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Quantitative proteomic analysis reveals that the Rap1/MAPK/ERK pathway is inhibited through selenomethionine strengthening antioxidant activity

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Abstract

To investigate the influence on the proteome of chicken skeletal muscles of Selenomethionine (SeMet) use, 36 chicks were fed with SeMet feeding for 35 days. A total of 72 1-day old broiler chicks were randomly allocated into two groups (n = 36/group): the control group (C group), the SeMet supplemented group (SeMet group). The Selenium (Se) concentrations of skeletal muscles from the chicks with basal diet (negative control group) and SeMet feeding were found to be 0.01 mg/kg and 0.40 mg/kg, respectively. The skeletal muscles from the two groups were investigated using isobaric Tags for Relative and Absolute Quantitation (iTRAQ), coupled with liquid chromatography-tandem mass spectrometry (LC–MS/MS) analysis. This proteomic analysis identified proteins that were differentially expressed between the two groups. A total of 3564 proteins from the SeMet and the control (C) groups at 35 days were analyzed. 86 proteins were found by iTRAQ to be differentially expressed in the SeMet group, including 38 up-regulated proteins and 48 down-regulated proteins. These differential proteins were later identified as being mainly involved in antioxidant and enzyme-regulating activities. Fluorescent quantitative PCR(qPCR) and Western blot analyse proved to be consistent with the results of iTRAQ identification. The differentially expressed proteins (DEPs) identified in our work could be specific biomarkers related to SeMet intake in chicks. SeMet intake may strengthen antioxidant activity through Rap1/mitogen-activated protein kinases (MAPK)/extracellular signal-regulated kinase (ERK) signal pathways.

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Acknowledgements

This work was supported by grants from the National key research and development program of the 13th 5-year plan (2016YFD0501203), Foundation of National Natural Science Project, P.R. China (31472249;31772789), Foundation Project of Heilongjiang province natural (No. H2017038), and the Startup Foundation for the Doctors in Heilongjiang Bayi Agricultural University (Grant No. XDB-2017-18). Heilongjiang Bayi Agricultural University Team Support Program for San Heng San Zong (TDJH201905) and Heilongjiang Bayi Agricultural University Support Program for San Heng San Zong (ZRCPY201909). The authors wish to thank the study participants and Jilin University for their contributions to this study. All of the authors have read the manuscript and have agreed to submit it, in its current form, for consideration for publication.

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Author notes

  1. The contribution of Zhe Liu, Feng Zhang and Lina Cui co-together is equally important in this article.

Authors and Affiliations

  1. College of Life Sciences and Biotechnology, Heilongjiang Bayi Agricultural University, 2 Xinyang Road, Daqing, 163319, Heilongjiang, People’s Republic of China

    Zhe Liu, Jihong Wang, Rui Zhao, Hua Zhang & Chunqiu Li

  2. Department of Osteology, The Daqing Oil Field General Hospital, Daqing, 163319, Heilongjiang, People’s Republic of China

    Feng Zhang

  3. Yanbian University Hospital, Jilin, China

    Lina Cui

  4. China Animal Health and Epidemiology Center, Qingdao, 266000, People’s Republic of China

    Ping Lu

  5. College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing High-Tech Industrial Development Zone, Daqing, 163319, Heilongjiang, People’s Republic of China

    Jianfa Wang & Rui Wu

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  1. Zhe Liu
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Liu, Z., Zhang, F., Cui, L. et al. Quantitative proteomic analysis reveals that the Rap1/MAPK/ERK pathway is inhibited through selenomethionine strengthening antioxidant activity. Biometals 33, 45–64 (2020). https://doi.org/10.1007/s10534-019-00229-w

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