径通过硒代蛋氨酸增强抗氧化活性而受到抑制。

为了研究硒代蛋氨酸(SeMet)的使用对鸡骨骼肌蛋白质组的影响,我们对36只小鸡进行了SeMet喂养,喂养了35天。将总共​​72只1日龄的肉仔鸡随机分为两组(n = 36 /组):对照组(C组),SeMet补充组(SeMet组)。基础饮食(阴性对照组)和SeMet喂养的雏鸡骨骼肌的硒(Se)浓度分别为0.01 mg / kg和0.40 mg / kg。使用相对定量和绝对定量(iTRAQ)的等压标记,以及液相色谱-串联质谱(LC-MS / MS)分析,对两组的骨骼肌进行了研究。蛋白质组学分析鉴定了两组之间差异表达的蛋白质。在35天时,对来自SeMet和对照组(C)的总共3564种蛋白质进行了分析。通过iTRAQ发现SeMet组中有86种蛋白质差异表达,其中包括38种上调蛋白和48种下调蛋白。这些差异蛋白后来被鉴定为主要参与抗氧化剂和酶的调节活性。荧光定量PCR(qPCR)和蛋白质印迹分析证明与iTRAQ鉴定结果一致。在我们的工作中发现的差异表达蛋白(DEP)可能是与小鸡SeMet摄入量相关的特定生物标志物。SeMet摄入可通过Rap1 /丝裂原激活的蛋白激酶(MAPK)/细胞外信号调节激酶(ERK)信号通路增强抗氧化活性。通过iTRAQ发现SeMet组中有86种蛋白质差异表达,其中包括38种上调蛋白和48种下调蛋白。这些差异蛋白后来被鉴定为主要参与抗氧化剂和酶的调节活性。荧光定量PCR(qPCR)和蛋白质印迹分析证明与iTRAQ鉴定结果一致。在我们的工作中发现的差异表达蛋白(DEP)可能是与小鸡SeMet摄入量相关的特定生物标志物。SeMet摄入可通过Rap1 /丝裂原激活的蛋白激酶(MAPK)/细胞外信号调节激酶(ERK)信号通路增强抗氧化活性。通过iTRAQ发现SeMet组中有86种蛋白质差异表达,其中包括38种上调蛋白和48种下调蛋白。这些差异蛋白后来被鉴定为主要参与抗氧化剂和酶的调节活性。荧光定量PCR(qPCR)和蛋白质印迹分析证明与iTRAQ鉴定结果一致。在我们的工作中发现的差异表达蛋白(DEP)可能是与小鸡SeMet摄入量相关的特定生物标志物。SeMet摄入可通过Rap1 /丝裂原激活的蛋白激酶(MAPK)/细胞外信号调节激酶(ERK)信号通路增强抗氧化活性。这些差异蛋白后来被鉴定为主要参与抗氧化剂和酶的调节活性。荧光定量PCR(qPCR)和蛋白质印迹分析证明与iTRAQ鉴定结果一致。在我们的工作中发现的差异表达蛋白(DEP)可能是与小鸡SeMet摄入量相关的特定生物标记。SeMet摄入可通过Rap1 /丝裂原激活的蛋白激酶(MAPK)/细胞外信号调节激酶(ERK)信号通路增强抗氧化活性。这些差异蛋白后来被鉴定为主要参与抗氧化剂和酶的调节活性。荧光定量PCR(qPCR)和蛋白质印迹分析证明与iTRAQ鉴定结果一致。在我们的工作中发现的差异表达蛋白(DEP)可能是与小鸡SeMet摄入量相关的特定生物标志物。SeMet摄入可通过Rap1 /丝裂原激活的蛋白激酶(MAPK)/细胞外信号调节激酶(ERK)信号通路增强抗氧化活性。在我们的工作中发现的差异表达蛋白(DEP)可能是与小鸡SeMet摄入量相关的特定生物标志物。SeMet摄入可通过Rap1 /丝裂原激活的蛋白激酶(MAPK)/细胞外信号调节激酶(ERK)信号通路增强抗氧化活性。在我们的工作中发现的差异表达蛋白(DEP)可能是与小鸡SeMet摄入量相关的特定生物标记。SeMet摄入可通过Rap1 /丝裂原激活的蛋白激酶(MAPK)/细胞外信号调节激酶(ERK)信号通路增强抗氧化活性。
更新日期:2020-04-20
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