In the present study, a chitosan-based, multifunctional and double-faced barrier membrane was developed for the periodontitis therapy. The porous surface of the membrane was coated with bone-like hydroxyapatite (HA) produced by microwave-assisted biomimetic method and enriched with bone morphogenetic factor 6 (BMP-6) to enhance the bioactivity of chitosan. This surface of the membrane was designed to be in contact with the hard tissue that was damaged due to periodontitis. Otherwise the nonporous surface of membrane, which is in contact with the inflammatory soft tissue, was coated with electrospun polycaprolactone (PCL) fibers to prevent the migration of epithelial cells to the defect area. PrestoBlue, Scanning Electron Microscope (SEM) and real-time PCR results demonstrated that while porous surface of the membrane was enhancing the proliferation and differentiation of MC3T3-E1 preosteoblasts, nonporous surface of membrane did not allow migration of epithelial Madine Darby Bovine Kidney (MDBK) cells. The barrier membrane developed here is biodegradable and can be easily manipulated, has osteogenic activity and inactivity for epithelial cells. Thus, by implanting this membrane to the damaged periodontal tissue, bone regeneration will take place and integrity of periodontal tissues will be preserved.

中文翻译：

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基于壳聚糖的双面阻隔膜，涂有功能纳米结构并装有BMP-6。

在本研究中，开发了一种基于壳聚糖的多功能双面屏障膜，用于牙周炎治疗。膜的多孔表面涂有通过微波仿生法生产的骨状羟基磷灰石（HA），并富含骨形态发生因子6（BMP-6），以增强壳聚糖的生物活性。膜的该表面设计成与由于牙周炎而受损的硬组织接触。否则，将与炎症性软组织接触的膜的无孔表面涂上电纺聚己内酯（PCL）纤维，以防止上皮细胞迁移到缺损区域。PrestoBlue，扫描电子显微镜（SEM）和实时PCR结果表明，尽管膜的多孔表面增强了MC3T3-E1前成骨细胞的增殖和分化，但膜的无孔表面却不允许上皮Madine Darby牛肾（MDBK）细胞迁移。此处形成的屏障膜是可生物降解的，并且易于操作，对上皮细胞具有成骨活性和无活性。因此，通过将该膜植入受损的牙周组织，将发生骨再生并且将保持牙周组织的完整性。具有上皮细胞的成骨活性和无活性。因此，通过将该膜植入受损的牙周组织，将发生骨再生并且将保持牙周组织的完整性。具有上皮细胞的成骨活性和无活性。因此，通过将该膜植入受损的牙周组织，将发生骨再生并且将保持牙周组织的完整性。