Regenerative medicine proposes to regenerate or even replace human damaged tissues to return to normal functions. Hence, biomaterials have been used to provide appropriate environment for cell development. Among the groups of biodegradable biomaterials, hydrogels, which are characterized by three-dimensional and cross-linked networks of water-soluble polymers, have been highlighted as suitable matrices for such applications. An injectable hydrogel based on oxidized galactomannan (OxGM) from Delonix regia and N-succinyl chitosan (NSC) was developed and characterized according to its physicochemical and biocompatible properties. The hydrogel was formed by Schiff base (-CH = N-) cross-linking between aldehyde groups from OxGM and NH2 groups from NSC, in few minutes (9.7 min) without any external stimulus. A hydrogel with macroporous structure, interconnected pores, and porosity of 69% was obtained. The biomaterial exhibited excellent injectability. No change in volume or integrity was observed in the hydrogel after its swelling in phosphate buffered saline (PBS) medium. This is an important property because when the hydrogel is injected into the site of interest and it fills the environment, it will not have additional space to occupy. Biocompatibility studies were conducted in vitro, which revealed the non-cytotoxic nature of the material and demonstrated the potential of the hydrogel based on dialdehyde galactomannan and N-succinyl chitosan for cell culture and soft tissue engineering.

中文翻译：

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基于二醛半乳甘露聚糖和N-琥珀酰壳聚糖的可注射水凝胶：细胞培养的合适平台。

再生医学建议再生甚至替换人类受损的组织以恢复正常功能。因此，生物材料已被用来为细胞发育提供适当的环境。在可生物降解的生物材料组中，以水溶性聚合物的三维和交联网络为特征的水凝胶，已被强调为适用于此类应用的基质。开发了一种基于Delonix regia氧化半乳甘露聚糖（OxGM）和N-琥珀酰壳聚糖（NSC）的可注射水凝胶，并根据其理化性质和生物相容性对其进行了表征。水凝胶是在几分钟（9.7分钟）内，无任何外部刺激的情况下，通过Oxiff的醛基和NSC的NH2基团之间的席夫碱（-CH = N-）交联形成的。具有大孔结构的水凝胶，连通的孔，孔隙率达到69％。该生物材料显示出优异的可注射性。水凝胶在磷酸盐缓冲盐水（PBS）介质中溶胀后，未观察到体积或完整性的变化。这是重要的性质，因为当将水凝胶注入目标部位并充满环境时，它将没有额外的空间可容纳。在体外进行了生物相容性研究，该研究揭示了该材料的非细胞毒性性质，并证明了基于二醛半乳甘露聚糖和N-琥珀酰壳聚糖的水凝胶在细胞培养和软组织工程中的潜力。水凝胶在磷酸盐缓冲盐水（PBS）介质中溶胀后，未观察到体积或完整性的变化。这是重要的性质，因为当将水凝胶注入目标部位并充满环境时，它将没有额外的空间可容纳。在体外进行了生物相容性研究，该研究揭示了该材料的非细胞毒性性质，并证明了基于二醛半乳甘露聚糖和N-琥珀酰壳聚糖的水凝胶在细胞培养和软组织工程中的潜力。水凝胶在磷酸盐缓冲盐水（PBS）介质中溶胀后，未观察到体积或完整性的变化。这是重要的性质，因为当将水凝胶注入目标部位并充满环境时，它将没有额外的空间可容纳。在体外进行了生物相容性研究，该研究揭示了该材料的非细胞毒性性质，并证明了基于二醛半乳甘露聚糖和N-琥珀酰壳聚糖的水凝胶在细胞培养和软组织工程中的潜力。